MD11G1115000.v1.1

Glutelin type-A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0001439
Physical Location & Seq
Forward (+)
10378944 .. 10381361
2418 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1115000.v1.1.491

Sequence Viewer

Length: 1068 bp
ATGGAGCTTGATCTTTCACCCAAGTTGGCCAAGAAGGTGTACGGCGGCGATGGTGGCTCCTACTTTGCCTGGTCACCGTCGGAGCTTCCCATGCTCCGTGAAGGTAACATCGGCGCCGCCAAGCTCGCTCTGGAAAAGGACGGGTTCGCTCTTCCCAAGTACTCTGATTCCAATCAGGTCGCTTATGTCCTCCAAGGCACTGGTGCAGTTGGAATTGTCCTGCCTGAGAAGGAAGAGAAGGTTCTTCCAGTTAAGAAGGGTGATGCCATTGCCCTCCCTTTCGGAGTTGTCACTTGGTGGTACAACAAGGAGGACACTGAGTTCGTTGTTCTTTTCTTGGGCGACACTTCAAAAGCTCACAAGACGGGTGAGTTTACCGACTTCTATCTCAATGGCTCCAACGCCATTTTCACTGGCTTCTCAACTGAGTTTGTTGGTCGGGCATGGGATTTGGAGGAGAGTGTTGTGAAGACTCTTGTTGGCAAGCAATCCGGCAAGGGCATTGTCAAGTTGAGCGGAGTTACCTTGCCCGAGCCAAAGAAGGAACACCGTGATGGTATGACATTGAACTGCGAAGAGGCTCCCCTGGATGTTGACATTAAGGGTGGTGGAAGAGTTGTGGTCTTGAATACCAAAAACCTTCCTTTGGTCGGTGAGGTTGGGCTTGGTGCTGACCTTGTGAGGTTGGACGGAAGTGCCATGTGCTCCCCTGGATTTTCTTGCGACTCGGCTCTCCAGGTGACTTATATCATTAGGGGCAGTGGCCGTGTCCAGGTTGTTGGTGTTGACGGTAAGAGGGTGCTGGAAACTACTATTAAAGCCGGTAATCTCTTCATCGTTCCTAGGTTCTTTGTTGTGTCAAAGATTGCTGATCCAGAGGGCTTGGAATGGTTTTCTATCATCACTACTCCCAACCCGATATTCACCCACTTGGCTGGAAGCATTGGGTGCTGGAAGGCTCTATCTCCTCAGGTGCTCCAGGCTGCGTTCAACGTGGATTCGGAAACAGAGAAACTTTTCCGTTCGAAGAGGACTTCTGATGCAATCTTCTTCCCTCCACCCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

356

Amino Acids

38.35

Weight (kDa)

5.81

Isoelectric Point (pI)

32.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 11 - 156 6.2e-24 Cupin
Cupin_1 PF00190 198 - 338 4.3e-24 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 113
AccBSI CCGCTC 1 cut(s) 516
AciI CCGC 3 cut(s) 45, 117, 516
AclWI GGATC 1 cut(s) 866
AcoI YGGCCR 2 cut(s) 27, 763
AcyI GRCGYC 1 cut(s) 114
AdeI CACNNNGTG 1 cut(s) 297
AfaI GTAC 3 cut(s) 41, 161, 302
AfiI CCNNNNNNNGG 3 cut(s) 646, 650, 772
AgsI TTSAA 4 cut(s) 351, 568, 628, 991
AjnI CCWGG 6 cut(s) 68, 585, 709, 735, 771, 978
AjuI GAANNNNNNNTTGG 4 cut(s) 392, 424, 905, 937
AleI CACNNNNGTG 1 cut(s) 1063
AloI GAACNNNNNNTCC 2 cut(s) 305, 337
AluBI AGCT 4 cut(s) 7, 85, 124, 356
AluI AGCT 4 cut(s) 7, 85, 124, 356
Alw21I GWGCWC 2 cut(s) 707, 978
AlwI GGATC 1 cut(s) 866
Ama87I CYCGRG 1 cut(s) 530
AoxI GGCC 2 cut(s) 27, 763
ApeKI GCWGC 1 cut(s) 983
ArsI GACNNNNNNTTYG 2 cut(s) 305, 337
Asp700I GAANNNNTTC 1 cut(s) 1016
AspA2I CCTAGG 1 cut(s) 842
AspLEI GCGC 1 cut(s) 116
AsuHPI GGTGA 7 cut(s) 9, 66, 272, 380, 665, 751, 916
AsuII TTCGAA 1 cut(s) 1025
AvaI CYCGRG 1 cut(s) 530
AvrII CCTAGG 1 cut(s) 842
AxyI CCTNAGG 1 cut(s) 969
BalI TGGCCA 1 cut(s) 29
BanI GGYRCC 1 cut(s) 113
BbsI GAAGAC 1 cut(s) 476
Bbv12I GWGCWC 2 cut(s) 707, 978
BbvI GCAGC 1 cut(s) 970
BccI CCATC 2 cut(s) 44, 548
BceAI ACGGC 2 cut(s) 58, 750
BciT130I CCWGG 6 cut(s) 70, 587, 711, 737, 773, 980
BfaI CTAG 1 cut(s) 843
BfoI RGCGCY 1 cut(s) 117
BisI GCNGC 3 cut(s) 46, 117, 984
BlnI CCTAGG 1 cut(s) 842
BlsI GCNGC 3 cut(s) 47, 118, 985
BmcAI AGTACT 1 cut(s) 161
Bme1390I CCNGG 6 cut(s) 70, 587, 711, 737, 773, 980
BmeT110I CYCGRG 1 cut(s) 530
BmiI GGNNCC 4 cut(s) 58, 115, 397, 582
BmrFI CCNGG 6 cut(s) 70, 587, 711, 737, 773, 980
BmsI GCATC 2 cut(s) 253, 1030
BpiI GAAGAC 1 cut(s) 476
BpmI CTGGAG 2 cut(s) 719, 962
Bpu14I TTCGAA 1 cut(s) 1025
BsaBI GATNNNNATC 1 cut(s) 171
BsaHI GRCGYC 1 cut(s) 114
BsaJI CCNNGG 4 cut(s) 193, 585, 709, 842
Bsc4I CCNNNNNNNGG 3 cut(s) 646, 650, 772
Bse118I RCCGGY 1 cut(s) 821
Bse1I ACTGG 3 cut(s) 205, 248, 418
Bse21I CCTNAGG 1 cut(s) 969
Bse3DI GCAATG 1 cut(s) 267
Bse8I GATNNNNATC 1 cut(s) 171
BseBI CCWGG 6 cut(s) 70, 587, 711, 737, 773, 980
BseDI CCNNGG 4 cut(s) 193, 585, 709, 842
BseGI GGATG 1 cut(s) 595
BseJI GATNNNNATC 1 cut(s) 171
BseLI CCNNNNNNNGG 3 cut(s) 646, 650, 772
BseMI GCAATG 1 cut(s) 267
BseMII CTCAG 4 cut(s) 216, 309, 417, 983
BseNI ACTGG 3 cut(s) 205, 248, 418
BseRI GAGGAG 2 cut(s) 470, 957
BseXI GCAGC 1 cut(s) 970
BsgI GTGCAG 1 cut(s) 225
BshFI GGCC 2 cut(s) 29, 765
BshNI GGYRCC 1 cut(s) 113
BsiHKAI GWGCWC 2 cut(s) 707, 978
BsiHKCI CYCGRG 1 cut(s) 530
BsiSI CCGG 2 cut(s) 492, 822
BslI CCNNNNNNNGG 3 cut(s) 646, 650, 772
BsnI GGCC 2 cut(s) 29, 765
BsoBI CYCGRG 1 cut(s) 530
Bsp119I TTCGAA 1 cut(s) 1025
Bsp1286I GDGCHC 2 cut(s) 707, 978
Bsp143I GATC 2 cut(s) 10, 871
BspACI CCGC 3 cut(s) 45, 117, 516
BspANI GGCC 2 cut(s) 29, 765
BspCNI CTCAG 4 cut(s) 217, 310, 418, 982
BspLI GGNNCC 4 cut(s) 58, 115, 397, 582
BspPI GGATC 1 cut(s) 866
BspQI GCTCTTC 1 cut(s) 156
BspT104I TTCGAA 1 cut(s) 1025
BspT107I GGYRCC 1 cut(s) 113
BsrBI CCGCTC 1 cut(s) 516
BsrDI GCAATG 1 cut(s) 267
BsrFI RCCGGY 1 cut(s) 821
BsrI ACTGG 3 cut(s) 205, 248, 418
BssAI RCCGGY 1 cut(s) 821
BssECI CCNNGG 4 cut(s) 193, 585, 709, 842
BssMI GATC 2 cut(s) 10, 871
BssNI GRCGYC 1 cut(s) 114
BssT1I CCWWGG 2 cut(s) 193, 842
Bst2UI CCWGG 6 cut(s) 70, 587, 711, 737, 773, 980
Bst4CI ACNGT 3 cut(s) 78, 551, 791
Bst6I CTCTTC 6 cut(s) 156, 228, 570, 607, 836, 1022
BstACI GRCGYC 1 cut(s) 114
BstAPI GCANNNNNTGC 1 cut(s) 948
BstBI TTCGAA 1 cut(s) 1025
BstC8I GCNNGC 2 cut(s) 126, 485
BstDEI CTNAG 4 cut(s) 225, 318, 426, 969
BstEII GGTNACC 1 cut(s) 72
BstF5I GGATG 1 cut(s) 595
BstH2I RGCGCY 1 cut(s) 117
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 2 cut(s) 13, 874
BstMBI GATC 2 cut(s) 10, 871
BstMWI GCNNNNNNNGC 4 cut(s) 54, 91, 125, 948
BstNI CCWGG 6 cut(s) 70, 587, 711, 737, 773, 980
BstPI GGTNACC 1 cut(s) 72
BstSCI CCNGG 6 cut(s) 68, 585, 709, 735, 771, 978
BstV1I GCAGC 1 cut(s) 970
BstV2I GAAGAC 1 cut(s) 476
BstXI CCANNNNNNTGG 3 cut(s) 200, 779, 935
Bsu36I CCTNAGG 1 cut(s) 969
BsuRI GGCC 2 cut(s) 29, 765
BtgZI GCGATG 1 cut(s) 63
BtsCI GGATG 1 cut(s) 595
BtsI GCAGTG 1 cut(s) 766
BtsIMutI CAGTG 4 cut(s) 198, 315, 411, 766
Cac8I GCNNGC 2 cut(s) 126, 485
CfoI GCGC 1 cut(s) 116
Cfr10I RCCGGY 1 cut(s) 821
Csp6I GTAC 3 cut(s) 40, 160, 301
CviAII CATG 3 cut(s) 91, 444, 700
CviQI GTAC 3 cut(s) 40, 160, 301
DdeI CTNAG 4 cut(s) 225, 318, 426, 969
DinI GGCGCC 1 cut(s) 115
DpnI GATC 2 cut(s) 12, 873
DpnII GATC 2 cut(s) 10, 871
DraIII CACNNNGTG 1 cut(s) 297
EaeI YGGCCR 2 cut(s) 27, 763
Eam1104I CTCTTC 6 cut(s) 156, 228, 570, 607, 836, 1022
EarI CTCTTC 6 cut(s) 156, 228, 570, 607, 836, 1022
Eco130I CCWWGG 2 cut(s) 193, 842
Eco81I CCTNAGG 1 cut(s) 969
Eco88I CYCGRG 1 cut(s) 530
Eco91I GGTNACC 1 cut(s) 72
EcoO65I GGTNACC 1 cut(s) 72
EcoRII CCWGG 6 cut(s) 68, 585, 709, 735, 771, 978
EcoT14I CCWWGG 2 cut(s) 193, 842
EgeI GGCGCC 1 cut(s) 115
EheI GGCGCC 1 cut(s) 115
ErhI CCWWGG 2 cut(s) 193, 842
FaeI CATG 3 cut(s) 94, 447, 703
FaiI YATR 6 cut(s) 92, 186, 445, 560, 701, 747
FatI CATG 3 cut(s) 90, 443, 699
Fnu4HI GCNGC 3 cut(s) 46, 117, 984
FokI GGATG 1 cut(s) 602
Fsp4HI GCNGC 3 cut(s) 46, 117, 984
FspBI CTAG 1 cut(s) 843
GlaI GCGC 1 cut(s) 115
GluI GCNGC 3 cut(s) 46, 117, 984
GsuI CTGGAG 2 cut(s) 719, 962
HaeII RGCGCY 1 cut(s) 117
HaeIII GGCC 2 cut(s) 29, 765
HapII CCGG 2 cut(s) 492, 822
HhaI GCGC 1 cut(s) 116
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 3 cut(s) 94, 447, 703
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HincII GTYRAC 2 cut(s) 595, 787
HindII GTYRAC 2 cut(s) 595, 787
HinfI GANTC 4 cut(s) 167, 472, 725, 998
HpaII CCGG 2 cut(s) 492, 822
HphI GGTGA 7 cut(s) 9, 66, 272, 380, 665, 751, 916
Hpy166II GTNNAC 4 cut(s) 40, 375, 595, 787
Hpy188I TCNGA 5 cut(s) 82, 166, 284, 1003, 1039
Hpy188III TCNNGA 3 cut(s) 131, 625, 875
Hpy8I GTNNAC 4 cut(s) 40, 375, 595, 787
Hpy99I CGWCG 1 cut(s) 82
HpyAV CCTTC 8 cut(s) 28, 95, 223, 232, 250, 535, 650, 949
HpyCH4III ACNGT 3 cut(s) 78, 551, 791
HpyCH4IV ACGT 1 cut(s) 993
HpyCH4V TGCA 2 cut(s) 206, 1043
HpyF10VI GCNNNNNNNGC 4 cut(s) 54, 91, 125, 948
HpyF3I CTNAG 4 cut(s) 225, 318, 426, 969
HpySE526I ACGT 1 cut(s) 993
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 3 cut(s) 94, 447, 703
HspAI GCGC 1 cut(s) 114
KasI GGCGCC 1 cut(s) 113
Kzo9I GATC 2 cut(s) 10, 871
LguI GCTCTTC 1 cut(s) 156
LmnI GCTCC 8 cut(s) 4, 62, 82, 99, 401, 586, 710, 981
Lsp1109I GCAGC 1 cut(s) 970
LweI GCATC 2 cut(s) 253, 1030
MaeI CTAG 1 cut(s) 843
MaeII ACGT 1 cut(s) 993
MaeIII GTNAC 5 cut(s) 72, 104, 289, 520, 739
MalI GATC 2 cut(s) 12, 873
MbiI CCGCTC 1 cut(s) 516
MboI GATC 2 cut(s) 10, 871
MhlI GDGCHC 2 cut(s) 707, 978
MlsI TGGCCA 1 cut(s) 29
MluCI AATT 1 cut(s) 213
MluNI TGGCCA 1 cut(s) 29
Mly113I GGCGCC 1 cut(s) 114
MlyI GAGTC 2 cut(s) 466, 719
MmeI TCCRAC 4 cut(s) 60, 190, 423, 666
Mox20I TGGCCA 1 cut(s) 29
MroXI GAANNNNTTC 1 cut(s) 1016
MscI TGGCCA 1 cut(s) 29
MseI TTAA 3 cut(s) 252, 600, 816
MslI CAYNNNNRTG 2 cut(s) 552, 1063
Msp20I TGGCCA 1 cut(s) 29
MspI CCGG 2 cut(s) 492, 822
MspR9I CCNGG 6 cut(s) 70, 587, 711, 737, 773, 980
MvaI CCWGG 6 cut(s) 70, 587, 711, 737, 773, 980
MwoI GCNNNNNNNGC 4 cut(s) 54, 91, 125, 948
NarI GGCGCC 1 cut(s) 114
NdeII GATC 2 cut(s) 10, 871
NlaIII CATG 3 cut(s) 94, 447, 703
NlaIV GGNNCC 4 cut(s) 58, 115, 397, 582
NmeAIII GCCGAG 1 cut(s) 707
NmuCI GTSAC 3 cut(s) 72, 289, 739
NspV TTCGAA 1 cut(s) 1025
OliI CACNNNNGTG 1 cut(s) 1063
PciSI GCTCTTC 1 cut(s) 156
PdmI GAANNNNTTC 1 cut(s) 1016
PfeI GAWTC 2 cut(s) 167, 998
PkrI GCNGC 3 cut(s) 47, 118, 985
PleI GAGTC 2 cut(s) 466, 719
PluTI GGCGCC 1 cut(s) 117
PpsI GAGTC 2 cut(s) 466, 719
Psp6I CCWGG 6 cut(s) 68, 585, 709, 735, 771, 978
PspEI GGTNACC 1 cut(s) 72
PspGI CCWGG 6 cut(s) 68, 585, 709, 735, 771, 978
PspN4I GGNNCC 4 cut(s) 58, 115, 397, 582
RsaI GTAC 3 cut(s) 41, 161, 302
RsaNI GTAC 3 cut(s) 40, 160, 301
RseI CAYNNNNRTG 2 cut(s) 552, 1063
SapI GCTCTTC 1 cut(s) 156
SaqAI TTAA 3 cut(s) 252, 600, 816
SatI GCNGC 3 cut(s) 46, 117, 984
Sau3AI GATC 2 cut(s) 10, 871
ScaI AGTACT 1 cut(s) 161
SchI GAGTC 2 cut(s) 466, 719
ScrFI CCNGG 6 cut(s) 70, 587, 711, 737, 773, 980
SduI GDGCHC 2 cut(s) 707, 978
SfaNI GCATC 2 cut(s) 253, 1030
SfoI GGCGCC 1 cut(s) 115
SfuI TTCGAA 1 cut(s) 1025
SmiMI CAYNNNNRTG 2 cut(s) 552, 1063
Sse9I AATT 1 cut(s) 213
SsiI CCGC 3 cut(s) 45, 117, 516
SspDI GGCGCC 1 cut(s) 113
SspMI CTAG 1 cut(s) 843
StyD4I CCNGG 6 cut(s) 68, 585, 709, 735, 771, 978
StyI CCWWGG 2 cut(s) 193, 842
TaaI ACNGT 3 cut(s) 78, 551, 791
TaiI ACGT 1 cut(s) 996
TaqI TCGA 1 cut(s) 1025
TasI AATT 1 cut(s) 213
TatI WGTACW 1 cut(s) 159
TauI GCSGC 2 cut(s) 48, 119
TfiI GAWTC 2 cut(s) 167, 998
Tru1I TTAA 3 cut(s) 252, 600, 816
Tru9I TTAA 3 cut(s) 252, 600, 816
TscAI CASTG 4 cut(s) 205, 322, 418, 766
TseFI GTSAC 3 cut(s) 72, 289, 739
TseI GCWGC 1 cut(s) 983
Tsp45I GTSAC 3 cut(s) 72, 289, 739
TspDTI ATGAA 1 cut(s) 823
TspGWI ACGGA 3 cut(s) 86, 705, 1010
TspRI CASTG 4 cut(s) 205, 322, 418, 766
XcmI CCANNNNNNNNNTGG 1 cut(s) 127
XmaJI CCTAGG 1 cut(s) 842
XmnI GAANNNNTTC 1 cut(s) 1016
XspI CTAG 1 cut(s) 843
ZrmI AGTACT 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.