AT3G61040

cytochrome P450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001080
Physical Location & Seq
Reverse (-)
22593973 .. 22596125
2153 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G61040.2

Sequence Viewer

Length: 1497 bp
ATGGATATTGTAGCAATAGTATTGTCTCTGCTCTTTATCTTCTTTCTCTTCTTCTTTTTCTACACCACCGGAAAATCATGTCCCGGTGGAGCCAAGAACCCACCGGGTCCTTCAAAGCTATCTCTGCTTCGTAACATCCTTCAAACTGTTGAAAAACCTCACCGTTCACTTGCCGATCTCTCAAGAATTTACGGAAGCGTTATGAGTTTTAAGCTTGGCTGTTTGACCACAGTGGTCATATCTTCACCGGAAACAGCAAAAGAAGTGCTGAAAACACATGATCATGTCTTGTCTTATCGAGTCTCCAGTGACCCGGTTCGAGCCGCTGGTCATCACGAGCTTTCCTTGCTCTGGATTCCTCCGTTGGCTCGTTGGAGGTTTCTACGAAAGATAACGAGGAACCAATTATTCTCAACGCAACGTCTAGAAGCAACCAGTGCTATAAGGACGAGGAAGGTGCAAGAACTCATGAACTTCGTTAACAAATGCTGCGAGAGACGAGAAGCTGTGAACATCTCTCGAGCATCATTCATCACATCACTCAATATAATCTCGAACGCTCTGTTTTCCACAAACCTCGCAAACTTTGATGACTCAAAGACATTTCACGATTTCCAAAACGTGGTGATTCGGATGATGGAGATCTCCGGAAAACCCAACCTCGCTGATTTCTTCCCTTTTCTTGGGTTTCTTGATCTGCAAGGAGCTAGAAAAGAAGCGAGGCTATTGATGCACAAACTGTTTAGGGTTTTTCAAGGATTCATCGATACAAAGAGATCATCAACTTCGAGAAATAACAACGATATGTTAGATTCTCTTTTGGATATTGCTCATAAGAAGGAGTCAGAACTCGATGACAATAATATCAAACATCTGCTTCTCGACTTGTTTCTGGCGGGAGTAGACACGAGTTCGAGTGCGGTGGAATGGGCTATGGCGGAGTTACTTCGAAACCCTAAGATGATAGTGAAAGTTCAAGAAGAGATAAGACAAGTGATAGGGCTAAAGGGCACCGTTCAAGATCTTGATATCGTTAAACTACCTTATTTACAAGCAGTTGTGAAAGAGAGTCTTCGTTTGCATCCGCCTGCTCCTTTCCTCGTCCCTCGAAAATCAGAGTCTGATGATGTCCAAATTTTCGAGTTCCTCATTCCTAAGAATACTCAGGTTCTTGTGAACGTGTGGGCAATAGGGCGAGATCCGAATGTGTGGAAGAATCCGACGCAGTTCGAGCCAGAGAGGTTTTTGGGAAGAGGAATAGATGTGAAAGGTAATCATTTTGAGCTGATTCCGTTCGGAGCTGGACGACGGATTTGTCCAGGAATGCCACTCGCGTTTAGGATAATGCATCTAGTTCTTGCGTCTCTTCTCTATGGCTTTGACTGGGAGTATCAAAACGGCGTAGTTCCTGAAAATGTGGATATGAATGAGGCCTTTGGTGCTACCTTGCACAAAGCCGAACCATTATGTATCGTGCCTATCAAGAAACGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0008150 GO:0008152 GO:0009058 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0044550 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

498

Amino Acids

56.53

Weight (kDa)

9.3

Isoelectric Point (pI)

40.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 34 - 392 3.4e-66 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1314
AccB1I GGYRCC 1 cut(s) 1010
AccB7I CCANNNNNTGG 1 cut(s) 622
AccI GTMKAC 1 cut(s) 903
AccII CGCG 1 cut(s) 1334
AccIII TCCGGA 1 cut(s) 647
AciI CCGC 5 cut(s) 324, 896, 920, 938, 1085
AclWI GGATC 1 cut(s) 1193
AcsI RAATTY 2 cut(s) 186, 1134
AfiI CCNNNNNNNGG 3 cut(s) 351, 622, 683
AflIII ACRYGT 2 cut(s) 1179, 1489
AgsI TTSAA 6 cut(s) 114, 143, 152, 755, 977, 1019
AjnI CCWGG 1 cut(s) 1318
AluBI AGCT 7 cut(s) 118, 214, 340, 506, 707, 1285, 1301
AluI AGCT 7 cut(s) 118, 214, 340, 506, 707, 1285, 1301
Alw26I GTCTC 4 cut(s) 30, 307, 490, 1368
AlwI GGATC 1 cut(s) 1193
AlwNI CAGNNNCTG 1 cut(s) 1121
Ama87I CYCGRG 1 cut(s) 519
Aor13HI TCCGGA 1 cut(s) 647
AoxI GGCC 1 cut(s) 1431
ApeKI GCWGC 1 cut(s) 489
ApoI RAATTY 2 cut(s) 186, 1134
ArsI GACNNNNNNTTYG 2 cut(s) 1296, 1328
AspS9I GGNCC 1 cut(s) 107
AsuC2I CCSGG 3 cut(s) 84, 105, 314
AsuHPI GGTGA 3 cut(s) 152, 237, 637
AsuII TTCGAA 1 cut(s) 949
AvaI CYCGRG 1 cut(s) 519
AvaII GGWCC 1 cut(s) 107
BaeGI GKGCMC 1 cut(s) 1013
BanI GGYRCC 1 cut(s) 1010
BauI CACGAG 2 cut(s) 335, 907
BbsI GAAGAC 1 cut(s) 1064
BbvI GCAGC 1 cut(s) 476
BccI CCATC 1 cut(s) 631
BceAI ACGGC 1 cut(s) 1414
BcgI CGANNNNNNTGC 2 cut(s) 439, 473
BciT130I CCWGG 1 cut(s) 1320
BclI TGATCA 1 cut(s) 280
BcnI CCSGG 3 cut(s) 84, 105, 314
BcoDI GTCTC 4 cut(s) 30, 307, 490, 1368
BfaI CTAG 3 cut(s) 425, 708, 1352
BglII AGATCT 2 cut(s) 642, 1021
BisI GCNGC 2 cut(s) 324, 490
BlsI GCNGC 2 cut(s) 325, 491
Bme1390I CCNGG 4 cut(s) 84, 105, 314, 1320
Bme18I GGWCC 1 cut(s) 107
BmeT110I CYCGRG 1 cut(s) 519
BmgT120I GGNCC 1 cut(s) 107
BmiI GGNNCC 4 cut(s) 91, 108, 401, 1012
BmrFI CCNGG 4 cut(s) 84, 105, 314, 1320
BmrI ACTGGG 1 cut(s) 1393
BmsI GCATC 4 cut(s) 533, 720, 1090, 1357
BmuI ACTGGG 1 cut(s) 1393
BpiI GAAGAC 1 cut(s) 1064
BpmI CTGGAG 1 cut(s) 289
Bpu14I TTCGAA 1 cut(s) 949
BpuEI CTTGAG 1 cut(s) 166
BpuMI CCSGG 3 cut(s) 84, 105, 314
Bsa29I ATCGAT 1 cut(s) 765
BsaBI GATNNNNATC 1 cut(s) 641
BsaWI WCCGGW 3 cut(s) 68, 247, 647
Bsc4I CCNNNNNNNGG 3 cut(s) 351, 622, 683
Bse1I ACTGG 3 cut(s) 306, 435, 1388
Bse8I GATNNNNATC 1 cut(s) 641
BseAI TCCGGA 1 cut(s) 647
BseBI CCWGG 1 cut(s) 1320
BseCI ATCGAT 1 cut(s) 765
BseGI GGATG 3 cut(s) 135, 639, 1081
BseJI GATNNNNATC 1 cut(s) 641
BseLI CCNNNNNNNGG 3 cut(s) 351, 622, 683
BseMII CTCAG 1 cut(s) 1178
BseNI ACTGG 3 cut(s) 306, 435, 1388
BseSI GKGCMC 1 cut(s) 1013
BseXI GCAGC 1 cut(s) 476
Bsh1236I CGCG 1 cut(s) 1334
BshFI GGCC 1 cut(s) 1433
BshNI GGYRCC 1 cut(s) 1010
BshVI ATCGAT 1 cut(s) 765
BsiHKCI CYCGRG 1 cut(s) 519
BsiSI CCGG 6 cut(s) 69, 84, 104, 248, 314, 648
BslFI GGGAC 2 cut(s) 66, 1088
BslI CCNNNNNNNGG 3 cut(s) 351, 622, 683
BsmAI GTCTC 4 cut(s) 30, 307, 490, 1368
BsmBI CGTCTC 2 cut(s) 490, 1368
BsmFI GGGAC 2 cut(s) 66, 1088
BsmI GAATGC 1 cut(s) 1329
BsnI GGCC 1 cut(s) 1433
BsoBI CYCGRG 1 cut(s) 519
Bsp119I TTCGAA 1 cut(s) 949
Bsp1286I GDGCHC 1 cut(s) 1013
Bsp13I TCCGGA 1 cut(s) 647
Bsp143I GATC 7 cut(s) 175, 280, 642, 694, 776, 1021, 1198
BspACI CCGC 5 cut(s) 324, 896, 920, 938, 1085
BspANI GGCC 1 cut(s) 1433
BspCNI CTCAG 1 cut(s) 1177
BspDI ATCGAT 1 cut(s) 765
BspEI TCCGGA 1 cut(s) 647
BspFNI CGCG 1 cut(s) 1334
BspHI TCATGA 1 cut(s) 468
BspLI GGNNCC 4 cut(s) 91, 108, 401, 1012
BspPI GGATC 1 cut(s) 1193
BspT104I TTCGAA 1 cut(s) 949
BspT107I GGYRCC 1 cut(s) 1010
BsrI ACTGG 3 cut(s) 306, 435, 1388
BssMI GATC 7 cut(s) 175, 280, 642, 694, 776, 1021, 1198
BssSI CACGAG 2 cut(s) 335, 907
Bst2BI CACGAG 2 cut(s) 335, 907
Bst2UI CCWGG 1 cut(s) 1320
Bst4CI ACNGT 5 cut(s) 148, 164, 232, 741, 1015
Bst6I CTCTTC 4 cut(s) 53, 975, 1246, 1371
BstAPI GCANNNNNTGC 1 cut(s) 437
BstBI TTCGAA 1 cut(s) 949
BstC8I GCNNGC 1 cut(s) 1089
BstDEI CTNAG 3 cut(s) 957, 1155, 1164
BstF5I GGATG 3 cut(s) 135, 639, 1081
BstFNI CGCG 1 cut(s) 1334
BstKTI GATC 7 cut(s) 178, 283, 645, 697, 779, 1024, 1201
BstMAI GTCTC 4 cut(s) 30, 307, 490, 1368
BstMBI GATC 7 cut(s) 175, 280, 642, 694, 776, 1021, 1198
BstMWI GCNNNNNNNGC 6 cut(s) 124, 346, 437, 730, 1231, 1439
BstNI CCWGG 1 cut(s) 1320
BstSCI CCNGG 4 cut(s) 82, 103, 312, 1318
BstSLI GKGCMC 1 cut(s) 1013
BstUI CGCG 1 cut(s) 1334
BstV1I GCAGC 1 cut(s) 476
BstV2I GAAGAC 1 cut(s) 1064
BstX2I RGATCY 3 cut(s) 642, 1021, 1198
BstYI RGATCY 3 cut(s) 642, 1021, 1198
Bsu15I ATCGAT 1 cut(s) 765
BsuRI GGCC 1 cut(s) 1433
BsuTUI ATCGAT 1 cut(s) 765
BtsCI GGATG 3 cut(s) 135, 639, 1081
BtsIMutI CAGTG 3 cut(s) 237, 313, 442
Cac8I GCNNGC 1 cut(s) 1089
CaiI CAGNNNCTG 1 cut(s) 1121
CciI TCATGA 1 cut(s) 468
Cfr13I GGNCC 1 cut(s) 107
ClaI ATCGAT 1 cut(s) 765
CseI GACGC 2 cut(s) 1231, 1350
CviAII CATG 4 cut(s) 78, 278, 284, 469
DdeI CTNAG 3 cut(s) 957, 1155, 1164
DpnI GATC 7 cut(s) 177, 282, 644, 696, 778, 1023, 1200
DpnII GATC 7 cut(s) 175, 280, 642, 694, 776, 1021, 1198
DrdI GACNNNNNNGTC 1 cut(s) 1314
DseDI GACNNNNNNGTC 1 cut(s) 1314
Eam1104I CTCTTC 4 cut(s) 53, 975, 1246, 1371
EarI CTCTTC 4 cut(s) 53, 975, 1246, 1371
EciI GGCGGA 2 cut(s) 953, 1074
Eco147I AGGCCT 1 cut(s) 1433
Eco32I GATATC 1 cut(s) 1030
Eco47I GGWCC 1 cut(s) 107
Eco88I CYCGRG 1 cut(s) 519
EcoO109I RGGNCCY 1 cut(s) 107
EcoRII CCWGG 1 cut(s) 1318
EcoRV GATATC 1 cut(s) 1030
EcoT22I ATGCAT 1 cut(s) 1350
Esp3I CGTCTC 2 cut(s) 490, 1368
FaeI CATG 4 cut(s) 81, 281, 287, 472
FalI AAGNNNNNCTT 2 cut(s) 1056, 1088
FaqI GGGAC 2 cut(s) 66, 1088
FatI CATG 4 cut(s) 77, 277, 283, 468
FauI CCCGC 1 cut(s) 889
FbaI TGATCA 1 cut(s) 280
FblI GTMKAC 1 cut(s) 903
Fnu4HI GCNGC 2 cut(s) 324, 490
FokI GGATG 3 cut(s) 122, 646, 1068
Fsp4HI GCNGC 2 cut(s) 324, 490
FspBI CTAG 3 cut(s) 425, 708, 1352
GluI GCNGC 2 cut(s) 324, 490
GsuI CTGGAG 1 cut(s) 289
HaeIII GGCC 1 cut(s) 1433
HapII CCGG 6 cut(s) 69, 84, 104, 248, 314, 648
HgaI GACGC 2 cut(s) 1231, 1350
Hin1II CATG 4 cut(s) 81, 281, 287, 472
HincII GTYRAC 1 cut(s) 481
HindII GTYRAC 1 cut(s) 481
HindIII AAGCTT 1 cut(s) 212
HpaI GTTAAC 1 cut(s) 481
HpaII CCGG 6 cut(s) 69, 84, 104, 248, 314, 648
HphI GGTGA 3 cut(s) 152, 237, 637
Hpy166II GTNNAC 5 cut(s) 167, 481, 511, 904, 1177
Hpy188I TCNGA 7 cut(s) 633, 847, 1117, 1123, 1203, 1221, 1298
Hpy8I GTNNAC 5 cut(s) 167, 481, 511, 904, 1177
Hpy99I CGWCG 2 cut(s) 1225, 1311
HpyAV CCTTC 4 cut(s) 120, 149, 448, 832
HpyCH4III ACNGT 5 cut(s) 148, 164, 232, 741, 1015
HpyCH4IV ACGT 4 cut(s) 421, 621, 1179, 1489
HpyCH4V TGCA 6 cut(s) 460, 700, 733, 1081, 1348, 1450
HpyF10VI GCNNNNNNNGC 6 cut(s) 124, 346, 437, 730, 1231, 1439
HpyF3I CTNAG 3 cut(s) 957, 1155, 1164
HpySE526I ACGT 4 cut(s) 421, 621, 1179, 1489
Hsp92II CATG 4 cut(s) 81, 281, 287, 472
Kpn2I TCCGGA 1 cut(s) 647
Ksp22I TGATCA 1 cut(s) 280
KspAI GTTAAC 1 cut(s) 481
Kzo9I GATC 7 cut(s) 175, 280, 642, 694, 776, 1021, 1198
LmnI GCTCC 4 cut(s) 89, 704, 1096, 1298
Lsp1109I GCAGC 1 cut(s) 476
LweI GCATC 4 cut(s) 533, 720, 1090, 1357
MaeI CTAG 3 cut(s) 425, 708, 1352
MaeII ACGT 4 cut(s) 421, 621, 1179, 1489
MaeIII GTNAC 3 cut(s) 131, 308, 942
MalI GATC 7 cut(s) 177, 282, 644, 696, 778, 1023, 1200
MboI GATC 7 cut(s) 175, 280, 642, 694, 776, 1021, 1198
MflI RGATCY 3 cut(s) 642, 1021, 1198
MhlI GDGCHC 1 cut(s) 1013
MluCI AATT 3 cut(s) 186, 404, 1134
MlyI GAGTC 5 cut(s) 309, 587, 851, 1078, 1127
MmeI TCCRAC 2 cut(s) 353, 1244
Mph1103I ATGCAT 1 cut(s) 1350
MroI TCCGGA 1 cut(s) 647
MseI TTAA 3 cut(s) 210, 480, 1035
MslI CAYNNNNRTG 1 cut(s) 282
MspA1I CMGCKG 1 cut(s) 326
MspI CCGG 6 cut(s) 69, 84, 104, 248, 314, 648
MspR9I CCNGG 4 cut(s) 84, 105, 314, 1320
Mva1269I GAATGC 1 cut(s) 1329
MvaI CCWGG 1 cut(s) 1320
MvnI CGCG 1 cut(s) 1334
MwoI GCNNNNNNNGC 6 cut(s) 124, 346, 437, 730, 1231, 1439
NciI CCSGG 3 cut(s) 84, 105, 314
NdeII GATC 7 cut(s) 175, 280, 642, 694, 776, 1021, 1198
NlaIII CATG 4 cut(s) 81, 281, 287, 472
NlaIV GGNNCC 4 cut(s) 91, 108, 401, 1012
NmuCI GTSAC 1 cut(s) 308
NsiI ATGCAT 1 cut(s) 1350
NspV TTCGAA 1 cut(s) 949
PaeR7I CTCGAG 1 cut(s) 519
PagI TCATGA 1 cut(s) 468
PceI AGGCCT 1 cut(s) 1433
PctI GAATGC 1 cut(s) 1329
PfeI GAWTC 6 cut(s) 355, 628, 759, 812, 1216, 1288
PflMI CCANNNNNTGG 1 cut(s) 622
PfoI TCCNGGA 1 cut(s) 1318
PkrI GCNGC 2 cut(s) 325, 491
PleI GAGTC 5 cut(s) 308, 587, 850, 1077, 1126
PpsI GAGTC 5 cut(s) 308, 587, 850, 1077, 1126
PpuMI RGGWCCY 1 cut(s) 107
Psp5II RGGWCCY 1 cut(s) 107
Psp6I CCWGG 1 cut(s) 1318
PspGI CCWGG 1 cut(s) 1318
PspN4I GGNNCC 4 cut(s) 91, 108, 401, 1012
PspPI GGNCC 1 cut(s) 107
PspPPI RGGWCCY 1 cut(s) 107
PstNI CAGNNNCTG 1 cut(s) 1121
PsuI RGATCY 3 cut(s) 642, 1021, 1198
RseI CAYNNNNRTG 1 cut(s) 282
SaqAI TTAA 3 cut(s) 210, 480, 1035
SatI GCNGC 2 cut(s) 324, 490
Sau3AI GATC 7 cut(s) 175, 280, 642, 694, 776, 1021, 1198
Sau96I GGNCC 1 cut(s) 107
SchI GAGTC 5 cut(s) 309, 587, 851, 1078, 1127
ScrFI CCNGG 4 cut(s) 84, 105, 314, 1320
SduI GDGCHC 1 cut(s) 1013
SfaNI GCATC 4 cut(s) 533, 720, 1090, 1357
Sfr274I CTCGAG 1 cut(s) 519
SfuI TTCGAA 1 cut(s) 949
SinI GGWCC 1 cut(s) 107
SlaI CTCGAG 1 cut(s) 519
SmiMI CAYNNNNRTG 1 cut(s) 282
SmlI CTYRAG 2 cut(s) 181, 519
SmoI CTYRAG 2 cut(s) 181, 519
Sse9I AATT 3 cut(s) 186, 404, 1134
SseBI AGGCCT 1 cut(s) 1433
SsiI CCGC 5 cut(s) 324, 896, 920, 938, 1085
SspMI CTAG 3 cut(s) 425, 708, 1352
StuI AGGCCT 1 cut(s) 1433
StyD4I CCNGG 4 cut(s) 82, 103, 312, 1318
TaaI ACNGT 5 cut(s) 148, 164, 232, 741, 1015
TaiI ACGT 4 cut(s) 424, 624, 1182, 1492
TasI AATT 3 cut(s) 186, 404, 1134
TauI GCSGC 1 cut(s) 326
TfiI GAWTC 6 cut(s) 355, 628, 759, 812, 1216, 1288
Tru1I TTAA 3 cut(s) 210, 480, 1035
Tru9I TTAA 3 cut(s) 210, 480, 1035
TscAI CASTG 3 cut(s) 237, 313, 442
TseFI GTSAC 1 cut(s) 308
TseI GCWGC 1 cut(s) 489
Tsp45I GTSAC 1 cut(s) 308
TspDTI ATGAA 4 cut(s) 485, 520, 751, 1439
TspGWI ACGGA 4 cut(s) 207, 351, 1281, 1324
TspRI CASTG 3 cut(s) 237, 313, 442
Van91I CCANNNNNTGG 1 cut(s) 622
VpaK11BI GGWCC 1 cut(s) 107
XapI RAATTY 2 cut(s) 186, 1134
XbaI TCTAGA 1 cut(s) 424
XhoI CTCGAG 1 cut(s) 519
XmiI GTMKAC 1 cut(s) 903
XspI CTAG 3 cut(s) 425, 708, 1352
Zsp2I ATGCAT 1 cut(s) 1350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.