FvH4_3g04490

May participate in a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001097
Physical Location & Seq
Reverse (-)
2565092 .. 2573380
8289 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g04490.t3

Sequence Viewer

Length: 2166 bp
ATGGGATCTGGAGGAAGCGAAGATGTAGTGGTTCGCACACTTACTGGACACAGATCCAGCTGTTCTGCTGTTGAATTTCATCCATTTGGTGAGTTCTTTGCTTCTGGTTCCACGGACACTAATCTAAAGATCTGGGACATTAGAAAGAAGGGATGCATCCACACATACAAAGGTCATACTCAAGGCATTAGTACTATTAAGTTCACTCCCGATGGTCGATGGGTTGTCTCTGGTGGGTTTGATAATGTTGTAAAGGTGTGGGATCTAACTGCTGGGAAGCTCTTGCATGATTTTAAATTCCATGAAGGACATATTCGGTCTATAGATTTTCATCCCCTTGAGTTTCTCTTGGCCACAGGTTCAGCAGACAGAACCGTGAAATTCTGGGATTTGGAAACTTTTGAATTGATTGGATCTACCAGGCCTGAGCCTACAGGAGTACGCTCAATTGCTTTCCACCCTGATGGGAGGACGATATTTTCTGGATTGGACGACAGATTGAAGGTTTATTCCTGGGAGCCTGTAATATCTCATGATTCTGTTGAAATGGGATGGTCAAGACTTGCTGACCTATGCATTCATGATGGGAAGCTACTGGGATGCTCTTACTATCGAAATTCTATTGGAGTCTGGGTTGCTGACGTTTCGCTTATTAAGCCATATGGAGATGGTTCAACACCTGAGCAAAATGACTGTTTCGATGAGAAATCTCATGTAAAGTTGCACGGAATCATGGAGAATGTAGAGACTGGTATAAGGGGTCCGGGGTTGCGCTGTATGTCTCCTGATTATGACTCAAAGGACATAAAGAACATATATGTTGATTCTGCATGTGGAAAGCCAGTTACCACACAGAGAATTAATTCTTCGAAAGTTTTACCCCCATTGGAATTAAAGGAAACTGGTGCTAAGAAGCAGATTCCTGCAGCGGGGTCACTCCCAAAGTCTAATGAAAAGATGGTCAATAAATCCTTGGCTGTGCCTAGCATTGTACCTCGAAGCAGTCCCAATGCAAAAGACTCGGCTAACACTGGAAGAGAATCTGTTGCATTTTCAAAAACTAGGCGTGGTATGTTGCTCAGACCAGCTCATGTGCGAAGCCCATCAGATAGCAAAGTTGACATGGAGAAACTACCGTTCGCCGTTGAATCCGGAACTTTCAGCAGCATGACGAGTAAATTGGATGGTGCAGTGGGTCTCAGTTCTCAGATCAAAACTATAGCAGAAGATAGTGATAGAGAGTCCTGTAAGGAAAAGCATTCTGTAATCAAGAGTGTAGAAGAGAAATTTGAAGCAGTATTATCACTCAAGAGGCCCTCTGATCAGGAAACTAAAAATGGACTCCTCAGTAACAGCAAGGAATTGAGCCCTGTTAAAGTTGTCAATGGAGTTGCTGTTGTACCAGGAAGGACTCGCACCCTGGTTGAGCGATTTGAAAGGAGAGAGAGATTTAGTAGCAATGATGATCAAGCAACTGGCATAATTGGTCCTGTGATACCTGAAGATCAAGCAAGTAGCATATCTAGTCCTGCGATCCCTGAAGATCAAGCAACTAGCATATGTAGTCCCATGACAATTGAAATGGAGAAAACATTCACCGTGCAGCGAGAAGAGGAACCTCAAATTTCTGGGAGGCACATGTCATCTGCGAAAAATGATCCTACTGAAGATCTGATGCAAACTCATGATGCATTCCTAAGTACCCTTCGATCCCGCCTGACAAAACTGCAGGTGGTGAGGCATTTTTGGGAACGGAATGATATTAAAGGTGCTGTCACTGCTATCAGAAAGCTTCCAGATCATGCGGTACAAGCGGATGTGATTAGTGTTCTCCTGGATAAACTGGATATTTTGACATTAGATCTCTTTTCGTGTTTGCTGCCTGTGCTTCTGGGCTTGCTTGATAGCAAAATTGAGAGGCATGCAAATGTGTCATTGGAGATGTTGTTGAAGCTTGTAGCAGTCTTTGGCCCAGTGATACAGTCTACCATTTCAGCACCTCCAGCGGTCGGAGTCAATCTGCAGGCAGAGCAAAGGCTGGAGTGCTGCAACCAATGCTCTATATACCTGCAAAAGATTCAGAAAATTCTCCCAGTCTTTGTAAGGAAAGGGGGTGTGCTGGCAAGATGTGCTAGAGAATTAAATCTAATTCTTCAACAGTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Katanin_con80 WD40

Protein Analysis

722

Amino Acids

79.46

Weight (kDa)

7.29

Isoelectric Point (pI)

43.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 8 - 134 6.7e-25 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 8 - 129 8e-10 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 9 - 129 1.3e-20 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 10 - 132 6.4e-14 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 12 - 136 2.2e-10 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 13 - 127 2.1e-07 WDR36/Utp21 first beta-propeller
WD40_MABP1-WDR62_2nd PF24782 14 - 171 3.2e-10 MABP1/WDR62 second WD40 domain
WD40_Prp19 PF24814 15 - 254 9.1e-46 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 20 - 140 2.5e-16 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 29 - 177 1.3e-17 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 51 - 173 3.6e-18 WDR3 second beta-propeller domain
EIF3I PF24805 52 - 138 4e-07 EIF3I
Beta-prop_CAF1B_HIR1 PF24105 52 - 137 6.6e-07 CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz PF24807 53 - 245 3e-31 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 56 - 201 1.5e-20 WDR36/Utp21 second beta-propeller domain
Beta-prop_SPT8 PF23798 57 - 152 8.2e-06 SPT8 beta propeller
Beta-prop_THOC3 PF25174 59 - 194 5.6e-30 THOC3 beta-propeller domain
WDR55 PF24796 73 - 256 6.8e-16 WDR55
Beta-prop_WDR3_1st PF25173 79 - 253 1e-37 WDR3 first beta-propeller domain
WD40_MABP1-WDR62_1st PF24780 84 - 219 6.2e-10 MABP1/WDR62 first WD40 domain
WD40 PF00400 91 - 128 5e-09 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 95 - 249 1.2e-36 WDR5 beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 98 - 161 6.2e-06 WDR90/POC16, second beta-propeller
Beta-prop_Aladin PF25460 101 - 194 6.3e-08 Aladin seven-bladed propeller
Beta-prop_TEP1_2nd PF25047 115 - 187 2.6e-06 TEP-1 second beta-propeller
Beta-prop_SPT8 PF23798 117 - 203 5.4e-06 SPT8 beta propeller
Beta-prop_WDR36-Utp21_1st PF25171 118 - 252 1.4e-11 WDR36/Utp21 first beta-propeller
WD40_Gbeta PF25391 119 - 255 7.5e-17 G protein beta WD-40 repeat protein
WD40 PF00400 134 - 170 7.9e-12 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 136 - 219 1.6e-10 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 136 - 254 7.1e-06 TEP-1 second beta-propeller
Beta-prop_EML PF23409 138 - 241 7.3e-07 Echinoderm microtubule-associated protein first beta-propeller
EIF3I PF24805 139 - 251 9.4e-15 EIF3I
WD40_WDHD1_1st PF24817 143 - 220 2.5e-10 WDHD1 first WD40 domain
Beta-prop_WDR3_2nd PF25172 163 - 248 6.1e-08 WDR3 second beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 163 - 244 1.6e-09 WDR90/POC16, second beta-propeller
Beta-prop_THOC3 PF25174 166 - 248 2.1e-13 THOC3 beta-propeller domain
WD40 PF00400 174 - 212 5.4e-08 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 189 - 247 9.2e-07 Echinoderm microtubule-associated protein second beta-propeller
Katanin_con80 PF13925 642 - 799 1.4e-52 con80 domain of Katanin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1720
Acc36I ACCTGC 2 cut(s) 1720, 2076
AccI GTMKAC 1 cut(s) 1985
AccIII TCCGGA 1 cut(s) 1151
AciI CCGC 5 cut(s) 929, 1714, 1805, 1814, 2006
AclWI GGATC 7 cut(s) 13, 48, 270, 421, 1528, 1652, 1704
AcoI YGGCCR 1 cut(s) 351
AcsI RAATTY 7 cut(s) 74, 296, 380, 616, 1286, 1623, 2085
AcuI CTGAAG 3 cut(s) 1521, 1560, 1686
AfaI GTAC 6 cut(s) 193, 441, 993, 1401, 1702, 1809
AfiI CCNNNNNNNGG 2 cut(s) 929, 2009
AflIII ACRYGT 1 cut(s) 1638
AjnI CCWGG 5 cut(s) 419, 512, 1402, 1419, 1833
AluBI AGCT 6 cut(s) 60, 280, 592, 1088, 1792, 1954
AluI AGCT 6 cut(s) 60, 280, 592, 1088, 1792, 1954
Alw26I GTCTC 4 cut(s) 232, 740, 786, 1202
AlwI GGATC 7 cut(s) 13, 48, 270, 421, 1528, 1652, 1704
Aor13HI TCCGGA 1 cut(s) 1151
AoxI GGCC 4 cut(s) 351, 422, 1313, 1969
ApeKI GCWGC 5 cut(s) 926, 1164, 1603, 1879, 2046
ApoI RAATTY 7 cut(s) 74, 296, 380, 616, 1286, 1623, 2085
AseI ATTAAT 1 cut(s) 861
Asp700I GAANNNNTTC 2 cut(s) 862, 1592
AspLEI GCGC 1 cut(s) 774
AspS9I GGNCC 4 cut(s) 761, 1314, 1487, 1970
AsuC2I CCSGG 1 cut(s) 765
AsuHPI GGTGA 3 cut(s) 101, 1588, 1747
AsuII TTCGAA 1 cut(s) 869
AvaII GGWCC 2 cut(s) 761, 1487
BalI TGGCCA 1 cut(s) 353
BanII GRGCYC 1 cut(s) 1370
BbvI GCAGC 5 cut(s) 938, 1176, 1615, 1866, 2033
BccI CCATC 9 cut(s) 206, 213, 458, 546, 578, 662, 952, 1111, 1178
BceAI ACGGC 1 cut(s) 1127
BcgI CGANNNNNNTGC 2 cut(s) 1002, 1036
BciT130I CCWGG 5 cut(s) 421, 514, 1404, 1421, 1835
BclI TGATCA 2 cut(s) 1321, 1465
BcnI CCSGG 1 cut(s) 765
BcoDI GTCTC 4 cut(s) 232, 740, 786, 1202
BfaI CTAG 5 cut(s) 984, 1062, 1524, 1554, 2133
BfmI CTRYAG 6 cut(s) 321, 432, 924, 1218, 1727, 2021
BfuAI ACCTGC 2 cut(s) 1720, 2076
BglII AGATCT 3 cut(s) 129, 1669, 1861
BisI GCNGC 5 cut(s) 927, 1165, 1604, 1880, 2047
BlsI GCNGC 5 cut(s) 928, 1166, 1605, 1881, 2048
BmcAI AGTACT 1 cut(s) 193
Bme1390I CCNGG 6 cut(s) 421, 514, 765, 1404, 1421, 1835
Bme18I GGWCC 2 cut(s) 761, 1487
BmgT120I GGNCC 4 cut(s) 761, 1314, 1487, 1970
BmiI GGNNCC 4 cut(s) 109, 519, 762, 1617
BmrFI CCNGG 6 cut(s) 421, 514, 765, 1404, 1421, 1835
BmrI ACTGGG 3 cut(s) 605, 1967, 2087
BmsI GCATC 5 cut(s) 143, 165, 590, 1665, 1678
BmuI ACTGGG 3 cut(s) 605, 1967, 2087
BpmI CTGGAG 3 cut(s) 30, 1986, 2060
Bpu10I CCTNAGC 2 cut(s) 426, 681
Bpu14I TTCGAA 1 cut(s) 869
BpuEI CTTGAG 3 cut(s) 165, 359, 1292
BpuMI CCSGG 1 cut(s) 765
BsaBI GATNNNNATC 1 cut(s) 330
BsaI GGTCTC 1 cut(s) 1202
BsaJI CCNNGG 5 cut(s) 111, 513, 764, 972, 1419
BsaWI WCCGGW 1 cut(s) 1151
BsaXI ACNNNNNCTCC 6 cut(s) 618, 648, 1380, 1410, 1931, 1961
Bsc4I CCNNNNNNNGG 2 cut(s) 929, 2009
Bse3DI GCAATG 1 cut(s) 1465
Bse8I GATNNNNATC 1 cut(s) 330
BseAI TCCGGA 1 cut(s) 1151
BseBI CCWGG 5 cut(s) 421, 514, 1404, 1421, 1835
BseDI CCNNGG 5 cut(s) 111, 513, 764, 972, 1419
BseGI GGATG 8 cut(s) 79, 156, 158, 331, 557, 605, 1189, 1822
BseJI GATNNNNATC 1 cut(s) 330
BseLI CCNNNNNNNGG 2 cut(s) 929, 2009
BseMI GCAATG 1 cut(s) 1465
BseMII CTCAG 6 cut(s) 417, 672, 1093, 1213, 1220, 1360
BseRI GAGGAG 1 cut(s) 1334
BseXI GCAGC 5 cut(s) 938, 1176, 1615, 1866, 2033
BseYI CCCAGC 1 cut(s) 272
BsgI GTGCAG 2 cut(s) 1209, 1622
Bsh1285I CGRYCG 1 cut(s) 2010
BshFI GGCC 4 cut(s) 353, 424, 1315, 1971
BsiEI CGRYCG 1 cut(s) 2010
BsiSI CCGG 2 cut(s) 764, 1152
BslFI GGGAC 3 cut(s) 149, 990, 1551
BslI CCNNNNNNNGG 2 cut(s) 929, 2009
BsmAI GTCTC 4 cut(s) 232, 740, 786, 1202
BsmFI GGGAC 3 cut(s) 149, 990, 1551
BsmI GAATGC 3 cut(s) 576, 1258, 1691
BsnI GGCC 4 cut(s) 353, 424, 1315, 1971
Bso31I GGTCTC 1 cut(s) 1202
Bsp119I TTCGAA 1 cut(s) 869
Bsp1286I GDGCHC 1 cut(s) 1370
Bsp13I TCCGGA 1 cut(s) 1151
BspACI CCGC 5 cut(s) 929, 1714, 1805, 1814, 2006
BspANI GGCC 4 cut(s) 353, 424, 1315, 1971
BspCNI CTCAG 6 cut(s) 418, 673, 1092, 1212, 1219, 1359
BspEI TCCGGA 1 cut(s) 1151
BspHI TCATGA 3 cut(s) 532, 580, 1684
BspLI GGNNCC 4 cut(s) 109, 519, 762, 1617
BspMAI CTGCAG 3 cut(s) 928, 1731, 2025
BspMI ACCTGC 2 cut(s) 1720, 2076
BspPI GGATC 7 cut(s) 13, 48, 270, 421, 1528, 1652, 1704
BspT104I TTCGAA 1 cut(s) 869
BspTNI GGTCTC 1 cut(s) 1202
BsrDI GCAATG 1 cut(s) 1465
BssECI CCNNGG 5 cut(s) 111, 513, 764, 972, 1419
BssT1I CCWWGG 1 cut(s) 972
Bst2UI CCWGG 5 cut(s) 421, 514, 1404, 1421, 1835
Bst4CI ACNGT 6 cut(s) 376, 695, 1137, 1600, 1983, 2160
Bst6I CTCTTC 3 cut(s) 1030, 1275, 1605
BstAPI GCANNNNNTGC 2 cut(s) 2055, 2129
BstBI TTCGAA 1 cut(s) 869
BstC8I GCNNGC 4 cut(s) 1898, 1923, 2025, 2121
BstDEI CTNAG 8 cut(s) 426, 681, 909, 1079, 1199, 1206, 1346, 1697
BstDSI CCRYGG 1 cut(s) 111
BstF5I GGATG 8 cut(s) 79, 156, 158, 331, 557, 605, 1189, 1822
BstHHI GCGC 1 cut(s) 774
BstMAI GTCTC 4 cut(s) 232, 740, 786, 1202
BstMCI CGRYCG 1 cut(s) 2010
BstMWI GCNNNNNNNGC 8 cut(s) 655, 1778, 1811, 1885, 2003, 2029, 2055, 2129
BstNI CCWGG 5 cut(s) 421, 514, 1404, 1421, 1835
BstNSI RCATGY 3 cut(s) 834, 1642, 1925
BstSCI CCNGG 6 cut(s) 419, 512, 763, 1402, 1419, 1833
BstSFI CTRYAG 6 cut(s) 321, 432, 924, 1218, 1727, 2021
BstV1I GCAGC 5 cut(s) 938, 1176, 1615, 1866, 2033
BstX2I RGATCY 7 cut(s) 5, 53, 129, 262, 413, 1669, 1861
BstXI CCANNNNNNTGG 1 cut(s) 464
BstYI RGATCY 7 cut(s) 5, 53, 129, 262, 413, 1669, 1861
BsuRI GGCC 4 cut(s) 353, 424, 1315, 1971
BtgI CCRYGG 1 cut(s) 111
BtsCI GGATG 8 cut(s) 79, 156, 158, 331, 557, 605, 1189, 1822
BtsI GCAGTG 2 cut(s) 1197, 1776
BtsIMutI CAGTG 4 cut(s) 1029, 1197, 1776, 1980
BveI ACCTGC 2 cut(s) 1720, 2076
Cac8I GCNNGC 4 cut(s) 1898, 1923, 2025, 2121
CciI TCATGA 3 cut(s) 532, 580, 1684
CfoI GCGC 1 cut(s) 774
Cfr13I GGNCC 4 cut(s) 761, 1314, 1487, 1970
Csp6I GTAC 6 cut(s) 192, 440, 992, 1400, 1701, 1808
CviQI GTAC 6 cut(s) 192, 440, 992, 1400, 1701, 1808
DdeI CTNAG 8 cut(s) 426, 681, 909, 1079, 1199, 1206, 1346, 1697
DraI TTTAAA 1 cut(s) 295
EaeI YGGCCR 1 cut(s) 351
Eam1104I CTCTTC 3 cut(s) 1030, 1275, 1605
EarI CTCTTC 3 cut(s) 1030, 1275, 1605
Eco130I CCWWGG 1 cut(s) 972
Eco147I AGGCCT 1 cut(s) 424
Eco24I GRGCYC 1 cut(s) 1370
Eco31I GGTCTC 1 cut(s) 1202
Eco47I GGWCC 2 cut(s) 761, 1487
Eco57I CTGAAG 3 cut(s) 1521, 1560, 1686
EcoO109I RGGNCCY 1 cut(s) 1314
EcoRII CCWGG 5 cut(s) 419, 512, 1402, 1419, 1833
EcoT14I CCWWGG 1 cut(s) 972
EcoT22I ATGCAT 3 cut(s) 158, 578, 1693
EcoT38I GRGCYC 1 cut(s) 1370
ErhI CCWWGG 1 cut(s) 972
FaqI GGGAC 3 cut(s) 149, 990, 1551
FauI CCCGC 2 cut(s) 922, 1721
FauNDI CATATG 2 cut(s) 661, 1559
FbaI TGATCA 2 cut(s) 1321, 1465
FblI GTMKAC 1 cut(s) 1985
Fnu4HI GCNGC 5 cut(s) 927, 1165, 1604, 1880, 2047
FokI GGATG 8 cut(s) 66, 143, 165, 318, 564, 612, 1196, 1829
FriOI GRGCYC 1 cut(s) 1370
Fsp4HI GCNGC 5 cut(s) 927, 1165, 1604, 1880, 2047
FspBI CTAG 5 cut(s) 984, 1062, 1524, 1554, 2133
GlaI GCGC 1 cut(s) 773
GluI GCNGC 5 cut(s) 927, 1165, 1604, 1880, 2047
GsaI CCCAGC 1 cut(s) 276
GsuI CTGGAG 3 cut(s) 30, 1986, 2060
HaeIII GGCC 4 cut(s) 353, 424, 1315, 1971
HapII CCGG 2 cut(s) 764, 1152
HhaI GCGC 1 cut(s) 774
Hin6I GCGC 1 cut(s) 772
HinP1I GCGC 1 cut(s) 772
HincII GTYRAC 1 cut(s) 1120
HindII GTYRAC 1 cut(s) 1120
HindIII AAGCTT 2 cut(s) 1790, 1952
HpaII CCGG 2 cut(s) 764, 1152
HphI GGTGA 3 cut(s) 101, 1588, 1747
Hpy166II GTNNAC 3 cut(s) 204, 1120, 1986
Hpy188I TCNGA 8 cut(s) 1082, 1108, 1209, 1321, 1674, 1787, 2012, 2082
Hpy8I GTNNAC 3 cut(s) 204, 1120, 1986
HpyAV CCTTC 5 cut(s) 142, 299, 496, 1401, 1715
HpyCH4III ACNGT 6 cut(s) 376, 695, 1137, 1600, 1983, 2160
HpyCH4IV ACGT 1 cut(s) 642
HpyF10VI GCNNNNNNNGC 8 cut(s) 655, 1778, 1811, 1885, 2003, 2029, 2055, 2129
HpyF3I CTNAG 8 cut(s) 426, 681, 909, 1079, 1199, 1206, 1346, 1697
HpySE526I ACGT 1 cut(s) 642
HspAI GCGC 1 cut(s) 772
Kpn2I TCCGGA 1 cut(s) 1151
Ksp22I TGATCA 2 cut(s) 1321, 1465
LmnI GCTCC 1 cut(s) 517
Lsp1109I GCAGC 5 cut(s) 938, 1176, 1615, 1866, 2033
LweI GCATC 5 cut(s) 143, 165, 590, 1665, 1678
MaeI CTAG 5 cut(s) 984, 1062, 1524, 1554, 2133
MaeII ACGT 1 cut(s) 642
MaeIII GTNAC 4 cut(s) 844, 933, 1349, 1774
MfeI CAATTG 2 cut(s) 447, 1575
MflI RGATCY 7 cut(s) 5, 53, 129, 262, 413, 1669, 1861
MhlI GDGCHC 1 cut(s) 1370
MlsI TGGCCA 1 cut(s) 353
MluNI TGGCCA 1 cut(s) 353
MlyI GAGTC 7 cut(s) 636, 788, 1013, 1250, 1335, 1405, 2022
MmeI TCCRAC 1 cut(s) 1990
Mox20I TGGCCA 1 cut(s) 353
Mph1103I ATGCAT 3 cut(s) 158, 578, 1693
MroI TCCGGA 1 cut(s) 1151
MroXI GAANNNNTTC 2 cut(s) 862, 1592
MscI TGGCCA 1 cut(s) 353
MseI TTAA 8 cut(s) 198, 294, 654, 861, 893, 1374, 1764, 2141
MslI CAYNNNNRTG 2 cut(s) 462, 1926
Msp20I TGGCCA 1 cut(s) 353
MspA1I CMGCKG 3 cut(s) 60, 929, 2006
MspI CCGG 2 cut(s) 764, 1152
MspR9I CCNGG 6 cut(s) 421, 514, 765, 1404, 1421, 1835
MunI CAATTG 2 cut(s) 447, 1575
Mva1269I GAATGC 3 cut(s) 576, 1258, 1691
MvaI CCWGG 5 cut(s) 421, 514, 1404, 1421, 1835
MwoI GCNNNNNNNGC 8 cut(s) 655, 1778, 1811, 1885, 2003, 2029, 2055, 2129
NciI CCSGG 1 cut(s) 765
NdeI CATATG 2 cut(s) 661, 1559
NlaIV GGNNCC 4 cut(s) 109, 519, 762, 1617
NmeAIII GCCGAG 1 cut(s) 1001
NmuCI GTSAC 2 cut(s) 933, 1774
NsiI ATGCAT 3 cut(s) 158, 578, 1693
NspI RCATGY 3 cut(s) 834, 1642, 1925
NspV TTCGAA 1 cut(s) 869
PaeI GCATGC 1 cut(s) 1925
PagI TCATGA 3 cut(s) 532, 580, 1684
PaqCI CACCTGC 1 cut(s) 1720
PceI AGGCCT 1 cut(s) 424
PciI ACATGT 1 cut(s) 1638
PctI GAATGC 3 cut(s) 576, 1258, 1691
PdmI GAANNNNTTC 2 cut(s) 862, 1592
PfeI GAWTC 7 cut(s) 536, 729, 824, 919, 1040, 1148, 2077
PfoI TCCNGGA 1 cut(s) 1833
PkrI GCNGC 5 cut(s) 928, 1166, 1605, 1881, 2048
PleI GAGTC 7 cut(s) 635, 788, 1013, 1249, 1335, 1405, 2021
PpsI GAGTC 7 cut(s) 635, 788, 1013, 1249, 1335, 1405, 2021
PscI ACATGT 1 cut(s) 1638
PshBI ATTAAT 1 cut(s) 861
Psp6I CCWGG 5 cut(s) 419, 512, 1402, 1419, 1833
PspFI CCCAGC 1 cut(s) 272
PspGI CCWGG 5 cut(s) 419, 512, 1402, 1419, 1833
PspN4I GGNNCC 4 cut(s) 109, 519, 762, 1617
PspPI GGNCC 4 cut(s) 761, 1314, 1487, 1970
PstI CTGCAG 3 cut(s) 928, 1731, 2025
PsuI RGATCY 7 cut(s) 5, 53, 129, 262, 413, 1669, 1861
PvuII CAGCTG 1 cut(s) 60
RsaI GTAC 6 cut(s) 193, 441, 993, 1401, 1702, 1809
RsaNI GTAC 6 cut(s) 192, 440, 992, 1400, 1701, 1808
RseI CAYNNNNRTG 2 cut(s) 462, 1926
SaqAI TTAA 8 cut(s) 198, 294, 654, 861, 893, 1374, 1764, 2141
SatI GCNGC 5 cut(s) 927, 1165, 1604, 1880, 2047
Sau96I GGNCC 4 cut(s) 761, 1314, 1487, 1970
ScaI AGTACT 1 cut(s) 193
SchI GAGTC 7 cut(s) 636, 788, 1013, 1250, 1335, 1405, 2022
ScrFI CCNGG 6 cut(s) 421, 514, 765, 1404, 1421, 1835
SduI GDGCHC 1 cut(s) 1370
SfaNI GCATC 5 cut(s) 143, 165, 590, 1665, 1678
SfcI CTRYAG 6 cut(s) 321, 432, 924, 1218, 1727, 2021
SfuI TTCGAA 1 cut(s) 869
SinI GGWCC 2 cut(s) 761, 1487
SmiMI CAYNNNNRTG 2 cut(s) 462, 1926
SmlI CTYRAG 3 cut(s) 180, 338, 1307
SmoI CTYRAG 3 cut(s) 180, 338, 1307
SphI GCATGC 1 cut(s) 1925
SseBI AGGCCT 1 cut(s) 424
SsiI CCGC 5 cut(s) 929, 1714, 1805, 1814, 2006
SspMI CTAG 5 cut(s) 984, 1062, 1524, 1554, 2133
StuI AGGCCT 1 cut(s) 424
StyD4I CCNGG 6 cut(s) 419, 512, 763, 1402, 1419, 1833
StyI CCWWGG 1 cut(s) 972
TaaI ACNGT 6 cut(s) 376, 695, 1137, 1600, 1983, 2160
TaiI ACGT 1 cut(s) 645
TaqI TCGA 6 cut(s) 217, 613, 699, 869, 997, 1708
TaqII GACCGA 1 cut(s) 306
TatI WGTACW 1 cut(s) 191
TfiI GAWTC 7 cut(s) 536, 729, 824, 919, 1040, 1148, 2077
Tru1I TTAA 8 cut(s) 198, 294, 654, 861, 893, 1374, 1764, 2141
Tru9I TTAA 8 cut(s) 198, 294, 654, 861, 893, 1374, 1764, 2141
TscAI CASTG 4 cut(s) 1036, 1197, 1783, 1980
TseFI GTSAC 2 cut(s) 933, 1774
TseI GCWGC 5 cut(s) 926, 1164, 1603, 1879, 2046
Tsp45I GTSAC 2 cut(s) 933, 1774
TspDTI ATGAA 5 cut(s) 68, 318, 320, 569, 966
TspGWI ACGGA 3 cut(s) 128, 741, 1768
TspRI CASTG 4 cut(s) 1036, 1197, 1783, 1980
VpaK11BI GGWCC 2 cut(s) 761, 1487
VspI ATTAAT 1 cut(s) 861
XapI RAATTY 7 cut(s) 74, 296, 380, 616, 1286, 1623, 2085
XceI RCATGY 3 cut(s) 834, 1642, 1925
XmiI GTMKAC 1 cut(s) 1985
XmnI GAANNNNTTC 2 cut(s) 862, 1592
XspI CTAG 5 cut(s) 984, 1062, 1524, 1554, 2133
ZrmI AGTACT 1 cut(s) 193
Zsp2I ATGCAT 3 cut(s) 158, 578, 1693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.