Rorug06G0329800

Glutelin type-A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001439
Physical Location & Seq
Reverse (-)
48304522 .. 48307975
3454 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0329800.1

Sequence Viewer

Length: 1302 bp
ATGACCCACTTGGAGTTCTTCAAGAAAATCAAGCGCTTCAATCCTCTAGAGCCTTCTCTGGGCATTCTTGGCTTCTTCTTGGTCACTGTTTTACTCATTGGGTGCTTCTCCTACTTGGACTACCGCACAGTTGTCCATGGGATTCGCTCTCATCTTTTGCATGGTTCATCTCCTGGTGGGAAAGCACGGTCAGGATTTCTTGATCAGGGGAATGAAAGTTGTGATGTGTTTGATGGGAATTGGGTTTGGGATGAGAGCTATCCTCTGTATCAGTCCCAGAATTGCTCATTTTTAGATGGAGGGTTTAGGTGTACTGAGAATGGGAGGCCTGATAGCTTCTATACCAAATGGCGTTGGCAACCCAAAGATTGCAACTTGCCCAGATTTGATGCAGGGATGATGTTGGAAAAGCTTCGAAACCGACGTCTTGTATTTGCTGGTGACTCGATTGGAAGGAACCAGTGGGAGTCTCTTCTCTGCATGCTCTCTTTTGTAATTCCTAACAAGACTTCAATCTATGAGGTGAATGGGAGGCCAATCACGAAGCACTCAGGGTTTTTGGTCTTTAAGTTTGAGGACTTCAACTGCACAGTGGAATACTACAGGTCACCTTTTCTAGTCTTTCAGGGCCATCCCCCGGCTGGCGCCCCTGAGGAGGTGAAGTTGGCTTTAAAAGTGGATAAAATGGACTGGATTTCCCGCCACTGGAGGGATGCAGATGTGCTGGTTTTGAATGCTGGGCACTGGTGGAATTATGGGAAGACCATAAGAGAGGGTTGCTACTTTCAAGAAGGGGAGGAGATAAAGATGAATATGACTGTTAAAACTGCATACCAGAGATCAATGGAAACTTTGCTTGATTGGATTGATAGTGAAGTCAACATAAGCAAGACTTATGTACTTTTTCGAACTTATGCTCCTGTGCATTTCAGTGGTGGTGATTGGAATGCTGGTGGTGGTTGTCACTTGGAAACTCTACCTGGATTAGGCCCATTGCCTGTTTTATCCGACAGTGTTTACAAGACTATCTTTGATGTGTTATCTGAGCGGTCAAATGAGTCGCATGTAAGAAAATTCGATTTTCTGAATGTAACAAACATGTCTCTATGGAGAAAAGATGGCCACGCATCTCTGTACTATCTAGGGCCTAATGGAGCAGCCCCTATCAACCATCAAGACTGCAGCCACTGGTGCCTACCTGGTGTCCCTGATGCATGGAACGAACTTCTGTATGCCCTTTTCCTAAAACAGGAATCCATTCGCTCAGGAAATTCAACCAAATCTTCTCAGCTTCCGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

433

Amino Acids

49.57

Weight (kDa)

6.7

Isoelectric Point (pI)

49.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 72 - 125 8.9e-23 PMR5 N terminal Domain
PC-Esterase PF13839 126 - 412 2.8e-95 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 427
AccB1I GGYRCC 2 cut(s) 644, 1191
AccBSI CCGCTC 1 cut(s) 1048
AciI CCGC 3 cut(s) 124, 700, 1048
AcoI YGGCCR 1 cut(s) 1120
AcsI RAATTY 2 cut(s) 1073, 1270
AcyI GRCGYC 2 cut(s) 424, 645
AfaI GTAC 3 cut(s) 313, 900, 1136
AfeI AGCGCT 1 cut(s) 35
AfiI CCNNNNNNNGG 8 cut(s) 59, 637, 641, 655, 705, 709, 986, 1249
AflIII ACRYGT 1 cut(s) 1098
AgsI TTSAA 7 cut(s) 22, 40, 513, 583, 733, 788, 1275
AjnI CCWGG 3 cut(s) 172, 979, 1198
AloI GAACNNNNNNTCC 2 cut(s) 901, 933
AluBI AGCT 4 cut(s) 258, 336, 412, 1291
AluI AGCT 4 cut(s) 258, 336, 412, 1291
Alw26I GTCTC 2 cut(s) 474, 1107
AlwNI CAGNNNCTG 1 cut(s) 1188
Aor51HI AGCGCT 1 cut(s) 35
AoxI GGCC 6 cut(s) 326, 533, 628, 988, 1120, 1145
ApeKI GCWGC 2 cut(s) 1157, 1182
ApoI RAATTY 2 cut(s) 1073, 1270
ArsI GACNNNNNNTTYG 2 cut(s) 409, 441
Asp700I GAANNNNTTC 2 cut(s) 411, 1257
AspLEI GCGC 2 cut(s) 36, 647
AspS9I GGNCC 3 cut(s) 628, 989, 1145
AsuC2I CCSGG 1 cut(s) 638
AsuHPI GGTGA 5 cut(s) 452, 535, 600, 670, 950
AsuII TTCGAA 2 cut(s) 415, 907
AxyI CCTNAGG 1 cut(s) 651
BaeGI GKGCMC 1 cut(s) 744
BalI TGGCCA 1 cut(s) 1122
BanI GGYRCC 2 cut(s) 644, 1191
BbsI GAAGAC 1 cut(s) 767
BbvI GCAGC 2 cut(s) 1169, 1194
BccI CCATC 5 cut(s) 227, 290, 639, 1112, 1179
BciT130I CCWGG 3 cut(s) 174, 981, 1200
BclI TGATCA 1 cut(s) 202
BcnI CCSGG 1 cut(s) 638
BcoDI GTCTC 2 cut(s) 474, 1107
BfaI CTAG 3 cut(s) 47, 617, 1142
BfmI CTRYAG 2 cut(s) 601, 1180
BfoI RGCGCY 2 cut(s) 37, 648
BisI GCNGC 2 cut(s) 1158, 1183
BlsI GCNGC 2 cut(s) 1159, 1184
Bme1390I CCNGG 4 cut(s) 174, 638, 981, 1200
BmgT120I GGNCC 3 cut(s) 628, 989, 1145
BmiI GGNNCC 3 cut(s) 458, 646, 1193
BmrFI CCNGG 4 cut(s) 174, 638, 981, 1200
BmsI GCATC 4 cut(s) 379, 703, 1136, 1201
BpiI GAAGAC 1 cut(s) 767
BplI GAGNNNNNCTC 2 cut(s) 247, 279
BpmI CTGGAG 1 cut(s) 727
Bpu10I CCTNAGC 1 cut(s) 1264
Bpu14I TTCGAA 2 cut(s) 415, 907
BpuMI CCSGG 1 cut(s) 638
BsaHI GRCGYC 2 cut(s) 424, 645
BsaJI CCNNGG 2 cut(s) 136, 636
BsaXI ACNNNNNCTCC 4 cut(s) 647, 677, 901, 931
Bsc4I CCNNNNNNNGG 8 cut(s) 59, 637, 641, 655, 705, 709, 986, 1249
Bse1I ACTGG 5 cut(s) 460, 695, 710, 749, 1193
Bse21I CCTNAGG 1 cut(s) 651
Bse3DI GCAATG 1 cut(s) 992
BseBI CCWGG 3 cut(s) 174, 981, 1200
BseDI CCNNGG 2 cut(s) 136, 636
BseGI GGATG 4 cut(s) 256, 402, 631, 718
BseLI CCNNNNNNNGG 8 cut(s) 59, 637, 641, 655, 705, 709, 986, 1249
BseMI GCAATG 1 cut(s) 992
BseMII CTCAG 6 cut(s) 306, 564, 642, 1035, 1278, 1301
BseNI ACTGG 5 cut(s) 460, 695, 710, 749, 1193
BseRI GAGGAG 2 cut(s) 668, 812
BseSI GKGCMC 1 cut(s) 744
BseXI GCAGC 2 cut(s) 1169, 1194
BseYI CCCAGC 1 cut(s) 737
BsgI GTGCAG 1 cut(s) 571
BshFI GGCC 6 cut(s) 328, 535, 630, 990, 1122, 1147
BshNI GGYRCC 2 cut(s) 644, 1191
BsiSI CCGG 1 cut(s) 638
BslFI GGGAC 2 cut(s) 259, 1190
BslI CCNNNNNNNGG 8 cut(s) 59, 637, 641, 655, 705, 709, 986, 1249
BsmAI GTCTC 2 cut(s) 474, 1107
BsmFI GGGAC 2 cut(s) 259, 1190
BsmI GAATGC 3 cut(s) 63, 739, 952
BsnI GGCC 6 cut(s) 328, 535, 630, 990, 1122, 1147
Bsp119I TTCGAA 2 cut(s) 415, 907
Bsp1286I GDGCHC 1 cut(s) 744
Bsp143I GATC 2 cut(s) 202, 839
Bsp19I CCATGG 1 cut(s) 136
BspACI CCGC 3 cut(s) 124, 700, 1048
BspANI GGCC 6 cut(s) 328, 535, 630, 990, 1122, 1147
BspCNI CTCAG 6 cut(s) 307, 563, 643, 1036, 1277, 1300
BspLI GGNNCC 3 cut(s) 458, 646, 1193
BspMAI CTGCAG 1 cut(s) 1184
BspT104I TTCGAA 2 cut(s) 415, 907
BspT107I GGYRCC 2 cut(s) 644, 1191
BsrBI CCGCTC 1 cut(s) 1048
BsrDI GCAATG 1 cut(s) 992
BsrI ACTGG 5 cut(s) 460, 695, 710, 749, 1193
BssECI CCNNGG 2 cut(s) 136, 636
BssMI GATC 2 cut(s) 202, 839
BssNI GRCGYC 2 cut(s) 424, 645
BssT1I CCWWGG 1 cut(s) 136
Bst2UI CCWGG 3 cut(s) 174, 981, 1200
Bst4CI ACNGT 6 cut(s) 88, 130, 189, 592, 820, 1013
Bst6I CTCTTC 1 cut(s) 477
BstACI GRCGYC 2 cut(s) 424, 645
BstBI TTCGAA 2 cut(s) 415, 907
BstC8I GCNNGC 2 cut(s) 482, 643
BstDEI CTNAG 6 cut(s) 315, 550, 651, 1044, 1264, 1287
BstDSI CCRYGG 1 cut(s) 136
BstEII GGTNACC 1 cut(s) 606
BstENI CCTNNNNNAGG 2 cut(s) 984, 1247
BstF5I GGATG 4 cut(s) 256, 402, 631, 718
BstH2I RGCGCY 2 cut(s) 37, 648
BstHHI GCGC 2 cut(s) 36, 647
BstKTI GATC 2 cut(s) 205, 842
BstMAI GTCTC 2 cut(s) 474, 1107
BstMBI GATC 2 cut(s) 202, 839
BstMWI GCNNNNNNNGC 2 cut(s) 69, 1191
BstNI CCWGG 3 cut(s) 174, 981, 1200
BstNSI RCATGY 3 cut(s) 484, 1067, 1102
BstPI GGTNACC 1 cut(s) 606
BstSCI CCNGG 4 cut(s) 172, 636, 979, 1198
BstSFI CTRYAG 2 cut(s) 601, 1180
BstSLI GKGCMC 1 cut(s) 744
BstV1I GCAGC 2 cut(s) 1169, 1194
BstV2I GAAGAC 1 cut(s) 767
Bsu36I CCTNAGG 1 cut(s) 651
BsuRI GGCC 6 cut(s) 328, 535, 630, 990, 1122, 1147
BtgI CCRYGG 1 cut(s) 136
BtsCI GGATG 4 cut(s) 256, 402, 631, 718
BtsIMutI CAGTG 8 cut(s) 84, 467, 597, 703, 742, 937, 1018, 1186
Cac8I GCNNGC 2 cut(s) 482, 643
CaiI CAGNNNCTG 1 cut(s) 1188
CfoI GCGC 2 cut(s) 36, 647
Cfr13I GGNCC 3 cut(s) 628, 989, 1145
CsiI ACCWGGT 1 cut(s) 1198
Csp6I GTAC 3 cut(s) 312, 899, 1135
CviAII CATG 6 cut(s) 137, 161, 481, 1064, 1099, 1215
CviQI GTAC 3 cut(s) 312, 899, 1135
DdeI CTNAG 6 cut(s) 315, 550, 651, 1044, 1264, 1287
DinI GGCGCC 1 cut(s) 646
DpnI GATC 2 cut(s) 204, 841
DpnII GATC 2 cut(s) 202, 839
DraI TTTAAA 1 cut(s) 672
EaeI YGGCCR 1 cut(s) 1120
Eam1104I CTCTTC 1 cut(s) 477
EarI CTCTTC 1 cut(s) 477
Eco130I CCWWGG 1 cut(s) 136
Eco147I AGGCCT 1 cut(s) 328
Eco47III AGCGCT 1 cut(s) 35
Eco81I CCTNAGG 1 cut(s) 651
Eco91I GGTNACC 1 cut(s) 606
EcoNI CCTNNNNNAGG 2 cut(s) 984, 1247
EcoO109I RGGNCCY 1 cut(s) 1145
EcoO65I GGTNACC 1 cut(s) 606
EcoRII CCWGG 3 cut(s) 172, 979, 1198
EcoT14I CCWWGG 1 cut(s) 136
EcoT22I ATGCAT 1 cut(s) 1216
EgeI GGCGCC 1 cut(s) 646
EheI GGCGCC 1 cut(s) 646
ErhI CCWWGG 1 cut(s) 136
FaeI CATG 6 cut(s) 140, 164, 484, 1067, 1102, 1218
FalI AAGNNNNNCTT 4 cut(s) 877, 909, 1013, 1045
FaqI GGGAC 2 cut(s) 259, 1190
FatI CATG 6 cut(s) 136, 160, 480, 1063, 1098, 1214
FauI CCCGC 1 cut(s) 707
FbaI TGATCA 1 cut(s) 202
Fnu4HI GCNGC 2 cut(s) 1158, 1183
FokI GGATG 4 cut(s) 263, 409, 618, 725
Fsp4HI GCNGC 2 cut(s) 1158, 1183
FspBI CTAG 3 cut(s) 47, 617, 1142
GlaI GCGC 2 cut(s) 35, 646
GluI GCNGC 2 cut(s) 1158, 1183
GsaI CCCAGC 1 cut(s) 741
GsuI CTGGAG 1 cut(s) 727
HaeII RGCGCY 2 cut(s) 37, 648
HaeIII GGCC 6 cut(s) 328, 535, 630, 990, 1122, 1147
HapII CCGG 1 cut(s) 638
HhaI GCGC 2 cut(s) 36, 647
Hin1I GRCGYC 2 cut(s) 424, 645
Hin1II CATG 6 cut(s) 140, 164, 484, 1067, 1102, 1218
Hin6I GCGC 2 cut(s) 34, 645
HinP1I GCGC 2 cut(s) 34, 645
HincII GTYRAC 1 cut(s) 880
HindII GTYRAC 1 cut(s) 880
HindIII AAGCTT 1 cut(s) 410
HinfI GANTC 5 cut(s) 142, 443, 467, 1058, 1253
HpaII CCGG 1 cut(s) 638
HphI GGTGA 5 cut(s) 452, 535, 600, 670, 950
Hpy166II GTNNAC 3 cut(s) 312, 880, 1018
Hpy188I TCNGA 3 cut(s) 1009, 1045, 1086
Hpy188III TCNNGA 8 cut(s) 22, 47, 192, 200, 541, 788, 1175, 1266
Hpy8I GTNNAC 3 cut(s) 312, 880, 1018
Hpy99I CGWCG 1 cut(s) 426
HpyAV CCTTC 3 cut(s) 63, 447, 785
HpyCH4III ACNGT 6 cut(s) 88, 130, 189, 592, 820, 1013
HpyCH4IV ACGT 1 cut(s) 424
HpyF10VI GCNNNNNNNGC 2 cut(s) 69, 1191
HpyF3I CTNAG 6 cut(s) 315, 550, 651, 1044, 1264, 1287
HpySE526I ACGT 1 cut(s) 424
Hsp92I GRCGYC 2 cut(s) 424, 645
Hsp92II CATG 6 cut(s) 140, 164, 484, 1067, 1102, 1218
HspAI GCGC 2 cut(s) 34, 645
KasI GGCGCC 1 cut(s) 644
Ksp22I TGATCA 1 cut(s) 202
Kzo9I GATC 2 cut(s) 202, 839
LmnI GCTCC 2 cut(s) 922, 1154
Lsp1109I GCAGC 2 cut(s) 1169, 1194
LweI GCATC 4 cut(s) 379, 703, 1136, 1201
MabI ACCWGGT 1 cut(s) 1198
MaeI CTAG 3 cut(s) 47, 617, 1142
MaeII ACGT 1 cut(s) 424
MaeIII GTNAC 5 cut(s) 82, 440, 606, 962, 1090
MalI GATC 2 cut(s) 204, 841
MbiI CCGCTC 1 cut(s) 1048
MboI GATC 2 cut(s) 202, 839
MboII GAAGA 5 cut(s) 10, 67, 464, 772, 1275
MhlI GDGCHC 1 cut(s) 744
MlsI TGGCCA 1 cut(s) 1122
MluCI AATT 6 cut(s) 238, 280, 495, 751, 1073, 1270
MluNI TGGCCA 1 cut(s) 1122
Mly113I GGCGCC 1 cut(s) 645
MlyI GAGTC 3 cut(s) 437, 476, 1067
MmeI TCCRAC 2 cut(s) 384, 1032
Mox20I TGGCCA 1 cut(s) 1122
Mph1103I ATGCAT 1 cut(s) 1216
MroXI GAANNNNTTC 2 cut(s) 411, 1257
MscI TGGCCA 1 cut(s) 1122
MseI TTAA 3 cut(s) 567, 671, 822
MslI CAYNNNNRTG 1 cut(s) 930
Msp20I TGGCCA 1 cut(s) 1122
MspI CCGG 1 cut(s) 638
MspR9I CCNGG 4 cut(s) 174, 638, 981, 1200
Mva1269I GAATGC 3 cut(s) 63, 739, 952
MvaI CCWGG 3 cut(s) 174, 981, 1200
MwoI GCNNNNNNNGC 2 cut(s) 69, 1191
NarI GGCGCC 1 cut(s) 645
NciI CCSGG 1 cut(s) 638
NcoI CCATGG 1 cut(s) 136
NdeII GATC 2 cut(s) 202, 839
NlaIII CATG 6 cut(s) 140, 164, 484, 1067, 1102, 1218
NlaIV GGNNCC 3 cut(s) 458, 646, 1193
NmuCI GTSAC 4 cut(s) 82, 440, 606, 962
NsiI ATGCAT 1 cut(s) 1216
NspI RCATGY 3 cut(s) 484, 1067, 1102
NspV TTCGAA 2 cut(s) 415, 907
PaeI GCATGC 1 cut(s) 484
PceI AGGCCT 1 cut(s) 328
PciI ACATGT 1 cut(s) 1098
PcsI WCGNNNNNNNCGW 1 cut(s) 421
PctI GAATGC 3 cut(s) 63, 739, 952
PdmI GAANNNNTTC 2 cut(s) 411, 1257
PfeI GAWTC 2 cut(s) 142, 1253
PkrI GCNGC 2 cut(s) 1159, 1184
PleI GAGTC 3 cut(s) 437, 475, 1066
PluTI GGCGCC 1 cut(s) 648
PpsI GAGTC 3 cut(s) 437, 475, 1066
PscI ACATGT 1 cut(s) 1098
Psp6I CCWGG 3 cut(s) 172, 979, 1198
PspEI GGTNACC 1 cut(s) 606
PspFI CCCAGC 1 cut(s) 737
PspGI CCWGG 3 cut(s) 172, 979, 1198
PspN4I GGNNCC 3 cut(s) 458, 646, 1193
PspPI GGNCC 3 cut(s) 628, 989, 1145
PstI CTGCAG 1 cut(s) 1184
PstNI CAGNNNCTG 1 cut(s) 1188
RsaI GTAC 3 cut(s) 313, 900, 1136
RsaNI GTAC 3 cut(s) 312, 899, 1135
RseI CAYNNNNRTG 1 cut(s) 930
SaqAI TTAA 3 cut(s) 567, 671, 822
SatI GCNGC 2 cut(s) 1158, 1183
Sau3AI GATC 2 cut(s) 202, 839
Sau96I GGNCC 3 cut(s) 628, 989, 1145
SchI GAGTC 3 cut(s) 437, 476, 1067
ScrFI CCNGG 4 cut(s) 174, 638, 981, 1200
SduI GDGCHC 1 cut(s) 744
SexAI ACCWGGT 1 cut(s) 1198
SfaNI GCATC 4 cut(s) 379, 703, 1136, 1201
SfcI CTRYAG 2 cut(s) 601, 1180
SfoI GGCGCC 1 cut(s) 646
SfuI TTCGAA 2 cut(s) 415, 907
SmiMI CAYNNNNRTG 1 cut(s) 930
SphI GCATGC 1 cut(s) 484
Sse9I AATT 6 cut(s) 238, 280, 495, 751, 1073, 1270
SseBI AGGCCT 1 cut(s) 328
SsiI CCGC 3 cut(s) 124, 700, 1048
SspDI GGCGCC 1 cut(s) 644
SspMI CTAG 3 cut(s) 47, 617, 1142
StuI AGGCCT 1 cut(s) 328
StyD4I CCNGG 4 cut(s) 172, 636, 979, 1198
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 6 cut(s) 88, 130, 189, 592, 820, 1013
TaiI ACGT 1 cut(s) 427
TaqI TCGA 4 cut(s) 415, 446, 907, 1077
TasI AATT 6 cut(s) 238, 280, 495, 751, 1073, 1270
TatI WGTACW 3 cut(s) 311, 898, 1134
TfiI GAWTC 2 cut(s) 142, 1253
Tru1I TTAA 3 cut(s) 567, 671, 822
Tru9I TTAA 3 cut(s) 567, 671, 822
TscAI CASTG 8 cut(s) 91, 467, 597, 710, 749, 937, 1018, 1193
TseFI GTSAC 4 cut(s) 82, 440, 606, 962
TseI GCWGC 2 cut(s) 1157, 1182
Tsp45I GTSAC 4 cut(s) 82, 440, 606, 962
TspDTI ATGAA 3 cut(s) 156, 228, 824
TspGWI ACGGA 1 cut(s) 1284
TspRI CASTG 8 cut(s) 91, 467, 597, 710, 749, 937, 1018, 1193
XagI CCTNNNNNAGG 2 cut(s) 984, 1247
XapI RAATTY 2 cut(s) 1073, 1270
XbaI TCTAGA 1 cut(s) 46
XceI RCATGY 3 cut(s) 484, 1067, 1102
XcmI CCANNNNNNNNNTGG 1 cut(s) 638
XmnI GAANNNNTTC 2 cut(s) 411, 1257
XspI CTAG 3 cut(s) 47, 617, 1142
ZraI GACGTC 1 cut(s) 425
Zsp2I ATGCAT 1 cut(s) 1216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.