Rroxscaffold_3G00256190

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001080
Physical Location & Seq
Reverse (-)
50579341 .. 50581427
2087 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00256190.1

Sequence Viewer

Length: 1500 bp
ATGAACTTCTGGAGTGCTTTCGTTTACCTTATCCTCACCTGGACATTGATCCAAACCCTCCTCTACTCAATTTCAAGAGCCAAAAACACCTCCAAAAAGCTTCCACCAGGTCCAAAACCGCTTCCAATCCTCGGAAACCTTCTCCTACTTGGTGACAAACCTCACAAATCTCTTGCCAAACTTGCCCAAATTCATGGCCCTTTAATGTCCCTAAAACTTGGACAAGTAACCTCCATAGTTGTTTCTTCATCAGCCATGGCCAAACAAGTCATGCAAACCCATGATCAATTCTTCTCCAACCGAACAATCCCCGATTCCATTCGAGCCCTTGATCACCACACTGTTGGCTTGCCATGGATCCCTGTCTCACCCCTCTGGAGAAACCTTAGAAAAATATGCAACACTCAGCTATTTGCCAACAAGATGCTTGATGCTAACCAAAACCTGCGACGCAAAAAAGTGGAGCAACTCCTTGAATCAGTCCACAAAAGCAGCCTGAGTGGGGAAGCAGTTGACTTTGGTAGGGCAGCTTTCACCACAACACTCAATTTGCTCTCTAATACAACTTTTTCAATTGATTTAGCAGACCCGAGTTCCAAAATGGCCAGGGAGTTCAAGGAGATGGTGCATAATATTATGATCGAGGCTGGAAAACCAAACTTGGGGGACTATTTTCCTGTGCTTAGGAAGATTGATCCTAATGGCAGAAGAAGGAGAATGATAAAGTATTTTGGAAAGATAATAGATTTAGTTGAAACAATAGTTCAGCAGAGGTTGGAGCTGAGAAAATCGACTGAATCTGCAACAAAGAACGATTTGTTGGATACCCTTCTCACTATTACAGAAGAAAACAGTGAGGAGCTCAACAAAAATCAACTTTATCATTTCATACTGGTTCTTTTTGTTGCAGGGTCAGACACAACTTCATCCACATTCCAATGGGCAATGGCAGAGCTCTTGCACAACCCTGAAGTTCTCTCAAAAGCACAATTAGAGCTTAACGAAGTAATTGGCAAAGGCAATCCTGTTGAGGAATCAGACATTACTAGACTACCTTACTTGCAAGCCATTATTAAAGAAACATTTCGTATGCACCCTGCAGTTCCTTTGCTACTTCCCCGAAAAGCCGAATCAGATGTAGAAATTGAAGGCTCCACAGTCCCAAATGGTGCACAATTGCTTGTTAATGCATGGGCCATTGGCAGAGACCCAAGTATATGGGATGACCCGGATTCATTCAAGCCGGAGAGATTTTTGGGGTCTGAACTTGATGTTAGAGGCCGGAATTTTGAACTTATTCCATTTGGAGCCGGCAGGAGAATATGTCCGGGCTTACCATTGGCAATCAGAATGTTGCACTTAATGTTGGGTTCTCTTATCCATTCCTTTGTTTGGAAGCTTGAAGATGGAGTTACACCAGAGAACATGAACATGGATGATAAGTTTGGACTCACCCTGGAGATGGCTAAACCCTTGAGAGCTATCCCTATCCCTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0008150 GO:0008152 GO:0009058 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0044550 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

499

Amino Acids

56.16

Weight (kDa)

8.95

Isoelectric Point (pI)

37.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 35 - 484 1.4e-106 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 453
AciI CCGC 1 cut(s) 119
AclWI GGATC 4 cut(s) 43, 352, 365, 689
AcoI YGGCCR 2 cut(s) 258, 603
AcsI RAATTY 2 cut(s) 189, 1285
AcuI CTGAAG 1 cut(s) 990
AfiI CCNNNNNNNGG 5 cut(s) 131, 502, 662, 1392, 1462
AgsI TTSAA 9 cut(s) 75, 476, 573, 616, 755, 1148, 1240, 1292, 1403
AjnI CCWGG 4 cut(s) 38, 106, 605, 1455
AjuI GAANNNNNNNTTGG 2 cut(s) 803, 835
AluBI AGCT 9 cut(s) 100, 409, 530, 781, 862, 955, 997, 1399, 1481
AluI AGCT 9 cut(s) 100, 409, 530, 781, 862, 955, 997, 1399, 1481
Alw21I GWGCWC 3 cut(s) 864, 957, 1174
Alw26I GTCTC 2 cut(s) 370, 1200
Alw44I GTGCAC 1 cut(s) 1170
AlwI GGATC 4 cut(s) 43, 352, 365, 689
Ama87I CYCGRG 1 cut(s) 589
AoxI GGCC 5 cut(s) 196, 258, 603, 1194, 1279
ApaLI GTGCAC 1 cut(s) 1170
ApeKI GCWGC 2 cut(s) 492, 527
ApoI RAATTY 2 cut(s) 189, 1285
Asp700I GAANNNNTTC 2 cut(s) 1083, 1296
AspS9I GGNCC 3 cut(s) 110, 197, 1194
AsuC2I CCSGG 2 cut(s) 1229, 1329
AsuHPI GGTGA 6 cut(s) 28, 164, 326, 360, 526, 1444
AvaI CYCGRG 1 cut(s) 589
AvaII GGWCC 1 cut(s) 110
BaeGI GKGCMC 1 cut(s) 1174
BalI TGGCCA 2 cut(s) 260, 605
BamHI GGATCC 1 cut(s) 357
BanII GRGCYC 3 cut(s) 328, 864, 957
Bbv12I GWGCWC 3 cut(s) 864, 957, 1174
BbvI GCAGC 2 cut(s) 504, 539
BccI CCATC 3 cut(s) 616, 1400, 1456
BciT130I CCWGG 4 cut(s) 40, 108, 607, 1457
BciVI GTATCC 1 cut(s) 817
BclI TGATCA 2 cut(s) 283, 331
BcnI CCSGG 2 cut(s) 1229, 1329
BcoDI GTCTC 2 cut(s) 370, 1200
BfaI CTAG 1 cut(s) 1047
BfmI CTRYAG 1 cut(s) 1098
BfuAI ACCTGC 1 cut(s) 453
BfuI GTATCC 1 cut(s) 817
BisI GCNGC 2 cut(s) 493, 528
BlsI GCNGC 2 cut(s) 494, 529
Bme1390I CCNGG 6 cut(s) 40, 108, 607, 1229, 1329, 1457
Bme18I GGWCC 1 cut(s) 110
BmeT110I CYCGRG 1 cut(s) 589
BmgT120I GGNCC 3 cut(s) 110, 197, 1194
BmiI GGNNCC 3 cut(s) 359, 1153, 1309
BmrFI CCNGG 6 cut(s) 40, 108, 607, 1229, 1329, 1457
BmsI GCATC 2 cut(s) 414, 421
BpmI CTGGAG 3 cut(s) 31, 397, 1478
Bpu10I CCTNAGC 1 cut(s) 683
BpuEI CTTGAG 1 cut(s) 1495
BpuMI CCSGG 2 cut(s) 1229, 1329
BsaI GGTCTC 1 cut(s) 1200
BsaJI CCNNGG 5 cut(s) 130, 255, 353, 606, 1455
BsaXI ACNNNNNCTCC 2 cut(s) 1309, 1339
Bsc4I CCNNNNNNNGG 5 cut(s) 131, 502, 662, 1392, 1462
Bse118I RCCGGY 1 cut(s) 1310
Bse1I ACTGG 1 cut(s) 897
Bse3DI GCAATG 1 cut(s) 951
BseBI CCWGG 4 cut(s) 40, 108, 607, 1457
BseDI CCNNGG 5 cut(s) 130, 255, 353, 606, 1455
BseGI GGATG 3 cut(s) 926, 1228, 1441
BseLI CCNNNNNNNGG 5 cut(s) 131, 502, 662, 1392, 1462
BseMI GCAATG 1 cut(s) 951
BseMII CTCAG 3 cut(s) 419, 488, 773
BseNI ACTGG 1 cut(s) 897
BseRI GAGGAG 2 cut(s) 50, 872
BseSI GKGCMC 1 cut(s) 1174
BseXI GCAGC 2 cut(s) 504, 539
BshFI GGCC 5 cut(s) 198, 260, 605, 1196, 1281
BsiHKAI GWGCWC 3 cut(s) 864, 957, 1174
BsiHKCI CYCGRG 1 cut(s) 589
BsiSI CCGG 5 cut(s) 1229, 1244, 1282, 1311, 1328
BslFI GGGAC 3 cut(s) 193, 680, 1145
BslI CCNNNNNNNGG 5 cut(s) 131, 502, 662, 1392, 1462
BsmAI GTCTC 2 cut(s) 370, 1200
BsmFI GGGAC 3 cut(s) 193, 680, 1145
BsnI GGCC 5 cut(s) 198, 260, 605, 1196, 1281
Bso31I GGTCTC 1 cut(s) 1200
BsoBI CYCGRG 1 cut(s) 589
Bsp1286I GDGCHC 4 cut(s) 328, 864, 957, 1174
Bsp143I GATC 6 cut(s) 48, 283, 331, 357, 639, 694
Bsp19I CCATGG 2 cut(s) 255, 353
BspACI CCGC 1 cut(s) 119
BspANI GGCC 5 cut(s) 198, 260, 605, 1196, 1281
BspCNI CTCAG 3 cut(s) 418, 489, 774
BspLI GGNNCC 3 cut(s) 359, 1153, 1309
BspMAI CTGCAG 1 cut(s) 1102
BspMI ACCTGC 1 cut(s) 453
BspPI GGATC 4 cut(s) 43, 352, 365, 689
BspTNI GGTCTC 1 cut(s) 1200
BsrDI GCAATG 1 cut(s) 951
BsrFI RCCGGY 1 cut(s) 1310
BsrI ACTGG 1 cut(s) 897
BssAI RCCGGY 1 cut(s) 1310
BssECI CCNNGG 5 cut(s) 130, 255, 353, 606, 1455
BssMI GATC 6 cut(s) 48, 283, 331, 357, 639, 694
BssT1I CCWWGG 2 cut(s) 255, 353
Bst2UI CCWGG 4 cut(s) 40, 108, 607, 1457
Bst4CI ACNGT 3 cut(s) 343, 854, 1159
BstC8I GCNNGC 3 cut(s) 350, 1065, 1312
BstDEI CTNAG 5 cut(s) 386, 405, 497, 683, 782
BstDSI CCRYGG 2 cut(s) 255, 353
BstF5I GGATG 3 cut(s) 926, 1228, 1441
BstKTI GATC 6 cut(s) 51, 286, 334, 360, 642, 697
BstMAI GTCTC 2 cut(s) 370, 1200
BstMBI GATC 6 cut(s) 48, 283, 331, 357, 639, 694
BstMWI GCNNNNNNNGC 1 cut(s) 182
BstNI CCWGG 4 cut(s) 40, 108, 607, 1457
BstSCI CCNGG 6 cut(s) 38, 106, 605, 1227, 1327, 1455
BstSFI CTRYAG 1 cut(s) 1098
BstSLI GKGCMC 1 cut(s) 1174
BstV1I GCAGC 2 cut(s) 504, 539
BstX2I RGATCY 1 cut(s) 357
BstXI CCANNNNNNTGG 3 cut(s) 194, 344, 1218
BstYI RGATCY 1 cut(s) 357
BsuI GTATCC 1 cut(s) 817
BsuRI GGCC 5 cut(s) 198, 260, 605, 1196, 1281
BtgI CCRYGG 2 cut(s) 255, 353
BtsCI GGATG 3 cut(s) 926, 1228, 1441
BtsIMutI CAGTG 2 cut(s) 339, 859
BveI ACCTGC 1 cut(s) 453
Cac8I GCNNGC 3 cut(s) 350, 1065, 1312
Cfr10I RCCGGY 1 cut(s) 1310
Cfr13I GGNCC 3 cut(s) 110, 197, 1194
CseI GACGC 1 cut(s) 459
CsiI ACCWGGT 1 cut(s) 106
CviAII CATG 8 cut(s) 194, 256, 271, 281, 354, 1191, 1426, 1432
DdeI CTNAG 5 cut(s) 386, 405, 497, 683, 782
DpnI GATC 6 cut(s) 50, 285, 333, 359, 641, 696
DpnII GATC 6 cut(s) 48, 283, 331, 357, 639, 694
EaeI YGGCCR 2 cut(s) 258, 603
Ecl136II GAGCTC 2 cut(s) 862, 955
Eco130I CCWWGG 2 cut(s) 255, 353
Eco24I GRGCYC 3 cut(s) 328, 864, 957
Eco31I GGTCTC 1 cut(s) 1200
Eco47I GGWCC 1 cut(s) 110
Eco53kI GAGCTC 2 cut(s) 862, 955
Eco57I CTGAAG 1 cut(s) 990
Eco88I CYCGRG 1 cut(s) 589
EcoICRI GAGCTC 2 cut(s) 862, 955
EcoRII CCWGG 4 cut(s) 38, 106, 605, 1455
EcoT14I CCWWGG 2 cut(s) 255, 353
EcoT22I ATGCAT 1 cut(s) 1192
EcoT38I GRGCYC 3 cut(s) 328, 864, 957
ErhI CCWWGG 2 cut(s) 255, 353
FaeI CATG 8 cut(s) 197, 259, 274, 284, 357, 1194, 1429, 1435
FaqI GGGAC 3 cut(s) 193, 680, 1145
FatI CATG 8 cut(s) 193, 255, 270, 280, 353, 1190, 1425, 1431
FbaI TGATCA 2 cut(s) 283, 331
Fnu4HI GCNGC 2 cut(s) 493, 528
FokI GGATG 3 cut(s) 913, 1235, 1448
FriOI GRGCYC 3 cut(s) 328, 864, 957
Fsp4HI GCNGC 2 cut(s) 493, 528
FspBI CTAG 1 cut(s) 1047
GluI GCNGC 2 cut(s) 493, 528
GsuI CTGGAG 3 cut(s) 31, 397, 1478
HaeIII GGCC 5 cut(s) 198, 260, 605, 1196, 1281
HapII CCGG 5 cut(s) 1229, 1244, 1282, 1311, 1328
HgaI GACGC 1 cut(s) 459
Hin1II CATG 8 cut(s) 197, 259, 274, 284, 357, 1194, 1429, 1435
HincII GTYRAC 1 cut(s) 514
HindII GTYRAC 1 cut(s) 514
HindIII AAGCTT 2 cut(s) 98, 1397
HinfI GANTC 7 cut(s) 314, 476, 797, 1034, 1130, 1232, 1449
HpaII CCGG 5 cut(s) 1229, 1244, 1282, 1311, 1328
HphI GGTGA 6 cut(s) 28, 164, 326, 360, 526, 1444
Hpy166II GTNNAC 4 cut(s) 25, 484, 514, 1172
Hpy188I TCNGA 6 cut(s) 134, 916, 1039, 1135, 1264, 1349
Hpy188III TCNNGA 3 cut(s) 10, 75, 376
Hpy8I GTNNAC 4 cut(s) 25, 484, 514, 1172
Hpy99I CGWCG 1 cut(s) 453
HpyAV CCTTC 4 cut(s) 149, 705, 839, 1142
HpyCH4III ACNGT 3 cut(s) 343, 854, 1159
HpyF10VI GCNNNNNNNGC 1 cut(s) 182
HpyF3I CTNAG 5 cut(s) 386, 405, 497, 683, 782
Hsp92II CATG 8 cut(s) 197, 259, 274, 284, 357, 1194, 1429, 1435
KroI GCCGGC 1 cut(s) 1310
KroNI GCCGGC 1 cut(s) 1312
Ksp22I TGATCA 2 cut(s) 283, 331
Kzo9I GATC 6 cut(s) 48, 283, 331, 357, 639, 694
LmnI GCTCC 5 cut(s) 463, 778, 859, 1157, 1307
Lsp1109I GCAGC 2 cut(s) 504, 539
LweI GCATC 2 cut(s) 414, 421
MabI ACCWGGT 1 cut(s) 106
MaeI CTAG 1 cut(s) 1047
MaeIII GTNAC 3 cut(s) 152, 226, 1411
MalI GATC 6 cut(s) 50, 285, 333, 359, 641, 696
MboI GATC 6 cut(s) 48, 283, 331, 357, 639, 694
MboII GAAGA 6 cut(s) 237, 283, 700, 720, 857, 1415
MfeI CAATTG 2 cut(s) 573, 1175
MflI RGATCY 1 cut(s) 357
MhlI GDGCHC 4 cut(s) 328, 864, 957, 1174
MlsI TGGCCA 2 cut(s) 260, 605
MluNI TGGCCA 2 cut(s) 260, 605
MlyI GAGTC 1 cut(s) 1443
MmeI TCCRAC 3 cut(s) 321, 756, 801
Mox20I TGGCCA 2 cut(s) 260, 605
Mph1103I ATGCAT 1 cut(s) 1192
MroNI GCCGGC 1 cut(s) 1310
MroXI GAANNNNTTC 2 cut(s) 1083, 1296
MscI TGGCCA 2 cut(s) 260, 605
MseI TTAA 5 cut(s) 203, 999, 1074, 1185, 1361
MslI CAYNNNNRTG 2 cut(s) 937, 1430
Msp20I TGGCCA 2 cut(s) 260, 605
MspI CCGG 5 cut(s) 1229, 1244, 1282, 1311, 1328
MspR9I CCNGG 6 cut(s) 40, 108, 607, 1229, 1329, 1457
MunI CAATTG 2 cut(s) 573, 1175
MvaI CCWGG 4 cut(s) 40, 108, 607, 1457
MwoI GCNNNNNNNGC 1 cut(s) 182
NaeI GCCGGC 1 cut(s) 1312
NciI CCSGG 2 cut(s) 1229, 1329
NcoI CCATGG 2 cut(s) 255, 353
NdeII GATC 6 cut(s) 48, 283, 331, 357, 639, 694
NgoMIV GCCGGC 1 cut(s) 1310
NlaIII CATG 8 cut(s) 197, 259, 274, 284, 357, 1194, 1429, 1435
NlaIV GGNNCC 3 cut(s) 359, 1153, 1309
NmuCI GTSAC 1 cut(s) 152
NsiI ATGCAT 1 cut(s) 1192
PdiI GCCGGC 1 cut(s) 1312
PdmI GAANNNNTTC 2 cut(s) 1083, 1296
PfeI GAWTC 6 cut(s) 314, 476, 797, 1034, 1130, 1232
PkrI GCNGC 2 cut(s) 494, 529
PleI GAGTC 1 cut(s) 1443
PpsI GAGTC 1 cut(s) 1443
Psp124BI GAGCTC 2 cut(s) 864, 957
Psp6I CCWGG 4 cut(s) 38, 106, 605, 1455
PspGI CCWGG 4 cut(s) 38, 106, 605, 1455
PspN4I GGNNCC 3 cut(s) 359, 1153, 1309
PspPI GGNCC 3 cut(s) 110, 197, 1194
PstI CTGCAG 1 cut(s) 1102
PsuI RGATCY 1 cut(s) 357
RseI CAYNNNNRTG 2 cut(s) 937, 1430
SacI GAGCTC 2 cut(s) 864, 957
SaqAI TTAA 5 cut(s) 203, 999, 1074, 1185, 1361
SatI GCNGC 2 cut(s) 493, 528
Sau3AI GATC 6 cut(s) 48, 283, 331, 357, 639, 694
Sau96I GGNCC 3 cut(s) 110, 197, 1194
SchI GAGTC 1 cut(s) 1443
ScrFI CCNGG 6 cut(s) 40, 108, 607, 1229, 1329, 1457
SduI GDGCHC 4 cut(s) 328, 864, 957, 1174
SexAI ACCWGGT 1 cut(s) 106
SfaNI GCATC 2 cut(s) 414, 421
SfcI CTRYAG 1 cut(s) 1098
SinI GGWCC 1 cut(s) 110
SmiMI CAYNNNNRTG 2 cut(s) 937, 1430
SmlI CTYRAG 1 cut(s) 1474
SmoI CTYRAG 1 cut(s) 1474
SsiI CCGC 1 cut(s) 119
SspI AATATT 1 cut(s) 634
SspMI CTAG 1 cut(s) 1047
SstI GAGCTC 2 cut(s) 864, 957
StyD4I CCNGG 6 cut(s) 38, 106, 605, 1227, 1327, 1455
StyI CCWWGG 2 cut(s) 255, 353
TaaI ACNGT 3 cut(s) 343, 854, 1159
TaqI TCGA 3 cut(s) 322, 642, 791
TfiI GAWTC 6 cut(s) 314, 476, 797, 1034, 1130, 1232
Tru1I TTAA 5 cut(s) 203, 999, 1074, 1185, 1361
Tru9I TTAA 5 cut(s) 203, 999, 1074, 1185, 1361
TscAI CASTG 2 cut(s) 346, 859
TseFI GTSAC 1 cut(s) 152
TseI GCWGC 2 cut(s) 492, 527
Tsp45I GTSAC 1 cut(s) 152
TspDTI ATGAA 7 cut(s) 17, 182, 237, 877, 915, 1224, 1442
TspRI CASTG 2 cut(s) 346, 859
VneI GTGCAC 1 cut(s) 1170
VpaK11BI GGWCC 1 cut(s) 110
XapI RAATTY 2 cut(s) 189, 1285
XmnI GAANNNNTTC 2 cut(s) 1083, 1296
XspI CTAG 1 cut(s) 1047
Zsp2I ATGCAT 1 cut(s) 1192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.