Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002164

Physical Location & Seq

Genomic Coordinates Forward (+)

19934987 .. 19941185

Gene Structure 6199 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G53430.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3117 bp

ATGGGTTTCATCTTCTCGACCGAGAAAGTTGTGTATGTTCTTCTTCTCATCTTCGTTTGCTTGGAAAATTTCGGATCAAATGCTCAACTTTTGCCTGAAGATGAAGTTCAAACATTGCGAACGATCTTTAGAAAGCTTCAAAACCAAACAGTGAACATCGAAAGAACTTCTTGTTCGGACCAAAACTGGAACTTTGTTGTCGAGTCAGCCTCCAACTCACCAACCAGTAACATTACCTGCGACTGTACTTTCAACGCCAGCTCAGTCTGTCGTGTCACAAACATACAGCTTAAAAGTTTCAGCTTGCCCGGAATATTCCCGCCTGAGTTCGGAAACCTCACGCGTCTTCGAGAGATAGATCTTTCGCGGAACTTTCTCAATGGAACAATACCTACGACATTGTCTCAAATTCCACTTGAAATCTTGTCTGTAATCGGAAACCGTCTCTCAGGACCATTTCCTCCTCAACTTGGAGATATTACTACACTTACTGATGTGAATTTGGAAACTAATTTATTCACAGGACCACTTCCTCGAAACCTAGGGAACTTAAGAAGCTTGAAAGAGTTACTTCTCTCTGCAAACAACTTCACGGGTCAAATCCCTGAGTCCTTAAGTAATCTCAAGAATTTGACTGAATTTCGGATTGATGGAAACTCTCTATCTGGGAAAATACCTGATTTTATTGGAAACTGGACTCTGCTGGAGAGGCTAGACCTTCAAGGCACATCAATGGAAGGTCCAATTCCACCTTCAATATCAAACTTGACGAACTTGACTGAATTGAGGATAACCGATTTGCGTGGACAAGCGGCCTTTTCTTTTCCGGACCTGCGAAATCTAATGAAAATGAAACGATTGGTACTAAGAAATTGTTTGATAAGGGGACCTATCCCGGAATACATTGGTTCCATGAGTGAGCTGAAGACACTAGATTTAAGCTCAAACATGCTAACCGGTGTAATTCCTGACACATTTCGGAATCTCGATGCATTTAACTTTATGTTTTTGAATAATAACTCATTGACTGGTCCAGTTCCTCAGTTCATCATCAACAGTAAAGAAAACCTAGATTTATCTGACAACAATTTCACTCAGCCACCTACTTTAAGCTGTAATCAGCTTGATGTGAACTTGATCTCCAGCTACCCTTCAGTAACCGATAACTCTGTTCAATGGTGCTTGAGAGAGGGTCTTCCATGTCCAGAAGATGCTAAACAGTCTTCCCTGTTCATCAACTGTGGAGGAAGCAGACTCAAGATTGGAAAAGACACTTATACAGATGACTTGAACAGTAGAGGACAATCAACATTCTCTTCTGTCTCTGAAAGATGGGGATACAGTAGTTCTGGAGTTTGGTTAGGCAAGGAGGATGCTGGCTACTTAGCAACAGACAGATTTAACTTGATCAATGGATCAACTCCAGAGTATTACAAAACAGCCCGTCTCTCTCCACAGTCACTCAAGTACTATGGACTATGCTTGAGAAGAGGAAGTTACAAACTGCAGCTACATTTTGCAGAGATAATGTTCTCAAATGACCAGACTTTTAATAGCTTAGGGCGGCGAATATTCGACATTTATGTCCAAGGGAACTTGTTGGAGAGGGACTTTAACATAGCAGAGAGAGCAGGTGGAGTTGGTAAACCGTTCATAAGGCAAATTGATGGAGTTCAAGTGAATGGAAGTACGTTAGAGATTCATTTGCAGTGGACAGGGAAAGGCACAAACGTAATACCAACAAGAGGTGTTTACGGGCCTCTCATATCTGCCATAACCATTACACCAAATTTCAAGGTTGATACCGGAAAACCATTGTCCAATGGAGCAGTTGCAGGCATTGTAATCGCAGCGTGTGCGGTTTTCGGGTTGCTGGTACTTGTAATCTTGAGGCTTACAGGTTACTTAGGTGGAAAAGAAGTAGATGAGAATGAAGAGCTTCGGGGACTTGATTTGCAGACAGGATCGTTCACATTGAAACAAATAAAACGTGCTACTAATAACTTTGATCCAGAAAACAAGATTGGTGAAGGAGGATTTGGACCGGTTTATAAGGGTGTTCTCGCTGATGGGATGACCATAGCGGTGAAGCAGCTTTCATCAAAATCTAAGCAAGGAAACCGAGAATTTGTGACTGAGATCGGTATGATATCTGCGCTGCAACACCCTAATCTTGTGAAACTTTATGGTTGTTGCATCGAAGGGAAAGAGCTCTTGCTTGTGTATGAGTACTTAGAGAACAACAGTCTCGCTCGCGCACTCTTTGGCACAGAGAAACAGAGGCTTCACTTGGATTGGTCAACAAGGAACAAGATTTGCATAGGGATTGCGAAAGGATTGGCCTATCTACACGAGGAATCAAGGCTGAAGATTGTTCATAGAGACATAAAAGCGACAAATGTGCTTCTTGATCTGTCTCTAAACGCTAAGATCTCTGATTTTGGTCTAGCTAAACTCAACGATGACGAGAATACACATATCAGCACAAGGATTGCAGGAACAATAGGTTACATGGCTCCTGAGTATGCAATGAGAGGTTACTTGACAGACAAGGCAGATGTTTACAGCTTTGGTGTTGTCTGTTTAGAGATTGTTAGTGGAAAGAGCAACACAAATTACAGACCAAAGGAAGAGTTTGTTTACCTTCTGGATTGGGCATATGTCTTGCAAGAACAAGGTAGTCTTCTAGAACTCGTTGATCCGGATCTCGGCACAAGCTTTTCGAAGAAAGAAGCGATGAGGATGTTGAACATAGCATTACTCTGCACGAACCCATCTCCAACATTGAGACCACCAATGTCATCTGTTGTAAGTATGTTAGAAGGAAAAATCAAAGTCCAACCACCATTGGTGAAACGTGAAGCAGATCCAAGTGGTTCAGCAGCAATGAGGTTTAAGGCCTTAGAGCTTTTGTCACAAGACAGTGAGTCACAAGTCTCGACATATGCAAGAAACAGAGAGCAAGACATAAGCTCCTCATCGATGGACGGTCCTTGGGTAGATTCTTCCTTCTCTGAGCCTGGGAAAGATGTTAGCCTGCAACAGCAAGAAGAAGGACGTTCATCATCTTCGTCGAGGAAACTTTTAGATGATCTTACCGATGTGAAGATTGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_1 LRR_8 Malectin Pkinase Pkinase_Tyr

Protein Analysis

1038

Amino Acids

114.94

Weight (kDa)

5.63

Isoelectric Point (pI)

37.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	142 - 231	8.5e-09	Leucine-rich repeat region
Malectin	PF11721	368 - 551	1.5e-44	Malectin domain
Pkinase	PF00069	630 - 831	1.2e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	631 - 898	2.6e-49	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002164)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53430 AT1G53430 AT1G53440
fragaria_vesca	FvH4_3g17940
malus_domestica	MD03G1247100.v1.1 MD03G1248200.v1.1 MD03G1248300.v1.1 MD03G1249100.v1.1 MD03G1249200.v1.1 MD11G1267500.v1.1
prunus_persica	Prupe.4G158000_v2.0.a1 Prupe.4G158000_v2.0.a1
pyrus_communis	pycom03g19620 pycom03g19750 pycom06g03840 pycom11g23530
rosa_chinensis	RchiOBHm_Chr5g0029911 RchiOBHm_Chr5g0029981
rosa_laevigata	RLG00000033201 RLG00000033206
rosa_multiflora	Rmu_sc0003368.1_g000014 Rmu_sc0004542.1_g000020 Rmu_sc0021047.1_g000001
rosa_roxburghii	Rroxscaffold_1G00050050
rosa_rugosa	Rorug05G0115300 Rorug05G0115400 Rorug05G0115500 Rorug05G0115600 Rorug05G0115700 Rorug05G0115800 Rorug05G0115900 Rorug05G0116000 Rorug05G0116100 Rorug05G0116200
rosa_samantha	Rh5AG208900 Rh5AG209100 Rh5BG207700 Rh5CG231100 Rh5CG231800 Rh5DG211900 Rh5DG212500
rosa_wichuraiana	Rw5G019010 Rw5G019050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2052
AarI	CACCTGC	1 cut(s)	1622
AasI	GACNNNNNNGTC	1 cut(s)	1583
Acc36I	ACCTGC	3 cut(s)	245, 840, 1622
AccII	CGCG	3 cut(s)	343, 367, 2256
AccIII	TCCGGA	2 cut(s)	826, 2701
AciI	CCGC	6 cut(s)	320, 367, 812, 1564, 1859, 2084
AclWI	GGATC	7 cut(s)	82, 1423, 1972, 2003, 2693, 2712, 2861
AcsI	RAATTY	7 cut(s)	67, 408, 499, 628, 638, 1789, 2126
AcuI	CTGAAG	4 cut(s)	117, 944, 1137, 2387
AfaI	GTAC	6 cut(s)	247, 864, 1469, 1690, 1878, 2231
AfiI	CCNNNNNNNGG	4 cut(s)	329, 470, 1745, 2708
AflII	CTTAAG	2 cut(s)	550, 613
AflIII	ACRYGT	1 cut(s)	341
AgeI	ACCGGT	2 cut(s)	956, 2044
AhdI	GACNNNNNGTC	1 cut(s)	2926
AjnI	CCWGG	1 cut(s)	3019
AjuI	GAANNNNNNNTTGG	6 cut(s)	736, 768, 2007, 2022, 2039, 2054
AloI	GAACNNNNNNTCC	2 cut(s)	1124, 1156
Alw21I	GWGCWC	1 cut(s)	2214
Alw26I	GTCTC	9 cut(s)	408, 449, 1327, 1451, 2252, 2376, 2421, 2782, 2941
AlwI	GGATC	7 cut(s)	82, 1423, 1972, 2003, 2693, 2712, 2861
Aor13HI	TCCGGA	2 cut(s)	826, 2701
AoxI	GGCC	4 cut(s)	813, 1757, 2340, 2898
ApeKI	GCWGC	5 cut(s)	1507, 1850, 2092, 2158, 2882
ApoI	RAATTY	7 cut(s)	67, 408, 499, 628, 638, 1789, 2126
AsiGI	ACCGGT	2 cut(s)	956, 2044
Asp700I	GAANNNNTTC	1 cut(s)	1938
AspA2I	CCTAGG	1 cut(s)	541
AspLEI	GCGC	2 cut(s)	2158, 2258
AsuC2I	CCSGG	2 cut(s)	309, 896
AsuHPI	GGTGA	4 cut(s)	210, 2039, 2098, 2863
AsuII	TTCGAA	1 cut(s)	2723
AvaII	GGWCC	9 cut(s)	178, 452, 524, 740, 829, 887, 1031, 2042, 2990
AvrII	CCTAGG	1 cut(s)	541
BaeI	ACNNNNGTAYC	2 cut(s)	1330, 1363
BanII	GRGCYC	1 cut(s)	2214
BauI	CACGAG	1 cut(s)	2351
BbsI	GAAGAC	5 cut(s)	338, 932, 1187, 1215, 2675
Bbv12I	GWGCWC	1 cut(s)	2214
BbvI	GCAGC	5 cut(s)	1519, 1862, 2104, 2145, 2894
BccI	CCATC	6 cut(s)	644, 1326, 1661, 2063, 2782, 2977
BcgI	CGANNNNNNTGC	4 cut(s)	1828, 1862, 2383, 2417
BciT130I	CCWGG	1 cut(s)	3021
BciVI	GTATCC	1 cut(s)	1331
BclI	TGATCA	1 cut(s)	1407
BcnI	CCSGG	2 cut(s)	309, 896
BcoDI	GTCTC	9 cut(s)	408, 449, 1327, 1451, 2252, 2376, 2421, 2782, 2941
BfaI	CTAG	6 cut(s)	542, 713, 932, 1070, 2447, 2687
BfmI	CTRYAG	1 cut(s)	1505
BfrI	CTTAAG	2 cut(s)	550, 613
BfuAI	ACCTGC	3 cut(s)	245, 840, 1622
BfuI	GTATCC	1 cut(s)	1331
BglII	AGATCT	2 cut(s)	358, 2430
BisI	GCNGC	7 cut(s)	813, 1508, 1565, 1851, 2093, 2159, 2883
BlnI	CCTAGG	1 cut(s)	541
BlsI	GCNGC	7 cut(s)	814, 1509, 1566, 1852, 2094, 2160, 2884
BmcAI	AGTACT	2 cut(s)	1469, 2231
Bme1390I	CCNGG	3 cut(s)	309, 896, 3021
Bme18I	GGWCC	9 cut(s)	178, 452, 524, 740, 829, 887, 1031, 2042, 2990
BmeRI	GACNNNNNGTC	1 cut(s)	2926
BmiI	GGNNCC	3 cut(s)	888, 910, 2517
BmrFI	CCNGG	3 cut(s)	309, 896, 3021
BmsI	GCATC	4 cut(s)	979, 1201, 1363, 2205
BpiI	GAAGAC	5 cut(s)	338, 932, 1187, 1215, 2675
BplI	GAGNNNNNCTC	2 cut(s)	194, 226
BpmI	CTGGAG	4 cut(s)	725, 1126, 1371, 1407
Bpu10I	CCTNAGC	1 cut(s)	1558
Bpu14I	TTCGAA	1 cut(s)	2723
BpuEI	CTTGAG	6 cut(s)	608, 1204, 1241, 1448, 1504, 1909
BpuMI	CCSGG	2 cut(s)	309, 896
Bsa29I	ATCGAT	1 cut(s)	2981
BsaBI	GATNNNNATC	1 cut(s)	2703
BsaI	GGTCTC	1 cut(s)	2782
BsaJI	CCNNGG	4 cut(s)	541, 1588, 2993, 3020
BsaWI	WCCGGW	5 cut(s)	826, 956, 1805, 2044, 2701
BsaXI	ACNNNNNCTCC	6 cut(s)	1124, 1154, 1662, 1692, 2957, 2987
Bsc4I	CCNNNNNNNGG	4 cut(s)	329, 470, 1745, 2708
Bse118I	RCCGGY	2 cut(s)	956, 2044
Bse1I	ACTGG	5 cut(s)	191, 225, 698, 1033, 1034
Bse3DI	GCAATG	3 cut(s)	113, 2535, 2892
Bse8I	GATNNNNATC	1 cut(s)	2703
BseAI	TCCGGA	2 cut(s)	826, 2701
BseBI	CCWGG	1 cut(s)	3021
BseCI	ATCGAT	1 cut(s)	2981
BseDI	CCNNGG	4 cut(s)	541, 1588, 2993, 3020
BseGI	GGATG	3 cut(s)	1378, 2079, 2748
BseJI	GATNNNNATC	1 cut(s)	2703
BseLI	CCNNNNNNNGG	4 cut(s)	329, 470, 1745, 2708
BseMI	GCAATG	3 cut(s)	113, 2535, 2892
BseMII	CTCAG	9 cut(s)	276, 315, 462, 597, 1055, 1109, 2127, 2511, 3006
BseNI	ACTGG	5 cut(s)	191, 225, 698, 1033, 1034
BseRI	GAGGAG	2 cut(s)	453, 2965
BseXI	GCAGC	5 cut(s)	1519, 1862, 2104, 2145, 2894
BsgI	GTGCAG	1 cut(s)	2749
Bsh1236I	CGCG	3 cut(s)	343, 367, 2256
Bsh1285I	CGRYCG	1 cut(s)	21
BshFI	GGCC	4 cut(s)	815, 1759, 2342, 2900
BshTI	ACCGGT	2 cut(s)	956, 2044
BshVI	ATCGAT	1 cut(s)	2981
BsiEI	CGRYCG	1 cut(s)	21
BsiHKAI	GWGCWC	1 cut(s)	2214
BsiSI	CCGG	7 cut(s)	309, 827, 896, 957, 1806, 2045, 2702
BslFI	GGGAC	3 cut(s)	900, 1622, 1959
BslI	CCNNNNNNNGG	4 cut(s)	329, 470, 1745, 2708
BsmAI	GTCTC	9 cut(s)	408, 449, 1327, 1451, 2252, 2376, 2421, 2782, 2941
BsmBI	CGTCTC	2 cut(s)	449, 1451
BsmFI	GGGAC	3 cut(s)	900, 1622, 1959
BsnI	GGCC	4 cut(s)	815, 1759, 2342, 2900
Bso31I	GGTCTC	1 cut(s)	2782
Bsp119I	TTCGAA	1 cut(s)	2723
Bsp1286I	GDGCHC	1 cut(s)	2214
Bsp13I	TCCGGA	2 cut(s)	826, 2701
BspACI	CCGC	6 cut(s)	320, 367, 812, 1564, 1859, 2084
BspANI	GGCC	4 cut(s)	815, 1759, 2342, 2900
BspCNI	CTCAG	9 cut(s)	275, 316, 461, 598, 1054, 1108, 2128, 2512, 3007
BspDI	ATCGAT	1 cut(s)	2981
BspEI	TCCGGA	2 cut(s)	826, 2701
BspFNI	CGCG	3 cut(s)	343, 367, 2256
BspLI	GGNNCC	3 cut(s)	888, 910, 2517
BspMAI	CTGCAG	1 cut(s)	1509
BspMI	ACCTGC	3 cut(s)	245, 840, 1622
BspPI	GGATC	7 cut(s)	82, 1423, 1972, 2003, 2693, 2712, 2861
BspQI	GCTCTTC	1 cut(s)	1929
BspT104I	TTCGAA	1 cut(s)	2723
BspTI	CTTAAG	2 cut(s)	550, 613
BspTNI	GGTCTC	1 cut(s)	2782
BsrDI	GCAATG	3 cut(s)	113, 2535, 2892
BsrFI	RCCGGY	2 cut(s)	956, 2044
BsrI	ACTGG	5 cut(s)	191, 225, 698, 1033, 1034
BssAI	RCCGGY	2 cut(s)	956, 2044
BssECI	CCNNGG	4 cut(s)	541, 1588, 2993, 3020
BssSI	CACGAG	1 cut(s)	2351
BssT1I	CCWWGG	3 cut(s)	541, 1588, 2993
Bst2BI	CACGAG	1 cut(s)	2351
Bst2UI	CCWGG	1 cut(s)	3021
Bst6I	CTCTTC	4 cut(s)	1321, 1483, 1929, 2625
BstAFI	CTTAAG	2 cut(s)	550, 613
BstAPI	GCANNNNNTGC	1 cut(s)	1856
BstBI	TTCGAA	1 cut(s)	2723
BstC8I	GCNNGC	6 cut(s)	259, 305, 1378, 1837, 2254, 3038
BstF5I	GGATG	3 cut(s)	1378, 2079, 2748
BstFNI	CGCG	3 cut(s)	343, 367, 2256
BstHHI	GCGC	2 cut(s)	2158, 2258
BstMAI	GTCTC	9 cut(s)	408, 449, 1327, 1451, 2252, 2376, 2421, 2782, 2941
BstMCI	CGRYCG	1 cut(s)	21
BstMWI	GCNNNNNNNGC	3 cut(s)	709, 1628, 1856
BstNI	CCWGG	1 cut(s)	3021
BstNSI	RCATGY	1 cut(s)	952
BstSCI	CCNGG	3 cut(s)	307, 894, 3019
BstSFI	CTRYAG	1 cut(s)	1505
BstUI	CGCG	3 cut(s)	343, 367, 2256
BstV1I	GCAGC	5 cut(s)	1519, 1862, 2104, 2145, 2894
BstV2I	GAAGAC	5 cut(s)	338, 932, 1187, 1215, 2675
BstX2I	RGATCY	4 cut(s)	358, 2430, 2704, 2866
BstYI	RGATCY	4 cut(s)	358, 2430, 2704, 2866
Bsu15I	ATCGAT	1 cut(s)	2981
BsuI	GTATCC	1 cut(s)	1331
BsuRI	GGCC	4 cut(s)	815, 1759, 2342, 2900
BsuTUI	ATCGAT	1 cut(s)	2981
BtgZI	GCGATG	1 cut(s)	2750
BtsCI	GGATG	3 cut(s)	1378, 2079, 2748
BtsI	GCAGTG	1 cut(s)	1715
BtsIMutI	CAGTG	3 cut(s)	156, 1715, 2929
BveI	ACCTGC	3 cut(s)	245, 840, 1622
Cac8I	GCNNGC	6 cut(s)	259, 305, 1378, 1837, 2254, 3038
CfoI	GCGC	2 cut(s)	2158, 2258
Cfr10I	RCCGGY	2 cut(s)	956, 2044
ClaI	ATCGAT	1 cut(s)	2981
CseI	GACGC	1 cut(s)	332
Csp6I	GTAC	6 cut(s)	246, 863, 1468, 1689, 1877, 2230
CspAI	ACCGGT	2 cut(s)	956, 2044
CviAII	CATG	4 cut(s)	913, 949, 1200, 2512
CviQI	GTAC	6 cut(s)	246, 863, 1468, 1689, 1877, 2230
DrdI	GACNNNNNNGTC	1 cut(s)	1583
DriI	GACNNNNNGTC	1 cut(s)	2926
DseDI	GACNNNNNNGTC	1 cut(s)	1583
Eam1104I	CTCTTC	4 cut(s)	1321, 1483, 1929, 2625
Eam1105I	GACNNNNNGTC	1 cut(s)	2926
EarI	CTCTTC	4 cut(s)	1321, 1483, 1929, 2625
Ecl136II	GAGCTC	1 cut(s)	2212
Eco130I	CCWWGG	3 cut(s)	541, 1588, 2993
Eco147I	AGGCCT	1 cut(s)	2900
Eco24I	GRGCYC	1 cut(s)	2214
Eco31I	GGTCTC	1 cut(s)	2782
Eco32I	GATATC	1 cut(s)	2151
Eco47I	GGWCC	9 cut(s)	178, 452, 524, 740, 829, 887, 1031, 2042, 2990
Eco53kI	GAGCTC	1 cut(s)	2212
Eco57I	CTGAAG	4 cut(s)	117, 944, 1137, 2387
EcoICRI	GAGCTC	1 cut(s)	2212
EcoO109I	RGGNCCY	1 cut(s)	887
EcoRII	CCWGG	1 cut(s)	3019
EcoRV	GATATC	1 cut(s)	2151
EcoT14I	CCWWGG	3 cut(s)	541, 1588, 2993
EcoT22I	ATGCAT	1 cut(s)	994
EcoT38I	GRGCYC	1 cut(s)	2214
ErhI	CCWWGG	3 cut(s)	541, 1588, 2993
Esp3I	CGTCTC	2 cut(s)	449, 1451
FaeI	CATG	4 cut(s)	916, 952, 1203, 2515
FalI	AAGNNNNNCTT	6 cut(s)	154, 186, 555, 587, 2667, 2699
FaqI	GGGAC	3 cut(s)	900, 1622, 1959
FatI	CATG	4 cut(s)	912, 948, 1199, 2511
FauI	CCCGC	1 cut(s)	327
FauNDI	CATATG	2 cut(s)	2659, 2944
FbaI	TGATCA	1 cut(s)	1407
Fnu4HI	GCNGC	7 cut(s)	813, 1508, 1565, 1851, 2093, 2159, 2883
FokI	GGATG	3 cut(s)	1385, 2086, 2755
FriOI	GRGCYC	1 cut(s)	2214
Fsp4HI	GCNGC	7 cut(s)	813, 1508, 1565, 1851, 2093, 2159, 2883
FspBI	CTAG	6 cut(s)	542, 713, 932, 1070, 2447, 2687
GlaI	GCGC	2 cut(s)	2157, 2257
GluI	GCNGC	7 cut(s)	813, 1508, 1565, 1851, 2093, 2159, 2883
GsuI	CTGGAG	4 cut(s)	725, 1126, 1371, 1407
HaeIII	GGCC	4 cut(s)	815, 1759, 2342, 2900
HapII	CCGG	7 cut(s)	309, 827, 896, 957, 1806, 2045, 2702
HgaI	GACGC	1 cut(s)	332
HhaI	GCGC	2 cut(s)	2158, 2258
Hin1II	CATG	4 cut(s)	916, 952, 1203, 2515
Hin6I	GCGC	2 cut(s)	2156, 2256
HinP1I	GCGC	2 cut(s)	2156, 2256
HincII	GTYRAC	1 cut(s)	2301
HindII	GTYRAC	1 cut(s)	2301
HindIII	AAGCTT	3 cut(s)	134, 556, 2716
HinfI	GANTC	9 cut(s)	203, 608, 697, 982, 1254, 1699, 2357, 2927, 3002
HpaII	CCGG	7 cut(s)	309, 827, 896, 957, 1806, 2045, 2702
HphI	GGTGA	4 cut(s)	210, 2039, 2098, 2863
Hpy99I	CGWCG	1 cut(s)	3076
HpyCH4IV	ACGT	5 cut(s)	1691, 1731, 1990, 2857, 3058
HpyF10VI	GCNNNNNNNGC	3 cut(s)	709, 1628, 1856
HpySE526I	ACGT	5 cut(s)	1691, 1731, 1990, 2857, 3058
Hsp92II	CATG	4 cut(s)	916, 952, 1203, 2515
HspAI	GCGC	2 cut(s)	2156, 2256
Kpn2I	TCCGGA	2 cut(s)	826, 2701
Ksp22I	TGATCA	1 cut(s)	1407
LguI	GCTCTTC	1 cut(s)	1929
LmnI	GCTCC	3 cut(s)	1826, 2521, 2978
Lsp1109I	GCAGC	5 cut(s)	1519, 1862, 2104, 2145, 2894
LweI	GCATC	4 cut(s)	979, 1201, 1363, 2205
MaeI	CTAG	6 cut(s)	542, 713, 932, 1070, 2447, 2687
MaeII	ACGT	5 cut(s)	1691, 1731, 1990, 2857, 3058
MflI	RGATCY	4 cut(s)	358, 2430, 2704, 2866
MhlI	GDGCHC	1 cut(s)	2214
MluI	ACGCGT	1 cut(s)	341
MlyI	GAGTC	5 cut(s)	212, 617, 691, 1248, 2936
MmeI	TCCRAC	4 cut(s)	237, 1581, 2804, 2863
Mph1103I	ATGCAT	1 cut(s)	994
MroI	TCCGGA	2 cut(s)	826, 2701
MroXI	GAANNNNTTC	1 cut(s)	1938
MslI	CAYNNNNRTG	2 cut(s)	731, 2084
MspCI	CTTAAG	2 cut(s)	550, 613
MspI	CCGG	7 cut(s)	309, 827, 896, 957, 1806, 2045, 2702
MspR9I	CCNGG	3 cut(s)	309, 896, 3021
MvaI	CCWGG	1 cut(s)	3021
MvnI	CGCG	3 cut(s)	343, 367, 2256
MwoI	GCNNNNNNNGC	3 cut(s)	709, 1628, 1856
NciI	CCSGG	2 cut(s)	309, 896
NdeI	CATATG	2 cut(s)	2659, 2944
NlaIII	CATG	4 cut(s)	916, 952, 1203, 2515
NlaIV	GGNNCC	3 cut(s)	888, 910, 2517
NmeAIII	GCCGAG	1 cut(s)	2688
NmuCI	GTSAC	5 cut(s)	274, 1458, 2131, 2913, 2928
NsiI	ATGCAT	1 cut(s)	994
NspI	RCATGY	1 cut(s)	952
NspV	TTCGAA	1 cut(s)	2723
PaqCI	CACCTGC	1 cut(s)	1622
PceI	AGGCCT	1 cut(s)	2900
PciSI	GCTCTTC	1 cut(s)	1929
PdmI	GAANNNNTTC	1 cut(s)	1938
PfeI	GAWTC	4 cut(s)	982, 1699, 2357, 3002
PflFI	GACNNNGTC	1 cut(s)	400
PfoI	TCCNGGA	1 cut(s)	894
PinAI	ACCGGT	2 cut(s)	956, 2044
PkrI	GCNGC	7 cut(s)	814, 1509, 1566, 1852, 2094, 2160, 2884
PleI	GAGTC	5 cut(s)	211, 616, 691, 1248, 2935
PpsI	GAGTC	5 cut(s)	211, 616, 691, 1248, 2935
PpuMI	RGGWCCY	1 cut(s)	887
PsiI	TTATAA	1 cut(s)	2052
Psp124BI	GAGCTC	1 cut(s)	2214
Psp5II	RGGWCCY	1 cut(s)	887
Psp6I	CCWGG	1 cut(s)	3019
PspGI	CCWGG	1 cut(s)	3019
PspN4I	GGNNCC	3 cut(s)	888, 910, 2517
PspPPI	RGGWCCY	1 cut(s)	887
PsrI	GAACNNNNNNTAC	2 cut(s)	376, 408
PstI	CTGCAG	1 cut(s)	1509
PsuI	RGATCY	4 cut(s)	358, 2430, 2704, 2866
PsyI	GACNNNGTC	1 cut(s)	400
RsaI	GTAC	6 cut(s)	247, 864, 1469, 1690, 1878, 2231
RsaNI	GTAC	6 cut(s)	246, 863, 1468, 1689, 1877, 2230
RseI	CAYNNNNRTG	2 cut(s)	731, 2084
SacI	GAGCTC	1 cut(s)	2214
SapI	GCTCTTC	1 cut(s)	1929
SatI	GCNGC	7 cut(s)	813, 1508, 1565, 1851, 2093, 2159, 2883
ScaI	AGTACT	2 cut(s)	1469, 2231
SchI	GAGTC	5 cut(s)	212, 617, 691, 1248, 2936
ScrFI	CCNGG	3 cut(s)	309, 896, 3021
SduI	GDGCHC	1 cut(s)	2214
SfaNI	GCATC	4 cut(s)	979, 1201, 1363, 2205
SfcI	CTRYAG	1 cut(s)	1505
SfuI	TTCGAA	1 cut(s)	2723
SinI	GGWCC	9 cut(s)	178, 452, 524, 740, 829, 887, 1031, 2042, 2990
SmiMI	CAYNNNNRTG	2 cut(s)	731, 2084
SmlI	CTYRAG	8 cut(s)	550, 613, 623, 1183, 1256, 1463, 1483, 1888
SmoI	CTYRAG	8 cut(s)	550, 613, 623, 1183, 1256, 1463, 1483, 1888
SseBI	AGGCCT	1 cut(s)	2900
SsiI	CCGC	6 cut(s)	320, 367, 812, 1564, 1859, 2084
SspI	AATATT	2 cut(s)	315, 1572
SspMI	CTAG	6 cut(s)	542, 713, 932, 1070, 2447, 2687
SstI	GAGCTC	1 cut(s)	2214
StuI	AGGCCT	1 cut(s)	2900
StyD4I	CCNGG	3 cut(s)	307, 894, 3019
StyI	CCWWGG	3 cut(s)	541, 1588, 2993
TaiI	ACGT	5 cut(s)	1694, 1734, 1993, 2860, 3061
TaqII	GACCGA	1 cut(s)	35
TatI	WGTACW	3 cut(s)	245, 1467, 2229
TauI	GCSGC	2 cut(s)	815, 1567
TfiI	GAWTC	4 cut(s)	982, 1699, 2357, 3002
TscAI	CASTG	3 cut(s)	156, 1715, 2929
TseFI	GTSAC	5 cut(s)	274, 1458, 2131, 2913, 2928
TseI	GCWGC	5 cut(s)	1507, 1850, 2092, 2158, 2882
Tsp45I	GTSAC	5 cut(s)	274, 1458, 2131, 2913, 2928
TspRI	CASTG	3 cut(s)	156, 1715, 2929
Tth111I	GACNNNGTC	1 cut(s)	400
Vha464I	CTTAAG	2 cut(s)	550, 613
VpaK11BI	GGWCC	9 cut(s)	178, 452, 524, 740, 829, 887, 1031, 2042, 2990
XapI	RAATTY	7 cut(s)	67, 408, 499, 628, 638, 1789, 2126
XbaI	TCTAGA	1 cut(s)	2686
XceI	RCATGY	1 cut(s)	952
XmaJI	CCTAGG	1 cut(s)	541
XmnI	GAANNNNTTC	1 cut(s)	1938
XspI	CTAG	6 cut(s)	542, 713, 932, 1070, 2447, 2687
ZrmI	AGTACT	2 cut(s)	1469, 2231
Zsp2I	ATGCAT	1 cut(s)	994

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.