Rh5DG439700

Glutelin type-A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001439
Physical Location & Seq
Reverse (-)
70485335 .. 70488128
2794 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG439700.1

Sequence Viewer

Length: 918 bp
ATGGATATTGATCTTGCACCAAAGTTGGCCAAGAAGGTTTATGGAGGAGATGGAGGTTCTTACTTTGCTTGGTGCCCATCGGAGCTTCCCATGCTCCGTGAAGGTAACATCGGCGCTGCCAAGCTCGCCCTTGAGAAGAACGGCTTTGCTCTCCCCAATTACTCTGATTCTGCCAGAGTTGCTTATATCCTCCAAGGAAGTGGTGTTGTTGGAATTGTCCTCCCGGAGAAGGAAGAGAAGGTTCTTCCAGTGAAGAAGGGTGATGCCATTGCCCTCCCTTTCGGAGTTATCACTTGGTGGCACAACAAGGAGGACACTGAGTTTGTAGTTCTTTTCTTGGGTGATACCAAAACAGCTCACAAAAGGGGTGAGTTCACCAACTTCTATCTCACTGGTACTAATGGCATTTTCACTGGTTTCTCAACTGAGTTTGTCGGCCGAGCATGGGATTTGGAGGAGAGTGTTGTGAAGACTCTTGTTGGCAAGCAAAGCGGTCAGGGCATTGTTAAGTTGGGGGCATCTAATTTGCCTGAGGCAAAGAACGAGCATAGAGATGGGATGACATTGAATTGCGAAGAAGCTCCCTTAGATGTTGATATCAAGGGTGGTGGAAGAGTTGTTGTTTTGAATACTAAGAACCTTCCTTTGGTCGGAGAGGTTGGGCTTGGTGCTGATCTGGTTAGGTTGGACGGAAGTGCCATGTGCTCCCCTGGGTTTTCTTGTGACTCTGCATTGCAGGTGACTTACATTGTTAGAGGCAGTGGCCGTGCCCAAGTTGTTGGGGTTGATGGGAAGAGGGTCTTGGAAACAACCGTCAAAGCTGGTAACTTGTTCATTGTTCCTAGATTCTTTATTGTTTCAAAAATTGCTGATCCAGAAGGCTTGGAATGGTTCTCCATCATCACTACTCCCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

305

Amino Acids

32.77

Weight (kDa)

5.77

Isoelectric Point (pI)

26.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 11 - 156 3.5e-23 Cupin
Cupin_1 PF00190 198 - 302 1.1e-15 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 727
Acc36I ACCTGC 1 cut(s) 727
AccB1I GGYRCC 1 cut(s) 72
AciI CCGC 1 cut(s) 492
AclWI GGATC 1 cut(s) 866
AcoI YGGCCR 3 cut(s) 27, 436, 763
AdeI CACNNNGTG 1 cut(s) 297
AfaI GTAC 1 cut(s) 397
AfiI CCNNNNNNNGG 4 cut(s) 229, 445, 646, 650
AgsI TTSAA 3 cut(s) 568, 628, 861
AjnI CCWGG 1 cut(s) 709
AjuI GAANNNNNNNTTGG 4 cut(s) 629, 661, 785, 817
AluBI AGCT 5 cut(s) 85, 124, 356, 581, 821
AluI AGCT 5 cut(s) 85, 124, 356, 581, 821
Alw21I GWGCWC 1 cut(s) 707
AlwI GGATC 1 cut(s) 866
AoxI GGCC 3 cut(s) 27, 436, 763
ApeKI GCWGC 1 cut(s) 116
ArsI GACNNNNNNTTYG 2 cut(s) 305, 337
AspLEI GCGC 1 cut(s) 116
AsuC2I CCSGG 1 cut(s) 224
AsuHPI GGTGA 5 cut(s) 272, 353, 367, 380, 751
AxyI CCTNAGG 1 cut(s) 531
BaeGI GKGCMC 2 cut(s) 77, 772
BalI TGGCCA 1 cut(s) 29
BanI GGYRCC 1 cut(s) 72
BbsI GAAGAC 1 cut(s) 476
Bbv12I GWGCWC 1 cut(s) 707
BbvI GCAGC 1 cut(s) 103
BccI CCATC 5 cut(s) 44, 85, 548, 782, 905
BceAI ACGGC 2 cut(s) 157, 750
BciT130I CCWGG 1 cut(s) 711
BcnI CCSGG 1 cut(s) 224
BfaI CTAG 1 cut(s) 843
BfoI RGCGCY 1 cut(s) 117
BfuAI ACCTGC 1 cut(s) 727
BisI GCNGC 1 cut(s) 117
BlsI GCNGC 1 cut(s) 118
Bme1390I CCNGG 2 cut(s) 224, 711
BmiI GGNNCC 1 cut(s) 74
BmrFI CCNGG 2 cut(s) 224, 711
BmsI GCATC 2 cut(s) 253, 527
BpiI GAAGAC 1 cut(s) 476
BpuEI CTTGAG 1 cut(s) 152
BpuMI CCSGG 1 cut(s) 224
BsaBI GATNNNNATC 1 cut(s) 9
BsaJI CCNNGG 3 cut(s) 193, 709, 710
Bsc4I CCNNNNNNNGG 4 cut(s) 229, 445, 646, 650
Bse1I ACTGG 3 cut(s) 248, 397, 418
Bse21I CCTNAGG 1 cut(s) 531
Bse3DI GCAATG 2 cut(s) 267, 731
Bse8I GATNNNNATC 1 cut(s) 9
BseBI CCWGG 1 cut(s) 711
BseDI CCNNGG 3 cut(s) 193, 709, 710
BseGI GGATG 1 cut(s) 564
BseJI GATNNNNATC 1 cut(s) 9
BseLI CCNNNNNNNGG 4 cut(s) 229, 445, 646, 650
BseMI GCAATG 2 cut(s) 267, 731
BseMII CTCAG 3 cut(s) 309, 417, 522
BseNI ACTGG 3 cut(s) 248, 397, 418
BseRI GAGGAG 2 cut(s) 60, 470
BseSI GKGCMC 2 cut(s) 77, 772
BseX3I CGGCCG 1 cut(s) 436
BseXI GCAGC 1 cut(s) 103
Bsh1285I CGRYCG 1 cut(s) 439
BshFI GGCC 3 cut(s) 29, 438, 765
BshNI GGYRCC 1 cut(s) 72
BsiEI CGRYCG 1 cut(s) 439
BsiHKAI GWGCWC 1 cut(s) 707
BsiSI CCGG 1 cut(s) 224
BslI CCNNNNNNNGG 4 cut(s) 229, 445, 646, 650
BsnI GGCC 3 cut(s) 29, 438, 765
Bsp1286I GDGCHC 3 cut(s) 77, 707, 772
Bsp143I GATC 3 cut(s) 10, 673, 871
BspACI CCGC 1 cut(s) 492
BspANI GGCC 3 cut(s) 29, 438, 765
BspCNI CTCAG 3 cut(s) 310, 418, 523
BspLI GGNNCC 1 cut(s) 74
BspMI ACCTGC 1 cut(s) 727
BspPI GGATC 1 cut(s) 866
BspT107I GGYRCC 1 cut(s) 72
BsrDI GCAATG 2 cut(s) 267, 731
BsrI ACTGG 3 cut(s) 248, 397, 418
BssECI CCNNGG 3 cut(s) 193, 709, 710
BssMI GATC 3 cut(s) 10, 673, 871
BssT1I CCWWGG 1 cut(s) 193
Bst2UI CCWGG 1 cut(s) 711
Bst4CI ACNGT 1 cut(s) 814
Bst6I CTCTTC 3 cut(s) 228, 607, 788
BstC8I GCNNGC 2 cut(s) 126, 485
BstDEI CTNAG 5 cut(s) 318, 426, 531, 586, 633
BstF5I GGATG 1 cut(s) 564
BstH2I RGCGCY 1 cut(s) 117
BstHHI GCGC 1 cut(s) 116
BstKTI GATC 3 cut(s) 13, 676, 874
BstMBI GATC 3 cut(s) 10, 673, 871
BstMCI CGRYCG 1 cut(s) 439
BstMWI GCNNNNNNNGC 5 cut(s) 91, 125, 179, 489, 498
BstNI CCWGG 1 cut(s) 711
BstSCI CCNGG 2 cut(s) 222, 709
BstSLI GKGCMC 2 cut(s) 77, 772
BstV1I GCAGC 1 cut(s) 103
BstV2I GAAGAC 1 cut(s) 476
BstXI CCANNNNNNTGG 2 cut(s) 200, 779
BstZI CGGCCG 1 cut(s) 436
Bsu36I CCTNAGG 1 cut(s) 531
BsuRI GGCC 3 cut(s) 29, 438, 765
BtsCI GGATG 1 cut(s) 564
BtsI GCAGTG 1 cut(s) 766
BtsIMutI CAGTG 5 cut(s) 255, 315, 390, 411, 766
BveI ACCTGC 1 cut(s) 727
Cac8I GCNNGC 2 cut(s) 126, 485
CfoI GCGC 1 cut(s) 116
Csp6I GTAC 1 cut(s) 396
CspCI CAANNNNNGTGG 2 cut(s) 589, 624
CviAII CATG 3 cut(s) 91, 444, 700
CviQI GTAC 1 cut(s) 396
DdeI CTNAG 5 cut(s) 318, 426, 531, 586, 633
DpnI GATC 3 cut(s) 12, 675, 873
DpnII GATC 3 cut(s) 10, 673, 871
DraIII CACNNNGTG 1 cut(s) 297
EaeI YGGCCR 3 cut(s) 27, 436, 763
EagI CGGCCG 1 cut(s) 436
Eam1104I CTCTTC 3 cut(s) 228, 607, 788
EarI CTCTTC 3 cut(s) 228, 607, 788
EclXI CGGCCG 1 cut(s) 436
Eco130I CCWWGG 1 cut(s) 193
Eco32I GATATC 1 cut(s) 598
Eco52I CGGCCG 1 cut(s) 436
Eco81I CCTNAGG 1 cut(s) 531
EcoRII CCWGG 1 cut(s) 709
EcoRV GATATC 1 cut(s) 598
EcoT14I CCWWGG 1 cut(s) 193
ErhI CCWWGG 1 cut(s) 193
FaeI CATG 3 cut(s) 94, 447, 703
FaiI YATR 6 cut(s) 42, 92, 186, 445, 549, 701
FalI AAGNNNNNCTT 4 cut(s) 128, 160, 785, 817
FatI CATG 3 cut(s) 90, 443, 699
Fnu4HI GCNGC 1 cut(s) 117
FokI GGATG 1 cut(s) 571
Fsp4HI GCNGC 1 cut(s) 117
FspBI CTAG 1 cut(s) 843
GlaI GCGC 1 cut(s) 115
GluI GCNGC 1 cut(s) 117
HaeII RGCGCY 1 cut(s) 117
HaeIII GGCC 3 cut(s) 29, 438, 765
HapII CCGG 1 cut(s) 224
HhaI GCGC 1 cut(s) 116
Hin1II CATG 3 cut(s) 94, 447, 703
Hin6I GCGC 1 cut(s) 114
HinP1I GCGC 1 cut(s) 114
HinfI GANTC 4 cut(s) 167, 472, 725, 846
HpaII CCGG 1 cut(s) 224
HphI GGTGA 5 cut(s) 272, 353, 367, 380, 751
Hpy166II GTNNAC 1 cut(s) 375
Hpy188I TCNGA 4 cut(s) 82, 166, 284, 653
Hpy188III TCNNGA 1 cut(s) 875
Hpy8I GTNNAC 1 cut(s) 375
HpyAV CCTTC 7 cut(s) 28, 95, 223, 232, 250, 650, 872
HpyCH4III ACNGT 1 cut(s) 814
HpyCH4V TGCA 3 cut(s) 17, 731, 736
HpyF10VI GCNNNNNNNGC 5 cut(s) 91, 125, 179, 489, 498
HpyF3I CTNAG 5 cut(s) 318, 426, 531, 586, 633
Hsp92II CATG 3 cut(s) 94, 447, 703
HspAI GCGC 1 cut(s) 114
Kzo9I GATC 3 cut(s) 10, 673, 871
LmnI GCTCC 4 cut(s) 82, 99, 586, 710
Lsp1109I GCAGC 1 cut(s) 103
LweI GCATC 2 cut(s) 253, 527
MaeI CTAG 1 cut(s) 843
MaeIII GTNAC 4 cut(s) 104, 722, 739, 824
MalI GATC 3 cut(s) 12, 675, 873
MboI GATC 3 cut(s) 10, 673, 871
MboII GAAGA 8 cut(s) 148, 236, 245, 265, 481, 587, 624, 805
MhlI GDGCHC 3 cut(s) 77, 707, 772
MlsI TGGCCA 1 cut(s) 29
MluCI AATT 5 cut(s) 157, 213, 523, 568, 864
MluNI TGGCCA 1 cut(s) 29
MlyI GAGTC 2 cut(s) 466, 719
MmeI TCCRAC 3 cut(s) 190, 631, 666
Mox20I TGGCCA 1 cut(s) 29
MscI TGGCCA 1 cut(s) 29
MseI TTAA 1 cut(s) 507
MslI CAYNNNNRTG 1 cut(s) 552
Msp20I TGGCCA 1 cut(s) 29
MspI CCGG 1 cut(s) 224
MspR9I CCNGG 2 cut(s) 224, 711
MvaI CCWGG 1 cut(s) 711
MwoI GCNNNNNNNGC 5 cut(s) 91, 125, 179, 489, 498
NciI CCSGG 1 cut(s) 224
NdeII GATC 3 cut(s) 10, 673, 871
NlaIII CATG 3 cut(s) 94, 447, 703
NlaIV GGNNCC 1 cut(s) 74
NmeAIII GCCGAG 1 cut(s) 464
NmuCI GTSAC 2 cut(s) 722, 739
PaqCI CACCTGC 1 cut(s) 727
PasI CCCWGGG 1 cut(s) 710
PfeI GAWTC 2 cut(s) 167, 846
PfoI TCCNGGA 1 cut(s) 222
PkrI GCNGC 1 cut(s) 118
PleI GAGTC 2 cut(s) 466, 719
PpsI GAGTC 2 cut(s) 466, 719
Psp6I CCWGG 1 cut(s) 709
PspGI CCWGG 1 cut(s) 709
PspN4I GGNNCC 1 cut(s) 74
RsaI GTAC 1 cut(s) 397
RsaNI GTAC 1 cut(s) 396
RseI CAYNNNNRTG 1 cut(s) 552
SaqAI TTAA 1 cut(s) 507
SatI GCNGC 1 cut(s) 117
Sau3AI GATC 3 cut(s) 10, 673, 871
SchI GAGTC 2 cut(s) 466, 719
ScrFI CCNGG 2 cut(s) 224, 711
SduI GDGCHC 3 cut(s) 77, 707, 772
SfaNI GCATC 2 cut(s) 253, 527
SmiMI CAYNNNNRTG 1 cut(s) 552
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
Sse9I AATT 5 cut(s) 157, 213, 523, 568, 864
SsiI CCGC 1 cut(s) 492
SspMI CTAG 1 cut(s) 843
StyD4I CCNGG 2 cut(s) 222, 709
StyI CCWWGG 1 cut(s) 193
TaaI ACNGT 1 cut(s) 814
TasI AATT 5 cut(s) 157, 213, 523, 568, 864
TfiI GAWTC 2 cut(s) 167, 846
Tru1I TTAA 1 cut(s) 507
Tru9I TTAA 1 cut(s) 507
TscAI CASTG 5 cut(s) 255, 322, 397, 418, 766
TseFI GTSAC 2 cut(s) 722, 739
TseI GCWGC 1 cut(s) 116
Tsp45I GTSAC 2 cut(s) 722, 739
TspDTI ATGAA 1 cut(s) 823
TspGWI ACGGA 2 cut(s) 86, 705
TspRI CASTG 5 cut(s) 255, 322, 397, 418, 766
XspI CTAG 1 cut(s) 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.