RchiOBHm_Chr5g0007421

May participate in a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001097
Physical Location & Seq
Reverse (-)
4663755 .. 4672320
8566 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28847

Sequence Viewer

Length: 2448 bp
ATGGCGAAGCGTGCATACAAGCTGCAGGAGTTTGTGGCGCATTCTGACAATATTAACTGTCTAAATATTGGGAAAAAGGCGTGCCGACTTTATGTCACTGGCGGTGACGATCACAAAGTCAATCTATGGACAATTGGCAAACCTACTGCGTTAATGAGTCTCACTGGTCATACAAGTCCAGTTGAATCTGTAGCTTTTAATTCGGCAGAAGTTTTGGTTCTTGCTGGAGCTTCTACTGGTGCAACAAAAATATGGGATCTGGAGGAAACAAAGATGGTTCGCACACTTACTGGACACAGATCCAGCTGTTCAGCTGTTGAATTTCATCCATTTGGTGAGTTCTTTGCTTCTGGTTCCATGGACACTAATCTGAAGATCTGGGACATTAGAAAGAAGGGATGCATCCACACATACAAAGGTCATACCCAAGGCATTAGTAATATTAAGTTCACTCCCGATGGTCGATGGGTAGTTTCTGGTGGGTTTGACAATGTTGTAAAGGTGTGGGATCTAACTGCTGGGAAGCTCTTGCATGATTTTAAATTCCATGAAGGACATATTCGGTCTATAGATTTTCATCCCCTTGAGTTTCTCTTGGCGACAGGTTCAGCAGACAGAACTGTGAAATTCTGGGATCTGGAAACTTTTGAACTGATTGGATCTACCAGGCCTGAGCCTACAGGAGTACGCGCAATTACTTTCCACCCTGATGGGAGGACAATATTTTCTGGATTGGATGACAGTTTGAAGGTTTATTCATGGGAGCCTGTAATATCCCATGATTCTGTTGATATGGGATGGTCAAGACTTGCTGACTTATGCATTCATGACGGGAAGCTGTTGGGATGCTCTTACTATCGAAATTCTATTGGAGTCTGGGTTGCGGATGTGTCGCTTATTAAGCCATATGGAGCTGGTTCAACACCTGAGCAAAATGACTGTTTAGATGAGAAATCTCATGTAAAGTTGCAAGGAATCATGGAGAAGGTAGAGACTGGTATAAGGGGTCCGGGTTTGCGCTGTACATCTCCTGATTATGACACAAAGGAAATAAAGAACATATATGTTGATTCTGCAGGTGGAAAGCCAGTTACCTCACAGAGAATTAATTCTTCAAAAGTTTTACCCCCATTGGATTTAAAGGAAACCAGTGCTAAGAAGCAGATTCCTGCAGCAGGGTTGCTCCCAAAGTCTAATGAAAAGATGGTCAATAAATCCCTGGTTTCGCCTAGCATCGTACCTCGAAGCAGTCCCAATGCAAAAGATTCAGCTAACTCTGGAAGAGAATCTGTTGCATTTCCGAAAAGGCGTGCTATGTTGCTCAGACCAGCTCATGTGCGAAGCCCATCAAATAGCAAAGTTGACATGGAGAAGCTACCGTTGGCCGTTGAATCTGAAACTTTCAGCAGTATGACGAGAAAATTGGATGGTGCAGTGGGTCTCAGTTCTCAAATCAAAACTGTATCAGAAGATAGTGATGGAGAGGCCTGTAAGGAAAAACATTCTGCAATCAAGAGTGTAGAAGAGAAATTTGAAGCAGTATTATCACTGAAGAGGCCCTCTGATCAGGAAACCAGAAATAGATCCCTCAATAACAGCAAGGAAATGAGCCCTGTTAAAGTTGTCAATGGAGTTGCCGTTGTACCAGGAAGGACTCGCACCCTGGTTGAGAGATTTGAAAGGAGAGAGAGATTTAGTAGCAATGATGATCAAGCAACGGGCGTATTTGGTCCCGTGATACCTGAATATCAAGCAACCAGCATATCTTGTACTGTGATACCTGAAGATCAAGCAAGTAGCATATCTAGTCCTGTGATGCCTAAAGATCAAGCAACTAGCATATGTAGTCCCGTGATACATGAAATGGATAAAGCATTCACCATGCAGCGAGAAGAAGAACCTCAAATTTCTGGGAGGCACATGTCATCTGCAAACAATGATCCTGCTGAAGATCTGATGCAAACTCATGATGCATTCCTAAGTACCCTTCGATCCCGCCTGACAAAACTGCAGGTGGTGAGGCATTTTTGGGAACGAAATGATATTAAAGGTGCTATCAGTGCTATCAGAAAGCTGCCAGATCATTCGGTACAAGCAGATGTGATTAGTGTTCTCCTGGAGAAAATGGATATTCTGACATTAGATCTCTTTTCGTGTTTGCTGCCTGTGCTTCTGGGCTTACTTGATAGCAAAATTGAGAGGCATGCAAATGTGTCATTGGAGATGCTGTTGAAGCTTGTAGCAGTCTTTGGCCCAGTGATACGGTCTACCATTTCAGCACCTCCAGCGGTCGGAGTCAATCTTCAGGCAGAGCAAAGGCTGGAATGCTGCAACCAATGCTCTGTACACCTGCAAAAGATCCAGAAAATTCTCCCAGTCTTTGTAAGGAAGGGGGGCGTGCTGGCAAGATGTGCTCTAGAATTGAATCTAATTCTTCAAGAGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Katanin_con80 WD40

Protein Analysis

815

Amino Acids

89.8

Weight (kDa)

8.2

Isoelectric Point (pI)

47.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 7 - 69 8.4e-06 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 8 - 129 1.4e-09 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 8 - 137 1.8e-25 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 9 - 129 3.8e-21 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 10 - 133 4e-14 G protein beta WD-40 repeat protein
WD40_MABP1-WDR62_2nd PF24782 10 - 171 1.1e-09 MABP1/WDR62 second WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 12 - 127 1.1e-07 WDR36/Utp21 first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 12 - 135 1.7e-11 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 15 - 254 1e-45 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 22 - 140 5e-17 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 29 - 177 4.6e-18 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 32 - 132 8.8e-06 WDR90/POC16, second beta-propeller
Beta-prop_WDR3_2nd PF25172 51 - 173 1.6e-18 WDR3 second beta-propeller domain
EIF3I PF24805 52 - 138 4.9e-07 EIF3I
Beta-prop_CAF1B_HIR1 PF24105 52 - 137 2.1e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_EML PF23409 55 - 175 2.3e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_SPT8 PF23798 57 - 152 3.9e-06 SPT8 beta propeller
WD40_CDC20-Fz PF24807 60 - 245 2.4e-31 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 63 - 200 4e-30 THOC3 beta-propeller domain
WDR55 PF24796 66 - 255 1.2e-15 WDR55
WD40_MABP1-WDR62_1st PF24780 84 - 216 1.9e-09 MABP1/WDR62 first WD40 domain
WD40 PF00400 91 - 128 1.9e-09 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 91 - 218 2.6e-10 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 95 - 251 1.4e-36 WDR5 beta-propeller domain
Beta-prop_Aladin PF25460 101 - 173 1.2e-07 Aladin seven-bladed propeller
Beta-prop_WDR3_1st PF25173 106 - 253 8.5e-30 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 118 - 252 5e-11 WDR36/Utp21 first beta-propeller
WD40_Gbeta PF25391 120 - 255 1.6e-15 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 128 - 201 1.2e-11 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 134 - 170 1.8e-11 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 136 - 254 8.2e-06 TEP-1 second beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 136 - 219 5.7e-11 CAF1B/HIR1 beta-propeller domain
Beta-prop_EML PF23409 138 - 241 9.3e-07 Echinoderm microtubule-associated protein first beta-propeller
EIF3I PF24805 139 - 250 1.7e-14 EIF3I
WD40_WDHD1_1st PF24817 143 - 218 3.8e-10 WDHD1 first WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 163 - 245 1.9e-09 WDR90/POC16, second beta-propeller
Beta-prop_THOC3 PF25174 164 - 248 2e-13 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 167 - 217 3.3e-06 WDR3 second beta-propeller domain
WD40 PF00400 174 - 212 5.5e-08 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 189 - 247 2.2e-06 Echinoderm microtubule-associated protein second beta-propeller
Katanin_con80 PF13925 654 - 811 3.7e-53 con80 domain of Katanin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 1067, 2002, 2358
Acc36I ACCTGC 3 cut(s) 1067, 2002, 2358
AccI GTMKAC 1 cut(s) 2267
AccII CGCG 1 cut(s) 690
AciI CCGC 4 cut(s) 102, 884, 1996, 2288
AclWI GGATC 9 cut(s) 264, 294, 516, 642, 667, 1578, 1934, 1986, 2353
AcoI YGGCCR 1 cut(s) 1383
AcsI RAATTY 7 cut(s) 320, 542, 626, 862, 1529, 1905, 2367
AcuI CTGAAG 5 cut(s) 392, 1571, 1803, 1968, 2288
AfaI GTAC 8 cut(s) 687, 1024, 1239, 1644, 1771, 1984, 2091, 2346
AfiI CCNNNNNNNGG 2 cut(s) 1175, 2291
AflIII ACRYGT 1 cut(s) 1920
AhdI GACNNNNNGTC 1 cut(s) 92
AjnI CCWGG 5 cut(s) 665, 1218, 1645, 1662, 2115
AjuI GAANNNNNNNTTGG 2 cut(s) 1364, 1396
Alw21I GWGCWC 1 cut(s) 2416
Alw26I GTCTC 3 cut(s) 164, 986, 1445
AlwI GGATC 9 cut(s) 264, 294, 516, 642, 667, 1578, 1934, 1986, 2353
AoxI GGCC 5 cut(s) 668, 1383, 1485, 1556, 2251
ApeKI GCWGC 6 cut(s) 22, 1172, 1885, 2074, 2161, 2328
ApoI RAATTY 7 cut(s) 320, 542, 626, 862, 1529, 1905, 2367
AseI ATTAAT 1 cut(s) 1107
Asp700I GAANNNNTTC 1 cut(s) 1108
AspLEI GCGC 3 cut(s) 40, 692, 1020
AspS9I GGNCC 4 cut(s) 1007, 1557, 1730, 2252
AsuC2I CCSGG 1 cut(s) 1011
AsuHPI GGTGA 4 cut(s) 116, 347, 1870, 2029
AvaII GGWCC 2 cut(s) 1007, 1730
BanII GRGCYC 1 cut(s) 1613
BarI GAAGNNNNNNTAC 1 cut(s) 31
Bbv12I GWGCWC 1 cut(s) 2416
BbvI GCAGC 6 cut(s) 9, 1184, 1897, 2061, 2148, 2315
BccI CCATC 9 cut(s) 268, 452, 459, 704, 792, 1198, 1354, 1421, 1472
BceAI ACGGC 2 cut(s) 1370, 1622
BciT130I CCWGG 5 cut(s) 667, 1220, 1647, 1664, 2117
BclI TGATCA 2 cut(s) 1564, 1708
BcnI CCSGG 1 cut(s) 1011
BcoDI GTCTC 3 cut(s) 164, 986, 1445
BfaI CTAG 4 cut(s) 1230, 1806, 1836, 2417
BfmI CTRYAG 7 cut(s) 23, 189, 567, 678, 1074, 1170, 2009
BfuAI ACCTGC 3 cut(s) 1067, 2002, 2358
BglII AGATCT 3 cut(s) 375, 1951, 2143
BisI GCNGC 6 cut(s) 23, 1173, 1886, 2075, 2162, 2329
BlsI GCNGC 6 cut(s) 24, 1174, 1887, 2076, 2163, 2330
Bme1390I CCNGG 6 cut(s) 667, 1011, 1220, 1647, 1664, 2117
Bme18I GGWCC 2 cut(s) 1007, 1730
BmeRI GACNNNNNGTC 1 cut(s) 92
BmgT120I GGNCC 4 cut(s) 1007, 1557, 1730, 2252
BmiI GGNNCC 4 cut(s) 355, 765, 1008, 1732
BmrFI CCNGG 6 cut(s) 667, 1011, 1220, 1647, 1664, 2117
BmrI ACTGGG 2 cut(s) 2249, 2369
BmsI GCATC 8 cut(s) 389, 411, 836, 1242, 1806, 1947, 1960, 2214
BmuI ACTGGG 2 cut(s) 2249, 2369
BpmI CTGGAG 4 cut(s) 246, 281, 2138, 2268
Bpu10I CCTNAGC 2 cut(s) 672, 927
BpuEI CTTGAG 1 cut(s) 605
BpuMI CCSGG 1 cut(s) 1011
BsaBI GATNNNNATC 1 cut(s) 576
BsaI GGTCTC 1 cut(s) 1445
BsaJI CCNNGG 4 cut(s) 357, 427, 1218, 1662
BsaXI ACNNNNNCTCC 6 cut(s) 864, 894, 1623, 1653, 2213, 2243
Bsc4I CCNNNNNNNGG 2 cut(s) 1175, 2291
Bse3DI GCAATG 1 cut(s) 1708
Bse8I GATNNNNATC 1 cut(s) 576
BseBI CCWGG 5 cut(s) 667, 1220, 1647, 1664, 2117
BseDI CCNNGG 4 cut(s) 357, 427, 1218, 1662
BseGI GGATG 9 cut(s) 325, 402, 404, 577, 742, 803, 851, 892, 1432
BseJI GATNNNNATC 1 cut(s) 576
BseLI CCNNNNNNNGG 2 cut(s) 1175, 2291
BseMI GCAATG 1 cut(s) 1708
BseMII CTCAG 4 cut(s) 663, 918, 1336, 1456
BseXI GCAGC 6 cut(s) 9, 1184, 1897, 2061, 2148, 2315
BseYI CCCAGC 1 cut(s) 518
BsgI GTGCAG 1 cut(s) 1452
Bsh1236I CGCG 1 cut(s) 690
Bsh1285I CGRYCG 1 cut(s) 2292
BshFI GGCC 5 cut(s) 670, 1385, 1487, 1558, 2253
BsiEI CGRYCG 1 cut(s) 2292
BsiHKAI GWGCWC 1 cut(s) 2416
BsiSI CCGG 1 cut(s) 1010
BslFI GGGAC 4 cut(s) 395, 1236, 1716, 1833
BslI CCNNNNNNNGG 2 cut(s) 1175, 2291
BsmAI GTCTC 3 cut(s) 164, 986, 1445
BsmFI GGGAC 4 cut(s) 395, 1236, 1716, 1833
BsmI GAATGC 5 cut(s) 40, 822, 1874, 1973, 2330
BsnI GGCC 5 cut(s) 670, 1385, 1487, 1558, 2253
Bso31I GGTCTC 1 cut(s) 1445
Bsp1286I GDGCHC 2 cut(s) 1613, 2416
Bsp1407I TGTACA 2 cut(s) 1022, 2344
Bsp19I CCATGG 1 cut(s) 357
BspACI CCGC 4 cut(s) 102, 884, 1996, 2288
BspANI GGCC 5 cut(s) 670, 1385, 1487, 1558, 2253
BspCNI CTCAG 4 cut(s) 664, 919, 1335, 1455
BspFNI CGCG 1 cut(s) 690
BspHI TCATGA 2 cut(s) 826, 1966
BspLI GGNNCC 4 cut(s) 355, 765, 1008, 1732
BspMAI CTGCAG 4 cut(s) 27, 1078, 1174, 2013
BspMI ACCTGC 3 cut(s) 1067, 2002, 2358
BspPI GGATC 9 cut(s) 264, 294, 516, 642, 667, 1578, 1934, 1986, 2353
BspTNI GGTCTC 1 cut(s) 1445
BsrDI GCAATG 1 cut(s) 1708
BsrGI TGTACA 2 cut(s) 1022, 2344
BssECI CCNNGG 4 cut(s) 357, 427, 1218, 1662
BssT1I CCWWGG 2 cut(s) 357, 427
Bst2UI CCWGG 5 cut(s) 667, 1220, 1647, 1664, 2117
Bst4CI ACNGT 8 cut(s) 59, 622, 743, 941, 1380, 1462, 1774, 2265
Bst6I CTCTTC 3 cut(s) 1276, 1518, 1547
BstAPI GCANNNNNTGC 2 cut(s) 2337, 2411
BstAUI TGTACA 2 cut(s) 1022, 2344
BstC8I GCNNGC 6 cut(s) 12, 82, 1311, 2205, 2399, 2403
BstDEI CTNAG 6 cut(s) 672, 927, 1155, 1322, 1442, 1979
BstDSI CCRYGG 1 cut(s) 357
BstENI CCTNNNNNAGG 1 cut(s) 1173
BstF5I GGATG 9 cut(s) 325, 402, 404, 577, 742, 803, 851, 892, 1432
BstFNI CGCG 1 cut(s) 690
BstHHI GCGC 3 cut(s) 40, 692, 1020
BstMAI GTCTC 3 cut(s) 164, 986, 1445
BstMCI CGRYCG 1 cut(s) 2292
BstMWI GCNNNNNNNGC 8 cut(s) 11, 901, 2060, 2167, 2233, 2285, 2337, 2411
BstNI CCWGG 5 cut(s) 667, 1220, 1647, 1664, 2117
BstNSI RCATGY 2 cut(s) 1924, 2207
BstSCI CCNGG 6 cut(s) 665, 1009, 1218, 1645, 1662, 2115
BstSFI CTRYAG 7 cut(s) 23, 189, 567, 678, 1074, 1170, 2009
BstUI CGCG 1 cut(s) 690
BstV1I GCAGC 6 cut(s) 9, 1184, 1897, 2061, 2148, 2315
BstXI CCANNNNNNTGG 1 cut(s) 710
BsuRI GGCC 5 cut(s) 670, 1385, 1487, 1558, 2253
BtgI CCRYGG 1 cut(s) 357
BtsCI GGATG 9 cut(s) 325, 402, 404, 577, 742, 803, 851, 892, 1432
BtsI GCAGTG 1 cut(s) 1440
BtsIMutI CAGTG 7 cut(s) 96, 162, 1156, 1440, 1547, 2065, 2262
BveI ACCTGC 3 cut(s) 1067, 2002, 2358
Cac8I GCNNGC 6 cut(s) 12, 82, 1311, 2205, 2399, 2403
CciI TCATGA 2 cut(s) 826, 1966
CfoI GCGC 3 cut(s) 40, 692, 1020
Cfr13I GGNCC 4 cut(s) 1007, 1557, 1730, 2252
Csp6I GTAC 8 cut(s) 686, 1023, 1238, 1643, 1770, 1983, 2090, 2345
CviQI GTAC 8 cut(s) 686, 1023, 1238, 1643, 1770, 1983, 2090, 2345
DdeI CTNAG 6 cut(s) 672, 927, 1155, 1322, 1442, 1979
DraI TTTAAA 2 cut(s) 541, 1140
DriI GACNNNNNGTC 1 cut(s) 92
EaeI YGGCCR 1 cut(s) 1383
Eam1104I CTCTTC 3 cut(s) 1276, 1518, 1547
Eam1105I GACNNNNNGTC 1 cut(s) 92
EarI CTCTTC 3 cut(s) 1276, 1518, 1547
Eco130I CCWWGG 2 cut(s) 357, 427
Eco147I AGGCCT 2 cut(s) 670, 1487
Eco24I GRGCYC 1 cut(s) 1613
Eco31I GGTCTC 1 cut(s) 1445
Eco47I GGWCC 2 cut(s) 1007, 1730
Eco57I CTGAAG 5 cut(s) 392, 1571, 1803, 1968, 2288
EcoNI CCTNNNNNAGG 1 cut(s) 1173
EcoO109I RGGNCCY 1 cut(s) 1557
EcoRII CCWGG 5 cut(s) 665, 1218, 1645, 1662, 2115
EcoT14I CCWWGG 2 cut(s) 357, 427
EcoT22I ATGCAT 3 cut(s) 404, 824, 1975
EcoT38I GRGCYC 1 cut(s) 1613
ErhI CCWWGG 2 cut(s) 357, 427
FaqI GGGAC 4 cut(s) 395, 1236, 1716, 1833
FauI CCCGC 1 cut(s) 2003
FauNDI CATATG 2 cut(s) 907, 1841
FbaI TGATCA 2 cut(s) 1564, 1708
FblI GTMKAC 1 cut(s) 2267
Fnu4HI GCNGC 6 cut(s) 23, 1173, 1886, 2075, 2162, 2329
FokI GGATG 9 cut(s) 312, 389, 411, 564, 749, 810, 858, 899, 1439
FriOI GRGCYC 1 cut(s) 1613
Fsp4HI GCNGC 6 cut(s) 23, 1173, 1886, 2075, 2162, 2329
FspBI CTAG 4 cut(s) 1230, 1806, 1836, 2417
GlaI GCGC 3 cut(s) 39, 691, 1019
GluI GCNGC 6 cut(s) 23, 1173, 1886, 2075, 2162, 2329
GsaI CCCAGC 1 cut(s) 522
GsuI CTGGAG 4 cut(s) 246, 281, 2138, 2268
HaeIII GGCC 5 cut(s) 670, 1385, 1487, 1558, 2253
HapII CCGG 1 cut(s) 1010
HhaI GCGC 3 cut(s) 40, 692, 1020
Hin6I GCGC 3 cut(s) 38, 690, 1018
HinP1I GCGC 3 cut(s) 38, 690, 1018
HincII GTYRAC 1 cut(s) 1363
HindII GTYRAC 1 cut(s) 1363
HindIII AAGCTT 1 cut(s) 2234
HpaII CCGG 1 cut(s) 1010
HphI GGTGA 4 cut(s) 116, 347, 1870, 2029
Hpy166II GTNNAC 4 cut(s) 450, 1363, 2268, 2347
Hpy8I GTNNAC 4 cut(s) 450, 1363, 2268, 2347
HpyAV CCTTC 7 cut(s) 388, 545, 742, 979, 1644, 1997, 2383
HpyCH4III ACNGT 8 cut(s) 59, 622, 743, 941, 1380, 1462, 1774, 2265
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 901, 2060, 2167, 2233, 2285, 2337, 2411
HpyF3I CTNAG 6 cut(s) 672, 927, 1155, 1322, 1442, 1979
HspAI GCGC 3 cut(s) 38, 690, 1018
Ksp22I TGATCA 2 cut(s) 1564, 1708
LmnI GCTCC 4 cut(s) 227, 763, 911, 1188
Lsp1109I GCAGC 6 cut(s) 9, 1184, 1897, 2061, 2148, 2315
LweI GCATC 8 cut(s) 389, 411, 836, 1242, 1806, 1947, 1960, 2214
MaeI CTAG 4 cut(s) 1230, 1806, 1836, 2417
MaeIII GTNAC 3 cut(s) 94, 104, 1090
MfeI CAATTG 1 cut(s) 132
MhlI GDGCHC 2 cut(s) 1613, 2416
MlyI GAGTC 4 cut(s) 166, 882, 1648, 2304
MmeI TCCRAC 1 cut(s) 2272
Mph1103I ATGCAT 3 cut(s) 404, 824, 1975
MroXI GAANNNNTTC 1 cut(s) 1108
MslI CAYNNNNRTG 2 cut(s) 708, 2208
MspA1I CMGCKG 3 cut(s) 306, 314, 2288
MspI CCGG 1 cut(s) 1010
MspR9I CCNGG 6 cut(s) 667, 1011, 1220, 1647, 1664, 2117
MunI CAATTG 1 cut(s) 132
Mva1269I GAATGC 5 cut(s) 40, 822, 1874, 1973, 2330
MvaI CCWGG 5 cut(s) 667, 1220, 1647, 1664, 2117
MvnI CGCG 1 cut(s) 690
MwoI GCNNNNNNNGC 8 cut(s) 11, 901, 2060, 2167, 2233, 2285, 2337, 2411
NciI CCSGG 1 cut(s) 1011
NcoI CCATGG 1 cut(s) 357
NdeI CATATG 2 cut(s) 907, 1841
NlaIV GGNNCC 4 cut(s) 355, 765, 1008, 1732
NmuCI GTSAC 2 cut(s) 94, 104
NsiI ATGCAT 3 cut(s) 404, 824, 1975
NspI RCATGY 2 cut(s) 1924, 2207
PaeI GCATGC 1 cut(s) 2207
PagI TCATGA 2 cut(s) 826, 1966
PaqCI CACCTGC 3 cut(s) 1067, 2002, 2358
PceI AGGCCT 2 cut(s) 670, 1487
PciI ACATGT 1 cut(s) 1920
PctI GAATGC 5 cut(s) 40, 822, 1874, 1973, 2330
PdmI GAANNNNTTC 1 cut(s) 1108
PfeI GAWTC 9 cut(s) 185, 782, 975, 1070, 1165, 1265, 1286, 1391, 2425
PfoI TCCNGGA 1 cut(s) 2115
PkrI GCNGC 6 cut(s) 24, 1174, 1887, 2076, 2163, 2330
PleI GAGTC 4 cut(s) 165, 881, 1648, 2303
PpsI GAGTC 4 cut(s) 165, 881, 1648, 2303
PscI ACATGT 1 cut(s) 1920
PshBI ATTAAT 1 cut(s) 1107
Psp6I CCWGG 5 cut(s) 665, 1218, 1645, 1662, 2115
PspFI CCCAGC 1 cut(s) 518
PspGI CCWGG 5 cut(s) 665, 1218, 1645, 1662, 2115
PspN4I GGNNCC 4 cut(s) 355, 765, 1008, 1732
PspPI GGNCC 4 cut(s) 1007, 1557, 1730, 2252
PstI CTGCAG 4 cut(s) 27, 1078, 1174, 2013
PvuII CAGCTG 2 cut(s) 306, 314
RsaI GTAC 8 cut(s) 687, 1024, 1239, 1644, 1771, 1984, 2091, 2346
RsaNI GTAC 8 cut(s) 686, 1023, 1238, 1643, 1770, 1983, 2090, 2345
RseI CAYNNNNRTG 2 cut(s) 708, 2208
SatI GCNGC 6 cut(s) 23, 1173, 1886, 2075, 2162, 2329
Sau96I GGNCC 4 cut(s) 1007, 1557, 1730, 2252
SchI GAGTC 4 cut(s) 166, 882, 1648, 2304
ScrFI CCNGG 6 cut(s) 667, 1011, 1220, 1647, 1664, 2117
SduI GDGCHC 2 cut(s) 1613, 2416
SfaNI GCATC 8 cut(s) 389, 411, 836, 1242, 1806, 1947, 1960, 2214
SfcI CTRYAG 7 cut(s) 23, 189, 567, 678, 1074, 1170, 2009
SinI GGWCC 2 cut(s) 1007, 1730
SmiMI CAYNNNNRTG 2 cut(s) 708, 2208
SmlI CTYRAG 1 cut(s) 584
SmoI CTYRAG 1 cut(s) 584
SphI GCATGC 1 cut(s) 2207
SseBI AGGCCT 2 cut(s) 670, 1487
SsiI CCGC 4 cut(s) 102, 884, 1996, 2288
SspI AATATT 4 cut(s) 52, 67, 442, 723
SspMI CTAG 4 cut(s) 1230, 1806, 1836, 2417
StuI AGGCCT 2 cut(s) 670, 1487
StyD4I CCNGG 6 cut(s) 665, 1009, 1218, 1645, 1662, 2115
StyI CCWWGG 2 cut(s) 357, 427
TaaI ACNGT 8 cut(s) 59, 622, 743, 941, 1380, 1462, 1774, 2265
TaqI TCGA 4 cut(s) 463, 859, 1243, 1990
TaqII GACCGA 1 cut(s) 552
TatI WGTACW 3 cut(s) 1022, 1769, 2344
TfiI GAWTC 9 cut(s) 185, 782, 975, 1070, 1165, 1265, 1286, 1391, 2425
TscAI CASTG 7 cut(s) 103, 169, 1156, 1440, 1554, 2065, 2262
TseFI GTSAC 2 cut(s) 94, 104
TseI GCWGC 6 cut(s) 22, 1172, 1885, 2074, 2161, 2328
Tsp45I GTSAC 2 cut(s) 94, 104
TspDTI ATGAA 7 cut(s) 314, 564, 566, 747, 815, 1212, 1875
TspRI CASTG 7 cut(s) 103, 169, 1156, 1440, 1554, 2065, 2262
VpaK11BI GGWCC 2 cut(s) 1007, 1730
VspI ATTAAT 1 cut(s) 1107
XagI CCTNNNNNAGG 1 cut(s) 1173
XapI RAATTY 7 cut(s) 320, 542, 626, 862, 1529, 1905, 2367
XbaI TCTAGA 1 cut(s) 2416
XceI RCATGY 2 cut(s) 1924, 2207
XmiI GTMKAC 1 cut(s) 2267
XmnI GAANNNNTTC 1 cut(s) 1108
XspI CTAG 4 cut(s) 1230, 1806, 1836, 2417
Zsp2I ATGCAT 3 cut(s) 404, 824, 1975
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.