Rh5BG052400

May participate in a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001097
Physical Location & Seq
Reverse (-)
4133999 .. 4149700
15702 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG052400.1

Sequence Viewer

Length: 1257 bp
ATGATTTTTTTCTTTTTTGGGGTAAATATGCGGAGTCTCACTGGTCATACAAGTCCAGTTGAATCTGTAGCTTTTAATTCAGCAGAAGTTTTGGTGCTTGCTGGAGCTTCTACTGGCGCAACAAAAATAAGGGATCTGGAGGAAACAAAGATGGATCGTATACTTACCGGACACAGATCCAGCTGTTCTGCTGTTAAATTTCATCCATTTGGTGAGTTCTTTGCTTCTGGTTCCATGGACACTAATCTGAAGATCTGGGACATTAGAAAGAAGGGATGCATCCACACATACAAAGGTCATACTCAAGGCATTAGTACTATTAAGTTCACTCCCGATGGTCGATGGGTAGTTTCTGGTGGGTTTGACAATGTTCTAAAGGTGTGGGATCTAACTGCTGGGAAGCTCTTGCATGATTTTAAATTCCATGAACGACATATTCGGTCTATAGATTTTCATCCCCTTGAGTTTCTCTTGGCCACAGTTGTCGTTGTACCAGGAAGGGCTCGCACCCTGGTTGAGAGATTTGAAAGGAGAGAGAGATTTAGTAGCAATGACGATCAAGCAACTGGCGTATTTGGTCCCGTGATACCTGAATATCAATCAACCAGCATATCTCGTACTGTGATACCTGAAGATCAAGCAACTAGCATATGTTGTCCCGTGATGCATGAAATGGATAAAACATTCACCATGAAGCGAGAAGAAGAACCTCAAATTTCTGGGAGGCACATGTCATCTGCAAACAATGATCCTGCTGAAGATGTGATGCAAACTCATGATGCATTCCTAAGTACCCTTCGATCCCACCTGACAAAACTGCAGGTGGTGAGGCATTTTTGGGAACGAAATGATATTAAAGGTGCTATCAGTGCTATCAGAAAGCTGTCAGATCATTCGGTACAAGCAGATGTGATTAGTGTTCTCCTGGAGAAAATGGATATTCTGACATTAGATCTCTTTTCGTGTTTGCTGCCTGTGCTTCTGGGCTTACTTGATAGCAAAATTGAGAGGCATGCAAATGTGTCATTGGAGATGCTGTTGAAGCTAGTAGCAGTCTTTGGCCCAGTGATACGGTCAACCATTTCAGCACCTCCAGCGGTCGGAGTCAATCTTCAGGCAGAGCAAAGGCTGGAGTGCTGCAACCAATGCTCTGTACACCTGCAAAAGATCCAGAAAATTCTCCCAGTCTTTGTAAGGAAGGGGGGCGTGCTGGCAAGATGTGCTCTAGAATTGAATCTAATTCTTCAAGAGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0032991 GO:0044424 GO:0044464 GO:0080008 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Katanin_con80 WD40

Protein Analysis

418

Amino Acids

46.78

Weight (kDa)

7.68

Isoelectric Point (pI)

44.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 9 - 160 1.2e-29 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 10 - 132 6.6e-17 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 10 - 91 4.7e-09 G protein beta WD-40 repeat protein
WD40_Prp19 PF24814 11 - 160 1e-24 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 11 - 99 3.3e-13 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 14 - 160 1.7e-29 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 14 - 162 3.9e-19 WDR36/Utp21 second beta-propeller domain
Beta-prop_SPT8 PF23798 15 - 111 3.6e-07 SPT8 beta propeller
WD40_CDC20-Fz PF24807 15 - 155 1.7e-21 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 17 - 128 2.2e-08 WDR36/Utp21 first beta-propeller
WDR55 PF24796 18 - 153 4.2e-12 WDR55
Beta-prop_TEP1_2nd PF25047 23 - 149 1e-09 TEP-1 second beta-propeller
WD40_MABP1-WDR62_2nd PF24782 32 - 130 3.5e-08 MABP1/WDR62 second WD40 domain
Beta-prop_WDR90_POC16_2nd PF23393 36 - 120 4.7e-07 WDR90/POC16, second beta-propeller
WD40_MABP1-WDR62_1st PF24780 42 - 134 1.4e-08 MABP1/WDR62 first WD40 domain
Beta-prop_EML_2 PF23414 43 - 152 2.3e-11 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 53 - 87 5.7e-09 WD domain, G-beta repeat
Beta-prop_EML PF23409 54 - 155 3.4e-08 Echinoderm microtubule-associated protein first beta-propeller
EIF3I PF24805 54 - 161 4.2e-12 EIF3I
Beta-prop_WDR5 PF25175 55 - 160 7.6e-26 WDR5 beta-propeller domain
Beta-prop_WDR75_1st PF23869 57 - 131 6.6e-06 WD repeat-containing protein 75 first beta-propeller
Beta-prop_Aladin PF25460 59 - 132 2.3e-08 Aladin seven-bladed propeller
WD40_Gbeta PF25391 79 - 158 1.7e-08 G protein beta WD-40 repeat protein
WD40 PF00400 93 - 129 8.5e-12 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 95 - 161 2.6e-06 CAF1B/HIR1 beta-propeller domain
Katanin_con80 PF13925 257 - 414 7.9e-52 con80 domain of Katanin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 811, 1167
Acc36I ACCTGC 2 cut(s) 811, 1167
AccI GTMKAC 1 cut(s) 160
AciI CCGC 2 cut(s) 31, 1097
AclWI GGATC 7 cut(s) 141, 162, 171, 393, 743, 795, 1162
AcoI YGGCCR 1 cut(s) 474
AcsI RAATTY 4 cut(s) 197, 419, 714, 1176
AcuI CTGAAG 4 cut(s) 269, 651, 777, 1097
AfaI GTAC 6 cut(s) 316, 492, 619, 793, 900, 1155
AfiI CCNNNNNNNGG 1 cut(s) 1100
AflIII ACRYGT 1 cut(s) 729
AgsI TTSAA 5 cut(s) 62, 527, 1042, 1234, 1247
AjnI CCWGG 3 cut(s) 493, 510, 924
AluBI AGCT 6 cut(s) 71, 107, 183, 403, 883, 1045
AluI AGCT 6 cut(s) 71, 107, 183, 403, 883, 1045
Alw21I GWGCWC 1 cut(s) 1225
Alw26I GTCTC 1 cut(s) 41
AlwI GGATC 7 cut(s) 141, 162, 171, 393, 743, 795, 1162
AoxI GGCC 2 cut(s) 474, 1060
ApeKI GCWGC 2 cut(s) 970, 1137
ApoI RAATTY 4 cut(s) 197, 419, 714, 1176
AspLEI GCGC 1 cut(s) 119
AspS9I GGNCC 2 cut(s) 578, 1061
AsuHPI GGTGA 3 cut(s) 224, 679, 838
AvaII GGWCC 1 cut(s) 578
BalI TGGCCA 1 cut(s) 476
BanII GRGCYC 1 cut(s) 505
Bbv12I GWGCWC 1 cut(s) 1225
BbvI GCAGC 2 cut(s) 957, 1124
BccI CCATC 3 cut(s) 145, 329, 336
BciT130I CCWGG 3 cut(s) 495, 512, 926
BcoDI GTCTC 1 cut(s) 41
BfaI CTAG 3 cut(s) 645, 1046, 1226
BfmI CTRYAG 3 cut(s) 66, 444, 818
BfuAI ACCTGC 2 cut(s) 811, 1167
BglII AGATCT 2 cut(s) 252, 952
BisI GCNGC 2 cut(s) 971, 1138
BlsI GCNGC 2 cut(s) 972, 1139
BmcAI AGTACT 1 cut(s) 316
Bme1390I CCNGG 3 cut(s) 495, 512, 926
Bme18I GGWCC 1 cut(s) 578
BmgT120I GGNCC 2 cut(s) 578, 1061
BmiI GGNNCC 2 cut(s) 232, 580
BmrFI CCNGG 3 cut(s) 495, 512, 926
BmrI ACTGGG 2 cut(s) 1058, 1178
BmsI GCATC 6 cut(s) 266, 288, 654, 756, 769, 1023
BmuI ACTGGG 2 cut(s) 1058, 1178
BpmI CTGGAG 5 cut(s) 123, 158, 947, 1077, 1151
BpuEI CTTGAG 2 cut(s) 288, 482
BsaBI GATNNNNATC 1 cut(s) 453
BsaJI CCNNGG 2 cut(s) 234, 510
BsaWI WCCGGW 1 cut(s) 167
BsaXI ACNNNNNCTCC 2 cut(s) 1022, 1052
Bsc4I CCNNNNNNNGG 1 cut(s) 1100
Bse1I ACTGG 6 cut(s) 46, 56, 118, 571, 1064, 1184
Bse3DI GCAATG 1 cut(s) 556
Bse8I GATNNNNATC 1 cut(s) 453
BseBI CCWGG 3 cut(s) 495, 512, 926
BseDI CCNNGG 2 cut(s) 234, 510
BseGI GGATG 4 cut(s) 202, 279, 281, 454
BseJI GATNNNNATC 1 cut(s) 453
BseLI CCNNNNNNNGG 1 cut(s) 1100
BseMI GCAATG 1 cut(s) 556
BseNI ACTGG 6 cut(s) 46, 56, 118, 571, 1064, 1184
BseXI GCAGC 2 cut(s) 957, 1124
BseYI CCCAGC 1 cut(s) 395
Bsh1285I CGRYCG 1 cut(s) 1101
BshFI GGCC 2 cut(s) 476, 1062
BsiEI CGRYCG 1 cut(s) 1101
BsiHKAI GWGCWC 1 cut(s) 1225
BsiSI CCGG 1 cut(s) 168
BslFI GGGAC 3 cut(s) 272, 564, 642
BslI CCNNNNNNNGG 1 cut(s) 1100
BsmAI GTCTC 1 cut(s) 41
BsmFI GGGAC 3 cut(s) 272, 564, 642
BsmI GAATGC 1 cut(s) 782
BsnI GGCC 2 cut(s) 476, 1062
Bsp1286I GDGCHC 2 cut(s) 505, 1225
Bsp1407I TGTACA 1 cut(s) 1153
Bsp19I CCATGG 1 cut(s) 234
BspACI CCGC 2 cut(s) 31, 1097
BspANI GGCC 2 cut(s) 476, 1062
BspHI TCATGA 1 cut(s) 775
BspLI GGNNCC 2 cut(s) 232, 580
BspMAI CTGCAG 1 cut(s) 822
BspMI ACCTGC 2 cut(s) 811, 1167
BspPI GGATC 7 cut(s) 141, 162, 171, 393, 743, 795, 1162
BsrDI GCAATG 1 cut(s) 556
BsrGI TGTACA 1 cut(s) 1153
BsrI ACTGG 6 cut(s) 46, 56, 118, 571, 1064, 1184
BssECI CCNNGG 2 cut(s) 234, 510
BssNAI GTATAC 1 cut(s) 161
BssT1I CCWWGG 1 cut(s) 234
Bst1107I GTATAC 1 cut(s) 161
Bst2UI CCWGG 3 cut(s) 495, 512, 926
Bst4CI ACNGT 3 cut(s) 481, 622, 1074
BstAPI GCANNNNNTGC 2 cut(s) 1146, 1220
BstAUI TGTACA 1 cut(s) 1153
BstC8I GCNNGC 5 cut(s) 99, 505, 1014, 1208, 1212
BstDEI CTNAG 1 cut(s) 788
BstDSI CCRYGG 1 cut(s) 234
BstF5I GGATG 4 cut(s) 202, 279, 281, 454
BstHHI GCGC 1 cut(s) 119
BstMAI GTCTC 1 cut(s) 41
BstMCI CGRYCG 1 cut(s) 1101
BstMWI GCNNNNNNNGC 6 cut(s) 869, 976, 1042, 1094, 1146, 1220
BstNI CCWGG 3 cut(s) 495, 512, 926
BstNSI RCATGY 2 cut(s) 733, 1016
BstSCI CCNGG 3 cut(s) 493, 510, 924
BstSFI CTRYAG 3 cut(s) 66, 444, 818
BstV1I GCAGC 2 cut(s) 957, 1124
BstX2I RGATCY 6 cut(s) 133, 176, 252, 385, 952, 1167
BstYI RGATCY 6 cut(s) 133, 176, 252, 385, 952, 1167
BstZ17I GTATAC 1 cut(s) 161
BsuRI GGCC 2 cut(s) 476, 1062
BtgI CCRYGG 1 cut(s) 234
BtsCI GGATG 4 cut(s) 202, 279, 281, 454
BtsIMutI CAGTG 3 cut(s) 39, 874, 1071
BveI ACCTGC 2 cut(s) 811, 1167
Cac8I GCNNGC 5 cut(s) 99, 505, 1014, 1208, 1212
CciI TCATGA 1 cut(s) 775
CfoI GCGC 1 cut(s) 119
Cfr13I GGNCC 2 cut(s) 578, 1061
Csp6I GTAC 6 cut(s) 315, 491, 618, 792, 899, 1154
CviAII CATG 8 cut(s) 235, 410, 425, 668, 691, 730, 776, 1013
CviQI GTAC 6 cut(s) 315, 491, 618, 792, 899, 1154
DdeI CTNAG 1 cut(s) 788
DraI TTTAAA 1 cut(s) 418
EaeI YGGCCR 1 cut(s) 474
Eco130I CCWWGG 1 cut(s) 234
Eco24I GRGCYC 1 cut(s) 505
Eco47I GGWCC 1 cut(s) 578
Eco57I CTGAAG 4 cut(s) 269, 651, 777, 1097
EcoRII CCWGG 3 cut(s) 493, 510, 924
EcoT14I CCWWGG 1 cut(s) 234
EcoT22I ATGCAT 3 cut(s) 281, 669, 784
EcoT38I GRGCYC 1 cut(s) 505
ErhI CCWWGG 1 cut(s) 234
FaeI CATG 8 cut(s) 238, 413, 428, 671, 694, 733, 779, 1016
FaqI GGGAC 3 cut(s) 272, 564, 642
FatI CATG 8 cut(s) 234, 409, 424, 667, 690, 729, 775, 1012
FauNDI CATATG 1 cut(s) 650
FblI GTMKAC 1 cut(s) 160
Fnu4HI GCNGC 2 cut(s) 971, 1138
FokI GGATG 4 cut(s) 189, 266, 288, 441
FriOI GRGCYC 1 cut(s) 505
Fsp4HI GCNGC 2 cut(s) 971, 1138
FspBI CTAG 3 cut(s) 645, 1046, 1226
GlaI GCGC 1 cut(s) 118
GluI GCNGC 2 cut(s) 971, 1138
GsaI CCCAGC 1 cut(s) 399
GsuI CTGGAG 5 cut(s) 123, 158, 947, 1077, 1151
HaeIII GGCC 2 cut(s) 476, 1062
HapII CCGG 1 cut(s) 168
HhaI GCGC 1 cut(s) 119
Hin1II CATG 8 cut(s) 238, 413, 428, 671, 694, 733, 779, 1016
Hin6I GCGC 1 cut(s) 117
HinP1I GCGC 1 cut(s) 117
HincII GTYRAC 1 cut(s) 1077
HindII GTYRAC 1 cut(s) 1077
HinfI GANTC 5 cut(s) 34, 62, 1104, 1234, 1250
HpaII CCGG 1 cut(s) 168
HphI GGTGA 3 cut(s) 224, 679, 838
Hpy166II GTNNAC 4 cut(s) 161, 327, 1077, 1156
Hpy188I TCNGA 5 cut(s) 249, 878, 889, 945, 1103
Hpy188III TCNNGA 6 cut(s) 137, 332, 776, 1171, 1226, 1247
Hpy8I GTNNAC 4 cut(s) 161, 327, 1077, 1156
HpyAV CCTTC 4 cut(s) 265, 492, 806, 1192
HpyCH4III ACNGT 3 cut(s) 481, 622, 1074
HpyF10VI GCNNNNNNNGC 6 cut(s) 869, 976, 1042, 1094, 1146, 1220
HpyF3I CTNAG 1 cut(s) 788
Hsp92II CATG 8 cut(s) 238, 413, 428, 671, 694, 733, 779, 1016
HspAI GCGC 1 cut(s) 117
LmnI GCTCC 1 cut(s) 104
Lsp1109I GCAGC 2 cut(s) 957, 1124
LweI GCATC 6 cut(s) 266, 288, 654, 756, 769, 1023
MaeI CTAG 3 cut(s) 645, 1046, 1226
MboII GAAGA 7 cut(s) 262, 644, 713, 716, 770, 1103, 1235
MflI RGATCY 6 cut(s) 133, 176, 252, 385, 952, 1167
MhlI GDGCHC 2 cut(s) 505, 1225
MlsI TGGCCA 1 cut(s) 476
MluCI AATT 8 cut(s) 76, 197, 419, 714, 1002, 1176, 1229, 1239
MluNI TGGCCA 1 cut(s) 476
MlyI GAGTC 2 cut(s) 43, 1113
MmeI TCCRAC 1 cut(s) 1081
MnlI CCTC 6 cut(s) 133, 717, 720, 822, 1002, 1101
Mox20I TGGCCA 1 cut(s) 476
Mph1103I ATGCAT 3 cut(s) 281, 669, 784
MscI TGGCCA 1 cut(s) 476
MseI TTAA 5 cut(s) 75, 195, 321, 417, 855
MslI CAYNNNNRTG 1 cut(s) 1017
Msp20I TGGCCA 1 cut(s) 476
MspA1I CMGCKG 2 cut(s) 183, 1097
MspI CCGG 1 cut(s) 168
MspR9I CCNGG 3 cut(s) 495, 512, 926
Mva1269I GAATGC 1 cut(s) 782
MvaI CCWGG 3 cut(s) 495, 512, 926
MwoI GCNNNNNNNGC 6 cut(s) 869, 976, 1042, 1094, 1146, 1220
NcoI CCATGG 1 cut(s) 234
NdeI CATATG 1 cut(s) 650
NlaIII CATG 8 cut(s) 238, 413, 428, 671, 694, 733, 779, 1016
NlaIV GGNNCC 2 cut(s) 232, 580
NsiI ATGCAT 3 cut(s) 281, 669, 784
NspI RCATGY 2 cut(s) 733, 1016
PaeI GCATGC 1 cut(s) 1016
PagI TCATGA 1 cut(s) 775
PaqCI CACCTGC 2 cut(s) 811, 1167
PciI ACATGT 1 cut(s) 729
PctI GAATGC 1 cut(s) 782
PfeI GAWTC 2 cut(s) 62, 1234
PfoI TCCNGGA 1 cut(s) 924
PkrI GCNGC 2 cut(s) 972, 1139
PleI GAGTC 2 cut(s) 42, 1112
PpsI GAGTC 2 cut(s) 42, 1112
PscI ACATGT 1 cut(s) 729
Psp6I CCWGG 3 cut(s) 493, 510, 924
PspFI CCCAGC 1 cut(s) 395
PspGI CCWGG 3 cut(s) 493, 510, 924
PspN4I GGNNCC 2 cut(s) 232, 580
PspPI GGNCC 2 cut(s) 578, 1061
PstI CTGCAG 1 cut(s) 822
PsuI RGATCY 6 cut(s) 133, 176, 252, 385, 952, 1167
PvuII CAGCTG 1 cut(s) 183
RsaI GTAC 6 cut(s) 316, 492, 619, 793, 900, 1155
RsaNI GTAC 6 cut(s) 315, 491, 618, 792, 899, 1154
RseI CAYNNNNRTG 1 cut(s) 1017
SaqAI TTAA 5 cut(s) 75, 195, 321, 417, 855
SatI GCNGC 2 cut(s) 971, 1138
Sau96I GGNCC 2 cut(s) 578, 1061
ScaI AGTACT 1 cut(s) 316
SchI GAGTC 2 cut(s) 43, 1113
ScrFI CCNGG 3 cut(s) 495, 512, 926
SduI GDGCHC 2 cut(s) 505, 1225
SfaNI GCATC 6 cut(s) 266, 288, 654, 756, 769, 1023
SfcI CTRYAG 3 cut(s) 66, 444, 818
SinI GGWCC 1 cut(s) 578
SmiMI CAYNNNNRTG 1 cut(s) 1017
SmlI CTYRAG 2 cut(s) 303, 461
SmoI CTYRAG 2 cut(s) 303, 461
SphI GCATGC 1 cut(s) 1016
Sse9I AATT 8 cut(s) 76, 197, 419, 714, 1002, 1176, 1229, 1239
SsiI CCGC 2 cut(s) 31, 1097
SspMI CTAG 3 cut(s) 645, 1046, 1226
StyD4I CCNGG 3 cut(s) 493, 510, 924
StyI CCWWGG 1 cut(s) 234
TaaI ACNGT 3 cut(s) 481, 622, 1074
TaqI TCGA 2 cut(s) 340, 799
TaqII GACCGA 1 cut(s) 429
TasI AATT 8 cut(s) 76, 197, 419, 714, 1002, 1176, 1229, 1239
TatI WGTACW 2 cut(s) 314, 1153
TfiI GAWTC 2 cut(s) 62, 1234
Tru1I TTAA 5 cut(s) 75, 195, 321, 417, 855
Tru9I TTAA 5 cut(s) 75, 195, 321, 417, 855
TscAI CASTG 3 cut(s) 46, 874, 1071
TseI GCWGC 2 cut(s) 970, 1137
TspDTI ATGAA 5 cut(s) 191, 441, 443, 684, 707
TspRI CASTG 3 cut(s) 46, 874, 1071
VpaK11BI GGWCC 1 cut(s) 578
XapI RAATTY 4 cut(s) 197, 419, 714, 1176
XbaI TCTAGA 1 cut(s) 1225
XceI RCATGY 2 cut(s) 733, 1016
XmiI GTMKAC 1 cut(s) 160
XspI CTAG 3 cut(s) 645, 1046, 1226
ZrmI AGTACT 1 cut(s) 316
Zsp2I ATGCAT 3 cut(s) 281, 669, 784
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.