Rh5CG062400

May participate in a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0001097
Physical Location & Seq
Reverse (-)
4694175 .. 4701843
7669 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG062400.1

Sequence Viewer

Length: 2043 bp
ATGGCGAAGCGTGCATACAAGCTGCAGGAGTTTGTGGCGCATTCTGACAATATTAACTGTCTAAATATTGGGAAAAAGGCGTGCCGACTTTATGTCACCGGTGGTGATGATCACAAAGTCAATCTATGGACAATTGGCAAACCTACTGCGTTAATGAGTCTCACTGGTCATACAAGTCCAGTTGAATCTGTAGCTTTTAATTCAGCAGAAGTTTTGGTGCTTGCTGGAGCTTCTACTGGTGCAACAAAAATATGGGATCTGGAGGAAACAAAGAGAGTACGCGCAATTACTTTCCACCCTGATGGGAGGACAATATTTTCTGGATTGGATGACAGTTTGAAGGTTTATTCATGGGAGCCTGTAATATCCCATGATTCTGTTGATATGGGATGGTCAAGACTTGCTGACTTATGCATTCATGACGGGAAGCTATTGGGATGCTCTTACTATCGAAATTCTATTGGAGTCTGGGTTGCGGATGTGTCGCTTATTAAGCCATATGGAGCTGGTTCAACACCTGAGCAAAATGACTGTTTAGATGAGAAATCTCATGTAAAGTTGCAAGGAATCATGGAGAAGGTAGAGACTGGTGTAAGGGGTCCGGGTTTCCGCTGTACGTCTCCTGATTATGACACAAAGGAAATAAAGAACATATATGTTGATTCTGCAGGTGGAAAGCCAGTTACCTCACAGAGAATTAATTCTTCAAAAGTTTTACCCCCATTGGATTTAAAGGAAATCAGTGCTAAGAAGCAGATTCCTGCAGCAGGGTCGCTCCCAAAGTCTATTGAAAAGACGGTCAATAAATCCCTGGTTGTGCCTAGCATCGTACCTCGAAGCAGTCCCAATGCAAAGGATTCAGCTAACTCTGGAAGAGAATCTGTTGGATTTTCGAAAACTAGGCGTGGCATGTTGCTCAGACCAGCTCATGTGCGAAACGCATCAAATAGCAAACTTGACGTGGAGAAGCTACCGTTGGCCGTTGAATCTGAAACTTTCAGCAGTATGACGAGTAAGTTGGATGGTGCAGTGGGTCTCAGTTCTCAAATCAAAACTGTATCAGAAGATAGTGATAGAGAGGACTGTAAGGAAAAACATTCTGCAATCAAGAGTGTAGAAGAGAAATTTGAAGCAGTATTATCACTGAAGAGACCCTCTGATCAAGAAACCAGAAATGGATCCCTCAATAACAGCAAGGAAGTGAGCCCCATTAAAGTTGTCAATGGAGTTGCCGTTGTACCAGGAAGGACTCGCACCCTGGTTGAGAGATTTGAAAGGAGAGAGAGATTTAGTAGCAATGACGATCAAGCAACTGGTGTATTTGGTCCCGTGATACCTGAATATCAAGCAACCAGCACATCTCGTACTGTGATACCTGAAGATCAAGCAAGTAGCATATCTAGTCCTGTGATGCCTGAAGATCAAGCAACTAGCATATGTAGTCCCGTGATACATGAAATGGATAAAACATTCTCCATGCAGCGAGAAGAAGAACCTCAAATTTCTGGGAGGCACATGTCATCTGCAAACAATGATCCTGCTGAAGATCTGATGCAAACTCATGATGCATTCCTAAGTACCCTTCGATCCCGCCTGACAAAACTGCAGGTGGTGAGGCATTTTTGGGAACGAAATGATATTAAAGGTGCTATCAGTGCTATCAGAAAGCTGCCAGATCATTCGGTACAAGCAGATGTGATTAGTGTTCTCCTGGAGAAAATGGATATTCTGACATTAGATCTCTTTTCTTGTTTGCTGCCTGTGCTTCTGGGCTTACTTGATAGCAAAATTGAGAGGCATGCAAATGTGTCATTGGAGATGCTGTTGAAGCTAGTAGCAGTCTTTGGCCCAGTGATACGGTCTACCATTTCAGCACCTCCAGCGGTCGGAGTCAATCTTCAGGCAGAGCAAAGGCTGGAGTGCTGCAACCAATGCTCTGTACACCTGCAAAAGATCCAGAAAATTCTCCCAGTCTTTGTAAGGAAGGGGGGCGTGCTGGCAAGATGTGCTCTAGAATTGAATCTAATTCTTCAAGAGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Katanin_con80 WD40

Protein Analysis

680

Amino Acids

74.56

Weight (kDa)

7.88

Isoelectric Point (pI)

48.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 7 - 97 1.1e-08 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 8 - 101 3.7e-13 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 8 - 96 2.7e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 9 - 99 2.7e-11 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 15 - 157 8e-11 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 16 - 120 3e-10 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 29 - 118 5e-06 Echinoderm microtubule-associated protein second beta-propeller
Katanin_con80 PF13925 519 - 676 2.7e-53 con80 domain of Katanin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 659, 1597, 1953
Acc36I ACCTGC 3 cut(s) 659, 1597, 1953
AccI GTMKAC 1 cut(s) 1862
AccII CGCG 1 cut(s) 282
AciI CCGC 4 cut(s) 476, 610, 1591, 1883
AclWI GGATC 6 cut(s) 264, 1173, 1186, 1529, 1581, 1948
AcoI YGGCCR 1 cut(s) 978
AcsI RAATTY 4 cut(s) 454, 1124, 1500, 1962
AcuI CTGAAG 5 cut(s) 1166, 1398, 1437, 1563, 1883
AfaI GTAC 8 cut(s) 279, 616, 831, 1239, 1366, 1579, 1686, 1941
AfiI CCNNNNNNNGG 2 cut(s) 767, 1886
AflIII ACRYGT 1 cut(s) 1515
AgeI ACCGGT 1 cut(s) 98
AhdI GACNNNNNGTC 1 cut(s) 92
AjiI CACGTC 1 cut(s) 961
AjnI CCWGG 4 cut(s) 810, 1240, 1257, 1710
AjuI GAANNNNNNNTTGG 2 cut(s) 959, 991
Alw21I GWGCWC 1 cut(s) 2011
Alw26I GTCTC 5 cut(s) 164, 578, 624, 1040, 1144
AlwI GGATC 6 cut(s) 264, 1173, 1186, 1529, 1581, 1948
AoxI GGCC 2 cut(s) 978, 1846
ApeKI GCWGC 6 cut(s) 22, 764, 1480, 1669, 1756, 1923
ApoI RAATTY 4 cut(s) 454, 1124, 1500, 1962
AseI ATTAAT 1 cut(s) 699
AsiGI ACCGGT 1 cut(s) 98
Asp700I GAANNNNTTC 1 cut(s) 700
AspLEI GCGC 2 cut(s) 40, 284
AspS9I GGNCC 3 cut(s) 599, 1325, 1847
AsuC2I CCSGG 1 cut(s) 603
AsuHPI GGTGA 3 cut(s) 88, 116, 1624
AsuII TTCGAA 1 cut(s) 893
AvaII GGWCC 2 cut(s) 599, 1325
BamHI GGATCC 1 cut(s) 1178
BanII GRGCYC 1 cut(s) 1208
BarI GAAGNNNNNNTAC 1 cut(s) 31
Bbv12I GWGCWC 1 cut(s) 2011
BbvI GCAGC 6 cut(s) 9, 776, 1492, 1656, 1743, 1910
BccI CCATC 3 cut(s) 296, 384, 1016
BceAI ACGGC 2 cut(s) 965, 1217
BcgI CGANNNNNNTGC 2 cut(s) 753, 787
BciT130I CCWGG 4 cut(s) 812, 1242, 1259, 1712
BclI TGATCA 2 cut(s) 109, 1159
BcnI CCSGG 1 cut(s) 603
BcoDI GTCTC 5 cut(s) 164, 578, 624, 1040, 1144
BfaI CTAG 6 cut(s) 822, 900, 1401, 1431, 1832, 2012
BfmI CTRYAG 5 cut(s) 23, 189, 666, 762, 1604
BfuAI ACCTGC 3 cut(s) 659, 1597, 1953
BglII AGATCT 2 cut(s) 1546, 1738
BisI GCNGC 6 cut(s) 23, 765, 1481, 1670, 1757, 1924
BlsI GCNGC 6 cut(s) 24, 766, 1482, 1671, 1758, 1925
Bme1390I CCNGG 5 cut(s) 603, 812, 1242, 1259, 1712
Bme18I GGWCC 2 cut(s) 599, 1325
BmeRI GACNNNNNGTC 1 cut(s) 92
BmgBI CACGTC 1 cut(s) 961
BmgT120I GGNCC 3 cut(s) 599, 1325, 1847
BmiI GGNNCC 4 cut(s) 357, 600, 1180, 1327
BmrFI CCNGG 5 cut(s) 603, 812, 1242, 1259, 1712
BmrI ACTGGG 2 cut(s) 1844, 1964
BmsI GCATC 7 cut(s) 428, 834, 950, 1401, 1542, 1555, 1809
BmuI ACTGGG 2 cut(s) 1844, 1964
BpmI CTGGAG 5 cut(s) 246, 281, 1733, 1863, 1937
Bpu10I CCTNAGC 1 cut(s) 519
Bpu14I TTCGAA 1 cut(s) 893
BpuMI CCSGG 1 cut(s) 603
BsaI GGTCTC 2 cut(s) 1040, 1144
BsaJI CCNNGG 2 cut(s) 810, 1257
BsaWI WCCGGW 1 cut(s) 98
BsaXI ACNNNNNCTCC 6 cut(s) 456, 486, 1218, 1248, 1808, 1838
Bsc4I CCNNNNNNNGG 2 cut(s) 767, 1886
Bse118I RCCGGY 1 cut(s) 98
Bse1I ACTGG 8 cut(s) 169, 179, 241, 592, 680, 1318, 1850, 1970
Bse3DI GCAATG 1 cut(s) 1303
BseBI CCWGG 4 cut(s) 812, 1242, 1259, 1712
BseDI CCNNGG 2 cut(s) 810, 1257
BseGI GGATG 5 cut(s) 334, 395, 443, 484, 1027
BseLI CCNNNNNNNGG 2 cut(s) 767, 1886
BseMI GCAATG 1 cut(s) 1303
BseMII CTCAG 3 cut(s) 510, 931, 1051
BseNI ACTGG 8 cut(s) 169, 179, 241, 592, 680, 1318, 1850, 1970
BseXI GCAGC 6 cut(s) 9, 776, 1492, 1656, 1743, 1910
BsgI GTGCAG 1 cut(s) 1047
Bsh1236I CGCG 1 cut(s) 282
Bsh1285I CGRYCG 1 cut(s) 1887
BshFI GGCC 2 cut(s) 980, 1848
BshTI ACCGGT 1 cut(s) 98
BsiEI CGRYCG 1 cut(s) 1887
BsiHKAI GWGCWC 1 cut(s) 2011
BsiSI CCGG 2 cut(s) 99, 602
BslFI GGGAC 3 cut(s) 828, 1311, 1428
BslI CCNNNNNNNGG 2 cut(s) 767, 1886
BsmAI GTCTC 5 cut(s) 164, 578, 624, 1040, 1144
BsmBI CGTCTC 1 cut(s) 624
BsmFI GGGAC 3 cut(s) 828, 1311, 1428
BsmI GAATGC 3 cut(s) 40, 414, 1568
BsnI GGCC 2 cut(s) 980, 1848
Bso31I GGTCTC 2 cut(s) 1040, 1144
Bsp119I TTCGAA 1 cut(s) 893
Bsp1286I GDGCHC 2 cut(s) 1208, 2011
Bsp1407I TGTACA 1 cut(s) 1939
BspACI CCGC 4 cut(s) 476, 610, 1591, 1883
BspANI GGCC 2 cut(s) 980, 1848
BspCNI CTCAG 3 cut(s) 511, 930, 1050
BspFNI CGCG 1 cut(s) 282
BspHI TCATGA 2 cut(s) 418, 1561
BspLI GGNNCC 4 cut(s) 357, 600, 1180, 1327
BspMAI CTGCAG 4 cut(s) 27, 670, 766, 1608
BspMI ACCTGC 3 cut(s) 659, 1597, 1953
BspPI GGATC 6 cut(s) 264, 1173, 1186, 1529, 1581, 1948
BspT104I TTCGAA 1 cut(s) 893
BspTNI GGTCTC 2 cut(s) 1040, 1144
BsrDI GCAATG 1 cut(s) 1303
BsrFI RCCGGY 1 cut(s) 98
BsrGI TGTACA 1 cut(s) 1939
BsrI ACTGG 8 cut(s) 169, 179, 241, 592, 680, 1318, 1850, 1970
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 2 cut(s) 810, 1257
Bst2UI CCWGG 4 cut(s) 812, 1242, 1259, 1712
Bst4CI ACNGT 9 cut(s) 59, 335, 533, 799, 975, 1057, 1085, 1369, 1860
Bst6I CTCTTC 3 cut(s) 868, 1113, 1142
BstAPI GCANNNNNTGC 2 cut(s) 1932, 2006
BstAUI TGTACA 1 cut(s) 1939
BstBI TTCGAA 1 cut(s) 893
BstC8I GCNNGC 6 cut(s) 12, 82, 222, 1800, 1994, 1998
BstDEI CTNAG 5 cut(s) 519, 747, 917, 1037, 1574
BstENI CCTNNNNNAGG 1 cut(s) 765
BstF5I GGATG 5 cut(s) 334, 395, 443, 484, 1027
BstFNI CGCG 1 cut(s) 282
BstHHI GCGC 2 cut(s) 40, 284
BstMAI GTCTC 5 cut(s) 164, 578, 624, 1040, 1144
BstMCI CGRYCG 1 cut(s) 1887
BstMWI GCNNNNNNNGC 8 cut(s) 11, 493, 1655, 1762, 1828, 1880, 1932, 2006
BstNI CCWGG 4 cut(s) 812, 1242, 1259, 1712
BstNSI RCATGY 3 cut(s) 913, 1519, 1802
BstSCI CCNGG 5 cut(s) 601, 810, 1240, 1257, 1710
BstSFI CTRYAG 5 cut(s) 23, 189, 666, 762, 1604
BstUI CGCG 1 cut(s) 282
BstV1I GCAGC 6 cut(s) 9, 776, 1492, 1656, 1743, 1910
BstX2I RGATCY 5 cut(s) 256, 1178, 1546, 1738, 1953
BstXI CCANNNNNNTGG 1 cut(s) 302
BstYI RGATCY 5 cut(s) 256, 1178, 1546, 1738, 1953
BsuRI GGCC 2 cut(s) 980, 1848
BtrI CACGTC 1 cut(s) 961
BtsCI GGATG 5 cut(s) 334, 395, 443, 484, 1027
BtsI GCAGTG 1 cut(s) 1035
BtsIMutI CAGTG 6 cut(s) 162, 748, 1035, 1142, 1660, 1857
BveI ACCTGC 3 cut(s) 659, 1597, 1953
Cac8I GCNNGC 6 cut(s) 12, 82, 222, 1800, 1994, 1998
CciI TCATGA 2 cut(s) 418, 1561
CfoI GCGC 2 cut(s) 40, 284
Cfr10I RCCGGY 1 cut(s) 98
Cfr13I GGNCC 3 cut(s) 599, 1325, 1847
Csp6I GTAC 8 cut(s) 278, 615, 830, 1238, 1365, 1578, 1685, 1940
CspAI ACCGGT 1 cut(s) 98
CviQI GTAC 8 cut(s) 278, 615, 830, 1238, 1365, 1578, 1685, 1940
DdeI CTNAG 5 cut(s) 519, 747, 917, 1037, 1574
DraI TTTAAA 1 cut(s) 732
DriI GACNNNNNGTC 1 cut(s) 92
EaeI YGGCCR 1 cut(s) 978
Eam1104I CTCTTC 3 cut(s) 868, 1113, 1142
Eam1105I GACNNNNNGTC 1 cut(s) 92
EarI CTCTTC 3 cut(s) 868, 1113, 1142
Eco24I GRGCYC 1 cut(s) 1208
Eco31I GGTCTC 2 cut(s) 1040, 1144
Eco47I GGWCC 2 cut(s) 599, 1325
Eco57I CTGAAG 5 cut(s) 1166, 1398, 1437, 1563, 1883
EcoNI CCTNNNNNAGG 1 cut(s) 765
EcoRII CCWGG 4 cut(s) 810, 1240, 1257, 1710
EcoT22I ATGCAT 2 cut(s) 416, 1570
EcoT38I GRGCYC 1 cut(s) 1208
Esp3I CGTCTC 1 cut(s) 624
FaqI GGGAC 3 cut(s) 828, 1311, 1428
FauI CCCGC 1 cut(s) 1598
FauNDI CATATG 2 cut(s) 499, 1436
FbaI TGATCA 2 cut(s) 109, 1159
FblI GTMKAC 1 cut(s) 1862
Fnu4HI GCNGC 6 cut(s) 23, 765, 1481, 1670, 1757, 1924
FokI GGATG 5 cut(s) 341, 402, 450, 491, 1034
FriOI GRGCYC 1 cut(s) 1208
Fsp4HI GCNGC 6 cut(s) 23, 765, 1481, 1670, 1757, 1924
FspBI CTAG 6 cut(s) 822, 900, 1401, 1431, 1832, 2012
GlaI GCGC 2 cut(s) 39, 283
GluI GCNGC 6 cut(s) 23, 765, 1481, 1670, 1757, 1924
GsuI CTGGAG 5 cut(s) 246, 281, 1733, 1863, 1937
HaeIII GGCC 2 cut(s) 980, 1848
HapII CCGG 2 cut(s) 99, 602
HhaI GCGC 2 cut(s) 40, 284
Hin6I GCGC 2 cut(s) 38, 282
HinP1I GCGC 2 cut(s) 38, 282
HpaII CCGG 2 cut(s) 99, 602
HphI GGTGA 3 cut(s) 88, 116, 1624
Hpy166II GTNNAC 2 cut(s) 1863, 1942
Hpy188I TCNGA 9 cut(s) 46, 920, 991, 1063, 1159, 1551, 1664, 1731, 1889
Hpy8I GTNNAC 2 cut(s) 1863, 1942
HpyAV CCTTC 5 cut(s) 334, 571, 1239, 1592, 1978
HpyCH4III ACNGT 9 cut(s) 59, 335, 533, 799, 975, 1057, 1085, 1369, 1860
HpyCH4IV ACGT 2 cut(s) 617, 960
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 493, 1655, 1762, 1828, 1880, 1932, 2006
HpyF3I CTNAG 5 cut(s) 519, 747, 917, 1037, 1574
HpySE526I ACGT 2 cut(s) 617, 960
HspAI GCGC 2 cut(s) 38, 282
Ksp22I TGATCA 2 cut(s) 109, 1159
LmnI GCTCC 4 cut(s) 227, 355, 503, 780
Lsp1109I GCAGC 6 cut(s) 9, 776, 1492, 1656, 1743, 1910
LweI GCATC 7 cut(s) 428, 834, 950, 1401, 1542, 1555, 1809
MaeI CTAG 6 cut(s) 822, 900, 1401, 1431, 1832, 2012
MaeII ACGT 2 cut(s) 617, 960
MaeIII GTNAC 2 cut(s) 94, 682
MfeI CAATTG 1 cut(s) 132
MflI RGATCY 5 cut(s) 256, 1178, 1546, 1738, 1953
MhlI GDGCHC 2 cut(s) 1208, 2011
MlyI GAGTC 4 cut(s) 166, 474, 1243, 1899
MmeI TCCRAC 3 cut(s) 865, 999, 1867
Mph1103I ATGCAT 2 cut(s) 416, 1570
MroXI GAANNNNTTC 1 cut(s) 700
MseI TTAA 8 cut(s) 54, 152, 198, 492, 699, 731, 1212, 1641
MslI CAYNNNNRTG 2 cut(s) 300, 1803
MspA1I CMGCKG 2 cut(s) 612, 1883
MspI CCGG 2 cut(s) 99, 602
MspR9I CCNGG 5 cut(s) 603, 812, 1242, 1259, 1712
MunI CAATTG 1 cut(s) 132
Mva1269I GAATGC 3 cut(s) 40, 414, 1568
MvaI CCWGG 4 cut(s) 812, 1242, 1259, 1712
MvnI CGCG 1 cut(s) 282
MwoI GCNNNNNNNGC 8 cut(s) 11, 493, 1655, 1762, 1828, 1880, 1932, 2006
NciI CCSGG 1 cut(s) 603
NdeI CATATG 2 cut(s) 499, 1436
NlaIV GGNNCC 4 cut(s) 357, 600, 1180, 1327
NmuCI GTSAC 1 cut(s) 94
NsiI ATGCAT 2 cut(s) 416, 1570
NspI RCATGY 3 cut(s) 913, 1519, 1802
NspV TTCGAA 1 cut(s) 893
PaeI GCATGC 1 cut(s) 1802
PagI TCATGA 2 cut(s) 418, 1561
PaqCI CACCTGC 3 cut(s) 659, 1597, 1953
PciI ACATGT 1 cut(s) 1515
PctI GAATGC 3 cut(s) 40, 414, 1568
PdmI GAANNNNTTC 1 cut(s) 700
PfeI GAWTC 9 cut(s) 185, 374, 567, 662, 757, 857, 878, 986, 2020
PfoI TCCNGGA 1 cut(s) 1710
PinAI ACCGGT 1 cut(s) 98
PkrI GCNGC 6 cut(s) 24, 766, 1482, 1671, 1758, 1925
PleI GAGTC 4 cut(s) 165, 473, 1243, 1898
PpsI GAGTC 4 cut(s) 165, 473, 1243, 1898
PscI ACATGT 1 cut(s) 1515
PshBI ATTAAT 1 cut(s) 699
Psp6I CCWGG 4 cut(s) 810, 1240, 1257, 1710
PspGI CCWGG 4 cut(s) 810, 1240, 1257, 1710
PspN4I GGNNCC 4 cut(s) 357, 600, 1180, 1327
PspPI GGNCC 3 cut(s) 599, 1325, 1847
PstI CTGCAG 4 cut(s) 27, 670, 766, 1608
PsuI RGATCY 5 cut(s) 256, 1178, 1546, 1738, 1953
RsaI GTAC 8 cut(s) 279, 616, 831, 1239, 1366, 1579, 1686, 1941
RsaNI GTAC 8 cut(s) 278, 615, 830, 1238, 1365, 1578, 1685, 1940
RseI CAYNNNNRTG 2 cut(s) 300, 1803
SaqAI TTAA 8 cut(s) 54, 152, 198, 492, 699, 731, 1212, 1641
SatI GCNGC 6 cut(s) 23, 765, 1481, 1670, 1757, 1924
Sau96I GGNCC 3 cut(s) 599, 1325, 1847
SchI GAGTC 4 cut(s) 166, 474, 1243, 1899
ScrFI CCNGG 5 cut(s) 603, 812, 1242, 1259, 1712
SduI GDGCHC 2 cut(s) 1208, 2011
SfaNI GCATC 7 cut(s) 428, 834, 950, 1401, 1542, 1555, 1809
SfcI CTRYAG 5 cut(s) 23, 189, 666, 762, 1604
SfuI TTCGAA 1 cut(s) 893
SgrAI CRCCGGYG 1 cut(s) 98
SinI GGWCC 2 cut(s) 599, 1325
SmiMI CAYNNNNRTG 2 cut(s) 300, 1803
SphI GCATGC 1 cut(s) 1802
SsiI CCGC 4 cut(s) 476, 610, 1591, 1883
SspI AATATT 3 cut(s) 52, 67, 315
SspMI CTAG 6 cut(s) 822, 900, 1401, 1431, 1832, 2012
StyD4I CCNGG 5 cut(s) 601, 810, 1240, 1257, 1710
TaaI ACNGT 9 cut(s) 59, 335, 533, 799, 975, 1057, 1085, 1369, 1860
TaiI ACGT 2 cut(s) 620, 963
TaqI TCGA 4 cut(s) 451, 835, 893, 1585
TatI WGTACW 1 cut(s) 1939
TfiI GAWTC 9 cut(s) 185, 374, 567, 662, 757, 857, 878, 986, 2020
Tru1I TTAA 8 cut(s) 54, 152, 198, 492, 699, 731, 1212, 1641
Tru9I TTAA 8 cut(s) 54, 152, 198, 492, 699, 731, 1212, 1641
TscAI CASTG 6 cut(s) 169, 748, 1035, 1149, 1660, 1857
TseFI GTSAC 1 cut(s) 94
TseI GCWGC 6 cut(s) 22, 764, 1480, 1669, 1756, 1923
Tsp45I GTSAC 1 cut(s) 94
TspDTI ATGAA 3 cut(s) 339, 407, 1470
TspRI CASTG 6 cut(s) 169, 748, 1035, 1149, 1660, 1857
VpaK11BI GGWCC 2 cut(s) 599, 1325
VspI ATTAAT 1 cut(s) 699
XagI CCTNNNNNAGG 1 cut(s) 765
XapI RAATTY 4 cut(s) 454, 1124, 1500, 1962
XbaI TCTAGA 1 cut(s) 2011
XceI RCATGY 3 cut(s) 913, 1519, 1802
XmiI GTMKAC 1 cut(s) 1862
XmnI GAANNNNTTC 1 cut(s) 700
XspI CTAG 6 cut(s) 822, 900, 1401, 1431, 1832, 2012
Zsp2I ATGCAT 2 cut(s) 416, 1570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.