Rroxscaffold_1G00069050

May participate in a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001097
Physical Location & Seq
Forward (+)
90067064 .. 90080702
13639 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00069050.1

Sequence Viewer

Length: 2583 bp
ATGGCAAGTAGGCAAAGATGGGTGCCATGCATGATAGCTAGTTTGGAGTTTGTGGCGCATTCTGACAATATTAACTGTCTAAATATTGGGAAAAAGGCGTGCCGACTTTATGTCACCGGTGGTGATGATCACAAAGTCAATCTATGGACAATTGGCAAACCTACTGCGTTAATGAGTCTCACTGGTCATACAAGTCCAGTTGAATCTGTAGCTTTTAATTCAGCAGAAGTTTTGGTGCTTGCTGGAGCTTCTACTGGTGCAACAAAAATATGGGATCTGGAGGAAACAAAGATGGTTCGCACACTTACCGGACACAGATCCAGCTGTTCTGCTGTTGAATTTCATCCATTTGGTGAGTTCTTTGCTTCTGGTTCCATGGACACTAATCTGAAGATCTGGGACATTAGAAAGAAGGGATGCATCCACACATACAAAGGTCATACTCAAGGCATTAGTACTATTAAGTTCACTCCCGATGGTCGTTGGGTAGTTTCTGGTGGGTTTGACAATGTTGTAAAGGTGTGGGATCTAACTGCTGGGAAGCTCTTGCATGATTTTAAATTCCATGAAGGACATATTCGGTCTATAGATTTTCATCCCCTTGAGTTTCTCTTGGCCACAGCAACCAGTAAATTGCTTGAATGTACAAAACATGTTCCAGGTTCAGCAGACAGAACTGTGAAATTCTGGGATCTGGAAACTTTTGAACTGATTGGATCTACCAGGCCTGAGCCTACAGGAGTACGTGCAATTGCTTTCCACCCTGATGGGAGGACAATATTTTCTGGATTGGATGACAGTTTGAAGGTTTATTCATGGGAGCCTGTAATAGCCCATGATTCTGTTGATATGGGTTGGTCAAGACTTGCTGACTTATGCGTTCATGACGGGAAGCTATTGGGATGCTCTTACTATCGAAATTCTATTGGAGTCTGGGTTGTGGATGTGTCGCTTATTAAGCCATATGGAGCTGGTTCAACACCTGAGCAAAATGACTGTTTAGATGAGAAATCTCATGTAAAGTTGCAAGGAATCATGGAGAAGGTAGAGACTGGTATAAGGGGTCCGGGTTTCCGCTGTACGTCTCCTGATTATGACACAAAGGAAATAAAGAACATATATGTTGATTCTGCAGGTGGAAAGCCAGTTACCTCACAGAGAATTAATTCTTCAAAAGTTTTACCCCCATTGGATTTAAAGGAAACCAGTGCTAAGAAGCAGATTCCTGCAGCAGGGTCGCTCCCAAAGTCTAATGAAAAGACGGTCAATAAATCCCTGGTTGTGCCTAGCATCGTACCTCGAAGCAGTCCCAATGCAAAGGATTCAGCTAACTATGGAAGAGAATCTGTTGCATTTTCGAAAACTAGGCGTGGTATGTTGCTCAGACCAGCTCATGTCCGAAGCCCATCAAATAGCAAACTTGACGTGGAGAAGCTACCGTTGGCCGTTGAATCTGAAACTTTCAGCAGTATGACTAGTAAATTGGATGGTGCAGTGGGTCTCAGTTCTCAAATCAAAACTGTATCAGAAGATAGTGATAGAGAGGCCTGTAAGGAAAAACATTCTGCAATCAAGAGTGTAGAAGAGAAATTTGAAGCAGTATTATCACTGGAGAGGCTCTCTGATCAGGAAACCAGAAATGGATCCCTCAATAACAGCAAGGAAGTGAGCCCCGTTAAAGTTATCAATGGAGTTGCCGTTGTACCGGGAAGGACTCGCACCCTGGTTGAGAGATTTGAAAGGAGAGAGAGATTTAGTAGCAATGACGATCAAGCAACTGGCGTATTTGGTCCCGTGATACCTGAATATCAAGCAACCAGCATATCTCGTACTGTGATACCTGAAGATCAAGCAAGTAGCATATCTAGTCCTGTGATGCCTGAAGATCAAGCAACTAGCATATGTAGTCCCGTGATACATGAAATGGATAAAACATTCACCATGCAGCGAGAAGAAGAACCTCAAATTTCTGGGAGGCACATGTCATCTGCAAACAATGATCCTGCTGAAGATCTGATGCAAACTCATGATGCATTCCTAAGTACCCTTCGATCCCGCCTGACAAAACTGCAGGTGGTGAGGCATTTTTGGGAACGAAATGATATTAAAGGTGCTATCAGTGCTATCAGAAAGCTGCCAGATCATTCGGCATGCAAATGTGTCATTGGAGATGCTGTTGAAGCTAGTAGCAGTCTTTGGCCCAGTGATACGGTCTACCATTTCAGCACCTCCAGCGGTCGGAGTCAATCTTCAGGCAGAGCAAAGGCTGGAGTGCTGCAACCAATGCTCTGTACACCTGCAAAAGATCCAGAAAATTCTCCCAGTCTTTGTAAGACTAGAGCTCAGGAATTTTGTGAGGCAAAGATCAAAGTAATATCAGTCTTGCCTGAGGAGCTTGAAGACAGTAGTCATCCCTTCCTAGAGTCATCTTCGGCATTACTTAATATTGGTCTACTAGAGCTCAAGAATTTTGTGAGGACAGTCTTGCCTGAGGAGCTTCAAGACAGGAGAAGTGATCCCTGCAGTATTCGCGCCGTCTTTGTTCGCAAACACTCTGATGAGGAACCAAAGAGCAGATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Katanin_con80 WD40

Protein Analysis

860

Amino Acids

94.7

Weight (kDa)

6.96

Isoelectric Point (pI)

47.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_SCAP PF24017 10 - 111 7.9e-06 SCAP Beta-propeller
WD40_Gbeta PF25391 14 - 137 4.8e-14 G protein beta WD-40 repeat protein
Beta-prop_WDR3_1st PF25173 15 - 101 1.4e-10 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 17 - 143 5.4e-25 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 19 - 138 4.2e-11 WDR36/Utp21 second beta-propeller domain
Beta-prop_EML PF23409 20 - 213 2.3e-10 Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19 PF24814 21 - 210 5.4e-37 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 21 - 179 1.5e-19 WDR3 second beta-propeller domain
WD40_WDHD1_1st PF24817 29 - 146 7.2e-17 WDHD1 first WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 33 - 133 2.2e-07 WDR36/Utp21 first beta-propeller
Beta-prop_EML_2 PF23414 35 - 162 8.4e-16 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 38 - 138 9.5e-06 WDR90/POC16, second beta-propeller
WD40_CDC20-Fz PF24807 55 - 264 4.9e-29 CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 57 - 143 2.2e-06 CAF1B/HIR1 beta-propeller domain
EIF3I PF24805 58 - 144 5.2e-07 EIF3I
Beta-prop_SPT8 PF23798 63 - 158 1.7e-06 SPT8 beta propeller
Beta-prop_THOC3 PF25174 65 - 208 6.4e-32 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 67 - 210 1.5e-30 WDR3 first beta-propeller domain
WDR55 PF24796 68 - 209 2.7e-13 WDR55
WD40_MABP1-WDR62_2nd PF24782 84 - 177 9.8e-09 MABP1/WDR62 second WD40 domain
Beta-prop_EML_2 PF23414 90 - 201 1.7e-13 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 97 - 134 2e-09 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 98 - 196 1.9e-08 TEP-1 second beta-propeller
EIF3I PF24805 102 - 213 3.3e-11 EIF3I
Beta-prop_WDR5 PF25175 104 - 208 2e-24 WDR5 beta-propeller domain
Beta-prop_Aladin PF25460 107 - 207 2.6e-08 Aladin seven-bladed propeller
Beta-prop_WDR36-Utp21_1st PF25171 120 - 175 5.1e-06 WDR36/Utp21 first beta-propeller
WD40_Gbeta PF25391 126 - 266 5e-10 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 134 - 210 5.3e-12 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 140 - 176 8.5e-12 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 142 - 210 6.2e-07 CAF1B/HIR1 beta-propeller domain
EIF3I PF24805 145 - 269 1.5e-09 EIF3I
Katanin_con80 PF13925 674 - 717 3.9e-12 con80 domain of Katanin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 1124, 2062, 2305
Acc36I ACCTGC 3 cut(s) 1124, 2062, 2305
AccB1I GGYRCC 1 cut(s) 22
AccI GTMKAC 2 cut(s) 2214, 2452
AccII CGCG 1 cut(s) 2532
AciI CCGC 3 cut(s) 1075, 2056, 2235
AcoI YGGCCR 2 cut(s) 615, 1443
AcsI RAATTY 9 cut(s) 338, 560, 683, 919, 1589, 1965, 2314, 2348, 2467
AcuI CTGAAG 5 cut(s) 410, 1863, 1902, 2028, 2235
AfaI GTAC 9 cut(s) 457, 646, 744, 1081, 1296, 1704, 1831, 2044, 2293
AfiI CCNNNNNNNGG 2 cut(s) 1232, 2238
AflIII ACRYGT 2 cut(s) 652, 1980
AgeI ACCGGT 1 cut(s) 116
AhdI GACNNNNNGTC 1 cut(s) 110
AhlI ACTAGT 1 cut(s) 1475
AjiI CACGTC 1 cut(s) 1426
AjnI CCWGG 4 cut(s) 658, 722, 1275, 1722
AjuI GAANNNNNNNTTGG 2 cut(s) 1424, 1456
Alw21I GWGCWC 2 cut(s) 2344, 2463
Alw26I GTCTC 4 cut(s) 182, 1043, 1089, 1505
AoxI GGCC 5 cut(s) 615, 725, 1443, 1545, 2198
ApeKI GCWGC 4 cut(s) 1229, 1945, 2134, 2275
ApoI RAATTY 9 cut(s) 338, 560, 683, 919, 1589, 1965, 2314, 2348, 2467
AseI ATTAAT 1 cut(s) 1164
AsiGI ACCGGT 1 cut(s) 116
Asp700I GAANNNNTTC 1 cut(s) 1165
AspLEI GCGC 2 cut(s) 58, 2534
AspS9I GGNCC 3 cut(s) 1064, 1790, 2199
AsuC2I CCSGG 2 cut(s) 1068, 1707
AsuHPI GGTGA 5 cut(s) 106, 134, 365, 1930, 2089
AsuII TTCGAA 1 cut(s) 1358
AvaII GGWCC 2 cut(s) 1064, 1790
AxyI CCTNAGG 2 cut(s) 2388, 2490
BalI TGGCCA 1 cut(s) 617
BamHI GGATCC 1 cut(s) 1643
BanI GGYRCC 1 cut(s) 22
BanII GRGCYC 3 cut(s) 1673, 2344, 2463
BbsI GAAGAC 1 cut(s) 2406
Bbv12I GWGCWC 2 cut(s) 2344, 2463
BbvI GCAGC 4 cut(s) 1241, 1957, 2121, 2262
BccI CCATC 6 cut(s) 12, 286, 470, 761, 1414, 1481
BceAI ACGGC 3 cut(s) 1430, 1682, 2519
BcgI CGANNNNNNTGC 2 cut(s) 1218, 1252
BciT130I CCWGG 4 cut(s) 660, 724, 1277, 1724
BclI TGATCA 2 cut(s) 127, 1624
BcnI CCSGG 2 cut(s) 1068, 1707
BcoDI GTCTC 4 cut(s) 182, 1043, 1089, 1505
BcuI ACTAGT 1 cut(s) 1475
BfmI CTRYAG 7 cut(s) 207, 585, 735, 1131, 1227, 2069, 2521
BfuAI ACCTGC 3 cut(s) 1124, 2062, 2305
BglII AGATCT 2 cut(s) 393, 2011
BisI GCNGC 4 cut(s) 1230, 1946, 2135, 2276
BlsI GCNGC 4 cut(s) 1231, 1947, 2136, 2277
BmcAI AGTACT 1 cut(s) 457
Bme1390I CCNGG 6 cut(s) 660, 724, 1068, 1277, 1707, 1724
Bme18I GGWCC 2 cut(s) 1064, 1790
BmeRI GACNNNNNGTC 1 cut(s) 110
BmgBI CACGTC 1 cut(s) 1426
BmgT120I GGNCC 3 cut(s) 1064, 1790, 2199
BmiI GGNNCC 7 cut(s) 24, 373, 822, 1065, 1645, 1792, 2565
BmrFI CCNGG 6 cut(s) 660, 724, 1068, 1277, 1707, 1724
BmrI ACTGGG 2 cut(s) 2196, 2316
BmsI GCATC 8 cut(s) 407, 429, 893, 1299, 1866, 2007, 2020, 2161
BmuI ACTGGG 2 cut(s) 2196, 2316
BoxI GACNNNNGTC 1 cut(s) 2406
BpiI GAAGAC 1 cut(s) 2406
BplI GAGNNNNNCTC 2 cut(s) 1604, 1636
BpmI CTGGAG 5 cut(s) 264, 299, 1631, 2215, 2289
Bpu10I CCTNAGC 3 cut(s) 729, 984, 2343
Bpu14I TTCGAA 1 cut(s) 1358
BpuEI CTTGAG 3 cut(s) 429, 623, 2447
BpuMI CCSGG 2 cut(s) 1068, 1707
BsaAI YACGTR 1 cut(s) 746
BsaBI GATNNNNATC 1 cut(s) 594
BsaI GGTCTC 1 cut(s) 1505
BsaJI CCNNGG 3 cut(s) 375, 1275, 1722
BsaWI WCCGGW 2 cut(s) 116, 308
BsaXI ACNNNNNCTCC 6 cut(s) 921, 951, 1683, 1713, 2160, 2190
Bsc4I CCNNNNNNNGG 2 cut(s) 1232, 2238
Bse118I RCCGGY 1 cut(s) 116
Bse21I CCTNAGG 2 cut(s) 2388, 2490
Bse3DI GCAATG 1 cut(s) 1768
Bse8I GATNNNNATC 1 cut(s) 594
BseBI CCWGG 4 cut(s) 660, 724, 1277, 1724
BseDI CCNNGG 3 cut(s) 375, 1275, 1722
BseGI GGATG 9 cut(s) 343, 420, 422, 595, 799, 908, 949, 1492, 2410
BseJI GATNNNNATC 1 cut(s) 594
BseLI CCNNNNNNNGG 2 cut(s) 1232, 2238
BseMI GCAATG 1 cut(s) 1768
BseMII CTCAG 7 cut(s) 720, 975, 1396, 1516, 2357, 2379, 2481
BseRI GAGGAG 2 cut(s) 2405, 2507
BseXI GCAGC 4 cut(s) 1241, 1957, 2121, 2262
BseYI CCCAGC 1 cut(s) 536
BsgI GTGCAG 1 cut(s) 1512
Bsh1236I CGCG 1 cut(s) 2532
Bsh1285I CGRYCG 1 cut(s) 2239
BshFI GGCC 5 cut(s) 617, 727, 1445, 1547, 2200
BshNI GGYRCC 1 cut(s) 22
BshTI ACCGGT 1 cut(s) 116
BsiEI CGRYCG 1 cut(s) 2239
BsiHKAI GWGCWC 2 cut(s) 2344, 2463
BsiSI CCGG 4 cut(s) 117, 309, 1067, 1706
BslFI GGGAC 4 cut(s) 413, 1293, 1776, 1893
BslI CCNNNNNNNGG 2 cut(s) 1232, 2238
BsmAI GTCTC 4 cut(s) 182, 1043, 1089, 1505
BsmBI CGTCTC 1 cut(s) 1089
BsmFI GGGAC 4 cut(s) 413, 1293, 1776, 1893
BsmI GAATGC 2 cut(s) 58, 2033
BsnI GGCC 5 cut(s) 617, 727, 1445, 1547, 2200
Bso31I GGTCTC 1 cut(s) 1505
Bsp119I TTCGAA 1 cut(s) 1358
Bsp1286I GDGCHC 3 cut(s) 1673, 2344, 2463
Bsp1407I TGTACA 2 cut(s) 644, 2291
Bsp19I CCATGG 1 cut(s) 375
BspACI CCGC 3 cut(s) 1075, 2056, 2235
BspANI GGCC 5 cut(s) 617, 727, 1445, 1547, 2200
BspCNI CTCAG 7 cut(s) 721, 976, 1395, 1515, 2356, 2380, 2482
BspFNI CGCG 1 cut(s) 2532
BspHI TCATGA 2 cut(s) 883, 2026
BspLI GGNNCC 7 cut(s) 24, 373, 822, 1065, 1645, 1792, 2565
BspMAI CTGCAG 4 cut(s) 1135, 1231, 2073, 2525
BspMI ACCTGC 3 cut(s) 1124, 2062, 2305
BspT104I TTCGAA 1 cut(s) 1358
BspT107I GGYRCC 1 cut(s) 22
BspTNI GGTCTC 1 cut(s) 1505
BsrDI GCAATG 1 cut(s) 1768
BsrFI RCCGGY 1 cut(s) 116
BsrGI TGTACA 2 cut(s) 644, 2291
BssAI RCCGGY 1 cut(s) 116
BssECI CCNNGG 3 cut(s) 375, 1275, 1722
BssT1I CCWWGG 1 cut(s) 375
Bst2UI CCWGG 4 cut(s) 660, 724, 1277, 1724
Bst6I CTCTTC 2 cut(s) 1333, 1578
BstAPI GCANNNNNTGC 1 cut(s) 2284
BstAUI TGTACA 2 cut(s) 644, 2291
BstBAI YACGTR 1 cut(s) 746
BstBI TTCGAA 1 cut(s) 1358
BstC8I GCNNGC 3 cut(s) 100, 240, 2152
BstDEI CTNAG 9 cut(s) 729, 984, 1212, 1382, 1502, 2039, 2343, 2388, 2490
BstDSI CCRYGG 1 cut(s) 375
BstENI CCTNNNNNAGG 1 cut(s) 1230
BstF5I GGATG 9 cut(s) 343, 420, 422, 595, 799, 908, 949, 1492, 2410
BstFNI CGCG 1 cut(s) 2532
BstHHI GCGC 2 cut(s) 58, 2534
BstMAI GTCTC 4 cut(s) 182, 1043, 1089, 1505
BstMCI CGRYCG 1 cut(s) 2239
BstMWI GCNNNNNNNGC 8 cut(s) 958, 2120, 2180, 2232, 2284, 2392, 2494, 2529
BstNI CCWGG 4 cut(s) 660, 724, 1277, 1724
BstNSI RCATGY 3 cut(s) 656, 1984, 2154
BstPAI GACNNNNGTC 1 cut(s) 2406
BstSCI CCNGG 6 cut(s) 658, 722, 1066, 1275, 1705, 1722
BstSFI CTRYAG 7 cut(s) 207, 585, 735, 1131, 1227, 2069, 2521
BstUI CGCG 1 cut(s) 2532
BstV1I GCAGC 4 cut(s) 1241, 1957, 2121, 2262
BstV2I GAAGAC 1 cut(s) 2406
BstX2I RGATCY 9 cut(s) 274, 317, 393, 526, 691, 716, 1643, 2011, 2305
BstXI CCANNNNNNTGG 1 cut(s) 767
BstYI RGATCY 9 cut(s) 274, 317, 393, 526, 691, 716, 1643, 2011, 2305
Bsu36I CCTNAGG 2 cut(s) 2388, 2490
BsuRI GGCC 5 cut(s) 617, 727, 1445, 1547, 2200
BtgI CCRYGG 1 cut(s) 375
BtrI CACGTC 1 cut(s) 1426
BtsCI GGATG 9 cut(s) 343, 420, 422, 595, 799, 908, 949, 1492, 2410
BtsI GCAGTG 1 cut(s) 1500
BtsIMutI CAGTG 6 cut(s) 180, 1213, 1500, 1607, 2125, 2209
BveI ACCTGC 3 cut(s) 1124, 2062, 2305
Cac8I GCNNGC 3 cut(s) 100, 240, 2152
CciI TCATGA 2 cut(s) 883, 2026
CfoI GCGC 2 cut(s) 58, 2534
Cfr10I RCCGGY 1 cut(s) 116
Cfr13I GGNCC 3 cut(s) 1064, 1790, 2199
Csp6I GTAC 9 cut(s) 456, 645, 743, 1080, 1295, 1703, 1830, 2043, 2292
CspAI ACCGGT 1 cut(s) 116
CviQI GTAC 9 cut(s) 456, 645, 743, 1080, 1295, 1703, 1830, 2043, 2292
DdeI CTNAG 9 cut(s) 729, 984, 1212, 1382, 1502, 2039, 2343, 2388, 2490
DraI TTTAAA 2 cut(s) 559, 1197
DriI GACNNNNNGTC 1 cut(s) 110
EaeI YGGCCR 2 cut(s) 615, 1443
Eam1104I CTCTTC 2 cut(s) 1333, 1578
Eam1105I GACNNNNNGTC 1 cut(s) 110
EarI CTCTTC 2 cut(s) 1333, 1578
Ecl136II GAGCTC 2 cut(s) 2342, 2461
Eco130I CCWWGG 1 cut(s) 375
Eco147I AGGCCT 2 cut(s) 727, 1547
Eco24I GRGCYC 3 cut(s) 1673, 2344, 2463
Eco31I GGTCTC 1 cut(s) 1505
Eco47I GGWCC 2 cut(s) 1064, 1790
Eco53kI GAGCTC 2 cut(s) 2342, 2461
Eco57I CTGAAG 5 cut(s) 410, 1863, 1902, 2028, 2235
Eco81I CCTNAGG 2 cut(s) 2388, 2490
EcoICRI GAGCTC 2 cut(s) 2342, 2461
EcoNI CCTNNNNNAGG 1 cut(s) 1230
EcoRII CCWGG 4 cut(s) 658, 722, 1275, 1722
EcoT14I CCWWGG 1 cut(s) 375
EcoT22I ATGCAT 3 cut(s) 32, 422, 2035
EcoT38I GRGCYC 3 cut(s) 1673, 2344, 2463
ErhI CCWWGG 1 cut(s) 375
Esp3I CGTCTC 1 cut(s) 1089
FaqI GGGAC 4 cut(s) 413, 1293, 1776, 1893
FauI CCCGC 1 cut(s) 2063
FauNDI CATATG 2 cut(s) 964, 1901
FbaI TGATCA 2 cut(s) 127, 1624
FblI GTMKAC 2 cut(s) 2214, 2452
Fnu4HI GCNGC 4 cut(s) 1230, 1946, 2135, 2276
FokI GGATG 9 cut(s) 330, 407, 429, 582, 806, 915, 956, 1499, 2397
FriOI GRGCYC 3 cut(s) 1673, 2344, 2463
Fsp4HI GCNGC 4 cut(s) 1230, 1946, 2135, 2276
GlaI GCGC 2 cut(s) 57, 2533
GluI GCNGC 4 cut(s) 1230, 1946, 2135, 2276
GsaI CCCAGC 1 cut(s) 540
GsuI CTGGAG 5 cut(s) 264, 299, 1631, 2215, 2289
HaeIII GGCC 5 cut(s) 617, 727, 1445, 1547, 2200
HapII CCGG 4 cut(s) 117, 309, 1067, 1706
HhaI GCGC 2 cut(s) 58, 2534
Hin6I GCGC 2 cut(s) 56, 2532
HinP1I GCGC 2 cut(s) 56, 2532
HpaII CCGG 4 cut(s) 117, 309, 1067, 1706
HphI GGTGA 5 cut(s) 106, 134, 365, 1930, 2089
Hpy166II GTNNAC 4 cut(s) 468, 2215, 2294, 2453
Hpy8I GTNNAC 4 cut(s) 468, 2215, 2294, 2453
HpyAV CCTTC 7 cut(s) 406, 563, 799, 1036, 1704, 2057, 2425
HpyCH4IV ACGT 3 cut(s) 745, 1082, 1425
HpyF10VI GCNNNNNNNGC 8 cut(s) 958, 2120, 2180, 2232, 2284, 2392, 2494, 2529
HpyF3I CTNAG 9 cut(s) 729, 984, 1212, 1382, 1502, 2039, 2343, 2388, 2490
HpySE526I ACGT 3 cut(s) 745, 1082, 1425
HspAI GCGC 2 cut(s) 56, 2532
Ksp22I TGATCA 2 cut(s) 127, 1624
LmnI GCTCC 6 cut(s) 245, 820, 968, 1245, 2392, 2494
Lsp1109I GCAGC 4 cut(s) 1241, 1957, 2121, 2262
LweI GCATC 8 cut(s) 407, 429, 893, 1299, 1866, 2007, 2020, 2161
MaeII ACGT 3 cut(s) 745, 1082, 1425
MaeIII GTNAC 2 cut(s) 112, 1147
MfeI CAATTG 2 cut(s) 150, 750
MflI RGATCY 9 cut(s) 274, 317, 393, 526, 691, 716, 1643, 2011, 2305
MhlI GDGCHC 3 cut(s) 1673, 2344, 2463
MlsI TGGCCA 1 cut(s) 617
MluNI TGGCCA 1 cut(s) 617
MlyI GAGTC 5 cut(s) 184, 939, 1708, 2251, 2432
MmeI TCCRAC 1 cut(s) 2219
Mox20I TGGCCA 1 cut(s) 617
Mph1103I ATGCAT 3 cut(s) 32, 422, 2035
MroXI GAANNNNTTC 1 cut(s) 1165
MscI TGGCCA 1 cut(s) 617
MslI CAYNNNNRTG 3 cut(s) 765, 2155, 2556
Msp20I TGGCCA 1 cut(s) 617
MspA1I CMGCKG 3 cut(s) 324, 1077, 2235
MspI CCGG 4 cut(s) 117, 309, 1067, 1706
MspR9I CCNGG 6 cut(s) 660, 724, 1068, 1277, 1707, 1724
MunI CAATTG 2 cut(s) 150, 750
Mva1269I GAATGC 2 cut(s) 58, 2033
MvaI CCWGG 4 cut(s) 660, 724, 1277, 1724
MvnI CGCG 1 cut(s) 2532
MwoI GCNNNNNNNGC 8 cut(s) 958, 2120, 2180, 2232, 2284, 2392, 2494, 2529
NciI CCSGG 2 cut(s) 1068, 1707
NcoI CCATGG 1 cut(s) 375
NdeI CATATG 2 cut(s) 964, 1901
NlaIV GGNNCC 7 cut(s) 24, 373, 822, 1065, 1645, 1792, 2565
NmuCI GTSAC 1 cut(s) 112
NsiI ATGCAT 3 cut(s) 32, 422, 2035
NspI RCATGY 3 cut(s) 656, 1984, 2154
NspV TTCGAA 1 cut(s) 1358
PaeI GCATGC 1 cut(s) 2154
PagI TCATGA 2 cut(s) 883, 2026
PaqCI CACCTGC 3 cut(s) 1124, 2062, 2305
PceI AGGCCT 2 cut(s) 727, 1547
PciI ACATGT 2 cut(s) 652, 1980
PctI GAATGC 2 cut(s) 58, 2033
PdmI GAANNNNTTC 1 cut(s) 1165
PfeI GAWTC 8 cut(s) 203, 839, 1032, 1127, 1222, 1322, 1343, 1451
PinAI ACCGGT 1 cut(s) 116
PkrI GCNGC 4 cut(s) 1231, 1947, 2136, 2277
PleI GAGTC 5 cut(s) 183, 938, 1708, 2250, 2431
PpsI GAGTC 5 cut(s) 183, 938, 1708, 2250, 2431
Ppu21I YACGTR 1 cut(s) 746
PscI ACATGT 2 cut(s) 652, 1980
PshAI GACNNNNGTC 1 cut(s) 2406
PshBI ATTAAT 1 cut(s) 1164
Psp124BI GAGCTC 2 cut(s) 2344, 2463
Psp6I CCWGG 4 cut(s) 658, 722, 1275, 1722
PspFI CCCAGC 1 cut(s) 536
PspGI CCWGG 4 cut(s) 658, 722, 1275, 1722
PspN4I GGNNCC 7 cut(s) 24, 373, 822, 1065, 1645, 1792, 2565
PspPI GGNCC 3 cut(s) 1064, 1790, 2199
PstI CTGCAG 4 cut(s) 1135, 1231, 2073, 2525
PsuI RGATCY 9 cut(s) 274, 317, 393, 526, 691, 716, 1643, 2011, 2305
PvuII CAGCTG 1 cut(s) 324
RsaI GTAC 9 cut(s) 457, 646, 744, 1081, 1296, 1704, 1831, 2044, 2293
RsaNI GTAC 9 cut(s) 456, 645, 743, 1080, 1295, 1703, 1830, 2043, 2292
RseI CAYNNNNRTG 3 cut(s) 765, 2155, 2556
SacI GAGCTC 2 cut(s) 2344, 2463
SatI GCNGC 4 cut(s) 1230, 1946, 2135, 2276
Sau96I GGNCC 3 cut(s) 1064, 1790, 2199
ScaI AGTACT 1 cut(s) 457
SchI GAGTC 5 cut(s) 184, 939, 1708, 2251, 2432
ScrFI CCNGG 6 cut(s) 660, 724, 1068, 1277, 1707, 1724
SduI GDGCHC 3 cut(s) 1673, 2344, 2463
SfaNI GCATC 8 cut(s) 407, 429, 893, 1299, 1866, 2007, 2020, 2161
SfcI CTRYAG 7 cut(s) 207, 585, 735, 1131, 1227, 2069, 2521
SfuI TTCGAA 1 cut(s) 1358
SgrAI CRCCGGYG 1 cut(s) 116
SinI GGWCC 2 cut(s) 1064, 1790
SmiMI CAYNNNNRTG 3 cut(s) 765, 2155, 2556
SmlI CTYRAG 3 cut(s) 444, 602, 2462
SmoI CTYRAG 3 cut(s) 444, 602, 2462
SpeI ACTAGT 1 cut(s) 1475
SphI GCATGC 1 cut(s) 2154
SseBI AGGCCT 2 cut(s) 727, 1547
SsiI CCGC 3 cut(s) 1075, 2056, 2235
SspI AATATT 4 cut(s) 70, 85, 780, 2446
SstI GAGCTC 2 cut(s) 2344, 2463
StuI AGGCCT 2 cut(s) 727, 1547
StyD4I CCNGG 6 cut(s) 658, 722, 1066, 1275, 1705, 1722
StyI CCWWGG 1 cut(s) 375
TaiI ACGT 3 cut(s) 748, 1085, 1428
TaqI TCGA 4 cut(s) 916, 1300, 1358, 2050
TaqII GACCGA 1 cut(s) 570
TatI WGTACW 3 cut(s) 455, 644, 2291
TfiI GAWTC 8 cut(s) 203, 839, 1032, 1127, 1222, 1322, 1343, 1451
TscAI CASTG 6 cut(s) 187, 1213, 1500, 1614, 2125, 2209
TseFI GTSAC 1 cut(s) 112
TseI GCWGC 4 cut(s) 1229, 1945, 2134, 2275
Tsp45I GTSAC 1 cut(s) 112
TspDTI ATGAA 7 cut(s) 332, 582, 584, 804, 872, 1269, 1935
TspRI CASTG 6 cut(s) 187, 1213, 1500, 1614, 2125, 2209
VpaK11BI GGWCC 2 cut(s) 1064, 1790
VspI ATTAAT 1 cut(s) 1164
XagI CCTNNNNNAGG 1 cut(s) 1230
XapI RAATTY 9 cut(s) 338, 560, 683, 919, 1589, 1965, 2314, 2348, 2467
XceI RCATGY 3 cut(s) 656, 1984, 2154
XmiI GTMKAC 2 cut(s) 2214, 2452
XmnI GAANNNNTTC 1 cut(s) 1165
ZrmI AGTACT 1 cut(s) 457
Zsp2I ATGCAT 3 cut(s) 32, 422, 2035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.