FvH4_2g37390

Glutelin type-A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001439
Physical Location & Seq
Forward (+)
27271458 .. 27272835
1378 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g37390.t1

Sequence Viewer

Length: 1068 bp
ATGGAGATTGATCTTACACCAAGGTTGGCTAAGCAGGTTTATGGAGGAGATGGTGGTTCCTACTCTGCATGGTCCCCATCGGAGCTTCCCATGCTACGTGAAGGTGACATCGGAGCTGCCAAGCTCTCTCTGGAGAAGGACGGCTTCGCTCTCCCCAATTACTCTGACTCTGCCAGAGTTGCTTATGTCCTTCAAGGTAATGGAGTAGTCGGGATTGTTTTGCCCGAGAAGGAAGAGAAGGTTCTGCCTGTTAAGAAGGGTGATGCTCTTGCCCTCCCCTTTGGTGTCATCACATGGTGGCACAACAAGGAGGATACCGAGTTTGTGGTTCTTTTCTTGGGTGACACCAAAACCGCTCACAAAAGAGGAGAGTTCACCGATATGTATCTCAATGGTTCCAATGGAATCTTCACCGGCTTCTCAACTGAGTTTGTCAGCCGAGCATGGGATTTGGAGGAGAGTGTTGTGAAAACTCTTGTTGGCAACCAAAGCGGGAAGGGCATTGTTAAGTTGGGAGGAGCTAATCTGCCTGAACCAAACAAGGAACATAGAGATGGGATGACATTGAACTGCGAAGAGGCTCCTCTTGATGTTGATATTGAGGGAGGTGGAAGAGTTGTTGTTTTGAACACTAAGAACCTACCTCTGGTGGGTGAGGTTGGCCTTGGCGCCGATCTTGTGAGATTGGATGGTAGTGCTATGTGCTCTCCTGGGTTTTCTTGTGACTCTGCGTTGCAGGTCACTTACATTGTTAGGGGCAGTGGCCGTGTCCAAGCTGTAGGTGTCGATGGGAAGAGGGTCTTGGAAACAACTGTTACGGCTGGTAACTTGTTCATTGTTCCTCGATTCTTTGTGGTTTCAAAGATTGCTGACCCGGAAGGCTTGGAATGGTTCTCCATCATCACCACTCCCAATCCAATTTTCACTCATTTGGCTGGAAGTATTAGTGCTTGGAAAGCGTTATCTCCTCAGGTGCTTGAAGCTTCTTTCAATGTGGATTCGGATACTGAAAAACTCTTCCGATCAAAGAGAACTGCTGATGCAATCTTCTTCCCTCCTCCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

356

Amino Acids

38.25

Weight (kDa)

5.07

Isoelectric Point (pI)

33.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 11 - 156 3.1e-22 Cupin
Cupin_1 PF00190 198 - 337 1.7e-22 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 25, 727
AccB1I GGYRCC 1 cut(s) 668
AccBSI CCGCTC 1 cut(s) 356
AciI CCGC 2 cut(s) 354, 492
AcoI YGGCCR 1 cut(s) 763
AcyI GRCGYC 1 cut(s) 669
AdeI CACNNNGTG 1 cut(s) 297
AfiI CCNNNNNNNGG 3 cut(s) 445, 646, 650
AgsI TTSAA 6 cut(s) 194, 568, 628, 861, 980, 991
AjnI CCWGG 1 cut(s) 709
AjuI GAANNNNNNNTTGG 6 cut(s) 392, 424, 785, 817, 905, 937
AluBI AGCT 6 cut(s) 85, 116, 124, 521, 776, 983
AluI AGCT 6 cut(s) 85, 116, 124, 521, 776, 983
Alw21I GWGCWC 1 cut(s) 707
Ama87I CYCGRG 1 cut(s) 224
AoxI GGCC 2 cut(s) 661, 763
ApeKI GCWGC 1 cut(s) 116
AspLEI GCGC 1 cut(s) 671
AspS9I GGNCC 1 cut(s) 72
AsuC2I CCSGG 1 cut(s) 875
AsuHPI GGTGA 7 cut(s) 116, 272, 353, 367, 403, 665, 895
AvaI CYCGRG 1 cut(s) 224
AvaII GGWCC 1 cut(s) 72
AxyI CCTNAGG 1 cut(s) 969
BanI GGYRCC 1 cut(s) 668
Bbv12I GWGCWC 1 cut(s) 707
BbvI GCAGC 1 cut(s) 103
BccI CCATC 6 cut(s) 44, 85, 548, 683, 782, 905
BceAI ACGGC 3 cut(s) 157, 750, 834
BciT130I CCWGG 1 cut(s) 711
BciVI GTATCC 2 cut(s) 307, 997
BcnI CCSGG 1 cut(s) 875
BfmI CTRYAG 1 cut(s) 777
BfoI RGCGCY 1 cut(s) 672
BfuAI ACCTGC 2 cut(s) 25, 727
BfuI GTATCC 2 cut(s) 307, 997
BisI GCNGC 1 cut(s) 117
BlpI GCTNAGC 1 cut(s) 30
BlsI GCNGC 1 cut(s) 118
Bme1390I CCNGG 2 cut(s) 711, 875
Bme18I GGWCC 1 cut(s) 72
BmeT110I CYCGRG 1 cut(s) 224
BmgT120I GGNCC 1 cut(s) 72
BmiI GGNNCC 5 cut(s) 58, 74, 397, 582, 670
BmrFI CCNGG 2 cut(s) 711, 875
BmsI GCATC 2 cut(s) 253, 1030
BpmI CTGGAG 1 cut(s) 152
Bpu1102I GCTNAGC 1 cut(s) 30
BpuMI CCSGG 1 cut(s) 875
BsaAI YACGTR 1 cut(s) 98
BsaBI GATNNNNATC 1 cut(s) 384
BsaHI GRCGYC 1 cut(s) 669
BsaJI CCNNGG 3 cut(s) 20, 664, 710
Bsc4I CCNNNNNNNGG 3 cut(s) 445, 646, 650
Bse118I RCCGGY 1 cut(s) 413
Bse21I CCTNAGG 1 cut(s) 969
Bse8I GATNNNNATC 1 cut(s) 384
BseBI CCWGG 1 cut(s) 711
BseDI CCNNGG 3 cut(s) 20, 664, 710
BseGI GGATG 2 cut(s) 564, 694
BseJI GATNNNNATC 1 cut(s) 384
BseLI CCNNNNNNNGG 3 cut(s) 445, 646, 650
BseMII CTCAG 2 cut(s) 417, 983
BseRI GAGGAG 7 cut(s) 60, 381, 470, 531, 573, 957, 1047
BseXI GCAGC 1 cut(s) 103
BshFI GGCC 2 cut(s) 663, 765
BshNI GGYRCC 1 cut(s) 668
BsiHKAI GWGCWC 1 cut(s) 707
BsiHKCI CYCGRG 1 cut(s) 224
BsiSI CCGG 2 cut(s) 414, 875
BslFI GGGAC 1 cut(s) 58
BslI CCNNNNNNNGG 3 cut(s) 445, 646, 650
BsmFI GGGAC 1 cut(s) 58
BsnI GGCC 2 cut(s) 663, 765
BsoBI CYCGRG 1 cut(s) 224
Bsp1286I GDGCHC 1 cut(s) 707
Bsp143I GATC 3 cut(s) 10, 673, 1022
Bsp1720I GCTNAGC 1 cut(s) 30
BspACI CCGC 2 cut(s) 354, 492
BspANI GGCC 2 cut(s) 663, 765
BspCNI CTCAG 2 cut(s) 418, 982
BspLI GGNNCC 5 cut(s) 58, 74, 397, 582, 670
BspMI ACCTGC 2 cut(s) 25, 727
BspT107I GGYRCC 1 cut(s) 668
BsrBI CCGCTC 1 cut(s) 356
BsrFI RCCGGY 1 cut(s) 413
BssAI RCCGGY 1 cut(s) 413
BssECI CCNNGG 3 cut(s) 20, 664, 710
BssMI GATC 3 cut(s) 10, 673, 1022
BssNI GRCGYC 1 cut(s) 669
BssT1I CCWWGG 2 cut(s) 20, 664
Bst2UI CCWGG 1 cut(s) 711
Bst4CI ACNGT 1 cut(s) 814
Bst6I CTCTTC 5 cut(s) 228, 570, 607, 788, 1022
BstACI GRCGYC 1 cut(s) 669
BstBAI YACGTR 1 cut(s) 98
BstDEI CTNAG 4 cut(s) 30, 426, 633, 969
BstF5I GGATG 2 cut(s) 564, 694
BstH2I RGCGCY 1 cut(s) 672
BstHHI GCGC 1 cut(s) 671
BstKTI GATC 3 cut(s) 13, 676, 1025
BstMBI GATC 3 cut(s) 10, 673, 1022
BstMWI GCNNNNNNNGC 5 cut(s) 91, 179, 489, 498, 956
BstNI CCWGG 1 cut(s) 711
BstSCI CCNGG 2 cut(s) 709, 873
BstSFI CTRYAG 1 cut(s) 777
BstV1I GCAGC 1 cut(s) 103
Bsu36I CCTNAGG 1 cut(s) 969
BsuI GTATCC 2 cut(s) 307, 997
BsuRI GGCC 2 cut(s) 663, 765
BtsCI GGATG 2 cut(s) 564, 694
BtsI GCAGTG 1 cut(s) 766
BtsIMutI CAGTG 1 cut(s) 766
BveI ACCTGC 2 cut(s) 25, 727
CfoI GCGC 1 cut(s) 671
Cfr10I RCCGGY 1 cut(s) 413
Cfr13I GGNCC 1 cut(s) 72
CviAII CATG 4 cut(s) 69, 91, 294, 444
DdeI CTNAG 4 cut(s) 30, 426, 633, 969
DinI GGCGCC 1 cut(s) 670
DpnI GATC 3 cut(s) 12, 675, 1024
DpnII GATC 3 cut(s) 10, 673, 1022
DraIII CACNNNGTG 1 cut(s) 297
EaeI YGGCCR 1 cut(s) 763
Eam1104I CTCTTC 5 cut(s) 228, 570, 607, 788, 1022
EarI CTCTTC 5 cut(s) 228, 570, 607, 788, 1022
Eco130I CCWWGG 2 cut(s) 20, 664
Eco47I GGWCC 1 cut(s) 72
Eco81I CCTNAGG 1 cut(s) 969
Eco88I CYCGRG 1 cut(s) 224
EcoRII CCWGG 1 cut(s) 709
EcoT14I CCWWGG 2 cut(s) 20, 664
EgeI GGCGCC 1 cut(s) 670
EheI GGCGCC 1 cut(s) 670
ErhI CCWWGG 2 cut(s) 20, 664
FaeI CATG 4 cut(s) 72, 94, 297, 447
FaiI YATR 9 cut(s) 42, 70, 92, 186, 295, 383, 445, 549, 701
FalI AAGNNNNNCTT 4 cut(s) 128, 160, 785, 817
FaqI GGGAC 1 cut(s) 58
FatI CATG 4 cut(s) 68, 90, 293, 443
FauI CCCGC 1 cut(s) 485
Fnu4HI GCNGC 1 cut(s) 117
FokI GGATG 2 cut(s) 571, 701
Fsp4HI GCNGC 1 cut(s) 117
GlaI GCGC 1 cut(s) 670
GluI GCNGC 1 cut(s) 117
GsuI CTGGAG 1 cut(s) 152
HaeII RGCGCY 1 cut(s) 672
HaeIII GGCC 2 cut(s) 663, 765
HapII CCGG 2 cut(s) 414, 875
HhaI GCGC 1 cut(s) 671
Hin1I GRCGYC 1 cut(s) 669
Hin1II CATG 4 cut(s) 72, 94, 297, 447
Hin6I GCGC 1 cut(s) 669
HinP1I GCGC 1 cut(s) 669
HindIII AAGCTT 1 cut(s) 981
HinfI GANTC 5 cut(s) 167, 405, 725, 846, 998
HpaII CCGG 2 cut(s) 414, 875
HphI GGTGA 7 cut(s) 116, 272, 353, 367, 403, 665, 895
Hpy166II GTNNAC 1 cut(s) 375
Hpy188I TCNGA 5 cut(s) 82, 113, 166, 1003, 1022
Hpy188III TCNNGA 3 cut(s) 131, 211, 587
Hpy8I GTNNAC 1 cut(s) 375
HpyAV CCTTC 8 cut(s) 95, 130, 200, 223, 232, 250, 490, 872
HpyCH4III ACNGT 1 cut(s) 814
HpyCH4IV ACGT 1 cut(s) 97
HpyCH4V TGCA 3 cut(s) 68, 736, 1043
HpyF10VI GCNNNNNNNGC 5 cut(s) 91, 179, 489, 498, 956
HpyF3I CTNAG 4 cut(s) 30, 426, 633, 969
HpySE526I ACGT 1 cut(s) 97
Hsp92I GRCGYC 1 cut(s) 669
Hsp92II CATG 4 cut(s) 72, 94, 297, 447
HspAI GCGC 1 cut(s) 669
KasI GGCGCC 1 cut(s) 668
Kzo9I GATC 3 cut(s) 10, 673, 1022
LmnI GCTCC 4 cut(s) 82, 113, 518, 586
Lsp1109I GCAGC 1 cut(s) 103
LweI GCATC 2 cut(s) 253, 1030
MaeII ACGT 1 cut(s) 97
MaeIII GTNAC 6 cut(s) 104, 341, 722, 739, 814, 824
MalI GATC 3 cut(s) 12, 675, 1024
MbiI CCGCTC 1 cut(s) 356
MboI GATC 3 cut(s) 10, 673, 1022
MboII GAAGA 8 cut(s) 245, 400, 587, 624, 805, 1009, 1039, 1042
MhlI GDGCHC 1 cut(s) 707
MluCI AATT 2 cut(s) 157, 918
Mly113I GGCGCC 1 cut(s) 669
MlyI GAGTC 2 cut(s) 161, 719
MseI TTAA 2 cut(s) 252, 507
MslI CAYNNNNRTG 2 cut(s) 380, 552
MspI CCGG 2 cut(s) 414, 875
MspR9I CCNGG 2 cut(s) 711, 875
MvaI CCWGG 1 cut(s) 711
MwoI GCNNNNNNNGC 5 cut(s) 91, 179, 489, 498, 956
NarI GGCGCC 1 cut(s) 669
NciI CCSGG 1 cut(s) 875
NdeII GATC 3 cut(s) 10, 673, 1022
NlaIII CATG 4 cut(s) 72, 94, 297, 447
NlaIV GGNNCC 5 cut(s) 58, 74, 397, 582, 670
NmeAIII GCCGAG 1 cut(s) 464
NmuCI GTSAC 4 cut(s) 104, 341, 722, 739
PfeI GAWTC 3 cut(s) 405, 846, 998
PkrI GCNGC 1 cut(s) 118
PleI GAGTC 2 cut(s) 161, 719
PluTI GGCGCC 1 cut(s) 672
PpsI GAGTC 2 cut(s) 161, 719
Ppu21I YACGTR 1 cut(s) 98
Psp6I CCWGG 1 cut(s) 709
PspGI CCWGG 1 cut(s) 709
PspN4I GGNNCC 5 cut(s) 58, 74, 397, 582, 670
PspPI GGNCC 1 cut(s) 72
RseI CAYNNNNRTG 2 cut(s) 380, 552
SaqAI TTAA 2 cut(s) 252, 507
SatI GCNGC 1 cut(s) 117
Sau3AI GATC 3 cut(s) 10, 673, 1022
Sau96I GGNCC 1 cut(s) 72
SchI GAGTC 2 cut(s) 161, 719
ScrFI CCNGG 2 cut(s) 711, 875
SduI GDGCHC 1 cut(s) 707
SfaNI GCATC 2 cut(s) 253, 1030
SfcI CTRYAG 1 cut(s) 777
SfoI GGCGCC 1 cut(s) 670
SinI GGWCC 1 cut(s) 72
SmiMI CAYNNNNRTG 2 cut(s) 380, 552
Sse9I AATT 2 cut(s) 157, 918
SsiI CCGC 2 cut(s) 354, 492
SspDI GGCGCC 1 cut(s) 668
StyD4I CCNGG 2 cut(s) 709, 873
StyI CCWWGG 2 cut(s) 20, 664
TaaI ACNGT 1 cut(s) 814
TaiI ACGT 1 cut(s) 100
TaqI TCGA 2 cut(s) 786, 844
TasI AATT 2 cut(s) 157, 918
TfiI GAWTC 3 cut(s) 405, 846, 998
Tru1I TTAA 2 cut(s) 252, 507
Tru9I TTAA 2 cut(s) 252, 507
TscAI CASTG 1 cut(s) 766
TseFI GTSAC 4 cut(s) 104, 341, 722, 739
TseI GCWGC 1 cut(s) 116
Tsp45I GTSAC 4 cut(s) 104, 341, 722, 739
TspDTI ATGAA 1 cut(s) 823
TspRI CASTG 1 cut(s) 766
VpaK11BI GGWCC 1 cut(s) 72
XcmI CCANNNNNNNNNTGG 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.