Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0002164

Physical Location & Seq

Genomic Coordinates Forward (+)

33460184 .. 33468404

Gene Structure 8221 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1247100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2814 bp

ATGTCTTTTTCATTGCTGAAAACTCTCGAAACAATTTCTAGAAAATTACATAATCCTCACTGGAAAAATATCAGCCAAACTTCTTGCCAAGATGGTGGGGTAGGCTTCTTCAAATACTTCACTGCCCACATTTTTAGCAATGTCACTTGCAAGTGTTCCTTTGCAAACAACACTTGCCATGTCAGAATCATCAAGCTGAAGGGTCTGAATTTAACCGGAGTTATACCTGAGGAATTTGGGGACCTTACTCATTTGGAAGAAATTGATCTCACTCACAACTACATTAGTGGATCAATCCCTGCCAGCATTTCTCGGTGTCCCATTCGTTCTCTATATCTTTTAGGGAATCGACTCAGTGGTTCAATTCCTGCTGGTGACTTTACATTGCTCAGAGAGCTGGTGTTGGAAGATAATCAATTTGAAGGGCCTCTTCCGCAGAACCTCGGGAGGTTGACTCACTTGGAAAGACTTGATCTGCAAGGTACATCCATGGAAGGCCCTATTCCTTACACCATATCTTCGTTGAAATACTTGAAGGAATTGATGATATCAGATTTGAATGGTCCAAGTATGCCTTTTCCTACTTTGAAATATATGAAAAATATCCAGATAGTGATTTTGAGAAATTGCTCAATTAGTGGTACAATCCCAAATTACCTTGGCAAGCTGACAAAGCTATTAACATTAGACTTGAGCTTTAACAAGTTAACAGGAGAAATTCCACAGATGATTGAACGTGCTACCCATCTATCTTTCGTGTGTTTGAGCAACAACATATTGACCGGAGAAGTACCAATTGGGATATTGCAAAGCAAACGTGACCAGGATTTGTCATACAACAATTTTACGGGTTCACCTTCCGTCAGTTGCCAACATTTGACCGTGTGGTGCTTGAAGAAGGGCCTTCCTTGCTCCTCAAAACCGAAATACCATTCTATATTTATCAATTGTGGAGGGAGGAAGACCAAGTTTGATGGTCATACATATGAAGAGGATTTAAGCACTTTCGGACCATCAAGCTTTTTTTCGTCATCTCAAAAGTGGGGCTATAGCAGCACGGGGCTGTACATGCGCAAGCCTAATGCAAATTACATTGCAACAAATGCGCTATCTTTGAATATCACCGGTCCAGATTTTTATCAAACAGCGCGCATTGCTCCTGTATCACTCAGGTATTATGCCCTTTGCATGCTAAAAGGCAGGTACAAAGTGCGGCTCTACTTTTCCGAAATAATGTTCACAAATGACCAGACATTTAGCAGTCTCGGAAAACGCTTATTTGACATCTCAATTCAAGGGAATTTGGTCCAAAAAGACTTTAATATTATGGACGAAGCAGGAGGTGTTGGTAAGGGAATTGTCAAAGAGTTCGACAATGTTACTGTTCTTGGTAGCACACTAGAGATCCACTTATATTGGGCAGGAAAAGGAACTACCGCCATTCCTTATAGAGGTGTCTATGGGCCTCTCATATCTGCGATTAGTGTGACACCAAGTAAGTTTTGCCTATGTTGTAGAATATCTGCAAATAATCTCTCATCAATTAGATTTACATTTATTCTAATAAAATTATACTTTATATTGGCAGACTTTGATCCTCCAACAGAAAGTGTAAGCACCATACCGCATGGTAGGAGTTGTCTGCCTATAAGCACAATAGTTAAAATTTGGGGGTGCGTAGCAGTTGCCATCATATTAATAGTGCTCGTTATTCTTTGGATCAAGAGATGCCTCGTAGGACTACAGAAAACATTGGAGAATGATTTGAAAGGTGTGGAACTACAAGCTCGCAAGTTTACCTTTACGGAACTCAACCATGCCACAACCAACTTTGACCAAGCCAATAAGATTGGTAAAGGTGGTTTTGGTACTGTTTATAAGGGCATTCTTGCTGATGGAACCCAGATTGCTGTTAAGCAGCTTTCTCCCAATTCAAACCAAGGAAATCGTGAATTTTTTAATGAGATAGGGATGCTCTATGCTTTGCAACACCCTAATCTTGTATACCTATATGGATGTTGTATTGAAGAAAATCAGTTATTCCTTGTCTACGAGTACATGGAAAACAATAGCGTTTATGATGCATTGTTTGTAAGTCGGTTGGATTTGGATTGGCCAACGAGGCGTAAGATATGTGTTGGTATAGCAAAAGGTTTGGCTTACCTTCATGATGGATCAAGCTTGAGGGTTATTCATAGAGATATAAAGGCTTCGAACGTATTGCTTGATAGACATCTTAATCCGAAGATATCGGACTTTGGATTGGCTAAACTTGCCGAAGAGGATAAAACACATATAAGCACCAATCCTATTGGAACTATTGGCTACATTGCACCAGAATATGTAAATCAAGGTCATCTTACTGATAAAGCAGATGTTTACAGCTTTGGAATTGTTCTACTAGAGATTATTAGTGGGAAGAAGAAATCAAATCAAGGAGGATTCTATATTCTTCATTGGGCGCATGCTCTCAAATCGAAGGGGAATTTGATGAACTTAGTGGATCCAAGGTTGGGCTCAGAATTCAACAATGAAGAGATGATAGCTACAATCAATGTGGCTCTCCACTGCTGTAATTCAACTTCAAGGCTTAGGCCTACCATGTCTGCAGTTATGAGCATGCTTGAAGGTAAGGCTGTTGTTCAAGAGTTCGTCTTAGGCCCCAATCCCTCATCAAGTACTGAGATGGATGAAGTTTTCCAGTCTGTTTTGAGAGGGGGCGCTAGGACACTTGAAGGGCCATCGAATGATTCAGCCGCAGCTCTTGATGATATCCATCCAGTCAATCCTCATTCAGGCCACCAAGACAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_1 LRR_8 Malectin Pkinase Pkinase_Tyr

Protein Analysis

938

Amino Acids

104.05

Weight (kDa)

8.49

Isoelectric Point (pI)

36.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_8	PF13855	60 - 118	1e-05	Leucine rich repeat
LRR_14	PF23598	127 - 236	9.9e-09	Leucine-rich repeat region
Malectin	PF11721	313 - 494	1.6e-37	Malectin domain
Pkinase	PF00069	612 - 872	2.1e-48	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	614 - 875	1.1e-50	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002164)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53430 AT1G53430 AT1G53440
fragaria_vesca	FvH4_3g17940
malus_domestica	MD03G1247100.v1.1 MD03G1248200.v1.1 MD03G1248300.v1.1 MD03G1249100.v1.1 MD03G1249200.v1.1 MD11G1267500.v1.1
prunus_persica	Prupe.4G158000_v2.0.a1 Prupe.4G158000_v2.0.a1
pyrus_communis	pycom03g19620 pycom03g19750 pycom06g03840 pycom11g23530
rosa_chinensis	RchiOBHm_Chr5g0029911 RchiOBHm_Chr5g0029981
rosa_laevigata	RLG00000033201 RLG00000033206
rosa_multiflora	Rmu_sc0003368.1_g000014 Rmu_sc0004542.1_g000020 Rmu_sc0021047.1_g000001
rosa_roxburghii	Rroxscaffold_1G00050050
rosa_rugosa	Rorug05G0115300 Rorug05G0115400 Rorug05G0115500 Rorug05G0115600 Rorug05G0115700 Rorug05G0115800 Rorug05G0115900 Rorug05G0116000 Rorug05G0116100 Rorug05G0116200
rosa_samantha	Rh5AG208900 Rh5AG209100 Rh5BG207700 Rh5CG231100 Rh5CG231800 Rh5DG211900 Rh5DG212500
rosa_wichuraiana	Rw5G019010 Rw5G019050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1878
Acc16I	TGCGCA	1 cut(s)	1073
Acc36I	ACCTGC	1 cut(s)	1191
AccI	GTMKAC	2 cut(s)	2004, 2049
AccII	CGCG	1 cut(s)	1150
AciI	CCGC	5 cut(s)	434, 1213, 1437, 1625, 2757
AclWI	GGATC	7 cut(s)	298, 1399, 1589, 1727, 2182, 2498, 2511
AcoI	YGGCCR	1 cut(s)	2114
AcsI	RAATTY	8 cut(s)	208, 233, 717, 1300, 1665, 1952, 2485, 2522
AcuI	CTGAAG	1 cut(s)	218
AfaI	GTAC	8 cut(s)	484, 643, 792, 1067, 1205, 1870, 2057, 2680
AfiI	CCNNNNNNNGG	3 cut(s)	1451, 2513, 2795
AgeI	ACCGGT	1 cut(s)	1124
AjnI	CCWGG	1 cut(s)	822
AjuI	GAANNNNNNNTTGG	2 cut(s)	780, 812
Alw21I	GWGCWC	1 cut(s)	1707
Alw26I	GTCTC	1 cut(s)	1268
AlwI	GGATC	7 cut(s)	298, 1399, 1589, 1727, 2182, 2498, 2511
Ama87I	CYCGRG	1 cut(s)	443
AoxI	GGCC	9 cut(s)	425, 496, 901, 1463, 2114, 2594, 2659, 2738, 2797
ApeKI	GCWGC	3 cut(s)	1053, 1918, 2759
ApoI	RAATTY	8 cut(s)	208, 233, 717, 1300, 1665, 1952, 2485, 2522
AseI	ATTAAT	1 cut(s)	1697
AsiGI	ACCGGT	1 cut(s)	1124
AspLEI	GCGC	6 cut(s)	1074, 1108, 1150, 1152, 2464, 2723
AsuHPI	GGTGA	3 cut(s)	386, 846, 1114
AsuII	TTCGAA	1 cut(s)	2213
AvaI	CYCGRG	1 cut(s)	443
AvaII	GGWCC	5 cut(s)	241, 563, 1010, 1127, 1306
AxyI	CCTNAGG	1 cut(s)	228
BalI	TGGCCA	1 cut(s)	2116
BamHI	GGATCC	1 cut(s)	2503
BanII	GRGCYC	1 cut(s)	2519
BarI	GAAGNNNNNNTAC	2 cut(s)	2566, 2598
BbsI	GAAGAC	1 cut(s)	968
Bbv12I	GWGCWC	1 cut(s)	1707
BbvI	GCAGC	3 cut(s)	1065, 1930, 2771
BcgI	CGANNNNNNTGC	2 cut(s)	2203, 2237
BciT130I	CCWGG	1 cut(s)	824
BcoDI	GTCTC	1 cut(s)	1268
BfaI	CTAG	4 cut(s)	39, 1400, 2402, 2724
BfmI	CTRYAG	3 cut(s)	1048, 1742, 2607
BfoI	RGCGCY	1 cut(s)	2724
BfuAI	ACCTGC	1 cut(s)	1191
BglI	GCCNNNNNGGC	1 cut(s)	2122
BisI	GCNGC	5 cut(s)	1054, 1214, 1919, 2757, 2760
BlsI	GCNGC	5 cut(s)	1055, 1215, 1920, 2758, 2761
BmcAI	AGTACT	1 cut(s)	2680
Bme1390I	CCNGG	1 cut(s)	824
Bme18I	GGWCC	5 cut(s)	241, 563, 1010, 1127, 1306
BmeT110I	CYCGRG	1 cut(s)	443
BmiI	GGNNCC	4 cut(s)	242, 1900, 2505, 2662
BmrFI	CCNGG	1 cut(s)	824
BmsI	GCATC	3 cut(s)	1718, 1962, 2071
BpiI	GAAGAC	1 cut(s)	968
BplI	GAGNNNNNCTC	2 cut(s)	439, 471
Bpu10I	CCTNAGC	1 cut(s)	2591
Bpu14I	TTCGAA	1 cut(s)	2213
BpuEI	CTTGAG	2 cut(s)	712, 2203
BsaBI	GATNNNNATC	3 cut(s)	1137, 2232, 2775
BsaJI	CCNNGG	5 cut(s)	442, 489, 658, 1939, 2507
BsaWI	WCCGGW	3 cut(s)	215, 782, 1124
Bsc4I	CCNNNNNNNGG	3 cut(s)	1451, 2513, 2795
Bse118I	RCCGGY	1 cut(s)	1124
Bse1I	ACTGG	3 cut(s)	65, 2701, 2780
Bse21I	CCTNAGG	1 cut(s)	228
Bse3DI	GCAATG	6 cut(s)	11, 145, 383, 1092, 1152, 2328
Bse8I	GATNNNNATC	3 cut(s)	1137, 2232, 2775
BseBI	CCWGG	1 cut(s)	824
BseDI	CCNNGG	5 cut(s)	442, 489, 658, 1939, 2507
BseGI	GGATG	5 cut(s)	485, 1977, 2021, 2695, 2776
BseJI	GATNNNNATC	3 cut(s)	1137, 2232, 2775
BseLI	CCNNNNNNNGG	3 cut(s)	1451, 2513, 2795
BseMI	GCAATG	6 cut(s)	11, 145, 383, 1092, 1152, 2328
BseMII	CTCAG	6 cut(s)	219, 367, 403, 1183, 2532, 2673
BseNI	ACTGG	3 cut(s)	65, 2701, 2780
BsePI	GCGCGC	1 cut(s)	1148
BseRI	GAGGAG	1 cut(s)	904
BseXI	GCAGC	3 cut(s)	1065, 1930, 2771
Bsh1236I	CGCG	1 cut(s)	1150
BshFI	GGCC	9 cut(s)	427, 498, 903, 1465, 2116, 2596, 2661, 2740, 2799
BshTI	ACCGGT	1 cut(s)	1124
BsiHKAI	GWGCWC	1 cut(s)	1707
BsiHKCI	CYCGRG	1 cut(s)	443
BsiSI	CCGG	3 cut(s)	216, 783, 1125
BslFI	GGGAC	2 cut(s)	254, 303
BslI	CCNNNNNNNGG	3 cut(s)	1451, 2513, 2795
BsmAI	GTCTC	1 cut(s)	1268
BsmFI	GGGAC	2 cut(s)	254, 303
BsmI	GAATGC	1 cut(s)	1884
BsnI	GGCC	9 cut(s)	427, 498, 903, 1465, 2116, 2596, 2661, 2740, 2799
BsoBI	CYCGRG	1 cut(s)	443
Bsp119I	TTCGAA	1 cut(s)	2213
Bsp1286I	GDGCHC	2 cut(s)	1707, 2519
Bsp1407I	TGTACA	1 cut(s)	1065
Bsp143I	GATC	8 cut(s)	265, 290, 472, 1404, 1594, 1719, 2174, 2503
Bsp19I	CCATGG	1 cut(s)	489
BspACI	CCGC	5 cut(s)	434, 1213, 1437, 1625, 2757
BspANI	GGCC	9 cut(s)	427, 498, 903, 1465, 2116, 2596, 2661, 2740, 2799
BspCNI	CTCAG	6 cut(s)	220, 366, 402, 1182, 2531, 2674
BspFNI	CGCG	1 cut(s)	1150
BspHI	TCATGA	1 cut(s)	2167
BspLI	GGNNCC	4 cut(s)	242, 1900, 2505, 2662
BspMAI	CTGCAG	1 cut(s)	2611
BspMI	ACCTGC	1 cut(s)	1191
BspPI	GGATC	7 cut(s)	298, 1399, 1589, 1727, 2182, 2498, 2511
BspT104I	TTCGAA	1 cut(s)	2213
BsrDI	GCAATG	6 cut(s)	11, 145, 383, 1092, 1152, 2328
BsrFI	RCCGGY	1 cut(s)	1124
BsrGI	TGTACA	1 cut(s)	1065
BsrI	ACTGG	3 cut(s)	65, 2701, 2780
BssAI	RCCGGY	1 cut(s)	1124
BssECI	CCNNGG	5 cut(s)	442, 489, 658, 1939, 2507
BssHII	GCGCGC	1 cut(s)	1148
BssMI	GATC	8 cut(s)	265, 290, 472, 1404, 1594, 1719, 2174, 2503
BssNAI	GTATAC	1 cut(s)	2005
BssT1I	CCWWGG	4 cut(s)	489, 658, 1939, 2507
Bst1107I	GTATAC	1 cut(s)	2005
Bst2UI	CCWGG	1 cut(s)	824
Bst4CI	ACNGT	3 cut(s)	883, 1384, 1873
Bst6I	CTCTTC	4 cut(s)	435, 984, 2274, 2529
BstAPI	GCANNNNNTGC	1 cut(s)	1103
BstAUI	TGTACA	1 cut(s)	1065
BstBI	TTCGAA	1 cut(s)	2213
BstC8I	GCNNGC	8 cut(s)	304, 665, 1076, 1150, 1190, 1789, 2466, 2621
BstDEI	CTNAG	9 cut(s)	228, 353, 389, 1169, 2497, 2518, 2591, 2656, 2682
BstDSI	CCRYGG	1 cut(s)	489
BstENI	CCTNNNNNAGG	2 cut(s)	1449, 2793
BstF5I	GGATG	5 cut(s)	485, 1977, 2021, 2695, 2776
BstFNI	CGCG	1 cut(s)	1150
BstH2I	RGCGCY	1 cut(s)	2724
BstHHI	GCGC	6 cut(s)	1074, 1108, 1150, 1152, 2464, 2723
BstKTI	GATC	8 cut(s)	268, 293, 475, 1407, 1597, 1722, 2177, 2506
BstMAI	GTCTC	1 cut(s)	1268
BstMBI	GATC	8 cut(s)	265, 290, 472, 1404, 1594, 1719, 2174, 2503
BstNI	CCWGG	1 cut(s)	824
BstNSI	RCATGY	4 cut(s)	1072, 1192, 2468, 2623
BstSCI	CCNGG	1 cut(s)	822
BstSFI	CTRYAG	3 cut(s)	1048, 1742, 2607
BstUI	CGCG	1 cut(s)	1150
BstV1I	GCAGC	3 cut(s)	1065, 1930, 2771
BstV2I	GAAGAC	1 cut(s)	968
BstX2I	RGATCY	2 cut(s)	1404, 2503
BstXI	CCANNNNNNTGG	1 cut(s)	95
BstYI	RGATCY	2 cut(s)	1404, 2503
BstZ17I	GTATAC	1 cut(s)	2005
Bsu36I	CCTNAGG	1 cut(s)	228
BsuRI	GGCC	9 cut(s)	427, 498, 903, 1465, 2116, 2596, 2661, 2740, 2799
BtgI	CCRYGG	1 cut(s)	489
BtsCI	GGATG	5 cut(s)	485, 1977, 2021, 2695, 2776
BtsI	GCAGTG	2 cut(s)	120, 2566
BtsIMutI	CAGTG	4 cut(s)	58, 120, 361, 2566
BveI	ACCTGC	1 cut(s)	1191
Cac8I	GCNNGC	8 cut(s)	304, 665, 1076, 1150, 1190, 1789, 2466, 2621
CciI	TCATGA	1 cut(s)	2167
CfoI	GCGC	6 cut(s)	1074, 1108, 1150, 1152, 2464, 2723
Cfr10I	RCCGGY	1 cut(s)	1124
Csp6I	GTAC	8 cut(s)	483, 642, 791, 1066, 1204, 1869, 2056, 2679
CspAI	ACCGGT	1 cut(s)	1124
CspCI	CAANNNNNGTGG	4 cut(s)	1397, 1432, 2538, 2573
CviQI	GTAC	8 cut(s)	483, 642, 791, 1066, 1204, 1869, 2056, 2679
DdeI	CTNAG	9 cut(s)	228, 353, 389, 1169, 2497, 2518, 2591, 2656, 2682
DpnI	GATC	8 cut(s)	267, 292, 474, 1406, 1596, 1721, 2176, 2505
DpnII	GATC	8 cut(s)	265, 290, 472, 1404, 1594, 1719, 2174, 2503
EaeI	YGGCCR	1 cut(s)	2114
Eam1104I	CTCTTC	4 cut(s)	435, 984, 2274, 2529
EarI	CTCTTC	4 cut(s)	435, 984, 2274, 2529
Eco130I	CCWWGG	4 cut(s)	489, 658, 1939, 2507
Eco147I	AGGCCT	1 cut(s)	2596
Eco24I	GRGCYC	1 cut(s)	2519
Eco32I	GATATC	3 cut(s)	549, 2250, 2773
Eco47I	GGWCC	5 cut(s)	241, 563, 1010, 1127, 1306
Eco57I	CTGAAG	1 cut(s)	218
Eco81I	CCTNAGG	1 cut(s)	228
Eco88I	CYCGRG	1 cut(s)	443
EcoNI	CCTNNNNNAGG	2 cut(s)	1449, 2793
EcoO109I	RGGNCCY	5 cut(s)	241, 425, 497, 901, 2660
EcoRI	GAATTC	1 cut(s)	2522
EcoRII	CCWGG	1 cut(s)	822
EcoRV	GATATC	3 cut(s)	549, 2250, 2773
EcoT14I	CCWWGG	4 cut(s)	489, 658, 1939, 2507
EcoT22I	ATGCAT	1 cut(s)	2086
EcoT38I	GRGCYC	1 cut(s)	2519
ErhI	CCWWGG	4 cut(s)	489, 658, 1939, 2507
FaqI	GGGAC	2 cut(s)	254, 303
FauNDI	CATATG	1 cut(s)	985
FblI	GTMKAC	2 cut(s)	2004, 2049
Fnu4HI	GCNGC	5 cut(s)	1054, 1214, 1919, 2757, 2760
FokI	GGATG	5 cut(s)	472, 1984, 2028, 2702, 2763
FriOI	GRGCYC	1 cut(s)	2519
Fsp4HI	GCNGC	5 cut(s)	1054, 1214, 1919, 2757, 2760
FspBI	CTAG	4 cut(s)	39, 1400, 2402, 2724
FspI	TGCGCA	1 cut(s)	1073
GlaI	GCGC	6 cut(s)	1073, 1107, 1149, 1151, 2463, 2722
GluI	GCNGC	5 cut(s)	1054, 1214, 1919, 2757, 2760
HaeII	RGCGCY	1 cut(s)	2724
HaeIII	GGCC	9 cut(s)	427, 498, 903, 1465, 2116, 2596, 2661, 2740, 2799
HapII	CCGG	3 cut(s)	216, 783, 1125
HhaI	GCGC	6 cut(s)	1074, 1108, 1150, 1152, 2464, 2723
Hin6I	GCGC	6 cut(s)	1072, 1106, 1148, 1150, 2462, 2721
HinP1I	GCGC	6 cut(s)	1072, 1106, 1148, 1150, 2462, 2721
HincII	GTYRAC	2 cut(s)	453, 708
HindII	GTYRAC	2 cut(s)	453, 708
HindIII	AAGCTT	2 cut(s)	1018, 2179
HinfI	GANTC	6 cut(s)	186, 346, 351, 454, 2442, 2750
HpaI	GTTAAC	1 cut(s)	708
HpaII	CCGG	3 cut(s)	216, 783, 1125
HphI	GGTGA	3 cut(s)	386, 846, 1114
Hpy166II	GTNNAC	8 cut(s)	453, 708, 854, 1239, 1797, 2005, 2050, 2380
Hpy8I	GTNNAC	8 cut(s)	453, 708, 854, 1239, 1797, 2005, 2050, 2380
HpyCH4III	ACNGT	3 cut(s)	883, 1384, 1873
HpyCH4IV	ACGT	3 cut(s)	736, 817, 2217
HpyF3I	CTNAG	9 cut(s)	228, 353, 389, 1169, 2497, 2518, 2591, 2656, 2682
HpySE526I	ACGT	3 cut(s)	736, 817, 2217
HspAI	GCGC	6 cut(s)	1072, 1106, 1148, 1150, 2462, 2721
KspAI	GTTAAC	1 cut(s)	708
Kzo9I	GATC	8 cut(s)	265, 290, 472, 1404, 1594, 1719, 2174, 2503
LmnI	GCTCC	2 cut(s)	917, 1162
Lsp1109I	GCAGC	3 cut(s)	1065, 1930, 2771
LweI	GCATC	3 cut(s)	1718, 1962, 2071
MaeI	CTAG	4 cut(s)	39, 1400, 2402, 2724
MaeII	ACGT	3 cut(s)	736, 817, 2217
MaeIII	GTNAC	5 cut(s)	142, 374, 818, 1378, 1486
MalI	GATC	8 cut(s)	267, 292, 474, 1406, 1596, 1721, 2176, 2505
MboI	GATC	8 cut(s)	265, 290, 472, 1404, 1594, 1719, 2174, 2503
MfeI	CAATTG	2 cut(s)	795, 946
MflI	RGATCY	2 cut(s)	1404, 2503
MhlI	GDGCHC	2 cut(s)	1707, 2519
MlsI	TGGCCA	1 cut(s)	2116
MluNI	TGGCCA	1 cut(s)	2116
MlyI	GAGTC	2 cut(s)	345, 448
MmeI	TCCRAC	3 cut(s)	384, 1625, 2082
Mox20I	TGGCCA	1 cut(s)	2116
Mph1103I	ATGCAT	1 cut(s)	2086
MscI	TGGCCA	1 cut(s)	2116
MslI	CAYNNNNRTG	1 cut(s)	984
Msp20I	TGGCCA	1 cut(s)	2116
MspI	CCGG	3 cut(s)	216, 783, 1125
MspR9I	CCNGG	1 cut(s)	824
MunI	CAATTG	2 cut(s)	795, 946
Mva1269I	GAATGC	1 cut(s)	1884
MvaI	CCWGG	1 cut(s)	824
MvnI	CGCG	1 cut(s)	1150
NcoI	CCATGG	1 cut(s)	489
NdeI	CATATG	1 cut(s)	985
NdeII	GATC	8 cut(s)	265, 290, 472, 1404, 1594, 1719, 2174, 2503
NlaIV	GGNNCC	4 cut(s)	242, 1900, 2505, 2662
NmuCI	GTSAC	4 cut(s)	142, 374, 818, 1486
NsbI	TGCGCA	1 cut(s)	1073
NsiI	ATGCAT	1 cut(s)	2086
NspI	RCATGY	4 cut(s)	1072, 1192, 2468, 2623
NspV	TTCGAA	1 cut(s)	2213
PaeI	GCATGC	3 cut(s)	1192, 2468, 2623
PagI	TCATGA	1 cut(s)	2167
PauI	GCGCGC	1 cut(s)	1148
PceI	AGGCCT	1 cut(s)	2596
PctI	GAATGC	1 cut(s)	1884
PfeI	GAWTC	4 cut(s)	186, 346, 2442, 2750
PinAI	ACCGGT	1 cut(s)	1124
PkrI	GCNGC	5 cut(s)	1055, 1215, 1920, 2758, 2761
PleI	GAGTC	2 cut(s)	345, 448
PpsI	GAGTC	2 cut(s)	345, 448
PpuMI	RGGWCCY	1 cut(s)	241
PshBI	ATTAAT	1 cut(s)	1697
PsiI	TTATAA	1 cut(s)	1878
Psp5II	RGGWCCY	1 cut(s)	241
Psp6I	CCWGG	1 cut(s)	822
PspGI	CCWGG	1 cut(s)	822
PspN4I	GGNNCC	4 cut(s)	242, 1900, 2505, 2662
PspPPI	RGGWCCY	1 cut(s)	241
PstI	CTGCAG	1 cut(s)	2611
PsuI	RGATCY	2 cut(s)	1404, 2503
PteI	GCGCGC	1 cut(s)	1148
RsaI	GTAC	8 cut(s)	484, 643, 792, 1067, 1205, 1870, 2057, 2680
RsaNI	GTAC	8 cut(s)	483, 642, 791, 1066, 1204, 1869, 2056, 2679
RseI	CAYNNNNRTG	1 cut(s)	984
SatI	GCNGC	5 cut(s)	1054, 1214, 1919, 2757, 2760
Sau3AI	GATC	8 cut(s)	265, 290, 472, 1404, 1594, 1719, 2174, 2503
ScaI	AGTACT	1 cut(s)	2680
SchI	GAGTC	2 cut(s)	345, 448
ScrFI	CCNGG	1 cut(s)	824
SduI	GDGCHC	2 cut(s)	1707, 2519
SfaNI	GCATC	3 cut(s)	1718, 1962, 2071
SfcI	CTRYAG	3 cut(s)	1048, 1742, 2607
SfuI	TTCGAA	1 cut(s)	2213
SinI	GGWCC	5 cut(s)	241, 563, 1010, 1127, 1306
SmiMI	CAYNNNNRTG	1 cut(s)	984
SmlI	CTYRAG	2 cut(s)	691, 2182
SmoI	CTYRAG	2 cut(s)	691, 2182
SphI	GCATGC	3 cut(s)	1192, 2468, 2623
SseBI	AGGCCT	1 cut(s)	2596
SsiI	CCGC	5 cut(s)	434, 1213, 1437, 1625, 2757
SspI	AATATT	1 cut(s)	1324
SspMI	CTAG	4 cut(s)	39, 1400, 2402, 2724
StuI	AGGCCT	1 cut(s)	2596
StyD4I	CCNGG	1 cut(s)	822
StyI	CCWWGG	4 cut(s)	489, 658, 1939, 2507
TaaI	ACNGT	3 cut(s)	883, 1384, 1873
TaiI	ACGT	3 cut(s)	739, 820, 2220
TaqI	TCGA	6 cut(s)	27, 349, 1371, 2213, 2477, 2744
TatI	WGTACW	3 cut(s)	1065, 2055, 2678
TauI	GCSGC	2 cut(s)	1216, 2759
TfiI	GAWTC	4 cut(s)	186, 346, 2442, 2750
TscAI	CASTG	4 cut(s)	65, 127, 361, 2573
TseFI	GTSAC	4 cut(s)	142, 374, 818, 1486
TseI	GCWGC	3 cut(s)	1053, 1918, 2759
Tsp45I	GTSAC	4 cut(s)	142, 374, 818, 1486
TspDTI	ATGAA	8 cut(s)	611, 1002, 2156, 2183, 2444, 2507, 2547, 2706
TspGWI	ACGGA	2 cut(s)	850, 1820
TspRI	CASTG	4 cut(s)	65, 127, 361, 2573
VpaK11BI	GGWCC	5 cut(s)	241, 563, 1010, 1127, 1306
VspI	ATTAAT	1 cut(s)	1697
XagI	CCTNNNNNAGG	2 cut(s)	1449, 2793
XapI	RAATTY	8 cut(s)	208, 233, 717, 1300, 1665, 1952, 2485, 2522
XbaI	TCTAGA	1 cut(s)	38
XceI	RCATGY	4 cut(s)	1072, 1192, 2468, 2623
XmiI	GTMKAC	2 cut(s)	2004, 2049
XspI	CTAG	4 cut(s)	39, 1400, 2402, 2724
ZrmI	AGTACT	1 cut(s)	2680
Zsp2I	ATGCAT	1 cut(s)	2086

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.