Rorug04G0426700

May participate in a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001097
Physical Location & Seq
Forward (+)
58555854 .. 58558153
2300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0426700.1

Sequence Viewer

Length: 1623 bp
ATGCACAAGCTTTTCAAAACCGGCCGTGGAATTCTCTATCCCATCCTCCTCAGAAACCCCAAATTCCTGACACCCATTTCACAATTTCACATCTCCAGCGAAACCCTAGCTCCCAATCGGAAGCTCGAATTCGTAGTCAATGAAGTTCAACAGCTTCGATCATCAAATCCCAGTAATGAATTTGTCTCGGCCTCAGACACCGACGAACCGTCCGGCCCGGCCAACGTGGAAGGTTTTGCGGTCCAGATTTCGCATCCATGGCGGGAATGGGTGGACCTGATGGAGCTTTTGCTGAAGAGAGGCTACTTTGAAGGAGACGAAAACCCGTTTCGGAACGGCCAAGTTGGTCCAAAGGAATCGAACCGTATCCGAACCGCTTGCCTTAATTTTGCTCGGGAAAGGTCGAGTCTTCTCAGCTTATTGTCGAGGAAAGATGTCCAGATCATTGCAGGGTATGGGTGCCCAAGCATAGACAGGAAAGTTGTCAACTCAGGAAAGCGGCTAAGGGCACATGTCGGGGTTGATGAAGGAGATGTGTGCAGCTCCTGCGGTTTGAGAGGCGACTGTGAGAGAGCATATGTAAAGGCCCGTGAAGATGAAGGTGGACGTACTGTGGATGTCATGCGATTCTTGTTAACACATGGGCTTGATCCCCTTACTGATACAGTGGGGAACAAGCCTTCTCTAAATAAAAAGGTCAAAGAATCAGTGAGAAGGCTATTGAAAGAGATGGTGGAGTACAGCACTGATAAGCTTGATACCGATCTCCCAACGAACATGACTTTAAAAAGGGTTAAACCCTCGCCAAAACATGCAATGCCACAAGAAAAGTACAACATAAATGTTCCAATGAAGCAAGGCGATTGGATCTGCCCCAAATGCAACTTCCTCAATTTTGCCAGAAATGTTAAGTGCTTACGTTGTGATGGTCTATTCCAAGAAAGACTGGCGAAACTAAGGGAGGATCAGGATCATCTTCCATTGAAGAAGGGGGACTGGATATGTGAAAAATGCAATTTCCTAAATTTTGCCAAGAACAGTAGATGCTTGCAGTGCAAAGAGAAGCCACCCAAGCGAGAGCTAAATCCTGGAGAGTGGGAGTGTGACTCCTGCAATTACATCAATTTTAGGAAAAACATGCTATGCTTAAAATGTGATCATAAAAGGCCCAAGACTTCTGCTGAACCTATACATGAAGATGGAGGCTACCTTAACAGTAATAGTGTTAGTTTTGGTGACACTGATGTTAGTGATCGATCCTACTTAGGACAAGGTAGAAATGGTCAAAACAGAGGTGCAGGCAGGTGGAGATTTGTGGTTGAAGAGAATGATGATGATAGCCACTCAAACTCATTAGATAAGACTTCCAGGTTTGTAGACTTCCCAATTGCTGGAGGCAAGACTGAACTGTCTCAGAATCCACAAAAGAGGAACAGATGGAAGTTGAAGATGTTAGAGAAGAGCAAAGGAGGCACAATGGATATGGCAAGTGATGATGAACTTGGGTCTACCAGTAATGAAAGTAGAGTATTGTTTTCTGATTCTACGGATGATGAAGAGATGGCTGAGTGGTTTGGGGATAGAAAGCTTGAAACACAAAGGCTTCCATCTAGGCCTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Katanin_con80 WD40

Protein Analysis

540

Amino Acids

61.5

Weight (kDa)

8.62

Isoelectric Point (pI)

42.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_RanBP PF00641 285 - 310 2.1e-08 Zn-finger in Ran binding protein and others
Zn_ribbon_RanBP PF00641 329 - 353 3.4e-06 Zn-finger in Ran binding protein and others
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1293
AasI GACNNNNNNGTC 1 cut(s) 1408
Acc36I ACCTGC 1 cut(s) 1293
AccB1I GGYRCC 1 cut(s) 459
AccB7I CCANNNNNTGG 1 cut(s) 1391
AccI GTMKAC 2 cut(s) 1377, 1508
AciI CCGC 5 cut(s) 239, 262, 375, 499, 549
AclWI GGATC 5 cut(s) 644, 875, 972, 978, 1251
AcoI YGGCCR 3 cut(s) 22, 219, 337
AcsI RAATTY 5 cut(s) 30, 62, 128, 179, 1024
AcuI CTGAAG 1 cut(s) 314
AfaI GTAC 3 cut(s) 610, 740, 833
AfiI CCNNNNNNNGG 1 cut(s) 1391
AflIII ACRYGT 1 cut(s) 511
AgsI TTSAA 8 cut(s) 16, 149, 311, 724, 985, 1322, 1447, 1592
AhdI GACNNNNNGTC 1 cut(s) 208
AjnI CCWGG 2 cut(s) 1087, 1367
AjuI GAANNNNNNNTTGG 2 cut(s) 869, 901
Alw26I GTCTC 3 cut(s) 190, 309, 1416
AlwI GGATC 5 cut(s) 644, 875, 972, 978, 1251
AlwNI CAGNNNCTG 1 cut(s) 546
Ama87I CYCGRG 1 cut(s) 393
AoxI GGCC 8 cut(s) 22, 189, 214, 219, 337, 585, 1166, 1613
ApeKI GCWGC 1 cut(s) 540
ApoI RAATTY 5 cut(s) 30, 62, 128, 179, 1024
ArsI GACNNNNNNTTYG 2 cut(s) 120, 152
AspS9I GGNCC 6 cut(s) 215, 241, 274, 347, 586, 1167
AsuC2I CCSGG 1 cut(s) 218
AsuHPI GGTGA 1 cut(s) 1247
AvaI CYCGRG 1 cut(s) 393
AvaII GGWCC 3 cut(s) 241, 274, 347
BaeGI GKGCMC 2 cut(s) 464, 511
BanI GGYRCC 1 cut(s) 459
BarI GAAGNNNNNNTAC 2 cut(s) 287, 319
BbsI GAAGAC 1 cut(s) 401
BbvI GCAGC 1 cut(s) 552
BccI CCATC 8 cut(s) 50, 274, 724, 920, 1193, 1431, 1555, 1615
BceAI ACGGC 2 cut(s) 9, 352
BcgI CGANNNNNNTGC 2 cut(s) 360, 394
BciT130I CCWGG 2 cut(s) 1089, 1369
BciVI GTATCC 1 cut(s) 377
BclI TGATCA 1 cut(s) 1156
BcnI CCSGG 1 cut(s) 218
BcoDI GTCTC 3 cut(s) 190, 309, 1416
BfaI CTAG 2 cut(s) 107, 1611
BfuAI ACCTGC 1 cut(s) 1293
BfuI GTATCC 1 cut(s) 377
BisI GCNGC 2 cut(s) 500, 541
BlsI GCNGC 2 cut(s) 501, 542
Bme1390I CCNGG 3 cut(s) 218, 1089, 1369
Bme18I GGWCC 3 cut(s) 241, 274, 347
BmeRI GACNNNNNGTC 1 cut(s) 208
BmeT110I CYCGRG 1 cut(s) 393
BmgT120I GGNCC 6 cut(s) 215, 241, 274, 347, 586, 1167
BmiI GGNNCC 1 cut(s) 461
BmrFI CCNGG 3 cut(s) 218, 1089, 1369
BmrI ACTGGG 1 cut(s) 165
BmsI GCATC 2 cut(s) 262, 1034
BmuI ACTGGG 1 cut(s) 165
BpiI GAAGAC 1 cut(s) 401
BplI GAGNNNNNCTC 2 cut(s) 1091, 1123
BpmI CTGGAG 3 cut(s) 79, 1110, 1413
Bpu10I CCTNAGC 1 cut(s) 503
BpuMI CCSGG 1 cut(s) 218
Bsa29I ATCGAT 1 cut(s) 1255
BsaBI GATNNNNATC 2 cut(s) 762, 969
BsaJI CCNNGG 2 cut(s) 25, 257
BsaXI ACNNNNNCTCC 2 cut(s) 94, 124
Bsc4I CCNNNNNNNGG 1 cut(s) 1391
Bse118I RCCGGY 1 cut(s) 20
Bse1I ACTGG 4 cut(s) 171, 951, 1001, 1512
Bse3DI GCAATG 2 cut(s) 444, 822
Bse8I GATNNNNATC 2 cut(s) 762, 969
BseBI CCWGG 2 cut(s) 1089, 1369
BseCI ATCGAT 1 cut(s) 1255
BseDI CCNNGG 2 cut(s) 25, 257
BseGI GGATG 4 cut(s) 42, 253, 622, 1555
BseJI GATNNNNATC 2 cut(s) 762, 969
BseLI CCNNNNNNNGG 1 cut(s) 1391
BseMI GCAATG 2 cut(s) 444, 822
BseMII CTCAG 6 cut(s) 64, 207, 427, 504, 1427, 1557
BseNI ACTGG 4 cut(s) 171, 951, 1001, 1512
BseRI GAGGAG 1 cut(s) 38
BseSI GKGCMC 2 cut(s) 464, 511
BseX3I CGGCCG 1 cut(s) 22
BseXI GCAGC 1 cut(s) 552
BsgI GTGCAG 2 cut(s) 559, 1317
Bsh1285I CGRYCG 1 cut(s) 25
BshFI GGCC 8 cut(s) 24, 191, 216, 221, 339, 587, 1168, 1615
BshNI GGYRCC 1 cut(s) 459
BshVI ATCGAT 1 cut(s) 1255
BsiEI CGRYCG 1 cut(s) 25
BsiHKCI CYCGRG 1 cut(s) 393
BsiSI CCGG 3 cut(s) 21, 213, 218
BslFI GGGAC 1 cut(s) 1007
BslI CCNNNNNNNGG 1 cut(s) 1391
BsmAI GTCTC 3 cut(s) 190, 309, 1416
BsmBI CGTCTC 1 cut(s) 309
BsmFI GGGAC 1 cut(s) 1007
BsnI GGCC 8 cut(s) 24, 191, 216, 221, 339, 587, 1168, 1615
BsoBI CYCGRG 1 cut(s) 393
Bsp1286I GDGCHC 2 cut(s) 464, 511
Bsp19I CCATGG 1 cut(s) 257
BspACI CCGC 5 cut(s) 239, 262, 375, 499, 549
BspANI GGCC 8 cut(s) 24, 191, 216, 221, 339, 587, 1168, 1615
BspCNI CTCAG 6 cut(s) 63, 206, 426, 503, 1426, 1558
BspDI ATCGAT 1 cut(s) 1255
BspLI GGNNCC 1 cut(s) 461
BspMI ACCTGC 1 cut(s) 1293
BspPI GGATC 5 cut(s) 644, 875, 972, 978, 1251
BspQI GCTCTTC 1 cut(s) 1454
BspT107I GGYRCC 1 cut(s) 459
BsrDI GCAATG 2 cut(s) 444, 822
BsrFI RCCGGY 1 cut(s) 20
BsrI ACTGG 4 cut(s) 171, 951, 1001, 1512
BssAI RCCGGY 1 cut(s) 20
BssECI CCNNGG 2 cut(s) 25, 257
BssT1I CCWWGG 1 cut(s) 257
Bst2UI CCWGG 2 cut(s) 1089, 1369
Bst4CI ACNGT 8 cut(s) 210, 365, 566, 613, 667, 1040, 1217, 1410
Bst6I CTCTTC 4 cut(s) 290, 1317, 1454, 1551
BstAPI GCANNNNNTGC 1 cut(s) 546
BstC8I GCNNGC 3 cut(s) 379, 1049, 1300
BstDEI CTNAG 9 cut(s) 50, 193, 413, 490, 503, 956, 1264, 1413, 1566
BstDSI CCRYGG 2 cut(s) 25, 257
BstF5I GGATG 4 cut(s) 42, 253, 622, 1555
BstMAI GTCTC 3 cut(s) 190, 309, 1416
BstMCI CGRYCG 1 cut(s) 25
BstMWI GCNNNNNNNGC 6 cut(s) 259, 546, 879, 1053, 1072, 1470
BstNI CCWGG 2 cut(s) 1089, 1369
BstNSI RCATGY 3 cut(s) 515, 815, 1141
BstSCI CCNGG 3 cut(s) 216, 1087, 1367
BstSLI GKGCMC 2 cut(s) 464, 511
BstV1I GCAGC 1 cut(s) 552
BstV2I GAAGAC 1 cut(s) 401
BstX2I RGATCY 1 cut(s) 867
BstYI RGATCY 1 cut(s) 867
BstZI CGGCCG 1 cut(s) 22
Bsu15I ATCGAT 1 cut(s) 1255
BsuI GTATCC 1 cut(s) 377
BsuRI GGCC 8 cut(s) 24, 191, 216, 221, 339, 587, 1168, 1615
BsuTUI ATCGAT 1 cut(s) 1255
BtgI CCRYGG 2 cut(s) 25, 257
BtsCI GGATG 4 cut(s) 42, 253, 622, 1555
BtsI GCAGTG 1 cut(s) 1058
BtsIMutI CAGTG 5 cut(s) 672, 714, 744, 1058, 1239
BveI ACCTGC 1 cut(s) 1293
Cac8I GCNNGC 3 cut(s) 379, 1049, 1300
CaiI CAGNNNCTG 1 cut(s) 546
Cfr10I RCCGGY 1 cut(s) 20
Cfr13I GGNCC 6 cut(s) 215, 241, 274, 347, 586, 1167
ClaI ATCGAT 1 cut(s) 1255
Csp6I GTAC 3 cut(s) 609, 739, 832
CviAII CATG 8 cut(s) 258, 512, 622, 641, 778, 812, 1138, 1193
CviQI GTAC 3 cut(s) 609, 739, 832
DdeI CTNAG 9 cut(s) 50, 193, 413, 490, 503, 956, 1264, 1413, 1566
DraI TTTAAA 1 cut(s) 786
DrdI GACNNNNNNGTC 1 cut(s) 1408
DriI GACNNNNNGTC 1 cut(s) 208
DseDI GACNNNNNNGTC 1 cut(s) 1408
EaeI YGGCCR 3 cut(s) 22, 219, 337
EagI CGGCCG 1 cut(s) 22
Eam1104I CTCTTC 4 cut(s) 290, 1317, 1454, 1551
Eam1105I GACNNNNNGTC 1 cut(s) 208
EarI CTCTTC 4 cut(s) 290, 1317, 1454, 1551
EclXI CGGCCG 1 cut(s) 22
Eco130I CCWWGG 1 cut(s) 257
Eco147I AGGCCT 1 cut(s) 1615
Eco47I GGWCC 3 cut(s) 241, 274, 347
Eco52I CGGCCG 1 cut(s) 22
Eco57I CTGAAG 1 cut(s) 314
Eco88I CYCGRG 1 cut(s) 393
EcoRI GAATTC 2 cut(s) 30, 128
EcoRII CCWGG 2 cut(s) 1087, 1367
EcoT14I CCWWGG 1 cut(s) 257
ErhI CCWWGG 1 cut(s) 257
Esp3I CGTCTC 1 cut(s) 309
FaeI CATG 8 cut(s) 261, 515, 625, 644, 781, 815, 1141, 1196
FaqI GGGAC 1 cut(s) 1007
FatI CATG 8 cut(s) 257, 511, 621, 640, 777, 811, 1137, 1192
FauI CCCGC 1 cut(s) 255
FauNDI CATATG 1 cut(s) 577
FbaI TGATCA 1 cut(s) 1156
FblI GTMKAC 2 cut(s) 1377, 1508
Fnu4HI GCNGC 2 cut(s) 500, 541
FokI GGATG 4 cut(s) 29, 240, 629, 1562
Fsp4HI GCNGC 2 cut(s) 500, 541
FspBI CTAG 2 cut(s) 107, 1611
GluI GCNGC 2 cut(s) 500, 541
GsuI CTGGAG 3 cut(s) 79, 1110, 1413
HaeIII GGCC 8 cut(s) 24, 191, 216, 221, 339, 587, 1168, 1615
HapII CCGG 3 cut(s) 21, 213, 218
Hin1II CATG 8 cut(s) 261, 515, 625, 644, 781, 815, 1141, 1196
HincII GTYRAC 2 cut(s) 487, 636
HindII GTYRAC 2 cut(s) 487, 636
HindIII AAGCTT 3 cut(s) 8, 752, 1586
HinfI GANTC 7 cut(s) 356, 406, 627, 704, 1106, 1417, 1541
HpaI GTTAAC 1 cut(s) 636
HpaII CCGG 3 cut(s) 21, 213, 218
HphI GGTGA 1 cut(s) 1247
Hpy166II GTNNAC 6 cut(s) 274, 487, 605, 636, 1378, 1509
Hpy188I TCNGA 7 cut(s) 53, 120, 196, 333, 371, 1416, 1540
Hpy188III TCNNGA 6 cut(s) 67, 244, 395, 439, 492, 968
Hpy8I GTNNAC 6 cut(s) 274, 487, 605, 636, 1378, 1509
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 7 cut(s) 224, 305, 521, 593, 690, 708, 982
HpyCH4III ACNGT 8 cut(s) 210, 365, 566, 613, 667, 1040, 1217, 1410
HpyCH4IV ACGT 3 cut(s) 225, 607, 919
HpyF10VI GCNNNNNNNGC 6 cut(s) 259, 546, 879, 1053, 1072, 1470
HpyF3I CTNAG 9 cut(s) 50, 193, 413, 490, 503, 956, 1264, 1413, 1566
HpySE526I ACGT 3 cut(s) 225, 607, 919
Hsp92II CATG 8 cut(s) 261, 515, 625, 644, 781, 815, 1141, 1196
Ksp22I TGATCA 1 cut(s) 1156
KspAI GTTAAC 1 cut(s) 636
LguI GCTCTTC 1 cut(s) 1454
LmnI GCTCC 3 cut(s) 115, 283, 548
Lsp1109I GCAGC 1 cut(s) 552
LweI GCATC 2 cut(s) 262, 1034
MaeI CTAG 2 cut(s) 107, 1611
MaeII ACGT 3 cut(s) 225, 607, 919
MaeIII GTNAC 2 cut(s) 1103, 1235
MfeI CAATTG 1 cut(s) 1386
MflI RGATCY 1 cut(s) 867
MhlI GDGCHC 2 cut(s) 464, 511
MlyI GAGTC 2 cut(s) 415, 1100
MseI TTAA 7 cut(s) 384, 635, 785, 795, 909, 1148, 1212
MslI CAYNNNNRTG 1 cut(s) 1197
MspI CCGG 3 cut(s) 21, 213, 218
MspR9I CCNGG 3 cut(s) 218, 1089, 1369
MunI CAATTG 1 cut(s) 1386
MvaI CCWGG 2 cut(s) 1089, 1369
MwoI GCNNNNNNNGC 6 cut(s) 259, 546, 879, 1053, 1072, 1470
NciI CCSGG 1 cut(s) 218
NcoI CCATGG 1 cut(s) 257
NdeI CATATG 1 cut(s) 577
NlaIII CATG 8 cut(s) 261, 515, 625, 644, 781, 815, 1141, 1196
NlaIV GGNNCC 1 cut(s) 461
NmeAIII GCCGAG 1 cut(s) 167
NmuCI GTSAC 2 cut(s) 1103, 1235
NspI RCATGY 3 cut(s) 515, 815, 1141
PaqCI CACCTGC 1 cut(s) 1293
PceI AGGCCT 1 cut(s) 1615
PciI ACATGT 1 cut(s) 511
PciSI GCTCTTC 1 cut(s) 1454
PfeI GAWTC 5 cut(s) 356, 627, 704, 1417, 1541
PflMI CCANNNNNTGG 1 cut(s) 1391
PfoI TCCNGGA 1 cut(s) 1087
PkrI GCNGC 2 cut(s) 501, 542
PleI GAGTC 2 cut(s) 414, 1100
PpsI GAGTC 2 cut(s) 414, 1100
PscI ACATGT 1 cut(s) 511
Psp6I CCWGG 2 cut(s) 1087, 1367
PspGI CCWGG 2 cut(s) 1087, 1367
PspN4I GGNNCC 1 cut(s) 461
PspPI GGNCC 6 cut(s) 215, 241, 274, 347, 586, 1167
PstNI CAGNNNCTG 1 cut(s) 546
PsuI RGATCY 1 cut(s) 867
RsaI GTAC 3 cut(s) 610, 740, 833
RsaNI GTAC 3 cut(s) 609, 739, 832
RseI CAYNNNNRTG 1 cut(s) 1197
SapI GCTCTTC 1 cut(s) 1454
SaqAI TTAA 7 cut(s) 384, 635, 785, 795, 909, 1148, 1212
SatI GCNGC 2 cut(s) 500, 541
Sau96I GGNCC 6 cut(s) 215, 241, 274, 347, 586, 1167
SchI GAGTC 2 cut(s) 415, 1100
ScrFI CCNGG 3 cut(s) 218, 1089, 1369
SduI GDGCHC 2 cut(s) 464, 511
SfaNI GCATC 2 cut(s) 262, 1034
SinI GGWCC 3 cut(s) 241, 274, 347
SmiMI CAYNNNNRTG 1 cut(s) 1197
SseBI AGGCCT 1 cut(s) 1615
SsiI CCGC 5 cut(s) 239, 262, 375, 499, 549
SspMI CTAG 2 cut(s) 107, 1611
StuI AGGCCT 1 cut(s) 1615
StyD4I CCNGG 3 cut(s) 216, 1087, 1367
StyI CCWWGG 1 cut(s) 257
TaaI ACNGT 8 cut(s) 210, 365, 566, 613, 667, 1040, 1217, 1410
TaiI ACGT 3 cut(s) 228, 610, 922
TaqI TCGA 6 cut(s) 126, 157, 359, 404, 425, 1255
TatI WGTACW 2 cut(s) 738, 831
TauI GCSGC 1 cut(s) 502
TfiI GAWTC 5 cut(s) 356, 627, 704, 1417, 1541
Tru1I TTAA 7 cut(s) 384, 635, 785, 795, 909, 1148, 1212
Tru9I TTAA 7 cut(s) 384, 635, 785, 795, 909, 1148, 1212
TscAI CASTG 5 cut(s) 672, 714, 751, 1058, 1246
TseFI GTSAC 2 cut(s) 1103, 1235
TseI GCWGC 1 cut(s) 540
Tsp45I GTSAC 2 cut(s) 1103, 1235
TspDTI ATGAA 9 cut(s) 156, 192, 540, 612, 866, 1209, 1512, 1533, 1569
TspGWI ACGGA 1 cut(s) 1562
TspRI CASTG 5 cut(s) 672, 714, 751, 1058, 1246
Van91I CCANNNNNTGG 1 cut(s) 1391
VpaK11BI GGWCC 3 cut(s) 241, 274, 347
XapI RAATTY 5 cut(s) 30, 62, 128, 179, 1024
XceI RCATGY 3 cut(s) 515, 815, 1141
XcmI CCANNNNNNNNNTGG 1 cut(s) 264
XmiI GTMKAC 2 cut(s) 1377, 1508
XspI CTAG 2 cut(s) 107, 1611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.