Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0002164

Physical Location & Seq

Genomic Coordinates Forward (+)

33546097 .. 33554057

Gene Structure 7961 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1248300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2244 bp

ATGCCTTTTCCTACTCTGAGATATATGAAGGACATACAGATGATGATTTTGAGAAATTGCTCAATTAGTGGTACAATCCCTAATTACCTTGGCGAGCTGACAAAACTAGTAACATTAGACTTAAGCTTTAACAAGTTGACTGGAGAAATTCCACAGACAATTGAGCGTGCTAGCCATTTATCTTTCATGTCTTTGAGCAACAACATACTGACCGGCGAAGTACCAATTTGGATATTGCAGAGCAAACGTTACCGGGACTTGTCATACAACAATTTTACGGGATCACCTTCCGTCAGCTGTCAACACTTGACTGTGAATTTGGTTTCTAGTTATTCGTCGCCAGAAAACTTGTGGTGCTTGAAGAAGGACCTTCCTTGCTCCTCAAAACCAAAATACCATTCTATATTTATCAATTGTGGAGGGAGGAAGACTAAGTTTGATGGCCATACATATGAAGAGGACTTGAGCCCTTTCGGACCATCACACTTTGTTTCGTCGGCTCAAGAGTGGGGCTACAGCAGTACCGGTGTGGACAAGAGCAACCCTGATGCAAATTACATCGTAACATCTTTGAACATTACCGGGCCAGATTTATATCAAACAGCGCGCATTGCCCCTCTTTCACTTAAGTACTATGGCCTTTGCATGCTCAAAGGCAGCTACAAAGTGCGGTTGTACTTTTCCGAGATCATGTTCACGGATGACCAAACTTTTAGCAGTCTTGGAAGACGCTTGTTTGACATCTCAATTCAAGGGAACTTGGTTTTGAAAGACTTCAACATTATGGAAGAAGCAAGAGGTGTCGGTAAGGGCATTGTCAGAGAGTTTGACAATGTCACCGTTATTGGTAGCACACTGGAGATCCACCTATATTGGGCAGGAAAAGGGACAACTGCCATTCCTTATACAGGTGTCTACGGGCCTCTTATATCGGCCATTAGTGTGACGCCAAACTTTGAACCTCCAAAAAAAACTTTAAGCTACTTAGAGCGTATTAGGAGTCATGTGCCTATAGGCACAATGGTAGGAATTGGATGCGCAATAGCAGTCGTCATCATCGCCATCGTATTACTAGCGCTCGTTATTCTTTGGAAGAGAGGTCTCCTAGGACGACAAAAAACATTGGAGGATGATTTGAAAGGTGTGGAACTACAAACTCGCAAGTTTACCTTTACACAACTCAAAGATGCCACAACCAACTTTGACCAAGCCAATAAGATTGGTGAAGGTGGTTTTGGTACTGTTTATAAGGGCATTCTTGTAGATGGAACCCTGATTGCTGTTAAGCAGCTTTCTTCCAAGTCAAAGCAAGGAAATCGGGAATTTGTGAATGAGATAGGCATGATTTCTGCTTTGCAACATCCTCATCTTGTAAACCTATACGGATGTTGTATTGAAGGAAATCAACTCTACCTTGTCTACGAGTACATGGAAAACAATAGCGTTTCTCATGCTTTGTTTGGGGCAGAAGAAAGTCAGTTGAAGTTGGATTGGCCAACGAGGCATAGGATATGTGTTGGCATAGCAAAAGGTTTGGCTTATCTTCACGAGGAATCAAGCTTGAAGGTTATCCATAGAGATATCAAGGCCACGAACGTGTTGCTTGATAGACATCTTAACCCGAAGATCTCGGACTTTGGGTTGGCCAAACTTGCTGACGAAGATAAAACACATATAAGCACTCGTGCTGCTGGAACTTTTGGATACATGGCACCAGAATATGCACTTCGGGGTCATCTCACAGACAAAGCAGATGTCTACAGCTTTGGAATTGTTGTACTGGAGATTATCAGTGGGAAGAAGAACTCAACTCAGCAGACAAATGCAGAAGGATTTTATATTCTTGATTGGGCGCATGTTCTCAAATCGGAAGGGAATTTGATGAACTTAGTGGATCCAAAGTTGAGCGCCGAATTCAACAATGAAGAGATGTTGGCTACAATCAATGTAGCTCTCCACTGCTGTAATTCCACTTCGAAGCTTAGGCCCACTATGTCTGCAGTTGTGAATATGCTCGAAGGTAAGGCTGCTGTTGAAGAGTTTGTCTTGGACCCCAATCCCTCGACAAGTGATAACATGGACCCAACGTTGAAGAAACTTTTCCAGTTTTGTTTGAGAGGGGGTGCCGGGTCAGTTGAAGGGCCATCGAATGATTCATTTGCAGCTCTTGATGATATCCATCCATACAATCCTCGTTCAGGTCGCCAAGACGATAGAAAAGGGAGAAAATGGCACAGTTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_1 LRR_8 Malectin Pkinase Pkinase_Tyr

Protein Analysis

748

Amino Acids

83.04

Weight (kDa)

7.57

Isoelectric Point (pI)

32.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Malectin	PF11721	135 - 313	9.2e-37	Malectin domain
Pkinase	PF00069	402 - 670	5.8e-49	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	404 - 672	3.6e-50	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002164)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53430 AT1G53430 AT1G53440
fragaria_vesca	FvH4_3g17940
malus_domestica	MD03G1247100.v1.1 MD03G1248200.v1.1 MD03G1248300.v1.1 MD03G1249100.v1.1 MD03G1249200.v1.1 MD11G1267500.v1.1
prunus_persica	Prupe.4G158000_v2.0.a1 Prupe.4G158000_v2.0.a1
pyrus_communis	pycom03g19620 pycom03g19750 pycom06g03840 pycom11g23530
rosa_chinensis	RchiOBHm_Chr5g0029911 RchiOBHm_Chr5g0029981
rosa_laevigata	RLG00000033201 RLG00000033206
rosa_multiflora	Rmu_sc0003368.1_g000014 Rmu_sc0004542.1_g000020 Rmu_sc0021047.1_g000001
rosa_roxburghii	Rroxscaffold_1G00050050
rosa_rugosa	Rorug05G0115300 Rorug05G0115400 Rorug05G0115500 Rorug05G0115600 Rorug05G0115700 Rorug05G0115800 Rorug05G0115900 Rorug05G0116000 Rorug05G0116100 Rorug05G0116200
rosa_samantha	Rh5AG208900 Rh5AG209100 Rh5BG207700 Rh5CG231100 Rh5CG231800 Rh5DG211900 Rh5DG212500
rosa_wichuraiana	Rw5G019010 Rw5G019050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1248
Acc16I	TGCGCA	1 cut(s)	1039
AccB1I	GGYRCC	2 cut(s)	1711, 2123
AccI	GTMKAC	3 cut(s)	915, 1419, 1758
AccII	CGCG	1 cut(s)	607
AciI	CCGC	1 cut(s)	670
AclI	AACGTT	2 cut(s)	247, 2087
AclWI	GGATC	4 cut(s)	289, 856, 1889, 1902
AcoI	YGGCCR	4 cut(s)	442, 933, 1493, 1644
AcsI	RAATTY	5 cut(s)	147, 316, 1322, 1876, 1913
AcyI	GRCGYC	1 cut(s)	947
AfaI	GTAC	8 cut(s)	73, 222, 523, 632, 677, 1240, 1427, 1779
AfeI	AGCGCT	1 cut(s)	1077
AfiI	CCNNNNNNNGG	3 cut(s)	874, 908, 2198
AflII	CTTAAG	2 cut(s)	121, 626
AflIII	ACRYGT	1 cut(s)	1596
AgeI	ACCGGT	1 cut(s)	524
AhlI	ACTAGT	1 cut(s)	106
AjuI	GAANNNNNNNTTGG	4 cut(s)	382, 414, 1218, 1250
AleI	CACNNNNGTG	1 cut(s)	1595
Alw26I	GTCTC	1 cut(s)	1106
AlwI	GGATC	4 cut(s)	289, 856, 1889, 1902
Aor51HI	AGCGCT	1 cut(s)	1077
ApeKI	GCWGC	5 cut(s)	657, 1288, 1688, 2027, 2162
ApoI	RAATTY	5 cut(s)	147, 316, 1322, 1876, 1913
ArsI	GACNNNNNNTTYG	4 cut(s)	301, 333, 1740, 1772
AsiGI	ACCGGT	1 cut(s)	524
Asp700I	GAANNNNTTC	2 cut(s)	773, 2099
AspA2I	CCTAGG	1 cut(s)	1105
AspLEI	GCGC	6 cut(s)	607, 609, 1040, 1078, 1855, 1910
AspS9I	GGNCC	8 cut(s)	367, 476, 584, 920, 1986, 2050, 2080, 2141
AsuC2I	CCSGG	3 cut(s)	254, 583, 2128
AsuHPI	GGTGA	3 cut(s)	276, 829, 1235
AsuII	TTCGAA	1 cut(s)	1976
AsuNHI	GCTAGC	1 cut(s)	170
AvaII	GGWCC	4 cut(s)	367, 476, 2050, 2080
AvrII	CCTAGG	1 cut(s)	1105
BaeI	ACNNNNGTAYC	2 cut(s)	505, 538
BalI	TGGCCA	3 cut(s)	444, 1495, 1646
BamHI	GGATCC	1 cut(s)	1894
BanI	GGYRCC	2 cut(s)	1711, 2123
BanII	GRGCYC	1 cut(s)	470
BarI	GAAGNNNNNNTAC	2 cut(s)	1957, 1989
BauI	CACGAG	2 cut(s)	1547, 1683
BbsI	GAAGAC	2 cut(s)	434, 733
BbvI	GCAGC	5 cut(s)	669, 1300, 1675, 2014, 2174
BccI	CCATC	6 cut(s)	434, 487, 1070, 1259, 2152, 2187
BcgI	CGANNNNNNTGC	4 cut(s)	1298, 1332, 1582, 1616
BciVI	GTATCC	1 cut(s)	1697
BcnI	CCSGG	3 cut(s)	254, 583, 2128
BcoDI	GTCTC	1 cut(s)	1106
BcuI	ACTAGT	1 cut(s)	106
BfaI	CTAG	5 cut(s)	107, 171, 327, 1073, 1106
BfmI	CTRYAG	4 cut(s)	514, 1011, 1759, 1998
BfoI	RGCGCY	2 cut(s)	1079, 1911
BfrI	CTTAAG	2 cut(s)	121, 626
BfuI	GTATCC	1 cut(s)	1697
BglI	GCCNNNNNGGC	1 cut(s)	1501
BglII	AGATCT	1 cut(s)	1626
BisI	GCNGC	5 cut(s)	658, 1289, 1689, 2028, 2163
BlnI	CCTAGG	1 cut(s)	1105
BlsI	GCNGC	5 cut(s)	659, 1290, 1690, 2029, 2164
BmcAI	AGTACT	1 cut(s)	632
Bme1390I	CCNGG	3 cut(s)	254, 583, 2128
Bme18I	GGWCC	4 cut(s)	367, 476, 2050, 2080
BmgT120I	GGNCC	8 cut(s)	367, 476, 584, 920, 1986, 2050, 2080, 2141
BmiI	GGNNCC	6 cut(s)	1270, 1713, 1896, 2052, 2082, 2125
BmrFI	CCNGG	3 cut(s)	254, 583, 2128
BmsI	GCATC	3 cut(s)	538, 1025, 1177
BmtI	GCTAGC	1 cut(s)	174
BpiI	GAAGAC	2 cut(s)	434, 733
BpmI	CTGGAG	3 cut(s)	162, 878, 1802
Bpu10I	CCTNAGC	1 cut(s)	1982
Bpu14I	TTCGAA	1 cut(s)	1976
BpuEI	CTTGAG	2 cut(s)	484, 486
BpuMI	CCSGG	3 cut(s)	254, 583, 2128
BsaBI	GATNNNNATC	3 cut(s)	594, 1611, 2178
BsaHI	GRCGYC	1 cut(s)	947
BsaI	GGTCTC	1 cut(s)	1106
BsaJI	CCNNGG	2 cut(s)	88, 1105
BsaWI	WCCGGW	1 cut(s)	524
Bsc4I	CCNNNNNNNGG	3 cut(s)	874, 908, 2198
Bse118I	RCCGGY	2 cut(s)	212, 524
Bse1I	ACTGG	4 cut(s)	145, 861, 1785, 2104
Bse3DI	GCAATG	1 cut(s)	609
Bse8I	GATNNNNATC	3 cut(s)	594, 1611, 2178
BseDI	CCNNGG	2 cut(s)	88, 1105
BseGI	GGATG	6 cut(s)	706, 1040, 1135, 1360, 1391, 2179
BseJI	GATNNNNATC	3 cut(s)	594, 1611, 2178
BseLI	CCNNNNNNNGG	3 cut(s)	874, 908, 2198
BseMI	GCAATG	1 cut(s)	609
BseMII	CTCAG	2 cut(s)	8, 1826
BseNI	ACTGG	4 cut(s)	145, 861, 1785, 2104
BsePI	GCGCGC	1 cut(s)	605
BseRI	GAGGAG	1 cut(s)	370
BseXI	GCAGC	5 cut(s)	669, 1300, 1675, 2014, 2174
Bsh1236I	CGCG	1 cut(s)	607
BshNI	GGYRCC	2 cut(s)	1711, 2123
BshTI	ACCGGT	1 cut(s)	524
BsiSI	CCGG	5 cut(s)	213, 253, 525, 582, 2127
BslFI	GGGAC	2 cut(s)	269, 901
BslI	CCNNNNNNNGG	3 cut(s)	874, 908, 2198
BsmAI	GTCTC	1 cut(s)	1106
BsmFI	GGGAC	2 cut(s)	269, 901
BsmI	GAATGC	1 cut(s)	1254
Bso31I	GGTCTC	1 cut(s)	1106
Bsp119I	TTCGAA	1 cut(s)	1976
Bsp1286I	GDGCHC	1 cut(s)	470
Bsp143I	GATC	5 cut(s)	281, 687, 861, 1626, 1894
BspACI	CCGC	1 cut(s)	670
BspCNI	CTCAG	2 cut(s)	9, 1825
BspFNI	CGCG	1 cut(s)	607
BspLI	GGNNCC	6 cut(s)	1270, 1713, 1896, 2052, 2082, 2125
BspMAI	CTGCAG	1 cut(s)	2002
BspOI	GCTAGC	1 cut(s)	174
BspPI	GGATC	4 cut(s)	289, 856, 1889, 1902
BspT104I	TTCGAA	1 cut(s)	1976
BspT107I	GGYRCC	2 cut(s)	1711, 2123
BspTI	CTTAAG	2 cut(s)	121, 626
BspTNI	GGTCTC	1 cut(s)	1106
BsrDI	GCAATG	1 cut(s)	609
BsrFI	RCCGGY	2 cut(s)	212, 524
BsrI	ACTGG	4 cut(s)	145, 861, 1785, 2104
BssAI	RCCGGY	2 cut(s)	212, 524
BssECI	CCNNGG	2 cut(s)	88, 1105
BssHII	GCGCGC	1 cut(s)	605
BssMI	GATC	5 cut(s)	281, 687, 861, 1626, 1894
BssNI	GRCGYC	1 cut(s)	947
BssSI	CACGAG	2 cut(s)	1547, 1683
BssT1I	CCWWGG	2 cut(s)	88, 1105
Bst2BI	CACGAG	2 cut(s)	1547, 1683
Bst4CI	ACNGT	4 cut(s)	313, 841, 1243, 2237
Bst6I	CTCTTC	4 cut(s)	450, 1088, 1920, 2031
BstACI	GRCGYC	1 cut(s)	947
BstAFI	CTTAAG	2 cut(s)	121, 626
BstBI	TTCGAA	1 cut(s)	1976
BstC8I	GCNNGC	5 cut(s)	95, 168, 172, 607, 647
BstDEI	CTNAG	6 cut(s)	17, 432, 985, 1812, 1888, 1982
BstENI	CCTNNNNNAGG	2 cut(s)	906, 2196
BstF5I	GGATG	6 cut(s)	706, 1040, 1135, 1360, 1391, 2179
BstFNI	CGCG	1 cut(s)	607
BstH2I	RGCGCY	2 cut(s)	1079, 1911
BstHHI	GCGC	6 cut(s)	607, 609, 1040, 1078, 1855, 1910
BstKTI	GATC	5 cut(s)	284, 690, 864, 1629, 1897
BstMAI	GTCTC	1 cut(s)	1106
BstMBI	GATC	5 cut(s)	281, 687, 861, 1626, 1894
BstMWI	GCNNNNNNNGC	3 cut(s)	611, 1501, 1652
BstNSI	RCATGY	2 cut(s)	649, 1859
BstSCI	CCNGG	3 cut(s)	252, 581, 2126
BstSFI	CTRYAG	4 cut(s)	514, 1011, 1759, 1998
BstUI	CGCG	1 cut(s)	607
BstV1I	GCAGC	5 cut(s)	669, 1300, 1675, 2014, 2174
BstV2I	GAAGAC	2 cut(s)	434, 733
BstX2I	RGATCY	3 cut(s)	861, 1626, 1894
BstYI	RGATCY	3 cut(s)	861, 1626, 1894
BsuI	GTATCC	1 cut(s)	1697
BtgZI	GCGATG	1 cut(s)	1042
BtsCI	GGATG	6 cut(s)	706, 1040, 1135, 1360, 1391, 2179
BtsI	GCAGTG	1 cut(s)	1957
BtsIMutI	CAGTG	3 cut(s)	854, 1798, 1957
Cac8I	GCNNGC	5 cut(s)	95, 168, 172, 607, 647
CfoI	GCGC	6 cut(s)	607, 609, 1040, 1078, 1855, 1910
Cfr10I	RCCGGY	2 cut(s)	212, 524
Cfr13I	GGNCC	8 cut(s)	367, 476, 584, 920, 1986, 2050, 2080, 2141
CseI	GACGC	2 cut(s)	738, 955
Csp6I	GTAC	8 cut(s)	72, 221, 522, 631, 676, 1239, 1426, 1778
CspAI	ACCGGT	1 cut(s)	524
CspCI	CAANNNNNGTGG	2 cut(s)	854, 889
CviQI	GTAC	8 cut(s)	72, 221, 522, 631, 676, 1239, 1426, 1778
DdeI	CTNAG	6 cut(s)	17, 432, 985, 1812, 1888, 1982
DpnI	GATC	5 cut(s)	283, 689, 863, 1628, 1896
DpnII	GATC	5 cut(s)	281, 687, 861, 1626, 1894
EaeI	YGGCCR	4 cut(s)	442, 933, 1493, 1644
Eam1104I	CTCTTC	4 cut(s)	450, 1088, 1920, 2031
EarI	CTCTTC	4 cut(s)	450, 1088, 1920, 2031
Eco130I	CCWWGG	2 cut(s)	88, 1105
Eco24I	GRGCYC	1 cut(s)	470
Eco31I	GGTCTC	1 cut(s)	1106
Eco32I	GATATC	2 cut(s)	1582, 2176
Eco47I	GGWCC	4 cut(s)	367, 476, 2050, 2080
Eco47III	AGCGCT	1 cut(s)	1077
EcoNI	CCTNNNNNAGG	2 cut(s)	906, 2196
EcoO109I	RGGNCCY	1 cut(s)	367
EcoRI	GAATTC	1 cut(s)	1913
EcoRV	GATATC	2 cut(s)	1582, 2176
EcoT14I	CCWWGG	2 cut(s)	88, 1105
EcoT38I	GRGCYC	1 cut(s)	470
ErhI	CCWWGG	2 cut(s)	88, 1105
FalI	AAGNNNNNCTT	2 cut(s)	1154, 1186
FaqI	GGGAC	2 cut(s)	269, 901
FauNDI	CATATG	1 cut(s)	451
FblI	GTMKAC	3 cut(s)	915, 1419, 1758
Fnu4HI	GCNGC	5 cut(s)	658, 1289, 1689, 2028, 2163
FokI	GGATG	6 cut(s)	713, 1047, 1142, 1347, 1398, 2166
FriOI	GRGCYC	1 cut(s)	470
Fsp4HI	GCNGC	5 cut(s)	658, 1289, 1689, 2028, 2163
FspBI	CTAG	5 cut(s)	107, 171, 327, 1073, 1106
FspI	TGCGCA	1 cut(s)	1039
GlaI	GCGC	6 cut(s)	606, 608, 1039, 1077, 1854, 1909
GluI	GCNGC	5 cut(s)	658, 1289, 1689, 2028, 2163
GsuI	CTGGAG	3 cut(s)	162, 878, 1802
HaeII	RGCGCY	2 cut(s)	1079, 1911
HapII	CCGG	5 cut(s)	213, 253, 525, 582, 2127
HgaI	GACGC	2 cut(s)	738, 955
HhaI	GCGC	6 cut(s)	607, 609, 1040, 1078, 1855, 1910
Hin1I	GRCGYC	1 cut(s)	947
Hin6I	GCGC	6 cut(s)	605, 607, 1038, 1076, 1853, 1908
HinP1I	GCGC	6 cut(s)	605, 607, 1038, 1076, 1853, 1908
HincII	GTYRAC	2 cut(s)	138, 302
HindII	GTYRAC	2 cut(s)	138, 302
HindIII	AAGCTT	3 cut(s)	124, 1558, 1979
HinfI	GANTC	3 cut(s)	1000, 1553, 2153
HpaII	CCGG	5 cut(s)	213, 253, 525, 582, 2127
HphI	GGTGA	3 cut(s)	276, 829, 1235
Hpy166II	GTNNAC	9 cut(s)	138, 302, 532, 696, 916, 1167, 1375, 1420, 1759
Hpy188I	TCNGA	6 cut(s)	18, 476, 685, 821, 1633, 1870
Hpy188III	TCNNGA	5 cut(s)	503, 1319, 1547, 1844, 2168
Hpy8I	GTNNAC	9 cut(s)	138, 302, 532, 696, 916, 1167, 1375, 1420, 1759
Hpy99I	CGWCG	2 cut(s)	340, 499
HpyCH4III	ACNGT	4 cut(s)	313, 841, 1243, 2237
HpyCH4IV	ACGT	3 cut(s)	247, 1596, 2087
HpyCH4V	TGCA	8 cut(s)	238, 551, 645, 1357, 1724, 1826, 2000, 2162
HpyF10VI	GCNNNNNNNGC	3 cut(s)	611, 1501, 1652
HpyF3I	CTNAG	6 cut(s)	17, 432, 985, 1812, 1888, 1982
HpySE526I	ACGT	3 cut(s)	247, 1596, 2087
Hsp92I	GRCGYC	1 cut(s)	947
HspAI	GCGC	6 cut(s)	605, 607, 1038, 1076, 1853, 1908
Kzo9I	GATC	5 cut(s)	281, 687, 861, 1626, 1894
LmnI	GCTCC	1 cut(s)	383
Lsp1109I	GCAGC	5 cut(s)	669, 1300, 1675, 2014, 2174
LweI	GCATC	3 cut(s)	538, 1025, 1177
MaeI	CTAG	5 cut(s)	107, 171, 327, 1073, 1106
MaeII	ACGT	3 cut(s)	247, 1596, 2087
MaeIII	GTNAC	5 cut(s)	109, 248, 562, 835, 943
MalI	GATC	5 cut(s)	283, 689, 863, 1628, 1896
MboI	GATC	5 cut(s)	281, 687, 861, 1626, 1894
MfeI	CAATTG	2 cut(s)	159, 412
MflI	RGATCY	3 cut(s)	861, 1626, 1894
MhlI	GDGCHC	1 cut(s)	470
MlsI	TGGCCA	3 cut(s)	444, 1495, 1646
MluNI	TGGCCA	3 cut(s)	444, 1495, 1646
MlyI	GAGTC	1 cut(s)	1009
MmeI	TCCRAC	1 cut(s)	1467
Mox20I	TGGCCA	3 cut(s)	444, 1495, 1646
MroXI	GAANNNNTTC	2 cut(s)	773, 2099
MscI	TGGCCA	3 cut(s)	444, 1495, 1646
MseI	TTAA	6 cut(s)	122, 129, 627, 977, 1284, 1617
MslI	CAYNNNNRTG	4 cut(s)	38, 450, 941, 1595
Msp20I	TGGCCA	3 cut(s)	444, 1495, 1646
MspA1I	CMGCKG	1 cut(s)	297
MspCI	CTTAAG	2 cut(s)	121, 626
MspI	CCGG	5 cut(s)	213, 253, 525, 582, 2127
MspR9I	CCNGG	3 cut(s)	254, 583, 2128
MunI	CAATTG	2 cut(s)	159, 412
Mva1269I	GAATGC	1 cut(s)	1254
MvnI	CGCG	1 cut(s)	607
MwoI	GCNNNNNNNGC	3 cut(s)	611, 1501, 1652
NciI	CCSGG	3 cut(s)	254, 583, 2128
NdeI	CATATG	1 cut(s)	451
NdeII	GATC	5 cut(s)	281, 687, 861, 1626, 1894
NheI	GCTAGC	1 cut(s)	170
NlaIV	GGNNCC	6 cut(s)	1270, 1713, 1896, 2052, 2082, 2125
NmuCI	GTSAC	2 cut(s)	835, 943
NsbI	TGCGCA	1 cut(s)	1039
NspI	RCATGY	2 cut(s)	649, 1859
NspV	TTCGAA	1 cut(s)	1976
OliI	CACNNNNGTG	1 cut(s)	1595
PaeI	GCATGC	1 cut(s)	649
PauI	GCGCGC	1 cut(s)	605
PctI	GAATGC	1 cut(s)	1254
PdmI	GAANNNNTTC	2 cut(s)	773, 2099
PfeI	GAWTC	2 cut(s)	1553, 2153
PflFI	GACNNNGTC	1 cut(s)	833
PinAI	ACCGGT	1 cut(s)	524
PkrI	GCNGC	5 cut(s)	659, 1290, 1690, 2029, 2164
PleI	GAGTC	1 cut(s)	1008
PpsI	GAGTC	1 cut(s)	1008
PpuMI	RGGWCCY	1 cut(s)	367
PsiI	TTATAA	1 cut(s)	1248
Psp1406I	AACGTT	2 cut(s)	247, 2087
Psp5II	RGGWCCY	1 cut(s)	367
PspN4I	GGNNCC	6 cut(s)	1270, 1713, 1896, 2052, 2082, 2125
PspPI	GGNCC	8 cut(s)	367, 476, 584, 920, 1986, 2050, 2080, 2141
PspPPI	RGGWCCY	1 cut(s)	367
PstI	CTGCAG	1 cut(s)	2002
PsuI	RGATCY	3 cut(s)	861, 1626, 1894
PsyI	GACNNNGTC	1 cut(s)	833
PteI	GCGCGC	1 cut(s)	605
PvuII	CAGCTG	1 cut(s)	297
RsaI	GTAC	8 cut(s)	73, 222, 523, 632, 677, 1240, 1427, 1779
RsaNI	GTAC	8 cut(s)	72, 221, 522, 631, 676, 1239, 1426, 1778
RseI	CAYNNNNRTG	4 cut(s)	38, 450, 941, 1595
SaqAI	TTAA	6 cut(s)	122, 129, 627, 977, 1284, 1617
SatI	GCNGC	5 cut(s)	658, 1289, 1689, 2028, 2163
Sau3AI	GATC	5 cut(s)	281, 687, 861, 1626, 1894
Sau96I	GGNCC	8 cut(s)	367, 476, 584, 920, 1986, 2050, 2080, 2141
ScaI	AGTACT	1 cut(s)	632
SchI	GAGTC	1 cut(s)	1009
ScrFI	CCNGG	3 cut(s)	254, 583, 2128
SduI	GDGCHC	1 cut(s)	470
SfaNI	GCATC	3 cut(s)	538, 1025, 1177
SfcI	CTRYAG	4 cut(s)	514, 1011, 1759, 1998
SfuI	TTCGAA	1 cut(s)	1976
SinI	GGWCC	4 cut(s)	367, 476, 2050, 2080
SmiMI	CAYNNNNRTG	4 cut(s)	38, 450, 941, 1595
SmlI	CTYRAG	4 cut(s)	121, 463, 501, 626
SmoI	CTYRAG	4 cut(s)	121, 463, 501, 626
SpeI	ACTAGT	1 cut(s)	106
SphI	GCATGC	1 cut(s)	649
SsiI	CCGC	1 cut(s)	670
SspMI	CTAG	5 cut(s)	107, 171, 327, 1073, 1106
StyD4I	CCNGG	3 cut(s)	252, 581, 2126
StyI	CCWWGG	2 cut(s)	88, 1105
TaaI	ACNGT	4 cut(s)	313, 841, 1243, 2237
TaiI	ACGT	3 cut(s)	250, 1599, 2090
TaqI	TCGA	4 cut(s)	1976, 2016, 2063, 2147
TatI	WGTACW	4 cut(s)	630, 675, 1425, 1777
TfiI	GAWTC	2 cut(s)	1553, 2153
Tru1I	TTAA	6 cut(s)	122, 129, 627, 977, 1284, 1617
Tru9I	TTAA	6 cut(s)	122, 129, 627, 977, 1284, 1617
TscAI	CASTG	3 cut(s)	861, 1798, 1964
TseFI	GTSAC	2 cut(s)	835, 943
TseI	GCWGC	5 cut(s)	657, 1288, 1688, 2027, 2162
Tsp45I	GTSAC	2 cut(s)	835, 943
TspDTI	ATGAA	6 cut(s)	41, 175, 468, 1898, 1938, 2145
TspGWI	ACGGA	3 cut(s)	280, 713, 1398
TspRI	CASTG	3 cut(s)	861, 1798, 1964
Tth111I	GACNNNGTC	1 cut(s)	833
Vha464I	CTTAAG	2 cut(s)	121, 626
VpaK11BI	GGWCC	4 cut(s)	367, 476, 2050, 2080
XagI	CCTNNNNNAGG	2 cut(s)	906, 2196
XapI	RAATTY	5 cut(s)	147, 316, 1322, 1876, 1913
XceI	RCATGY	2 cut(s)	649, 1859
XcmI	CCANNNNNNNNNTGG	1 cut(s)	348
XmaJI	CCTAGG	1 cut(s)	1105
XmiI	GTMKAC	3 cut(s)	915, 1419, 1758
XmnI	GAANNNNTTC	2 cut(s)	773, 2099
XspI	CTAG	5 cut(s)	107, 171, 327, 1073, 1106
ZrmI	AGTACT	1 cut(s)	632

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.