AT1G61210

May participate in a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001097
Physical Location & Seq
Forward (+)
22564359 .. 22572029
7671 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G61210.3

Sequence Viewer

Length: 3546 bp
ATGGCGAAGCGTGGTTATAAGCTACAGGAGTTTTTGGCACATTCTGCAAATGTGAATTGTCTAAGTATCGGAAAGAAGACATCACGGCTCTTTATAACTGGTGGTGATGATTACAAAGTCAATCTTTGGGCGATTGGCAAGCCTACTTCTTTAATGAGTCTGTGTGGACATACTAGTGCAGTGGATTCAGTAGCTTTTGACTCCGCTGAAGTTTTGGTACTTGCTGGAGCTTCTTCGGGTGTGATAAAGCTGTGGGATGTTGAAGAAGCAAAGATGGTTCGAGCTTTTACTGGACATAGATCCAATTGTTCTGCTGTTGAATTTCATCCCTTTGGTGAATTTTTGGCATCGGGATCTAGTGACGCGAATCTAAAGATTTGGGACATCAGGAAGAAGGGATGCATACAGACGTACAAGGGTCATTCTCGTGGCATCAGCACTATCAGGTTTACTCCCGATGGTCGTTGGGTAGTCTCCGGAGGACTTGACAATGTTGTAAAGGTATGGGATTTGACTGCTGGAAAGCTTTTGCATGAATTTAAGTTTCATGAAGGACCTATCCGTTCGCTAGACTTCCACCCGCTTGAGTTCCTCCTAGCCACAGGTTCTGCTGACAGGACTGTAAAGTTCTGGGATTTGGAAACGTTTGAATTGATTGGATCTACCCGACCAGAGGCTACTGGAGTTCGTTCAATCAAATTTCATCCGGATGGGCGAACCTTATTTTGTGGTTTGGATGACAGCTTAAAGGTTTATTCATGGGAACCTGTGGTTTGCCATGATGGTGTTGATATGGGATGGTCAACGCTTGGTGACTTATGCATCAGCGAAGGAAAGCTCCTGGGTTGTTCATACTACCAAAATTCAGTAGGGATTTGGGTCTCAGATATATCACAGATTGAGCCATACGGCATTGGATCTGCGGATAAAAAAGAGTGCGTGGAGAAAATACTCAGTGCTCTGGATGATCAGTCCTTCGATAGAATAAAGAGTACCCCAAGGCGCAGCTCATCTCCAGATTATGAGACGAAAGAGATCAAAAACATATACATCGACTCTACAGGCGGAAATTCGGCTGTTGCACATAAGTCAGGATCTCTAAGTACTCCAGCAACTTCTACTGGACAAGCAGGGGATAATAAGTCTTTAGTAGTGCATAGTGTTGTTCCACGGGATAGTGATATTGGGAAAGACTCTTCTGATTCAGGAAAGGAATCTATCACTTTCTCAAAAACAAAGCCAGGCATGCTGTTGAGACCGGCTTATGTAAGAAAGACGCCAACAAAGTTTGATGAAACAAAGAAGCAGTCAGTGGCTGTTGGATATCTAAAGAAAAGTGGATTGGATGGTGAGAAGAAACTAGATACTGAGACGGCGTTTGATTCAGAAATGAGTGGTAGGAACCCATATGATGCCGACGATTCTATCATCAAGAGTATCACCAACAAGTTTGAACAAGCTTTATTACCAGAATCACCGACTGATGAAGCAAAATGTATGCTGCTGAAACCGCCTCGTGTACAGAGATCACCAAGTACTAAGTACAATGAGGCAAGATGGGCGACATCTACTGATTCTGGAGCTTTAGACAGTAAAAAGAATGGGTTAGAGTCCTCAAGAGACATGGACTTGCCAACAGGGCTCAGGGATGACAGAGGTAGTAACCCATGTGAGGAAGATATCGAGAACAAGAGCATTTCAAGTAGGTCAGAAAGAGTTCTGTCACCAGAAAAAGCTGGTGATGAACTAAAAAGCCTTGAATCCCCTGGAGGTAGCAAAGAATCAAAATCTGTGAAGGTTGTTAGAGGAGTAAAAGTTGTTTCGGGAAGGACACGGTCATTGGTTGAGAGGTTTGAAAGAGGAGAAAAAATTACTCATTCTGAAGATAAAGCAGCCAGTGCAACAGTAGTTCATAGCTCTAATTCTGTAGAGGAAGAGCCCCTGACAGCATCGGTACAGACAGTTAGCATGATGCCTACCCAGGTAATGCCAGTTAAACTTGATCAGGCAACTAACTCGACCACAGTTGACGTTCCTGTGTTATCAACACGACGAACTAAGAGTACTCCAGTCAGAGTAATGCCTGTAGTACTCGGTCGGGACACTAGCATGGCAACTGATACGCCTCCTGTAACATCAACCCGACCTGATAGGACTTCAGCTACTAATTTGACATCTGATGTGTCTGGTGTAACATCAAAACGACAAACTAGGACTTCTCCAGCCCCCGTCATGCCAATGATACTCAATCAGACAACAAAAATGAAATCTGATGAGCCTTCTATAACATCAACATGGCCTGATAGGACTTCGGCTACTGATTTGACATCTGATGTGTCTGGTGTAATATCATCACGACAAACTAGGACTTCTCCAGCACCGGTCATGCCAATGAAACTCAATCAGAAAACAAAAATAAAATCTGATGAGCCTCCTATAACATCAACACGGCCTGATAGGCCTTCAGCTACTAATTTGACATCTGATGAGTCTCCTGTAACATCAACACGACAAGCAAAGACTTCTCCAGCCCCAGTTACGCCTGTGATACTAAATCAGCGTCAAACAACTAACATGAAATCTGATGAGCCTCCTGTAATATCAACAAGACCACTTAGGACCTCTTCGGCACGAGTAATGCCTGTGATACTCAATCAAGCATCTACCACGTATGATGAGCGTCCTTTATCATCTTCACGATCGGCTAGAACTTCTCCTGCCCGTATAATGCCTATGAAACTCAATCAAGCAGATAATATGCCATCGTATGAGCCTCCAGTTGCATTAACACGATCAGCTAGGAACTCTCCCGCCCGTGTAATTCCTGTGAAACTTAATCAAGCAACAAATGTGACAGCTGATGCATCTCATATAAGATCCAGGCAAAGATTTAGTCCAACTCAAACGCTGGCAACACCTGCAGTATTTGATCAGGTGACTGATATGACACTGGATGAGACTACAAAAACTCAACAATCTTCTGATATCTTGACCCAGAAGGAAGAGCCTCAGATATCTGGGAGAGAGGATGATGGTGACATTTGGGAGATTTTAATGCGAACTCATAGTGAGGTCTTAAACACTCTTCAATCACGGTTGACAAAATTACAGATTGTAAGACATTTCTGGGAACGTAGTGATATTAAAGGCGCGATTGCGGCCTTAAGAAAACTGTCAGATCACTCTGTTCAAGCAGATGTGATCAATATTCTAACAGACAAAACAGAGATTTTAACATTAGATTTATTTTCTCAATTAGCACCCGTCCTCACAGGCTTATTGGGCAGCAAGACTGAGAGGCCTGTAAATGTCTCCTTGGAAATGCTCTTGAAGCTTGTAGCAGTGTTTGGCACAGTTATACAATCAACTGTTTCAGCACGACGAGTTGTCGGCGTTGATCTTCATGCAGAGGAAAGGTTACAGATCTGCCAAAGCTGTTCTGCGGAATTGCAAAAGGTTCAGAAGATTCTTCCACTACTGACAAGAAGAGGTGGTCTTATAGCAAGAAAGGCACAAGAACTAAACCTAGTTCTTCAAACACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Katanin_con80 WD40

Protein Analysis

1181

Amino Acids

129.17

Weight (kDa)

8.77

Isoelectric Point (pI)

49.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 3 - 141 1e-24 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 6 - 109 1.6e-16 WDR5 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 6 - 171 1e-12 MABP1/WDR62 second WD40 domain
Beta-prop_TEP1_2nd PF25047 9 - 107 9.7e-10 TEP-1 second beta-propeller
WD40_Gbeta PF25391 11 - 134 1.1e-15 G protein beta WD-40 repeat protein
Beta-prop_WDR3_2nd PF25172 11 - 128 7.8e-12 WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 12 - 131 1.5e-11 WDR36/Utp21 second beta-propeller domain
Beta-prop_SPT8 PF23798 13 - 202 3.4e-08 SPT8 beta propeller
WD40_Prp19 PF24814 14 - 253 1e-46 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 15 - 95 1.6e-08 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 26 - 177 1.4e-17 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 29 - 132 3.4e-07 WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 38 - 169 3.6e-12 WDR36/Utp21 first beta-propeller
Beta-prop_Aladin PF25460 40 - 172 7.6e-08 Aladin seven-bladed propeller
WD40_CDC20-Fz PF24807 49 - 243 4.5e-32 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 51 - 173 1.1e-15 WDR3 second beta-propeller domain
EIF3I PF24805 52 - 138 9.6e-06 EIF3I
Beta-prop_CAF1B_HIR1 PF24105 53 - 137 8.9e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 57 - 200 2.8e-31 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 57 - 201 3.4e-22 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 72 - 140 1.3e-10 WDHD1 first WD40 domain
WD40_MABP1-WDR62_1st PF24780 74 - 216 8.4e-10 MABP1/WDR62 first WD40 domain
WD40 PF00400 92 - 128 3.3e-08 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 97 - 258 3.5e-35 WDR5 beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 99 - 161 8.3e-07 WDR90/POC16, second beta-propeller
WDR55 PF24796 102 - 247 1.1e-14 WDR55
Beta-prop_WDR3_2nd PF25172 114 - 221 2e-14 WDR3 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 114 - 188 1.8e-07 TEP-1 second beta-propeller
Beta-prop_SCAP PF24017 116 - 213 8.7e-06 SCAP Beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 118 - 252 2.9e-11 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 125 - 253 2.9e-26 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 126 - 254 3.6e-14 G protein beta WD-40 repeat protein
WD40 PF00400 134 - 170 4.6e-11 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 135 - 225 1.5e-09 CAF1B/HIR1 beta-propeller domain
Beta-prop_EML PF23409 138 - 242 1.7e-06 Echinoderm microtubule-associated protein first beta-propeller
EIF3I PF24805 139 - 249 7.6e-15 EIF3I
WD40_WDHD1_1st PF24817 145 - 253 6.5e-11 WDHD1 first WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 163 - 244 8.3e-08 WDR90/POC16, second beta-propeller
WD40 PF00400 174 - 212 1.5e-08 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 176 - 247 3e-10 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 189 - 247 3.1e-07 Echinoderm microtubule-associated protein second beta-propeller
Katanin_con80 PF13925 917 - 1074 1.6e-51 con80 domain of Katanin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 18, 95
AarI CACCTGC 1 cut(s) 2926
Acc36I ACCTGC 1 cut(s) 2926
AccII CGCG 2 cut(s) 365, 3152
AccIII TCCGGA 2 cut(s) 476, 706
AciI CCGC 8 cut(s) 204, 581, 923, 1065, 1511, 2811, 3158, 3443
AclI AACGTT 1 cut(s) 644
AclWI GGATC 6 cut(s) 294, 361, 667, 925, 1102, 2871
AcsI RAATTY 6 cut(s) 320, 338, 536, 698, 862, 1069
AcuI CTGAAG 4 cut(s) 228, 1902, 2142, 2448
AcyI GRCGYC 1 cut(s) 1277
AfiI CCNNNNNNNGG 2 cut(s) 673, 1672
AflII CTTAAG 1 cut(s) 3163
AgeI ACCGGT 1 cut(s) 2380
AhdI GACNNNNNGTC 1 cut(s) 3386
AhlI ACTAGT 1 cut(s) 173
AjnI CCWGG 5 cut(s) 840, 1240, 1765, 1980, 2879
AjuI GAANNNNNNNTTGG 2 cut(s) 1325, 1357
Alw21I GWGCWC 1 cut(s) 961
Alw26I GTCTC 9 cut(s) 478, 886, 1019, 1249, 1364, 1614, 2496, 2951, 3316
AlwI GGATC 6 cut(s) 294, 361, 667, 925, 1102, 2871
AlwNI CAGNNNCTG 2 cut(s) 608, 1316
Aor13HI TCCGGA 2 cut(s) 476, 706
AoxI GGCC 5 cut(s) 2297, 2450, 2459, 3159, 3299
ApeKI GCWGC 4 cut(s) 1005, 1501, 1892, 3285
ApoI RAATTY 6 cut(s) 320, 338, 536, 698, 862, 1069
ArsI GACNNNNNNTTYG 4 cut(s) 1292, 1324, 2878, 2910
AsiGI ACCGGT 1 cut(s) 2380
Asp700I GAANNNNTTC 1 cut(s) 1716
AspLEI GCGC 2 cut(s) 1005, 3152
AspS9I GGNCC 2 cut(s) 554, 2619
AvaII GGWCC 2 cut(s) 554, 2619
BanII GRGCYC 2 cut(s) 1644, 1941
BarI GAAGNNNNNNTAC 2 cut(s) 201, 233
BauI CACGAG 3 cut(s) 426, 1515, 2631
BbsI GAAGAC 1 cut(s) 83
Bbv12I GWGCWC 1 cut(s) 961
BbvI GCAGC 4 cut(s) 1017, 1488, 1904, 3297
BccI CCATC 9 cut(s) 268, 452, 704, 776, 792, 1340, 1551, 2770, 3026
BceAI ACGGC 4 cut(s) 101, 925, 1389, 2465
BcgI CGANNNNNNTGC 2 cut(s) 1998, 2032
BciT130I CCWGG 5 cut(s) 842, 1242, 1767, 1982, 2881
BclI TGATCA 4 cut(s) 967, 2002, 2929, 3201
BcoDI GTCTC 9 cut(s) 478, 886, 1019, 1249, 1364, 1614, 2496, 2951, 3316
BcuI ACTAGT 1 cut(s) 173
BfmI CTRYAG 5 cut(s) 23, 1059, 1926, 2085, 2919
BfrI CTTAAG 1 cut(s) 3163
BfuAI ACCTGC 1 cut(s) 2926
BglI GCCNNNNNGGC 2 cut(s) 1639, 2458
BglII AGATCT 1 cut(s) 3423
BisI GCNGC 5 cut(s) 1006, 1502, 1893, 3159, 3286
BlsI GCNGC 5 cut(s) 1007, 1503, 1894, 3160, 3287
BmcAI AGTACT 4 cut(s) 1105, 1537, 2065, 2091
Bme1390I CCNGG 5 cut(s) 842, 1242, 1767, 1982, 2881
Bme18I GGWCC 2 cut(s) 554, 2619
BmeRI GACNNNNNGTC 1 cut(s) 3386
BmgT120I GGNCC 2 cut(s) 554, 2619
BmiI GGNNCC 2 cut(s) 765, 1403
BmrFI CCNGG 5 cut(s) 842, 1242, 1767, 1982, 2881
BmrI ACTGGG 1 cut(s) 2528
BmuI ACTGGG 1 cut(s) 2528
BpiI GAAGAC 1 cut(s) 83
Bpu10I CCTNAGC 1 cut(s) 1643
BpuEI CTTGAG 2 cut(s) 605, 1600
BsaAI YACGTR 1 cut(s) 2670
BsaHI GRCGYC 1 cut(s) 1277
BsaI GGTCTC 2 cut(s) 886, 1249
BsaJI CCNNGG 6 cut(s) 841, 998, 1169, 1765, 1980, 3315
BsaWI WCCGGW 3 cut(s) 476, 706, 2380
BsaXI ACNNNNNCTCC 2 cut(s) 1800, 1830
Bsc4I CCNNNNNNNGG 2 cut(s) 673, 1672
Bse118I RCCGGY 2 cut(s) 1258, 2380
BseAI TCCGGA 2 cut(s) 476, 706
BseBI CCWGG 5 cut(s) 842, 1242, 1767, 1982, 2881
BseDI CCNNGG 6 cut(s) 841, 998, 1169, 1765, 1980, 3315
BseLI CCNNNNNNNGG 2 cut(s) 673, 1672
BseMII CTCAG 6 cut(s) 897, 967, 1359, 1657, 3023, 3285
BseRI GAGGAG 2 cut(s) 1821, 1875
BseXI GCAGC 4 cut(s) 1017, 1488, 1904, 3297
BsgI GTGCAG 1 cut(s) 198
Bsh1236I CGCG 2 cut(s) 365, 3152
Bsh1285I CGRYCG 2 cut(s) 2098, 2702
BshFI GGCC 5 cut(s) 2299, 2452, 2461, 3161, 3301
BshTI ACCGGT 1 cut(s) 2380
BsiEI CGRYCG 2 cut(s) 2098, 2702
BsiHKAI GWGCWC 1 cut(s) 961
BsiSI CCGG 4 cut(s) 477, 707, 1259, 2381
BslFI GGGAC 2 cut(s) 395, 2114
BslI CCNNNNNNNGG 2 cut(s) 673, 1672
BsmAI GTCTC 9 cut(s) 478, 886, 1019, 1249, 1364, 1614, 2496, 2951, 3316
BsmBI CGTCTC 2 cut(s) 1019, 1364
BsmFI GGGAC 2 cut(s) 395, 2114
BsnI GGCC 5 cut(s) 2299, 2452, 2461, 3161, 3301
Bso31I GGTCTC 2 cut(s) 886, 1249
Bsp1286I GDGCHC 3 cut(s) 961, 1644, 1941
Bsp13I TCCGGA 2 cut(s) 476, 706
Bsp1407I TGTACA 1 cut(s) 1519
BspACI CCGC 8 cut(s) 204, 581, 923, 1065, 1511, 2811, 3158, 3443
BspANI GGCC 5 cut(s) 2299, 2452, 2461, 3161, 3301
BspCNI CTCAG 6 cut(s) 896, 966, 1360, 1656, 3022, 3286
BspEI TCCGGA 2 cut(s) 476, 706
BspFNI CGCG 2 cut(s) 365, 3152
BspHI TCATGA 1 cut(s) 547
BspLI GGNNCC 2 cut(s) 765, 1403
BspMAI CTGCAG 1 cut(s) 2923
BspMI ACCTGC 1 cut(s) 2926
BspPI GGATC 6 cut(s) 294, 361, 667, 925, 1102, 2871
BspQI GCTCTTC 2 cut(s) 1929, 2997
BspTI CTTAAG 1 cut(s) 3163
BspTNI GGTCTC 2 cut(s) 886, 1249
BsrFI RCCGGY 2 cut(s) 1258, 2380
BsrGI TGTACA 1 cut(s) 1519
BssAI RCCGGY 2 cut(s) 1258, 2380
BssECI CCNNGG 6 cut(s) 841, 998, 1169, 1765, 1980, 3315
BssNI GRCGYC 1 cut(s) 1277
BssSI CACGAG 3 cut(s) 426, 1515, 2631
BssT1I CCWWGG 2 cut(s) 998, 3315
Bst2BI CACGAG 3 cut(s) 426, 1515, 2631
Bst2UI CCWGG 5 cut(s) 842, 1242, 1767, 1982, 2881
Bst6I CTCTTC 6 cut(s) 1201, 1929, 2629, 2997, 3090, 3481
BstACI GRCGYC 1 cut(s) 1277
BstAFI CTTAAG 1 cut(s) 3163
BstAPI GCANNNNNTGC 3 cut(s) 44, 1898, 2918
BstAUI TGTACA 1 cut(s) 1519
BstBAI YACGTR 1 cut(s) 2670
BstC8I GCNNGC 3 cut(s) 140, 1247, 2910
BstDSI CCRYGG 1 cut(s) 1169
BstFNI CGCG 2 cut(s) 365, 3152
BstHHI GCGC 2 cut(s) 1005, 3152
BstMAI GTCTC 9 cut(s) 478, 886, 1019, 1249, 1364, 1614, 2496, 2951, 3316
BstMCI CGRYCG 2 cut(s) 2098, 2702
BstNI CCWGG 5 cut(s) 842, 1242, 1767, 1982, 2881
BstNSI RCATGY 1 cut(s) 1249
BstSCI CCNGG 5 cut(s) 840, 1240, 1765, 1980, 2879
BstSFI CTRYAG 5 cut(s) 23, 1059, 1926, 2085, 2919
BstUI CGCG 2 cut(s) 365, 3152
BstV1I GCAGC 4 cut(s) 1017, 1488, 1904, 3297
BstV2I GAAGAC 1 cut(s) 83
BstX2I RGATCY 7 cut(s) 299, 353, 659, 917, 1094, 2876, 3423
BstYI RGATCY 7 cut(s) 299, 353, 659, 917, 1094, 2876, 3423
BsuRI GGCC 5 cut(s) 2299, 2452, 2461, 3161, 3301
BtgI CCRYGG 1 cut(s) 1169
BtsI GCAGTG 2 cut(s) 186, 3348
BtsIMutI CAGTG 6 cut(s) 186, 961, 1317, 1903, 2948, 3348
BveI ACCTGC 1 cut(s) 2926
Cac8I GCNNGC 3 cut(s) 140, 1247, 2910
CaiI CAGNNNCTG 2 cut(s) 608, 1316
CciI TCATGA 1 cut(s) 547
CfoI GCGC 2 cut(s) 1005, 3152
Cfr10I RCCGGY 2 cut(s) 1258, 2380
Cfr13I GGNCC 2 cut(s) 554, 2619
CseI GACGC 4 cut(s) 371, 1285, 2549, 2669
CspAI ACCGGT 1 cut(s) 2380
DriI GACNNNNNGTC 1 cut(s) 3386
Eam1104I CTCTTC 6 cut(s) 1201, 1929, 2629, 2997, 3090, 3481
Eam1105I GACNNNNNGTC 1 cut(s) 3386
EarI CTCTTC 6 cut(s) 1201, 1929, 2629, 2997, 3090, 3481
EciI GGCGGA 1 cut(s) 1080
Eco130I CCWWGG 2 cut(s) 998, 3315
Eco147I AGGCCT 2 cut(s) 2461, 3301
Eco24I GRGCYC 2 cut(s) 1644, 1941
Eco31I GGTCTC 2 cut(s) 886, 1249
Eco32I GATATC 4 cut(s) 1325, 1681, 2986, 3015
Eco47I GGWCC 2 cut(s) 554, 2619
Eco57I CTGAAG 4 cut(s) 228, 1902, 2142, 2448
EcoO109I RGGNCCY 2 cut(s) 554, 2619
EcoRII CCWGG 5 cut(s) 840, 1240, 1765, 1980, 2879
EcoRV GATATC 4 cut(s) 1325, 1681, 2986, 3015
EcoT14I CCWWGG 2 cut(s) 998, 3315
EcoT22I ATGCAT 3 cut(s) 404, 824, 2866
EcoT38I GRGCYC 2 cut(s) 1644, 1941
ErhI CCWWGG 2 cut(s) 998, 3315
Esp3I CGTCTC 2 cut(s) 1019, 1364
FalI AAGNNNNNCTT 2 cut(s) 108, 140
FaqI GGGAC 2 cut(s) 395, 2114
FauI CCCGC 2 cut(s) 588, 2818
FauNDI CATATG 1 cut(s) 1408
FbaI TGATCA 4 cut(s) 967, 2002, 2929, 3201
Fnu4HI GCNGC 5 cut(s) 1006, 1502, 1893, 3159, 3286
FriOI GRGCYC 2 cut(s) 1644, 1941
Fsp4HI GCNGC 5 cut(s) 1006, 1502, 1893, 3159, 3286
GlaI GCGC 2 cut(s) 1004, 3151
GluI GCNGC 5 cut(s) 1006, 1502, 1893, 3159, 3286
HaeIII GGCC 5 cut(s) 2299, 2452, 2461, 3161, 3301
HapII CCGG 4 cut(s) 477, 707, 1259, 2381
HgaI GACGC 4 cut(s) 371, 1285, 2549, 2669
HhaI GCGC 2 cut(s) 1005, 3152
Hin1I GRCGYC 1 cut(s) 1277
Hin6I GCGC 2 cut(s) 1003, 3150
HinP1I GCGC 2 cut(s) 1003, 3150
HincII GTYRAC 3 cut(s) 804, 2029, 3099
HindII GTYRAC 3 cut(s) 804, 2029, 3099
HindIII AAGCTT 3 cut(s) 524, 1458, 3332
HpaII CCGG 4 cut(s) 477, 707, 1259, 2381
Hpy166II GTNNAC 6 cut(s) 167, 450, 804, 1520, 2029, 3099
Hpy8I GTNNAC 6 cut(s) 167, 450, 804, 1520, 2029, 3099
Hpy99I CGWCG 3 cut(s) 1421, 2055, 3384
HpyAV CCTTC 9 cut(s) 388, 545, 824, 985, 1789, 1821, 2289, 2472, 2992
HpyCH4IV ACGT 5 cut(s) 410, 644, 2031, 2669, 3133
HpySE526I ACGT 5 cut(s) 410, 644, 2031, 2669, 3133
Hsp92I GRCGYC 1 cut(s) 1277
HspAI GCGC 2 cut(s) 1003, 3150
Kpn2I TCCGGA 2 cut(s) 476, 706
Ksp22I TGATCA 4 cut(s) 967, 2002, 2929, 3201
LguI GCTCTTC 2 cut(s) 1929, 2997
LmnI GCTCC 3 cut(s) 227, 843, 1580
Lsp1109I GCAGC 4 cut(s) 1017, 1488, 1904, 3297
MaeII ACGT 5 cut(s) 410, 644, 2031, 2669, 3133
MfeI CAATTG 1 cut(s) 304
MflI RGATCY 7 cut(s) 299, 353, 659, 917, 1094, 2876, 3423
MhlI GDGCHC 3 cut(s) 961, 1644, 1941
MlyI GAGTC 6 cut(s) 166, 194, 1049, 1187, 1619, 2498
MmeI TCCRAC 2 cut(s) 1300, 2921
Mph1103I ATGCAT 3 cut(s) 404, 824, 2866
MroI TCCGGA 2 cut(s) 476, 706
MroXI GAANNNNTTC 1 cut(s) 1716
MslI CAYNNNNRTG 8 cut(s) 174, 426, 708, 783, 2108, 2237, 2293, 2390
MspA1I CMGCKG 2 cut(s) 206, 2858
MspCI CTTAAG 1 cut(s) 3163
MspI CCGG 4 cut(s) 477, 707, 1259, 2381
MspR9I CCNGG 5 cut(s) 842, 1242, 1767, 1982, 2881
MunI CAATTG 1 cut(s) 304
MvaI CCWGG 5 cut(s) 842, 1242, 1767, 1982, 2881
MvnI CGCG 2 cut(s) 365, 3152
NdeI CATATG 1 cut(s) 1408
NlaIV GGNNCC 2 cut(s) 765, 1403
NmuCI GTSAC 6 cut(s) 359, 812, 1722, 2851, 2935, 3035
NsiI ATGCAT 3 cut(s) 404, 824, 2866
NspI RCATGY 1 cut(s) 1249
PaeI GCATGC 1 cut(s) 1249
PagI TCATGA 1 cut(s) 547
PaqCI CACCTGC 1 cut(s) 2926
PceI AGGCCT 2 cut(s) 2461, 3301
PciSI GCTCTTC 2 cut(s) 1929, 2997
PdmI GAANNNNTTC 1 cut(s) 1716
PflFI GACNNNGTC 1 cut(s) 1834
PinAI ACCGGT 1 cut(s) 2380
PkrI GCNGC 5 cut(s) 1007, 1503, 1894, 3160, 3287
Ple19I CGATCG 1 cut(s) 2702
PleI GAGTC 6 cut(s) 165, 194, 1049, 1187, 1618, 2497
PpsI GAGTC 6 cut(s) 165, 194, 1049, 1187, 1618, 2497
Ppu21I YACGTR 1 cut(s) 2670
PpuMI RGGWCCY 2 cut(s) 554, 2619
PsiI TTATAA 2 cut(s) 18, 95
Psp1406I AACGTT 1 cut(s) 644
Psp5II RGGWCCY 2 cut(s) 554, 2619
Psp6I CCWGG 5 cut(s) 840, 1240, 1765, 1980, 2879
PspGI CCWGG 5 cut(s) 840, 1240, 1765, 1980, 2879
PspN4I GGNNCC 2 cut(s) 765, 1403
PspPI GGNCC 2 cut(s) 554, 2619
PspPPI RGGWCCY 2 cut(s) 554, 2619
PsrI GAACNNNNNNTAC 2 cut(s) 2047, 2079
PstI CTGCAG 1 cut(s) 2923
PstNI CAGNNNCTG 2 cut(s) 608, 1316
PsuI RGATCY 7 cut(s) 299, 353, 659, 917, 1094, 2876, 3423
PsyI GACNNNGTC 1 cut(s) 1834
PvuI CGATCG 1 cut(s) 2702
PvuII CAGCTG 1 cut(s) 2858
RseI CAYNNNNRTG 8 cut(s) 174, 426, 708, 783, 2108, 2237, 2293, 2390
SapI GCTCTTC 2 cut(s) 1929, 2997
SatI GCNGC 5 cut(s) 1006, 1502, 1893, 3159, 3286
Sau96I GGNCC 2 cut(s) 554, 2619
ScaI AGTACT 4 cut(s) 1105, 1537, 2065, 2091
SchI GAGTC 6 cut(s) 166, 194, 1049, 1187, 1619, 2498
ScrFI CCNGG 5 cut(s) 842, 1242, 1767, 1982, 2881
SduI GDGCHC 3 cut(s) 961, 1644, 1941
SfcI CTRYAG 5 cut(s) 23, 1059, 1926, 2085, 2919
SfiI GGCCNNNNNGGCC 1 cut(s) 2458
SinI GGWCC 2 cut(s) 554, 2619
SmiMI CAYNNNNRTG 8 cut(s) 174, 426, 708, 783, 2108, 2237, 2293, 2390
SmlI CTYRAG 3 cut(s) 584, 1615, 3163
SmoI CTYRAG 3 cut(s) 584, 1615, 3163
SpeI ACTAGT 1 cut(s) 173
SphI GCATGC 1 cut(s) 1249
SseBI AGGCCT 2 cut(s) 2461, 3301
SsiI CCGC 8 cut(s) 204, 581, 923, 1065, 1511, 2811, 3158, 3443
SspI AATATT 1 cut(s) 3208
StuI AGGCCT 2 cut(s) 2461, 3301
StyD4I CCNGG 5 cut(s) 840, 1240, 1765, 1980, 2879
StyI CCWWGG 2 cut(s) 998, 3315
TaiI ACGT 5 cut(s) 413, 647, 2034, 2672, 3136
TaqI TCGA 5 cut(s) 280, 978, 1053, 1683, 2018
TaqII GACCGA 1 cut(s) 2084
TatI WGTACW 6 cut(s) 1103, 1519, 1535, 1542, 2063, 2089
TauI GCSGC 1 cut(s) 3161
TscAI CASTG 6 cut(s) 186, 961, 1317, 1903, 2955, 3348
TseFI GTSAC 6 cut(s) 359, 812, 1722, 2851, 2935, 3035
TseI GCWGC 4 cut(s) 1005, 1501, 1892, 3285
Tsp45I GTSAC 6 cut(s) 359, 812, 1722, 2851, 2935, 3035
TspGWI ACGGA 1 cut(s) 551
TspRI CASTG 6 cut(s) 186, 961, 1317, 1903, 2955, 3348
Tth111I GACNNNGTC 1 cut(s) 1834
Vha464I CTTAAG 1 cut(s) 3163
VpaK11BI GGWCC 2 cut(s) 554, 2619
XapI RAATTY 6 cut(s) 320, 338, 536, 698, 862, 1069
XceI RCATGY 1 cut(s) 1249
XmnI GAANNNNTTC 1 cut(s) 1716
ZrmI AGTACT 4 cut(s) 1105, 1537, 2065, 2091
Zsp2I ATGCAT 3 cut(s) 404, 824, 2866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.