MD13G1113000.v1.1

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001080
Physical Location & Seq
Forward (+)
8211021 .. 8212952
1932 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1113000.v1.1.491

Sequence Viewer

Length: 1509 bp
ATGGATTTCTTGAGTTATTGCTTGATAATCCCATGTCTTCTCTCTCTCTTGTATTTCTCAGTTCAAGCTTTCAGATCCGATCCCAAAAGGCTTCTGCTTCCACCTGGACCAAAGCCATTTCCCATCATTGGCAATCTCTTGGAGCTCGGCAACAAACCCCATCTCTCTCTCACTAACCTTTCACAACGATACGGCCCCATTATTTCATTGCAACTTGGACAAGTAACCACGGTCGTAGTTTCTTCATCAACCGTGGCAAAGGAGGTCCTCCGAACCCACGACCAGTTCTTGTGCAACCGAACCGTCCCAGACGCAATCCAAGCTTGCGATCTCCGCCAGGACAGCTTGCCCTGGATACCGGTGTCAGAGAAATGGAGAAACCTCCGTAAAATATGCAACAACCAATTGTTTGCAACAAAAGTCCTAGACGCCAACCAAGCCAACCGTCACCTGAAAGTGCAGGAGCTCATCGCCGATGTCAATGAAAGCGTTGTGAAAGGTAAGGCGGTAGAAATTGGGAGGGCTGCTTTTAAGACAACGCTCAATCTGCTGTCGCGTACTGTTTTCTCGGTCGATTTAGCTGACCCGAGCAGTGAGATGGGCAGAGAGTTCAAGGAGATTGTGTGGGGTTTGATGGAAGAGGCAGGGAAGCCAAACTTGGGGGACTATTTTCCTCTGCTTAGGAAACTTGACCCCCAAGGCATTCGACGGCGCATGATCAAACATTTCGTTAAGATTGATTCGCTTTTTGATCGAATGATTACGCAAAGGTTAGAATTGAGAAAATCGAACTATTATGTCACAAGTAATGATATGTTGGATTCTCTCTTGGACATCAGTGAAGTGAGCAATGAGGATGTGGACAAGCTCTTGATTGAACATCTGCTTTTGGCTCTATTTGTTGCGGGCACGGATACAACTTCAGCCACATTGGAATGGGCAATGGCTGAACTACTACGCAACCCGGAAAAACTGTTCAAAGCGCAAGAGGAACTAGAGCAAGTCATTGGCAAAGGAAAACCAGTTGAGGAATCGGACATTGCTCGACTCCCTTACTTACAAGCAATAATCAAAGAGACCTTCCGGTTGCACCCGGCTGTTCCATTACTACTCCCTCGAAAAGCCAAACAAGATGTGGAAATTTGCGGGTACATTGTCCCAAAGGGTGCACAAGTGCTGGTCAATGCATGGGCTATAGGCAGAGACCCCTGCATTTGGGACAACCCAACCTCATTTGTACCGGAGAGGTTTTTCGGATTAGATGATCAAATCGATGTTTTAGGCAAGAATTTTGAGCTTATTCCGTTTGGTGGAGGGAGGAGAATATGTCCAGGCCTGCCATTGGCGATGCGGATGCTACACTTGATGTTGGGTTCACTTATTAACTCATTTGATTGGAAGCTTGAAGATGGAGTTATACCAGAGAACATGAACATGGAGGAGAAGTTTGGCCTCACCTTACAGATGGCTCATCCTCTAAGAGCTGTGCCGAAAAAAGTCCTAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0008150 GO:0008152 GO:0009058 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0044550 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

503

Amino Acids

56.7

Weight (kDa)

7.08

Isoelectric Point (pI)

33.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 34 - 484 3.3e-103 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 556
AciI CCGC 5 cut(s) 334, 506, 905, 1146, 1351
AclWI GGATC 2 cut(s) 69, 74
AcsI RAATTY 2 cut(s) 1140, 1288
AcuI CTGAAG 1 cut(s) 906
AcyI GRCGYC 1 cut(s) 429
AfaI GTAC 3 cut(s) 559, 1151, 1239
AfiI CCNNNNNNNGG 5 cut(s) 128, 659, 1215, 1310, 1342
AgeI ACCGGT 1 cut(s) 358
AgsI TTSAA 5 cut(s) 65, 613, 878, 979, 1406
AjnI CCWGG 4 cut(s) 103, 336, 350, 1330
AjuI GAANNNNNNNTTGG 2 cut(s) 641, 673
AloI GAACNNNNNNTCC 2 cut(s) 959, 991
Alw21I GWGCWC 3 cut(s) 147, 468, 1171
Alw26I GTCTC 2 cut(s) 1070, 1197
Alw44I GTGCAC 1 cut(s) 1167
AlwI GGATC 2 cut(s) 69, 74
Ama87I CYCGRG 1 cut(s) 586
AoxI GGCC 3 cut(s) 193, 1333, 1450
ApaLI GTGCAC 1 cut(s) 1167
ApeKI GCWGC 1 cut(s) 524
ApoI RAATTY 2 cut(s) 1140, 1288
AsiGI ACCGGT 1 cut(s) 358
AspLEI GCGC 2 cut(s) 714, 985
AspS9I GGNCC 3 cut(s) 107, 194, 265
AsuC2I CCSGG 2 cut(s) 965, 1094
AsuHPI GGTGA 2 cut(s) 440, 1447
AvaI CYCGRG 1 cut(s) 586
AvaII GGWCC 2 cut(s) 107, 265
BaeGI GKGCMC 2 cut(s) 911, 1171
BaeI ACNNNNGTAYC 2 cut(s) 347, 380
BanII GRGCYC 2 cut(s) 147, 468
BbsI GAAGAC 1 cut(s) 29
Bbv12I GWGCWC 3 cut(s) 147, 468, 1171
BbvI GCAGC 1 cut(s) 511
BccI CCATC 6 cut(s) 131, 168, 592, 628, 1403, 1459
BceAI ACGGC 2 cut(s) 208, 725
BcgI CGANNNNNNTGC 2 cut(s) 1336, 1370
BciT130I CCWGG 4 cut(s) 105, 338, 352, 1332
BciVI GTATCC 2 cut(s) 348, 907
BclI TGATCA 2 cut(s) 717, 1264
BcnI CCSGG 2 cut(s) 965, 1094
BcoDI GTCTC 2 cut(s) 1070, 1197
BfaI CTAG 2 cut(s) 425, 995
BfmI CTRYAG 1 cut(s) 1194
BfuI GTATCC 2 cut(s) 348, 907
BisI GCNGC 1 cut(s) 525
BlsI GCNGC 1 cut(s) 526
Bme1390I CCNGG 6 cut(s) 105, 338, 352, 965, 1094, 1332
Bme18I GGWCC 2 cut(s) 107, 265
BmeT110I CYCGRG 1 cut(s) 586
BmgT120I GGNCC 3 cut(s) 107, 194, 265
BmiI GGNNCC 1 cut(s) 196
BmrFI CCNGG 6 cut(s) 105, 338, 352, 965, 1094, 1332
BmsI GCATC 2 cut(s) 1338, 1344
BpiI GAAGAC 1 cut(s) 29
Bpu10I CCTNAGC 1 cut(s) 680
BpuEI CTTGAG 1 cut(s) 31
BpuMI CCSGG 2 cut(s) 965, 1094
Bsa29I ATCGAT 1 cut(s) 1272
BsaHI GRCGYC 1 cut(s) 429
BsaI GGTCTC 2 cut(s) 1070, 1197
BsaJI CCNNGG 4 cut(s) 228, 252, 350, 697
BsaWI WCCGGW 3 cut(s) 358, 1083, 1240
BsaXI ACNNNNNCTCC 6 cut(s) 608, 638, 1312, 1342, 1430, 1460
Bsc4I CCNNNNNNNGG 5 cut(s) 128, 659, 1215, 1310, 1342
Bse118I RCCGGY 1 cut(s) 358
Bse1I ACTGG 2 cut(s) 283, 1022
Bse3DI GCAATG 4 cut(s) 206, 856, 948, 1038
BseBI CCWGG 4 cut(s) 105, 338, 352, 1332
BseCI ATCGAT 1 cut(s) 1272
BseDI CCNNGG 4 cut(s) 228, 252, 350, 697
BseGI GGATG 3 cut(s) 862, 1359, 1471
BseLI CCNNNNNNNGG 5 cut(s) 128, 659, 1215, 1310, 1342
BseMI GCAATG 4 cut(s) 206, 856, 948, 1038
BseMII CTCAG 1 cut(s) 72
BseNI ACTGG 2 cut(s) 283, 1022
BseRI GAGGAG 2 cut(s) 1333, 1454
BseSI GKGCMC 2 cut(s) 911, 1171
BseXI GCAGC 1 cut(s) 511
BsgI GTGCAG 1 cut(s) 479
Bsh1236I CGCG 1 cut(s) 556
Bsh1285I CGRYCG 2 cut(s) 234, 573
BshFI GGCC 3 cut(s) 195, 1335, 1452
BshTI ACCGGT 1 cut(s) 358
BshVI ATCGAT 1 cut(s) 1272
BsiEI CGRYCG 2 cut(s) 234, 573
BsiHKAI GWGCWC 3 cut(s) 147, 468, 1171
BsiHKCI CYCGRG 1 cut(s) 586
BsiSI CCGG 5 cut(s) 359, 965, 1084, 1094, 1241
BslFI GGGAC 4 cut(s) 290, 677, 1142, 1232
BslI CCNNNNNNNGG 5 cut(s) 128, 659, 1215, 1310, 1342
BsmAI GTCTC 2 cut(s) 1070, 1197
BsmFI GGGAC 4 cut(s) 290, 677, 1142, 1232
BsmI GAATGC 1 cut(s) 702
BsnI GGCC 3 cut(s) 195, 1335, 1452
Bso31I GGTCTC 2 cut(s) 1070, 1197
BsoBI CYCGRG 1 cut(s) 586
Bsp1286I GDGCHC 4 cut(s) 147, 468, 911, 1171
Bsp143I GATC 6 cut(s) 74, 79, 328, 717, 751, 1264
BspACI CCGC 5 cut(s) 334, 506, 905, 1146, 1351
BspANI GGCC 3 cut(s) 195, 1335, 1452
BspCNI CTCAG 1 cut(s) 71
BspDI ATCGAT 1 cut(s) 1272
BspFNI CGCG 1 cut(s) 556
BspLI GGNNCC 1 cut(s) 196
BspPI GGATC 2 cut(s) 69, 74
BspTNI GGTCTC 2 cut(s) 1070, 1197
BsrDI GCAATG 4 cut(s) 206, 856, 948, 1038
BsrFI RCCGGY 1 cut(s) 358
BsrI ACTGG 2 cut(s) 283, 1022
BssAI RCCGGY 1 cut(s) 358
BssECI CCNNGG 4 cut(s) 228, 252, 350, 697
BssMI GATC 6 cut(s) 74, 79, 328, 717, 751, 1264
BssNI GRCGYC 1 cut(s) 429
BssT1I CCWWGG 1 cut(s) 697
Bst2UI CCWGG 4 cut(s) 105, 338, 352, 1332
Bst4CI ACNGT 6 cut(s) 232, 253, 304, 446, 562, 975
Bst6I CTCTTC 1 cut(s) 633
BstACI GRCGYC 1 cut(s) 429
BstC8I GCNNGC 4 cut(s) 325, 347, 907, 1337
BstDEI CTNAG 3 cut(s) 58, 680, 1478
BstDSI CCRYGG 2 cut(s) 228, 252
BstF5I GGATG 3 cut(s) 862, 1359, 1471
BstFNI CGCG 1 cut(s) 556
BstHHI GCGC 2 cut(s) 714, 985
BstKTI GATC 6 cut(s) 77, 82, 331, 720, 754, 1267
BstMAI GTCTC 2 cut(s) 1070, 1197
BstMBI GATC 6 cut(s) 74, 79, 328, 717, 751, 1264
BstMCI CGRYCG 2 cut(s) 234, 573
BstMWI GCNNNNNNNGC 5 cut(s) 320, 333, 342, 437, 547
BstNI CCWGG 4 cut(s) 105, 338, 352, 1332
BstSCI CCNGG 6 cut(s) 103, 336, 350, 963, 1092, 1330
BstSFI CTRYAG 1 cut(s) 1194
BstSLI GKGCMC 2 cut(s) 911, 1171
BstUI CGCG 1 cut(s) 556
BstV1I GCAGC 1 cut(s) 511
BstV2I GAAGAC 1 cut(s) 29
BstX2I RGATCY 1 cut(s) 74
BstYI RGATCY 1 cut(s) 74
Bsu15I ATCGAT 1 cut(s) 1272
BsuI GTATCC 2 cut(s) 348, 907
BsuRI GGCC 3 cut(s) 195, 1335, 1452
BsuTUI ATCGAT 1 cut(s) 1272
BtgI CCRYGG 2 cut(s) 228, 252
BtgZI GCGATG 2 cut(s) 454, 1361
BtsCI GGATG 3 cut(s) 862, 1359, 1471
BtsI GCAGTG 1 cut(s) 598
BtsIMutI CAGTG 2 cut(s) 598, 844
Cac8I GCNNGC 4 cut(s) 325, 347, 907, 1337
CfoI GCGC 2 cut(s) 714, 985
Cfr10I RCCGGY 1 cut(s) 358
Cfr13I GGNCC 3 cut(s) 107, 194, 265
ClaI ATCGAT 1 cut(s) 1272
CseI GACGC 2 cut(s) 320, 437
Csp6I GTAC 3 cut(s) 558, 1150, 1238
CspAI ACCGGT 1 cut(s) 358
CviAII CATG 5 cut(s) 33, 715, 1188, 1429, 1435
CviQI GTAC 3 cut(s) 558, 1150, 1238
DdeI CTNAG 3 cut(s) 58, 680, 1478
DpnI GATC 6 cut(s) 76, 81, 330, 719, 753, 1266
DpnII GATC 6 cut(s) 74, 79, 328, 717, 751, 1264
Eam1104I CTCTTC 1 cut(s) 633
EarI CTCTTC 1 cut(s) 633
EciI GGCGGA 1 cut(s) 323
Ecl136II GAGCTC 2 cut(s) 145, 466
Eco130I CCWWGG 1 cut(s) 697
Eco147I AGGCCT 1 cut(s) 1335
Eco24I GRGCYC 2 cut(s) 147, 468
Eco31I GGTCTC 2 cut(s) 1070, 1197
Eco47I GGWCC 2 cut(s) 107, 265
Eco53kI GAGCTC 2 cut(s) 145, 466
Eco57I CTGAAG 1 cut(s) 906
Eco88I CYCGRG 1 cut(s) 586
EcoICRI GAGCTC 2 cut(s) 145, 466
EcoO109I RGGNCCY 1 cut(s) 265
EcoRII CCWGG 4 cut(s) 103, 336, 350, 1330
EcoT14I CCWWGG 1 cut(s) 697
EcoT22I ATGCAT 1 cut(s) 1189
EcoT38I GRGCYC 2 cut(s) 147, 468
ErhI CCWWGG 1 cut(s) 697
FaeI CATG 5 cut(s) 36, 718, 1191, 1432, 1438
FalI AAGNNNNNCTT 2 cut(s) 641, 673
FaqI GGGAC 4 cut(s) 290, 677, 1142, 1232
FatI CATG 5 cut(s) 32, 714, 1187, 1428, 1434
FauI CCCGC 2 cut(s) 898, 1139
FbaI TGATCA 2 cut(s) 717, 1264
Fnu4HI GCNGC 1 cut(s) 525
FokI GGATG 3 cut(s) 869, 1366, 1458
FriOI GRGCYC 2 cut(s) 147, 468
Fsp4HI GCNGC 1 cut(s) 525
FspBI CTAG 2 cut(s) 425, 995
GlaI GCGC 2 cut(s) 713, 984
GluI GCNGC 1 cut(s) 525
HaeIII GGCC 3 cut(s) 195, 1335, 1452
HapII CCGG 5 cut(s) 359, 965, 1084, 1094, 1241
HgaI GACGC 2 cut(s) 320, 437
HhaI GCGC 2 cut(s) 714, 985
Hin1I GRCGYC 1 cut(s) 429
Hin1II CATG 5 cut(s) 36, 718, 1191, 1432, 1438
Hin6I GCGC 2 cut(s) 712, 983
HinP1I GCGC 2 cut(s) 712, 983
HindIII AAGCTT 3 cut(s) 66, 321, 1400
HinfI GANTC 4 cut(s) 740, 821, 1031, 1047
HpaII CCGG 5 cut(s) 359, 965, 1084, 1094, 1241
HphI GGTGA 2 cut(s) 440, 1447
Hpy166II GTNNAC 3 cut(s) 862, 1169, 1376
Hpy188I TCNGA 6 cut(s) 74, 79, 272, 367, 1036, 1256
Hpy188III TCNNGA 2 cut(s) 10, 871
Hpy8I GTNNAC 3 cut(s) 862, 1169, 1376
Hpy99I CGWCG 1 cut(s) 711
HpyAV CCTTC 1 cut(s) 1090
HpyCH4III ACNGT 6 cut(s) 232, 253, 304, 446, 562, 975
HpyCH4V TGCA 9 cut(s) 211, 294, 396, 413, 460, 1090, 1169, 1187, 1212
HpyF10VI GCNNNNNNNGC 5 cut(s) 320, 333, 342, 437, 547
HpyF3I CTNAG 3 cut(s) 58, 680, 1478
Hsp92I GRCGYC 1 cut(s) 429
Hsp92II CATG 5 cut(s) 36, 718, 1191, 1432, 1438
HspAI GCGC 2 cut(s) 712, 983
Ksp22I TGATCA 2 cut(s) 717, 1264
Kzo9I GATC 6 cut(s) 74, 79, 328, 717, 751, 1264
LmnI GCTCC 2 cut(s) 142, 463
Lsp1109I GCAGC 1 cut(s) 511
LweI GCATC 2 cut(s) 1338, 1344
MaeI CTAG 2 cut(s) 425, 995
MaeIII GTNAC 3 cut(s) 223, 446, 799
MalI GATC 6 cut(s) 76, 81, 330, 719, 753, 1266
MboI GATC 6 cut(s) 74, 79, 328, 717, 751, 1264
MboII GAAGA 4 cut(s) 29, 234, 650, 1418
MfeI CAATTG 1 cut(s) 404
MflI RGATCY 1 cut(s) 74
MhlI GDGCHC 4 cut(s) 147, 468, 911, 1171
MluCI AATT 6 cut(s) 404, 513, 776, 1140, 1288, 1503
MlyI GAGTC 1 cut(s) 1041
MmeI TCCRAC 1 cut(s) 798
Mph1103I ATGCAT 1 cut(s) 1189
MseI TTAA 3 cut(s) 531, 732, 1383
MslI CAYNNNNRTG 2 cut(s) 934, 1433
MspI CCGG 5 cut(s) 359, 965, 1084, 1094, 1241
MspR9I CCNGG 6 cut(s) 105, 338, 352, 965, 1094, 1332
MunI CAATTG 1 cut(s) 404
Mva1269I GAATGC 1 cut(s) 702
MvaI CCWGG 4 cut(s) 105, 338, 352, 1332
MvnI CGCG 1 cut(s) 556
MwoI GCNNNNNNNGC 5 cut(s) 320, 333, 342, 437, 547
NciI CCSGG 2 cut(s) 965, 1094
NdeII GATC 6 cut(s) 74, 79, 328, 717, 751, 1264
NlaIII CATG 5 cut(s) 36, 718, 1191, 1432, 1438
NlaIV GGNNCC 1 cut(s) 196
NmeAIII GCCGAG 1 cut(s) 126
NmuCI GTSAC 2 cut(s) 446, 799
NsiI ATGCAT 1 cut(s) 1189
PceI AGGCCT 1 cut(s) 1335
PctI GAATGC 1 cut(s) 702
PfeI GAWTC 3 cut(s) 740, 821, 1031
PinAI ACCGGT 1 cut(s) 358
PkrI GCNGC 1 cut(s) 526
PleI GAGTC 1 cut(s) 1041
PpsI GAGTC 1 cut(s) 1041
PpuMI RGGWCCY 1 cut(s) 265
Psp124BI GAGCTC 2 cut(s) 147, 468
Psp5II RGGWCCY 1 cut(s) 265
Psp6I CCWGG 4 cut(s) 103, 336, 350, 1330
PspGI CCWGG 4 cut(s) 103, 336, 350, 1330
PspN4I GGNNCC 1 cut(s) 196
PspPI GGNCC 3 cut(s) 107, 194, 265
PspPPI RGGWCCY 1 cut(s) 265
PsuI RGATCY 1 cut(s) 74
RsaI GTAC 3 cut(s) 559, 1151, 1239
RsaNI GTAC 3 cut(s) 558, 1150, 1238
RseI CAYNNNNRTG 2 cut(s) 934, 1433
SacI GAGCTC 2 cut(s) 147, 468
SaqAI TTAA 3 cut(s) 531, 732, 1383
SatI GCNGC 1 cut(s) 525
Sau3AI GATC 6 cut(s) 74, 79, 328, 717, 751, 1264
Sau96I GGNCC 3 cut(s) 107, 194, 265
SchI GAGTC 1 cut(s) 1041
ScrFI CCNGG 6 cut(s) 105, 338, 352, 965, 1094, 1332
SduI GDGCHC 4 cut(s) 147, 468, 911, 1171
SfaNI GCATC 2 cut(s) 1338, 1344
SfcI CTRYAG 1 cut(s) 1194
SinI GGWCC 2 cut(s) 107, 265
SmiMI CAYNNNNRTG 2 cut(s) 934, 1433
SmlI CTYRAG 1 cut(s) 10
SmoI CTYRAG 1 cut(s) 10
Sse9I AATT 6 cut(s) 404, 513, 776, 1140, 1288, 1503
SseBI AGGCCT 1 cut(s) 1335
SsiI CCGC 5 cut(s) 334, 506, 905, 1146, 1351
SspMI CTAG 2 cut(s) 425, 995
SstI GAGCTC 2 cut(s) 147, 468
StuI AGGCCT 1 cut(s) 1335
StyD4I CCNGG 6 cut(s) 103, 336, 350, 963, 1092, 1330
StyI CCWWGG 1 cut(s) 697
TaaI ACNGT 6 cut(s) 232, 253, 304, 446, 562, 975
TaqI TCGA 7 cut(s) 573, 706, 754, 788, 1045, 1117, 1272
TaqII GACCGA 1 cut(s) 559
TasI AATT 6 cut(s) 404, 513, 776, 1140, 1288, 1503
TfiI GAWTC 3 cut(s) 740, 821, 1031
Tru1I TTAA 3 cut(s) 531, 732, 1383
Tru9I TTAA 3 cut(s) 531, 732, 1383
TscAI CASTG 2 cut(s) 598, 844
TseFI GTSAC 2 cut(s) 446, 799
TseI GCWGC 1 cut(s) 524
Tsp45I GTSAC 2 cut(s) 446, 799
TspDTI ATGAA 4 cut(s) 195, 234, 498, 1445
TspGWI ACGGA 3 cut(s) 374, 926, 1293
TspRI CASTG 2 cut(s) 598, 844
VneI GTGCAC 1 cut(s) 1167
VpaK11BI GGWCC 2 cut(s) 107, 265
XapI RAATTY 2 cut(s) 1140, 1288
XcmI CCANNNNNNNNNTGG 1 cut(s) 1132
XspI CTAG 2 cut(s) 425, 995
Zsp2I ATGCAT 1 cut(s) 1189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.