Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0002164

Physical Location & Seq

Genomic Coordinates Forward (+)

24472845 .. 24481699

Gene Structure 8855 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000033206

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3018 bp

ATGTACGGTAATTTAACTGGTATTCTACCTGAGGAATTTGGAGATCTTACATATCTACAAGAACTTGATCTCACTCTTAACTACATCAATGGATCCATTCCTACAGGCATTTCTCGCGCCCCTCTTAGGAATCTGTCTCTTACAGGAAATCGACTAAGTGGTTCTATTCCAAAGGAAATTGGTGACATTGCTACTCTTACTAAGCTTTTGTTGGAAGATAATCAACTTGAAGGGCCTCTTCCTCAAAACCTTGGGAGTTTGAGTTCCTTGAATAGACTCCTTCTTTCTGCGAATAACTTTACAGGAACAATTCCAGAATCATTTGGAAATCTAAAGAACTTAACCGATTTTAGGATAGATGGGAGCCGAATCTCAGGAAAAATACCTACTTTTATTGGAAACTGGACCAAGCTTGACAGACTGGATATGCAAGGCACGTCCATGGAAGGCCCCATTCCTTCCACCATATCTTTGCTGACAAAGTTAACTCAATTGATCATATCTGATTTGAATGGATCAAGTATGCATTTTCCTAAATTGGAGGATTTGAAAATTTTGAAAACATTGGACCTGAGCTTTAACAAATTGACAGGTGAAGTTCCAACAACCCTTAAAAATCTTCCCCTTCAATACATGTTTTTGACCAACAATTTACTGACTGGAGAAGTACCAAGCTGGATATTGGGCAACAAATATCACTTTGATTTGTCTTACAACAATTTTAGCGGATCACCTACAGTTGGTTGTCAACACTTATCAGTGAATTTAATTTCTAGCTTTTCATCTTCACAAAGCCCGTGGTGCTTGCAGAAGGACCTCCCTTGTTCCACAAAAGCCAAATACTATTCTGTGTTTATTAATTGTGGGGGGGGTCGGATGAAATTCAATGGTAATCAGTACGAAGAGGACTTGAGCCCTGAGGGACCGTCAGACTTCATTTCCTCATTTGAGAAGTGGGGTTATAGCAGTACAGGGATTTACATGGGGAAGGAAAAAGCAGATTACACAGCAACAAACACATCACCTCTTAATGGTTCACCAATTTATCAAACTGCCCGCCTTGCCCCGCTCTCACTCAAGTATTATGGCCTTTGCATGCTTAAGGGTAGTTACAAAGTGCAGCTCCACTTCGCTGAAATAAAGTATTCAGATAATGGCACATTCACTGACCTCGGGCAGCGTATATTTGATGTGTCAATTCAAGGAAATTTGGTTTTAAAGGATTTCAATATTATGAAGGAAGCAGGAGGTGCTGGTAAAGGCATCGTCAGAGAATTTAATGTCCTTGTTAATGGTAGCACTCTAGAAATCCACTTATCCTGGAAAGGAAAAGGAACTAATGCAATCCCTGATAGGGGTGGCTATGGACCTCTTATATCTGCAATTACTGTGACACCAAATTTTAAGGTTGATATTGGCCTATCTGCTGGAGCTATTGCTGGCATTGTAGTAGCATCATGTGTGTTGATTGTATTCATTTTGGCAGTCCTTCGATATATGGGTTACTTGGGGGGCGAAGACCTTGAAGATAAAGGCTTGAGAATGAGAAGTTTCGAGCCTAGGACCCAAATTAAAGCTTTGATACCATGGTTAATCAGAAGATTCAAACTGGTTAAATGTGGGGTACTACTAGATGGTGCTGTAATTGCTGTTAAGCAGCTCTCATCTAAATCAAAGCAAGGGAACCGTGAATTTGTTAACGAGATAGGCATGATATCTGCTTTGCAACACCCAAATCTCGTGAAGCTTTTTGGTTGCTGTATCGAAGGAAACCAGTTTTTGCTTATATATGAATACATGGAAAACAATAGTCTTGCCCGTGCACTTTTTGGTAAAGCCTGCAACGTTATCAGCATAATGTTAAATTCAGAGGTCTTGTATTTCATGTTCTTGTCCCCTATAATTGTAGGTCGTGAAGAACAACGGCTACACTTGGACTGGAAAACAAGAAAGAAGATATGCATGGGGATAGCGAGAGGGTTGACCTATCTTCATGAAGAATCAAGGTTGAAAATTGTTCACAGGGATATAAAGGCAACTAATGTCCTGCTTGATAAGGATCTGAATGCCAAAATATCTGATTTTGGTTTAGCTAAGCTGGATGAAGAAGAGAACTCACATATCAGCACCCGAATAGCTGGAACAATAGGCTATATGGCACCTGAATACGCAATGAGGGGATACTTGACTGACAAAGCAGATGTGTACAGTTTTGGTATTGTTGCCTTAGAAATAGTCAGTGGAAAGAGCAACACAAATTACAGACCAAAGGAGGAGTACGTGTATCTCCTTGATTGGGCCTATGTCCTACAAGAGCAAGGAAATATGTTAGAACTTGTGGATCCAGATCTTGGTTCAAACTACTCCAAAGAAGAGGCGATGACAATGCTTAACTTGGCGCTCTTATGCACCAATCCATCTCCCACTCTCAGACCACCCATGTCATCTGTCGTAAGCATGCTTGAAGGTAAAAGTCCGATTCATGCACCGTTAATCAAGGGCGACACGATGGAGCAGGATGCGAGGTTCAAAGCCTTTGAGAGACTATCACAAGACAGCCAAACGCATGATGTGATTAACAAGGTCAGACCAGAGTTCATAGATGGCCCCGGAGAAAAACTACTCATGCAGCTTCAAGCAACTTGGGAGGCCAAGATGATGCAAGCCGGTGTTGTTGGTACGCCTGCTGTGGATCTCAATTTCAACCACAACCGCCTCTACCGGAAACTGGAGACAACTTGTTCAATACTATTCCTACCGGAGCACCATACATGCAACCTCCTTATCCTTGGATTACCTCTCAAACATATCAGACCGCTGGTGACGGGTTTATACCCCAGCAAGTGGAGCCGGAGGGGTCTTCAAGTTGAGGTTAGTGGAGGGAGCAGTAGTACTTATGTGAAGGTAGAGGGGGATGATGAGCCCCCATTGATTGAAGACGACGACGATGATTTGGATGATGTGGAGGAGGGAAAGGAGGAGCATGAACACACAGAATTTGGTGTTGGCTCAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRR_1 LRR_8 Malectin Pkinase Pkinase_Tyr

Protein Analysis

1006

Amino Acids

111.47

Weight (kDa)

5.82

Isoelectric Point (pI)

31.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Malectin	PF11721	284 - 462	7.8e-37	Malectin domain
Pkinase	PF00069	544 - 761	9.1e-37	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	548 - 820	2.3e-42	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002164)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53430 AT1G53430 AT1G53440
fragaria_vesca	FvH4_3g17940
malus_domestica	MD03G1247100.v1.1 MD03G1248200.v1.1 MD03G1248300.v1.1 MD03G1249100.v1.1 MD03G1249200.v1.1 MD11G1267500.v1.1
prunus_persica	Prupe.4G158000_v2.0.a1 Prupe.4G158000_v2.0.a1
pyrus_communis	pycom03g19620 pycom03g19750 pycom06g03840 pycom11g23530
rosa_chinensis	RchiOBHm_Chr5g0029911 RchiOBHm_Chr5g0029981
rosa_laevigata	RLG00000033201 RLG00000033206
rosa_multiflora	Rmu_sc0003368.1_g000014 Rmu_sc0004542.1_g000020 Rmu_sc0021047.1_g000001
rosa_roxburghii	Rroxscaffold_1G00050050
rosa_rugosa	Rorug05G0115300 Rorug05G0115400 Rorug05G0115500 Rorug05G0115600 Rorug05G0115700 Rorug05G0115800 Rorug05G0115900 Rorug05G0116000 Rorug05G0116100 Rorug05G0116200
rosa_samantha	Rh5AG208900 Rh5AG209100 Rh5BG207700 Rh5CG231100 Rh5CG231800 Rh5DG211900 Rh5DG212500
rosa_wichuraiana	Rw5G019010 Rw5G019050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	2158
AccB7I	CCANNNNNTGG	2 cut(s)	2351, 2844
AccBSI	CCGCTC	1 cut(s)	1069
AccII	CGCG	1 cut(s)	117
AciI	CCGC	5 cut(s)	726, 1057, 1067, 2713, 2816
AclI	AACGTT	1 cut(s)	1845
AclWI	GGATC	8 cut(s)	87, 100, 523, 736, 2067, 2336, 2349, 2700
AfaI	GTAC	9 cut(s)	5, 669, 899, 970, 1626, 2207, 2279, 2680, 2893
AflII	CTTAAG	1 cut(s)	1100
AflIII	ACRYGT	2 cut(s)	633, 2280
AjiI	CACGTC	1 cut(s)	438
AjnI	CCWGG	1 cut(s)	1319
AjuI	GAANNNNNNNTTGG	1 cut(s)	2988
AloI	GAACNNNNNNTCC	4 cut(s)	247, 279, 2510, 2542
Alw21I	GWGCWC	2 cut(s)	1825, 2766
Alw26I	GTCTC	3 cut(s)	141, 2536, 2726
Alw44I	GTGCAC	1 cut(s)	1821
AlwI	GGATC	8 cut(s)	87, 100, 523, 736, 2067, 2336, 2349, 2700
Ama87I	CYCGRG	1 cut(s)	1172
AoxI	GGCC	7 cut(s)	233, 448, 1087, 1417, 2298, 2605, 2649
ApaLI	GTGCAC	1 cut(s)	1821
ApeKI	GCWGC	4 cut(s)	1120, 1177, 1657, 2629
AseI	ATTAAT	1 cut(s)	858
AspA2I	CCTAGG	1 cut(s)	1559
AspLEI	GCGC	2 cut(s)	119, 2401
AsuC2I	CCSGG	1 cut(s)	2610
AsuHPI	GGTGA	6 cut(s)	194, 605, 723, 1014, 1029, 2833
AvaI	CYCGRG	1 cut(s)	1172
AvaII	GGWCC	6 cut(s)	405, 568, 814, 923, 1367, 1563
AvrII	CCTAGG	1 cut(s)	1559
AxyI	CCTNAGG	2 cut(s)	30, 918
BaeGI	GKGCMC	1 cut(s)	1825
BaeI	ACNNNNGTAYC	2 cut(s)	1574, 1607
BamHI	GGATCC	2 cut(s)	92, 2341
BanI	GGYRCC	1 cut(s)	2158
BanII	GRGCYC	2 cut(s)	917, 2925
BauI	CACGAG	1 cut(s)	1739
BbsI	GAAGAC	3 cut(s)	1524, 2852, 2943
Bbv12I	GWGCWC	2 cut(s)	1825, 2766
BbvI	GCAGC	4 cut(s)	1132, 1189, 1669, 2641
BccI	CCATC	5 cut(s)	353, 1628, 2425, 2503, 2597
BceAI	ACGGC	1 cut(s)	1940
BcgI	CGANNNNNNTGC	2 cut(s)	2368, 2402
BciT130I	CCWGG	1 cut(s)	1321
BciVI	GTATCC	1 cut(s)	2174
BclI	TGATCA	1 cut(s)	495
BcnI	CCSGG	1 cut(s)	2610
BcoDI	GTCTC	3 cut(s)	141, 2536, 2726
BfaI	CTAG	4 cut(s)	774, 1304, 1560, 1631
BfmI	CTRYAG	2 cut(s)	102, 735
BfoI	RGCGCY	1 cut(s)	2402
BfrI	CTTAAG	1 cut(s)	1100
BfuI	GTATCC	1 cut(s)	2174
BglII	AGATCT	2 cut(s)	43, 2347
BisI	GCNGC	4 cut(s)	1121, 1178, 1658, 2630
BlnI	CCTAGG	1 cut(s)	1559
BlpI	GCTNAGC	1 cut(s)	2094
BlsI	GCNGC	4 cut(s)	1122, 1179, 1659, 2631
BmcAI	AGTACT	1 cut(s)	2893
Bme1390I	CCNGG	2 cut(s)	1321, 2610
Bme18I	GGWCC	6 cut(s)	405, 568, 814, 923, 1367, 1563
BmeT110I	CYCGRG	1 cut(s)	1172
BmgBI	CACGTC	1 cut(s)	438
BmrFI	CCNGG	2 cut(s)	1321, 2610
BmsI	GCATC	4 cut(s)	1272, 1463, 2509, 2649
BpiI	GAAGAC	3 cut(s)	1524, 2852, 2943
BpmI	CTGGAG	3 cut(s)	681, 1449, 2750
Bpu10I	CCTNAGC	1 cut(s)	572
Bpu1102I	GCTNAGC	1 cut(s)	2094
BpuEI	CTTGAG	3 cut(s)	931, 1061, 1558
BpuMI	CCSGG	1 cut(s)	2610
BsaAI	YACGTR	1 cut(s)	2281
BsaBI	GATNNNNATC	2 cut(s)	2058, 2346
BsaJI	CCNNGG	8 cut(s)	250, 441, 797, 1171, 1559, 1586, 2608, 2788
BsaWI	WCCGGW	2 cut(s)	2721, 2758
BsaXI	ACNNNNNCTCC	6 cut(s)	247, 277, 2267, 2297, 2875, 2905
Bse118I	RCCGGY	1 cut(s)	2666
Bse1I	ACTGG	8 cut(s)	22, 407, 426, 664, 1614, 1774, 1943, 2733
Bse21I	CCTNAGG	2 cut(s)	30, 918
Bse3DI	GCAATG	2 cut(s)	186, 2178
Bse8I	GATNNNNATC	2 cut(s)	2058, 2346
BseBI	CCWGG	1 cut(s)	1321
BseDI	CCNNGG	8 cut(s)	250, 441, 797, 1171, 1559, 1586, 2608, 2788
BseGI	GGATG	5 cut(s)	882, 2107, 2524, 2920, 2962
BseJI	GATNNNNATC	2 cut(s)	2058, 2346
BseMI	GCAATG	2 cut(s)	186, 2178
BseMII	CTCAG	5 cut(s)	21, 387, 563, 909, 2443
BseNI	ACTGG	8 cut(s)	22, 407, 426, 664, 1614, 1774, 1943, 2733
BseRI	GAGGAG	3 cut(s)	2288, 2981, 2993
BseSI	GKGCMC	1 cut(s)	1825
BseXI	GCAGC	4 cut(s)	1132, 1189, 1669, 2641
BseYI	CCCAGC	1 cut(s)	2837
BsgI	GTGCAG	1 cut(s)	1139
Bsh1236I	CGCG	1 cut(s)	117
BshFI	GGCC	7 cut(s)	235, 450, 1089, 1419, 2300, 2607, 2651
BshNI	GGYRCC	1 cut(s)	2158
BsiHKAI	GWGCWC	2 cut(s)	1825, 2766
BsiHKCI	CYCGRG	1 cut(s)	1172
BsiSI	CCGG	5 cut(s)	2610, 2667, 2722, 2759, 2851
BslFI	GGGAC	2 cut(s)	936, 1879
BsmAI	GTCTC	3 cut(s)	141, 2536, 2726
BsmFI	GGGAC	2 cut(s)	936, 1879
BsmI	GAATGC	1 cut(s)	2071
BsnI	GGCC	7 cut(s)	235, 450, 1089, 1419, 2300, 2607, 2651
BsoBI	CYCGRG	1 cut(s)	1172
Bsp1286I	GDGCHC	4 cut(s)	917, 1825, 2766, 2925
Bsp1407I	TGTACA	1 cut(s)	2205
Bsp1720I	GCTNAGC	1 cut(s)	2094
Bsp19I	CCATGG	2 cut(s)	441, 1586
BspACI	CCGC	5 cut(s)	726, 1057, 1067, 2713, 2816
BspANI	GGCC	7 cut(s)	235, 450, 1089, 1419, 2300, 2607, 2651
BspCNI	CTCAG	5 cut(s)	22, 386, 564, 910, 2442
BspFNI	CGCG	1 cut(s)	117
BspHI	TCATGA	1 cut(s)	1993
BspPI	GGATC	8 cut(s)	87, 100, 523, 736, 2067, 2336, 2349, 2700
BspT107I	GGYRCC	1 cut(s)	2158
BspTI	CTTAAG	1 cut(s)	1100
BsrBI	CCGCTC	1 cut(s)	1069
BsrDI	GCAATG	2 cut(s)	186, 2178
BsrFI	RCCGGY	1 cut(s)	2666
BsrGI	TGTACA	1 cut(s)	2205
BsrI	ACTGG	8 cut(s)	22, 407, 426, 664, 1614, 1774, 1943, 2733
BssAI	RCCGGY	1 cut(s)	2666
BssECI	CCNNGG	8 cut(s)	250, 441, 797, 1171, 1559, 1586, 2608, 2788
BssSI	CACGAG	1 cut(s)	1739
BssT1I	CCWWGG	5 cut(s)	250, 441, 1559, 1586, 2788
Bst2BI	CACGAG	1 cut(s)	1739
Bst2UI	CCWGG	1 cut(s)	1321
Bst4CI	ACNGT	7 cut(s)	8, 739, 927, 1390, 1688, 2210, 2490
Bst6I	CTCTTC	4 cut(s)	243, 897, 2103, 2367
BstAFI	CTTAAG	1 cut(s)	1100
BstAPI	GCANNNNNTGC	1 cut(s)	1250
BstAUI	TGTACA	1 cut(s)	2205
BstBAI	YACGTR	1 cut(s)	2281
BstC8I	GCNNGC	8 cut(s)	806, 1057, 1097, 1441, 1840, 2459, 2664, 2685
BstDSI	CCRYGG	3 cut(s)	441, 797, 1586
BstF5I	GGATG	5 cut(s)	882, 2107, 2524, 2920, 2962
BstFNI	CGCG	1 cut(s)	117
BstH2I	RGCGCY	1 cut(s)	2402
BstHHI	GCGC	2 cut(s)	119, 2401
BstMAI	GTCTC	3 cut(s)	141, 2536, 2726
BstMWI	GCNNNNNNNGC	6 cut(s)	114, 801, 1061, 1250, 1646, 2847
BstNI	CCWGG	1 cut(s)	1321
BstNSI	RCATGY	4 cut(s)	637, 1099, 2461, 2775
BstSCI	CCNGG	2 cut(s)	1319, 2608
BstSFI	CTRYAG	2 cut(s)	102, 735
BstSLI	GKGCMC	1 cut(s)	1825
BstUI	CGCG	1 cut(s)	117
BstV1I	GCAGC	4 cut(s)	1132, 1189, 1669, 2641
BstV2I	GAAGAC	3 cut(s)	1524, 2852, 2943
BstX2I	RGATCY	6 cut(s)	43, 92, 2059, 2341, 2347, 2692
BstYI	RGATCY	6 cut(s)	43, 92, 2059, 2341, 2347, 2692
Bsu36I	CCTNAGG	2 cut(s)	30, 918
BsuI	GTATCC	1 cut(s)	2174
BsuRI	GGCC	7 cut(s)	235, 450, 1089, 1419, 2300, 2607, 2651
BtgI	CCRYGG	3 cut(s)	441, 797, 1586
BtgZI	GCGATG	1 cut(s)	2393
BtrI	CACGTC	1 cut(s)	438
BtsCI	GGATG	5 cut(s)	882, 2107, 2524, 2920, 2962
BtsIMutI	CAGTG	3 cut(s)	765, 1164, 2245
Cac8I	GCNNGC	8 cut(s)	806, 1057, 1097, 1441, 1840, 2459, 2664, 2685
CciI	TCATGA	1 cut(s)	1993
CfoI	GCGC	2 cut(s)	119, 2401
Cfr10I	RCCGGY	1 cut(s)	2666
Csp6I	GTAC	9 cut(s)	4, 668, 898, 969, 1625, 2206, 2278, 2679, 2892
CspCI	CAANNNNNGTGG	2 cut(s)	779, 814
CviQI	GTAC	9 cut(s)	4, 668, 898, 969, 1625, 2206, 2278, 2679, 2892
DraI	TTTAAA	1 cut(s)	1218
Eam1104I	CTCTTC	4 cut(s)	243, 897, 2103, 2367
EarI	CTCTTC	4 cut(s)	243, 897, 2103, 2367
Eco130I	CCWWGG	5 cut(s)	250, 441, 1559, 1586, 2788
Eco24I	GRGCYC	2 cut(s)	917, 2925
Eco32I	GATATC	1 cut(s)	1716
Eco47I	GGWCC	6 cut(s)	405, 568, 814, 923, 1367, 1563
Eco81I	CCTNAGG	2 cut(s)	30, 918
Eco88I	CYCGRG	1 cut(s)	1172
EcoO109I	RGGNCCY	4 cut(s)	233, 449, 814, 1563
EcoRII	CCWGG	1 cut(s)	1319
EcoRV	GATATC	1 cut(s)	1716
EcoT14I	CCWWGG	5 cut(s)	250, 441, 1559, 1586, 2788
EcoT22I	ATGCAT	2 cut(s)	528, 1964
EcoT38I	GRGCYC	2 cut(s)	917, 2925
ErhI	CCWWGG	5 cut(s)	250, 441, 1559, 1586, 2788
FalI	AAGNNNNNCTT	2 cut(s)	222, 254
FaqI	GGGAC	2 cut(s)	936, 1879
FauI	CCCGC	2 cut(s)	1064, 1074
FbaI	TGATCA	1 cut(s)	495
Fnu4HI	GCNGC	4 cut(s)	1121, 1178, 1658, 2630
FokI	GGATG	5 cut(s)	889, 2114, 2531, 2927, 2969
FriOI	GRGCYC	2 cut(s)	917, 2925
Fsp4HI	GCNGC	4 cut(s)	1121, 1178, 1658, 2630
FspBI	CTAG	4 cut(s)	774, 1304, 1560, 1631
GlaI	GCGC	2 cut(s)	118, 2400
GluI	GCNGC	4 cut(s)	1121, 1178, 1658, 2630
GsaI	CCCAGC	1 cut(s)	2841
GsuI	CTGGAG	3 cut(s)	681, 1449, 2750
HaeII	RGCGCY	1 cut(s)	2402
HaeIII	GGCC	7 cut(s)	235, 450, 1089, 1419, 2300, 2607, 2651
HapII	CCGG	5 cut(s)	2610, 2667, 2722, 2759, 2851
HhaI	GCGC	2 cut(s)	119, 2401
Hin6I	GCGC	2 cut(s)	117, 2399
HinP1I	GCGC	2 cut(s)	117, 2399
HincII	GTYRAC	4 cut(s)	486, 749, 1699, 1983
HindII	GTYRAC	4 cut(s)	486, 749, 1699, 1983
HindIII	AAGCTT	4 cut(s)	203, 410, 1575, 1745
HinfI	GANTC	7 cut(s)	130, 276, 317, 369, 1602, 2000, 2479
HpaI	GTTAAC	2 cut(s)	486, 1699
HpaII	CCGG	5 cut(s)	2610, 2667, 2722, 2759, 2851
HphI	GGTGA	6 cut(s)	194, 605, 723, 1014, 1029, 2833
Hpy166II	GTNNAC	8 cut(s)	486, 749, 1037, 1699, 1823, 1983, 2020, 2206
Hpy188III	TCNNGA	7 cut(s)	314, 375, 1304, 1741, 1913, 1994, 2345
Hpy8I	GTNNAC	8 cut(s)	486, 749, 1037, 1699, 1823, 1983, 2020, 2206
Hpy99I	CGWCG	2 cut(s)	2945, 2948
HpyCH4III	ACNGT	7 cut(s)	8, 739, 927, 1390, 1688, 2210, 2490
HpyCH4IV	ACGT	3 cut(s)	437, 1845, 2280
HpyF10VI	GCNNNNNNNGC	6 cut(s)	114, 801, 1061, 1250, 1646, 2847
HpySE526I	ACGT	3 cut(s)	437, 1845, 2280
HspAI	GCGC	2 cut(s)	117, 2399
Ksp22I	TGATCA	1 cut(s)	495
KspAI	GTTAAC	2 cut(s)	486, 1699
LmnI	GCTCC	8 cut(s)	363, 1128, 1430, 2512, 2761, 2847, 2883, 2980
Lsp1109I	GCAGC	4 cut(s)	1132, 1189, 1669, 2641
LweI	GCATC	4 cut(s)	1272, 1463, 2509, 2649
MaeI	CTAG	4 cut(s)	774, 1304, 1560, 1631
MaeII	ACGT	3 cut(s)	437, 1845, 2280
MaeIII	GTNAC	5 cut(s)	182, 1109, 1390, 1502, 2821
MbiI	CCGCTC	1 cut(s)	1069
MfeI	CAATTG	1 cut(s)	491
MflI	RGATCY	6 cut(s)	43, 92, 2059, 2341, 2347, 2692
MhlI	GDGCHC	4 cut(s)	917, 1825, 2766, 2925
MlyI	GAGTC	1 cut(s)	270
MmeI	TCCRAC	3 cut(s)	192, 626, 854
Mph1103I	ATGCAT	2 cut(s)	528, 1964
MslI	CAYNNNNRTG	1 cut(s)	440
MspA1I	CMGCKG	1 cut(s)	2818
MspCI	CTTAAG	1 cut(s)	1100
MspI	CCGG	5 cut(s)	2610, 2667, 2722, 2759, 2851
MspR9I	CCNGG	2 cut(s)	1321, 2610
MunI	CAATTG	1 cut(s)	491
Mva1269I	GAATGC	1 cut(s)	2071
MvaI	CCWGG	1 cut(s)	1321
MvnI	CGCG	1 cut(s)	117
MwoI	GCNNNNNNNGC	6 cut(s)	114, 801, 1061, 1250, 1646, 2847
NciI	CCSGG	1 cut(s)	2610
NcoI	CCATGG	2 cut(s)	441, 1586
NmuCI	GTSAC	3 cut(s)	182, 1390, 2821
NsiI	ATGCAT	2 cut(s)	528, 1964
NspI	RCATGY	4 cut(s)	637, 1099, 2461, 2775
PaeI	GCATGC	2 cut(s)	1099, 2461
PagI	TCATGA	1 cut(s)	1993
PciI	ACATGT	1 cut(s)	633
PctI	GAATGC	1 cut(s)	2071
PfeI	GAWTC	6 cut(s)	130, 317, 369, 1602, 2000, 2479
PflMI	CCANNNNNTGG	2 cut(s)	2351, 2844
PfoI	TCCNGGA	1 cut(s)	1319
PkrI	GCNGC	4 cut(s)	1122, 1179, 1659, 2631
PleI	GAGTC	1 cut(s)	270
PpsI	GAGTC	1 cut(s)	270
Ppu21I	YACGTR	1 cut(s)	2281
PpuMI	RGGWCCY	2 cut(s)	814, 1563
PscI	ACATGT	1 cut(s)	633
PshBI	ATTAAT	1 cut(s)	858
Psp1406I	AACGTT	1 cut(s)	1845
Psp5II	RGGWCCY	2 cut(s)	814, 1563
Psp6I	CCWGG	1 cut(s)	1319
PspFI	CCCAGC	1 cut(s)	2837
PspGI	CCWGG	1 cut(s)	1319
PspPPI	RGGWCCY	2 cut(s)	814, 1563
PsrI	GAACNNNNNNTAC	4 cut(s)	1871, 1903, 1911, 1943
PsuI	RGATCY	6 cut(s)	43, 92, 2059, 2341, 2347, 2692
RsaI	GTAC	9 cut(s)	5, 669, 899, 970, 1626, 2207, 2279, 2680, 2893
RsaNI	GTAC	9 cut(s)	4, 668, 898, 969, 1625, 2206, 2278, 2679, 2892
RseI	CAYNNNNRTG	1 cut(s)	440
SatI	GCNGC	4 cut(s)	1121, 1178, 1658, 2630
ScaI	AGTACT	1 cut(s)	2893
SchI	GAGTC	1 cut(s)	270
ScrFI	CCNGG	2 cut(s)	1321, 2610
SduI	GDGCHC	4 cut(s)	917, 1825, 2766, 2925
SfaNI	GCATC	4 cut(s)	1272, 1463, 2509, 2649
SfcI	CTRYAG	2 cut(s)	102, 735
SinI	GGWCC	6 cut(s)	405, 568, 814, 923, 1367, 1563
SmiMI	CAYNNNNRTG	1 cut(s)	440
SmlI	CTYRAG	4 cut(s)	910, 1076, 1100, 1537
SmoI	CTYRAG	4 cut(s)	910, 1076, 1100, 1537
SphI	GCATGC	2 cut(s)	1099, 2461
SsiI	CCGC	5 cut(s)	726, 1057, 1067, 2713, 2816
SspI	AATATT	1 cut(s)	1231
SspMI	CTAG	4 cut(s)	774, 1304, 1560, 1631
StyD4I	CCNGG	2 cut(s)	1319, 2608
StyI	CCWWGG	5 cut(s)	250, 441, 1559, 1586, 2788
TaaI	ACNGT	7 cut(s)	8, 739, 927, 1390, 1688, 2210, 2490
TaiI	ACGT	3 cut(s)	440, 1848, 2283
TaqI	TCGA	4 cut(s)	151, 1492, 1554, 1764
TatI	WGTACW	3 cut(s)	968, 2205, 2891
TfiI	GAWTC	6 cut(s)	130, 317, 369, 1602, 2000, 2479
TscAI	CASTG	3 cut(s)	765, 1171, 2245
TseFI	GTSAC	3 cut(s)	182, 1390, 2821
TseI	GCWGC	4 cut(s)	1120, 1177, 1657, 2629
Tsp45I	GTSAC	3 cut(s)	182, 1390, 2821
TspRI	CASTG	3 cut(s)	765, 1171, 2245
Van91I	CCANNNNNTGG	2 cut(s)	2351, 2844
Vha464I	CTTAAG	1 cut(s)	1100
VneI	GTGCAC	1 cut(s)	1821
VpaK11BI	GGWCC	6 cut(s)	405, 568, 814, 923, 1367, 1563
VspI	ATTAAT	1 cut(s)	858
XbaI	TCTAGA	1 cut(s)	1303
XceI	RCATGY	4 cut(s)	637, 1099, 2461, 2775
XmaJI	CCTAGG	1 cut(s)	1559
XspI	CTAG	4 cut(s)	774, 1304, 1560, 1631
ZrmI	AGTACT	1 cut(s)	2893
Zsp2I	ATGCAT	2 cut(s)	528, 1964

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.