Rh5BG425500

May participate in a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001097
Physical Location & Seq
Reverse (-)
68909414 .. 68933150
23737 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG425500.1

Sequence Viewer

Length: 528 bp
ATGGAAACCAGTGCTAAGAAGCAGATTCCTATAGCAGGGTTGCTCCCAAAGTCTAATGAAAAGACGGTCAATAAATCCCTGGTTTCACCTAGCATCGTACCTCGAAGCAGTCCCAATTCAAAAGATTCAGCTAACTTTGGAAGAGAATCTGTTGTATTTCCGAAAAGGCGTGCTATGTTGCTCAGACCAGCACATGTGCGAAGCCCATCAAATAGCAAAGTTGACGTGGAGAAGCTACCGTTGGCCGTTGAATCTGAAACTTTTAGCAGTATGACGAGTAAATTGGTTGGTGTAGTGGGTCTCAGTTCTCAAATCAAAACTGTATCAGAAGATAGTGATAGAGAGGCCTGTAAGGAAAAACATTCTGTAATCAAGAGTGCAGAAGAGAAGTTTGAAGCAATATTATCACTGAAGAGGCCCTCTGATCAGGAATCCAGAAATAGATCGCTCAATAACAGCAAGGAAATGAGCCCCGTTAAAGTTGTCAATGGAGTTGCCGTTGTACCAGGAAGGACTCACCCTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Katanin_con80 WD40

Protein Analysis

175

Amino Acids

18.99

Weight (kDa)

9.91

Isoelectric Point (pI)

66.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 243
AcuI CTGAAG 1 cut(s) 431
AfaI GTAC 2 cut(s) 99, 504
AfiI CCNNNNNNNGG 1 cut(s) 35
AflIII ACRYGT 1 cut(s) 193
AgsI TTSAA 3 cut(s) 120, 251, 395
AjiI CACGTC 1 cut(s) 226
AjnI CCWGG 3 cut(s) 78, 505, 520
AjuI GAANNNNNNNTTGG 2 cut(s) 224, 256
AluBI AGCT 2 cut(s) 131, 235
AluI AGCT 2 cut(s) 131, 235
Alw26I GTCTC 1 cut(s) 305
AoxI GGCC 3 cut(s) 243, 345, 416
AspS9I GGNCC 1 cut(s) 417
AsuHPI GGTGA 2 cut(s) 78, 509
BanII GRGCYC 1 cut(s) 473
BccI CCATC 1 cut(s) 214
BceAI ACGGC 2 cut(s) 230, 482
BciT130I CCWGG 3 cut(s) 80, 507, 522
BclI TGATCA 1 cut(s) 424
BcoDI GTCTC 1 cut(s) 305
BfaI CTAG 1 cut(s) 90
BfmI CTRYAG 1 cut(s) 30
Bme1390I CCNGG 3 cut(s) 80, 507, 522
BmgBI CACGTC 1 cut(s) 226
BmgT120I GGNCC 1 cut(s) 417
BmrFI CCNGG 3 cut(s) 80, 507, 522
BmsI GCATC 1 cut(s) 102
BsaI GGTCTC 1 cut(s) 305
BsaJI CCNNGG 2 cut(s) 78, 520
BsaXI ACNNNNNCTCC 2 cut(s) 483, 513
Bsc4I CCNNNNNNNGG 1 cut(s) 35
Bse1I ACTGG 1 cut(s) 9
BseBI CCWGG 3 cut(s) 80, 507, 522
BseDI CCNNGG 2 cut(s) 78, 520
BseLI CCNNNNNNNGG 1 cut(s) 35
BseMII CTCAG 2 cut(s) 196, 316
BseNI ACTGG 1 cut(s) 9
BsgI GTGCAG 1 cut(s) 399
BshFI GGCC 3 cut(s) 245, 347, 418
BslFI GGGAC 1 cut(s) 96
BslI CCNNNNNNNGG 1 cut(s) 35
BsmAI GTCTC 1 cut(s) 305
BsmFI GGGAC 1 cut(s) 96
BsnI GGCC 3 cut(s) 245, 347, 418
Bso31I GGTCTC 1 cut(s) 305
Bsp1286I GDGCHC 1 cut(s) 473
Bsp143I GATC 2 cut(s) 424, 443
BspANI GGCC 3 cut(s) 245, 347, 418
BspCNI CTCAG 2 cut(s) 195, 315
BspTNI GGTCTC 1 cut(s) 305
BsrI ACTGG 1 cut(s) 9
BssECI CCNNGG 2 cut(s) 78, 520
BssMI GATC 2 cut(s) 424, 443
Bst2UI CCWGG 3 cut(s) 80, 507, 522
Bst4CI ACNGT 3 cut(s) 67, 240, 322
Bst6I CTCTTC 3 cut(s) 136, 378, 407
BstC8I GCNNGC 1 cut(s) 171
BstDEI CTNAG 3 cut(s) 15, 182, 302
BstENI CCTNNNNNAGG 1 cut(s) 33
BstKTI GATC 2 cut(s) 427, 446
BstMAI GTCTC 1 cut(s) 305
BstMBI GATC 2 cut(s) 424, 443
BstNI CCWGG 3 cut(s) 80, 507, 522
BstNSI RCATGY 1 cut(s) 197
BstSCI CCNGG 3 cut(s) 78, 505, 520
BstSFI CTRYAG 1 cut(s) 30
BsuRI GGCC 3 cut(s) 245, 347, 418
BtrI CACGTC 1 cut(s) 226
BtsIMutI CAGTG 2 cut(s) 16, 407
Cac8I GCNNGC 1 cut(s) 171
Cfr13I GGNCC 1 cut(s) 417
Csp6I GTAC 2 cut(s) 98, 503
CviAII CATG 1 cut(s) 194
CviJI RGCY 7 cut(s) 131, 204, 235, 245, 347, 418, 471
CviKI_1 RGCY 7 cut(s) 131, 204, 235, 245, 347, 418, 471
CviQI GTAC 2 cut(s) 98, 503
DdeI CTNAG 3 cut(s) 15, 182, 302
DpnI GATC 2 cut(s) 426, 445
DpnII GATC 2 cut(s) 424, 443
EaeI YGGCCR 1 cut(s) 243
Eam1104I CTCTTC 3 cut(s) 136, 378, 407
EarI CTCTTC 3 cut(s) 136, 378, 407
Eco147I AGGCCT 1 cut(s) 347
Eco24I GRGCYC 1 cut(s) 473
Eco31I GGTCTC 1 cut(s) 305
Eco57I CTGAAG 1 cut(s) 431
EcoNI CCTNNNNNAGG 1 cut(s) 33
EcoO109I RGGNCCY 1 cut(s) 417
EcoRII CCWGG 3 cut(s) 78, 505, 520
EcoT38I GRGCYC 1 cut(s) 473
FaeI CATG 1 cut(s) 197
FaiI YATR 4 cut(s) 32, 176, 195, 272
FaqI GGGAC 1 cut(s) 96
FatI CATG 1 cut(s) 193
FbaI TGATCA 1 cut(s) 424
FriOI GRGCYC 1 cut(s) 473
FspBI CTAG 1 cut(s) 90
HaeIII GGCC 3 cut(s) 245, 347, 418
Hin1II CATG 1 cut(s) 197
HincII GTYRAC 1 cut(s) 223
HindII GTYRAC 1 cut(s) 223
HinfI GANTC 6 cut(s) 25, 125, 146, 251, 431, 514
HphI GGTGA 2 cut(s) 78, 509
Hpy166II GTNNAC 1 cut(s) 223
Hpy188I TCNGA 5 cut(s) 162, 185, 256, 328, 424
Hpy188III TCNNGA 3 cut(s) 373, 428, 435
Hpy8I GTNNAC 1 cut(s) 223
HpyAV CCTTC 1 cut(s) 504
HpyCH4III ACNGT 3 cut(s) 67, 240, 322
HpyCH4IV ACGT 1 cut(s) 225
HpyCH4V TGCA 1 cut(s) 380
HpyF3I CTNAG 3 cut(s) 15, 182, 302
HpySE526I ACGT 1 cut(s) 225
Hsp92II CATG 1 cut(s) 197
Ksp22I TGATCA 1 cut(s) 424
Kzo9I GATC 2 cut(s) 424, 443
LmnI GCTCC 1 cut(s) 48
LweI GCATC 1 cut(s) 102
MaeI CTAG 1 cut(s) 90
MaeII ACGT 1 cut(s) 225
MalI GATC 2 cut(s) 426, 445
MboI GATC 2 cut(s) 424, 443
MboII GAAGA 4 cut(s) 153, 341, 395, 424
MhlI GDGCHC 1 cut(s) 473
MluCI AATT 2 cut(s) 115, 281
MlyI GAGTC 1 cut(s) 508
MnlI CCTC 4 cut(s) 111, 337, 408, 430
MseI TTAA 1 cut(s) 477
MspR9I CCNGG 3 cut(s) 80, 507, 522
MvaI CCWGG 3 cut(s) 80, 507, 522
NdeII GATC 2 cut(s) 424, 443
NlaIII CATG 1 cut(s) 197
NspI RCATGY 1 cut(s) 197
PceI AGGCCT 1 cut(s) 347
PciI ACATGT 1 cut(s) 193
PfeI GAWTC 5 cut(s) 25, 125, 146, 251, 431
PleI GAGTC 1 cut(s) 508
PpsI GAGTC 1 cut(s) 508
PscI ACATGT 1 cut(s) 193
Psp6I CCWGG 3 cut(s) 78, 505, 520
PspGI CCWGG 3 cut(s) 78, 505, 520
PspPI GGNCC 1 cut(s) 417
RsaI GTAC 2 cut(s) 99, 504
RsaNI GTAC 2 cut(s) 98, 503
SaqAI TTAA 1 cut(s) 477
Sau3AI GATC 2 cut(s) 424, 443
Sau96I GGNCC 1 cut(s) 417
SchI GAGTC 1 cut(s) 508
ScrFI CCNGG 3 cut(s) 80, 507, 522
SduI GDGCHC 1 cut(s) 473
SetI ASST 5 cut(s) 91, 103, 133, 228, 237
SfaNI GCATC 1 cut(s) 102
SfcI CTRYAG 1 cut(s) 30
Sse9I AATT 2 cut(s) 115, 281
SseBI AGGCCT 1 cut(s) 347
SspI AATATT 1 cut(s) 402
SspMI CTAG 1 cut(s) 90
StuI AGGCCT 1 cut(s) 347
StyD4I CCNGG 3 cut(s) 78, 505, 520
TaaI ACNGT 3 cut(s) 67, 240, 322
TaiI ACGT 1 cut(s) 228
TaqI TCGA 1 cut(s) 103
TasI AATT 2 cut(s) 115, 281
TfiI GAWTC 5 cut(s) 25, 125, 146, 251, 431
Tru1I TTAA 1 cut(s) 477
Tru9I TTAA 1 cut(s) 477
TscAI CASTG 2 cut(s) 16, 414
TspDTI ATGAA 1 cut(s) 72
TspRI CASTG 2 cut(s) 16, 414
XagI CCTNNNNNAGG 1 cut(s) 33
XceI RCATGY 1 cut(s) 197
XspI CTAG 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.