pycom03g08290

Glutelin type-A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0001439
Physical Location & Seq
Forward (+)
6688382 .. 6689890
1509 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g08290.1

Sequence Viewer

Length: 1041 bp
ATGGAGCTTGATCTTTCACCCAAGTTGGCCAAGAAGGTGTACGGCGGCGATGGCGGGTCCTACTTTGCCTGGTCCCCGTCGGAGCTTCCCATGCTCCGTGAAGGTAACATCGGCGCCGCCAAGCTCGCTCTGGAGAAGGACGGGTTCGCTCTCCCCAAGTACTCTGACTCCAACCAGGTCGCCTATGTCCTCCAAGGTACTGGTGTAGTCGGAATTGTCCTCCCGGAGAAGGAAGAGAAGGTTCTTCCAGTTAAGAAGGGTGATGCGATTGCCCTCCCTTTCGGAGTTGTGACTTGGTGGTACAACAAGGAGGACACCGAGTTCGTTGTTCTTTTCTTGGGCGACACTTCAAAAGCTCACAAGAGGGGTGAGTTTACCGACTTCTATCTCAACGGCTCCAACGCCATTTTCACCGGCTTCTCAACCGAGTTTGTTGGTCGGGCATGGGATTTGGAGGAGAGTGTTGTGAAGACTCTCGTTGGCAAGCAGTCTGGCAAGGGCATTGTCAAGCTCAGCGGAGCTAAGTTGCCCGAGCCAAAGAAGGAACACCGCGATGGCATGACGTTGAACTGCGAAGAGGCCCCCTTGGATGTTGACATTAAGGATGGTGGAAGAGTTGTGGTCTTGAATACCAAAAACCTTCCTTTGGTCGGTGAGGTTGGGCTCGGTGCTGACCTCGTGAGGTTGGACGGGAGTGCCATGTGCTCCCCTGGATTTTCTTGTGACTCGGCTCTGCAGGTGACTTATATTGTTAGGGGCAGCGGCCGTGTCCAGGTTGTTGGTGTCGACGGTAAGAGGGTGTTGGAAACTACCATCAAAGCCGGTAATCTCTTCATCGTTCCTCGGTTCTTTGTTGTGTCAAAGATTGCTCGATCCGGAGGGCTTGGAATGGTTTTCTATCATCACTACGCCCAACCCGATATTCACCCACTTGGCTGGAAGCATTGGATGCTGGAAGGCTCTATCTCCGCAGGTGCTCCAGGCTGCGTTCAATGTGGATTCGGAAACAGAGAAACTCTTCCGTTCGAAGAGGACTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

347

Amino Acids

37.43

Weight (kDa)

5.94

Isoelectric Point (pI)

34.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 11 - 156 4.1e-24 Cupin
Cupin_1 PF00190 197 - 292 7e-15 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 727, 962
Acc36I ACCTGC 2 cut(s) 727, 962
AccB1I GGYRCC 1 cut(s) 113
AccI GTMKAC 1 cut(s) 786
AccII CGCG 1 cut(s) 552
AccIII TCCGGA 1 cut(s) 875
AciI CCGC 7 cut(s) 45, 54, 117, 516, 550, 762, 969
AclWI GGATC 1 cut(s) 867
AcoI YGGCCR 2 cut(s) 27, 763
AcyI GRCGYC 1 cut(s) 114
AfaI GTAC 4 cut(s) 41, 161, 199, 302
AfiI CCNNNNNNNGG 4 cut(s) 229, 646, 650, 772
AgsI TTSAA 4 cut(s) 351, 568, 628, 992
AjnI CCWGG 5 cut(s) 68, 174, 709, 771, 979
AjuI GAANNNNNNNTTGG 4 cut(s) 392, 424, 906, 938
AloI GAACNNNNNNTCC 2 cut(s) 305, 337
AluBI AGCT 6 cut(s) 7, 85, 124, 356, 511, 521
AluI AGCT 6 cut(s) 7, 85, 124, 356, 511, 521
Alw21I GWGCWC 2 cut(s) 707, 979
AlwI GGATC 1 cut(s) 867
Ama87I CYCGRG 1 cut(s) 530
Aor13HI TCCGGA 1 cut(s) 875
AoxI GGCC 3 cut(s) 27, 579, 763
ApeKI GCWGC 2 cut(s) 759, 984
ArsI GACNNNNNNTTYG 2 cut(s) 305, 337
Asp700I GAANNNNTTC 1 cut(s) 1017
AspLEI GCGC 1 cut(s) 116
AspS9I GGNCC 3 cut(s) 57, 72, 580
AsuC2I CCSGG 1 cut(s) 224
AsuHPI GGTGA 7 cut(s) 9, 272, 380, 403, 665, 751, 917
AsuII TTCGAA 1 cut(s) 1026
AvaI CYCGRG 1 cut(s) 530
AvaII GGWCC 2 cut(s) 57, 72
BaeI ACNNNNGTAYC 2 cut(s) 189, 222
BalI TGGCCA 1 cut(s) 29
BanI GGYRCC 1 cut(s) 113
BanII GRGCYC 1 cut(s) 666
BauI CACGAG 1 cut(s) 677
BbsI GAAGAC 1 cut(s) 476
Bbv12I GWGCWC 2 cut(s) 707, 979
BbvI GCAGC 2 cut(s) 771, 971
BccI CCATC 4 cut(s) 44, 548, 599, 821
BceAI ACGGC 3 cut(s) 58, 409, 750
BciT130I CCWGG 5 cut(s) 70, 176, 711, 773, 981
BcnI CCSGG 1 cut(s) 224
BfmI CTRYAG 1 cut(s) 734
BfoI RGCGCY 1 cut(s) 117
BfuAI ACCTGC 2 cut(s) 727, 962
BisI GCNGC 5 cut(s) 46, 117, 760, 763, 985
BlpI GCTNAGC 1 cut(s) 512
BlsI GCNGC 5 cut(s) 47, 118, 761, 764, 986
BmcAI AGTACT 1 cut(s) 161
Bme1390I CCNGG 6 cut(s) 70, 176, 224, 711, 773, 981
Bme18I GGWCC 2 cut(s) 57, 72
BmeT110I CYCGRG 1 cut(s) 530
BmgT120I GGNCC 3 cut(s) 57, 72, 580
BmiI GGNNCC 5 cut(s) 58, 74, 115, 397, 582
BmrFI CCNGG 6 cut(s) 70, 176, 224, 711, 773, 981
BmsI GCATC 2 cut(s) 253, 939
BpiI GAAGAC 1 cut(s) 476
BpmI CTGGAG 2 cut(s) 152, 963
Bpu1102I GCTNAGC 1 cut(s) 512
Bpu14I TTCGAA 1 cut(s) 1026
BpuMI CCSGG 1 cut(s) 224
BsaHI GRCGYC 1 cut(s) 114
BsaJI CCNNGG 4 cut(s) 193, 585, 709, 842
BsaWI WCCGGW 1 cut(s) 875
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 4 cut(s) 229, 646, 650, 772
Bse118I RCCGGY 2 cut(s) 413, 821
Bse1I ACTGG 2 cut(s) 205, 248
BseAI TCCGGA 1 cut(s) 875
BseBI CCWGG 5 cut(s) 70, 176, 711, 773, 981
BseDI CCNNGG 4 cut(s) 193, 585, 709, 842
BseGI GGATG 3 cut(s) 595, 610, 954
BseLI CCNNNNNNNGG 4 cut(s) 229, 646, 650, 772
BseMII CTCAG 1 cut(s) 526
BseNI ACTGG 2 cut(s) 205, 248
BseRI GAGGAG 1 cut(s) 470
BseX3I CGGCCG 1 cut(s) 763
BseXI GCAGC 2 cut(s) 771, 971
Bsh1236I CGCG 1 cut(s) 552
Bsh1285I CGRYCG 1 cut(s) 766
BshFI GGCC 3 cut(s) 29, 581, 765
BshNI GGYRCC 1 cut(s) 113
BsiEI CGRYCG 1 cut(s) 766
BsiHKAI GWGCWC 2 cut(s) 707, 979
BsiHKCI CYCGRG 1 cut(s) 530
BsiSI CCGG 4 cut(s) 224, 414, 822, 876
BslFI GGGAC 1 cut(s) 58
BslI CCNNNNNNNGG 4 cut(s) 229, 646, 650, 772
BsmFI GGGAC 1 cut(s) 58
BsnI GGCC 3 cut(s) 29, 581, 765
BsoBI CYCGRG 1 cut(s) 530
Bsp119I TTCGAA 1 cut(s) 1026
Bsp1286I GDGCHC 3 cut(s) 666, 707, 979
Bsp13I TCCGGA 1 cut(s) 875
Bsp143I GATC 2 cut(s) 10, 872
Bsp1720I GCTNAGC 1 cut(s) 512
BspACI CCGC 7 cut(s) 45, 54, 117, 516, 550, 762, 969
BspANI GGCC 3 cut(s) 29, 581, 765
BspCNI CTCAG 1 cut(s) 525
BspEI TCCGGA 1 cut(s) 875
BspFNI CGCG 1 cut(s) 552
BspLI GGNNCC 5 cut(s) 58, 74, 115, 397, 582
BspMAI CTGCAG 1 cut(s) 738
BspMI ACCTGC 2 cut(s) 727, 962
BspPI GGATC 1 cut(s) 867
BspT104I TTCGAA 1 cut(s) 1026
BspT107I GGYRCC 1 cut(s) 113
BsrFI RCCGGY 2 cut(s) 413, 821
BsrI ACTGG 2 cut(s) 205, 248
BssAI RCCGGY 2 cut(s) 413, 821
BssECI CCNNGG 4 cut(s) 193, 585, 709, 842
BssMI GATC 2 cut(s) 10, 872
BssNI GRCGYC 1 cut(s) 114
BssSI CACGAG 1 cut(s) 677
BssT1I CCWWGG 2 cut(s) 193, 585
Bst2BI CACGAG 1 cut(s) 677
Bst2UI CCWGG 5 cut(s) 70, 176, 711, 773, 981
Bst4CI ACNGT 1 cut(s) 791
Bst6I CTCTTC 6 cut(s) 228, 570, 607, 836, 1023, 1023
BstACI GRCGYC 1 cut(s) 114
BstAPI GCANNNNNTGC 1 cut(s) 949
BstBI TTCGAA 1 cut(s) 1026
BstC8I GCNNGC 2 cut(s) 126, 485
BstDEI CTNAG 2 cut(s) 512, 522
BstF5I GGATG 3 cut(s) 595, 610, 954
BstFNI CGCG 1 cut(s) 552
BstH2I RGCGCY 1 cut(s) 117
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 2 cut(s) 13, 875
BstMBI GATC 2 cut(s) 10, 872
BstMCI CGRYCG 1 cut(s) 766
BstMWI GCNNNNNNNGC 4 cut(s) 51, 91, 125, 949
BstNI CCWGG 5 cut(s) 70, 176, 711, 773, 981
BstSCI CCNGG 6 cut(s) 68, 174, 222, 709, 771, 979
BstSFI CTRYAG 1 cut(s) 734
BstUI CGCG 1 cut(s) 552
BstV1I GCAGC 2 cut(s) 771, 971
BstV2I GAAGAC 1 cut(s) 476
BstXI CCANNNNNNTGG 3 cut(s) 200, 779, 936
BstZI CGGCCG 1 cut(s) 763
BsuRI GGCC 3 cut(s) 29, 581, 765
BtgZI GCGATG 2 cut(s) 63, 567
BtsCI GGATG 3 cut(s) 595, 610, 954
BveI ACCTGC 2 cut(s) 727, 962
Cac8I GCNNGC 2 cut(s) 126, 485
CfoI GCGC 1 cut(s) 116
Cfr10I RCCGGY 2 cut(s) 413, 821
Cfr13I GGNCC 3 cut(s) 57, 72, 580
CsiI ACCWGGT 1 cut(s) 174
Csp6I GTAC 4 cut(s) 40, 160, 198, 301
CviAII CATG 4 cut(s) 91, 444, 559, 700
CviQI GTAC 4 cut(s) 40, 160, 198, 301
DdeI CTNAG 2 cut(s) 512, 522
DinI GGCGCC 1 cut(s) 115
DpnI GATC 2 cut(s) 12, 874
DpnII GATC 2 cut(s) 10, 872
EaeI YGGCCR 2 cut(s) 27, 763
EagI CGGCCG 1 cut(s) 763
Eam1104I CTCTTC 6 cut(s) 228, 570, 607, 836, 1023, 1023
EarI CTCTTC 6 cut(s) 228, 570, 607, 836, 1023, 1023
EclXI CGGCCG 1 cut(s) 763
Eco130I CCWWGG 2 cut(s) 193, 585
Eco24I GRGCYC 1 cut(s) 666
Eco47I GGWCC 2 cut(s) 57, 72
Eco52I CGGCCG 1 cut(s) 763
Eco88I CYCGRG 1 cut(s) 530
EcoO109I RGGNCCY 2 cut(s) 57, 580
EcoRII CCWGG 5 cut(s) 68, 174, 709, 771, 979
EcoT14I CCWWGG 2 cut(s) 193, 585
EcoT38I GRGCYC 1 cut(s) 666
EgeI GGCGCC 1 cut(s) 115
EheI GGCGCC 1 cut(s) 115
ErhI CCWWGG 2 cut(s) 193, 585
FaeI CATG 4 cut(s) 94, 447, 562, 703
FaiI YATR 6 cut(s) 92, 186, 445, 560, 701, 747
FaqI GGGAC 1 cut(s) 58
FatI CATG 4 cut(s) 90, 443, 558, 699
FauI CCCGC 1 cut(s) 47
FblI GTMKAC 1 cut(s) 786
Fnu4HI GCNGC 5 cut(s) 46, 117, 760, 763, 985
FokI GGATG 3 cut(s) 602, 617, 961
FriOI GRGCYC 1 cut(s) 666
Fsp4HI GCNGC 5 cut(s) 46, 117, 760, 763, 985
GlaI GCGC 1 cut(s) 115
GluI GCNGC 5 cut(s) 46, 117, 760, 763, 985
GsuI CTGGAG 2 cut(s) 152, 963
HaeII RGCGCY 1 cut(s) 117
HaeIII GGCC 3 cut(s) 29, 581, 765
HapII CCGG 4 cut(s) 224, 414, 822, 876
HhaI GCGC 1 cut(s) 116
Hin1I GRCGYC 1 cut(s) 114
Hin1II CATG 4 cut(s) 94, 447, 562, 703
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HincII GTYRAC 2 cut(s) 595, 787
HindII GTYRAC 2 cut(s) 595, 787
HinfI GANTC 4 cut(s) 167, 472, 725, 999
HpaII CCGG 4 cut(s) 224, 414, 822, 876
HphI GGTGA 7 cut(s) 9, 272, 380, 403, 665, 751, 917
Hpy166II GTNNAC 4 cut(s) 40, 375, 595, 787
Hpy188I TCNGA 5 cut(s) 82, 166, 212, 284, 1004
Hpy188III TCNNGA 4 cut(s) 131, 625, 679, 876
Hpy8I GTNNAC 4 cut(s) 40, 375, 595, 787
Hpy99I CGWCG 2 cut(s) 82, 791
HpyAV CCTTC 9 cut(s) 28, 95, 130, 223, 232, 250, 535, 650, 950
HpyCH4III ACNGT 1 cut(s) 791
HpyCH4IV ACGT 1 cut(s) 563
HpyCH4V TGCA 1 cut(s) 736
HpyF10VI GCNNNNNNNGC 4 cut(s) 51, 91, 125, 949
HpyF3I CTNAG 2 cut(s) 512, 522
HpySE526I ACGT 1 cut(s) 563
Hsp92I GRCGYC 1 cut(s) 114
Hsp92II CATG 4 cut(s) 94, 447, 562, 703
HspAI GCGC 1 cut(s) 114
KasI GGCGCC 1 cut(s) 113
Kpn2I TCCGGA 1 cut(s) 875
Kzo9I GATC 2 cut(s) 10, 872
LmnI GCTCC 7 cut(s) 4, 82, 99, 401, 518, 710, 982
Lsp1109I GCAGC 2 cut(s) 771, 971
LweI GCATC 2 cut(s) 253, 939
MabI ACCWGGT 1 cut(s) 174
MaeII ACGT 1 cut(s) 563
MaeIII GTNAC 4 cut(s) 104, 289, 722, 739
MalI GATC 2 cut(s) 12, 874
MboI GATC 2 cut(s) 10, 872
MboII GAAGA 8 cut(s) 236, 245, 481, 587, 624, 823, 1010, 1040
MhlI GDGCHC 3 cut(s) 666, 707, 979
MlsI TGGCCA 1 cut(s) 29
MluCI AATT 1 cut(s) 213
MluNI TGGCCA 1 cut(s) 29
Mly113I GGCGCC 1 cut(s) 114
MlyI GAGTC 3 cut(s) 161, 466, 719
MmeI TCCRAC 6 cut(s) 60, 190, 195, 423, 666, 783
Mox20I TGGCCA 1 cut(s) 29
MroI TCCGGA 1 cut(s) 875
MroXI GAANNNNTTC 1 cut(s) 1017
MscI TGGCCA 1 cut(s) 29
MseI TTAA 2 cut(s) 252, 600
MslI CAYNNNNRTG 1 cut(s) 552
Msp20I TGGCCA 1 cut(s) 29
MspA1I CMGCKG 2 cut(s) 516, 762
MspI CCGG 4 cut(s) 224, 414, 822, 876
MspR9I CCNGG 6 cut(s) 70, 176, 224, 711, 773, 981
MvaI CCWGG 5 cut(s) 70, 176, 711, 773, 981
MvnI CGCG 1 cut(s) 552
MwoI GCNNNNNNNGC 4 cut(s) 51, 91, 125, 949
NarI GGCGCC 1 cut(s) 114
NciI CCSGG 1 cut(s) 224
NdeII GATC 2 cut(s) 10, 872
NlaIII CATG 4 cut(s) 94, 447, 562, 703
NlaIV GGNNCC 5 cut(s) 58, 74, 115, 397, 582
NmeAIII GCCGAG 1 cut(s) 707
NmuCI GTSAC 3 cut(s) 289, 722, 739
NspV TTCGAA 1 cut(s) 1026
PaqCI CACCTGC 2 cut(s) 727, 962
PcsI WCGNNNNNNNCGW 1 cut(s) 915
PdmI GAANNNNTTC 1 cut(s) 1017
PfeI GAWTC 1 cut(s) 999
PfoI TCCNGGA 1 cut(s) 222
PkrI GCNGC 5 cut(s) 47, 118, 761, 764, 986
PleI GAGTC 3 cut(s) 161, 466, 719
PluTI GGCGCC 1 cut(s) 117
PpsI GAGTC 3 cut(s) 161, 466, 719
PpuMI RGGWCCY 1 cut(s) 57
Psp5II RGGWCCY 1 cut(s) 57
Psp6I CCWGG 5 cut(s) 68, 174, 709, 771, 979
PspGI CCWGG 5 cut(s) 68, 174, 709, 771, 979
PspN4I GGNNCC 5 cut(s) 58, 74, 115, 397, 582
PspPI GGNCC 3 cut(s) 57, 72, 580
PspPPI RGGWCCY 1 cut(s) 57
PstI CTGCAG 1 cut(s) 738
RsaI GTAC 4 cut(s) 41, 161, 199, 302
RsaNI GTAC 4 cut(s) 40, 160, 198, 301
RseI CAYNNNNRTG 1 cut(s) 552
SalI GTCGAC 1 cut(s) 785
SaqAI TTAA 2 cut(s) 252, 600
SatI GCNGC 5 cut(s) 46, 117, 760, 763, 985
Sau3AI GATC 2 cut(s) 10, 872
Sau96I GGNCC 3 cut(s) 57, 72, 580
ScaI AGTACT 1 cut(s) 161
SchI GAGTC 3 cut(s) 161, 466, 719
ScrFI CCNGG 6 cut(s) 70, 176, 224, 711, 773, 981
SduI GDGCHC 3 cut(s) 666, 707, 979
SexAI ACCWGGT 1 cut(s) 174
SfaNI GCATC 2 cut(s) 253, 939
SfcI CTRYAG 1 cut(s) 734
SfoI GGCGCC 1 cut(s) 115
SfuI TTCGAA 1 cut(s) 1026
SinI GGWCC 2 cut(s) 57, 72
SmiMI CAYNNNNRTG 1 cut(s) 552
Sse9I AATT 1 cut(s) 213
SsiI CCGC 7 cut(s) 45, 54, 117, 516, 550, 762, 969
SspDI GGCGCC 1 cut(s) 113
StyD4I CCNGG 6 cut(s) 68, 174, 222, 709, 771, 979
StyI CCWWGG 2 cut(s) 193, 585
TaaI ACNGT 1 cut(s) 791
TaiI ACGT 1 cut(s) 566
TaqI TCGA 3 cut(s) 786, 871, 1026
TasI AATT 1 cut(s) 213
TatI WGTACW 1 cut(s) 159
TauI GCSGC 3 cut(s) 48, 119, 765
TfiI GAWTC 1 cut(s) 999
Tru1I TTAA 2 cut(s) 252, 600
Tru9I TTAA 2 cut(s) 252, 600
TseFI GTSAC 3 cut(s) 289, 722, 739
TseI GCWGC 2 cut(s) 759, 984
Tsp45I GTSAC 3 cut(s) 289, 722, 739
TspDTI ATGAA 1 cut(s) 823
TspGWI ACGGA 2 cut(s) 86, 1011
VpaK11BI GGWCC 2 cut(s) 57, 72
XcmI CCANNNNNNNNNTGG 1 cut(s) 127
XmiI GTMKAC 1 cut(s) 786
XmnI GAANNNNTTC 1 cut(s) 1017
ZrmI AGTACT 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.