AT2G28680

Cupin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001439
Physical Location & Seq
Reverse (-)
12302959 .. 12304830
1872 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G28680.1

Sequence Viewer

Length: 1071 bp
ATGGAATTGGATCTATCACCAAGGCTCCCGAAAAAGGTGTACGGAGGAGACGGTGGTTCGTACTTTGCATGGTGCCCTGAAGAGTTGCCAATGCTCCGAGATGGTAACATTGGAGCCTCTAAGCTCGCTCTTGAGAAGTATGGCTTGGCTCTTCCTCGCTACTCTGACTCCCCCAAGGTCGCTTATGTTCTTCAAGGAGCTGGAACAGCTGGAATCGTGCTCCCTGAGAAGGAGGAGAAAGTGATAGCAATCAAGAAAGGAGACTCCATAGCTTTACCTTTTGGTGTAGTGACATGGTGGTTCAACAATGAGGACACTGAGCTAGTCGTTCTCTTCCTTGGTGAGACTCACAAGGGTCACAAAGCTGGACAGTTCACCGACTTTTACCTAACCGGATCCAATGGAATCTTCACCGGTTTCTCTACTGAGTTTGTTGGCAGAGCTTGGGATCTCGATGAGACCACCGTGAAGAAGCTTGTCGGATCTCAGACAGGTAACGGTATCGTGAAGGTTGATGCGAGCTTGAAGATGCCTGAACCAAAGAAAGGTGACCGAAAAGGGTTTGTTTTGAACTGTTTGGAGGCTCCTCTTGATGTTGACATTAAGGATGGAGGAAGAGTTGTTGTGTTGAACACAAAGAATCTTCCTTTGGTTGGTGAAGTTGGATTTGGAGCTGATCTTGTGAGAATCGATGGACACTCTATGTGTTCTCCTGGATTCTCTTGTGACTCGGCTCTTCAAGTTACTTACATTGTTGGTGGAAGTGGTCGTGTTCAGATTGTTGGTGCTGATGGGAAGAGAGTTCTTGAGACTCATGTGAAAGCTGGTGTTTTGTTCATTGTTCCTAGGTTCTTTGTTGTCTCCAAGATTGCTGATTCTGATGGCTTGTCTTGGTTCTCCATTGTGACCACTCCTGATCCAATATTCACTCATTTGGCCGGGAGGACTTCGGTGTGGAAGGCTTTGTCGCCGGAGGTTTTGCAGGCAGCGTTCAAGGTGGATCCCGAGGTTGAGAAGGCTTTCCGATCTAAGAGGACCTCTGATGCCATTTTCTTCTCTCCTTCCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

356

Amino Acids

38.46

Weight (kDa)

6.26

Isoelectric Point (pI)

28.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 10 - 157 2.5e-25 Cupin
Cupin_1 PF00190 196 - 338 4.4e-25 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 72
AclWI GGATC 8 cut(s) 18, 390, 403, 456, 490, 911, 995, 1008
AcoI YGGCCR 1 cut(s) 936
AcuI CTGAAG 1 cut(s) 99
AfaI GTAC 2 cut(s) 41, 62
AfiI CCNNNNNNNGG 4 cut(s) 34, 229, 545, 653
AgeI ACCGGT 1 cut(s) 413
AgsI TTSAA 7 cut(s) 194, 304, 526, 571, 631, 740, 994
AjnI CCWGG 1 cut(s) 712
AjuI GAANNNNNNNTTGG 8 cut(s) 128, 160, 392, 424, 632, 651, 664, 683
Alw21I GWGCWC 1 cut(s) 222
Alw26I GTCTC 6 cut(s) 42, 255, 338, 452, 803, 865
AlwI GGATC 8 cut(s) 18, 390, 403, 456, 490, 911, 995, 1008
Ama87I CYCGRG 1 cut(s) 1004
AoxI GGCC 1 cut(s) 936
ApeKI GCWGC 1 cut(s) 986
AsiGI ACCGGT 1 cut(s) 413
Asp700I GAANNNNTTC 1 cut(s) 1019
AspA2I CCTAGG 1 cut(s) 845
AspS9I GGNCC 1 cut(s) 1035
AsuC2I CCSGG 1 cut(s) 940
AsuHPI GGTGA 6 cut(s) 9, 353, 367, 403, 560, 668
AvaI CYCGRG 1 cut(s) 1004
AvaII GGWCC 1 cut(s) 1035
AvrII CCTAGG 1 cut(s) 845
BaeGI GKGCMC 1 cut(s) 77
BamHI GGATCC 2 cut(s) 395, 1000
BanI GGYRCC 1 cut(s) 72
Bbv12I GWGCWC 1 cut(s) 222
BbvI GCAGC 1 cut(s) 998
BccI CCATC 5 cut(s) 95, 602, 686, 785, 875
BciT130I CCWGG 1 cut(s) 714
BcnI CCSGG 1 cut(s) 940
BcoDI GTCTC 6 cut(s) 42, 255, 338, 452, 803, 865
BfaI CTAG 3 cut(s) 323, 846, 1069
BisI GCNGC 1 cut(s) 987
BlnI CCTAGG 1 cut(s) 845
BlsI GCNGC 1 cut(s) 988
Bme1390I CCNGG 2 cut(s) 714, 940
Bme18I GGWCC 1 cut(s) 1035
BmeT110I CYCGRG 1 cut(s) 1004
BmgT120I GGNCC 1 cut(s) 1035
BmiI GGNNCC 6 cut(s) 26, 74, 115, 397, 585, 1002
BmrFI CCNGG 2 cut(s) 714, 940
BmsI GCATC 3 cut(s) 505, 519, 1033
BpuEI CTTGAG 2 cut(s) 152, 827
BpuMI CCSGG 1 cut(s) 940
Bsa29I ATCGAT 1 cut(s) 690
BsaBI GATNNNNATC 1 cut(s) 248
BsaI GGTCTC 1 cut(s) 452
BsaJI CCNNGG 5 cut(s) 20, 174, 337, 845, 1005
BsaWI WCCGGW 2 cut(s) 392, 413
BsaXI ACNNNNNCTCC 6 cut(s) 9, 39, 152, 182, 603, 633
Bsc4I CCNNNNNNNGG 4 cut(s) 34, 229, 545, 653
Bse118I RCCGGY 1 cut(s) 413
Bse8I GATNNNNATC 1 cut(s) 248
BseBI CCWGG 1 cut(s) 714
BseCI ATCGAT 1 cut(s) 690
BseDI CCNNGG 5 cut(s) 20, 174, 337, 845, 1005
BseGI GGATG 1 cut(s) 613
BseJI GATNNNNATC 1 cut(s) 248
BseLI CCNNNNNNNGG 4 cut(s) 34, 229, 545, 653
BseMII CTCAG 4 cut(s) 216, 309, 417, 500
BseRI GAGGAG 3 cut(s) 60, 248, 576
BseSI GKGCMC 1 cut(s) 77
BseXI GCAGC 1 cut(s) 998
BshFI GGCC 1 cut(s) 938
BshNI GGYRCC 1 cut(s) 72
BshTI ACCGGT 1 cut(s) 413
BshVI ATCGAT 1 cut(s) 690
BsiHKAI GWGCWC 1 cut(s) 222
BsiHKCI CYCGRG 1 cut(s) 1004
BsiSI CCGG 4 cut(s) 393, 414, 939, 971
BslI CCNNNNNNNGG 4 cut(s) 34, 229, 545, 653
BsmAI GTCTC 6 cut(s) 42, 255, 338, 452, 803, 865
BsmBI CGTCTC 1 cut(s) 42
BsnI GGCC 1 cut(s) 938
Bso31I GGTCTC 1 cut(s) 452
BsoBI CYCGRG 1 cut(s) 1004
Bsp1286I GDGCHC 2 cut(s) 77, 222
Bsp143I GATC 8 cut(s) 10, 395, 448, 482, 676, 916, 1000, 1025
BspANI GGCC 1 cut(s) 938
BspCNI CTCAG 4 cut(s) 217, 310, 418, 499
BspDI ATCGAT 1 cut(s) 690
BspLI GGNNCC 6 cut(s) 26, 74, 115, 397, 585, 1002
BspPI GGATC 8 cut(s) 18, 390, 403, 456, 490, 911, 995, 1008
BspQI GCTCTTC 2 cut(s) 156, 741
BspT107I GGYRCC 1 cut(s) 72
BspTNI GGTCTC 1 cut(s) 452
BsrFI RCCGGY 1 cut(s) 413
BssAI RCCGGY 1 cut(s) 413
BssECI CCNNGG 5 cut(s) 20, 174, 337, 845, 1005
BssMI GATC 8 cut(s) 10, 395, 448, 482, 676, 916, 1000, 1025
BssT1I CCWWGG 4 cut(s) 20, 174, 337, 845
Bst2UI CCWGG 1 cut(s) 714
Bst4CI ACNGT 5 cut(s) 53, 372, 466, 500, 575
Bst6I CTCTTC 6 cut(s) 75, 156, 338, 610, 741, 791
BstC8I GCNNGC 3 cut(s) 126, 520, 984
BstDEI CTNAG 6 cut(s) 120, 225, 318, 426, 486, 1029
BstEII GGTNACC 1 cut(s) 548
BstF5I GGATG 1 cut(s) 613
BstKTI GATC 8 cut(s) 13, 398, 451, 485, 679, 919, 1003, 1028
BstMAI GTCTC 6 cut(s) 42, 255, 338, 452, 803, 865
BstMBI GATC 8 cut(s) 10, 395, 448, 482, 676, 916, 1000, 1025
BstMWI GCNNNNNNNGC 1 cut(s) 206
BstNI CCWGG 1 cut(s) 714
BstPI GGTNACC 1 cut(s) 548
BstSCI CCNGG 2 cut(s) 712, 938
BstSLI GKGCMC 1 cut(s) 77
BstV1I GCAGC 1 cut(s) 998
BstX2I RGATCY 5 cut(s) 10, 395, 448, 482, 1000
BstYI RGATCY 5 cut(s) 10, 395, 448, 482, 1000
Bsu15I ATCGAT 1 cut(s) 690
BsuRI GGCC 1 cut(s) 938
BsuTUI ATCGAT 1 cut(s) 690
BtsCI GGATG 1 cut(s) 613
BtsIMutI CAGTG 1 cut(s) 315
Cac8I GCNNGC 3 cut(s) 126, 520, 984
Cfr10I RCCGGY 1 cut(s) 413
Cfr13I GGNCC 1 cut(s) 1035
ClaI ATCGAT 1 cut(s) 690
Csp6I GTAC 2 cut(s) 40, 61
CspAI ACCGGT 1 cut(s) 413
CviAII CATG 3 cut(s) 69, 294, 815
CviQI GTAC 2 cut(s) 40, 61
DdeI CTNAG 6 cut(s) 120, 225, 318, 426, 486, 1029
DpnI GATC 8 cut(s) 12, 397, 450, 484, 678, 918, 1002, 1027
DpnII GATC 8 cut(s) 10, 395, 448, 482, 676, 916, 1000, 1025
EaeI YGGCCR 1 cut(s) 936
Eam1104I CTCTTC 6 cut(s) 75, 156, 338, 610, 741, 791
EarI CTCTTC 6 cut(s) 75, 156, 338, 610, 741, 791
Eco130I CCWWGG 4 cut(s) 20, 174, 337, 845
Eco31I GGTCTC 1 cut(s) 452
Eco47I GGWCC 1 cut(s) 1035
Eco57I CTGAAG 1 cut(s) 99
Eco88I CYCGRG 1 cut(s) 1004
Eco91I GGTNACC 1 cut(s) 548
EcoO109I RGGNCCY 1 cut(s) 1035
EcoO65I GGTNACC 1 cut(s) 548
EcoRII CCWGG 1 cut(s) 712
EcoT14I CCWWGG 4 cut(s) 20, 174, 337, 845
ErhI CCWWGG 4 cut(s) 20, 174, 337, 845
Esp3I CGTCTC 1 cut(s) 42
FaeI CATG 3 cut(s) 72, 297, 818
FaiI YATR 7 cut(s) 70, 141, 186, 269, 295, 704, 816
FalI AAGNNNNNCTT 2 cut(s) 128, 160
FatI CATG 3 cut(s) 68, 293, 814
Fnu4HI GCNGC 1 cut(s) 987
FokI GGATG 1 cut(s) 620
Fsp4HI GCNGC 1 cut(s) 987
FspBI CTAG 3 cut(s) 323, 846, 1069
GluI GCNGC 1 cut(s) 987
HaeIII GGCC 1 cut(s) 938
HapII CCGG 4 cut(s) 393, 414, 939, 971
Hin1II CATG 3 cut(s) 72, 297, 818
HincII GTYRAC 1 cut(s) 598
HindII GTYRAC 1 cut(s) 598
HindIII AAGCTT 1 cut(s) 473
HpaII CCGG 4 cut(s) 393, 414, 939, 971
HphI GGTGA 6 cut(s) 9, 353, 367, 403, 560, 668
Hpy166II GTNNAC 3 cut(s) 40, 375, 598
Hpy188I TCNGA 8 cut(s) 98, 166, 482, 489, 777, 880, 1025, 1042
Hpy188III TCNNGA 9 cut(s) 28, 131, 253, 452, 505, 590, 806, 914, 1004
Hpy8I GTNNAC 3 cut(s) 40, 375, 598
HpyAV CCTTC 5 cut(s) 223, 502, 952, 1009, 1071
HpyCH4III ACNGT 5 cut(s) 53, 372, 466, 500, 575
HpyCH4V TGCA 2 cut(s) 68, 982
HpyF10VI GCNNNNNNNGC 1 cut(s) 206
HpyF3I CTNAG 6 cut(s) 120, 225, 318, 426, 486, 1029
Hsp92II CATG 3 cut(s) 72, 297, 818
Kzo9I GATC 8 cut(s) 10, 395, 448, 482, 676, 916, 1000, 1025
LguI GCTCTTC 2 cut(s) 156, 741
LmnI GCTCC 7 cut(s) 30, 99, 113, 197, 225, 589, 671
Lsp1109I GCAGC 1 cut(s) 998
LweI GCATC 3 cut(s) 505, 519, 1033
MaeI CTAG 3 cut(s) 323, 846, 1069
MaeIII GTNAC 8 cut(s) 104, 289, 356, 494, 548, 725, 742, 904
MalI GATC 8 cut(s) 12, 397, 450, 484, 678, 918, 1002, 1027
MboI GATC 8 cut(s) 10, 395, 448, 482, 676, 916, 1000, 1025
MflI RGATCY 5 cut(s) 10, 395, 448, 482, 1000
MhlI GDGCHC 2 cut(s) 77, 222
MluCI AATT 1 cut(s) 5
MlyI GAGTC 5 cut(s) 161, 257, 340, 722, 805
MmeI TCCRAC 2 cut(s) 460, 643
MroXI GAANNNNTTC 1 cut(s) 1019
MseI TTAA 1 cut(s) 603
MspA1I CMGCKG 1 cut(s) 209
MspI CCGG 4 cut(s) 393, 414, 939, 971
MspR9I CCNGG 2 cut(s) 714, 940
MvaI CCWGG 1 cut(s) 714
MwoI GCNNNNNNNGC 1 cut(s) 206
NciI CCSGG 1 cut(s) 940
NdeII GATC 8 cut(s) 10, 395, 448, 482, 676, 916, 1000, 1025
NlaIII CATG 3 cut(s) 72, 297, 818
NlaIV GGNNCC 6 cut(s) 26, 74, 115, 397, 585, 1002
NmeAIII GCCGAG 1 cut(s) 710
NmuCI GTSAC 5 cut(s) 289, 356, 548, 725, 904
PciSI GCTCTTC 2 cut(s) 156, 741
PdmI GAANNNNTTC 1 cut(s) 1019
PfeI GAWTC 6 cut(s) 213, 405, 640, 687, 717, 875
PfoI TCCNGGA 1 cut(s) 712
PinAI ACCGGT 1 cut(s) 413
PkrI GCNGC 1 cut(s) 988
PleI GAGTC 5 cut(s) 161, 257, 340, 722, 805
PpsI GAGTC 5 cut(s) 161, 257, 340, 722, 805
PpuMI RGGWCCY 1 cut(s) 1035
Psp5II RGGWCCY 1 cut(s) 1035
Psp6I CCWGG 1 cut(s) 712
PspEI GGTNACC 1 cut(s) 548
PspGI CCWGG 1 cut(s) 712
PspN4I GGNNCC 6 cut(s) 26, 74, 115, 397, 585, 1002
PspPI GGNCC 1 cut(s) 1035
PspPPI RGGWCCY 1 cut(s) 1035
PsuI RGATCY 5 cut(s) 10, 395, 448, 482, 1000
PvuII CAGCTG 1 cut(s) 209
RsaI GTAC 2 cut(s) 41, 62
RsaNI GTAC 2 cut(s) 40, 61
SapI GCTCTTC 2 cut(s) 156, 741
SaqAI TTAA 1 cut(s) 603
SatI GCNGC 1 cut(s) 987
Sau3AI GATC 8 cut(s) 10, 395, 448, 482, 676, 916, 1000, 1025
Sau96I GGNCC 1 cut(s) 1035
SchI GAGTC 5 cut(s) 161, 257, 340, 722, 805
ScrFI CCNGG 2 cut(s) 714, 940
SduI GDGCHC 2 cut(s) 77, 222
SfaNI GCATC 3 cut(s) 505, 519, 1033
SinI GGWCC 1 cut(s) 1035
SmlI CTYRAG 2 cut(s) 131, 806
SmoI CTYRAG 2 cut(s) 131, 806
Sse9I AATT 1 cut(s) 5
SspI AATATT 1 cut(s) 924
SspMI CTAG 3 cut(s) 323, 846, 1069
StyD4I CCNGG 2 cut(s) 712, 938
StyI CCWWGG 4 cut(s) 20, 174, 337, 845
TaaI ACNGT 5 cut(s) 53, 372, 466, 500, 575
TaqI TCGA 2 cut(s) 453, 690
TaqII GACCGA 1 cut(s) 567
TasI AATT 1 cut(s) 5
TfiI GAWTC 6 cut(s) 213, 405, 640, 687, 717, 875
Tru1I TTAA 1 cut(s) 603
Tru9I TTAA 1 cut(s) 603
TscAI CASTG 1 cut(s) 322
TseFI GTSAC 5 cut(s) 289, 356, 548, 725, 904
TseI GCWGC 1 cut(s) 986
Tsp45I GTSAC 5 cut(s) 289, 356, 548, 725, 904
TspDTI ATGAA 1 cut(s) 826
TspGWI ACGGA 1 cut(s) 57
TspRI CASTG 1 cut(s) 322
VpaK11BI GGWCC 1 cut(s) 1035
XmaJI CCTAGG 1 cut(s) 845
XmnI GAANNNNTTC 1 cut(s) 1019
XspI CTAG 3 cut(s) 323, 846, 1069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.