Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0002164

Physical Location & Seq

Genomic Coordinates Forward (+)

24421758 .. 24430558

Gene Structure 8801 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000033201

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3072 bp

ATGGGGTTCGTGATTTCGAACTCTAGGATTGGTTTGGTCTTTGCTTTTGGGTTTCTGGTGCTGATTTGCTTCACTGGGTTTGGGTCTCATGCCCAACTCATTCCTTTACAAGAAGTGAAAACTCTTGAAACGATATCCCAGAAACTCCAGAATTCTTTTTGGAACAACATCAGCCAAAGTTCTTGCCAAGATGGTGGATCAAGCTTTAATAAGAATATCACGGCCAATTTTCTAAGCAATGTCACTTGCGACTGTTCTTTCGCAAACAACACTTGCCATGTCACAAACATAGTGATGAAGGGTCTAAATTTAACTGGTATTCTACCTGAGGAATTTGGAAATCTTACTCATCTACAAGAACTTGATCTCACTCGTAACTACATCAATGGATCAATTCCTGCAAACATTTCTCGTGCCCCTCTTCAGGTTCTGTCTCTTTTAGGAAATCGACTAAGTGGTTCTATTCCAAAGGAAATTGGTGACATTGCTACTCTTACTCAGCTTGTGTTGGAAGATAATCAACTTGAAGGGCCTCTTCCTCAAAACCTTGGGAGTTTGAGTTCCTTGGAGCGACTCCTTCTTTCTGCGAACAACTTTACTGGAATAATTCCAGAATCATTTGGAAATCTAAAGAATTTAACCGATTTTAGGATAGATGGGAGCCGAATCTCAGGGAAAATACCTACTTTTATTGGAAACTGGACCAAACTTGACAGACTGGATATGCAAGGCACGTCCATGGAAGGCCCCATTCCTTCCACCATATCTTTGCTGACAAACTTAACTGAATTGAGAATATCCGATTTGAATGGATCAAGTATGCCTTTTCCTAATTTGGAGGATTTGAAAATTTTGGAAAGAGTGATTCTGAGAAATTGCTCAATTACCAATTCTATCCCTGGCTACATTGGAGAGCTGACAAAATTAAAGGAATTGGACCTGACCTTTAACAAGTTGACAGGTGAAGTTCCAACGACCCTTGAGAATCTTGTTGCTCTTGATTACATGTTTTTGACCAACAATTTACTGACTGGAGAAGTACCAAGCTGGATATTGAGCAACAAAAATAACTTTGATTTGTCTTACAACAATTTTAGCGGATCACTTACAATTAGTTGTCAACAGTTACCAGTGAATTTAATTTCTAGCTTTTCATCTTCACAAAGCTCGTGGTGCTTGCAGAAGGACCTCCCTTGTTCCACAAAAGCCAAACACTATTCTTTGTTTATTAATTGTGGGGGGAGACTGATGGAATTCAATGGTAATCAGTACGAAGAGGACTTGAGCACTGTGGGATCGTCAGACTTCATTTCCTCATCTGAGAAGTGGGGTTATAGCAGTACAGGTGTTTACATGGGGAATGATGATGCAGATTATACAGCAACAAACACATTGCCTCTTAATGTTAATGGTCCAGAATTTTATCAAACTGCCCGCCTTTCCCCACTCTCACTCAAGTATTATGGCCTCTGCATGCTTAAGGGTAGTTACAAAGTGCAGCTCCACTTTGCTGAAATCATGTATTCAGATAATGACACATTCACTGGCCTCGGGAAGCGCATATTTGATGTGTCAATTCAAGGAAATTTGGTTTTAAAGGATTTCAATATTATGAAGGAAGCAGGAGGTGCTGGTAAAGACATCGTCAAAGAATTTAATGTCCTTGTTAATGGTAGCACTCTAGAAATCCACTTATACTGGAAAGGAAAAGGAACTACTGCAATACCTGATAGGGGTGTCTATGGACCTCTTATATCTGCAATTACTGTGACACCAAATTTTAAGGTTGATATTGGTGGTGGGGGCCTATCTGCTGGAGCTATTGCTGGCATTGTAGTAGCATCATGTGTGTTGATTGTATTGATTTTGGCAGTCCTTCGATATATGGGTTACTTGGGGGGCAAAGACCTTGAAGATAAGGCCAAACTTCCAATACACATAGCCAACCTAGTAAATTACATATCTGTAGGGTTGATAATAAGAAGTTTCGAGCCTAGGACCCAAATTAAAGCTTTGATACCATGGTTAATCAGAAGACCCAAACTGGGGGTACTACCAGATGGTGCTGTAATTGCTGTTAAGCAGCTCTCATCTAAATCAAAGCAAGGGAACCGTGAATTTGTTAATGAGATAGGCATGATATCTGCTTTGCAACACCCAAATCTTGTGAAGCTTTTCGGTTGCTGTATCGAAGGAAACCAGTTATTGCTTATATATGAATACATGGAAAACAATAGTCTTGCCCGCGCACTTTTTGGTCGTGAGGAACAACGGCTACACTTGGACTGGAAAACAAGAAAGAAGATATGCATGGGGATAGCGAGAGGGTTGGCCTATCTTCATGAAGAATCAAGGTTGAAAATTGTTCACAGGGATATAAAGGCAACTAATGTCCTGCTTGATAAGGATCTGAATGCCAAAATATCTGATTTTGGTTTAGCTAAGCTGGATGAAGAAGAGAACACACATATCAGCACTCGAATAGCTGGAACAATAGGCTATATGGCACCTGAATACGCAATGAGGGGATACTTGACTGACAAAGCAGATGTGTATAGTTTTGGTATTGTTGCCTTAGAAATAGTCAGTGGAAAGAGCAACACAAATTACAGACCAAAGGAGGAGTACGTGTATCTCCTTGACGGGGCCTATGTCCTACAAGAGCAAGGAAATATGTTAGAACTTGTGGATCCAGATCTTGGTTCAAACTACTCCAAAGAAGAGGCGATGACAATGCTTAACGTGGCGCTTTTATGCACCAATCCATCTCCCACTCTCAGACCACCCATGTCATCTGTCATAAGCATGCTTGAAGGTAAAATTCCGATTCAAGCACAGTTAATCAAGCGCGACACGATGGAGCAGGATGCGAGGTTCAAAGCCTTTGAGAGACTATCACAAGACAGCAAAACACATGTTTCAACTTTCTCGCAAGATAGTCATGTAAGAGGAACATCAATGGAAGGTCCATGGATTGATTCTTCCGTTTCTCTCGCTAGTAAAAATGAGTCCCATGAACATTCTTCAACAAGCACGCAACTCAAGGATCTGTATGATGTAAACTTACAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_1 LRR_8 Malectin Pkinase Pkinase_Tyr

Protein Analysis

1024

Amino Acids

112.83

Weight (kDa)

5.79

Isoelectric Point (pI)

33.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	207 - 323	4.7e-09	Leucine-rich repeat region
LRR_8	PF13855	285 - 343	1.4e-06	Leucine rich repeat
Malectin	PF11721	407 - 588	7.7e-39	Malectin domain
Pkinase	PF00069	687 - 922	5.7e-41	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	690 - 935	1.3e-43	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0002164)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53430 AT1G53430 AT1G53440
fragaria_vesca	FvH4_3g17940
malus_domestica	MD03G1247100.v1.1 MD03G1248200.v1.1 MD03G1248300.v1.1 MD03G1249100.v1.1 MD03G1249200.v1.1 MD11G1267500.v1.1
prunus_persica	Prupe.4G158000_v2.0.a1 Prupe.4G158000_v2.0.a1
pyrus_communis	pycom03g19620 pycom03g19750 pycom06g03840 pycom11g23530
rosa_chinensis	RchiOBHm_Chr5g0029911 RchiOBHm_Chr5g0029981
rosa_laevigata	RLG00000033201 RLG00000033206
rosa_multiflora	Rmu_sc0003368.1_g000014 Rmu_sc0004542.1_g000020 Rmu_sc0021047.1_g000001
rosa_roxburghii	Rroxscaffold_1G00050050
rosa_rugosa	Rorug05G0115300 Rorug05G0115400 Rorug05G0115500 Rorug05G0115600 Rorug05G0115700 Rorug05G0115800 Rorug05G0115900 Rorug05G0116000 Rorug05G0116100 Rorug05G0116200
rosa_samantha	Rh5AG208900 Rh5AG209100 Rh5BG207700 Rh5CG231100 Rh5CG231800 Rh5DG211900 Rh5DG212500
rosa_wichuraiana	Rw5G019010 Rw5G019050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	2506
AccB7I	CCANNNNNTGG	1 cut(s)	2699
AccII	CGCG	2 cut(s)	2247, 2850
AciI	CCGC	3 cut(s)	1098, 1435, 2245
AclWI	GGATC	9 cut(s)	205, 397, 820, 1108, 1303, 2415, 2684, 2697, 3054
AcoI	YGGCCR	1 cut(s)	222
AcuI	CTGAAG	1 cut(s)	407
AfaI	GTAC	5 cut(s)	1041, 1271, 1342, 2052, 2627
AfiI	CCNNNNNNNGG	7 cut(s)	424, 648, 1733, 2045, 2046, 2644, 2699
AflII	CTTAAG	1 cut(s)	1478
AflIII	ACRYGT	3 cut(s)	1005, 2628, 2914
AjiI	CACGTC	1 cut(s)	735
AjnI	CCWGG	1 cut(s)	898
AloI	GAACNNNNNNTCC	4 cut(s)	544, 576, 2858, 2890
Alw21I	GWGCWC	1 cut(s)	1289
Alw26I	GTCTC	4 cut(s)	90, 438, 1237, 2884
AlwI	GGATC	9 cut(s)	205, 397, 820, 1108, 1303, 2415, 2684, 2697, 3054
AlwNI	CAGNNNCTG	1 cut(s)	430
Ama87I	CYCGRG	1 cut(s)	1550
AoxI	GGCC	9 cut(s)	222, 530, 745, 1465, 1546, 1804, 1920, 2331, 2646
ApeKI	GCWGC	2 cut(s)	1498, 2083
ArsI	GACNNNNNNTTYG	2 cut(s)	242, 274
AseI	ATTAAT	1 cut(s)	1230
Asp700I	GAANNNNTTC	1 cut(s)	2174
AspA2I	CCTAGG	1 cut(s)	1994
AspLEI	GCGC	4 cut(s)	1560, 2249, 2749, 2850
AsuHPI	GGTGA	2 cut(s)	491, 974
AsuII	TTCGAA	1 cut(s)	17
AvaI	CYCGRG	1 cut(s)	1550
AvaII	GGWCC	7 cut(s)	702, 937, 1186, 1412, 1745, 1998, 2966
AvrII	CCTAGG	1 cut(s)	1994
AxyI	CCTNAGG	1 cut(s)	327
BaeGI	GKGCMC	1 cut(s)	418
BaeI	ACNNNNGTAYC	2 cut(s)	2009, 2042
BamHI	GGATCC	1 cut(s)	2689
BanI	GGYRCC	1 cut(s)	2506
BauI	CACGAG	2 cut(s)	411, 1168
BbsI	GAAGAC	1 cut(s)	2041
Bbv12I	GWGCWC	1 cut(s)	1289
BbvI	GCAGC	2 cut(s)	1510, 2095
BccI	CCATC	6 cut(s)	185, 650, 1243, 2054, 2773, 2851
BceAI	ACGGC	2 cut(s)	237, 2288
BcgI	CGANNNNNNTGC	2 cut(s)	2716, 2750
BciT130I	CCWGG	1 cut(s)	900
BciVI	GTATCC	1 cut(s)	2522
BcoDI	GTCTC	4 cut(s)	90, 438, 1237, 2884
BfaI	CTAG	6 cut(s)	24, 1146, 1682, 1949, 1995, 2997
BfmI	CTRYAG	1 cut(s)	1965
BfoI	RGCGCY	1 cut(s)	2750
BfrI	CTTAAG	1 cut(s)	1478
BfuI	GTATCC	1 cut(s)	2522
BglII	AGATCT	1 cut(s)	2695
BisI	GCNGC	2 cut(s)	1499, 2084
BlnI	CCTAGG	1 cut(s)	1994
BlpI	GCTNAGC	1 cut(s)	2442
BlsI	GCNGC	2 cut(s)	1500, 2085
Bme1390I	CCNGG	1 cut(s)	900
Bme18I	GGWCC	7 cut(s)	702, 937, 1186, 1412, 1745, 1998, 2966
BmeT110I	CYCGRG	1 cut(s)	1550
BmgBI	CACGTC	1 cut(s)	735
BmiI	GGNNCC	8 cut(s)	662, 748, 1805, 2000, 2111, 2508, 2647, 2691
BmrFI	CCNGG	1 cut(s)	900
BmrI	ACTGGG	2 cut(s)	84, 2054
BmsI	GCATC	3 cut(s)	1357, 1850, 2857
BmuI	ACTGGG	2 cut(s)	84, 2054
BpiI	GAAGAC	1 cut(s)	2041
BpmI	CTGGAG	3 cut(s)	131, 1053, 1836
Bpu1102I	GCTNAGC	1 cut(s)	2442
Bpu14I	TTCGAA	1 cut(s)	17
BpuEI	CTTGAG	4 cut(s)	1001, 1303, 1439, 3026
BsaAI	YACGTR	1 cut(s)	2629
BsaBI	GATNNNNATC	2 cut(s)	2406, 2694
BsaI	GGTCTC	1 cut(s)	90
BsaJI	CCNNGG	8 cut(s)	547, 564, 738, 898, 1549, 1994, 2021, 2969
BsaXI	ACNNNNNCTCC	4 cut(s)	544, 574, 2615, 2645
Bsc4I	CCNNNNNNNGG	7 cut(s)	424, 648, 1733, 2045, 2046, 2644, 2699
Bse21I	CCTNAGG	1 cut(s)	327
Bse3DI	GCAATG	4 cut(s)	244, 483, 1391, 2526
Bse8I	GATNNNNATC	2 cut(s)	2406, 2694
BseBI	CCWGG	1 cut(s)	900
BseDI	CCNNGG	8 cut(s)	547, 564, 738, 898, 1549, 1994, 2021, 2969
BseGI	GGATG	2 cut(s)	2455, 2872
BseJI	GATNNNNATC	2 cut(s)	2406, 2694
BseLI	CCNNNNNNNGG	7 cut(s)	424, 648, 1733, 2045, 2046, 2644, 2699
BseMI	GCAATG	4 cut(s)	244, 483, 1391, 2526
BseMII	CTCAG	6 cut(s)	318, 512, 684, 860, 1311, 2791
BseRI	GAGGAG	1 cut(s)	2636
BseSI	GKGCMC	1 cut(s)	418
BseXI	GCAGC	2 cut(s)	1510, 2095
BsgI	GTGCAG	1 cut(s)	1517
Bsh1236I	CGCG	2 cut(s)	2247, 2850
BshFI	GGCC	9 cut(s)	224, 532, 747, 1467, 1548, 1806, 1922, 2333, 2648
BshNI	GGYRCC	1 cut(s)	2506
BsiHKAI	GWGCWC	1 cut(s)	1289
BsiHKCI	CYCGRG	1 cut(s)	1550
BslFI	GGGAC	1 cut(s)	2995
BslI	CCNNNNNNNGG	7 cut(s)	424, 648, 1733, 2045, 2046, 2644, 2699
BsmAI	GTCTC	4 cut(s)	90, 438, 1237, 2884
BsmFI	GGGAC	1 cut(s)	2995
BsmI	GAATGC	1 cut(s)	2419
BsnI	GGCC	9 cut(s)	224, 532, 747, 1467, 1548, 1806, 1922, 2333, 2648
Bso31I	GGTCTC	1 cut(s)	90
BsoBI	CYCGRG	1 cut(s)	1550
Bsp119I	TTCGAA	1 cut(s)	17
Bsp1286I	GDGCHC	2 cut(s)	418, 1289
Bsp1720I	GCTNAGC	1 cut(s)	2442
Bsp19I	CCATGG	3 cut(s)	738, 2021, 2969
BspACI	CCGC	3 cut(s)	1098, 1435, 2245
BspANI	GGCC	9 cut(s)	224, 532, 747, 1467, 1548, 1806, 1922, 2333, 2648
BspCNI	CTCAG	6 cut(s)	319, 511, 683, 861, 1312, 2790
BspFNI	CGCG	2 cut(s)	2247, 2850
BspHI	TCATGA	1 cut(s)	2341
BspLI	GGNNCC	8 cut(s)	662, 748, 1805, 2000, 2111, 2508, 2647, 2691
BspPI	GGATC	9 cut(s)	205, 397, 820, 1108, 1303, 2415, 2684, 2697, 3054
BspT104I	TTCGAA	1 cut(s)	17
BspT107I	GGYRCC	1 cut(s)	2506
BspTI	CTTAAG	1 cut(s)	1478
BspTNI	GGTCTC	1 cut(s)	90
BsrDI	GCAATG	4 cut(s)	244, 483, 1391, 2526
BssECI	CCNNGG	8 cut(s)	547, 564, 738, 898, 1549, 1994, 2021, 2969
BssSI	CACGAG	2 cut(s)	411, 1168
BssT1I	CCWWGG	6 cut(s)	547, 564, 738, 1994, 2021, 2969
Bst2BI	CACGAG	2 cut(s)	411, 1168
Bst2UI	CCWGG	1 cut(s)	900
Bst4CI	ACNGT	7 cut(s)	254, 1125, 1291, 1768, 2114, 2838, 3069
Bst6I	CTCTTC	5 cut(s)	426, 540, 1269, 2451, 2715
BstAFI	CTTAAG	1 cut(s)	1478
BstAPI	GCANNNNNTGC	1 cut(s)	1628
BstBAI	YACGTR	1 cut(s)	2629
BstBI	TTCGAA	1 cut(s)	17
BstC8I	GCNNGC	7 cut(s)	1178, 1435, 1475, 1828, 2245, 2807, 3035
BstDSI	CCRYGG	3 cut(s)	738, 2021, 2969
BstF5I	GGATG	2 cut(s)	2455, 2872
BstFNI	CGCG	2 cut(s)	2247, 2850
BstH2I	RGCGCY	1 cut(s)	2750
BstHHI	GCGC	4 cut(s)	1560, 2249, 2749, 2850
BstMAI	GTCTC	4 cut(s)	90, 438, 1237, 2884
BstMWI	GCNNNNNNNGC	3 cut(s)	1173, 1628, 2072
BstNI	CCWGG	1 cut(s)	900
BstNSI	RCATGY	4 cut(s)	1009, 1477, 2809, 2918
BstSCI	CCNGG	1 cut(s)	898
BstSFI	CTRYAG	1 cut(s)	1965
BstSLI	GKGCMC	1 cut(s)	418
BstUI	CGCG	2 cut(s)	2247, 2850
BstV1I	GCAGC	2 cut(s)	1510, 2095
BstV2I	GAAGAC	1 cut(s)	2041
BstX2I	RGATCY	4 cut(s)	2407, 2689, 2695, 3046
BstXI	CCANNNNNNTGG	1 cut(s)	194
BstYI	RGATCY	4 cut(s)	2407, 2689, 2695, 3046
Bsu36I	CCTNAGG	1 cut(s)	327
BsuI	GTATCC	1 cut(s)	2522
BsuRI	GGCC	9 cut(s)	224, 532, 747, 1467, 1548, 1806, 1922, 2333, 2648
BtgI	CCRYGG	3 cut(s)	738, 2021, 2969
BtgZI	GCGATG	1 cut(s)	2741
BtrI	CACGTC	1 cut(s)	735
BtsCI	GGATG	2 cut(s)	2455, 2872
BtsIMutI	CAGTG	5 cut(s)	72, 1137, 1287, 1542, 2593
Cac8I	GCNNGC	7 cut(s)	1178, 1435, 1475, 1828, 2245, 2807, 3035
CaiI	CAGNNNCTG	1 cut(s)	430
CciI	TCATGA	1 cut(s)	2341
CfoI	GCGC	4 cut(s)	1560, 2249, 2749, 2850
Csp6I	GTAC	5 cut(s)	1040, 1270, 1341, 2051, 2626
CspCI	CAANNNNNGTGG	2 cut(s)	1151, 1186
CviQI	GTAC	5 cut(s)	1040, 1270, 1341, 2051, 2626
DraI	TTTAAA	1 cut(s)	1596
EaeI	YGGCCR	1 cut(s)	222
Eam1104I	CTCTTC	5 cut(s)	426, 540, 1269, 2451, 2715
EarI	CTCTTC	5 cut(s)	426, 540, 1269, 2451, 2715
Eco130I	CCWWGG	6 cut(s)	547, 564, 738, 1994, 2021, 2969
Eco31I	GGTCTC	1 cut(s)	90
Eco32I	GATATC	2 cut(s)	135, 2142
Eco47I	GGWCC	7 cut(s)	702, 937, 1186, 1412, 1745, 1998, 2966
Eco57I	CTGAAG	1 cut(s)	407
Eco81I	CCTNAGG	1 cut(s)	327
Eco88I	CYCGRG	1 cut(s)	1550
EcoO109I	RGGNCCY	6 cut(s)	530, 746, 1186, 1804, 1998, 2646
EcoRI	GAATTC	2 cut(s)	151, 1253
EcoRII	CCWGG	1 cut(s)	898
EcoRV	GATATC	2 cut(s)	135, 2142
EcoT14I	CCWWGG	6 cut(s)	547, 564, 738, 1994, 2021, 2969
EcoT22I	ATGCAT	1 cut(s)	2312
ErhI	CCWWGG	6 cut(s)	547, 564, 738, 1994, 2021, 2969
FalI	AAGNNNNNCTT	4 cut(s)	519, 551, 808, 840
FaqI	GGGAC	1 cut(s)	2995
FauI	CCCGC	2 cut(s)	1442, 2252
Fnu4HI	GCNGC	2 cut(s)	1499, 2084
FokI	GGATG	2 cut(s)	2462, 2879
Fsp4HI	GCNGC	2 cut(s)	1499, 2084
FspBI	CTAG	6 cut(s)	24, 1146, 1682, 1949, 1995, 2997
GlaI	GCGC	4 cut(s)	1559, 2248, 2748, 2849
GluI	GCNGC	2 cut(s)	1499, 2084
GsuI	CTGGAG	3 cut(s)	131, 1053, 1836
HaeII	RGCGCY	1 cut(s)	2750
HaeIII	GGCC	9 cut(s)	224, 532, 747, 1467, 1548, 1806, 1922, 2333, 2648
HhaI	GCGC	4 cut(s)	1560, 2249, 2749, 2850
Hin6I	GCGC	4 cut(s)	1558, 2247, 2747, 2848
HinP1I	GCGC	4 cut(s)	1558, 2247, 2747, 2848
HincII	GTYRAC	2 cut(s)	957, 1121
HindII	GTYRAC	2 cut(s)	957, 1121
HindIII	AAGCTT	3 cut(s)	202, 2010, 2171
HinfI	GANTC	9 cut(s)	573, 614, 666, 865, 985, 2348, 2827, 2978, 3008
HphI	GGTGA	2 cut(s)	491, 974
Hpy166II	GTNNAC	5 cut(s)	957, 1121, 1351, 2368, 3061
Hpy8I	GTNNAC	5 cut(s)	957, 1121, 1351, 2368, 3061
HpyCH4III	ACNGT	7 cut(s)	254, 1125, 1291, 1768, 2114, 2838, 3069
HpyCH4IV	ACGT	3 cut(s)	734, 2628, 2742
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1173, 1628, 2072
HpySE526I	ACGT	3 cut(s)	734, 2628, 2742
HspAI	GCGC	4 cut(s)	1558, 2247, 2747, 2848
LmnI	GCTCC	5 cut(s)	568, 660, 1506, 1817, 2860
Lsp1109I	GCAGC	2 cut(s)	1510, 2095
LweI	GCATC	3 cut(s)	1357, 1850, 2857
MaeI	CTAG	6 cut(s)	24, 1146, 1682, 1949, 1995, 2997
MaeII	ACGT	3 cut(s)	734, 2628, 2742
MaeIII	GTNAC	8 cut(s)	241, 280, 374, 479, 1125, 1487, 1768, 1889
MflI	RGATCY	4 cut(s)	2407, 2689, 2695, 3046
MhlI	GDGCHC	2 cut(s)	418, 1289
MlyI	GAGTC	2 cut(s)	567, 3017
MmeI	TCCRAC	2 cut(s)	489, 995
Mph1103I	ATGCAT	1 cut(s)	2312
MroXI	GAANNNNTTC	1 cut(s)	2174
MslI	CAYNNNNRTG	3 cut(s)	293, 737, 2804
MspCI	CTTAAG	1 cut(s)	1478
MspR9I	CCNGG	1 cut(s)	900
Mva1269I	GAATGC	1 cut(s)	2419
MvaI	CCWGG	1 cut(s)	900
MvnI	CGCG	2 cut(s)	2247, 2850
MwoI	GCNNNNNNNGC	3 cut(s)	1173, 1628, 2072
NcoI	CCATGG	3 cut(s)	738, 2021, 2969
NlaIV	GGNNCC	8 cut(s)	662, 748, 1805, 2000, 2111, 2508, 2647, 2691
NmuCI	GTSAC	4 cut(s)	241, 280, 479, 1768
NsiI	ATGCAT	1 cut(s)	2312
NspI	RCATGY	4 cut(s)	1009, 1477, 2809, 2918
NspV	TTCGAA	1 cut(s)	17
PaeI	GCATGC	2 cut(s)	1477, 2809
PagI	TCATGA	1 cut(s)	2341
PciI	ACATGT	2 cut(s)	1005, 2914
PctI	GAATGC	1 cut(s)	2419
PdmI	GAANNNNTTC	1 cut(s)	2174
PfeI	GAWTC	7 cut(s)	614, 666, 865, 985, 2348, 2827, 2978
PflFI	GACNNNGTC	1 cut(s)	1643
PflMI	CCANNNNNTGG	1 cut(s)	2699
PkrI	GCNGC	2 cut(s)	1500, 2085
PleI	GAGTC	2 cut(s)	567, 3016
PpsI	GAGTC	2 cut(s)	567, 3016
Ppu21I	YACGTR	1 cut(s)	2629
PpuMI	RGGWCCY	2 cut(s)	1186, 1998
PscI	ACATGT	2 cut(s)	1005, 2914
PshBI	ATTAAT	1 cut(s)	1230
Psp5II	RGGWCCY	2 cut(s)	1186, 1998
Psp6I	CCWGG	1 cut(s)	898
PspGI	CCWGG	1 cut(s)	898
PspN4I	GGNNCC	8 cut(s)	662, 748, 1805, 2000, 2111, 2508, 2647, 2691
PspPPI	RGGWCCY	2 cut(s)	1186, 1998
PsrI	GAACNNNNNNTAC	2 cut(s)	2259, 2291
PstNI	CAGNNNCTG	1 cut(s)	430
PsuI	RGATCY	4 cut(s)	2407, 2689, 2695, 3046
PsyI	GACNNNGTC	1 cut(s)	1643
RsaI	GTAC	5 cut(s)	1041, 1271, 1342, 2052, 2627
RsaNI	GTAC	5 cut(s)	1040, 1270, 1341, 2051, 2626
RseI	CAYNNNNRTG	3 cut(s)	293, 737, 2804
SatI	GCNGC	2 cut(s)	1499, 2084
SchI	GAGTC	2 cut(s)	567, 3017
ScrFI	CCNGG	1 cut(s)	900
SduI	GDGCHC	2 cut(s)	418, 1289
SfaNI	GCATC	3 cut(s)	1357, 1850, 2857
SfcI	CTRYAG	1 cut(s)	1965
SfuI	TTCGAA	1 cut(s)	17
SinI	GGWCC	7 cut(s)	702, 937, 1186, 1412, 1745, 1998, 2966
SmiMI	CAYNNNNRTG	3 cut(s)	293, 737, 2804
SmlI	CTYRAG	5 cut(s)	980, 1282, 1454, 1478, 3041
SmoI	CTYRAG	5 cut(s)	980, 1282, 1454, 1478, 3041
SphI	GCATGC	2 cut(s)	1477, 2809
SsiI	CCGC	3 cut(s)	1098, 1435, 2245
SspI	AATATT	1 cut(s)	1609
SspMI	CTAG	6 cut(s)	24, 1146, 1682, 1949, 1995, 2997
StyD4I	CCNGG	1 cut(s)	898
StyI	CCWWGG	6 cut(s)	547, 564, 738, 1994, 2021, 2969
TaaI	ACNGT	7 cut(s)	254, 1125, 1291, 1768, 2114, 2838, 3069
TaiI	ACGT	3 cut(s)	737, 2631, 2745
TaqI	TCGA	6 cut(s)	17, 448, 1879, 1989, 2190, 2479
TatI	WGTACW	1 cut(s)	1340
TfiI	GAWTC	7 cut(s)	614, 666, 865, 985, 2348, 2827, 2978
TscAI	CASTG	5 cut(s)	79, 1137, 1294, 1549, 2593
TseFI	GTSAC	4 cut(s)	241, 280, 479, 1768
TseI	GCWGC	2 cut(s)	1498, 2083
Tsp45I	GTSAC	4 cut(s)	241, 280, 479, 1768
TspDTI	ATGAA	9 cut(s)	311, 1143, 1297, 1628, 2232, 2330, 2358, 2466, 3030
TspGWI	ACGGA	1 cut(s)	2974
TspRI	CASTG	5 cut(s)	79, 1137, 1294, 1549, 2593
Tth111I	GACNNNGTC	1 cut(s)	1643
Van91I	CCANNNNNTGG	1 cut(s)	2699
Vha464I	CTTAAG	1 cut(s)	1478
VpaK11BI	GGWCC	7 cut(s)	702, 937, 1186, 1412, 1745, 1998, 2966
VspI	ATTAAT	1 cut(s)	1230
XbaI	TCTAGA	1 cut(s)	1681
XceI	RCATGY	4 cut(s)	1009, 1477, 2809, 2918
XmaJI	CCTAGG	1 cut(s)	1994
XmnI	GAANNNNTTC	1 cut(s)	2174
XspI	CTAG	6 cut(s)	24, 1146, 1682, 1949, 1995, 2997
Zsp2I	ATGCAT	1 cut(s)	2312

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.