AT2G45550

cytochrome P450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001080
Physical Location & Seq
Reverse (-)
18773517 .. 18775797
2281 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G45550.1

Sequence Viewer

Length: 1536 bp
ATGGACATCATCTCAGGGCAAGCTCTGTTTCTCCTCTTTTGCTTTATCTCATCATGTTTCCTCATCTCGACAACCGCAAGATCCCGACGGAGTTCTGGCCGAGCCGCCACGCTGCCTCCTGGACCTCCACGATTACCGATTATCGGAAACATTCACCAAGTCGGTAAAAACCCGCACAGCTCATTCGCTGACCTCGCAAAAATTTATGGACCAATCATGAGTCTTAAGTTTGGATGTCTAAACTCAGTCGTCATAACTTCACCAGAAGCTGCAAGAGAAGTACTAAGAACACACGACCAAATCTTGTCTGGCCGTAAGTCCAATGACTCTATACGGTGCTTTGGTCACGAAGAAGTTTCCGTCATCTGGCTTCCTCCGTCGTCCGCTCGTTGGAGGATGTTGAGAAAACTGTCAGTGACTCTCATGTTCTCACCGCAGCGTACCGAAGCCACTAAAGCTCTGCGGATGAAGAAAGTGCAAGAGCTTGTAAGCTTCATGAATGAAAGCAGCGAGAGAAAAGAAGCAGTTGATATTTCTCGTGCATCCTACACCACAGTTCTTAATATCATATCCAACATTCTGTTTTCGGTTGATCTCGGTAGCTATGACTCGAAAAAATCCAACGAATTTCAGGACACGGTGATTGGTGCCATGGAAGCTGCTGGGAAACCAGACGCTGCTAACTACTTTCCATTTATGGGGTTTCTTGACCTACAAGGTAATAGGAAGGCAATGAGGGGTCTGACGGAGAGGTTGTTTAGGGTTTTCCGTGGGTTCATGGATGCTAAAATAGCAGAAAAATCATTAGGGAATTACTCTAAAGATGTTTCCAACAGAGATTTCTTGGATTCGCTTCTCATCCTCAACGAAGGAGATGAAGCAGAGCTCGACAATAACGATATTGAACACCTACTCTTGGATATGTTTACAGCTGGCACAGATACAAGCTCTAGTACTCTAGAGTGGGCAATGGCAGAGTTACTTAGAAACCCTAAAACGATGGTAAAAGCTCAGGCAGAGATGGATCGTGTGTTAGGTCAAAACAGTGTTGTGCAAGAGTCAGATATCTCAGGATTGCCATATTTACAAGCGGTTGTGAAGGAAACTTTTAGGTTACATCCGGCTGCTCCGCTACTAGTCCCACGAAAAGCGGAATCAGATGTGGAGGTTCTTGGGTTCATGGTGCCTAAAGATACTCAGGTTCTAGTGAATGTGTGGGCCATAGGACGAGACCCAAGCGTATGGGAGAATCCGAGCCAGTTCGAGCCAGAGAGGTTTATGGGGAAAGATATTGATGTGAAAGGTAGAGATTATGAGCTTACACCGTTTGGAGGAGGACGGAGAATATGTCCGGGATTGCCTTTGGCTGTGAAAACAGTGTCTCTTATGCTTGCTTCTCTTCTTTATTCCTTTGACTGGAAGCTTCCTAACGGTGTCGTTTCAGAGGACTTGGATATGGACGAGACCTTTGGTATCACATTACACAGGACCAACACGTTATATGCCATTCCCGTAAAGAAACAGACCATTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0008150 GO:0008152 GO:0009058 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0044550 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

511

Amino Acids

56.93

Weight (kDa)

6.44

Isoelectric Point (pI)

39.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 39 - 497 1.2e-108 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 647, 1183
AccBSI CCGCTC 1 cut(s) 386
AciI CCGC 9 cut(s) 75, 105, 173, 384, 434, 463, 1091, 1130, 1151
AclWI GGATC 2 cut(s) 75, 1032
AcoI YGGCCR 2 cut(s) 97, 310
AcsI RAATTY 2 cut(s) 201, 626
AfaI GTAC 3 cut(s) 282, 442, 955
AfiI CCNNNNNNNGG 7 cut(s) 143, 366, 390, 698, 916, 1331, 1416
AflII CTTAAG 1 cut(s) 224
AflIII ACRYGT 1 cut(s) 1494
AgsI TTSAA 1 cut(s) 905
AhlI ACTAGT 1 cut(s) 1135
AjnI CCWGG 1 cut(s) 118
AjuI GAANNNNNNNTTGG 2 cut(s) 824, 856
Alw21I GWGCWC 1 cut(s) 888
Alw26I GTCTC 3 cut(s) 1224, 1386, 1457
AlwI GGATC 2 cut(s) 75, 1032
AlwNI CAGNNNCTG 2 cut(s) 269, 677
AoxI GGCC 3 cut(s) 97, 310, 1218
ApeKI GCWGC 7 cut(s) 112, 269, 436, 507, 659, 677, 1124
ApoI RAATTY 2 cut(s) 201, 626
ArsI GACNNNNNNTTYG 2 cut(s) 1451, 1483
AseI ATTAAT 1 cut(s) 1530
AspS9I GGNCC 4 cut(s) 122, 209, 1218, 1488
AsuC2I CCSGG 1 cut(s) 1353
AsuHPI GGTGA 4 cut(s) 146, 252, 423, 652
AvaII GGWCC 3 cut(s) 122, 209, 1488
BaeI ACNNNNGTAYC 2 cut(s) 1185, 1218
BanI GGYRCC 2 cut(s) 647, 1183
BanII GRGCYC 1 cut(s) 888
BauI CACGAG 1 cut(s) 537
Bbv12I GWGCWC 1 cut(s) 888
BbvI GCAGC 7 cut(s) 99, 256, 448, 519, 646, 664, 1111
BccI CCATC 2 cut(s) 994, 1015
BceAI ACGGC 1 cut(s) 297
BciT130I CCWGG 1 cut(s) 120
BcnI CCSGG 1 cut(s) 1353
BcoDI GTCTC 3 cut(s) 1224, 1386, 1457
BcuI ACTAGT 1 cut(s) 1135
BfaI CTAG 4 cut(s) 951, 959, 1136, 1205
BfrI CTTAAG 1 cut(s) 224
BisI GCNGC 8 cut(s) 105, 113, 270, 437, 508, 660, 678, 1125
BlsI GCNGC 8 cut(s) 106, 114, 271, 438, 509, 661, 679, 1126
BmcAI AGTACT 2 cut(s) 282, 955
Bme1390I CCNGG 2 cut(s) 120, 1353
Bme18I GGWCC 3 cut(s) 122, 209, 1488
BmgT120I GGNCC 4 cut(s) 122, 209, 1218, 1488
BmiI GGNNCC 2 cut(s) 649, 1185
BmrFI CCNGG 2 cut(s) 120, 1353
BmsI GCATC 2 cut(s) 551, 772
Bpu10I CCTNAGC 1 cut(s) 1011
BpuMI CCSGG 1 cut(s) 1353
BsaI GGTCTC 2 cut(s) 1224, 1457
BsaJI CCNNGG 2 cut(s) 651, 769
BsaXI ACNNNNNCTCC 6 cut(s) 100, 130, 740, 770, 1333, 1363
Bsc4I CCNNNNNNNGG 7 cut(s) 143, 366, 390, 698, 916, 1331, 1416
Bse1I ACTGG 2 cut(s) 1258, 1421
Bse3DI GCAATG 2 cut(s) 738, 975
BseBI CCWGG 1 cut(s) 120
BseDI CCNNGG 2 cut(s) 651, 769
BseGI GGATG 7 cut(s) 239, 402, 471, 542, 787, 858, 1117
BseLI CCNNNNNNNGG 7 cut(s) 143, 366, 390, 698, 916, 1331, 1416
BseMI GCAATG 2 cut(s) 738, 975
BseMII CTCAG 5 cut(s) 27, 258, 1025, 1083, 1211
BseNI ACTGG 2 cut(s) 1258, 1421
BseRI GAGGAG 2 cut(s) 23, 1347
BseXI GCAGC 7 cut(s) 99, 256, 448, 519, 646, 664, 1111
BseYI CCCAGC 1 cut(s) 662
BshFI GGCC 3 cut(s) 99, 312, 1220
BshNI GGYRCC 2 cut(s) 647, 1183
BsiHKAI GWGCWC 1 cut(s) 888
BsiSI CCGG 2 cut(s) 1121, 1352
BslFI GGGAC 1 cut(s) 1124
BslI CCNNNNNNNGG 7 cut(s) 143, 366, 390, 698, 916, 1331, 1416
BsmAI GTCTC 3 cut(s) 1224, 1386, 1457
BsmFI GGGAC 1 cut(s) 1124
BsnI GGCC 3 cut(s) 99, 312, 1220
Bso31I GGTCTC 2 cut(s) 1224, 1457
Bsp1286I GDGCHC 1 cut(s) 888
Bsp143I GATC 3 cut(s) 80, 592, 1024
Bsp19I CCATGG 1 cut(s) 651
BspACI CCGC 9 cut(s) 75, 105, 173, 384, 434, 463, 1091, 1130, 1151
BspANI GGCC 3 cut(s) 99, 312, 1220
BspCNI CTCAG 5 cut(s) 26, 257, 1024, 1082, 1210
BspHI TCATGA 2 cut(s) 216, 495
BspLI GGNNCC 2 cut(s) 649, 1185
BspPI GGATC 2 cut(s) 75, 1032
BspT107I GGYRCC 2 cut(s) 647, 1183
BspTI CTTAAG 1 cut(s) 224
BspTNI GGTCTC 2 cut(s) 1224, 1457
BsrBI CCGCTC 1 cut(s) 386
BsrDI GCAATG 2 cut(s) 738, 975
BsrI ACTGG 2 cut(s) 1258, 1421
BssECI CCNNGG 2 cut(s) 651, 769
BssMI GATC 3 cut(s) 80, 592, 1024
BssSI CACGAG 1 cut(s) 537
BssT1I CCWWGG 1 cut(s) 651
Bst2BI CACGAG 1 cut(s) 537
Bst2UI CCWGG 1 cut(s) 120
Bst4CI ACNGT 8 cut(s) 336, 411, 556, 640, 1046, 1326, 1378, 1433
Bst6I CTCTTC 1 cut(s) 1404
BstAFI CTTAAG 1 cut(s) 224
BstC8I GCNNGC 3 cut(s) 21, 934, 1392
BstDEI CTNAG 7 cut(s) 13, 244, 284, 983, 1011, 1069, 1197
BstDSI CCRYGG 2 cut(s) 651, 769
BstF5I GGATG 7 cut(s) 239, 402, 471, 542, 787, 858, 1117
BstKTI GATC 3 cut(s) 83, 595, 1027
BstMAI GTCTC 3 cut(s) 1224, 1386, 1457
BstMBI GATC 3 cut(s) 80, 592, 1024
BstMWI GCNNNNNNNGC 4 cut(s) 194, 455, 656, 791
BstNI CCWGG 1 cut(s) 120
BstSCI CCNGG 2 cut(s) 118, 1351
BstV1I GCAGC 7 cut(s) 99, 256, 448, 519, 646, 664, 1111
BstX2I RGATCY 1 cut(s) 80
BstXI CCANNNNNNTGG 1 cut(s) 1242
BstYI RGATCY 1 cut(s) 80
BsuRI GGCC 3 cut(s) 99, 312, 1220
BtgI CCRYGG 2 cut(s) 651, 769
BtsCI GGATG 7 cut(s) 239, 402, 471, 542, 787, 858, 1117
BtsIMutI CAGTG 3 cut(s) 420, 1051, 1383
Cac8I GCNNGC 3 cut(s) 21, 934, 1392
CaiI CAGNNNCTG 2 cut(s) 269, 677
CciI TCATGA 2 cut(s) 216, 495
Cfr13I GGNCC 4 cut(s) 122, 209, 1218, 1488
CseI GACGC 1 cut(s) 683
Csp6I GTAC 3 cut(s) 281, 441, 954
CviAII CATG 7 cut(s) 54, 217, 424, 496, 652, 778, 1180
CviQI GTAC 3 cut(s) 281, 441, 954
DdeI CTNAG 7 cut(s) 13, 244, 284, 983, 1011, 1069, 1197
DpnI GATC 3 cut(s) 82, 594, 1026
DpnII GATC 3 cut(s) 80, 592, 1024
EaeI YGGCCR 2 cut(s) 97, 310
Eam1104I CTCTTC 1 cut(s) 1404
EarI CTCTTC 1 cut(s) 1404
Ecl136II GAGCTC 1 cut(s) 886
Eco130I CCWWGG 1 cut(s) 651
Eco24I GRGCYC 1 cut(s) 888
Eco31I GGTCTC 2 cut(s) 1224, 1457
Eco32I GATATC 1 cut(s) 1066
Eco47I GGWCC 3 cut(s) 122, 209, 1488
Eco53kI GAGCTC 1 cut(s) 886
EcoICRI GAGCTC 1 cut(s) 886
EcoRII CCWGG 1 cut(s) 118
EcoRV GATATC 1 cut(s) 1066
EcoT14I CCWWGG 1 cut(s) 651
EcoT38I GRGCYC 1 cut(s) 888
ErhI CCWWGG 1 cut(s) 651
FaeI CATG 7 cut(s) 57, 220, 427, 499, 655, 781, 1183
FaqI GGGAC 1 cut(s) 1124
FatI CATG 7 cut(s) 53, 216, 423, 495, 651, 777, 1179
FauI CCCGC 1 cut(s) 180
Fnu4HI GCNGC 8 cut(s) 105, 113, 270, 437, 508, 660, 678, 1125
FokI GGATG 7 cut(s) 246, 409, 478, 529, 794, 845, 1104
FriOI GRGCYC 1 cut(s) 888
Fsp4HI GCNGC 8 cut(s) 105, 113, 270, 437, 508, 660, 678, 1125
FspBI CTAG 4 cut(s) 951, 959, 1136, 1205
GluI GCNGC 8 cut(s) 105, 113, 270, 437, 508, 660, 678, 1125
GsaI CCCAGC 1 cut(s) 666
HaeIII GGCC 3 cut(s) 99, 312, 1220
HapII CCGG 2 cut(s) 1121, 1352
HgaI GACGC 1 cut(s) 683
Hin1II CATG 7 cut(s) 57, 220, 427, 499, 655, 781, 1183
HindIII AAGCTT 2 cut(s) 490, 1421
HinfI GANTC 8 cut(s) 220, 326, 418, 608, 848, 1058, 1154, 1249
HpaII CCGG 2 cut(s) 1121, 1352
HphI GGTGA 4 cut(s) 146, 252, 423, 652
Hpy166II GTNNAC 1 cut(s) 927
Hpy188I TCNGA 6 cut(s) 146, 744, 1063, 1159, 1254, 1444
Hpy188III TCNNGA 9 cut(s) 67, 84, 217, 347, 496, 632, 707, 959, 1071
Hpy8I GTNNAC 1 cut(s) 927
Hpy99I CGWCG 2 cut(s) 90, 382
HpyAV CCTTC 3 cut(s) 721, 863, 1093
HpyCH4III ACNGT 8 cut(s) 336, 411, 556, 640, 1046, 1326, 1378, 1433
HpyCH4IV ACGT 1 cut(s) 1496
HpyCH4V TGCA 4 cut(s) 272, 478, 542, 1054
HpyF10VI GCNNNNNNNGC 4 cut(s) 194, 455, 656, 791
HpyF3I CTNAG 7 cut(s) 13, 244, 284, 983, 1011, 1069, 1197
HpySE526I ACGT 1 cut(s) 1496
Hsp92II CATG 7 cut(s) 57, 220, 427, 499, 655, 781, 1183
Kzo9I GATC 3 cut(s) 80, 592, 1024
LmnI GCTCC 1 cut(s) 1132
Lsp1109I GCAGC 7 cut(s) 99, 256, 448, 519, 646, 664, 1111
LweI GCATC 2 cut(s) 551, 772
MaeI CTAG 4 cut(s) 951, 959, 1136, 1205
MaeII ACGT 1 cut(s) 1496
MaeIII GTNAC 4 cut(s) 344, 415, 978, 1113
MalI GATC 3 cut(s) 82, 594, 1026
MbiI CCGCTC 1 cut(s) 386
MboI GATC 3 cut(s) 80, 592, 1024
MboII GAAGA 3 cut(s) 362, 481, 1391
MflI RGATCY 1 cut(s) 80
MhlI GDGCHC 1 cut(s) 888
MluCI AATT 4 cut(s) 201, 626, 811, 1531
MlyI GAGTC 5 cut(s) 229, 320, 412, 602, 1067
MmeI TCCRAC 4 cut(s) 371, 597, 645, 855
MseI TTAA 3 cut(s) 225, 561, 1530
MspA1I CMGCKG 1 cut(s) 932
MspCI CTTAAG 1 cut(s) 224
MspI CCGG 2 cut(s) 1121, 1352
MspR9I CCNGG 2 cut(s) 120, 1353
MvaI CCWGG 1 cut(s) 120
MwoI GCNNNNNNNGC 4 cut(s) 194, 455, 656, 791
NciI CCSGG 1 cut(s) 1353
NcoI CCATGG 1 cut(s) 651
NdeII GATC 3 cut(s) 80, 592, 1024
NlaIII CATG 7 cut(s) 57, 220, 427, 499, 655, 781, 1183
NlaIV GGNNCC 2 cut(s) 649, 1185
NmeAIII GCCGAG 1 cut(s) 125
NmuCI GTSAC 2 cut(s) 344, 415
PagI TCATGA 2 cut(s) 216, 495
PcsI WCGNNNNNNNCGW 1 cut(s) 894
PfeI GAWTC 3 cut(s) 848, 1154, 1249
PfoI TCCNGGA 2 cut(s) 118, 1351
PkrI GCNGC 8 cut(s) 106, 114, 271, 438, 509, 661, 679, 1126
PleI GAGTC 5 cut(s) 228, 320, 412, 602, 1066
PpsI GAGTC 5 cut(s) 228, 320, 412, 602, 1066
PshBI ATTAAT 1 cut(s) 1530
Psp124BI GAGCTC 1 cut(s) 888
Psp6I CCWGG 1 cut(s) 118
PspFI CCCAGC 1 cut(s) 662
PspGI CCWGG 1 cut(s) 118
PspN4I GGNNCC 2 cut(s) 649, 1185
PspPI GGNCC 4 cut(s) 122, 209, 1218, 1488
PstNI CAGNNNCTG 2 cut(s) 269, 677
PsuI RGATCY 1 cut(s) 80
PvuII CAGCTG 1 cut(s) 932
RsaI GTAC 3 cut(s) 282, 442, 955
RsaNI GTAC 3 cut(s) 281, 441, 954
SacI GAGCTC 1 cut(s) 888
SaqAI TTAA 3 cut(s) 225, 561, 1530
SatI GCNGC 8 cut(s) 105, 113, 270, 437, 508, 660, 678, 1125
Sau3AI GATC 3 cut(s) 80, 592, 1024
Sau96I GGNCC 4 cut(s) 122, 209, 1218, 1488
ScaI AGTACT 2 cut(s) 282, 955
SchI GAGTC 5 cut(s) 229, 320, 412, 602, 1067
ScrFI CCNGG 2 cut(s) 120, 1353
SduI GDGCHC 1 cut(s) 888
SfaNI GCATC 2 cut(s) 551, 772
SinI GGWCC 3 cut(s) 122, 209, 1488
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
SpeI ACTAGT 1 cut(s) 1135
Sse9I AATT 4 cut(s) 201, 626, 811, 1531
SsiI CCGC 9 cut(s) 75, 105, 173, 384, 434, 463, 1091, 1130, 1151
SspMI CTAG 4 cut(s) 951, 959, 1136, 1205
SstI GAGCTC 1 cut(s) 888
StyD4I CCNGG 2 cut(s) 118, 1351
StyI CCWWGG 1 cut(s) 651
TaaI ACNGT 8 cut(s) 336, 411, 556, 640, 1046, 1326, 1378, 1433
TaiI ACGT 1 cut(s) 1499
TaqI TCGA 4 cut(s) 68, 611, 888, 1263
TasI AATT 4 cut(s) 201, 626, 811, 1531
TatI WGTACW 2 cut(s) 280, 953
TauI GCSGC 1 cut(s) 107
TfiI GAWTC 3 cut(s) 848, 1154, 1249
Tru1I TTAA 3 cut(s) 225, 561, 1530
Tru9I TTAA 3 cut(s) 225, 561, 1530
TscAI CASTG 3 cut(s) 420, 1051, 1383
TseFI GTSAC 2 cut(s) 344, 415
TseI GCWGC 7 cut(s) 112, 269, 436, 507, 659, 677, 1124
Tsp45I GTSAC 2 cut(s) 344, 415
TspDTI ATGAA 7 cut(s) 482, 484, 512, 516, 766, 891, 1168
TspGWI ACGGA 6 cut(s) 103, 349, 366, 758, 761, 1354
TspRI CASTG 3 cut(s) 420, 1051, 1383
Vha464I CTTAAG 1 cut(s) 224
VpaK11BI GGWCC 3 cut(s) 122, 209, 1488
VspI ATTAAT 1 cut(s) 1530
XapI RAATTY 2 cut(s) 201, 626
XbaI TCTAGA 1 cut(s) 958
XcmI CCANNNNNNNNNTGG 1 cut(s) 305
XspI CTAG 4 cut(s) 951, 959, 1136, 1205
ZrmI AGTACT 2 cut(s) 282, 955
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.