RchiOBHm_Chr5g0029981

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002164
Physical Location & Seq
Forward (+)
23786326 .. 23799491
13166 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30934

Sequence Viewer

Length: 3027 bp
ATGGGGTTCGTGATTTCGAACTCTAGGATTGGTTTGGTCTTTGCTTTTGGGTTTCTGGTGCTGATTTGCTTCACTGGGTTTGGGTCTCATGCTCAACTCATTCCTCTACAAGAAGTGAAAACTCTTGAAACGATATCCCAGAAACTCCAGAATTCTTTTTGGAACATTAGCCAAAGTTCTTGCCAAGATGGTGGATCAAGCTTTAATAAGAATATCACGGACAAGATTCTAAGCAATGTCACTTGCGACTGTTCCTTCGCAAACAACACTTGCCATGTCACAAACATAGTGATGTACGGTCTAAATTTAACTGGTATTCTACCTGAGGAATTTGGAGATCTTACATATCTACAAGAACTTGATCTCACTCTTAACTACATCAATGGATCCATTCCTACAGGCATTTCTCGCGCCCCTCTTAGGAATCTGTCTCTTACAGGAAATCGACTAAGTGGTTCTATTCCAAAGGAAATTGGTGACATTGCTACTCTTACTGAGCTTTTGTTGGAAGATAATCAACTTGAAGGGCCTCTTCCTCAAAACCTTGGGAGTTTGAGTTCCTTGAATAGACTCCTTCTTTCTGCGAATAACTTTACAGGAACAATTCCAGAATCATTTGGAAATCTAAAGAACTTAACCGATTTTAGGATAGATGGGAGCCGAATCTCAGGAAAAATACCTACTTTTATTGGAAACTGGACCAAGCTTGACAGACTGGATATGCAAGGCACGTCCATGGAAGGCCCCATTCCTTCCACCATATCTTTGCTGACAAAGTTAACTCAATTGATCATATCTGATATGAATGGATCAAGTATGCATTTTCCTAAATTGGAGGATTTGAAAATTTTGAAAACATTGATTCTGAGAAATTGCTCAATTACCGATTCTATCCCTGACTACATTGGAGATATGACAAATTTAAAGATATTGGACCTGAGCTTTAACAAGTTGACAGGTGAAGTTCCAACGACCCTTGAGAATCTTGTTGCTCTTGATTACATGTTTTTGACCAACAATTTACTGACTGGAGAAGTACCAAGCTGGATATTGAGCAACAAAAATAACTTTGATTTGTCTTACAACAATTTTAGCGGATCACCTACAGTTGGTTGTCAACACTTATCAGTGAATTTAATTTCTAGCTTTTCATCTTCACAAAGCCCGTGGTGCTTGCAGAAGGACCTCCCTTGTTCCACAAAAGCCAAATACTATTCTGTGTTTATTAATTGTGGGGGGGGTCGGATGAAATTCAATGGTAATCAGTACGAAGAGGACTTGAGCCCTGAGGGACCGTCAGACTTCATTTCCTCATTTGAGAAGTGGGGTTATAGCAGTACAGGGATTTACATGGGGAAGGAAAAAGCAGATTACACAGCAACAAACACATCACCTCTTAATGGTTCACCAATTTATCAAACTGCCCGCCTTTCCCCGCTCTCACTCAAGTATTATGGCCTTTGCATGCTTAAGGGTAGTTACAAAGTGCAGCTCCACTTCGCTGAAATAAAGTATTCAGATAATGGCACATTCACTGACCTCGGGCAGCGTATATTTGATGTGTCAATTCAAGGAAATTTGGTTTTAAAGGATTTCAATATTATGAAGGAAGCAGGAGGTGCTGGTAAAGGCATCGTCAGAGAATTTAATGTCCTTGTTAATGGTAGCACTCTAGAAATCCACTTATCCTGGAAAGGAAAAGGAACTAATGCAATACCTGATAGGGGTGGCTATGGACCTCTTATATCTGCAATTACTGTGACACCAAATTTTAAGGTTGATATTGGCCTATCTGCTGGAGCTATTGCTGGCATTGTAGTAGCATCATGTGTGTTGATTGTATTGATTTTGGCAGTCCTTCGATATATGGGTTACTTGGGGGGCGAAGACCTTGAAGATAAAGAACTGCGTGGGCTAGAACTACAAACAGGTTATTTCACCTTACGACAAATTAAAGCTGCCACAGGTAACTTTGATCCTTCAAATAAGATAGGTGAAGGAGGTTTTGGGCCTGTTTACAAGGGGGTACTACTAGATGGTGCTGTAATTGCTGTTAAGCAGCTCTCATCTAAATCAAAGCAAGGGAACCGTGAATTTGTTAACGAGATAGGCATGATATCTGCTTTGCAACACCCAAATCTCGTGAAGCTTTTTGGTTGCTGTATCGAAGGAAACCAGTTACTGCTTATATATGAATACATGGAAAACAATAGTCTTGCCCGTGCACTTTTTGGTCGTGAAGAACAACGGCTACACTTGGACTGGAAAACAAGAAAGAAGATATGCATGGGGATAGCGAGAGGGTTGACCTATCTTCATGAAGAATCAAGGTTGAAAATTGTTCACAGGGATATAAAGGCAACTAATGTCCTGCTTGATAAGGATCTGAATGCCAAAATATCTGATTTTGGTTTAGCTAAGCTGGATGAGGAAGAGAACTCACATATCAGCACTCGAATAGCTGGAACAATAGGCTATATGGCACCTGAATACGCAATGAGGGGATACTTGACTGACAAAGCAGATGTGTACAGTTTTGGTATTGTTGCCTTAGAAATAGTCAGTGGAAAGAGCAACACAAATTACAGACCAAAGGAGGAGTACGTGTATCTCCTTGATTGGGCCTATGTCCTACAAGAGCAAGGAAATATGTTAGAACTTGTGGATCCAGATCTTGGTTCAAACTACTCCAAAGAACAGGCGATGACAATGCTTAACTTGGCGCTCTTATGCACCAATCCATCTCCCACTCTCAGACCACCCATGTCATCTGTCGTAAGCATGCTTGAAGGTAAAAGTCCGATTCAAGCACCGTTAATCAAGGGCGACACGATGGAGCAGGATGCGAGGTTCAAAGCCTTTGAGAGACTATCACAAGACAGCCAAACACAAGTTTCAACTCTCTCGCAAGATAGTCTTGTAAGAGGAACATCAATGGAAGGTCCATTGATTGATTCTTCGGTTTCTCTCGCTAGTAAGGATGAGTCCCATGAACATCCTTCAACAAGCCTACTAAAGGATCTGTAG

Protein Analysis

1008

Amino Acids

110.74

Weight (kDa)

5.49

Isoelectric Point (pI)

31.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 224 - 322 1e-07 Leucine-rich repeat region
LRR_8 PF13855 284 - 342 2.3e-08 Leucine rich repeat
LRR_4 PF12799 284 - 319 6.8e-06 Leucine Rich repeats (2 copies)
Malectin PF11721 407 - 585 2.8e-37 Malectin domain
Pkinase PF00069 661 - 920 9.3e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 662 - 929 1.2e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2482
AccB7I CCANNNNNTGG 1 cut(s) 2675
AccBSI CCGCTC 1 cut(s) 1438
AccII CGCG 1 cut(s) 411
AciI CCGC 3 cut(s) 1095, 1426, 1436
AclWI GGATC 9 cut(s) 202, 381, 394, 817, 1105, 1970, 2391, 2660, 2673
AfaI GTAC 7 cut(s) 296, 1038, 1268, 1339, 2028, 2531, 2603
AfiI CCNNNNNNNGG 8 cut(s) 420, 645, 1109, 1400, 1724, 2620, 2675, 3016
AflII CTTAAG 1 cut(s) 1469
AflIII ACRYGT 2 cut(s) 1002, 2604
AjiI CACGTC 1 cut(s) 732
AjnI CCWGG 1 cut(s) 1688
AjuI GAANNNNNNNTTGG 2 cut(s) 1989, 2021
AloI GAACNNNNNNTCC 4 cut(s) 541, 573, 2834, 2866
Alw21I GWGCWC 1 cut(s) 2227
Alw26I GTCTC 3 cut(s) 90, 435, 2860
Alw44I GTGCAC 1 cut(s) 2223
AlwI GGATC 9 cut(s) 202, 381, 394, 817, 1105, 1970, 2391, 2660, 2673
AlwNI CAGNNNCTG 1 cut(s) 2182
Ama87I CYCGRG 1 cut(s) 1541
AoxI GGCC 6 cut(s) 527, 742, 1456, 1786, 2009, 2622
ApaLI GTGCAC 1 cut(s) 2223
ApeKI GCWGC 4 cut(s) 1489, 1546, 1958, 2059
ArsI GACNNNNNNTTYG 2 cut(s) 239, 271
AseI ATTAAT 1 cut(s) 1227
AspLEI GCGC 2 cut(s) 413, 2725
AsuHPI GGTGA 7 cut(s) 488, 971, 1092, 1383, 1398, 1930, 2006
AsuII TTCGAA 1 cut(s) 17
AvaI CYCGRG 1 cut(s) 1541
AvaII GGWCC 6 cut(s) 699, 934, 1183, 1292, 1736, 2942
AxyI CCTNAGG 2 cut(s) 324, 1287
BaeGI GKGCMC 1 cut(s) 2227
BamHI GGATCC 2 cut(s) 386, 2665
BanI GGYRCC 1 cut(s) 2482
BanII GRGCYC 1 cut(s) 1286
BauI CACGAG 1 cut(s) 2141
BbsI GAAGAC 1 cut(s) 1893
Bbv12I GWGCWC 1 cut(s) 2227
BbvI GCAGC 4 cut(s) 1501, 1558, 1945, 2071
BccI CCATC 5 cut(s) 182, 647, 2030, 2749, 2827
BceAI ACGGC 1 cut(s) 2264
BcgI CGANNNNNNTGC 2 cut(s) 2692, 2726
BciT130I CCWGG 1 cut(s) 1690
BciVI GTATCC 1 cut(s) 2498
BclI TGATCA 1 cut(s) 789
BcoDI GTCTC 3 cut(s) 90, 435, 2860
BfaI CTAG 6 cut(s) 24, 1143, 1673, 1916, 2033, 2973
BfmI CTRYAG 3 cut(s) 396, 1104, 3023
BfoI RGCGCY 1 cut(s) 2726
BfrI CTTAAG 1 cut(s) 1469
BfuI GTATCC 1 cut(s) 2498
BglII AGATCT 2 cut(s) 337, 2671
BisI GCNGC 4 cut(s) 1490, 1547, 1959, 2060
BlpI GCTNAGC 1 cut(s) 2418
BlsI GCNGC 4 cut(s) 1491, 1548, 1960, 2061
Bme1390I CCNGG 1 cut(s) 1690
Bme18I GGWCC 6 cut(s) 699, 934, 1183, 1292, 1736, 2942
BmeT110I CYCGRG 1 cut(s) 1541
BmgBI CACGTC 1 cut(s) 732
BmiI GGNNCC 7 cut(s) 388, 659, 745, 1293, 2087, 2484, 2667
BmrFI CCNGG 1 cut(s) 1690
BmrI ACTGGG 1 cut(s) 84
BmsI GCATC 3 cut(s) 1641, 1832, 2833
BmuI ACTGGG 1 cut(s) 84
BpiI GAAGAC 1 cut(s) 1893
BpmI CTGGAG 3 cut(s) 131, 1050, 1818
Bpu10I CCTNAGC 1 cut(s) 938
Bpu1102I GCTNAGC 1 cut(s) 2418
Bpu14I TTCGAA 1 cut(s) 17
BpuEI CTTGAG 3 cut(s) 998, 1300, 1430
BsaAI YACGTR 1 cut(s) 2605
BsaBI GATNNNNATC 2 cut(s) 2382, 2670
BsaI GGTCTC 1 cut(s) 90
BsaJI CCNNGG 4 cut(s) 544, 735, 1166, 1540
BsaXI ACNNNNNCTCC 4 cut(s) 541, 571, 2591, 2621
Bsc4I CCNNNNNNNGG 8 cut(s) 420, 645, 1109, 1400, 1724, 2620, 2675, 3016
Bse1I ACTGG 7 cut(s) 79, 316, 701, 720, 1033, 2176, 2267
Bse21I CCTNAGG 2 cut(s) 324, 1287
Bse3DI GCAATG 3 cut(s) 241, 480, 2502
Bse8I GATNNNNATC 2 cut(s) 2382, 2670
BseBI CCWGG 1 cut(s) 1690
BseDI CCNNGG 4 cut(s) 544, 735, 1166, 1540
BseGI GGATG 5 cut(s) 1251, 2431, 2848, 2986, 2995
BseJI GATNNNNATC 2 cut(s) 2382, 2670
BseLI CCNNNNNNNGG 8 cut(s) 420, 645, 1109, 1400, 1724, 2620, 2675, 3016
BseMI GCAATG 3 cut(s) 241, 480, 2502
BseMII CTCAG 7 cut(s) 315, 486, 681, 857, 929, 1278, 2767
BseNI ACTGG 7 cut(s) 79, 316, 701, 720, 1033, 2176, 2267
BseRI GAGGAG 1 cut(s) 2612
BseSI GKGCMC 1 cut(s) 2227
BseXI GCAGC 4 cut(s) 1501, 1558, 1945, 2071
BsgI GTGCAG 1 cut(s) 1508
Bsh1236I CGCG 1 cut(s) 411
BshFI GGCC 6 cut(s) 529, 744, 1458, 1788, 2011, 2624
BshNI GGYRCC 1 cut(s) 2482
BsiHKAI GWGCWC 1 cut(s) 2227
BsiHKCI CYCGRG 1 cut(s) 1541
BslFI GGGAC 2 cut(s) 1305, 2971
BslI CCNNNNNNNGG 8 cut(s) 420, 645, 1109, 1400, 1724, 2620, 2675, 3016
BsmAI GTCTC 3 cut(s) 90, 435, 2860
BsmFI GGGAC 2 cut(s) 1305, 2971
BsmI GAATGC 1 cut(s) 2395
BsnI GGCC 6 cut(s) 529, 744, 1458, 1788, 2011, 2624
Bso31I GGTCTC 1 cut(s) 90
BsoBI CYCGRG 1 cut(s) 1541
Bsp119I TTCGAA 1 cut(s) 17
Bsp1286I GDGCHC 2 cut(s) 1286, 2227
Bsp1407I TGTACA 1 cut(s) 2529
Bsp1720I GCTNAGC 1 cut(s) 2418
Bsp19I CCATGG 1 cut(s) 735
BspACI CCGC 3 cut(s) 1095, 1426, 1436
BspANI GGCC 6 cut(s) 529, 744, 1458, 1788, 2011, 2624
BspCNI CTCAG 7 cut(s) 316, 487, 680, 858, 930, 1279, 2766
BspFNI CGCG 1 cut(s) 411
BspHI TCATGA 1 cut(s) 2317
BspLI GGNNCC 7 cut(s) 388, 659, 745, 1293, 2087, 2484, 2667
BspPI GGATC 9 cut(s) 202, 381, 394, 817, 1105, 1970, 2391, 2660, 2673
BspT104I TTCGAA 1 cut(s) 17
BspT107I GGYRCC 1 cut(s) 2482
BspTI CTTAAG 1 cut(s) 1469
BspTNI GGTCTC 1 cut(s) 90
BsrBI CCGCTC 1 cut(s) 1438
BsrDI GCAATG 3 cut(s) 241, 480, 2502
BsrGI TGTACA 1 cut(s) 2529
BsrI ACTGG 7 cut(s) 79, 316, 701, 720, 1033, 2176, 2267
BssECI CCNNGG 4 cut(s) 544, 735, 1166, 1540
BssSI CACGAG 1 cut(s) 2141
BssT1I CCWWGG 2 cut(s) 544, 735
Bst2BI CACGAG 1 cut(s) 2141
Bst2UI CCWGG 1 cut(s) 1690
Bst4CI ACNGT 8 cut(s) 251, 299, 1108, 1296, 1759, 2090, 2534, 2814
Bst6I CTCTTC 3 cut(s) 537, 1266, 2427
BstAFI CTTAAG 1 cut(s) 1469
BstAPI GCANNNNNTGC 1 cut(s) 1619
BstAUI TGTACA 1 cut(s) 2529
BstBAI YACGTR 1 cut(s) 2605
BstBI TTCGAA 1 cut(s) 17
BstC8I GCNNGC 5 cut(s) 1175, 1426, 1466, 1810, 2783
BstDSI CCRYGG 2 cut(s) 735, 1166
BstENI CCTNNNNNAGG 1 cut(s) 3014
BstF5I GGATG 5 cut(s) 1251, 2431, 2848, 2986, 2995
BstFNI CGCG 1 cut(s) 411
BstH2I RGCGCY 1 cut(s) 2726
BstHHI GCGC 2 cut(s) 413, 2725
BstMAI GTCTC 3 cut(s) 90, 435, 2860
BstMWI GCNNNNNNNGC 4 cut(s) 408, 1170, 1619, 2048
BstNI CCWGG 1 cut(s) 1690
BstNSI RCATGY 3 cut(s) 1006, 1468, 2785
BstSCI CCNGG 1 cut(s) 1688
BstSFI CTRYAG 3 cut(s) 396, 1104, 3023
BstSLI GKGCMC 1 cut(s) 2227
BstUI CGCG 1 cut(s) 411
BstV1I GCAGC 4 cut(s) 1501, 1558, 1945, 2071
BstV2I GAAGAC 1 cut(s) 1893
BstX2I RGATCY 6 cut(s) 337, 386, 2383, 2665, 2671, 3019
BstXI CCANNNNNNTGG 1 cut(s) 191
BstYI RGATCY 6 cut(s) 337, 386, 2383, 2665, 2671, 3019
Bsu36I CCTNAGG 2 cut(s) 324, 1287
BsuI GTATCC 1 cut(s) 2498
BsuRI GGCC 6 cut(s) 529, 744, 1458, 1788, 2011, 2624
BtgI CCRYGG 2 cut(s) 735, 1166
BtgZI GCGATG 1 cut(s) 2717
BtrI CACGTC 1 cut(s) 732
BtsCI GGATG 5 cut(s) 1251, 2431, 2848, 2986, 2995
BtsIMutI CAGTG 4 cut(s) 72, 1134, 1533, 2569
Cac8I GCNNGC 5 cut(s) 1175, 1426, 1466, 1810, 2783
CaiI CAGNNNCTG 1 cut(s) 2182
CciI TCATGA 1 cut(s) 2317
CfoI GCGC 2 cut(s) 413, 2725
Csp6I GTAC 7 cut(s) 295, 1037, 1267, 1338, 2027, 2530, 2602
CspCI CAANNNNNGTGG 2 cut(s) 1148, 1183
CviQI GTAC 7 cut(s) 295, 1037, 1267, 1338, 2027, 2530, 2602
DraI TTTAAA 2 cut(s) 924, 1587
Eam1104I CTCTTC 3 cut(s) 537, 1266, 2427
EarI CTCTTC 3 cut(s) 537, 1266, 2427
Eco130I CCWWGG 2 cut(s) 544, 735
Eco24I GRGCYC 1 cut(s) 1286
Eco31I GGTCTC 1 cut(s) 90
Eco32I GATATC 2 cut(s) 135, 2118
Eco47I GGWCC 6 cut(s) 699, 934, 1183, 1292, 1736, 2942
Eco81I CCTNAGG 2 cut(s) 324, 1287
Eco88I CYCGRG 1 cut(s) 1541
EcoNI CCTNNNNNAGG 1 cut(s) 3014
EcoO109I RGGNCCY 3 cut(s) 527, 743, 1183
EcoRI GAATTC 1 cut(s) 151
EcoRII CCWGG 1 cut(s) 1688
EcoRV GATATC 2 cut(s) 135, 2118
EcoT14I CCWWGG 2 cut(s) 544, 735
EcoT22I ATGCAT 2 cut(s) 822, 2288
EcoT38I GRGCYC 1 cut(s) 1286
ErhI CCWWGG 2 cut(s) 544, 735
FalI AAGNNNNNCTT 4 cut(s) 516, 548, 2901, 2933
FaqI GGGAC 2 cut(s) 1305, 2971
FauI CCCGC 2 cut(s) 1433, 1443
FbaI TGATCA 1 cut(s) 789
Fnu4HI GCNGC 4 cut(s) 1490, 1547, 1959, 2060
FokI GGATG 5 cut(s) 1258, 2438, 2855, 2982, 2993
FriOI GRGCYC 1 cut(s) 1286
Fsp4HI GCNGC 4 cut(s) 1490, 1547, 1959, 2060
FspBI CTAG 6 cut(s) 24, 1143, 1673, 1916, 2033, 2973
GlaI GCGC 2 cut(s) 412, 2724
GluI GCNGC 4 cut(s) 1490, 1547, 1959, 2060
GsuI CTGGAG 3 cut(s) 131, 1050, 1818
HaeII RGCGCY 1 cut(s) 2726
HaeIII GGCC 6 cut(s) 529, 744, 1458, 1788, 2011, 2624
HhaI GCGC 2 cut(s) 413, 2725
Hin6I GCGC 2 cut(s) 411, 2723
HinP1I GCGC 2 cut(s) 411, 2723
HincII GTYRAC 5 cut(s) 780, 954, 1118, 2101, 2307
HindII GTYRAC 5 cut(s) 780, 954, 1118, 2101, 2307
HindIII AAGCTT 3 cut(s) 199, 704, 2147
HpaI GTTAAC 2 cut(s) 780, 2101
HphI GGTGA 7 cut(s) 488, 971, 1092, 1383, 1398, 1930, 2006
HpyCH4III ACNGT 8 cut(s) 251, 299, 1108, 1296, 1759, 2090, 2534, 2814
HpyCH4IV ACGT 2 cut(s) 731, 2604
HpyF10VI GCNNNNNNNGC 4 cut(s) 408, 1170, 1619, 2048
HpySE526I ACGT 2 cut(s) 731, 2604
HspAI GCGC 2 cut(s) 411, 2723
Ksp22I TGATCA 1 cut(s) 789
KspAI GTTAAC 2 cut(s) 780, 2101
LmnI GCTCC 4 cut(s) 657, 1497, 1799, 2836
Lsp1109I GCAGC 4 cut(s) 1501, 1558, 1945, 2071
LweI GCATC 3 cut(s) 1641, 1832, 2833
MaeI CTAG 6 cut(s) 24, 1143, 1673, 1916, 2033, 2973
MaeII ACGT 2 cut(s) 731, 2604
MaeIII GTNAC 8 cut(s) 238, 277, 476, 1478, 1759, 1871, 1967, 2178
MbiI CCGCTC 1 cut(s) 1438
MfeI CAATTG 1 cut(s) 785
MflI RGATCY 6 cut(s) 337, 386, 2383, 2665, 2671, 3019
MhlI GDGCHC 2 cut(s) 1286, 2227
MlyI GAGTC 2 cut(s) 564, 2993
MmeI TCCRAC 3 cut(s) 486, 992, 1223
Mph1103I ATGCAT 2 cut(s) 822, 2288
MslI CAYNNNNRTG 2 cut(s) 290, 734
MspCI CTTAAG 1 cut(s) 1469
MspR9I CCNGG 1 cut(s) 1690
MunI CAATTG 1 cut(s) 785
Mva1269I GAATGC 1 cut(s) 2395
MvaI CCWGG 1 cut(s) 1690
MvnI CGCG 1 cut(s) 411
MwoI GCNNNNNNNGC 4 cut(s) 408, 1170, 1619, 2048
NcoI CCATGG 1 cut(s) 735
NlaIV GGNNCC 7 cut(s) 388, 659, 745, 1293, 2087, 2484, 2667
NmuCI GTSAC 4 cut(s) 238, 277, 476, 1759
NsiI ATGCAT 2 cut(s) 822, 2288
NspI RCATGY 3 cut(s) 1006, 1468, 2785
NspV TTCGAA 1 cut(s) 17
PaeI GCATGC 2 cut(s) 1468, 2785
PagI TCATGA 1 cut(s) 2317
PciI ACATGT 1 cut(s) 1002
PctI GAATGC 1 cut(s) 2395
PflMI CCANNNNNTGG 1 cut(s) 2675
PfoI TCCNGGA 1 cut(s) 1688
PkrI GCNGC 4 cut(s) 1491, 1548, 1960, 2061
PleI GAGTC 2 cut(s) 564, 2992
PpsI GAGTC 2 cut(s) 564, 2992
Ppu21I YACGTR 1 cut(s) 2605
PpuMI RGGWCCY 1 cut(s) 1183
PscI ACATGT 1 cut(s) 1002
PshBI ATTAAT 1 cut(s) 1227
Psp5II RGGWCCY 1 cut(s) 1183
Psp6I CCWGG 1 cut(s) 1688
PspGI CCWGG 1 cut(s) 1688
PspN4I GGNNCC 7 cut(s) 388, 659, 745, 1293, 2087, 2484, 2667
PspPPI RGGWCCY 1 cut(s) 1183
PsrI GAACNNNNNNTAC 2 cut(s) 2235, 2267
PstNI CAGNNNCTG 1 cut(s) 2182
PsuI RGATCY 6 cut(s) 337, 386, 2383, 2665, 2671, 3019
RsaI GTAC 7 cut(s) 296, 1038, 1268, 1339, 2028, 2531, 2603
RsaNI GTAC 7 cut(s) 295, 1037, 1267, 1338, 2027, 2530, 2602
RseI CAYNNNNRTG 2 cut(s) 290, 734
SatI GCNGC 4 cut(s) 1490, 1547, 1959, 2060
SchI GAGTC 2 cut(s) 564, 2993
ScrFI CCNGG 1 cut(s) 1690
SduI GDGCHC 2 cut(s) 1286, 2227
SfaNI GCATC 3 cut(s) 1641, 1832, 2833
SfcI CTRYAG 3 cut(s) 396, 1104, 3023
SfuI TTCGAA 1 cut(s) 17
SinI GGWCC 6 cut(s) 699, 934, 1183, 1292, 1736, 2942
SmiMI CAYNNNNRTG 2 cut(s) 290, 734
SmlI CTYRAG 4 cut(s) 977, 1279, 1445, 1469
SmoI CTYRAG 4 cut(s) 977, 1279, 1445, 1469
SphI GCATGC 2 cut(s) 1468, 2785
SsiI CCGC 3 cut(s) 1095, 1426, 1436
SspI AATATT 1 cut(s) 1600
SspMI CTAG 6 cut(s) 24, 1143, 1673, 1916, 2033, 2973
StyD4I CCNGG 1 cut(s) 1688
StyI CCWWGG 2 cut(s) 544, 735
TaaI ACNGT 8 cut(s) 251, 299, 1108, 1296, 1759, 2090, 2534, 2814
TaiI ACGT 2 cut(s) 734, 2607
TaqI TCGA 5 cut(s) 17, 445, 1861, 2166, 2455
TatI WGTACW 2 cut(s) 1337, 2529
TscAI CASTG 4 cut(s) 79, 1134, 1540, 2569
TseFI GTSAC 4 cut(s) 238, 277, 476, 1759
TseI GCWGC 4 cut(s) 1489, 1546, 1958, 2059
Tsp45I GTSAC 4 cut(s) 238, 277, 476, 1759
TspDTI ATGAA 9 cut(s) 818, 1140, 1262, 1294, 1619, 2208, 2306, 2334, 3006
TspGWI ACGGA 1 cut(s) 233
TspRI CASTG 4 cut(s) 79, 1134, 1540, 2569
Van91I CCANNNNNTGG 1 cut(s) 2675
Vha464I CTTAAG 1 cut(s) 1469
VneI GTGCAC 1 cut(s) 2223
VpaK11BI GGWCC 6 cut(s) 699, 934, 1183, 1292, 1736, 2942
VspI ATTAAT 1 cut(s) 1227
XagI CCTNNNNNAGG 1 cut(s) 3014
XbaI TCTAGA 1 cut(s) 1672
XceI RCATGY 3 cut(s) 1006, 1468, 2785
XspI CTAG 6 cut(s) 24, 1143, 1673, 1916, 2033, 2973
Zsp2I ATGCAT 2 cut(s) 822, 2288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.