Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0002164

Physical Location & Seq

Genomic Coordinates Forward (+)

33695178 .. 33698830

Gene Structure 3653 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1249200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2061 bp

ATGCTTGTTGCTTCCTTTGCCGTGTATTTGTTTCTGTGCTTTTCTTTCGTCTCTTGCTTTTGCTGTGTTTCTCTGCTCTTTCTTTGTTTTGTTTTTCTTTTCTCCTCTGCCGTCTCTCTTCTCTGCTGTGTTCTGCCGTCTCTCCGTTGTCCTCTTTTTCTGTCTCCCGTTTTCTGTGTCCCTTTGCAGTATGTTGCTCCTCTTCCCGTTTACCATTCAATATTTATCAATTGTGGAGGGAGGAACACGAAGTTTGATGGCCATACATATGAAGAGGACTTGAGCCCTTTCGGACCATCACACTTTGTTTCGTCGGCTCAAGAATGGGGCTACAGCAGTACCGGTGTGGACACGAGCAACCCTGATGCAAATTACATCGCAACATCTTTGAACATTACCGGGCCAGATTTATATCAAACAGCGCGCATTGCCCCTCTTTCACTTAAGTACTATGGCCTTTGCATGCTCAAAGGCAACTACAAAGTGCGGCTCTACTTTTCCGAGATCATGTTCACGGATGACCAAACATTTAGCAGTCTTGGAAAACGCTTGTTTGACATCTCAATTCAAGGTAACTTGGTTTTGAAAGACTTCAACATTATGGAAGAAGCAAGAGGTGTCGGTAAGGGCATTGTCAGAGAGTTTGACAATGTCACCGTTATTGGTAGCACACTGGAGATCCACCTATATTGGGCAGGAAAAGGGACGACTGCCATTCCTTATACAGGTGTCTACGGGCCTCTCATATCAGCCATTAGTGTGACGCCAAACTTTGAACCTCCAAAAAAAACTTTAAGCTACTTAGAGCGTATTAGGAGTCATGTGCCTATAGGCACAATGGTAGGAATTGGATGCGCAATAGCAGTCGTCATCATCGCCATCGTATTACTAGCGCTCGTTATTCTTTGGAAGAGAGGTCTCCTAGGACGACAAAAAACATTGGAGGATGATTTGAAAGGTGTGGAACTACAAACTCGCAAGTTTACCTTTACACAACTCAAAGATGCTACAACCAACTTTGACCAAGCCAATAAGATTGGTGAAGGTGGTTTTGGTACTGTTTATAAGGGCATTCTTGTAGATGGAACCCTGATTGCTGTTAAGCAGCTTTCTTCCAAGTCAAAGCAAGGAAATCGGGAATTTGTGAATGAGATAGGCATGATTTCTGCTTTGCAACATCCTCATCTTGTAAACCTATATGGATGTTGTATTGAAGGAAATCAACTCTACCTTGTCTACGAGTACATGGAAAACAATAGCGTTTCTCATGCTTTGTTTGGGGCAGAAGAAAGTCAGTTGAAGTTGGATTGGCCAACGAGGCATAGGATATGTGTTGGCATAGCAAAAGGTTTGGCTTATCTTCACGAGGAATCAAGCTTGAAGGTTATCCATAGAGATATCAAGGCCACGAACGTGTTGCCTGATAGACATCTTAACCCGAAGATATCGGACTTTGGGTTGGCCAAACTTGCTGAAGAGGATAAAACACATATAAGCACCCGTGCTGCTGGAACTTTTGGATACATGGCACCAGAATATGCACTTCGGGGTCATCTCACAGACAAAGCAGATGTCTACAGCTTTGGAATTGTTGTGCTGGAGATTATCAGTGGGAAGAAGAACTCAACTCAGCAGACAAATGCAGAAGGATTTTATATTCTTGATTGGGCGCATGTTCTCAAATCAGAAGGGAATTTGATGAACTTAGTGGATCCAAAGTTGAGCTCCGAATTCAACAATGAAGAGATGTTGGCTACAATCAATGTAGCTCTCCACTGCTGTAATTCCACTTCGAAGCTTAGGCCCACTATGTCTGCAGTTGTGAATATGCTTGAAGGTAAGGCTGCTGTTGAAGAGTTTGTCTTGGACCCCAATCCCTCGACAAGTGATAACATGGACCCAACGTTGAAGAAACTTTTCCAGTTTTGTTTGAGAGGGGGTGCCGGGTCAGTTGAAGGGCCATCGAATGATTCATTTGCAGCTCTTGATGATATCCATCCATACAATCCTCATTCAGGTCGCCAAGACGATAGAAAAGGGAGAAAATGGCACAGTTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_1 LRR_8 Malectin Pkinase Pkinase_Tyr

Protein Analysis

687

Amino Acids

75.87

Weight (kDa)

6.45

Isoelectric Point (pI)

28.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Malectin	PF11721	74 - 252	3.1e-37	Malectin domain
Pkinase	PF00069	341 - 609	2.2e-48	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	343 - 611	5.3e-49	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002164)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53430 AT1G53430 AT1G53440
fragaria_vesca	FvH4_3g17940
malus_domestica	MD03G1247100.v1.1 MD03G1248200.v1.1 MD03G1248300.v1.1 MD03G1249100.v1.1 MD03G1249200.v1.1 MD11G1267500.v1.1
prunus_persica	Prupe.4G158000_v2.0.a1 Prupe.4G158000_v2.0.a1
pyrus_communis	pycom03g19620 pycom03g19750 pycom06g03840 pycom11g23530
rosa_chinensis	RchiOBHm_Chr5g0029911 RchiOBHm_Chr5g0029981
rosa_laevigata	RLG00000033201 RLG00000033206
rosa_multiflora	Rmu_sc0003368.1_g000014 Rmu_sc0004542.1_g000020 Rmu_sc0021047.1_g000001
rosa_roxburghii	Rroxscaffold_1G00050050
rosa_rugosa	Rorug05G0115300 Rorug05G0115400 Rorug05G0115500 Rorug05G0115600 Rorug05G0115700 Rorug05G0115800 Rorug05G0115900 Rorug05G0116000 Rorug05G0116100 Rorug05G0116200
rosa_samantha	Rh5AG208900 Rh5AG209100 Rh5BG207700 Rh5CG231100 Rh5CG231800 Rh5DG211900 Rh5DG212500
rosa_wichuraiana	Rw5G019010 Rw5G019050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1065
Acc16I	TGCGCA	1 cut(s)	856
AccB1I	GGYRCC	2 cut(s)	1528, 1940
AccI	GTMKAC	3 cut(s)	732, 1236, 1575
AccII	CGCG	1 cut(s)	424
AciI	CCGC	1 cut(s)	487
AclI	AACGTT	1 cut(s)	1904
AclWI	GGATC	3 cut(s)	673, 1706, 1719
AcoI	YGGCCR	3 cut(s)	259, 1310, 1461
AcsI	RAATTY	3 cut(s)	1139, 1693, 1730
AcuI	CTGAAG	1 cut(s)	1494
AcyI	GRCGYC	1 cut(s)	764
AfaI	GTAC	4 cut(s)	340, 449, 1057, 1244
AfeI	AGCGCT	1 cut(s)	894
AfiI	CCNNNNNNNGG	3 cut(s)	691, 725, 2015
AflII	CTTAAG	1 cut(s)	443
AflIII	ACRYGT	1 cut(s)	1413
AgeI	ACCGGT	1 cut(s)	341
AjuI	GAANNNNNNNTTGG	2 cut(s)	1035, 1067
AleI	CACNNNNGTG	1 cut(s)	1412
AluBI	AGCT	8 cut(s)	798, 1108, 1377, 1581, 1725, 1769, 1798, 1982
AluI	AGCT	8 cut(s)	798, 1108, 1377, 1581, 1725, 1769, 1798, 1982
Alw21I	GWGCWC	1 cut(s)	1727
Alw26I	GTCTC	5 cut(s)	55, 118, 144, 168, 923
AlwI	GGATC	3 cut(s)	673, 1706, 1719
Aor51HI	AGCGCT	1 cut(s)	894
AoxI	GGCC	9 cut(s)	259, 401, 454, 737, 1310, 1404, 1461, 1802, 1958
ApeKI	GCWGC	4 cut(s)	1105, 1505, 1844, 1979
ApoI	RAATTY	3 cut(s)	1139, 1693, 1730
ArsI	GACNNNNNNTTYG	2 cut(s)	1557, 1589
AsiGI	ACCGGT	1 cut(s)	341
Asp700I	GAANNNNTTC	2 cut(s)	590, 1916
AspA2I	CCTAGG	1 cut(s)	922
AspLEI	GCGC	5 cut(s)	424, 426, 857, 895, 1672
AspS9I	GGNCC	7 cut(s)	293, 401, 737, 1803, 1867, 1897, 1958
AsuC2I	CCSGG	2 cut(s)	400, 1945
AsuHPI	GGTGA	2 cut(s)	646, 1052
AsuII	TTCGAA	1 cut(s)	1793
AvaII	GGWCC	3 cut(s)	293, 1867, 1897
AvrII	CCTAGG	1 cut(s)	922
BaeI	ACNNNNGTAYC	2 cut(s)	322, 355
BalI	TGGCCA	3 cut(s)	261, 1312, 1463
BamHI	GGATCC	1 cut(s)	1711
BanI	GGYRCC	2 cut(s)	1528, 1940
BanII	GRGCYC	2 cut(s)	287, 1727
BarI	GAAGNNNNNNTAC	2 cut(s)	1774, 1806
BauI	CACGAG	2 cut(s)	352, 1364
Bbv12I	GWGCWC	1 cut(s)	1727
BbvI	GCAGC	4 cut(s)	1117, 1492, 1831, 1991
BccI	CCATC	6 cut(s)	251, 304, 887, 1076, 1969, 2004
BceAI	ACGGC	3 cut(s)	5, 95, 121
BcgI	CGANNNNNNTGC	4 cut(s)	1115, 1149, 1399, 1433
BciVI	GTATCC	1 cut(s)	1514
BcnI	CCSGG	2 cut(s)	400, 1945
BcoDI	GTCTC	5 cut(s)	55, 118, 144, 168, 923
BfaI	CTAG	2 cut(s)	890, 923
BfmI	CTRYAG	4 cut(s)	331, 828, 1576, 1815
BfoI	RGCGCY	1 cut(s)	896
BfrI	CTTAAG	1 cut(s)	443
BfuI	GTATCC	1 cut(s)	1514
BglI	GCCNNNNNGGC	1 cut(s)	1318
BisI	GCNGC	5 cut(s)	488, 1106, 1506, 1845, 1980
BlnI	CCTAGG	1 cut(s)	922
BlsI	GCNGC	5 cut(s)	489, 1107, 1507, 1846, 1981
BmcAI	AGTACT	1 cut(s)	449
Bme1390I	CCNGG	2 cut(s)	400, 1945
Bme18I	GGWCC	3 cut(s)	293, 1867, 1897
BmgT120I	GGNCC	7 cut(s)	293, 401, 737, 1803, 1867, 1897, 1958
BmiI	GGNNCC	6 cut(s)	1087, 1530, 1713, 1869, 1899, 1942
BmrFI	CCNGG	2 cut(s)	400, 1945
BmsI	GCATC	3 cut(s)	355, 842, 994
BpmI	CTGGAG	2 cut(s)	695, 1619
Bpu10I	CCTNAGC	1 cut(s)	1799
Bpu14I	TTCGAA	1 cut(s)	1793
BpuEI	CTTGAG	2 cut(s)	301, 303
BpuMI	CCSGG	2 cut(s)	400, 1945
BsaBI	GATNNNNATC	3 cut(s)	411, 1428, 1995
BsaHI	GRCGYC	1 cut(s)	764
BsaI	GGTCTC	1 cut(s)	923
BsaJI	CCNNGG	1 cut(s)	922
BsaWI	WCCGGW	1 cut(s)	341
Bsc4I	CCNNNNNNNGG	3 cut(s)	691, 725, 2015
Bse118I	RCCGGY	1 cut(s)	341
Bse1I	ACTGG	2 cut(s)	678, 1921
Bse3DI	GCAATG	1 cut(s)	426
Bse8I	GATNNNNATC	3 cut(s)	411, 1428, 1995
BseDI	CCNNGG	1 cut(s)	922
BseGI	GGATG	6 cut(s)	523, 857, 952, 1177, 1208, 1996
BseJI	GATNNNNATC	3 cut(s)	411, 1428, 1995
BseLI	CCNNNNNNNGG	3 cut(s)	691, 725, 2015
BseMI	GCAATG	1 cut(s)	426
BseMII	CTCAG	1 cut(s)	1643
BseNI	ACTGG	2 cut(s)	678, 1921
BsePI	GCGCGC	1 cut(s)	422
BseRI	GAGGAG	2 cut(s)	94, 189
BseXI	GCAGC	4 cut(s)	1117, 1492, 1831, 1991
Bsh1236I	CGCG	1 cut(s)	424
BshFI	GGCC	9 cut(s)	261, 403, 456, 739, 1312, 1406, 1463, 1804, 1960
BshNI	GGYRCC	2 cut(s)	1528, 1940
BshTI	ACCGGT	1 cut(s)	341
BsiHKAI	GWGCWC	1 cut(s)	1727
BsiSI	CCGG	3 cut(s)	342, 399, 1944
BslFI	GGGAC	2 cut(s)	164, 718
BslI	CCNNNNNNNGG	3 cut(s)	691, 725, 2015
BsmAI	GTCTC	5 cut(s)	55, 118, 144, 168, 923
BsmBI	CGTCTC	3 cut(s)	55, 118, 144
BsmFI	GGGAC	2 cut(s)	164, 718
BsmI	GAATGC	1 cut(s)	1071
BsnI	GGCC	9 cut(s)	261, 403, 456, 739, 1312, 1406, 1463, 1804, 1960
Bso31I	GGTCTC	1 cut(s)	923
Bsp119I	TTCGAA	1 cut(s)	1793
Bsp1286I	GDGCHC	2 cut(s)	287, 1727
Bsp143I	GATC	3 cut(s)	504, 678, 1711
BspACI	CCGC	1 cut(s)	487
BspANI	GGCC	9 cut(s)	261, 403, 456, 739, 1312, 1406, 1463, 1804, 1960
BspCNI	CTCAG	1 cut(s)	1642
BspFNI	CGCG	1 cut(s)	424
BspLI	GGNNCC	6 cut(s)	1087, 1530, 1713, 1869, 1899, 1942
BspMAI	CTGCAG	1 cut(s)	1819
BspPI	GGATC	3 cut(s)	673, 1706, 1719
BspT104I	TTCGAA	1 cut(s)	1793
BspT107I	GGYRCC	2 cut(s)	1528, 1940
BspTI	CTTAAG	1 cut(s)	443
BspTNI	GGTCTC	1 cut(s)	923
BsrDI	GCAATG	1 cut(s)	426
BsrFI	RCCGGY	1 cut(s)	341
BsrI	ACTGG	2 cut(s)	678, 1921
BssAI	RCCGGY	1 cut(s)	341
BssECI	CCNNGG	1 cut(s)	922
BssHII	GCGCGC	1 cut(s)	422
BssMI	GATC	3 cut(s)	504, 678, 1711
BssNI	GRCGYC	1 cut(s)	764
BssSI	CACGAG	2 cut(s)	352, 1364
BssT1I	CCWWGG	1 cut(s)	922
Bst2BI	CACGAG	2 cut(s)	352, 1364
Bst4CI	ACNGT	3 cut(s)	658, 1060, 2054
Bst6I	CTCTTC	7 cut(s)	123, 207, 267, 905, 1470, 1737, 1848
BstACI	GRCGYC	1 cut(s)	764
BstAFI	CTTAAG	1 cut(s)	443
BstBI	TTCGAA	1 cut(s)	1793
BstC8I	GCNNGC	2 cut(s)	424, 464
BstDEI	CTNAG	4 cut(s)	802, 1629, 1705, 1799
BstENI	CCTNNNNNAGG	2 cut(s)	723, 2013
BstF5I	GGATG	6 cut(s)	523, 857, 952, 1177, 1208, 1996
BstFNI	CGCG	1 cut(s)	424
BstH2I	RGCGCY	1 cut(s)	896
BstHHI	GCGC	5 cut(s)	424, 426, 857, 895, 1672
BstKTI	GATC	3 cut(s)	507, 681, 1714
BstMAI	GTCTC	5 cut(s)	55, 118, 144, 168, 923
BstMBI	GATC	3 cut(s)	504, 678, 1711
BstMWI	GCNNNNNNNGC	4 cut(s)	17, 428, 1318, 1469
BstNSI	RCATGY	2 cut(s)	466, 1676
BstSCI	CCNGG	2 cut(s)	398, 1943
BstSFI	CTRYAG	4 cut(s)	331, 828, 1576, 1815
BstUI	CGCG	1 cut(s)	424
BstV1I	GCAGC	4 cut(s)	1117, 1492, 1831, 1991
BstX2I	RGATCY	2 cut(s)	678, 1711
BstYI	RGATCY	2 cut(s)	678, 1711
BsuI	GTATCC	1 cut(s)	1514
BsuRI	GGCC	9 cut(s)	261, 403, 456, 739, 1312, 1406, 1463, 1804, 1960
BtgZI	GCGATG	2 cut(s)	361, 859
BtsCI	GGATG	6 cut(s)	523, 857, 952, 1177, 1208, 1996
BtsI	GCAGTG	1 cut(s)	1774
BtsIMutI	CAGTG	3 cut(s)	671, 1615, 1774
Cac8I	GCNNGC	2 cut(s)	424, 464
CfoI	GCGC	5 cut(s)	424, 426, 857, 895, 1672
Cfr10I	RCCGGY	1 cut(s)	341
Cfr13I	GGNCC	7 cut(s)	293, 401, 737, 1803, 1867, 1897, 1958
CseI	GACGC	1 cut(s)	772
Csp6I	GTAC	4 cut(s)	339, 448, 1056, 1243
CspAI	ACCGGT	1 cut(s)	341
CspCI	CAANNNNNGTGG	2 cut(s)	671, 706
CviAII	CATG	9 cut(s)	463, 508, 821, 1159, 1246, 1268, 1525, 1673, 1894
CviQI	GTAC	4 cut(s)	339, 448, 1056, 1243
DdeI	CTNAG	4 cut(s)	802, 1629, 1705, 1799
DpnI	GATC	3 cut(s)	506, 680, 1713
DpnII	GATC	3 cut(s)	504, 678, 1711
EaeI	YGGCCR	3 cut(s)	259, 1310, 1461
Eam1104I	CTCTTC	7 cut(s)	123, 207, 267, 905, 1470, 1737, 1848
EarI	CTCTTC	7 cut(s)	123, 207, 267, 905, 1470, 1737, 1848
Ecl136II	GAGCTC	1 cut(s)	1725
Eco130I	CCWWGG	1 cut(s)	922
Eco24I	GRGCYC	2 cut(s)	287, 1727
Eco31I	GGTCTC	1 cut(s)	923
Eco32I	GATATC	3 cut(s)	1399, 1446, 1993
Eco47I	GGWCC	3 cut(s)	293, 1867, 1897
Eco47III	AGCGCT	1 cut(s)	894
Eco53kI	GAGCTC	1 cut(s)	1725
Eco57I	CTGAAG	1 cut(s)	1494
EcoICRI	GAGCTC	1 cut(s)	1725
EcoNI	CCTNNNNNAGG	2 cut(s)	723, 2013
EcoRI	GAATTC	1 cut(s)	1730
EcoRV	GATATC	3 cut(s)	1399, 1446, 1993
EcoT14I	CCWWGG	1 cut(s)	922
EcoT38I	GRGCYC	2 cut(s)	287, 1727
ErhI	CCWWGG	1 cut(s)	922
Esp3I	CGTCTC	3 cut(s)	55, 118, 144
FaeI	CATG	9 cut(s)	466, 511, 824, 1162, 1249, 1271, 1528, 1676, 1897
FalI	AAGNNNNNCTT	2 cut(s)	971, 1003
FaqI	GGGAC	2 cut(s)	164, 718
FatI	CATG	9 cut(s)	462, 507, 820, 1158, 1245, 1267, 1524, 1672, 1893
FauNDI	CATATG	1 cut(s)	268
FblI	GTMKAC	3 cut(s)	732, 1236, 1575
Fnu4HI	GCNGC	5 cut(s)	488, 1106, 1506, 1845, 1980
FokI	GGATG	6 cut(s)	530, 864, 959, 1164, 1215, 1983
FriOI	GRGCYC	2 cut(s)	287, 1727
Fsp4HI	GCNGC	5 cut(s)	488, 1106, 1506, 1845, 1980
FspBI	CTAG	2 cut(s)	890, 923
FspI	TGCGCA	1 cut(s)	856
GlaI	GCGC	5 cut(s)	423, 425, 856, 894, 1671
GluI	GCNGC	5 cut(s)	488, 1106, 1506, 1845, 1980
GsuI	CTGGAG	2 cut(s)	695, 1619
HaeII	RGCGCY	1 cut(s)	896
HaeIII	GGCC	9 cut(s)	261, 403, 456, 739, 1312, 1406, 1463, 1804, 1960
HapII	CCGG	3 cut(s)	342, 399, 1944
HgaI	GACGC	1 cut(s)	772
HhaI	GCGC	5 cut(s)	424, 426, 857, 895, 1672
Hin1I	GRCGYC	1 cut(s)	764
Hin1II	CATG	9 cut(s)	466, 511, 824, 1162, 1249, 1271, 1528, 1676, 1897
Hin6I	GCGC	5 cut(s)	422, 424, 855, 893, 1670
HinP1I	GCGC	5 cut(s)	422, 424, 855, 893, 1670
HindIII	AAGCTT	2 cut(s)	1375, 1796
HinfI	GANTC	3 cut(s)	817, 1370, 1970
HpaII	CCGG	3 cut(s)	342, 399, 1944
HphI	GGTGA	2 cut(s)	646, 1052
Hpy166II	GTNNAC	8 cut(s)	211, 349, 513, 733, 984, 1192, 1237, 1576
Hpy188I	TCNGA	6 cut(s)	293, 502, 638, 1450, 1687, 1729
Hpy188III	TCNNGA	5 cut(s)	320, 1136, 1364, 1661, 1985
Hpy8I	GTNNAC	8 cut(s)	211, 349, 513, 733, 984, 1192, 1237, 1576
Hpy99I	CGWCG	1 cut(s)	316
HpyAV	CCTTC	7 cut(s)	1037, 1208, 1375, 1640, 1682, 1829, 1949
HpyCH4III	ACNGT	3 cut(s)	658, 1060, 2054
HpyCH4IV	ACGT	2 cut(s)	1413, 1904
HpyCH4V	TGCA	8 cut(s)	187, 368, 462, 1174, 1541, 1643, 1817, 1979
HpyF10VI	GCNNNNNNNGC	4 cut(s)	17, 428, 1318, 1469
HpyF3I	CTNAG	4 cut(s)	802, 1629, 1705, 1799
HpySE526I	ACGT	2 cut(s)	1413, 1904
Hsp92I	GRCGYC	1 cut(s)	764
Hsp92II	CATG	9 cut(s)	466, 511, 824, 1162, 1249, 1271, 1528, 1676, 1897
HspAI	GCGC	5 cut(s)	422, 424, 855, 893, 1670
Kzo9I	GATC	3 cut(s)	504, 678, 1711
LmnI	GCTCC	2 cut(s)	202, 1730
Lsp1109I	GCAGC	4 cut(s)	1117, 1492, 1831, 1991
LweI	GCATC	3 cut(s)	355, 842, 994
MaeI	CTAG	2 cut(s)	890, 923
MaeII	ACGT	2 cut(s)	1413, 1904
MaeIII	GTNAC	3 cut(s)	572, 652, 760
MalI	GATC	3 cut(s)	506, 680, 1713
MboI	GATC	3 cut(s)	504, 678, 1711
MfeI	CAATTG	1 cut(s)	229
MflI	RGATCY	2 cut(s)	678, 1711
MhlI	GDGCHC	2 cut(s)	287, 1727
MlsI	TGGCCA	3 cut(s)	261, 1312, 1463
MluCI	AATT	9 cut(s)	229, 370, 564, 846, 1139, 1587, 1693, 1730, 1783
MluNI	TGGCCA	3 cut(s)	261, 1312, 1463
MlyI	GAGTC	1 cut(s)	826
MmeI	TCCRAC	1 cut(s)	1284
Mox20I	TGGCCA	3 cut(s)	261, 1312, 1463
MroXI	GAANNNNTTC	2 cut(s)	590, 1916
MscI	TGGCCA	3 cut(s)	261, 1312, 1463
MseI	TTAA	4 cut(s)	444, 794, 1101, 1434
MslI	CAYNNNNRTG	3 cut(s)	267, 758, 1412
Msp20I	TGGCCA	3 cut(s)	261, 1312, 1463
MspCI	CTTAAG	1 cut(s)	443
MspI	CCGG	3 cut(s)	342, 399, 1944
MspR9I	CCNGG	2 cut(s)	400, 1945
MunI	CAATTG	1 cut(s)	229
Mva1269I	GAATGC	1 cut(s)	1071
MvnI	CGCG	1 cut(s)	424
MwoI	GCNNNNNNNGC	4 cut(s)	17, 428, 1318, 1469
NciI	CCSGG	2 cut(s)	400, 1945
NdeI	CATATG	1 cut(s)	268
NdeII	GATC	3 cut(s)	504, 678, 1711
NlaIII	CATG	9 cut(s)	466, 511, 824, 1162, 1249, 1271, 1528, 1676, 1897
NlaIV	GGNNCC	6 cut(s)	1087, 1530, 1713, 1869, 1899, 1942
NmuCI	GTSAC	2 cut(s)	652, 760
NsbI	TGCGCA	1 cut(s)	856
NspI	RCATGY	2 cut(s)	466, 1676
NspV	TTCGAA	1 cut(s)	1793
OliI	CACNNNNGTG	1 cut(s)	1412
PaeI	GCATGC	1 cut(s)	466
PauI	GCGCGC	1 cut(s)	422
PctI	GAATGC	1 cut(s)	1071
PdmI	GAANNNNTTC	2 cut(s)	590, 1916
PfeI	GAWTC	2 cut(s)	1370, 1970
PflFI	GACNNNGTC	1 cut(s)	650
PinAI	ACCGGT	1 cut(s)	341
PkrI	GCNGC	5 cut(s)	489, 1107, 1507, 1846, 1981
PleI	GAGTC	1 cut(s)	825
PpsI	GAGTC	1 cut(s)	825
PsiI	TTATAA	1 cut(s)	1065
Psp124BI	GAGCTC	1 cut(s)	1727
Psp1406I	AACGTT	1 cut(s)	1904
PspN4I	GGNNCC	6 cut(s)	1087, 1530, 1713, 1869, 1899, 1942
PspPI	GGNCC	7 cut(s)	293, 401, 737, 1803, 1867, 1897, 1958
PstI	CTGCAG	1 cut(s)	1819
PsuI	RGATCY	2 cut(s)	678, 1711
PsyI	GACNNNGTC	1 cut(s)	650
PteI	GCGCGC	1 cut(s)	422
RsaI	GTAC	4 cut(s)	340, 449, 1057, 1244
RsaNI	GTAC	4 cut(s)	339, 448, 1056, 1243
RseI	CAYNNNNRTG	3 cut(s)	267, 758, 1412
SacI	GAGCTC	1 cut(s)	1727
SaqAI	TTAA	4 cut(s)	444, 794, 1101, 1434
SatI	GCNGC	5 cut(s)	488, 1106, 1506, 1845, 1980
Sau3AI	GATC	3 cut(s)	504, 678, 1711
Sau96I	GGNCC	7 cut(s)	293, 401, 737, 1803, 1867, 1897, 1958
ScaI	AGTACT	1 cut(s)	449
SchI	GAGTC	1 cut(s)	826
ScrFI	CCNGG	2 cut(s)	400, 1945
SduI	GDGCHC	2 cut(s)	287, 1727
SfaNI	GCATC	3 cut(s)	355, 842, 994
SfcI	CTRYAG	4 cut(s)	331, 828, 1576, 1815
SfuI	TTCGAA	1 cut(s)	1793
SinI	GGWCC	3 cut(s)	293, 1867, 1897
SmiMI	CAYNNNNRTG	3 cut(s)	267, 758, 1412
SmlI	CTYRAG	3 cut(s)	280, 318, 443
SmoI	CTYRAG	3 cut(s)	280, 318, 443
SphI	GCATGC	1 cut(s)	466
Sse9I	AATT	9 cut(s)	229, 370, 564, 846, 1139, 1587, 1693, 1730, 1783
SsiI	CCGC	1 cut(s)	487
SspI	AATATT	1 cut(s)	222
SspMI	CTAG	2 cut(s)	890, 923
SstI	GAGCTC	1 cut(s)	1727
StyD4I	CCNGG	2 cut(s)	398, 1943
StyI	CCWWGG	1 cut(s)	922
TaaI	ACNGT	3 cut(s)	658, 1060, 2054
TaiI	ACGT	2 cut(s)	1416, 1907
TaqI	TCGA	3 cut(s)	1793, 1880, 1964
TasI	AATT	9 cut(s)	229, 370, 564, 846, 1139, 1587, 1693, 1730, 1783
TatI	WGTACW	2 cut(s)	447, 1242
TauI	GCSGC	1 cut(s)	490
TfiI	GAWTC	2 cut(s)	1370, 1970
Tru1I	TTAA	4 cut(s)	444, 794, 1101, 1434
Tru9I	TTAA	4 cut(s)	444, 794, 1101, 1434
TscAI	CASTG	3 cut(s)	678, 1615, 1781
TseFI	GTSAC	2 cut(s)	652, 760
TseI	GCWGC	4 cut(s)	1105, 1505, 1844, 1979
Tsp45I	GTSAC	2 cut(s)	652, 760
TspDTI	ATGAA	4 cut(s)	285, 1715, 1755, 1962
TspGWI	ACGGA	2 cut(s)	134, 530
TspRI	CASTG	3 cut(s)	678, 1615, 1781
Tth111I	GACNNNGTC	1 cut(s)	650
Vha464I	CTTAAG	1 cut(s)	443
VpaK11BI	GGWCC	3 cut(s)	293, 1867, 1897
XagI	CCTNNNNNAGG	2 cut(s)	723, 2013
XapI	RAATTY	3 cut(s)	1139, 1693, 1730
XceI	RCATGY	2 cut(s)	466, 1676
XmaJI	CCTAGG	1 cut(s)	922
XmiI	GTMKAC	3 cut(s)	732, 1236, 1575
XmnI	GAANNNNTTC	2 cut(s)	590, 1916
XspI	CTAG	2 cut(s)	890, 923
ZrmI	AGTACT	1 cut(s)	449

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.