RLG00000031455

May participate in a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001097
Physical Location & Seq
Reverse (-)
5363025 .. 5370974
7950 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031455

Sequence Viewer

Length: 2451 bp
ATGGCGAAGCGTGCGTACAAGCTGCAGGAGTTTGTGGCGCATTCTGACAATATTAACTGTCTAAATATTGGGAAAAAGGCGTGCCGACTTTATGTCACCGGTGGTGACGATCACAAAGTCAATCTATGGACAATTGGCAAACCTACGGCGTTAATGAGTCTCACTGGTTATACAAGTCCAGTTGAATCTGTAGCTTTTAATTCAGCAGAAGTTTTGGTGCTTGCTGGAGCTTCTACTGGTGCAACAAAAATATGGGATCTGGAGGAAACAAAGATGGTTCGCACACTTACCGGACACAGATCCAGCTGTTCTGCTGTTGAATTTCATCCATTTGGTGAGTTCTTTGCTTCTGGTTCCATGGACACTAATCTGAAGATCTGGGACATTAGAAAGAAGGGATGCATCCACACATACAAAGGTCATACTCAAGGCATTAGTACTATTAAGTTCACTCCCGATGGTCGATGGGTAGTTTCTGGTGGGTTTGACAATGTTGTAAAGGTGTGGGATCTAACTGCTGGGAAACTCTTGCATGATTTTAAATTCCATGAAGGCCATATTCGGTCTATAGATTTTCATCCCCTTGAGTTTCTCTTGGCCACAGGTTCAGCAGACAGAACTGTGAAATTCTGGGATCTGGAAACTTTTGAACTGATTGGATCTACCAGGCCTGAGCCTACAGGAGTACGCGCAATTACTTTCCACCCTGATGGGAGGACAATATTTTCTGGATTGGATGACAGTTTGAAGGTTTATTCATGGGAGCCTGTAATATCCCATGATTCTGTTGATGTGGGATGGTCGAGACTTGCTGACTTATGCATTCATGACGGGAAGCTATTGGGATGCTCTTACCATCGAAATTCTATTGGAGTCTGGGTTGCAGATGTGTCGCTTATTAAGCCATATGGAGCTGGTTCAACACCTGAGCAAAATGACTGTTTAGATGAGAAATCTCATGTAAAGTTGCAAGGAATCATGGAGAAGGTAGAGACTGGTATAAGGGGTCCAGGTTTCCGCTGTACGTCTCCTGATTATGACACAAAGGAAATAAAGAACATATATGTTGATTCTGCAGGTGGAAAGCCAGTTACCTTACAGAGAATTAATTCTTCAAAAGTTTTACCCCCATTGGATTTAAAGCAAACCAGTGCTAAGAAGCAGATTCCTGCAGCAGGGTCGCTCCCAAAGTCTAATGAAAAGACGGTCAATAAATCCCTGGTTGTGCCTAGCATCGTACCTCGAAGCAGTCCCAATGCAAAGGATTCAGCTAACTCTGGAAGAGAATCTGTTGCATTTTCGAAAACTAGGCGTGGTATGTTGCTCAGACCAGCTCATGTGCGAAGCCCATCAAATAGCAAACTTGACGTGGAGAAGCTACCGTTGGCCGTTGAATCTGAAAGTTTCAGCAGTATGACGAGTAAATTGGATGGTGCAGTGGGTCTCAGTTCTCAAATCAAAACTGTATCAGAAGATAGTGATAGAGAGGCCTGTGAGGAAAAACGTTCTGCAATCAAGAGTGTAGAAGAGAAATTTGAAGCAATATTATCACTGAAGAGGCCCTCTGATCAGGAAACCAGAAATGGATCCCTCAATAACAGCAAGGAAGTGAGCCCCGTTAAAGTTGTCAATGGAGTTGCCGTTGTACCAGGAAGGACTCGCACCCTGGTTGAGAGATTTGAAAGGAGAGAGAGATTTAGTAGCAATGACGATCAAGCAACTGGCGTACTTGGTCCCGTGATACCTGAATATCAAGCAACCAGCATAACTCGTACTGTGATACCCGAAGATCAAGCAAGTAGCATATCTAGTCCTGTGATGCCTGAAGATCAAGCAGCTAGCATATGTAGTCCCGTGATACATGAAATGGATAAAACATTCACCATGCAGCGAGAAGAAGAACCTCAAATTTCTGGGAGGCACATGTCATCTGCAAACAATGATCCTGCTGAAGATCTGATGCAAAGTCATGATGCATTCCTAAGTACCCTTCGATCACGCCTGACAAAACTGCAGGTGGTGAGGCATTTTTGGGAACGAAATGATATTAAAGGTGCTATTTGTTCTATCAGAAAGCTGCCAGATCATTCGGTACAAGCAGATGTGATTAGTGTTCTCCTGGAGAAAATGGATATTCTGACATTAGATCTCTTTTCATGTTTGCTGCCTGTGCTTCTGGGCTTACTTGATAGCAAAATTGAGAGGCATGCAAATGTGTCATTGGAGATGCTGTTGAAGCTAGTAGCAGTCTTTGGCCCAGTGATACGGTCTACCATTTCAGCACCTCCAGCGGTCGGAGTCAATATTCAGGCAGAGCAAAGGCTGGAGTGCTGCAACCAATGCTCTGTGCACCTGCAAAAGATCCAGAAAATTCTCCCAGTCTTTGTAAGGAAGGGGGGTGTGCTGGCAAGATGTGCTCTAGAGTTGAATCTAATTCTTCAAGAGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Katanin_con80 WD40

Protein Analysis

817

Amino Acids

89.8

Weight (kDa)

7.84

Isoelectric Point (pI)

47.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 8 - 137 1.9e-24 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 8 - 129 1.2e-08 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 9 - 129 1.8e-19 WDR3 first beta-propeller domain
WD40_Gbeta PF25391 10 - 132 4.4e-13 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_1st PF25171 12 - 127 2.1e-07 WDR36/Utp21 first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 12 - 136 2.1e-11 WDR36/Utp21 second beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 13 - 171 1.8e-09 MABP1/WDR62 second WD40 domain
WD40_Prp19 PF24814 15 - 253 2.2e-44 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 22 - 140 2.6e-17 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 29 - 177 4.5e-18 Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz PF24807 50 - 245 6.2e-32 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_2nd PF25172 51 - 173 3.1e-17 WDR3 second beta-propeller domain
EIF3I PF24805 52 - 138 7.8e-06 EIF3I
Beta-prop_SPT8 PF23798 58 - 152 2.3e-06 SPT8 beta propeller
Beta-prop_THOC3 PF25174 58 - 200 1.6e-31 THOC3 beta-propeller domain
WDR55 PF24796 60 - 256 4.9e-17 WDR55
WD40 PF00400 91 - 128 1.9e-09 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 95 - 251 1.5e-36 WDR5 beta-propeller domain
Beta-prop_Aladin PF25460 101 - 173 1.1e-07 Aladin seven-bladed propeller
Beta-prop_WDR3_1st PF25173 105 - 253 3.4e-30 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 118 - 252 2e-11 WDR36/Utp21 first beta-propeller
WD40_Gbeta PF25391 120 - 255 1.3e-15 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 128 - 201 2.4e-11 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 134 - 170 8e-12 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 136 - 219 1.2e-10 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 136 - 254 3.9e-06 TEP-1 second beta-propeller
Beta-prop_EML PF23409 138 - 241 5.3e-07 Echinoderm microtubule-associated protein first beta-propeller
EIF3I PF24805 139 - 250 2.1e-14 EIF3I
WD40_WDHD1_1st PF24817 142 - 218 3.7e-10 WDHD1 first WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 163 - 245 1.9e-09 WDR90/POC16, second beta-propeller
Beta-prop_THOC3 PF25174 164 - 248 1.9e-13 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 167 - 217 3.4e-06 WDR3 second beta-propeller domain
WD40 PF00400 174 - 212 5.6e-08 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 189 - 293 5.3e-07 Echinoderm microtubule-associated protein second beta-propeller
Katanin_con80 PF13925 655 - 812 2.4e-52 con80 domain of Katanin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 1067, 2005, 2361
Acc36I ACCTGC 3 cut(s) 1067, 2005, 2361
AccI GTMKAC 1 cut(s) 2270
AccII CGCG 1 cut(s) 690
AciI CCGC 2 cut(s) 1018, 2291
AclI AACGTT 1 cut(s) 1504
AclWI GGATC 9 cut(s) 264, 294, 516, 642, 667, 1581, 1594, 1937, 2356
AcoI YGGCCR 2 cut(s) 597, 1386
AcsI RAATTY 7 cut(s) 320, 542, 626, 862, 1532, 1908, 2370
AcuI CTGAAG 4 cut(s) 392, 1574, 1845, 1971
AfiI CCNNNNNNNGG 2 cut(s) 1175, 2294
AflIII ACRYGT 1 cut(s) 1923
AgeI ACCGGT 1 cut(s) 98
AhdI GACNNNNNGTC 1 cut(s) 92
AjiI CACGTC 1 cut(s) 1369
AjnI CCWGG 6 cut(s) 665, 1009, 1218, 1648, 1665, 2118
AjuI GAANNNNNNNTTGG 2 cut(s) 1367, 1399
Alw21I GWGCWC 2 cut(s) 2352, 2419
Alw26I GTCTC 5 cut(s) 164, 799, 986, 1032, 1448
Alw44I GTGCAC 1 cut(s) 2348
AlwI GGATC 9 cut(s) 264, 294, 516, 642, 667, 1581, 1594, 1937, 2356
AoxI GGCC 7 cut(s) 553, 597, 668, 1386, 1488, 1559, 2254
ApaLI GTGCAC 1 cut(s) 2348
ApeKI GCWGC 7 cut(s) 22, 1172, 1835, 1888, 2077, 2164, 2331
ApoI RAATTY 7 cut(s) 320, 542, 626, 862, 1532, 1908, 2370
AseI ATTAAT 1 cut(s) 1107
AsiGI ACCGGT 1 cut(s) 98
Asp700I GAANNNNTTC 1 cut(s) 1108
AspLEI GCGC 2 cut(s) 40, 692
AspS9I GGNCC 4 cut(s) 1007, 1560, 1733, 2255
AsuHPI GGTGA 5 cut(s) 88, 116, 347, 1873, 2032
AsuII TTCGAA 1 cut(s) 1301
AsuNHI GCTAGC 1 cut(s) 1838
AvaII GGWCC 2 cut(s) 1007, 1733
BaeGI GKGCMC 1 cut(s) 2352
BalI TGGCCA 1 cut(s) 599
BamHI GGATCC 1 cut(s) 1586
BanII GRGCYC 1 cut(s) 1616
BarI GAAGNNNNNNTAC 1 cut(s) 31
Bbv12I GWGCWC 2 cut(s) 2352, 2419
BbvI GCAGC 7 cut(s) 9, 1184, 1847, 1900, 2064, 2151, 2318
BccI CCATC 8 cut(s) 268, 452, 459, 704, 792, 864, 1357, 1424
BceAI ACGGC 3 cut(s) 162, 1373, 1625
BcgI CGANNNNNNTGC 4 cut(s) 873, 907, 1161, 1195
BciT130I CCWGG 6 cut(s) 667, 1011, 1220, 1650, 1667, 2120
BclI TGATCA 1 cut(s) 1567
BcoDI GTCTC 5 cut(s) 164, 799, 986, 1032, 1448
BfaI CTAG 6 cut(s) 1230, 1308, 1809, 1839, 2240, 2420
BfmI CTRYAG 7 cut(s) 23, 189, 567, 678, 1074, 1170, 2012
BfuAI ACCTGC 3 cut(s) 1067, 2005, 2361
BglII AGATCT 3 cut(s) 375, 1954, 2146
BisI GCNGC 7 cut(s) 23, 1173, 1836, 1889, 2078, 2165, 2332
BlsI GCNGC 7 cut(s) 24, 1174, 1837, 1890, 2079, 2166, 2333
BmcAI AGTACT 1 cut(s) 439
Bme1390I CCNGG 6 cut(s) 667, 1011, 1220, 1650, 1667, 2120
Bme18I GGWCC 2 cut(s) 1007, 1733
BmeRI GACNNNNNGTC 1 cut(s) 92
BmgBI CACGTC 1 cut(s) 1369
BmgT120I GGNCC 4 cut(s) 1007, 1560, 1733, 2255
BmiI GGNNCC 5 cut(s) 355, 765, 1008, 1588, 1735
BmrFI CCNGG 6 cut(s) 667, 1011, 1220, 1650, 1667, 2120
BmrI ACTGGG 2 cut(s) 2252, 2372
BmsI GCATC 8 cut(s) 389, 411, 836, 1242, 1809, 1950, 1963, 2217
BmtI GCTAGC 1 cut(s) 1842
BmuI ACTGGG 2 cut(s) 2252, 2372
BpmI CTGGAG 5 cut(s) 246, 281, 2141, 2271, 2345
Bpu10I CCTNAGC 2 cut(s) 672, 927
Bpu14I TTCGAA 1 cut(s) 1301
BpuEI CTTGAG 2 cut(s) 411, 605
BsaBI GATNNNNATC 1 cut(s) 576
BsaI GGTCTC 1 cut(s) 1448
BsaJI CCNNGG 3 cut(s) 357, 1218, 1665
BsaWI WCCGGW 2 cut(s) 98, 290
BsaXI ACNNNNNCTCC 6 cut(s) 864, 894, 1626, 1656, 2216, 2246
Bsc4I CCNNNNNNNGG 2 cut(s) 1175, 2294
Bse118I RCCGGY 1 cut(s) 98
Bse1I ACTGG 9 cut(s) 169, 179, 241, 1000, 1088, 1149, 1726, 2258, 2378
Bse3DI GCAATG 1 cut(s) 1711
Bse8I GATNNNNATC 1 cut(s) 576
BseBI CCWGG 6 cut(s) 667, 1011, 1220, 1650, 1667, 2120
BseDI CCNNGG 3 cut(s) 357, 1218, 1665
BseGI GGATG 8 cut(s) 325, 402, 404, 577, 742, 803, 851, 1435
BseJI GATNNNNATC 1 cut(s) 576
BseLI CCNNNNNNNGG 2 cut(s) 1175, 2294
BseMI GCAATG 1 cut(s) 1711
BseMII CTCAG 4 cut(s) 663, 918, 1339, 1459
BseNI ACTGG 9 cut(s) 169, 179, 241, 1000, 1088, 1149, 1726, 2258, 2378
BseSI GKGCMC 1 cut(s) 2352
BseXI GCAGC 7 cut(s) 9, 1184, 1847, 1900, 2064, 2151, 2318
BseYI CCCAGC 1 cut(s) 518
BsgI GTGCAG 1 cut(s) 1455
Bsh1236I CGCG 1 cut(s) 690
Bsh1285I CGRYCG 1 cut(s) 2295
BshFI GGCC 7 cut(s) 555, 599, 670, 1388, 1490, 1561, 2256
BshTI ACCGGT 1 cut(s) 98
BsiEI CGRYCG 1 cut(s) 2295
BsiHKAI GWGCWC 2 cut(s) 2352, 2419
BsiSI CCGG 2 cut(s) 99, 291
BslFI GGGAC 4 cut(s) 395, 1236, 1719, 1836
BslI CCNNNNNNNGG 2 cut(s) 1175, 2294
BsmAI GTCTC 5 cut(s) 164, 799, 986, 1032, 1448
BsmBI CGTCTC 1 cut(s) 1032
BsmFI GGGAC 4 cut(s) 395, 1236, 1719, 1836
BsmI GAATGC 3 cut(s) 40, 822, 1976
BsnI GGCC 7 cut(s) 555, 599, 670, 1388, 1490, 1561, 2256
Bso31I GGTCTC 1 cut(s) 1448
Bsp119I TTCGAA 1 cut(s) 1301
Bsp1286I GDGCHC 3 cut(s) 1616, 2352, 2419
Bsp19I CCATGG 1 cut(s) 357
BspACI CCGC 2 cut(s) 1018, 2291
BspANI GGCC 7 cut(s) 555, 599, 670, 1388, 1490, 1561, 2256
BspCNI CTCAG 4 cut(s) 664, 919, 1338, 1458
BspFNI CGCG 1 cut(s) 690
BspHI TCATGA 2 cut(s) 826, 1969
BspLI GGNNCC 5 cut(s) 355, 765, 1008, 1588, 1735
BspMAI CTGCAG 4 cut(s) 27, 1078, 1174, 2016
BspMI ACCTGC 3 cut(s) 1067, 2005, 2361
BspOI GCTAGC 1 cut(s) 1842
BspPI GGATC 9 cut(s) 264, 294, 516, 642, 667, 1581, 1594, 1937, 2356
BspT104I TTCGAA 1 cut(s) 1301
BspTNI GGTCTC 1 cut(s) 1448
BsrDI GCAATG 1 cut(s) 1711
BsrFI RCCGGY 1 cut(s) 98
BsrI ACTGG 9 cut(s) 169, 179, 241, 1000, 1088, 1149, 1726, 2258, 2378
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 3 cut(s) 357, 1218, 1665
BssT1I CCWWGG 1 cut(s) 357
Bst2UI CCWGG 6 cut(s) 667, 1011, 1220, 1650, 1667, 2120
Bst4CI ACNGT 9 cut(s) 59, 622, 743, 941, 1207, 1383, 1465, 1777, 2268
Bst6I CTCTTC 3 cut(s) 1276, 1521, 1550
BstAPI GCANNNNNTGC 2 cut(s) 2340, 2414
BstBI TTCGAA 1 cut(s) 1301
BstC8I GCNNGC 6 cut(s) 12, 82, 222, 1840, 2208, 2406
BstDEI CTNAG 6 cut(s) 672, 927, 1155, 1325, 1445, 1982
BstDSI CCRYGG 1 cut(s) 357
BstENI CCTNNNNNAGG 1 cut(s) 1173
BstF5I GGATG 8 cut(s) 325, 402, 404, 577, 742, 803, 851, 1435
BstFNI CGCG 1 cut(s) 690
BstHHI GCGC 2 cut(s) 40, 692
BstMAI GTCTC 5 cut(s) 164, 799, 986, 1032, 1448
BstMCI CGRYCG 1 cut(s) 2295
BstMWI GCNNNNNNNGC 7 cut(s) 11, 901, 2170, 2236, 2288, 2340, 2414
BstNI CCWGG 6 cut(s) 667, 1011, 1220, 1650, 1667, 2120
BstNSI RCATGY 2 cut(s) 1927, 2210
BstSCI CCNGG 6 cut(s) 665, 1009, 1218, 1648, 1665, 2118
BstSFI CTRYAG 7 cut(s) 23, 189, 567, 678, 1074, 1170, 2012
BstSLI GKGCMC 1 cut(s) 2352
BstUI CGCG 1 cut(s) 690
BstV1I GCAGC 7 cut(s) 9, 1184, 1847, 1900, 2064, 2151, 2318
BstXI CCANNNNNNTGG 1 cut(s) 710
BsuRI GGCC 7 cut(s) 555, 599, 670, 1388, 1490, 1561, 2256
BtgI CCRYGG 1 cut(s) 357
BtrI CACGTC 1 cut(s) 1369
BtsCI GGATG 8 cut(s) 325, 402, 404, 577, 742, 803, 851, 1435
BtsI GCAGTG 1 cut(s) 1443
BtsIMutI CAGTG 5 cut(s) 162, 1156, 1443, 1550, 2265
BveI ACCTGC 3 cut(s) 1067, 2005, 2361
Cac8I GCNNGC 6 cut(s) 12, 82, 222, 1840, 2208, 2406
CciI TCATGA 2 cut(s) 826, 1969
CfoI GCGC 2 cut(s) 40, 692
Cfr10I RCCGGY 1 cut(s) 98
Cfr13I GGNCC 4 cut(s) 1007, 1560, 1733, 2255
CspAI ACCGGT 1 cut(s) 98
DdeI CTNAG 6 cut(s) 672, 927, 1155, 1325, 1445, 1982
DraI TTTAAA 2 cut(s) 541, 1140
DriI GACNNNNNGTC 1 cut(s) 92
EaeI YGGCCR 2 cut(s) 597, 1386
Eam1104I CTCTTC 3 cut(s) 1276, 1521, 1550
Eam1105I GACNNNNNGTC 1 cut(s) 92
EarI CTCTTC 3 cut(s) 1276, 1521, 1550
Eco130I CCWWGG 1 cut(s) 357
Eco147I AGGCCT 2 cut(s) 670, 1490
Eco24I GRGCYC 1 cut(s) 1616
Eco31I GGTCTC 1 cut(s) 1448
Eco47I GGWCC 2 cut(s) 1007, 1733
Eco57I CTGAAG 4 cut(s) 392, 1574, 1845, 1971
EcoNI CCTNNNNNAGG 1 cut(s) 1173
EcoO109I RGGNCCY 1 cut(s) 1560
EcoRII CCWGG 6 cut(s) 665, 1009, 1218, 1648, 1665, 2118
EcoT14I CCWWGG 1 cut(s) 357
EcoT22I ATGCAT 3 cut(s) 404, 824, 1978
EcoT38I GRGCYC 1 cut(s) 1616
ErhI CCWWGG 1 cut(s) 357
Esp3I CGTCTC 1 cut(s) 1032
FaqI GGGAC 4 cut(s) 395, 1236, 1719, 1836
FauNDI CATATG 2 cut(s) 907, 1844
FbaI TGATCA 1 cut(s) 1567
FblI GTMKAC 1 cut(s) 2270
Fnu4HI GCNGC 7 cut(s) 23, 1173, 1836, 1889, 2078, 2165, 2332
FokI GGATG 8 cut(s) 312, 389, 411, 564, 749, 810, 858, 1442
FriOI GRGCYC 1 cut(s) 1616
Fsp4HI GCNGC 7 cut(s) 23, 1173, 1836, 1889, 2078, 2165, 2332
FspBI CTAG 6 cut(s) 1230, 1308, 1809, 1839, 2240, 2420
GlaI GCGC 2 cut(s) 39, 691
GluI GCNGC 7 cut(s) 23, 1173, 1836, 1889, 2078, 2165, 2332
GsaI CCCAGC 1 cut(s) 522
GsuI CTGGAG 5 cut(s) 246, 281, 2141, 2271, 2345
HaeIII GGCC 7 cut(s) 555, 599, 670, 1388, 1490, 1561, 2256
HapII CCGG 2 cut(s) 99, 291
HhaI GCGC 2 cut(s) 40, 692
Hin6I GCGC 2 cut(s) 38, 690
HinP1I GCGC 2 cut(s) 38, 690
HpaII CCGG 2 cut(s) 99, 291
HphI GGTGA 5 cut(s) 88, 116, 347, 1873, 2032
Hpy166II GTNNAC 3 cut(s) 450, 2271, 2350
Hpy8I GTNNAC 3 cut(s) 450, 2271, 2350
HpyAV CCTTC 7 cut(s) 388, 545, 742, 979, 1647, 2000, 2386
HpyCH4III ACNGT 9 cut(s) 59, 622, 743, 941, 1207, 1383, 1465, 1777, 2268
HpyCH4IV ACGT 3 cut(s) 1025, 1368, 1504
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 901, 2170, 2236, 2288, 2340, 2414
HpyF3I CTNAG 6 cut(s) 672, 927, 1155, 1325, 1445, 1982
HpySE526I ACGT 3 cut(s) 1025, 1368, 1504
HspAI GCGC 2 cut(s) 38, 690
Ksp22I TGATCA 1 cut(s) 1567
LmnI GCTCC 4 cut(s) 227, 763, 911, 1188
Lsp1109I GCAGC 7 cut(s) 9, 1184, 1847, 1900, 2064, 2151, 2318
LweI GCATC 8 cut(s) 389, 411, 836, 1242, 1809, 1950, 1963, 2217
MaeI CTAG 6 cut(s) 1230, 1308, 1809, 1839, 2240, 2420
MaeII ACGT 3 cut(s) 1025, 1368, 1504
MaeIII GTNAC 3 cut(s) 94, 104, 1090
MfeI CAATTG 1 cut(s) 132
MhlI GDGCHC 3 cut(s) 1616, 2352, 2419
MlsI TGGCCA 1 cut(s) 599
MluNI TGGCCA 1 cut(s) 599
MlyI GAGTC 4 cut(s) 166, 882, 1651, 2307
MmeI TCCRAC 1 cut(s) 2275
Mox20I TGGCCA 1 cut(s) 599
Mph1103I ATGCAT 3 cut(s) 404, 824, 1978
MroXI GAANNNNTTC 1 cut(s) 1108
MscI TGGCCA 1 cut(s) 599
MslI CAYNNNNRTG 2 cut(s) 708, 2211
Msp20I TGGCCA 1 cut(s) 599
MspA1I CMGCKG 3 cut(s) 306, 1020, 2291
MspI CCGG 2 cut(s) 99, 291
MspR9I CCNGG 6 cut(s) 667, 1011, 1220, 1650, 1667, 2120
MunI CAATTG 1 cut(s) 132
Mva1269I GAATGC 3 cut(s) 40, 822, 1976
MvaI CCWGG 6 cut(s) 667, 1011, 1220, 1650, 1667, 2120
MvnI CGCG 1 cut(s) 690
MwoI GCNNNNNNNGC 7 cut(s) 11, 901, 2170, 2236, 2288, 2340, 2414
NcoI CCATGG 1 cut(s) 357
NdeI CATATG 2 cut(s) 907, 1844
NheI GCTAGC 1 cut(s) 1838
NlaIV GGNNCC 5 cut(s) 355, 765, 1008, 1588, 1735
NmuCI GTSAC 2 cut(s) 94, 104
NsiI ATGCAT 3 cut(s) 404, 824, 1978
NspI RCATGY 2 cut(s) 1927, 2210
NspV TTCGAA 1 cut(s) 1301
PaeI GCATGC 1 cut(s) 2210
PagI TCATGA 2 cut(s) 826, 1969
PaqCI CACCTGC 3 cut(s) 1067, 2005, 2361
PceI AGGCCT 2 cut(s) 670, 1490
PciI ACATGT 1 cut(s) 1923
PctI GAATGC 3 cut(s) 40, 822, 1976
PdmI GAANNNNTTC 1 cut(s) 1108
PfeI GAWTC 9 cut(s) 185, 782, 975, 1070, 1165, 1265, 1286, 1394, 2428
PfoI TCCNGGA 1 cut(s) 2118
PinAI ACCGGT 1 cut(s) 98
PkrI GCNGC 7 cut(s) 24, 1174, 1837, 1890, 2079, 2166, 2333
PleI GAGTC 4 cut(s) 165, 881, 1651, 2306
PpsI GAGTC 4 cut(s) 165, 881, 1651, 2306
PscI ACATGT 1 cut(s) 1923
PshBI ATTAAT 1 cut(s) 1107
Psp1406I AACGTT 1 cut(s) 1504
Psp6I CCWGG 6 cut(s) 665, 1009, 1218, 1648, 1665, 2118
PspFI CCCAGC 1 cut(s) 518
PspGI CCWGG 6 cut(s) 665, 1009, 1218, 1648, 1665, 2118
PspN4I GGNNCC 5 cut(s) 355, 765, 1008, 1588, 1735
PspPI GGNCC 4 cut(s) 1007, 1560, 1733, 2255
PstI CTGCAG 4 cut(s) 27, 1078, 1174, 2016
PvuII CAGCTG 1 cut(s) 306
RseI CAYNNNNRTG 2 cut(s) 708, 2211
SatI GCNGC 7 cut(s) 23, 1173, 1836, 1889, 2078, 2165, 2332
Sau96I GGNCC 4 cut(s) 1007, 1560, 1733, 2255
ScaI AGTACT 1 cut(s) 439
SchI GAGTC 4 cut(s) 166, 882, 1651, 2307
ScrFI CCNGG 6 cut(s) 667, 1011, 1220, 1650, 1667, 2120
SduI GDGCHC 3 cut(s) 1616, 2352, 2419
SfaNI GCATC 8 cut(s) 389, 411, 836, 1242, 1809, 1950, 1963, 2217
SfcI CTRYAG 7 cut(s) 23, 189, 567, 678, 1074, 1170, 2012
SfuI TTCGAA 1 cut(s) 1301
SgrAI CRCCGGYG 1 cut(s) 98
SinI GGWCC 2 cut(s) 1007, 1733
SmiMI CAYNNNNRTG 2 cut(s) 708, 2211
SmlI CTYRAG 2 cut(s) 426, 584
SmoI CTYRAG 2 cut(s) 426, 584
SphI GCATGC 1 cut(s) 2210
SseBI AGGCCT 2 cut(s) 670, 1490
SsiI CCGC 2 cut(s) 1018, 2291
SspI AATATT 5 cut(s) 52, 67, 723, 1545, 2305
SspMI CTAG 6 cut(s) 1230, 1308, 1809, 1839, 2240, 2420
StuI AGGCCT 2 cut(s) 670, 1490
StyD4I CCNGG 6 cut(s) 665, 1009, 1218, 1648, 1665, 2118
StyI CCWWGG 1 cut(s) 357
TaaI ACNGT 9 cut(s) 59, 622, 743, 941, 1207, 1383, 1465, 1777, 2268
TaiI ACGT 3 cut(s) 1028, 1371, 1507
TaqI TCGA 6 cut(s) 463, 803, 859, 1243, 1301, 1993
TaqII GACCGA 1 cut(s) 552
TatI WGTACW 1 cut(s) 437
TfiI GAWTC 9 cut(s) 185, 782, 975, 1070, 1165, 1265, 1286, 1394, 2428
TscAI CASTG 5 cut(s) 169, 1156, 1443, 1557, 2265
TseFI GTSAC 2 cut(s) 94, 104
TseI GCWGC 7 cut(s) 22, 1172, 1835, 1888, 2077, 2164, 2331
Tsp45I GTSAC 2 cut(s) 94, 104
TspDTI ATGAA 8 cut(s) 314, 564, 566, 747, 815, 1212, 1878, 2145
TspRI CASTG 5 cut(s) 169, 1156, 1443, 1557, 2265
VneI GTGCAC 1 cut(s) 2348
VpaK11BI GGWCC 2 cut(s) 1007, 1733
VspI ATTAAT 1 cut(s) 1107
XagI CCTNNNNNAGG 1 cut(s) 1173
XapI RAATTY 7 cut(s) 320, 542, 626, 862, 1532, 1908, 2370
XbaI TCTAGA 1 cut(s) 2419
XceI RCATGY 2 cut(s) 1927, 2210
XmiI GTMKAC 1 cut(s) 2270
XmnI GAANNNNTTC 1 cut(s) 1108
XspI CTAG 6 cut(s) 1230, 1308, 1809, 1839, 2240, 2420
ZrmI AGTACT 1 cut(s) 439
Zsp2I ATGCAT 3 cut(s) 404, 824, 1978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.