RLG00000003258

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001080
Physical Location & Seq
Forward (+)
46211912 .. 46213785
1874 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003258

Sequence Viewer

Length: 1515 bp
ATGAACTTTTGGAGTGCTTTCGTTTACCTTGTCCTCACCTGGACATTTATCCAAGCCCTCCTCTACTTAATTTCAAGAGCCAAAAACAGCTCCAAAAAGCTTCCACCAGGTCCAAAACCGCTTCCAATCCTCGGAAACCTTCTCCTACTTGGTGACAAACCTCACAAATCTCTTGCCGAACTTGCCAAAATCCATGGCCCTTTGATGTCCCTAAAACTTGGACAAGTAACCTCCATAGTTGTTTCTTCATCAGCCATGGCTAAACAAGTCCTCCAAACCCATGACCAATACTTGTCCAATCGAACAGTCCCTGATGCCATTCGAGCCCTTGATCACCACACTGTTGGCTTGCCATGGATCCCAGTCTCTCCCCTTTGGAGAAACCAAAGAAAAATATGCAACACTCAGCTATTTGCCAACAAAATGCTTGATGCTAACCAAAACTTGCGACGGAAGAAAGTGGAGCAACTCCTTGAATTAGTCCATCAAAGCAGCCTGAGTGGGGAAGCAGTTGACTTTGGTAGGGCAGCTTTTACTACGACACTCAATTTGCTGTCTAATACAATTCTTTCGATTGATTTAGCAGACCCGAGTTCCAAAATGGCCAGGGAGTTCAAGGAGATGGTGCATAATATTATGATCGAGGCTGGAAAACCAAACTTGGGGGACTGTTTTCCTGTGCTTAGGAAGATTGATCCTAATGGCAGAAGGAGGAGAATGACAAAGTATTTTGGAAAGATAATAGATTTAGTTGAAACAATAGTTCAGCAGAGGTTGGAGCTGAGAAAATCGACTGGATCTGCAACAAAGAACGATGTGTTGGATACCCTTCTCACTATTACAGAGGAAAACAGTGAGGAGCTCAACAAAAATCAACTTTATCATTTCATCCTAGTCAGGTCAGACACAGCTTCATCCACATTCCAATGGGCAATGGCAGAGCTCTTGCACAACCCTGAAGTTCTCTCAAAAGCACAATTAGAGCTTAACCAAGTAATTGGCAAAGGCAATCCTGTTGAGGAATCAGACATTACTAGACTACCTTACTTGCAAGCCATTATTAAAGAAACATTTCGTATGCACCCTGCAGTTCCTTTGTTACTTCCCCGAAAAGCCGAATCAGATGTAGAGATTGAAGGCTCCACAGTCCCGAAGGGTGCACAAGTGCTTGTTAACACATGGGCCATTGGCAGAGACCCAAGCATATGGGATGACCCGAATTCATTCAAGCCGGAGAGGTTCTTGGGGTCTCTACTCGATGTTAGAGGCCGGAATTTTGAACTTATTCCATTTGGCGCCGGCAGGAGAATATGTCCGGGATTGCCATTGGCAATGAGAATGTTGCACTTAATGTTAGGTTCTCTTATCCATTCCTTTGTTTGGAAGCTTGAAGATGGAGTTACACCAGAGAACATGAACATGGATGATAAGTTTGTACTCACCCTAGGGATGGCCAAACCCTTGAGAGCTATCCCTATCCCTATCCCTATCCCTATCCCTATCCCTATCCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0008150 GO:0008152 GO:0009058 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0019748 GO:0044550 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
p450

Protein Analysis

505

Amino Acids

56.54

Weight (kDa)

9.36

Isoelectric Point (pI)

36.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 35 - 480 7.7e-98 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1295
AciI CCGC 1 cut(s) 119
AclWI GGATC 4 cut(s) 352, 365, 689, 805
AcoI YGGCCR 2 cut(s) 603, 1452
AcsI RAATTY 2 cut(s) 1219, 1273
AcuI CTGAAG 1 cut(s) 978
AcyI GRCGYC 1 cut(s) 1296
AfaI GTAC 1 cut(s) 1437
AfiI CCNNNNNNNGG 5 cut(s) 131, 502, 662, 1380, 1450
AgsI TTSAA 8 cut(s) 75, 476, 616, 755, 1136, 1228, 1280, 1391
AjnI CCWGG 3 cut(s) 38, 106, 605
AjuI GAANNNNNNNTTGG 2 cut(s) 803, 835
Alw21I GWGCWC 3 cut(s) 864, 945, 1162
Alw26I GTCTC 3 cut(s) 370, 1188, 1254
Alw44I GTGCAC 1 cut(s) 1158
AlwI GGATC 4 cut(s) 352, 365, 689, 805
AlwNI CAGNNNCTG 1 cut(s) 311
Ama87I CYCGRG 1 cut(s) 589
AoxI GGCC 5 cut(s) 196, 603, 1182, 1267, 1452
ApaLI GTGCAC 1 cut(s) 1158
ApeKI GCWGC 2 cut(s) 492, 527
ApoI RAATTY 2 cut(s) 1219, 1273
ArsI GACNNNNNNTTYG 2 cut(s) 532, 564
Asp700I GAANNNNTTC 3 cut(s) 1071, 1223, 1284
AspA2I CCTAGG 1 cut(s) 1444
AspLEI GCGC 1 cut(s) 1298
AspS9I GGNCC 3 cut(s) 110, 197, 1182
AsuC2I CCSGG 1 cut(s) 1317
AsuHPI GGTGA 4 cut(s) 28, 164, 326, 1432
AvaI CYCGRG 1 cut(s) 589
AvaII GGWCC 1 cut(s) 110
AvrII CCTAGG 1 cut(s) 1444
BaeGI GKGCMC 1 cut(s) 1162
BalI TGGCCA 2 cut(s) 605, 1454
BamHI GGATCC 1 cut(s) 357
BanI GGYRCC 1 cut(s) 1295
BanII GRGCYC 3 cut(s) 328, 864, 945
Bbv12I GWGCWC 3 cut(s) 864, 945, 1162
BbvI GCAGC 2 cut(s) 504, 539
BccI CCATC 4 cut(s) 492, 616, 1388, 1444
BciT130I CCWGG 3 cut(s) 40, 108, 607
BciVI GTATCC 1 cut(s) 817
BclI TGATCA 1 cut(s) 331
BcnI CCSGG 1 cut(s) 1317
BcoDI GTCTC 3 cut(s) 370, 1188, 1254
BfaI CTAG 3 cut(s) 893, 1035, 1445
BfmI CTRYAG 1 cut(s) 1086
BfoI RGCGCY 1 cut(s) 1299
BfuI GTATCC 1 cut(s) 817
BisI GCNGC 2 cut(s) 493, 528
BlnI CCTAGG 1 cut(s) 1444
BlsI GCNGC 2 cut(s) 494, 529
Bme1390I CCNGG 4 cut(s) 40, 108, 607, 1317
Bme18I GGWCC 1 cut(s) 110
BmeT110I CYCGRG 1 cut(s) 589
BmgT120I GGNCC 3 cut(s) 110, 197, 1182
BmiI GGNNCC 3 cut(s) 359, 1141, 1297
BmrFI CCNGG 4 cut(s) 40, 108, 607, 1317
BmrI ACTGGG 1 cut(s) 356
BmsI GCATC 2 cut(s) 304, 421
BmuI ACTGGG 1 cut(s) 356
Bpu10I CCTNAGC 1 cut(s) 683
BpuEI CTTGAG 1 cut(s) 1483
BpuMI CCSGG 1 cut(s) 1317
BsaHI GRCGYC 1 cut(s) 1296
BsaI GGTCTC 2 cut(s) 1188, 1254
BsaJI CCNNGG 6 cut(s) 130, 193, 255, 353, 606, 1444
BsaXI ACNNNNNCTCC 4 cut(s) 255, 285, 1297, 1327
Bsc4I CCNNNNNNNGG 5 cut(s) 131, 502, 662, 1380, 1450
Bse118I RCCGGY 1 cut(s) 1298
Bse1I ACTGG 2 cut(s) 362, 799
Bse3DI GCAATG 2 cut(s) 939, 1338
BseBI CCWGG 3 cut(s) 40, 108, 607
BseDI CCNNGG 6 cut(s) 130, 193, 255, 353, 606, 1444
BseGI GGATG 5 cut(s) 888, 914, 1216, 1429, 1455
BseLI CCNNNNNNNGG 5 cut(s) 131, 502, 662, 1380, 1450
BseMI GCAATG 2 cut(s) 939, 1338
BseMII CTCAG 3 cut(s) 419, 488, 773
BseNI ACTGG 2 cut(s) 362, 799
BseRI GAGGAG 3 cut(s) 50, 727, 872
BseSI GKGCMC 1 cut(s) 1162
BseXI GCAGC 2 cut(s) 504, 539
BshFI GGCC 5 cut(s) 198, 605, 1184, 1269, 1454
BshNI GGYRCC 1 cut(s) 1295
BsiHKAI GWGCWC 3 cut(s) 864, 945, 1162
BsiHKCI CYCGRG 1 cut(s) 589
BsiSI CCGG 4 cut(s) 1232, 1270, 1299, 1316
BslFI GGGAC 4 cut(s) 193, 293, 680, 1133
BslI CCNNNNNNNGG 5 cut(s) 131, 502, 662, 1380, 1450
BsmAI GTCTC 3 cut(s) 370, 1188, 1254
BsmFI GGGAC 4 cut(s) 193, 293, 680, 1133
BsnI GGCC 5 cut(s) 198, 605, 1184, 1269, 1454
Bso31I GGTCTC 2 cut(s) 1188, 1254
BsoBI CYCGRG 1 cut(s) 589
Bsp1286I GDGCHC 4 cut(s) 328, 864, 945, 1162
Bsp143I GATC 5 cut(s) 331, 357, 639, 694, 797
Bsp19I CCATGG 3 cut(s) 193, 255, 353
BspACI CCGC 1 cut(s) 119
BspANI GGCC 5 cut(s) 198, 605, 1184, 1269, 1454
BspCNI CTCAG 3 cut(s) 418, 489, 774
BspLI GGNNCC 3 cut(s) 359, 1141, 1297
BspMAI CTGCAG 1 cut(s) 1090
BspPI GGATC 4 cut(s) 352, 365, 689, 805
BspT107I GGYRCC 1 cut(s) 1295
BspTNI GGTCTC 2 cut(s) 1188, 1254
BsrDI GCAATG 2 cut(s) 939, 1338
BsrFI RCCGGY 1 cut(s) 1298
BsrI ACTGG 2 cut(s) 362, 799
BssAI RCCGGY 1 cut(s) 1298
BssECI CCNNGG 6 cut(s) 130, 193, 255, 353, 606, 1444
BssMI GATC 5 cut(s) 331, 357, 639, 694, 797
BssNI GRCGYC 1 cut(s) 1296
BssT1I CCWWGG 4 cut(s) 193, 255, 353, 1444
Bst2UI CCWGG 3 cut(s) 40, 108, 607
Bst4CI ACNGT 5 cut(s) 307, 343, 671, 854, 1147
BstACI GRCGYC 1 cut(s) 1296
BstC8I GCNNGC 3 cut(s) 350, 1053, 1300
BstDEI CTNAG 5 cut(s) 405, 497, 683, 782, 1512
BstDSI CCRYGG 3 cut(s) 193, 255, 353
BstF5I GGATG 5 cut(s) 888, 914, 1216, 1429, 1455
BstH2I RGCGCY 1 cut(s) 1299
BstHHI GCGC 1 cut(s) 1298
BstKTI GATC 5 cut(s) 334, 360, 642, 697, 800
BstMAI GTCTC 3 cut(s) 370, 1188, 1254
BstMBI GATC 5 cut(s) 331, 357, 639, 694, 797
BstMWI GCNNNNNNNGC 2 cut(s) 182, 323
BstNI CCWGG 3 cut(s) 40, 108, 607
BstSCI CCNGG 4 cut(s) 38, 106, 605, 1315
BstSFI CTRYAG 1 cut(s) 1086
BstSLI GKGCMC 1 cut(s) 1162
BstV1I GCAGC 2 cut(s) 504, 539
BstX2I RGATCY 2 cut(s) 357, 797
BstXI CCANNNNNNTGG 3 cut(s) 344, 998, 1206
BstYI RGATCY 2 cut(s) 357, 797
BsuI GTATCC 1 cut(s) 817
BsuRI GGCC 5 cut(s) 198, 605, 1184, 1269, 1454
BtgI CCRYGG 3 cut(s) 193, 255, 353
BtsCI GGATG 5 cut(s) 888, 914, 1216, 1429, 1455
BtsIMutI CAGTG 2 cut(s) 339, 859
Cac8I GCNNGC 3 cut(s) 350, 1053, 1300
CaiI CAGNNNCTG 1 cut(s) 311
CfoI GCGC 1 cut(s) 1298
Cfr10I RCCGGY 1 cut(s) 1298
Cfr13I GGNCC 3 cut(s) 110, 197, 1182
CsiI ACCWGGT 1 cut(s) 106
Csp6I GTAC 1 cut(s) 1436
CviAII CATG 7 cut(s) 194, 256, 281, 354, 1179, 1414, 1420
CviQI GTAC 1 cut(s) 1436
DdeI CTNAG 5 cut(s) 405, 497, 683, 782, 1512
DinI GGCGCC 1 cut(s) 1297
DpnI GATC 5 cut(s) 333, 359, 641, 696, 799
DpnII GATC 5 cut(s) 331, 357, 639, 694, 797
EaeI YGGCCR 2 cut(s) 603, 1452
Ecl136II GAGCTC 2 cut(s) 862, 943
Eco130I CCWWGG 4 cut(s) 193, 255, 353, 1444
Eco24I GRGCYC 3 cut(s) 328, 864, 945
Eco31I GGTCTC 2 cut(s) 1188, 1254
Eco47I GGWCC 1 cut(s) 110
Eco53kI GAGCTC 2 cut(s) 862, 943
Eco57I CTGAAG 1 cut(s) 978
Eco88I CYCGRG 1 cut(s) 589
EcoICRI GAGCTC 2 cut(s) 862, 943
EcoRI GAATTC 1 cut(s) 1219
EcoRII CCWGG 3 cut(s) 38, 106, 605
EcoT14I CCWWGG 4 cut(s) 193, 255, 353, 1444
EcoT38I GRGCYC 3 cut(s) 328, 864, 945
EgeI GGCGCC 1 cut(s) 1297
EheI GGCGCC 1 cut(s) 1297
ErhI CCWWGG 4 cut(s) 193, 255, 353, 1444
FaeI CATG 7 cut(s) 197, 259, 284, 357, 1182, 1417, 1423
FaqI GGGAC 4 cut(s) 193, 293, 680, 1133
FatI CATG 7 cut(s) 193, 255, 280, 353, 1178, 1413, 1419
FauNDI CATATG 1 cut(s) 1205
FbaI TGATCA 1 cut(s) 331
Fnu4HI GCNGC 2 cut(s) 493, 528
FokI GGATG 5 cut(s) 875, 901, 1223, 1436, 1462
FriOI GRGCYC 3 cut(s) 328, 864, 945
Fsp4HI GCNGC 2 cut(s) 493, 528
FspBI CTAG 3 cut(s) 893, 1035, 1445
GlaI GCGC 1 cut(s) 1297
GluI GCNGC 2 cut(s) 493, 528
HaeII RGCGCY 1 cut(s) 1299
HaeIII GGCC 5 cut(s) 198, 605, 1184, 1269, 1454
HapII CCGG 4 cut(s) 1232, 1270, 1299, 1316
HhaI GCGC 1 cut(s) 1298
Hin1I GRCGYC 1 cut(s) 1296
Hin1II CATG 7 cut(s) 197, 259, 284, 357, 1182, 1417, 1423
Hin6I GCGC 1 cut(s) 1296
HinP1I GCGC 1 cut(s) 1296
HincII GTYRAC 2 cut(s) 514, 1174
HindII GTYRAC 2 cut(s) 514, 1174
HindIII AAGCTT 2 cut(s) 98, 1385
HinfI GANTC 2 cut(s) 1022, 1118
HpaI GTTAAC 1 cut(s) 1174
HpaII CCGG 4 cut(s) 1232, 1270, 1299, 1316
HphI GGTGA 4 cut(s) 28, 164, 326, 1432
Hpy166II GTNNAC 4 cut(s) 25, 514, 1160, 1174
Hpy188I TCNGA 4 cut(s) 134, 904, 1027, 1123
Hpy188III TCNNGA 2 cut(s) 75, 1150
Hpy8I GTNNAC 4 cut(s) 25, 514, 1160, 1174
Hpy99I CGWCG 1 cut(s) 453
HpyAV CCTTC 5 cut(s) 149, 702, 839, 1130, 1147
HpyCH4III ACNGT 5 cut(s) 307, 343, 671, 854, 1147
HpyCH4V TGCA 9 cut(s) 399, 628, 803, 949, 1051, 1081, 1088, 1160, 1345
HpyF10VI GCNNNNNNNGC 2 cut(s) 182, 323
HpyF3I CTNAG 5 cut(s) 405, 497, 683, 782, 1512
Hsp92I GRCGYC 1 cut(s) 1296
Hsp92II CATG 7 cut(s) 197, 259, 284, 357, 1182, 1417, 1423
HspAI GCGC 1 cut(s) 1296
KasI GGCGCC 1 cut(s) 1295
KroI GCCGGC 1 cut(s) 1298
KroNI GCCGGC 1 cut(s) 1300
Ksp22I TGATCA 1 cut(s) 331
KspAI GTTAAC 1 cut(s) 1174
Kzo9I GATC 5 cut(s) 331, 357, 639, 694, 797
LmnI GCTCC 5 cut(s) 95, 463, 778, 859, 1145
Lsp1109I GCAGC 2 cut(s) 504, 539
LweI GCATC 2 cut(s) 304, 421
MabI ACCWGGT 1 cut(s) 106
MaeI CTAG 3 cut(s) 893, 1035, 1445
MaeIII GTNAC 4 cut(s) 152, 226, 1098, 1399
MalI GATC 5 cut(s) 333, 359, 641, 696, 799
MboI GATC 5 cut(s) 331, 357, 639, 694, 797
MboII GAAGA 4 cut(s) 237, 466, 700, 1403
MflI RGATCY 2 cut(s) 357, 797
MhlI GDGCHC 4 cut(s) 328, 864, 945, 1162
MlsI TGGCCA 2 cut(s) 605, 1454
MluCI AATT 8 cut(s) 69, 476, 547, 564, 977, 996, 1219, 1273
MluNI TGGCCA 2 cut(s) 605, 1454
Mly113I GGCGCC 1 cut(s) 1296
MmeI TCCRAC 2 cut(s) 756, 801
Mox20I TGGCCA 2 cut(s) 605, 1454
MroNI GCCGGC 1 cut(s) 1298
MroXI GAANNNNTTC 3 cut(s) 1071, 1223, 1284
MscI TGGCCA 2 cut(s) 605, 1454
MseI TTAA 5 cut(s) 68, 987, 1062, 1173, 1349
MslI CAYNNNNRTG 2 cut(s) 925, 1418
Msp20I TGGCCA 2 cut(s) 605, 1454
MspI CCGG 4 cut(s) 1232, 1270, 1299, 1316
MspR9I CCNGG 4 cut(s) 40, 108, 607, 1317
MvaI CCWGG 3 cut(s) 40, 108, 607
MwoI GCNNNNNNNGC 2 cut(s) 182, 323
NaeI GCCGGC 1 cut(s) 1300
NarI GGCGCC 1 cut(s) 1296
NciI CCSGG 1 cut(s) 1317
NcoI CCATGG 3 cut(s) 193, 255, 353
NdeI CATATG 1 cut(s) 1205
NdeII GATC 5 cut(s) 331, 357, 639, 694, 797
NgoMIV GCCGGC 1 cut(s) 1298
NlaIII CATG 7 cut(s) 197, 259, 284, 357, 1182, 1417, 1423
NlaIV GGNNCC 3 cut(s) 359, 1141, 1297
NmuCI GTSAC 1 cut(s) 152
PdiI GCCGGC 1 cut(s) 1300
PdmI GAANNNNTTC 3 cut(s) 1071, 1223, 1284
PfeI GAWTC 2 cut(s) 1022, 1118
PfoI TCCNGGA 1 cut(s) 1315
PkrI GCNGC 2 cut(s) 494, 529
PluTI GGCGCC 1 cut(s) 1299
Psp124BI GAGCTC 2 cut(s) 864, 945
Psp6I CCWGG 3 cut(s) 38, 106, 605
PspGI CCWGG 3 cut(s) 38, 106, 605
PspN4I GGNNCC 3 cut(s) 359, 1141, 1297
PspPI GGNCC 3 cut(s) 110, 197, 1182
PstI CTGCAG 1 cut(s) 1090
PstNI CAGNNNCTG 1 cut(s) 311
PsuI RGATCY 2 cut(s) 357, 797
RsaI GTAC 1 cut(s) 1437
RsaNI GTAC 1 cut(s) 1436
RseI CAYNNNNRTG 2 cut(s) 925, 1418
SacI GAGCTC 2 cut(s) 864, 945
SaqAI TTAA 5 cut(s) 68, 987, 1062, 1173, 1349
SatI GCNGC 2 cut(s) 493, 528
Sau3AI GATC 5 cut(s) 331, 357, 639, 694, 797
Sau96I GGNCC 3 cut(s) 110, 197, 1182
ScrFI CCNGG 4 cut(s) 40, 108, 607, 1317
SduI GDGCHC 4 cut(s) 328, 864, 945, 1162
SexAI ACCWGGT 1 cut(s) 106
SfaNI GCATC 2 cut(s) 304, 421
SfcI CTRYAG 1 cut(s) 1086
SfoI GGCGCC 1 cut(s) 1297
SinI GGWCC 1 cut(s) 110
SmiMI CAYNNNNRTG 2 cut(s) 925, 1418
SmlI CTYRAG 1 cut(s) 1462
SmoI CTYRAG 1 cut(s) 1462
Sse9I AATT 8 cut(s) 69, 476, 547, 564, 977, 996, 1219, 1273
SsiI CCGC 1 cut(s) 119
SspDI GGCGCC 1 cut(s) 1295
SspI AATATT 1 cut(s) 634
SspMI CTAG 3 cut(s) 893, 1035, 1445
SstI GAGCTC 2 cut(s) 864, 945
StyD4I CCNGG 4 cut(s) 38, 106, 605, 1315
StyI CCWWGG 4 cut(s) 193, 255, 353, 1444
TaaI ACNGT 5 cut(s) 307, 343, 671, 854, 1147
TaqI TCGA 6 cut(s) 301, 322, 572, 642, 791, 1257
TasI AATT 8 cut(s) 69, 476, 547, 564, 977, 996, 1219, 1273
TatI WGTACW 1 cut(s) 1435
TfiI GAWTC 2 cut(s) 1022, 1118
Tru1I TTAA 5 cut(s) 68, 987, 1062, 1173, 1349
Tru9I TTAA 5 cut(s) 68, 987, 1062, 1173, 1349
TscAI CASTG 2 cut(s) 346, 859
TseFI GTSAC 1 cut(s) 152
TseI GCWGC 2 cut(s) 492, 527
Tsp45I GTSAC 1 cut(s) 152
TspDTI ATGAA 6 cut(s) 17, 237, 877, 903, 1212, 1430
TspGWI ACGGA 1 cut(s) 466
TspRI CASTG 2 cut(s) 346, 859
VneI GTGCAC 1 cut(s) 1158
VpaK11BI GGWCC 1 cut(s) 110
XapI RAATTY 2 cut(s) 1219, 1273
XmaJI CCTAGG 1 cut(s) 1444
XmnI GAANNNNTTC 3 cut(s) 1071, 1223, 1284
XspI CTAG 3 cut(s) 893, 1035, 1445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.