MD05G1322800.v1.1

May participate in a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001097
Physical Location & Seq
Forward (+)
45004300 .. 45011941
7642 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1322800.v1.1.491

Sequence Viewer

Length: 2442 bp
ATGGCCAAGCGTGCTTACAAGCTGCAGGAATTTGTGGCGCATTCAGACAGTATTAATTGTCTAAGTATTGGGAAAAAGGCGTGTCGGCTTTTTGTTACTGGTGGTGATGATCACATAGTCAATCTCTGGGCAATTGGCAAACCCACTGCTTTGATGAGGTTTGCAGGTCATACAAGTCCAGTTGAATCCGTAGCTTTTAATTCGGGAGAAGTTTTGGTGCTTGCTGGAGCTTCGTCTGGTGTGACAAAAATATGGGATCTTGAGGAAACAAAGATGGTTCGCACACTAACTGGACACAGATCCAGCTGTACTGCTGTTGAATTTCATCCATTTGGTGAGTTCTTTGCTTCTGGTTCCATGGACACGAATCTAAAGATATGGGATATTAGAAAGAAGGGATGCATCCACACATACAAGGGTCATACTCAGGGCATTAGTACTATTAAATTCACTCCCGATGGGCGCTGGGTAGTTTCTGGTGGGTTTGACAATGCTGTAAAGATATGGGATCTAACTGCTGGGAAGCTCTTGCATGATTTTAAATTCCATGAAGGACATATTCGATCCTTAGATTTTCATCCCCTTGAGTTTCTTTTGGCCACAGGTTCAACAGACAGAACTGTAAAATTTTGGGATTTGGAAACTTTTGAATTGATTGGATCATCCAGACCTGAGCCTACAGGAGTACGTGCAATTGCTTTTCATCCTGATGGGAGGACCCTATTCTCAGGATTGGACGACAGTTTGAAGGTTTATTCATGGGAACCTGTAATATGTCATGATTCTGTTGATATGGGATGGAACAGACTTAGTGACTTATGCATTCATGATGGGAAACTGTTGGGCTGTTCATACTATCGAAATTCTGTTGGAGTTTGGGTTGCGGATATATCGCTTACTAAGCCATATGCAGCTGATTCAACGCCTGGGCATGATGACTGTGCAGATGAGAAACGTTCCGTTAAACTCCCGGAAATTGCAGGGAAGGTAGAGAGTGGTATAAGAAGTCCGGGCTTGCGGTGTATGTCGCCTGATTATGACACAAAGGAAATAAAAAACATATATGTGGATTGTAGTGGAGGAAAGCCAGTTGCCACACGGAGAATGGATTCTCCAAAGGTTGTACCCTCGTCGGATTCAAAGGCAAACAGTGCTGCAAAGCAGAATCCTTCGGTACGGTTGCCTCCAAAATCTAATGAACAAATAGTCAATAAATCACTGGCTGTGCCTAGTATTGTACCGCGGAGCAGTCCTGATGAAAAAGATACAGCTAACCCTGGAGGGGATGGTGTTGCACTTTCGAGGACCAAGCGTGGTATGTTGCTCAAACCTGCTCATGCAAGAAGGCCATCAAATAGCAAAATTGACTTCGAGAAGCTGCCGTTAGATAATGCAGTGGATCTTAACTCTCAAACCAAAGGTGTATCAGAAGATAGGGCTAGAGAGTCCAGTGAGGAAAAGCATTCTGAGATCAAGAGTGTTGAAGAGAAATTTGAAAAAGTATTTTCACCAAATAATCCCTCCAATCTTGAAGCCAGAAACGGATCCCTCAATTGCAGCAAGGACACGAACTCTGTTAAATTTGTAAATGGAGTTGCCGTTGTTTCAGGAAGGACTCGCTCCCTGGTTGAGCGGTTTGAAAGGAGAGAGAGATTTGATGGTAATGAAGATCAAGCAACTAGCATATTGAGTCCTGTGATACCTGAAGATCAAACAACTTGCATATCTAGTCCTGTGATACCTAAAGATCAAGCAACTAGCGTAGCTAGTCCTGTGATACCCGAAGTTGAAGCAAGCATCTGTAGTCCTGTGATACCTGAAGTTGAAGCTAACATCTCTAGACCTGTGATACCTGAAAGGGACAGAGCATCCACCATGCTGAAGGAAGGACCTCAAATTTCTGGGAGGCATATGATCTGTACGAACAATGAGGGTCCTCTTGAAGATTTGATGCAAACTCACGACGTATTTCTCAGTACCCTTCGTTCCCGCTTGACAAAACTGCAGGTGGTGCGGCATTTTTGGGAACAGAATGATATTAAAGGTGCTATCGGTGCTATCAGGAAGCTGCCAGATCATTCTGTACAAGCGGATGTTGTTACTGTTCTTCTGGAGAAAATGGATTGTCTCACATTAGATCTGTTTTCTTGTTTGCTTCCTGTGCTTCTGGGCTTACTCGATAGCAAGACAGAAAGGCATGCAAATATTTCGTTGGAGATGCTATTGAAGCTAGTGGCAGTCTTTGGCCCAGTCATACACTCAACTGTTTCAGGACCTCGACCTGTCGGTGTCAACCTTCAGGCAGAGCAAAGGCTGGAATGCTGCAAGCAATGCTCCATCCATCTTCAAAAGATCCAGAAAATTCTTCCGGTCTTTGTCAGGAGGGGTGGTGTGCTGGCAAGATGTGCTCAGGAATTGAATCTAATTATTCAACAGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Katanin_con80 WD40

Protein Analysis

814

Amino Acids

89.45

Weight (kDa)

7.55

Isoelectric Point (pI)

47.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_MABP1-WDR62_2nd PF24782 6 - 171 2.6e-10 MABP1/WDR62 second WD40 domain
Beta-prop_THOC3 PF25174 7 - 75 3.3e-07 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 8 - 137 3.6e-25 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 9 - 129 7.7e-21 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 10 - 133 2.4e-14 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 12 - 132 7.7e-11 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 15 - 127 2.3e-07 WDR36/Utp21 first beta-propeller
WD40_Prp19 PF24814 15 - 254 3.3e-46 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 23 - 140 7.5e-16 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 29 - 178 3.2e-18 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 31 - 132 4.3e-07 WDR90/POC16, second beta-propeller
Beta-prop_WDR3_2nd PF25172 52 - 173 8.6e-18 WDR3 second beta-propeller domain
EIF3I PF24805 54 - 138 1.6e-06 EIF3I
Beta-prop_SPT8 PF23798 55 - 152 3.7e-06 SPT8 beta propeller
WD40_CDC20-Fz PF24807 60 - 245 9.2e-31 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 66 - 200 5.1e-30 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 84 - 253 2.9e-37 WDR3 first beta-propeller domain
WDR55 PF24796 89 - 255 5.1e-15 WDR55
WD40 PF00400 91 - 128 7.7e-10 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 92 - 218 1.6e-10 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 95 - 256 4.2e-36 WDR5 beta-propeller domain
Beta-prop_DCAF4 PF23761 99 - 178 6.6e-06 DDB1- and CUL4-associated factor 4 beta-propeller domain
Beta-prop_Aladin PF25460 100 - 199 1.1e-07 Aladin seven-bladed propeller
Beta-prop_SCAP PF24017 117 - 202 1.4e-06 SCAP Beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 118 - 252 8.5e-12 WDR36/Utp21 first beta-propeller
WD40_Gbeta PF25391 119 - 256 6.2e-17 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 128 - 201 2.1e-11 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 134 - 170 1e-11 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 136 - 253 9e-06 TEP-1 second beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 136 - 219 6.6e-11 CAF1B/HIR1 beta-propeller domain
Beta-prop_EML PF23409 138 - 240 1e-06 Echinoderm microtubule-associated protein first beta-propeller
EIF3I PF24805 139 - 249 1.1e-12 EIF3I
WD40_WDHD1_1st PF24817 142 - 253 1.8e-10 WDHD1 first WD40 domain
WD40_MABP1-WDR62_2nd PF24782 143 - 214 4.3e-06 MABP1/WDR62 second WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 163 - 245 8.6e-10 WDR90/POC16, second beta-propeller
Beta-prop_WDR3_2nd PF25172 166 - 217 8.6e-06 WDR3 second beta-propeller domain
WD40 PF00400 174 - 212 8.6e-08 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 175 - 248 3.5e-10 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 189 - 247 6.5e-07 Echinoderm microtubule-associated protein second beta-propeller
Katanin_con80 PF13925 652 - 809 4.7e-52 con80 domain of Katanin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1996
Acc36I ACCTGC 3 cut(s) 155, 1339, 1996
AccBSI CCGCTC 1 cut(s) 1633
AccII CGCG 1 cut(s) 1243
AciI CCGC 8 cut(s) 884, 1018, 1241, 1243, 1633, 1990, 2014, 2090
AclI AACGTT 1 cut(s) 955
AclWI GGATC 9 cut(s) 264, 294, 516, 558, 667, 1407, 1539, 1552, 2347
AcoI YGGCCR 2 cut(s) 3, 597
AcuI CTGAAG 4 cut(s) 1725, 1839, 1901, 2282
AfaI GTAC 9 cut(s) 310, 439, 687, 1125, 1176, 1239, 1921, 1978, 2085
AfiI CCNNNNNNNGG 1 cut(s) 1282
AjnI CCWGG 3 cut(s) 925, 1276, 1623
Alw21I GWGCWC 1 cut(s) 2410
Alw26I GTCTC 1 cut(s) 2132
AlwI GGATC 9 cut(s) 264, 294, 516, 558, 667, 1407, 1539, 1552, 2347
AoxI GGCC 4 cut(s) 3, 597, 1346, 2245
ApeKI GCWGC 7 cut(s) 22, 911, 1154, 1378, 1557, 2068, 2322
AseI ATTAAT 1 cut(s) 54
Asp700I GAANNNNTTC 1 cut(s) 1108
AspLEI GCGC 2 cut(s) 40, 465
AspS9I GGNCC 6 cut(s) 717, 1305, 1889, 1934, 2246, 2273
AsuC2I CCSGG 2 cut(s) 971, 1011
AsuHPI GGTGA 3 cut(s) 116, 347, 1500
AvaII GGWCC 5 cut(s) 717, 1305, 1889, 1934, 2273
BalI TGGCCA 2 cut(s) 5, 599
BamHI GGATCC 1 cut(s) 1544
Bbv12I GWGCWC 1 cut(s) 2410
BbvI GCAGC 7 cut(s) 9, 923, 1141, 1365, 1569, 2055, 2309
BceAI ACGGC 2 cut(s) 1366, 1583
BcgI CGANNNNNNTGC 2 cut(s) 2190, 2224
BciT130I CCWGG 3 cut(s) 927, 1278, 1625
BclI TGATCA 1 cut(s) 109
BcnI CCSGG 2 cut(s) 971, 1011
BcoDI GTCTC 1 cut(s) 2132
BfaI CTAG 8 cut(s) 1230, 1440, 1680, 1728, 1758, 1767, 1839, 2231
BfmI CTRYAG 4 cut(s) 23, 678, 1801, 2003
BfoI RGCGCY 1 cut(s) 466
BfuAI ACCTGC 3 cut(s) 155, 1339, 1996
BglII AGATCT 1 cut(s) 2137
BisI GCNGC 8 cut(s) 23, 912, 1155, 1379, 1558, 2015, 2069, 2323
BlsI GCNGC 8 cut(s) 24, 913, 1156, 1380, 1559, 2016, 2070, 2324
BmcAI AGTACT 1 cut(s) 439
Bme1390I CCNGG 5 cut(s) 927, 971, 1011, 1278, 1625
Bme18I GGWCC 5 cut(s) 717, 1305, 1889, 1934, 2273
BmgT120I GGNCC 6 cut(s) 717, 1305, 1889, 1934, 2246, 2273
BmiI GGNNCC 5 cut(s) 355, 719, 765, 1546, 1935
BmrFI CCNGG 5 cut(s) 927, 971, 1011, 1278, 1625
BmrI ACTGGG 1 cut(s) 2243
BmsI GCATC 6 cut(s) 389, 411, 1806, 1877, 1941, 2208
BmuI ACTGGG 1 cut(s) 2243
BpmI CTGGAG 3 cut(s) 246, 1299, 2132
Bpu10I CCTNAGC 2 cut(s) 672, 2409
BpuEI CTTGAG 2 cut(s) 281, 605
BpuMI CCSGG 2 cut(s) 971, 1011
BsaAI YACGTR 1 cut(s) 689
BsaBI GATNNNNATC 1 cut(s) 576
BsaJI CCNNGG 5 cut(s) 357, 926, 1241, 1276, 1623
BsaWI WCCGGW 1 cut(s) 2368
BsaXI ACNNNNNCTCC 6 cut(s) 864, 894, 1584, 1614, 2374, 2404
Bsc4I CCNNNNNNNGG 1 cut(s) 1282
Bse1I ACTGG 7 cut(s) 103, 179, 295, 1088, 1224, 1449, 2249
Bse3DI GCAATG 1 cut(s) 2336
Bse8I GATNNNNATC 1 cut(s) 576
BseBI CCWGG 3 cut(s) 927, 1278, 1625
BseDI CCNNGG 5 cut(s) 357, 926, 1241, 1276, 1623
BseJI GATNNNNATC 1 cut(s) 576
BseLI CCNNNNNNNGG 1 cut(s) 1282
BseMI GCAATG 1 cut(s) 2336
BseMII CTCAG 6 cut(s) 440, 663, 741, 1458, 1987, 2423
BseNI ACTGG 7 cut(s) 103, 179, 295, 1088, 1224, 1449, 2249
BseXI GCAGC 7 cut(s) 9, 923, 1141, 1365, 1569, 2055, 2309
BseYI CCCAGC 2 cut(s) 465, 518
BsgI GTGCAG 1 cut(s) 963
Bsh1236I CGCG 1 cut(s) 1243
BshFI GGCC 4 cut(s) 5, 599, 1348, 2247
BsiHKAI GWGCWC 1 cut(s) 2410
BsiSI CCGG 3 cut(s) 971, 1010, 2369
BslFI GGGAC 1 cut(s) 1874
BslI CCNNNNNNNGG 1 cut(s) 1282
BsmAI GTCTC 1 cut(s) 2132
BsmFI GGGAC 1 cut(s) 1874
BsmI GAATGC 4 cut(s) 40, 822, 1462, 2324
BsnI GGCC 4 cut(s) 5, 599, 1348, 2247
Bsp1286I GDGCHC 1 cut(s) 2410
Bsp1407I TGTACA 1 cut(s) 2083
Bsp19I CCATGG 1 cut(s) 357
BspACI CCGC 8 cut(s) 884, 1018, 1241, 1243, 1633, 1990, 2014, 2090
BspANI GGCC 4 cut(s) 5, 599, 1348, 2247
BspCNI CTCAG 6 cut(s) 439, 664, 740, 1459, 1986, 2422
BspFNI CGCG 1 cut(s) 1243
BspHI TCATGA 2 cut(s) 778, 826
BspLI GGNNCC 5 cut(s) 355, 719, 765, 1546, 1935
BspMAI CTGCAG 2 cut(s) 27, 2007
BspMI ACCTGC 3 cut(s) 155, 1339, 1996
BspPI GGATC 9 cut(s) 264, 294, 516, 558, 667, 1407, 1539, 1552, 2347
BsrBI CCGCTC 1 cut(s) 1633
BsrDI GCAATG 1 cut(s) 2336
BsrGI TGTACA 1 cut(s) 2083
BsrI ACTGG 7 cut(s) 103, 179, 295, 1088, 1224, 1449, 2249
BssECI CCNNGG 5 cut(s) 357, 926, 1241, 1276, 1623
BssT1I CCWWGG 1 cut(s) 357
Bst2UI CCWGG 3 cut(s) 927, 1278, 1625
Bst6I CTCTTC 1 cut(s) 1479
BstAPI GCANNNNNTGC 4 cut(s) 1151, 2011, 2331, 2405
BstAUI TGTACA 1 cut(s) 2083
BstBAI YACGTR 1 cut(s) 689
BstC8I GCNNGC 7 cut(s) 12, 222, 1016, 1795, 2199, 2327, 2397
BstDSI CCRYGG 2 cut(s) 357, 1241
BstFNI CGCG 1 cut(s) 1243
BstH2I RGCGCY 1 cut(s) 466
BstHHI GCGC 2 cut(s) 40, 465
BstMAI GTCTC 1 cut(s) 2132
BstMWI GCNNNNNNNGC 9 cut(s) 11, 901, 1151, 2011, 2054, 2161, 2227, 2331, 2405
BstNI CCWGG 3 cut(s) 927, 1278, 1625
BstNSI RCATGY 1 cut(s) 2201
BstSCI CCNGG 5 cut(s) 925, 969, 1009, 1276, 1623
BstSFI CTRYAG 4 cut(s) 23, 678, 1801, 2003
BstUI CGCG 1 cut(s) 1243
BstV1I GCAGC 7 cut(s) 9, 923, 1141, 1365, 1569, 2055, 2309
BstX2I RGATCY 7 cut(s) 256, 299, 508, 1399, 1544, 2137, 2352
BstYI RGATCY 7 cut(s) 256, 299, 508, 1399, 1544, 2137, 2352
BsuRI GGCC 4 cut(s) 5, 599, 1348, 2247
BtgI CCRYGG 2 cut(s) 357, 1241
BtsI GCAGTG 2 cut(s) 144, 1401
BtsIMutI CAGTG 5 cut(s) 144, 1156, 1217, 1401, 1456
BveI ACCTGC 3 cut(s) 155, 1339, 1996
Cac8I GCNNGC 7 cut(s) 12, 222, 1016, 1795, 2199, 2327, 2397
CciI TCATGA 2 cut(s) 778, 826
CfoI GCGC 2 cut(s) 40, 465
Cfr13I GGNCC 6 cut(s) 717, 1305, 1889, 1934, 2246, 2273
Cfr42I CCGCGG 1 cut(s) 1244
Csp6I GTAC 9 cut(s) 309, 438, 686, 1124, 1175, 1238, 1920, 1977, 2084
CviQI GTAC 9 cut(s) 309, 438, 686, 1124, 1175, 1238, 1920, 1977, 2084
DraI TTTAAA 1 cut(s) 541
EaeI YGGCCR 2 cut(s) 3, 597
Eam1104I CTCTTC 1 cut(s) 1479
EarI CTCTTC 1 cut(s) 1479
Eco130I CCWWGG 1 cut(s) 357
Eco47I GGWCC 5 cut(s) 717, 1305, 1889, 1934, 2273
Eco57I CTGAAG 4 cut(s) 1725, 1839, 1901, 2282
EcoO109I RGGNCCY 4 cut(s) 717, 1889, 1934, 2273
EcoRII CCWGG 3 cut(s) 925, 1276, 1623
EcoT14I CCWWGG 1 cut(s) 357
EcoT22I ATGCAT 2 cut(s) 404, 824
ErhI CCWWGG 1 cut(s) 357
FaqI GGGAC 1 cut(s) 1874
FauI CCCGC 1 cut(s) 1997
FauNDI CATATG 2 cut(s) 907, 1911
FbaI TGATCA 1 cut(s) 109
Fnu4HI GCNGC 8 cut(s) 23, 912, 1155, 1379, 1558, 2015, 2069, 2323
Fsp4HI GCNGC 8 cut(s) 23, 912, 1155, 1379, 1558, 2015, 2069, 2323
FspBI CTAG 8 cut(s) 1230, 1440, 1680, 1728, 1758, 1767, 1839, 2231
GlaI GCGC 2 cut(s) 39, 464
GluI GCNGC 8 cut(s) 23, 912, 1155, 1379, 1558, 2015, 2069, 2323
GsaI CCCAGC 2 cut(s) 469, 522
GsuI CTGGAG 3 cut(s) 246, 1299, 2132
HaeII RGCGCY 1 cut(s) 466
HaeIII GGCC 4 cut(s) 5, 599, 1348, 2247
HapII CCGG 3 cut(s) 971, 1010, 2369
HhaI GCGC 2 cut(s) 40, 465
Hin6I GCGC 2 cut(s) 38, 463
HinP1I GCGC 2 cut(s) 38, 463
HincII GTYRAC 1 cut(s) 2293
HindII GTYRAC 1 cut(s) 2293
HpaII CCGG 3 cut(s) 971, 1010, 2369
HphI GGTGA 3 cut(s) 116, 347, 1500
Hpy166II GTNNAC 1 cut(s) 2293
Hpy188I TCNGA 4 cut(s) 46, 1135, 1429, 1468
Hpy8I GTNNAC 1 cut(s) 2293
Hpy99I CGWCG 2 cut(s) 1135, 1967
HpyCH4IV ACGT 3 cut(s) 688, 955, 1965
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 901, 1151, 2011, 2054, 2161, 2227, 2331, 2405
HpySE526I ACGT 3 cut(s) 688, 955, 1965
HspAI GCGC 2 cut(s) 38, 463
Ksp22I TGATCA 1 cut(s) 109
KspI CCGCGG 1 cut(s) 1244
LmnI GCTCC 4 cut(s) 227, 1245, 1625, 2339
Lsp1109I GCAGC 7 cut(s) 9, 923, 1141, 1365, 1569, 2055, 2309
LweI GCATC 6 cut(s) 389, 411, 1806, 1877, 1941, 2208
MaeI CTAG 8 cut(s) 1230, 1440, 1680, 1728, 1758, 1767, 1839, 2231
MaeII ACGT 3 cut(s) 688, 955, 1965
MaeIII GTNAC 4 cut(s) 94, 241, 812, 2098
MbiI CCGCTC 1 cut(s) 1633
MboII GAAGA 8 cut(s) 1442, 1496, 1679, 1718, 1955, 2099, 2336, 2357
MfeI CAATTG 3 cut(s) 132, 693, 1552
MflI RGATCY 7 cut(s) 256, 299, 508, 1399, 1544, 2137, 2352
MhlI GDGCHC 1 cut(s) 2410
MlsI TGGCCA 2 cut(s) 5, 599
MluNI TGGCCA 2 cut(s) 5, 599
MlyI GAGTC 3 cut(s) 1454, 1609, 1699
MmeI TCCRAC 3 cut(s) 850, 1113, 2193
Mox20I TGGCCA 2 cut(s) 5, 599
Mph1103I ATGCAT 2 cut(s) 404, 824
MroXI GAANNNNTTC 1 cut(s) 1108
MscI TGGCCA 2 cut(s) 5, 599
MseI TTAA 8 cut(s) 54, 198, 444, 540, 963, 1404, 1578, 2040
MslI CAYNNNNRTG 2 cut(s) 708, 1064
Msp20I TGGCCA 2 cut(s) 5, 599
MspA1I CMGCKG 3 cut(s) 306, 914, 1243
MspI CCGG 3 cut(s) 971, 1010, 2369
MspR9I CCNGG 5 cut(s) 927, 971, 1011, 1278, 1625
MunI CAATTG 3 cut(s) 132, 693, 1552
Mva1269I GAATGC 4 cut(s) 40, 822, 1462, 2324
MvaI CCWGG 3 cut(s) 927, 1278, 1625
MvnI CGCG 1 cut(s) 1243
MwoI GCNNNNNNNGC 9 cut(s) 11, 901, 1151, 2011, 2054, 2161, 2227, 2331, 2405
NciI CCSGG 2 cut(s) 971, 1011
NcoI CCATGG 1 cut(s) 357
NdeI CATATG 2 cut(s) 907, 1911
NlaIV GGNNCC 5 cut(s) 355, 719, 765, 1546, 1935
NmuCI GTSAC 2 cut(s) 241, 812
NsiI ATGCAT 2 cut(s) 404, 824
NspI RCATGY 1 cut(s) 2201
PaeI GCATGC 1 cut(s) 2201
PagI TCATGA 2 cut(s) 778, 826
PaqCI CACCTGC 1 cut(s) 1996
PctI GAATGC 4 cut(s) 40, 822, 1462, 2324
PdmI GAANNNNTTC 1 cut(s) 1108
PfeI GAWTC 8 cut(s) 185, 367, 782, 917, 1109, 1136, 1165, 2419
PfoI TCCNGGA 1 cut(s) 969
PkrI GCNGC 8 cut(s) 24, 913, 1156, 1380, 1559, 2016, 2070, 2324
PleI GAGTC 3 cut(s) 1453, 1609, 1698
PpsI GAGTC 3 cut(s) 1453, 1609, 1698
Ppu21I YACGTR 1 cut(s) 689
PpuMI RGGWCCY 4 cut(s) 717, 1889, 1934, 2273
PshBI ATTAAT 1 cut(s) 54
Psp1406I AACGTT 1 cut(s) 955
Psp5II RGGWCCY 4 cut(s) 717, 1889, 1934, 2273
Psp6I CCWGG 3 cut(s) 925, 1276, 1623
PspFI CCCAGC 2 cut(s) 465, 518
PspGI CCWGG 3 cut(s) 925, 1276, 1623
PspN4I GGNNCC 5 cut(s) 355, 719, 765, 1546, 1935
PspPI GGNCC 6 cut(s) 717, 1305, 1889, 1934, 2246, 2273
PspPPI RGGWCCY 4 cut(s) 717, 1889, 1934, 2273
PsrI GAACNNNNNNTAC 2 cut(s) 1969, 2001
PstI CTGCAG 2 cut(s) 27, 2007
PsuI RGATCY 7 cut(s) 256, 299, 508, 1399, 1544, 2137, 2352
PvuII CAGCTG 2 cut(s) 306, 914
RsaI GTAC 9 cut(s) 310, 439, 687, 1125, 1176, 1239, 1921, 1978, 2085
RsaNI GTAC 9 cut(s) 309, 438, 686, 1124, 1175, 1238, 1920, 1977, 2084
RseI CAYNNNNRTG 2 cut(s) 708, 1064
SacII CCGCGG 1 cut(s) 1244
SaqAI TTAA 8 cut(s) 54, 198, 444, 540, 963, 1404, 1578, 2040
SatI GCNGC 8 cut(s) 23, 912, 1155, 1379, 1558, 2015, 2069, 2323
Sau96I GGNCC 6 cut(s) 717, 1305, 1889, 1934, 2246, 2273
ScaI AGTACT 1 cut(s) 439
SchI GAGTC 3 cut(s) 1454, 1609, 1699
ScrFI CCNGG 5 cut(s) 927, 971, 1011, 1278, 1625
SduI GDGCHC 1 cut(s) 2410
SfaNI GCATC 6 cut(s) 389, 411, 1806, 1877, 1941, 2208
SfcI CTRYAG 4 cut(s) 23, 678, 1801, 2003
Sfr303I CCGCGG 1 cut(s) 1244
SgrBI CCGCGG 1 cut(s) 1244
SinI GGWCC 5 cut(s) 717, 1305, 1889, 1934, 2273
SmiMI CAYNNNNRTG 2 cut(s) 708, 1064
SmlI CTYRAG 2 cut(s) 260, 584
SmoI CTYRAG 2 cut(s) 260, 584
SphI GCATGC 1 cut(s) 2201
SsiI CCGC 8 cut(s) 884, 1018, 1241, 1243, 1633, 1990, 2014, 2090
SspI AATATT 1 cut(s) 2206
SspMI CTAG 8 cut(s) 1230, 1440, 1680, 1728, 1758, 1767, 1839, 2231
StyD4I CCNGG 5 cut(s) 925, 969, 1009, 1276, 1623
StyI CCWWGG 1 cut(s) 357
TaiI ACGT 3 cut(s) 691, 958, 1968
TaqI TCGA 6 cut(s) 562, 859, 1301, 1371, 2178, 2278
TatI WGTACW 3 cut(s) 308, 437, 2083
TauI GCSGC 1 cut(s) 2017
TfiI GAWTC 8 cut(s) 185, 367, 782, 917, 1109, 1136, 1165, 2419
Tru1I TTAA 8 cut(s) 54, 198, 444, 540, 963, 1404, 1578, 2040
Tru9I TTAA 8 cut(s) 54, 198, 444, 540, 963, 1404, 1578, 2040
TscAI CASTG 5 cut(s) 151, 1156, 1224, 1401, 1456
TseFI GTSAC 2 cut(s) 241, 812
TseI GCWGC 7 cut(s) 22, 911, 1154, 1378, 1557, 2068, 2322
Tsp45I GTSAC 2 cut(s) 241, 812
TspGWI ACGGA 4 cut(s) 178, 949, 1114, 1557
TspRI CASTG 5 cut(s) 151, 1156, 1224, 1401, 1456
VpaK11BI GGWCC 5 cut(s) 717, 1305, 1889, 1934, 2273
VspI ATTAAT 1 cut(s) 54
XbaI TCTAGA 1 cut(s) 1838
XceI RCATGY 1 cut(s) 2201
XcmI CCANNNNNNNNNTGG 1 cut(s) 1102
XmnI GAANNNNTTC 1 cut(s) 1108
XspI CTAG 8 cut(s) 1230, 1440, 1680, 1728, 1758, 1767, 1839, 2231
ZrmI AGTACT 1 cut(s) 439
Zsp2I ATGCAT 2 cut(s) 404, 824
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.