Rh5DG051700

May participate in a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001097
Physical Location & Seq
Reverse (-)
4354946 .. 4413818
58873 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG051700.1

Sequence Viewer

Length: 1761 bp
ATGGCGAAGCGTGCATACAAGCTGCAGGAGTTTGTGGCGCATTCTGACAATATTAACTGTCTAAATATTGGGAAAAAGGCGTGCCGACTTTATGTCACCGGTGGTGACGATCACAAAGTCAATCTATGGACAATTGGCAAACCTACTGCGTTAATGAGTCTCACTGGTCATACAAGTCCAGTTGAATCTGTGGCTTTTAATTCAGCAGAAGTTTTGGTGCTTGCTGGAGCTTCTACTGGTGCAACGAAAATATGGGATCTGGAGGAAACAAAGAGAGTACGCGCAATTACTTTCCACCCTGATGGGAGGACAATATTTTCTGGATTGGATGACAGTTTGAAGGTTTATTCATGGGAGCCTGTGATATCCCATGATTCTGTTGATATGGGATGGTCAAGACTTGCTGACTTATGCATTCATGACGGGAAGCTATTGGGATGCTCTTACTATCGAAATTCTATTGGAGTCTGGGTTGCGGATGTGTCGCTTATTAAGCCATATGGAGCTGGTTCAACACCTGAGCAAAATGACTGTTTAGATGAGAAATCTCATGTAAAGTTGCAAGGAATCATGGAGAAGGTAGAGACTGGTATAAGGGGGCCGGGTTTGCGCTGTATGTCTCCTGATTATGACACAAAGGAAATAAAGAACATATATATTGATTCTGCAGGTGGAAAGCCAGTTACCTCACAGAGAATTAATTCTTCAAAAGTTTTACCCCCATTGGATTTAAAGGAAACCAGTGCTAAGAAGCAGATTCCTGCAGCAGGGTCGCTCCCAAAGTCTAATGAAAAGATGGTCAATAAATCCCTGGTTGTGCCTAGCATCGTACCTCGAAGCAGTCCCAATGCAAAAGACTCAGCTAACTCTGGGAGAGATTCTGTTGCATTTTCGAAAACTAGGCGTGGTATGTTGGTCAGACCAGCTCATGTGCGAAGCCCATCAAATAGCAAAGTTGACATGGAGAAGCTACCGTTGGCCGTTGAATCTGAAACTTTCAGCAGTATGACGAGTAAATTGGATGGTGCAGTGGGTCTCGGTTCTCAAATCAAAACTGTATCAGAAGATAGTGATAGAGAGGCCTGTACGGAAAAACATTCTGCAATCAAGAGTGTAGAAGAGAAATTTGAAGCAGTATTATCACTGAAGAGGCCCTCTGATCAGGAAACCAGAAATGGATCCCTCAATAACAGCATGGTAGTGAGCCCCGTTAAAGTTGTCAATGGAGTTGCCGTTGTACCAGGAAGGACTCGCACCCTGGTTGAGAGATTTGAAAGGAGAGAAAGATTTAGTAGCAATGACGATCAAGCAACTGGCATATTTGGTCCCGTGATACCTGAATATCAAGCAACCAGCATATCTCGTACTGTGATACCTGAAGATCAAGCAAGTAGCATATCTAGTCCTGTGATGCCTGAAGATCAAGCAACTAGCATATGTAGTCCCGTGATACATGAAATGGATAAAGCATTCACCATGCAGCGAGAAGAAGAACCTCAAATTTCTGGGAGGCACATGTCATCTGCAAACAATGATCCTGCTGAAGATCTGATGCAAACTCATGATGCATTCCTAAGTACTCTTCAATCCAGCCTGACAAAACTGCAGGCATGCAAATGTGTCATTGGAGATGCTGTTGAAGCTAGTAGCAGTCTTTGGCACAGTGATACGGTCTACCATTTCAGCACCTCCAGCGGTCGGAGTCAATCTTCAGGCAGAGCAAAGGTATTACCACACACTCTTCTTGTAAGTCGTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Katanin_con80 WD40

Protein Analysis

586

Amino Acids

63.77

Weight (kDa)

6.95

Isoelectric Point (pI)

53.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 7 - 99 5e-09 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 8 - 101 2.9e-13 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 8 - 96 2.4e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 9 - 99 2.1e-11 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 15 - 157 6.3e-11 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 16 - 120 2.4e-10 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 29 - 118 4.8e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 659
Acc36I ACCTGC 1 cut(s) 659
AccI GTMKAC 1 cut(s) 1674
AccII CGCG 1 cut(s) 282
AciI CCGC 2 cut(s) 476, 1695
AclWI GGATC 4 cut(s) 264, 1173, 1186, 1529
AcoI YGGCCR 1 cut(s) 978
AcsI RAATTY 3 cut(s) 454, 1124, 1500
AcuI CTGAAG 5 cut(s) 1166, 1398, 1437, 1563, 1695
AfaI GTAC 6 cut(s) 279, 831, 1087, 1239, 1366, 1579
AfiI CCNNNNNNNGG 2 cut(s) 767, 1698
AflIII ACRYGT 1 cut(s) 1515
AgeI ACCGGT 1 cut(s) 98
AgsI TTSAA 9 cut(s) 185, 340, 513, 708, 986, 1130, 1274, 1586, 1640
AhdI GACNNNNNGTC 1 cut(s) 92
AjnI CCWGG 3 cut(s) 810, 1240, 1257
AjuI GAANNNNNNNTTGG 2 cut(s) 959, 991
AluBI AGCT 8 cut(s) 22, 230, 430, 506, 863, 926, 970, 1643
AluI AGCT 8 cut(s) 22, 230, 430, 506, 863, 926, 970, 1643
Alw26I GTCTC 4 cut(s) 164, 578, 624, 1040
AlwI GGATC 4 cut(s) 264, 1173, 1186, 1529
AoxI GGCC 4 cut(s) 599, 978, 1080, 1151
ApeKI GCWGC 3 cut(s) 22, 764, 1480
ApoI RAATTY 3 cut(s) 454, 1124, 1500
AseI ATTAAT 1 cut(s) 699
AsiGI ACCGGT 1 cut(s) 98
Asp700I GAANNNNTTC 1 cut(s) 700
AspLEI GCGC 3 cut(s) 40, 284, 612
AspS9I GGNCC 3 cut(s) 599, 1152, 1325
AsuC2I CCSGG 1 cut(s) 603
AsuHPI GGTGA 3 cut(s) 88, 116, 1465
AsuII TTCGAA 1 cut(s) 893
AvaII GGWCC 1 cut(s) 1325
BamHI GGATCC 1 cut(s) 1178
BanII GRGCYC 1 cut(s) 1208
BarI GAAGNNNNNNTAC 1 cut(s) 31
BbvI GCAGC 3 cut(s) 9, 776, 1492
BccI CCATC 5 cut(s) 296, 384, 790, 949, 1016
BceAI ACGGC 2 cut(s) 965, 1217
BcgI CGANNNNNNTGC 2 cut(s) 753, 787
BciT130I CCWGG 3 cut(s) 812, 1242, 1259
BclI TGATCA 1 cut(s) 1159
BcnI CCSGG 1 cut(s) 603
BcoDI GTCTC 4 cut(s) 164, 578, 624, 1040
BfaI CTAG 5 cut(s) 822, 900, 1401, 1431, 1644
BfmI CTRYAG 4 cut(s) 23, 666, 762, 1604
BfuAI ACCTGC 1 cut(s) 659
BglII AGATCT 1 cut(s) 1546
BisI GCNGC 3 cut(s) 23, 765, 1481
BlsI GCNGC 3 cut(s) 24, 766, 1482
BmcAI AGTACT 1 cut(s) 1579
Bme1390I CCNGG 4 cut(s) 603, 812, 1242, 1259
Bme18I GGWCC 1 cut(s) 1325
BmeRI GACNNNNNGTC 1 cut(s) 92
BmgT120I GGNCC 3 cut(s) 599, 1152, 1325
BmiI GGNNCC 4 cut(s) 357, 600, 1180, 1327
BmrFI CCNGG 4 cut(s) 603, 812, 1242, 1259
BmsI GCATC 6 cut(s) 428, 834, 1401, 1542, 1555, 1621
BpmI CTGGAG 3 cut(s) 246, 281, 1675
Bpu10I CCTNAGC 1 cut(s) 519
Bpu14I TTCGAA 1 cut(s) 893
BpuMI CCSGG 1 cut(s) 603
BsaI GGTCTC 1 cut(s) 1040
BsaJI CCNNGG 2 cut(s) 810, 1257
BsaWI WCCGGW 1 cut(s) 98
BsaXI ACNNNNNCTCC 6 cut(s) 456, 486, 1218, 1248, 1620, 1650
Bsc4I CCNNNNNNNGG 2 cut(s) 767, 1698
Bse118I RCCGGY 1 cut(s) 98
Bse1I ACTGG 7 cut(s) 169, 179, 241, 592, 680, 741, 1318
Bse3DI GCAATG 1 cut(s) 1303
BseBI CCWGG 3 cut(s) 812, 1242, 1259
BseDI CCNNGG 2 cut(s) 810, 1257
BseGI GGATG 5 cut(s) 334, 395, 443, 484, 1027
BseLI CCNNNNNNNGG 2 cut(s) 767, 1698
BseMI GCAATG 1 cut(s) 1303
BseMII CTCAG 2 cut(s) 510, 873
BseNI ACTGG 7 cut(s) 169, 179, 241, 592, 680, 741, 1318
BseXI GCAGC 3 cut(s) 9, 776, 1492
BsgI GTGCAG 1 cut(s) 1047
Bsh1236I CGCG 1 cut(s) 282
Bsh1285I CGRYCG 1 cut(s) 1699
BshFI GGCC 4 cut(s) 601, 980, 1082, 1153
BshTI ACCGGT 1 cut(s) 98
BsiEI CGRYCG 1 cut(s) 1699
BsiSI CCGG 2 cut(s) 99, 602
BslFI GGGAC 3 cut(s) 828, 1311, 1428
BslI CCNNNNNNNGG 2 cut(s) 767, 1698
BsmAI GTCTC 4 cut(s) 164, 578, 624, 1040
BsmFI GGGAC 3 cut(s) 828, 1311, 1428
BsmI GAATGC 4 cut(s) 40, 414, 1469, 1568
BsnI GGCC 4 cut(s) 601, 980, 1082, 1153
Bso31I GGTCTC 1 cut(s) 1040
Bsp119I TTCGAA 1 cut(s) 893
Bsp1286I GDGCHC 1 cut(s) 1208
Bsp143I GATC 9 cut(s) 109, 256, 1159, 1178, 1303, 1381, 1420, 1534, 1546
BspACI CCGC 2 cut(s) 476, 1695
BspANI GGCC 4 cut(s) 601, 980, 1082, 1153
BspCNI CTCAG 2 cut(s) 511, 872
BspFNI CGCG 1 cut(s) 282
BspHI TCATGA 2 cut(s) 418, 1561
BspLI GGNNCC 4 cut(s) 357, 600, 1180, 1327
BspMAI CTGCAG 4 cut(s) 27, 670, 766, 1608
BspMI ACCTGC 1 cut(s) 659
BspPI GGATC 4 cut(s) 264, 1173, 1186, 1529
BspT104I TTCGAA 1 cut(s) 893
BspTNI GGTCTC 1 cut(s) 1040
BsrDI GCAATG 1 cut(s) 1303
BsrFI RCCGGY 1 cut(s) 98
BsrI ACTGG 7 cut(s) 169, 179, 241, 592, 680, 741, 1318
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 2 cut(s) 810, 1257
BssMI GATC 9 cut(s) 109, 256, 1159, 1178, 1303, 1381, 1420, 1534, 1546
Bst2UI CCWGG 3 cut(s) 812, 1242, 1259
Bst4CI ACNGT 8 cut(s) 59, 335, 533, 975, 1057, 1369, 1664, 1672
Bst6I CTCTTC 4 cut(s) 1113, 1142, 1587, 1746
BstBI TTCGAA 1 cut(s) 893
BstC8I GCNNGC 5 cut(s) 12, 82, 222, 1608, 1612
BstDEI CTNAG 4 cut(s) 519, 747, 859, 1574
BstENI CCTNNNNNAGG 1 cut(s) 765
BstF5I GGATG 5 cut(s) 334, 395, 443, 484, 1027
BstFNI CGCG 1 cut(s) 282
BstHHI GCGC 3 cut(s) 40, 284, 612
BstKTI GATC 9 cut(s) 112, 259, 1162, 1181, 1306, 1384, 1423, 1537, 1549
BstMAI GTCTC 4 cut(s) 164, 578, 624, 1040
BstMBI GATC 9 cut(s) 109, 256, 1159, 1178, 1303, 1381, 1420, 1534, 1546
BstMCI CGRYCG 1 cut(s) 1699
BstMWI GCNNNNNNNGC 5 cut(s) 11, 493, 607, 1640, 1692
BstNI CCWGG 3 cut(s) 812, 1242, 1259
BstNSI RCATGY 2 cut(s) 1519, 1614
BstSCI CCNGG 4 cut(s) 601, 810, 1240, 1257
BstSFI CTRYAG 4 cut(s) 23, 666, 762, 1604
BstUI CGCG 1 cut(s) 282
BstV1I GCAGC 3 cut(s) 9, 776, 1492
BstX2I RGATCY 3 cut(s) 256, 1178, 1546
BstXI CCANNNNNNTGG 1 cut(s) 302
BstYI RGATCY 3 cut(s) 256, 1178, 1546
BsuRI GGCC 4 cut(s) 601, 980, 1082, 1153
BtsCI GGATG 5 cut(s) 334, 395, 443, 484, 1027
BtsI GCAGTG 1 cut(s) 1035
BtsIMutI CAGTG 5 cut(s) 162, 748, 1035, 1142, 1669
BveI ACCTGC 1 cut(s) 659
Cac8I GCNNGC 5 cut(s) 12, 82, 222, 1608, 1612
CciI TCATGA 2 cut(s) 418, 1561
CfoI GCGC 3 cut(s) 40, 284, 612
Cfr10I RCCGGY 1 cut(s) 98
Cfr13I GGNCC 3 cut(s) 599, 1152, 1325
Csp6I GTAC 6 cut(s) 278, 830, 1086, 1238, 1365, 1578
CspAI ACCGGT 1 cut(s) 98
CviQI GTAC 6 cut(s) 278, 830, 1086, 1238, 1365, 1578
DdeI CTNAG 4 cut(s) 519, 747, 859, 1574
DpnI GATC 9 cut(s) 111, 258, 1161, 1180, 1305, 1383, 1422, 1536, 1548
DpnII GATC 9 cut(s) 109, 256, 1159, 1178, 1303, 1381, 1420, 1534, 1546
DraI TTTAAA 1 cut(s) 732
DriI GACNNNNNGTC 1 cut(s) 92
EaeI YGGCCR 1 cut(s) 978
Eam1104I CTCTTC 4 cut(s) 1113, 1142, 1587, 1746
Eam1105I GACNNNNNGTC 1 cut(s) 92
EarI CTCTTC 4 cut(s) 1113, 1142, 1587, 1746
Eco147I AGGCCT 1 cut(s) 1082
Eco24I GRGCYC 1 cut(s) 1208
Eco31I GGTCTC 1 cut(s) 1040
Eco32I GATATC 1 cut(s) 366
Eco47I GGWCC 1 cut(s) 1325
Eco57I CTGAAG 5 cut(s) 1166, 1398, 1437, 1563, 1695
EcoNI CCTNNNNNAGG 1 cut(s) 765
EcoO109I RGGNCCY 1 cut(s) 1152
EcoRII CCWGG 3 cut(s) 810, 1240, 1257
EcoRV GATATC 1 cut(s) 366
EcoT22I ATGCAT 2 cut(s) 416, 1570
EcoT38I GRGCYC 1 cut(s) 1208
FaqI GGGAC 3 cut(s) 828, 1311, 1428
FauNDI CATATG 2 cut(s) 499, 1436
FbaI TGATCA 1 cut(s) 1159
FblI GTMKAC 1 cut(s) 1674
Fnu4HI GCNGC 3 cut(s) 23, 765, 1481
FokI GGATG 5 cut(s) 341, 402, 450, 491, 1034
FriOI GRGCYC 1 cut(s) 1208
Fsp4HI GCNGC 3 cut(s) 23, 765, 1481
FspBI CTAG 5 cut(s) 822, 900, 1401, 1431, 1644
GlaI GCGC 3 cut(s) 39, 283, 611
GluI GCNGC 3 cut(s) 23, 765, 1481
GsuI CTGGAG 3 cut(s) 246, 281, 1675
HaeIII GGCC 4 cut(s) 601, 980, 1082, 1153
HapII CCGG 2 cut(s) 99, 602
HhaI GCGC 3 cut(s) 40, 284, 612
Hin6I GCGC 3 cut(s) 38, 282, 610
HinP1I GCGC 3 cut(s) 38, 282, 610
HincII GTYRAC 1 cut(s) 958
HindII GTYRAC 1 cut(s) 958
HpaII CCGG 2 cut(s) 99, 602
HphI GGTGA 3 cut(s) 88, 116, 1465
Hpy166II GTNNAC 2 cut(s) 958, 1675
Hpy188I TCNGA 7 cut(s) 46, 920, 991, 1063, 1159, 1551, 1701
Hpy188III TCNNGA 9 cut(s) 260, 321, 396, 419, 623, 1108, 1163, 1562, 1754
Hpy8I GTNNAC 2 cut(s) 958, 1675
HpyAV CCTTC 3 cut(s) 334, 571, 1239
HpyCH4III ACNGT 8 cut(s) 59, 335, 533, 975, 1057, 1369, 1664, 1672
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 493, 607, 1640, 1692
HpyF3I CTNAG 4 cut(s) 519, 747, 859, 1574
HspAI GCGC 3 cut(s) 38, 282, 610
Ksp22I TGATCA 1 cut(s) 1159
Kzo9I GATC 9 cut(s) 109, 256, 1159, 1178, 1303, 1381, 1420, 1534, 1546
LmnI GCTCC 4 cut(s) 227, 355, 503, 780
Lsp1109I GCAGC 3 cut(s) 9, 776, 1492
LweI GCATC 6 cut(s) 428, 834, 1401, 1542, 1555, 1621
MaeI CTAG 5 cut(s) 822, 900, 1401, 1431, 1644
MaeIII GTNAC 3 cut(s) 94, 104, 682
MalI GATC 9 cut(s) 111, 258, 1161, 1180, 1305, 1383, 1422, 1536, 1548
MboI GATC 9 cut(s) 109, 256, 1159, 1178, 1303, 1381, 1420, 1534, 1546
MfeI CAATTG 1 cut(s) 132
MflI RGATCY 3 cut(s) 256, 1178, 1546
MhlI GDGCHC 1 cut(s) 1208
MluCI AATT 9 cut(s) 132, 199, 285, 454, 696, 700, 1016, 1124, 1500
MlyI GAGTC 5 cut(s) 166, 474, 851, 1243, 1711
MmeI TCCRAC 1 cut(s) 1679
Mph1103I ATGCAT 2 cut(s) 416, 1570
MroXI GAANNNNTTC 1 cut(s) 700
MseI TTAA 7 cut(s) 54, 152, 198, 492, 699, 731, 1212
MslI CAYNNNNRTG 3 cut(s) 300, 1199, 1615
MspA1I CMGCKG 1 cut(s) 1695
MspI CCGG 2 cut(s) 99, 602
MspR9I CCNGG 4 cut(s) 603, 812, 1242, 1259
MunI CAATTG 1 cut(s) 132
Mva1269I GAATGC 4 cut(s) 40, 414, 1469, 1568
MvaI CCWGG 3 cut(s) 812, 1242, 1259
MvnI CGCG 1 cut(s) 282
MwoI GCNNNNNNNGC 5 cut(s) 11, 493, 607, 1640, 1692
NciI CCSGG 1 cut(s) 603
NdeI CATATG 2 cut(s) 499, 1436
NdeII GATC 9 cut(s) 109, 256, 1159, 1178, 1303, 1381, 1420, 1534, 1546
NlaIV GGNNCC 4 cut(s) 357, 600, 1180, 1327
NmuCI GTSAC 2 cut(s) 94, 104
NsiI ATGCAT 2 cut(s) 416, 1570
NspI RCATGY 2 cut(s) 1519, 1614
NspV TTCGAA 1 cut(s) 893
PaeI GCATGC 1 cut(s) 1614
PagI TCATGA 2 cut(s) 418, 1561
PaqCI CACCTGC 1 cut(s) 659
PceI AGGCCT 1 cut(s) 1082
PciI ACATGT 1 cut(s) 1515
PctI GAATGC 4 cut(s) 40, 414, 1469, 1568
PdmI GAANNNNTTC 1 cut(s) 700
PfeI GAWTC 7 cut(s) 185, 374, 567, 662, 757, 878, 986
PinAI ACCGGT 1 cut(s) 98
PkrI GCNGC 3 cut(s) 24, 766, 1482
PleI GAGTC 5 cut(s) 165, 473, 851, 1243, 1710
PpsI GAGTC 5 cut(s) 165, 473, 851, 1243, 1710
PscI ACATGT 1 cut(s) 1515
PshBI ATTAAT 1 cut(s) 699
Psp6I CCWGG 3 cut(s) 810, 1240, 1257
PspGI CCWGG 3 cut(s) 810, 1240, 1257
PspN4I GGNNCC 4 cut(s) 357, 600, 1180, 1327
PspPI GGNCC 3 cut(s) 599, 1152, 1325
PstI CTGCAG 4 cut(s) 27, 670, 766, 1608
PsuI RGATCY 3 cut(s) 256, 1178, 1546
RsaI GTAC 6 cut(s) 279, 831, 1087, 1239, 1366, 1579
RsaNI GTAC 6 cut(s) 278, 830, 1086, 1238, 1365, 1578
RseI CAYNNNNRTG 3 cut(s) 300, 1199, 1615
SaqAI TTAA 7 cut(s) 54, 152, 198, 492, 699, 731, 1212
SatI GCNGC 3 cut(s) 23, 765, 1481
Sau3AI GATC 9 cut(s) 109, 256, 1159, 1178, 1303, 1381, 1420, 1534, 1546
Sau96I GGNCC 3 cut(s) 599, 1152, 1325
ScaI AGTACT 1 cut(s) 1579
SchI GAGTC 5 cut(s) 166, 474, 851, 1243, 1711
ScrFI CCNGG 4 cut(s) 603, 812, 1242, 1259
SduI GDGCHC 1 cut(s) 1208
SfaNI GCATC 6 cut(s) 428, 834, 1401, 1542, 1555, 1621
SfcI CTRYAG 4 cut(s) 23, 666, 762, 1604
SfuI TTCGAA 1 cut(s) 893
SgrAI CRCCGGYG 1 cut(s) 98
SinI GGWCC 1 cut(s) 1325
SmiMI CAYNNNNRTG 3 cut(s) 300, 1199, 1615
SphI GCATGC 1 cut(s) 1614
Sse9I AATT 9 cut(s) 132, 199, 285, 454, 696, 700, 1016, 1124, 1500
SseBI AGGCCT 1 cut(s) 1082
SsiI CCGC 2 cut(s) 476, 1695
SspI AATATT 3 cut(s) 52, 67, 315
SspMI CTAG 5 cut(s) 822, 900, 1401, 1431, 1644
StuI AGGCCT 1 cut(s) 1082
StyD4I CCNGG 4 cut(s) 601, 810, 1240, 1257
TaaI ACNGT 8 cut(s) 59, 335, 533, 975, 1057, 1369, 1664, 1672
TaqI TCGA 3 cut(s) 451, 835, 893
TasI AATT 9 cut(s) 132, 199, 285, 454, 696, 700, 1016, 1124, 1500
TatI WGTACW 1 cut(s) 1577
TfiI GAWTC 7 cut(s) 185, 374, 567, 662, 757, 878, 986
Tru1I TTAA 7 cut(s) 54, 152, 198, 492, 699, 731, 1212
Tru9I TTAA 7 cut(s) 54, 152, 198, 492, 699, 731, 1212
TscAI CASTG 5 cut(s) 169, 748, 1035, 1149, 1669
TseFI GTSAC 2 cut(s) 94, 104
TseI GCWGC 3 cut(s) 22, 764, 1480
Tsp45I GTSAC 2 cut(s) 94, 104
TspDTI ATGAA 4 cut(s) 339, 407, 804, 1470
TspGWI ACGGA 1 cut(s) 1103
TspRI CASTG 5 cut(s) 169, 748, 1035, 1149, 1669
VpaK11BI GGWCC 1 cut(s) 1325
VspI ATTAAT 1 cut(s) 699
XagI CCTNNNNNAGG 1 cut(s) 765
XapI RAATTY 3 cut(s) 454, 1124, 1500
XceI RCATGY 2 cut(s) 1519, 1614
XmiI GTMKAC 1 cut(s) 1674
XmnI GAANNNNTTC 1 cut(s) 700
XspI CTAG 5 cut(s) 822, 900, 1401, 1431, 1644
ZrmI AGTACT 1 cut(s) 1579
Zsp2I ATGCAT 2 cut(s) 416, 1570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.