AT2G45570

cytochrome P450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001080
Physical Location & Seq
Reverse (-)
18779321 .. 18781973
2653 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G45570.1

Sequence Viewer

Length: 1539 bp
ATGGATATCATCTTTGAACAAGCTTTGTTCCCTCTATTTTGCTTTGTCTTATCATTTTTCATAATCTTCTTCACTACAACAAGACCCCGGAGCTCTCGCAAGGTCGTTCCATCTCCGCCGGGACCTCCGCGACTGCCCATCATCGGAAACATTCACCTTGTCGGTAGAAACCCACACCATTCATTCGCTGACCTCTCAAAAACATATGGACCAATCATGAGCCTTAAATTTGGAAGTTTAAACACAGTGGTCGTAACTTCACCTGAGGCTGCAAGAGAGGTTCTAAGAACATACGACCAAATCCTCTCTAGCCGTACCCCCACTAACTCGATACGGTCCATTAATCACGACAAGGTTTCCGTTGTCTGGCTTCCACCATCGTCGTCTCGTTGGAGACTTTTGAGAAAACTGTCGGCGACTCAGCTCTTCTCGCCTCAGCGTATCGAAGCCACGAAAACGTTGAGGGAGAACAAGGTGAAGGAACTCGTGAGCTTCATGAGTGAAAGCAGCGAGAGAGAAGAAGCTGTTGATATATCTCGTGCTACCTTCATCACTGCTCTTAATATCATATCAAACATTCTGTTTTCTGTCGATCTCGGTAACTACGACTCGAATAAATCCGGTGTGTTTCAAGACACGGTGATTGGTGTCATGGAAGCTGTCGGGAATCCAGACGCTGCTAACTTCTTTCCATTTCTGGGGTTCCTTGATCTGCAAGGTAATAGAAAGACTCTGAAGGCTTGTTCCGAGAGATTGTTTAAGGTTTTCCGTGGGTTCATTGATGCTAAATTAGCGGAAAAATCATTGCGGGATACTAACTCTAAAGATGTCCGGGAGAGAGATTTTGTGGATGTGCTTCTCGATCTTACCGAAGGAGATGAAGCAGAGCTCAACACTAACGATATTGTACACCTTCTCTTGGACCTGTTTGGAGCGGGCACAGACACAAACTCTAGTACCGTGGAATGGGCAATGGCAGAGTTACTTCGAAACCCTGAAACAATGGTGAAAGCTCAGGCTGAGATCGACTGTGTGATTGGTCAGAAAGGTGTTGTTGAAGAGTCTGATATCTCGGCACTTCCCTATCTACAAGCAGTGGTGAAGGAAACTTTCCGGTTACATCCGGCTGCTCCGCTACTAGTCCCCCGAAAAGCTGAATCAGATGTGGAGGTTCTTGGGTTCATGGTGCCTAAAGACACTCAGGTATTTGTGAACGTGTGGGCCATAGGAAGAGACCCGAACGTGTGGGAGAATTCGTCCCGGTTTAAGCCAGAGAGGTTTTTAGGGAAAGATATCGACCTGAGAGGTAGAGATTATGAGCTTACACCATTCGGAGCCGGACGTAGAATATGCCCGGGATTGCCTTTGGCTGTGAAAACAGTGCCTCTTATGCTTGCTTCTCTTCTTTATTCATTTGACTGGAAGCTTCCAAACGGTGTCGGTTCAGAGGATTTGGACATGGACGAGACTTTCGGTCTCACATTGCACAAGACCAACCCGTTACATGCCGTACCCGTCAAGAAACGTGGCCGTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0008150 GO:0008152 GO:0009058 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0044550 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

512

Amino Acids

57.26

Weight (kDa)

6.5

Isoelectric Point (pI)

40.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 39 - 499 2.2e-108 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1186
AccBSI CCGCTC 1 cut(s) 935
AccII CGCG 1 cut(s) 130
AciI CCGC 6 cut(s) 116, 128, 794, 808, 935, 1133
AclI AACGTT 1 cut(s) 458
AcoI YGGCCR 1 cut(s) 1528
AcsI RAATTY 2 cut(s) 227, 1252
AcuI CTGAAG 1 cut(s) 755
AfaI GTAC 4 cut(s) 316, 909, 958, 1512
AfiI CCNNNNNNNGG 5 cut(s) 143, 366, 698, 919, 966
AflIII ACRYGT 2 cut(s) 1215, 1242
AgsI TTSAA 3 cut(s) 17, 632, 1058
AhdI GACNNNNNGTC 1 cut(s) 1473
AhlI ACTAGT 1 cut(s) 1138
Alw21I GWGCWC 2 cut(s) 95, 891
Alw26I GTCTC 5 cut(s) 388, 390, 1227, 1460, 1481
AlwNI CAGNNNCTG 1 cut(s) 677
Ama87I CYCGRG 1 cut(s) 1354
AoxI GGCC 2 cut(s) 1221, 1528
ApeKI GCWGC 4 cut(s) 269, 507, 677, 1127
ApoI RAATTY 2 cut(s) 227, 1252
ArsI GACNNNNNNTTYG 2 cut(s) 1454, 1486
AseI ATTAAT 1 cut(s) 342
AspS9I GGNCC 5 cut(s) 122, 209, 336, 922, 1221
AsuC2I CCSGG 6 cut(s) 88, 120, 833, 1261, 1355, 1356
AsuHPI GGTGA 6 cut(s) 146, 252, 487, 652, 1018, 1111
AsuII TTCGAA 1 cut(s) 988
AvaI CYCGRG 1 cut(s) 1354
AvaII GGWCC 4 cut(s) 122, 209, 336, 922
AxyI CCTNAGG 1 cut(s) 264
BaeGI GKGCMC 1 cut(s) 941
BaeI ACNNNNGTAYC 2 cut(s) 940, 973
BanI GGYRCC 1 cut(s) 1186
BanII GRGCYC 2 cut(s) 95, 891
BauI CACGAG 2 cut(s) 485, 537
Bbv12I GWGCWC 2 cut(s) 95, 891
BbvCI CCTCAGC 1 cut(s) 435
BbvI GCAGC 4 cut(s) 256, 519, 664, 1114
BccI CCATC 3 cut(s) 118, 146, 385
BceAI ACGGC 3 cut(s) 297, 1493, 1515
BciVI GTATCC 1 cut(s) 805
BcnI CCSGG 6 cut(s) 88, 120, 833, 1261, 1355, 1356
BcoDI GTCTC 5 cut(s) 388, 390, 1227, 1460, 1481
BcuI ACTAGT 1 cut(s) 1138
BfaI CTAG 3 cut(s) 309, 954, 1139
BfuI GTATCC 1 cut(s) 805
BisI GCNGC 4 cut(s) 270, 508, 678, 1128
BlsI GCNGC 4 cut(s) 271, 509, 679, 1129
Bme1390I CCNGG 6 cut(s) 88, 120, 833, 1261, 1355, 1356
Bme18I GGWCC 4 cut(s) 122, 209, 336, 922
BmeRI GACNNNNNGTC 1 cut(s) 1473
BmeT110I CYCGRG 1 cut(s) 1354
BmgT120I GGNCC 5 cut(s) 122, 209, 336, 922, 1221
BmiI GGNNCC 4 cut(s) 123, 704, 1188, 1336
BmrFI CCNGG 6 cut(s) 88, 120, 833, 1261, 1355, 1356
BmsI GCATC 1 cut(s) 772
Bpu10I CCTNAGC 2 cut(s) 435, 1014
Bpu14I TTCGAA 1 cut(s) 988
BpuMI CCSGG 6 cut(s) 88, 120, 833, 1261, 1355, 1356
BsaI GGTCTC 2 cut(s) 1227, 1481
BsaJI CCNNGG 4 cut(s) 86, 769, 960, 1354
BsaWI WCCGGW 2 cut(s) 620, 1113
BsaXI ACNNNNNCTCC 2 cut(s) 1241, 1271
Bsc4I CCNNNNNNNGG 5 cut(s) 143, 366, 698, 919, 966
Bse1I ACTGG 1 cut(s) 1424
Bse21I CCTNAGG 1 cut(s) 264
Bse3DI GCAATG 3 cut(s) 803, 978, 1481
BseDI CCNNGG 4 cut(s) 86, 769, 960, 1354
BseGI GGATG 2 cut(s) 856, 1120
BseLI CCNNNNNNNGG 5 cut(s) 143, 366, 698, 919, 966
BseMI GCAATG 3 cut(s) 803, 978, 1481
BseMII CTCAG 7 cut(s) 255, 434, 449, 1011, 1028, 1214, 1292
BseNI ACTGG 1 cut(s) 1424
BseSI GKGCMC 1 cut(s) 941
BseXI GCAGC 4 cut(s) 256, 519, 664, 1114
Bsh1236I CGCG 1 cut(s) 130
BshFI GGCC 2 cut(s) 1223, 1530
BshNI GGYRCC 1 cut(s) 1186
BsiHKAI GWGCWC 2 cut(s) 95, 891
BsiHKCI CYCGRG 1 cut(s) 1354
BsiSI CCGG 9 cut(s) 88, 119, 621, 832, 1114, 1124, 1261, 1338, 1355
BslFI GGGAC 3 cut(s) 135, 1127, 1243
BslI CCNNNNNNNGG 5 cut(s) 143, 366, 698, 919, 966
BsmAI GTCTC 5 cut(s) 388, 390, 1227, 1460, 1481
BsmBI CGTCTC 1 cut(s) 390
BsmFI GGGAC 3 cut(s) 135, 1127, 1243
BsnI GGCC 2 cut(s) 1223, 1530
Bso31I GGTCTC 2 cut(s) 1227, 1481
BsoBI CYCGRG 1 cut(s) 1354
Bsp119I TTCGAA 1 cut(s) 988
Bsp1286I GDGCHC 3 cut(s) 95, 891, 941
Bsp1407I TGTACA 1 cut(s) 907
Bsp143I GATC 4 cut(s) 592, 709, 862, 1023
BspACI CCGC 6 cut(s) 116, 128, 794, 808, 935, 1133
BspANI GGCC 2 cut(s) 1223, 1530
BspCNI CTCAG 7 cut(s) 256, 433, 448, 1012, 1027, 1213, 1293
BspFNI CGCG 1 cut(s) 130
BspHI TCATGA 2 cut(s) 216, 495
BspLI GGNNCC 4 cut(s) 123, 704, 1188, 1336
BspQI GCTCTTC 1 cut(s) 431
BspT104I TTCGAA 1 cut(s) 988
BspT107I GGYRCC 1 cut(s) 1186
BspTNI GGTCTC 2 cut(s) 1227, 1481
BsrBI CCGCTC 1 cut(s) 935
BsrDI GCAATG 3 cut(s) 803, 978, 1481
BsrGI TGTACA 1 cut(s) 907
BsrI ACTGG 1 cut(s) 1424
BssECI CCNNGG 4 cut(s) 86, 769, 960, 1354
BssMI GATC 4 cut(s) 592, 709, 862, 1023
BssSI CACGAG 2 cut(s) 485, 537
Bst2BI CACGAG 2 cut(s) 485, 537
Bst4CI ACNGT 8 cut(s) 247, 336, 411, 640, 961, 1031, 1381, 1436
Bst6I CTCTTC 4 cut(s) 431, 1053, 1225, 1407
BstAUI TGTACA 1 cut(s) 907
BstBI TTCGAA 1 cut(s) 988
BstC8I GCNNGC 2 cut(s) 937, 1395
BstDEI CTNAG 8 cut(s) 264, 284, 420, 435, 1014, 1020, 1200, 1301
BstDSI CCRYGG 2 cut(s) 769, 960
BstF5I GGATG 2 cut(s) 856, 1120
BstFNI CGCG 1 cut(s) 130
BstKTI GATC 4 cut(s) 595, 712, 865, 1026
BstMAI GTCTC 5 cut(s) 388, 390, 1227, 1460, 1481
BstMBI GATC 4 cut(s) 592, 709, 862, 1023
BstMWI GCNNNNNNNGC 3 cut(s) 430, 791, 1390
BstNSI RCATGY 1 cut(s) 1508
BstSCI CCNGG 6 cut(s) 86, 118, 831, 1259, 1353, 1354
BstSLI GKGCMC 1 cut(s) 941
BstUI CGCG 1 cut(s) 130
BstV1I GCAGC 4 cut(s) 256, 519, 664, 1114
Bsu36I CCTNAGG 1 cut(s) 264
BsuI GTATCC 1 cut(s) 805
BsuRI GGCC 2 cut(s) 1223, 1530
BtgI CCRYGG 2 cut(s) 769, 960
BtsCI GGATG 2 cut(s) 856, 1120
BtsI GCAGTG 2 cut(s) 552, 1101
BtsIMutI CAGTG 4 cut(s) 252, 552, 1101, 1386
Cac8I GCNNGC 2 cut(s) 937, 1395
CaiI CAGNNNCTG 1 cut(s) 677
CciI TCATGA 2 cut(s) 216, 495
Cfr13I GGNCC 5 cut(s) 122, 209, 336, 922, 1221
Cfr9I CCCGGG 1 cut(s) 1354
CseI GACGC 1 cut(s) 683
Csp6I GTAC 4 cut(s) 315, 908, 957, 1511
CspCI CAANNNNNGTGG 1 cut(s) 1507
CviAII CATG 6 cut(s) 217, 496, 652, 1183, 1459, 1505
CviQI GTAC 4 cut(s) 315, 908, 957, 1511
DdeI CTNAG 8 cut(s) 264, 284, 420, 435, 1014, 1020, 1200, 1301
DpnI GATC 4 cut(s) 594, 711, 864, 1025
DpnII GATC 4 cut(s) 592, 709, 862, 1023
DraI TTTAAA 1 cut(s) 240
DriI GACNNNNNGTC 1 cut(s) 1473
EaeI YGGCCR 1 cut(s) 1528
Eam1104I CTCTTC 4 cut(s) 431, 1053, 1225, 1407
Eam1105I GACNNNNNGTC 1 cut(s) 1473
EarI CTCTTC 4 cut(s) 431, 1053, 1225, 1407
EciI GGCGGA 1 cut(s) 105
Ecl136II GAGCTC 2 cut(s) 93, 889
Eco24I GRGCYC 2 cut(s) 95, 891
Eco31I GGTCTC 2 cut(s) 1227, 1481
Eco32I GATATC 3 cut(s) 7, 1069, 1294
Eco47I GGWCC 4 cut(s) 122, 209, 336, 922
Eco53kI GAGCTC 2 cut(s) 93, 889
Eco57I CTGAAG 1 cut(s) 755
Eco81I CCTNAGG 1 cut(s) 264
Eco88I CYCGRG 1 cut(s) 1354
EcoICRI GAGCTC 2 cut(s) 93, 889
EcoO109I RGGNCCY 1 cut(s) 122
EcoRI GAATTC 1 cut(s) 1252
EcoRV GATATC 3 cut(s) 7, 1069, 1294
EcoT38I GRGCYC 2 cut(s) 95, 891
Esp3I CGTCTC 1 cut(s) 390
FaeI CATG 6 cut(s) 220, 499, 655, 1186, 1462, 1508
FaqI GGGAC 3 cut(s) 135, 1127, 1243
FatI CATG 6 cut(s) 216, 495, 651, 1182, 1458, 1504
FauI CCCGC 2 cut(s) 801, 928
FauNDI CATATG 1 cut(s) 205
Fnu4HI GCNGC 4 cut(s) 270, 508, 678, 1128
FokI GGATG 2 cut(s) 863, 1107
FriOI GRGCYC 2 cut(s) 95, 891
Fsp4HI GCNGC 4 cut(s) 270, 508, 678, 1128
FspBI CTAG 3 cut(s) 309, 954, 1139
GluI GCNGC 4 cut(s) 270, 508, 678, 1128
HaeIII GGCC 2 cut(s) 1223, 1530
HapII CCGG 9 cut(s) 88, 119, 621, 832, 1114, 1124, 1261, 1338, 1355
HgaI GACGC 1 cut(s) 683
Hin1II CATG 6 cut(s) 220, 499, 655, 1186, 1462, 1508
HindIII AAGCTT 2 cut(s) 21, 1424
HinfI GANTC 6 cut(s) 418, 608, 667, 730, 1061, 1157
HpaII CCGG 9 cut(s) 88, 119, 621, 832, 1114, 1124, 1261, 1338, 1355
HphI GGTGA 6 cut(s) 146, 252, 487, 652, 1018, 1111
Hpy166II GTNNAC 2 cut(s) 910, 1213
Hpy188I TCNGA 8 cut(s) 146, 735, 748, 1044, 1066, 1162, 1334, 1447
Hpy188III TCNNGA 9 cut(s) 217, 347, 487, 496, 632, 664, 671, 860, 1519
Hpy8I GTNNAC 2 cut(s) 910, 1213
Hpy99I CGWCG 1 cut(s) 385
HpyAV CCTTC 6 cut(s) 472, 556, 730, 866, 923, 1096
HpyCH4III ACNGT 8 cut(s) 247, 336, 411, 640, 961, 1031, 1381, 1436
HpyCH4IV ACGT 5 cut(s) 458, 1215, 1242, 1342, 1525
HpyCH4V TGCA 3 cut(s) 272, 715, 1486
HpyF10VI GCNNNNNNNGC 3 cut(s) 430, 791, 1390
HpyF3I CTNAG 8 cut(s) 264, 284, 420, 435, 1014, 1020, 1200, 1301
HpySE526I ACGT 5 cut(s) 458, 1215, 1242, 1342, 1525
Hsp92II CATG 6 cut(s) 220, 499, 655, 1186, 1462, 1508
Kzo9I GATC 4 cut(s) 592, 709, 862, 1023
LguI GCTCTTC 1 cut(s) 431
LmnI GCTCC 4 cut(s) 90, 932, 1135, 1334
Lsp1109I GCAGC 4 cut(s) 256, 519, 664, 1114
LweI GCATC 1 cut(s) 772
MaeI CTAG 3 cut(s) 309, 954, 1139
MaeII ACGT 5 cut(s) 458, 1215, 1242, 1342, 1525
MaeIII GTNAC 5 cut(s) 253, 599, 981, 1116, 1500
MalI GATC 4 cut(s) 594, 711, 864, 1025
MbiI CCGCTC 1 cut(s) 935
MboI GATC 4 cut(s) 592, 709, 862, 1023
MboII GAAGA 7 cut(s) 58, 61, 418, 530, 1070, 1242, 1394
MhlI GDGCHC 3 cut(s) 95, 891, 941
MluCI AATT 4 cut(s) 227, 788, 1252, 1534
MlyI GAGTC 4 cut(s) 412, 602, 724, 1070
MmeI TCCRAC 1 cut(s) 371
MseI TTAA 6 cut(s) 225, 239, 342, 561, 759, 1266
MspI CCGG 9 cut(s) 88, 119, 621, 832, 1114, 1124, 1261, 1338, 1355
MspR9I CCNGG 6 cut(s) 88, 120, 833, 1261, 1355, 1356
MssI GTTTAAAC 1 cut(s) 240
MvnI CGCG 1 cut(s) 130
MwoI GCNNNNNNNGC 3 cut(s) 430, 791, 1390
NciI CCSGG 6 cut(s) 88, 120, 833, 1261, 1355, 1356
NdeI CATATG 1 cut(s) 205
NdeII GATC 4 cut(s) 592, 709, 862, 1023
NlaIII CATG 6 cut(s) 220, 499, 655, 1186, 1462, 1508
NlaIV GGNNCC 4 cut(s) 123, 704, 1188, 1336
NmeAIII GCCGAG 1 cut(s) 1052
NspI RCATGY 1 cut(s) 1508
NspV TTCGAA 1 cut(s) 988
PagI TCATGA 2 cut(s) 216, 495
PciSI GCTCTTC 1 cut(s) 431
PcsI WCGNNNNNNNCGW 2 cut(s) 603, 867
PfeI GAWTC 2 cut(s) 667, 1157
PfoI TCCNGGA 1 cut(s) 831
PkrI GCNGC 4 cut(s) 271, 509, 679, 1129
PleI GAGTC 4 cut(s) 412, 602, 724, 1069
PmeI GTTTAAAC 1 cut(s) 240
PpsI GAGTC 4 cut(s) 412, 602, 724, 1069
PpuMI RGGWCCY 1 cut(s) 122
PshBI ATTAAT 1 cut(s) 342
Psp124BI GAGCTC 2 cut(s) 95, 891
Psp1406I AACGTT 1 cut(s) 458
Psp5II RGGWCCY 1 cut(s) 122
PspN4I GGNNCC 4 cut(s) 123, 704, 1188, 1336
PspPI GGNCC 5 cut(s) 122, 209, 336, 922, 1221
PspPPI RGGWCCY 1 cut(s) 122
PstNI CAGNNNCTG 1 cut(s) 677
RsaI GTAC 4 cut(s) 316, 909, 958, 1512
RsaNI GTAC 4 cut(s) 315, 908, 957, 1511
SacI GAGCTC 2 cut(s) 95, 891
SapI GCTCTTC 1 cut(s) 431
SaqAI TTAA 6 cut(s) 225, 239, 342, 561, 759, 1266
SatI GCNGC 4 cut(s) 270, 508, 678, 1128
Sau3AI GATC 4 cut(s) 592, 709, 862, 1023
Sau96I GGNCC 5 cut(s) 122, 209, 336, 922, 1221
SchI GAGTC 4 cut(s) 412, 602, 724, 1070
ScrFI CCNGG 6 cut(s) 88, 120, 833, 1261, 1355, 1356
SduI GDGCHC 3 cut(s) 95, 891, 941
SfaNI GCATC 1 cut(s) 772
SfuI TTCGAA 1 cut(s) 988
SinI GGWCC 4 cut(s) 122, 209, 336, 922
SmaI CCCGGG 1 cut(s) 1356
SpeI ACTAGT 1 cut(s) 1138
Sse9I AATT 4 cut(s) 227, 788, 1252, 1534
SsiI CCGC 6 cut(s) 116, 128, 794, 808, 935, 1133
SspMI CTAG 3 cut(s) 309, 954, 1139
SstI GAGCTC 2 cut(s) 95, 891
StyD4I CCNGG 6 cut(s) 86, 118, 831, 1259, 1353, 1354
TaaI ACNGT 8 cut(s) 247, 336, 411, 640, 961, 1031, 1381, 1436
TaiI ACGT 5 cut(s) 461, 1218, 1245, 1345, 1528
TaqI TCGA 8 cut(s) 329, 444, 591, 611, 861, 988, 1026, 1296
TaqII GACCGA 1 cut(s) 1463
TasI AATT 4 cut(s) 227, 788, 1252, 1534
TatI WGTACW 1 cut(s) 907
TfiI GAWTC 2 cut(s) 667, 1157
Tru1I TTAA 6 cut(s) 225, 239, 342, 561, 759, 1266
Tru9I TTAA 6 cut(s) 225, 239, 342, 561, 759, 1266
TscAI CASTG 4 cut(s) 252, 559, 1101, 1386
TseI GCWGC 4 cut(s) 269, 507, 677, 1127
TspDTI ATGAA 8 cut(s) 49, 171, 484, 538, 766, 894, 1171, 1401
TspGWI ACGGA 2 cut(s) 349, 758
TspMI CCCGGG 1 cut(s) 1354
TspRI CASTG 4 cut(s) 252, 559, 1101, 1386
VpaK11BI GGWCC 4 cut(s) 122, 209, 336, 922
VspI ATTAAT 1 cut(s) 342
XapI RAATTY 2 cut(s) 227, 1252
XceI RCATGY 1 cut(s) 1508
XmaI CCCGGG 1 cut(s) 1354
XspI CTAG 3 cut(s) 309, 954, 1139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.