RchiOBHm_Chr5g0007181

May participate in a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001097
Physical Location & Seq
Reverse (-)
4503994 .. 4510117
6124 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28826

Sequence Viewer

Length: 1092 bp
ATGCGGAGTCTCACTGGTCATACAAGTCCAGTTGAATCTGTAGCTTTTAATTCAGCAGAAGTTTTGGTGCTTGCTGGAGCTTCTACTGGCGCAACAAAAATAAGGGATCTGGAGGAAACAAAGATTAAGGATCAAAGCGAGTTGTTGAAGTTTTTACGTCGGAATTCAAGCTCGCCGGATTCTGGAATTTTGCCGGCCACCGAAGCTTTCGATCGTGATATCTTGAGCTGTAGAGCTTCGTTTTGGGTGATTGACGGTCTTCCTTGGCTTTGTGTGGTTCGTATACTTACCGGACACAGATCCAGCTGTTCTGCTGTTAAATTTCATCCATTTGGTGAGTTCTTTGCTTCTGGTTCCATGGACACTAATCTGAAGATCTGGGACATTAGAAAGAAGGGATGCATCCACACATACAAAGGTCATACTCAAGGCATTAGTACTATTAAGTTCACTCCCGATGGTCGATGGGTAGTTTCTGGTGGGTTTGACAATGTTCTAAAGGTGTGGGATCTAACTGCTGGGAAGCTCTTGCATGATTTTAAATTCCATGAACGACATATTCGGTCTATAGATTTTCACCCCCTTGAGTTTCTCTTGGCCACAGGTTCAGCAGACAGAACTGTGAAATTCTGGGATTTGGAAACTTTTGAACTGATTGGATCTAGCAGGCCTGAGCCTACAAGGGGAGTACGCGCAATTACTTTCCACCCTGATGGGAGGACAATATTTTCTGGATTGGATGACAGTTTGAAGCTATTGGGATGCTCTTACTATCGAAATTCTATTGGATTCTGGGTTGCAGTCTTGCAGATGTTTGCAAGGAATCATGGAGAAGTAGAGACTGGTATAAGGAGTCCGGGTTTGCGCTGTATGTCTCCTGATTATGACACAGAGGAAATAAAGAACATATATGTTGATTCTGCAGGTGGAAAGCCAGTTACCTCACAGAGAATTAATTCTTCAAAAGTTTTACCCCCTTTGGATTTAAAGGAAACCAGTGCTAAGAAGCAGATTCCTGCAGCAGGGTCGCTCCCAAAGTCTAATGAAAAGACGGTCAATAAATCCCTAGTTGTGCCTAGCATCGTACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Katanin_con80 WD40

Protein Analysis

363

Amino Acids

40.2

Weight (kDa)

8.93

Isoelectric Point (pI)

43.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_Prp19 PF24814 3 - 171 8.4e-17 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 7 - 142 4.5e-07 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 92 - 200 1.9e-24 THOC3 beta-propeller domain
WD40_Gbeta PF25391 92 - 248 5.2e-19 G protein beta WD-40 repeat protein
WD40 PF00400 93 - 128 1.8e-09 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 93 - 249 3.4e-30 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 93 - 173 1.1e-11 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 94 - 243 6.9e-21 CDC20/Fizzy WD40 domain
WD40_MABP1-WDR62_1st PF24780 95 - 217 6.8e-10 MABP1/WDR62 first WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 95 - 161 2.1e-06 WDR90/POC16, second beta-propeller
EIF3I PF24805 95 - 193 1.8e-11 EIF3I
WD40_WDHD1_1st PF24817 95 - 140 1.4e-06 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 96 - 249 1.8e-36 WDR5 beta-propeller domain
Beta-prop_EML PF23409 97 - 218 9.7e-10 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR75_1st PF23869 98 - 172 6.4e-06 WD repeat-containing protein 75 first beta-propeller
Beta-prop_EML_2 PF23414 100 - 193 9e-11 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_Aladin PF25460 100 - 172 3e-08 Aladin seven-bladed propeller
WD40_MABP1-WDR62_2nd PF24782 105 - 171 4.1e-07 MABP1/WDR62 second WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 113 - 169 4.7e-07 WDR36/Utp21 first beta-propeller
WDR55 PF24796 113 - 176 4.3e-08 WDR55
Beta-prop_WDR36-Utp21_1st PF25171 118 - 251 3.2e-11 WDR36/Utp21 first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 128 - 201 1.5e-11 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 134 - 249 1.3e-20 Prp19 WD40 domain
WD40 PF00400 134 - 170 7e-12 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 136 - 217 2.1e-10 CAF1B/HIR1 beta-propeller domain
EIF3I PF24805 139 - 249 2.7e-12 EIF3I
WD40_WDHD1_1st PF24817 142 - 218 6.6e-10 WDHD1 first WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 163 - 245 2.7e-08 WDR90/POC16, second beta-propeller
Beta-prop_WDR3_2nd PF25172 166 - 217 8.9e-07 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 166 - 249 1.9e-12 THOC3 beta-propeller domain
WD40 PF00400 174 - 212 5.4e-08 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 914
Acc36I ACCTGC 1 cut(s) 914
AccI GTMKAC 1 cut(s) 283
AccII CGCG 1 cut(s) 693
AciI CCGC 1 cut(s) 4
AclWI GGATC 5 cut(s) 114, 138, 294, 516, 667
AcoI YGGCCR 2 cut(s) 195, 597
AcsI RAATTY 6 cut(s) 163, 186, 320, 542, 626, 778
AcuI CTGAAG 1 cut(s) 392
AfaI GTAC 3 cut(s) 439, 690, 1086
AfiI CCNNNNNNNGG 3 cut(s) 182, 683, 1022
AgsI TTSAA 6 cut(s) 35, 148, 168, 650, 751, 963
AluBI AGCT 9 cut(s) 44, 80, 171, 206, 228, 236, 306, 526, 754
AluI AGCT 9 cut(s) 44, 80, 171, 206, 228, 236, 306, 526, 754
Alw26I GTCTC 3 cut(s) 14, 833, 879
AlwI GGATC 5 cut(s) 114, 138, 294, 516, 667
AoxI GGCC 3 cut(s) 195, 597, 668
ApeKI GCWGC 1 cut(s) 1019
ApoI RAATTY 6 cut(s) 163, 186, 320, 542, 626, 778
AseI ATTAAT 1 cut(s) 954
Asp700I GAANNNNTTC 1 cut(s) 955
AspLEI GCGC 3 cut(s) 92, 695, 867
AsuC2I CCSGG 1 cut(s) 858
AsuHPI GGTGA 3 cut(s) 259, 347, 569
BalI TGGCCA 1 cut(s) 599
BbsI GAAGAC 1 cut(s) 251
BbvI GCAGC 1 cut(s) 1031
BccI CCATC 3 cut(s) 452, 459, 707
BcgI CGANNNNNNTGC 2 cut(s) 1008, 1042
BcnI CCSGG 1 cut(s) 858
BcoDI GTCTC 3 cut(s) 14, 833, 879
BfaI CTAG 3 cut(s) 663, 1067, 1077
BfmI CTRYAG 5 cut(s) 39, 229, 567, 921, 1017
BfuAI ACCTGC 1 cut(s) 914
BglII AGATCT 1 cut(s) 375
BisI GCNGC 1 cut(s) 1020
BlsI GCNGC 1 cut(s) 1021
BmcAI AGTACT 1 cut(s) 439
Bme1390I CCNGG 1 cut(s) 858
BmiI GGNNCC 1 cut(s) 355
BmrFI CCNGG 1 cut(s) 858
BmsI GCATC 3 cut(s) 389, 411, 752
BpiI GAAGAC 1 cut(s) 251
BpmI CTGGAG 2 cut(s) 96, 131
Bpu10I CCTNAGC 1 cut(s) 672
BpuEI CTTGAG 3 cut(s) 244, 411, 605
BpuMI CCSGG 1 cut(s) 858
BsaJI CCNNGG 2 cut(s) 263, 357
BsaWI WCCGGW 1 cut(s) 290
BsaXI ACNNNNNCTCC 2 cut(s) 844, 874
Bsc4I CCNNNNNNNGG 3 cut(s) 182, 683, 1022
Bse118I RCCGGY 1 cut(s) 193
Bse1I ACTGG 6 cut(s) 19, 29, 91, 847, 935, 996
BseDI CCNNGG 2 cut(s) 263, 357
BseGI GGATG 5 cut(s) 325, 402, 404, 745, 767
BseLI CCNNNNNNNGG 3 cut(s) 182, 683, 1022
BseMII CTCAG 1 cut(s) 663
BseNI ACTGG 6 cut(s) 19, 29, 91, 847, 935, 996
BseXI GCAGC 1 cut(s) 1031
BseYI CCCAGC 1 cut(s) 518
Bsh1236I CGCG 1 cut(s) 693
Bsh1285I CGRYCG 1 cut(s) 214
BshFI GGCC 3 cut(s) 197, 599, 670
BsiEI CGRYCG 1 cut(s) 214
BsiSI CCGG 4 cut(s) 176, 194, 291, 857
BslFI GGGAC 1 cut(s) 395
BslI CCNNNNNNNGG 3 cut(s) 182, 683, 1022
BsmAI GTCTC 3 cut(s) 14, 833, 879
BsmFI GGGAC 1 cut(s) 395
BsnI GGCC 3 cut(s) 197, 599, 670
Bsp143I GATC 7 cut(s) 106, 130, 211, 299, 375, 508, 659
Bsp19I CCATGG 1 cut(s) 357
BspACI CCGC 1 cut(s) 4
BspANI GGCC 3 cut(s) 197, 599, 670
BspCNI CTCAG 1 cut(s) 664
BspFNI CGCG 1 cut(s) 693
BspLI GGNNCC 1 cut(s) 355
BspMAI CTGCAG 2 cut(s) 925, 1021
BspMI ACCTGC 1 cut(s) 914
BspPI GGATC 5 cut(s) 114, 138, 294, 516, 667
BsrFI RCCGGY 1 cut(s) 193
BsrI ACTGG 6 cut(s) 19, 29, 91, 847, 935, 996
BssAI RCCGGY 1 cut(s) 193
BssECI CCNNGG 2 cut(s) 263, 357
BssMI GATC 7 cut(s) 106, 130, 211, 299, 375, 508, 659
BssNAI GTATAC 1 cut(s) 284
BssT1I CCWWGG 2 cut(s) 263, 357
Bst1107I GTATAC 1 cut(s) 284
Bst4CI ACNGT 4 cut(s) 257, 622, 746, 1054
BstC8I GCNNGC 4 cut(s) 72, 173, 195, 668
BstDEI CTNAG 2 cut(s) 672, 1002
BstDSI CCRYGG 1 cut(s) 357
BstENI CCTNNNNNAGG 1 cut(s) 1020
BstF5I GGATG 5 cut(s) 325, 402, 404, 745, 767
BstFNI CGCG 1 cut(s) 693
BstHHI GCGC 3 cut(s) 92, 695, 867
BstKTI GATC 7 cut(s) 109, 133, 214, 302, 378, 511, 662
BstMAI GTCTC 3 cut(s) 14, 833, 879
BstMBI GATC 7 cut(s) 106, 130, 211, 299, 375, 508, 659
BstMCI CGRYCG 1 cut(s) 214
BstMWI GCNNNNNNNGC 1 cut(s) 203
BstSCI CCNGG 1 cut(s) 856
BstSFI CTRYAG 5 cut(s) 39, 229, 567, 921, 1017
BstUI CGCG 1 cut(s) 693
BstV1I GCAGC 1 cut(s) 1031
BstV2I GAAGAC 1 cut(s) 251
BstX2I RGATCY 5 cut(s) 106, 299, 375, 508, 659
BstXI CCANNNNNNTGG 1 cut(s) 713
BstYI RGATCY 5 cut(s) 106, 299, 375, 508, 659
BstZ17I GTATAC 1 cut(s) 284
BsuRI GGCC 3 cut(s) 197, 599, 670
BtgI CCRYGG 1 cut(s) 357
BtsCI GGATG 5 cut(s) 325, 402, 404, 745, 767
BtsIMutI CAGTG 2 cut(s) 12, 1003
BveI ACCTGC 1 cut(s) 914
Cac8I GCNNGC 4 cut(s) 72, 173, 195, 668
CfoI GCGC 3 cut(s) 92, 695, 867
Cfr10I RCCGGY 1 cut(s) 193
Csp6I GTAC 3 cut(s) 438, 689, 1085
CviAII CATG 4 cut(s) 358, 533, 548, 827
CviQI GTAC 3 cut(s) 438, 689, 1085
DdeI CTNAG 2 cut(s) 672, 1002
DpnI GATC 7 cut(s) 108, 132, 213, 301, 377, 510, 661
DpnII GATC 7 cut(s) 106, 130, 211, 299, 375, 508, 659
DraI TTTAAA 2 cut(s) 541, 987
EaeI YGGCCR 2 cut(s) 195, 597
Eco130I CCWWGG 2 cut(s) 263, 357
Eco147I AGGCCT 1 cut(s) 670
Eco32I GATATC 1 cut(s) 220
Eco57I CTGAAG 1 cut(s) 392
EcoNI CCTNNNNNAGG 1 cut(s) 1020
EcoRI GAATTC 1 cut(s) 163
EcoRV GATATC 1 cut(s) 220
EcoT14I CCWWGG 2 cut(s) 263, 357
EcoT22I ATGCAT 1 cut(s) 404
ErhI CCWWGG 2 cut(s) 263, 357
FaeI CATG 4 cut(s) 361, 536, 551, 830
FaqI GGGAC 1 cut(s) 395
FatI CATG 4 cut(s) 357, 532, 547, 826
FblI GTMKAC 1 cut(s) 283
Fnu4HI GCNGC 1 cut(s) 1020
FokI GGATG 5 cut(s) 312, 389, 411, 752, 774
Fsp4HI GCNGC 1 cut(s) 1020
FspBI CTAG 3 cut(s) 663, 1067, 1077
GlaI GCGC 3 cut(s) 91, 694, 866
GluI GCNGC 1 cut(s) 1020
GsaI CCCAGC 1 cut(s) 522
GsuI CTGGAG 2 cut(s) 96, 131
HaeIII GGCC 3 cut(s) 197, 599, 670
HapII CCGG 4 cut(s) 176, 194, 291, 857
HhaI GCGC 3 cut(s) 92, 695, 867
Hin1II CATG 4 cut(s) 361, 536, 551, 830
Hin6I GCGC 3 cut(s) 90, 693, 865
HinP1I GCGC 3 cut(s) 90, 693, 865
HindIII AAGCTT 1 cut(s) 204
HinfI GANTC 8 cut(s) 7, 35, 179, 789, 823, 853, 917, 1012
HpaII CCGG 4 cut(s) 176, 194, 291, 857
HphI GGTGA 3 cut(s) 259, 347, 569
Hpy166II GTNNAC 2 cut(s) 284, 450
Hpy188I TCNGA 2 cut(s) 162, 372
Hpy188III TCNNGA 7 cut(s) 110, 183, 215, 223, 455, 732, 878
Hpy8I GTNNAC 2 cut(s) 284, 450
Hpy99I CGWCG 1 cut(s) 162
HpyAV CCTTC 1 cut(s) 388
HpyCH4III ACNGT 4 cut(s) 257, 622, 746, 1054
HpyCH4IV ACGT 1 cut(s) 157
HpyCH4V TGCA 7 cut(s) 402, 532, 800, 808, 818, 923, 1019
HpyF10VI GCNNNNNNNGC 1 cut(s) 203
HpyF3I CTNAG 2 cut(s) 672, 1002
HpySE526I ACGT 1 cut(s) 157
Hsp92II CATG 4 cut(s) 361, 536, 551, 830
HspAI GCGC 3 cut(s) 90, 693, 865
KroI GCCGGC 1 cut(s) 193
KroNI GCCGGC 1 cut(s) 195
Kzo9I GATC 7 cut(s) 106, 130, 211, 299, 375, 508, 659
LmnI GCTCC 2 cut(s) 77, 1035
Lsp1109I GCAGC 1 cut(s) 1031
LweI GCATC 3 cut(s) 389, 411, 752
MaeI CTAG 3 cut(s) 663, 1067, 1077
MaeII ACGT 1 cut(s) 157
MaeIII GTNAC 1 cut(s) 937
MalI GATC 7 cut(s) 108, 132, 213, 301, 377, 510, 661
MboI GATC 7 cut(s) 106, 130, 211, 299, 375, 508, 659
MboII GAAGA 3 cut(s) 251, 385, 951
MflI RGATCY 5 cut(s) 106, 299, 375, 508, 659
MlsI TGGCCA 1 cut(s) 599
MluNI TGGCCA 1 cut(s) 599
MlyI GAGTC 2 cut(s) 16, 862
MmeI TCCRAC 1 cut(s) 140
MnlI CCTC 4 cut(s) 106, 711, 886, 952
Mox20I TGGCCA 1 cut(s) 599
Mph1103I ATGCAT 1 cut(s) 404
MroNI GCCGGC 1 cut(s) 193
MroXI GAANNNNTTC 1 cut(s) 955
MscI TGGCCA 1 cut(s) 599
MseI TTAA 7 cut(s) 48, 126, 318, 444, 540, 954, 986
MslI CAYNNNNRTG 1 cut(s) 711
Msp20I TGGCCA 1 cut(s) 599
MspA1I CMGCKG 1 cut(s) 306
MspI CCGG 4 cut(s) 176, 194, 291, 857
MspR9I CCNGG 1 cut(s) 858
MvnI CGCG 1 cut(s) 693
MwoI GCNNNNNNNGC 1 cut(s) 203
NaeI GCCGGC 1 cut(s) 195
NciI CCSGG 1 cut(s) 858
NcoI CCATGG 1 cut(s) 357
NdeII GATC 7 cut(s) 106, 130, 211, 299, 375, 508, 659
NgoMIV GCCGGC 1 cut(s) 193
NlaIII CATG 4 cut(s) 361, 536, 551, 830
NlaIV GGNNCC 1 cut(s) 355
NsiI ATGCAT 1 cut(s) 404
PaqCI CACCTGC 1 cut(s) 914
PceI AGGCCT 1 cut(s) 670
PdiI GCCGGC 1 cut(s) 195
PdmI GAANNNNTTC 1 cut(s) 955
PfeI GAWTC 6 cut(s) 35, 179, 789, 823, 917, 1012
PkrI GCNGC 1 cut(s) 1021
Ple19I CGATCG 1 cut(s) 214
PleI GAGTC 2 cut(s) 15, 861
PpsI GAGTC 2 cut(s) 15, 861
PshBI ATTAAT 1 cut(s) 954
PspFI CCCAGC 1 cut(s) 518
PspN4I GGNNCC 1 cut(s) 355
PstI CTGCAG 2 cut(s) 925, 1021
PsuI RGATCY 5 cut(s) 106, 299, 375, 508, 659
PvuI CGATCG 1 cut(s) 214
PvuII CAGCTG 1 cut(s) 306
RsaI GTAC 3 cut(s) 439, 690, 1086
RsaNI GTAC 3 cut(s) 438, 689, 1085
RseI CAYNNNNRTG 1 cut(s) 711
SaqAI TTAA 7 cut(s) 48, 126, 318, 444, 540, 954, 986
SatI GCNGC 1 cut(s) 1020
Sau3AI GATC 7 cut(s) 106, 130, 211, 299, 375, 508, 659
ScaI AGTACT 1 cut(s) 439
SchI GAGTC 2 cut(s) 16, 862
ScrFI CCNGG 1 cut(s) 858
SfaNI GCATC 3 cut(s) 389, 411, 752
SfcI CTRYAG 5 cut(s) 39, 229, 567, 921, 1017
SmiMI CAYNNNNRTG 1 cut(s) 711
SmlI CTYRAG 3 cut(s) 223, 426, 584
SmoI CTYRAG 3 cut(s) 223, 426, 584
SseBI AGGCCT 1 cut(s) 670
SsiI CCGC 1 cut(s) 4
SspI AATATT 1 cut(s) 726
SspMI CTAG 3 cut(s) 663, 1067, 1077
StuI AGGCCT 1 cut(s) 670
StyD4I CCNGG 1 cut(s) 856
StyI CCWWGG 2 cut(s) 263, 357
TaaI ACNGT 4 cut(s) 257, 622, 746, 1054
TaiI ACGT 1 cut(s) 160
TaqI TCGA 3 cut(s) 210, 463, 775
TaqII GACCGA 1 cut(s) 552
TatI WGTACW 1 cut(s) 437
TfiI GAWTC 6 cut(s) 35, 179, 789, 823, 917, 1012
Tru1I TTAA 7 cut(s) 48, 126, 318, 444, 540, 954, 986
Tru9I TTAA 7 cut(s) 48, 126, 318, 444, 540, 954, 986
TscAI CASTG 2 cut(s) 19, 1003
TseI GCWGC 1 cut(s) 1019
TspDTI ATGAA 3 cut(s) 314, 564, 1059
TspRI CASTG 2 cut(s) 19, 1003
VspI ATTAAT 1 cut(s) 954
XagI CCTNNNNNAGG 1 cut(s) 1020
XapI RAATTY 6 cut(s) 163, 186, 320, 542, 626, 778
XmiI GTMKAC 1 cut(s) 283
XmnI GAANNNNTTC 1 cut(s) 955
XspI CTAG 3 cut(s) 663, 1067, 1077
ZrmI AGTACT 1 cut(s) 439
Zsp2I ATGCAT 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.