RH5BG052700

May participate in a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0001097
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG052700.1

Sequence Viewer

Length: 2451 bp
ATGGCGAAGCGTGCATACAAGCTGCAGGAGTTTGCGGCGCATTCTGACAATATTAACTGTCTAAATATTGGGAAAAAGGCGTGCCGACTTTATGTCACCGGTGGTGACGATCGCAAAGTCAATCTATGGACAATTGGCAAACCTACTGCGTTAATGAGTCTCACTGGTCATACAAGTCCAGTTGAATCTGTGGCTTTTAATTCGGCAGAAGTTTTGGTTCTTGCTGGAGCTTCTACTGGTGCAACAAAAATATGGGATCTGGAGGAAACAAAGATGGTTCGCACACTTACTGGACACAGATCCAGCTGTTCTGCTGTTGAATTTCATCCATTTGGTGAGTTCTTTGCTTCTGGTTCCATGGACACTAATCTGAAGATCTGGGACATTAGAAAGAAGGGATGCATCCACACATACAAAGGTCATACTCAAGGCATTAGTACTATTAAGTTCACTCCCGATGGTCGATGGGTAGTTTCTGGTGGGTTTGACAATGTTGTAAAGGTTTGGGATCTAACTGCTGGGAAGCTCTTGCATGATTTTAAATTCCATGAAGGACATATTCGGTCTTTAGATTTTCATCCCCTTGAGTTTCTCTTGGCCACAGGTTCAGCAGACAGAACTGTGAAATTCTGGGATCTGGAAACTTTTGAATTGATTGGATCTACCAGGCCTGAGCCTACAGGAGTACGCGCAATTACTTTCCACCCTGATGGGAGGACAATATTTTCTGGATTGGATGACAGTTTGAAGGTTTATTCATGGGAGCCTGTGATATCCCATGATTCTGTTGATATGGGATGGTCAAGACTAGCTGACTTATGCATTCATGACGGGAAGCTATTGGGATGCTCTTACTATCGAAATTCTATTGGAGTCTGGGTTGCGGATGTGTCGCTTATTAAGCCATATGGAGCTGGTTCAACACCTGAGCAAAATGACTGTTTAGATGAGAAATCTCATGTGAAGTTGCAAGGAATCATGGAGAAGGTAGAGACTGGTATAAGGGGGCCGGGTTTGCGCTGTATGTCTCCTGATTATGACACAAAGGAAATAAAGAACATATATATTGATTCTGCAGGTGGAAAGCCAGTTACCTCACAGAGAATTAATTCTTCAAAAGTTTTACCCCCATTGGATTTAAAGGAAACCAGTGCTAAGAAGCAGATTCCTGCAGCAGGGTCACTCCCAAAGTCTAATGAAAAGATGGTCAATAAATCCCTGGTTGTGCCTAGCATCGTACCTCGAAGCAGTCCCAATGCAAAAGACTCAGCTAACTCTGGGAGAGATTCTGTTGCATTTTCGAAAACTAGGCGTGGTATGTTGGTCAGACCAGCTCATGTGCGAAGCCCATCAAATAGCAAAGTTGACGTGGAGAAGCTACCGTTGGCCGTTGAATCTGAAACTTTCAGCAGTATGACGAGTAAATTGGATGGTGCAGTGGGTCTCAGTTCTCAAATCAAAACTGCATCAGAAGATAGTGATAGAGAGGCCTGTACGGAAAAACATTCTGCAATCAAGAGTGTAGAAGAGAAATTTGAAGCAGTATTATCACTGAAGAGGCCCTCTGATCAGGAAACCAGAAATGGATCCCTCAATAACAGCAAGGAAGTGAGCCCTGTTAAAGTTGTCAATGGAGTTGCCGTTGTACCAGGAAGGACTCGCACCCTGGTTGAGAGATTTGAAAGGAGAGAGAGATTTAGTAGCAATGACGATCAAGCAACTGGCATATTTGGTCCCGTGATACCGGAATATCACGCAACCAGCATATCTCGTACTGTGATACCTGAAGATCAAGCAAGTAGCATATCTAGTCCTGTGATGCCTGAAGATCAAGCAACTAGCATATGTAGTCCCGTGATACATGAAATGGATAAAACATTCACCATGCAGCGAGAAGAAGAACCTCAAACTTCTGGGAGGCACATGTCATCTGCAAACAATGATCCTGCTGAAGATCTGATGCAAACTCATGATGCATTCCTAAGTACCCTTCGATCCCGCCTGACAAAACTGCAGGTGGTGAGGCATTTTTGGGAACGAAATGATATTAAAGGTGCTATCAGTGCTATCAGAAAGCTGCCAGATCATTCGGTACAAGCAGATGTGATTAGTGTTCTCCTGGAGAAAATGGATATTCTGACATTAGATCTCTTTTCGTGTTTGCTGCCTGTGCTTCTGGGCTTACTTGATAGCAAAATTGAGAGGCATGCAAATGTGTCATTGGAGATGCTGTTGAAGCTAGTAGCAGTCTTTGGCCCAGTGATACGGTCTACCATTTCAGCACCTCCAGCGGTCGGAGTCAATCTTCAGGCAGAGCAAAGGCTGGAGTGCTGCAACCAATGCTCTGTACACCTGCAAAAGATCCAGAAAATTCTCCCAGTCTTTGTAAGGAAGGGGGGCGTGCTGGCAAGATGTGCTCTAGAATTGAATCTAATTCTTCAAGAGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Katanin_con80 WD40

Protein Analysis

816

Amino Acids

89.77

Weight (kDa)

7.85

Isoelectric Point (pI)

45.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 7 - 69 5.4e-06 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 8 - 137 1.1e-25 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 8 - 129 2.9e-10 TEP-1 second beta-propeller
WD40_Gbeta PF25391 9 - 132 2.4e-14 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_1st PF25171 9 - 127 8.8e-08 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 9 - 129 2.3e-21 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 11 - 135 9.8e-12 WDR36/Utp21 second beta-propeller domain
WD40_Prp19 PF24814 14 - 254 8.6e-46 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 26 - 141 3.9e-16 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 29 - 177 4.6e-18 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 51 - 173 1.8e-18 WDR3 second beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 52 - 137 2e-06 CAF1B/HIR1 beta-propeller domain
EIF3I PF24805 52 - 138 4.9e-07 EIF3I
Beta-prop_SPT8 PF23798 57 - 152 1.4e-06 SPT8 beta propeller
Beta-prop_THOC3 PF25174 57 - 200 1.5e-31 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 60 - 245 4.1e-31 CDC20/Fizzy WD40 domain
WDR55 PF24796 60 - 255 8.4e-17 WDR55
WD40_MABP1-WDR62_2nd PF24782 81 - 171 1.1e-08 MABP1/WDR62 second WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 81 - 160 1.3e-07 WDR90/POC16, second beta-propeller
WD40 PF00400 91 - 128 1.9e-09 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 95 - 251 2e-36 WDR5 beta-propeller domain
Beta-prop_Aladin PF25460 101 - 195 5.2e-08 Aladin seven-bladed propeller
Beta-prop_WDR3_1st PF25173 105 - 253 3.6e-30 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 115 - 187 5.1e-06 TEP-1 second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 118 - 252 2.7e-11 WDR36/Utp21 first beta-propeller
WD40_Gbeta PF25391 119 - 255 2e-15 G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd PF25168 128 - 201 1.8e-11 WDR36/Utp21 second beta-propeller domain
WD40 PF00400 134 - 170 8e-12 WD domain, G-beta repeat
Beta-prop_TEP1_2nd PF25047 136 - 254 6.6e-06 TEP-1 second beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 136 - 219 2.9e-10 CAF1B/HIR1 beta-propeller domain
Beta-prop_EML PF23409 138 - 241 4.2e-07 Echinoderm microtubule-associated protein first beta-propeller
EIF3I PF24805 139 - 250 6.1e-14 EIF3I
Beta-prop_THOC3 PF25174 141 - 248 7e-20 THOC3 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 142 - 214 5.5e-06 MABP1/WDR62 second WD40 domain
WD40_WDHD1_1st PF24817 143 - 218 7.3e-10 WDHD1 first WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 163 - 245 6.8e-09 WDR90/POC16, second beta-propeller
Beta-prop_WDR3_2nd PF25172 167 - 217 4.9e-06 WDR3 second beta-propeller domain
WD40 PF00400 174 - 212 4.9e-08 WD domain, G-beta repeat
Beta-prop_EML_2 PF23414 189 - 247 6.7e-07 Echinoderm microtubule-associated protein second beta-propeller
Katanin_con80 PF13925 655 - 812 3.7e-53 con80 domain of Katanin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 1067, 2005, 2361
Acc36I ACCTGC 3 cut(s) 1067, 2005, 2361
AccI GTMKAC 1 cut(s) 2270
AccII CGCG 1 cut(s) 690
AciI CCGC 4 cut(s) 35, 884, 1999, 2291
AcoI YGGCCR 2 cut(s) 597, 1386
AcsI RAATTY 6 cut(s) 320, 542, 626, 862, 1532, 2370
AcuI CTGAAG 6 cut(s) 392, 1574, 1806, 1845, 1971, 2291
AfaI GTAC 9 cut(s) 439, 687, 1239, 1495, 1647, 1774, 1987, 2094, 2349
AfiI CCNNNNNNNGG 2 cut(s) 1175, 2294
AflIII ACRYGT 1 cut(s) 1923
AgeI ACCGGT 1 cut(s) 98
AhdI GACNNNNNGTC 1 cut(s) 92
AjiI CACGTC 1 cut(s) 1369
AjnI CCWGG 5 cut(s) 665, 1218, 1648, 1665, 2118
AjuI GAANNNNNNNTTGG 2 cut(s) 1367, 1399
Alw21I GWGCWC 1 cut(s) 2419
Alw26I GTCTC 4 cut(s) 164, 986, 1032, 1448
AoxI GGCC 7 cut(s) 597, 668, 1007, 1386, 1488, 1559, 2254
ApeKI GCWGC 6 cut(s) 22, 1172, 1888, 2077, 2164, 2331
ApoI RAATTY 6 cut(s) 320, 542, 626, 862, 1532, 2370
AseI ATTAAT 1 cut(s) 1107
AsiGI ACCGGT 1 cut(s) 98
Asp700I GAANNNNTTC 1 cut(s) 1108
AspLEI GCGC 3 cut(s) 40, 692, 1020
AspS9I GGNCC 4 cut(s) 1007, 1560, 1733, 2255
AsuC2I CCSGG 1 cut(s) 1011
AsuHPI GGTGA 5 cut(s) 88, 116, 347, 1873, 2032
AsuII TTCGAA 1 cut(s) 1301
AvaII GGWCC 1 cut(s) 1733
BalI TGGCCA 1 cut(s) 599
BamHI GGATCC 1 cut(s) 1586
BanII GRGCYC 1 cut(s) 1616
BarI GAAGNNNNNNTAC 1 cut(s) 31
Bbv12I GWGCWC 1 cut(s) 2419
BbvI GCAGC 6 cut(s) 9, 1184, 1900, 2064, 2151, 2318
BccI CCATC 8 cut(s) 268, 452, 459, 704, 792, 1198, 1357, 1424
BceAI ACGGC 2 cut(s) 1373, 1625
BciT130I CCWGG 5 cut(s) 667, 1220, 1650, 1667, 2120
BclI TGATCA 1 cut(s) 1567
BcnI CCSGG 1 cut(s) 1011
BcoDI GTCTC 4 cut(s) 164, 986, 1032, 1448
BfaI CTAG 7 cut(s) 809, 1230, 1308, 1809, 1839, 2240, 2420
BfmI CTRYAG 5 cut(s) 23, 678, 1074, 1170, 2012
BfuAI ACCTGC 3 cut(s) 1067, 2005, 2361
BglII AGATCT 3 cut(s) 375, 1954, 2146
BisI GCNGC 7 cut(s) 23, 36, 1173, 1889, 2078, 2165, 2332
BlsI GCNGC 7 cut(s) 24, 37, 1174, 1890, 2079, 2166, 2333
BmcAI AGTACT 1 cut(s) 439
Bme1390I CCNGG 6 cut(s) 667, 1011, 1220, 1650, 1667, 2120
Bme18I GGWCC 1 cut(s) 1733
BmeRI GACNNNNNGTC 1 cut(s) 92
BmgBI CACGTC 1 cut(s) 1369
BmgT120I GGNCC 4 cut(s) 1007, 1560, 1733, 2255
BmiI GGNNCC 5 cut(s) 355, 765, 1008, 1588, 1735
BmrFI CCNGG 6 cut(s) 667, 1011, 1220, 1650, 1667, 2120
BmrI ACTGGG 2 cut(s) 2252, 2372
BmsI GCATC 9 cut(s) 389, 411, 836, 1242, 1475, 1809, 1950, 1963, 2217
BmuI ACTGGG 2 cut(s) 2252, 2372
BpmI CTGGAG 5 cut(s) 246, 281, 2141, 2271, 2345
Bpu10I CCTNAGC 2 cut(s) 672, 927
Bpu14I TTCGAA 1 cut(s) 1301
BpuEI CTTGAG 2 cut(s) 411, 605
BpuMI CCSGG 1 cut(s) 1011
BsaBI GATNNNNATC 1 cut(s) 576
BsaI GGTCTC 1 cut(s) 1448
BsaJI CCNNGG 3 cut(s) 357, 1218, 1665
BsaWI WCCGGW 2 cut(s) 98, 1744
BsaXI ACNNNNNCTCC 6 cut(s) 864, 894, 1626, 1656, 2216, 2246
Bsc4I CCNNNNNNNGG 2 cut(s) 1175, 2294
Bse118I RCCGGY 1 cut(s) 98
Bse3DI GCAATG 1 cut(s) 1711
Bse8I GATNNNNATC 1 cut(s) 576
BseBI CCWGG 5 cut(s) 667, 1220, 1650, 1667, 2120
BseDI CCNNGG 3 cut(s) 357, 1218, 1665
BseGI GGATG 9 cut(s) 325, 402, 404, 577, 742, 803, 851, 892, 1435
BseJI GATNNNNATC 1 cut(s) 576
BseLI CCNNNNNNNGG 2 cut(s) 1175, 2294
BseMI GCAATG 1 cut(s) 1711
BseMII CTCAG 4 cut(s) 663, 918, 1281, 1459
BseXI GCAGC 6 cut(s) 9, 1184, 1900, 2064, 2151, 2318
BseYI CCCAGC 1 cut(s) 518
BsgI GTGCAG 1 cut(s) 1455
Bsh1236I CGCG 1 cut(s) 690
Bsh1285I CGRYCG 2 cut(s) 112, 2295
BshFI GGCC 7 cut(s) 599, 670, 1009, 1388, 1490, 1561, 2256
BshTI ACCGGT 1 cut(s) 98
BsiEI CGRYCG 2 cut(s) 112, 2295
BsiHKAI GWGCWC 1 cut(s) 2419
BsiSI CCGG 3 cut(s) 99, 1010, 1745
BslFI GGGAC 4 cut(s) 395, 1236, 1719, 1836
BslI CCNNNNNNNGG 2 cut(s) 1175, 2294
BsmAI GTCTC 4 cut(s) 164, 986, 1032, 1448
BsmFI GGGAC 4 cut(s) 395, 1236, 1719, 1836
BsmI GAATGC 3 cut(s) 40, 822, 1976
BsnI GGCC 7 cut(s) 599, 670, 1009, 1388, 1490, 1561, 2256
Bso31I GGTCTC 1 cut(s) 1448
Bsp119I TTCGAA 1 cut(s) 1301
Bsp1286I GDGCHC 2 cut(s) 1616, 2419
Bsp1407I TGTACA 1 cut(s) 2347
Bsp19I CCATGG 1 cut(s) 357
BspACI CCGC 4 cut(s) 35, 884, 1999, 2291
BspANI GGCC 7 cut(s) 599, 670, 1009, 1388, 1490, 1561, 2256
BspCNI CTCAG 4 cut(s) 664, 919, 1280, 1458
BspFNI CGCG 1 cut(s) 690
BspHI TCATGA 2 cut(s) 826, 1969
BspLI GGNNCC 5 cut(s) 355, 765, 1008, 1588, 1735
BspMAI CTGCAG 4 cut(s) 27, 1078, 1174, 2016
BspMI ACCTGC 3 cut(s) 1067, 2005, 2361
BspT104I TTCGAA 1 cut(s) 1301
BspTNI GGTCTC 1 cut(s) 1448
BsrDI GCAATG 1 cut(s) 1711
BsrFI RCCGGY 1 cut(s) 98
BsrGI TGTACA 1 cut(s) 2347
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 3 cut(s) 357, 1218, 1665
BssT1I CCWWGG 1 cut(s) 357
Bst2UI CCWGG 5 cut(s) 667, 1220, 1650, 1667, 2120
Bst4CI ACNGT 7 cut(s) 59, 622, 743, 941, 1383, 1777, 2268
Bst6I CTCTTC 2 cut(s) 1521, 1550
BstAPI GCANNNNNTGC 2 cut(s) 2340, 2414
BstAUI TGTACA 1 cut(s) 2347
BstBI TTCGAA 1 cut(s) 1301
BstC8I GCNNGC 5 cut(s) 12, 82, 2208, 2402, 2406
BstDEI CTNAG 6 cut(s) 672, 927, 1155, 1267, 1445, 1982
BstDSI CCRYGG 1 cut(s) 357
BstENI CCTNNNNNAGG 1 cut(s) 1173
BstF5I GGATG 9 cut(s) 325, 402, 404, 577, 742, 803, 851, 892, 1435
BstFNI CGCG 1 cut(s) 690
BstHHI GCGC 3 cut(s) 40, 692, 1020
BstMAI GTCTC 4 cut(s) 164, 986, 1032, 1448
BstMCI CGRYCG 2 cut(s) 112, 2295
BstMWI GCNNNNNNNGC 9 cut(s) 11, 901, 1015, 2063, 2170, 2236, 2288, 2340, 2414
BstNI CCWGG 5 cut(s) 667, 1220, 1650, 1667, 2120
BstNSI RCATGY 2 cut(s) 1927, 2210
BstSCI CCNGG 6 cut(s) 665, 1009, 1218, 1648, 1665, 2118
BstSFI CTRYAG 5 cut(s) 23, 678, 1074, 1170, 2012
BstUI CGCG 1 cut(s) 690
BstV1I GCAGC 6 cut(s) 9, 1184, 1900, 2064, 2151, 2318
BstXI CCANNNNNNTGG 1 cut(s) 710
BsuRI GGCC 7 cut(s) 599, 670, 1009, 1388, 1490, 1561, 2256
BtgI CCRYGG 1 cut(s) 357
BtrI CACGTC 1 cut(s) 1369
BtsCI GGATG 9 cut(s) 325, 402, 404, 577, 742, 803, 851, 892, 1435
BtsI GCAGTG 1 cut(s) 1443
BtsIMutI CAGTG 6 cut(s) 162, 1156, 1443, 1550, 2068, 2265
BveI ACCTGC 3 cut(s) 1067, 2005, 2361
Cac8I GCNNGC 5 cut(s) 12, 82, 2208, 2402, 2406
CciI TCATGA 2 cut(s) 826, 1969
CfoI GCGC 3 cut(s) 40, 692, 1020
Cfr10I RCCGGY 1 cut(s) 98
Cfr13I GGNCC 4 cut(s) 1007, 1560, 1733, 2255
Csp6I GTAC 9 cut(s) 438, 686, 1238, 1494, 1646, 1773, 1986, 2093, 2348
CspAI ACCGGT 1 cut(s) 98
CviQI GTAC 9 cut(s) 438, 686, 1238, 1494, 1646, 1773, 1986, 2093, 2348
DdeI CTNAG 6 cut(s) 672, 927, 1155, 1267, 1445, 1982
DraI TTTAAA 2 cut(s) 541, 1140
DriI GACNNNNNGTC 1 cut(s) 92
EaeI YGGCCR 2 cut(s) 597, 1386
Eam1104I CTCTTC 2 cut(s) 1521, 1550
Eam1105I GACNNNNNGTC 1 cut(s) 92
EarI CTCTTC 2 cut(s) 1521, 1550
Eco130I CCWWGG 1 cut(s) 357
Eco147I AGGCCT 2 cut(s) 670, 1490
Eco24I GRGCYC 1 cut(s) 1616
Eco31I GGTCTC 1 cut(s) 1448
Eco32I GATATC 1 cut(s) 774
Eco47I GGWCC 1 cut(s) 1733
Eco57I CTGAAG 6 cut(s) 392, 1574, 1806, 1845, 1971, 2291
EcoNI CCTNNNNNAGG 1 cut(s) 1173
EcoO109I RGGNCCY 1 cut(s) 1560
EcoRII CCWGG 5 cut(s) 665, 1218, 1648, 1665, 2118
EcoRV GATATC 1 cut(s) 774
EcoT14I CCWWGG 1 cut(s) 357
EcoT22I ATGCAT 3 cut(s) 404, 824, 1978
EcoT38I GRGCYC 1 cut(s) 1616
ErhI CCWWGG 1 cut(s) 357
FaqI GGGAC 4 cut(s) 395, 1236, 1719, 1836
FauI CCCGC 1 cut(s) 2006
FauNDI CATATG 2 cut(s) 907, 1844
FbaI TGATCA 1 cut(s) 1567
FblI GTMKAC 1 cut(s) 2270
Fnu4HI GCNGC 7 cut(s) 23, 36, 1173, 1889, 2078, 2165, 2332
FokI GGATG 9 cut(s) 312, 389, 411, 564, 749, 810, 858, 899, 1442
FriOI GRGCYC 1 cut(s) 1616
Fsp4HI GCNGC 7 cut(s) 23, 36, 1173, 1889, 2078, 2165, 2332
FspBI CTAG 7 cut(s) 809, 1230, 1308, 1809, 1839, 2240, 2420
GlaI GCGC 3 cut(s) 39, 691, 1019
GluI GCNGC 7 cut(s) 23, 36, 1173, 1889, 2078, 2165, 2332
GsaI CCCAGC 1 cut(s) 522
GsuI CTGGAG 5 cut(s) 246, 281, 2141, 2271, 2345
HaeIII GGCC 7 cut(s) 599, 670, 1009, 1388, 1490, 1561, 2256
HapII CCGG 3 cut(s) 99, 1010, 1745
HhaI GCGC 3 cut(s) 40, 692, 1020
Hin6I GCGC 3 cut(s) 38, 690, 1018
HinP1I GCGC 3 cut(s) 38, 690, 1018
HincII GTYRAC 1 cut(s) 1366
HindII GTYRAC 1 cut(s) 1366
HpaII CCGG 3 cut(s) 99, 1010, 1745
HphI GGTGA 5 cut(s) 88, 116, 347, 1873, 2032
Hpy166II GTNNAC 4 cut(s) 450, 1366, 2271, 2350
Hpy8I GTNNAC 4 cut(s) 450, 1366, 2271, 2350
HpyAV CCTTC 7 cut(s) 388, 545, 742, 979, 1647, 2000, 2386
HpyCH4III ACNGT 7 cut(s) 59, 622, 743, 941, 1383, 1777, 2268
HpyCH4IV ACGT 1 cut(s) 1368
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 901, 1015, 2063, 2170, 2236, 2288, 2340, 2414
HpyF3I CTNAG 6 cut(s) 672, 927, 1155, 1267, 1445, 1982
HpySE526I ACGT 1 cut(s) 1368
HspAI GCGC 3 cut(s) 38, 690, 1018
Ksp22I TGATCA 1 cut(s) 1567
LmnI GCTCC 3 cut(s) 227, 763, 911
Lsp1109I GCAGC 6 cut(s) 9, 1184, 1900, 2064, 2151, 2318
LweI GCATC 9 cut(s) 389, 411, 836, 1242, 1475, 1809, 1950, 1963, 2217
MaeI CTAG 7 cut(s) 809, 1230, 1308, 1809, 1839, 2240, 2420
MaeII ACGT 1 cut(s) 1368
MaeIII GTNAC 4 cut(s) 94, 104, 1090, 1179
MfeI CAATTG 1 cut(s) 132
MhlI GDGCHC 2 cut(s) 1616, 2419
MlsI TGGCCA 1 cut(s) 599
MluNI TGGCCA 1 cut(s) 599
MlyI GAGTC 5 cut(s) 166, 882, 1259, 1651, 2307
MmeI TCCRAC 1 cut(s) 2275
Mox20I TGGCCA 1 cut(s) 599
Mph1103I ATGCAT 3 cut(s) 404, 824, 1978
MroXI GAANNNNTTC 1 cut(s) 1108
MscI TGGCCA 1 cut(s) 599
MslI CAYNNNNRTG 2 cut(s) 708, 2211
Msp20I TGGCCA 1 cut(s) 599
MspA1I CMGCKG 2 cut(s) 306, 2291
MspI CCGG 3 cut(s) 99, 1010, 1745
MspR9I CCNGG 6 cut(s) 667, 1011, 1220, 1650, 1667, 2120
MunI CAATTG 1 cut(s) 132
Mva1269I GAATGC 3 cut(s) 40, 822, 1976
MvaI CCWGG 5 cut(s) 667, 1220, 1650, 1667, 2120
MvnI CGCG 1 cut(s) 690
MwoI GCNNNNNNNGC 9 cut(s) 11, 901, 1015, 2063, 2170, 2236, 2288, 2340, 2414
NciI CCSGG 1 cut(s) 1011
NcoI CCATGG 1 cut(s) 357
NdeI CATATG 2 cut(s) 907, 1844
NlaIV GGNNCC 5 cut(s) 355, 765, 1008, 1588, 1735
NmuCI GTSAC 3 cut(s) 94, 104, 1179
NsiI ATGCAT 3 cut(s) 404, 824, 1978
NspI RCATGY 2 cut(s) 1927, 2210
NspV TTCGAA 1 cut(s) 1301
PaeI GCATGC 1 cut(s) 2210
PagI TCATGA 2 cut(s) 826, 1969
PaqCI CACCTGC 3 cut(s) 1067, 2005, 2361
PceI AGGCCT 2 cut(s) 670, 1490
PciI ACATGT 1 cut(s) 1923
PctI GAATGC 3 cut(s) 40, 822, 1976
PdmI GAANNNNTTC 1 cut(s) 1108
PfeI GAWTC 8 cut(s) 185, 782, 975, 1070, 1165, 1286, 1394, 2428
PfoI TCCNGGA 1 cut(s) 2118
PinAI ACCGGT 1 cut(s) 98
PkrI GCNGC 7 cut(s) 24, 37, 1174, 1890, 2079, 2166, 2333
Ple19I CGATCG 1 cut(s) 112
PleI GAGTC 5 cut(s) 165, 881, 1259, 1651, 2306
PpsI GAGTC 5 cut(s) 165, 881, 1259, 1651, 2306
PscI ACATGT 1 cut(s) 1923
PshBI ATTAAT 1 cut(s) 1107
Psp6I CCWGG 5 cut(s) 665, 1218, 1648, 1665, 2118
PspFI CCCAGC 1 cut(s) 518
PspGI CCWGG 5 cut(s) 665, 1218, 1648, 1665, 2118
PspN4I GGNNCC 5 cut(s) 355, 765, 1008, 1588, 1735
PspPI GGNCC 4 cut(s) 1007, 1560, 1733, 2255
PstI CTGCAG 4 cut(s) 27, 1078, 1174, 2016
PvuI CGATCG 1 cut(s) 112
PvuII CAGCTG 1 cut(s) 306
RsaI GTAC 9 cut(s) 439, 687, 1239, 1495, 1647, 1774, 1987, 2094, 2349
RsaNI GTAC 9 cut(s) 438, 686, 1238, 1494, 1646, 1773, 1986, 2093, 2348
RseI CAYNNNNRTG 2 cut(s) 708, 2211
SatI GCNGC 7 cut(s) 23, 36, 1173, 1889, 2078, 2165, 2332
Sau96I GGNCC 4 cut(s) 1007, 1560, 1733, 2255
ScaI AGTACT 1 cut(s) 439
SchI GAGTC 5 cut(s) 166, 882, 1259, 1651, 2307
ScrFI CCNGG 6 cut(s) 667, 1011, 1220, 1650, 1667, 2120
SduI GDGCHC 2 cut(s) 1616, 2419
SfaNI GCATC 9 cut(s) 389, 411, 836, 1242, 1475, 1809, 1950, 1963, 2217
SfcI CTRYAG 5 cut(s) 23, 678, 1074, 1170, 2012
SfuI TTCGAA 1 cut(s) 1301
SgrAI CRCCGGYG 1 cut(s) 98
SinI GGWCC 1 cut(s) 1733
SmiMI CAYNNNNRTG 2 cut(s) 708, 2211
SmlI CTYRAG 2 cut(s) 426, 584
SmoI CTYRAG 2 cut(s) 426, 584
SphI GCATGC 1 cut(s) 2210
SseBI AGGCCT 2 cut(s) 670, 1490
SsiI CCGC 4 cut(s) 35, 884, 1999, 2291
SspI AATATT 3 cut(s) 52, 67, 723
SspMI CTAG 7 cut(s) 809, 1230, 1308, 1809, 1839, 2240, 2420
StuI AGGCCT 2 cut(s) 670, 1490
StyD4I CCNGG 6 cut(s) 665, 1009, 1218, 1648, 1665, 2118
StyI CCWWGG 1 cut(s) 357
TaaI ACNGT 7 cut(s) 59, 622, 743, 941, 1383, 1777, 2268
TaiI ACGT 1 cut(s) 1371
TaqI TCGA 5 cut(s) 463, 859, 1243, 1301, 1993
TaqII GACCGA 1 cut(s) 552
TatI WGTACW 2 cut(s) 437, 2347
TauI GCSGC 1 cut(s) 38
TfiI GAWTC 8 cut(s) 185, 782, 975, 1070, 1165, 1286, 1394, 2428
TscAI CASTG 6 cut(s) 169, 1156, 1443, 1557, 2068, 2265
TseFI GTSAC 3 cut(s) 94, 104, 1179
TseI GCWGC 6 cut(s) 22, 1172, 1888, 2077, 2164, 2331
Tsp45I GTSAC 3 cut(s) 94, 104, 1179
TspDTI ATGAA 7 cut(s) 314, 564, 566, 747, 815, 1212, 1878
TspGWI ACGGA 1 cut(s) 1511
TspRI CASTG 6 cut(s) 169, 1156, 1443, 1557, 2068, 2265
VpaK11BI GGWCC 1 cut(s) 1733
VspI ATTAAT 1 cut(s) 1107
XagI CCTNNNNNAGG 1 cut(s) 1173
XapI RAATTY 6 cut(s) 320, 542, 626, 862, 1532, 2370
XbaI TCTAGA 1 cut(s) 2419
XceI RCATGY 2 cut(s) 1927, 2210
XmiI GTMKAC 1 cut(s) 2270
XmnI GAANNNNTTC 1 cut(s) 1108
XspI CTAG 7 cut(s) 809, 1230, 1308, 1809, 1839, 2240, 2420
ZrmI AGTACT 1 cut(s) 439
Zsp2I ATGCAT 3 cut(s) 404, 824, 1978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.