MD03G1100900.v1.1

Glutelin type-A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001439
Physical Location & Seq
Forward (+)
8603175 .. 8605331
2157 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1100900.v1.1.491

Sequence Viewer

Length: 1068 bp
ATGGAGCTTGATCTTTCACCCAAGTTGGCCAAGAAGGTGTACGGCGGCGATGGCGGGTCCTACTTTGCCTGGTCCCCGTCGGAGCTTCCCATGCTCCGTGAAGGTAACATCGGAGCCGCCAAGCTCGCTCTGGACAAGGACGGGTTCGCTCTCCCCAAGTACTCTGACTCCAACCAGGTCGCCTATGTCCTCCAAGGTTCTGGTGTAGTCGGAATTGTCCTCCCGGAGAAGGAAGAGAAGGTTCTTCCAGTTAAGAAGGGTGATGCGATTGCCCTCCCTTTCGGAGTTGTGACTTGGTGGTACAACAAGGAGGACACCGAGTTCGTTGTTCTTTTCTTGGGCGACACTTCAAAAGCTCACAAGAGGGGGGAGTTTACCGACTTCTATCTCAACGGCTCCAACGCCATTTTCACCGGCTTCTCAACCGAGTTTGTTGGTCGGGCATGGGATTTGGAGGAGAGTGTTGTGAAGACTCTCGTTGGCAAGCAGTCCGGCAGGGGCATTGTCAAGCTTACCGGAGCTAACTTGCCCGAGCCAAAGAAGGAACACCGGGATGGCATGACGTTGAACTGCGAAGAGGCTCCATTGGATGTTGACATTAAGGATGGTGGAAGAGTTGTGGTCTTGAATACCAAAAACCTTCCTTTGGTCGGTGAGGTTGGGCTCGGTGCTGACCTTGTGAGATTGGACGGGAGTGCCATGTGCTCCCCTGGATTTTCTTGTGACTCGGCTCTGCAGGTGACTTATATTATTAGGGGCAGCGGCCGTGTCCAGGTTGTTGGTGTCGACGGTAAGAGGGTGTTGGAAACTACCATCAAAGCCGGTAATCTCTTCATTGTTCCTCGGTTCTTTGTTGTGTCAAAGATTGCTGATCCGGAGGGCTTGGAATGGTTTTCTATCATCACTACGCCCAACCCGATATTCACCCACTTGGCTGGAAGCATTGGGTGCTGGAAGGCTCTATCTCCGCAGGTGCTCCAGGCTGCGTTCAATGTGGATTCGGAAACAGAGAAACTCTTCCGTTCGAAGAGGACTTGTGATGCAATCTTCTTCCCCCCACCCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

356

Amino Acids

38.51

Weight (kDa)

5.81

Isoelectric Point (pI)

33.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 11 - 156 8e-24 Cupin
Cupin_1 PF00190 197 - 338 1.9e-24 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 727, 961
Acc36I ACCTGC 2 cut(s) 727, 961
AccI GTMKAC 1 cut(s) 786
AccIII TCCGGA 1 cut(s) 874
AciI CCGC 5 cut(s) 45, 54, 117, 762, 968
AclWI GGATC 1 cut(s) 866
AcoI YGGCCR 2 cut(s) 27, 763
AfaI GTAC 3 cut(s) 41, 161, 302
AfiI CCNNNNNNNGG 4 cut(s) 229, 646, 650, 772
AgsI TTSAA 4 cut(s) 351, 568, 628, 991
AjnI CCWGG 5 cut(s) 68, 174, 709, 771, 978
AjuI GAANNNNNNNTTGG 4 cut(s) 392, 424, 905, 937
AleI CACNNNNGTG 1 cut(s) 1063
AloI GAACNNNNNNTCC 2 cut(s) 305, 337
AluBI AGCT 6 cut(s) 7, 85, 124, 356, 511, 521
AluI AGCT 6 cut(s) 7, 85, 124, 356, 511, 521
Alw21I GWGCWC 2 cut(s) 707, 978
AlwI GGATC 1 cut(s) 866
Ama87I CYCGRG 1 cut(s) 530
Aor13HI TCCGGA 1 cut(s) 874
AoxI GGCC 2 cut(s) 27, 763
ApeKI GCWGC 2 cut(s) 759, 983
ArsI GACNNNNNNTTYG 2 cut(s) 305, 337
Asp700I GAANNNNTTC 1 cut(s) 1016
AspS9I GGNCC 2 cut(s) 57, 72
AsuC2I CCSGG 2 cut(s) 224, 551
AsuHPI GGTGA 6 cut(s) 9, 272, 403, 665, 751, 916
AsuII TTCGAA 1 cut(s) 1025
AvaI CYCGRG 1 cut(s) 530
AvaII GGWCC 2 cut(s) 57, 72
BalI TGGCCA 1 cut(s) 29
BanII GRGCYC 1 cut(s) 666
BbsI GAAGAC 1 cut(s) 476
Bbv12I GWGCWC 2 cut(s) 707, 978
BbvI GCAGC 2 cut(s) 771, 970
BccI CCATC 4 cut(s) 44, 548, 599, 821
BceAI ACGGC 3 cut(s) 58, 409, 750
BciT130I CCWGG 5 cut(s) 70, 176, 711, 773, 980
BcnI CCSGG 2 cut(s) 224, 551
BfmI CTRYAG 1 cut(s) 734
BfuAI ACCTGC 2 cut(s) 727, 961
BisI GCNGC 5 cut(s) 46, 117, 760, 763, 984
BlsI GCNGC 5 cut(s) 47, 118, 761, 764, 985
BmcAI AGTACT 1 cut(s) 161
Bme1390I CCNGG 7 cut(s) 70, 176, 224, 551, 711, 773, 980
Bme18I GGWCC 2 cut(s) 57, 72
BmeT110I CYCGRG 1 cut(s) 530
BmgT120I GGNCC 2 cut(s) 57, 72
BmiI GGNNCC 5 cut(s) 58, 74, 115, 397, 582
BmrFI CCNGG 7 cut(s) 70, 176, 224, 551, 711, 773, 980
BmsI GCATC 2 cut(s) 253, 1030
BpiI GAAGAC 1 cut(s) 476
BpmI CTGGAG 1 cut(s) 962
Bpu14I TTCGAA 1 cut(s) 1025
BpuMI CCSGG 2 cut(s) 224, 551
BsaJI CCNNGG 3 cut(s) 193, 709, 842
BsaWI WCCGGW 2 cut(s) 515, 874
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 4 cut(s) 229, 646, 650, 772
Bse118I RCCGGY 2 cut(s) 413, 821
Bse1I ACTGG 1 cut(s) 248
BseAI TCCGGA 1 cut(s) 874
BseBI CCWGG 5 cut(s) 70, 176, 711, 773, 980
BseDI CCNNGG 3 cut(s) 193, 709, 842
BseGI GGATG 3 cut(s) 559, 595, 610
BseLI CCNNNNNNNGG 4 cut(s) 229, 646, 650, 772
BseNI ACTGG 1 cut(s) 248
BseRI GAGGAG 1 cut(s) 470
BseX3I CGGCCG 1 cut(s) 763
BseXI GCAGC 2 cut(s) 771, 970
Bsh1285I CGRYCG 1 cut(s) 766
BshFI GGCC 2 cut(s) 29, 765
BsiEI CGRYCG 1 cut(s) 766
BsiHKAI GWGCWC 2 cut(s) 707, 978
BsiHKCI CYCGRG 1 cut(s) 530
BsiSI CCGG 7 cut(s) 224, 414, 492, 516, 550, 822, 875
BslFI GGGAC 1 cut(s) 58
BslI CCNNNNNNNGG 4 cut(s) 229, 646, 650, 772
BsmFI GGGAC 1 cut(s) 58
BsnI GGCC 2 cut(s) 29, 765
BsoBI CYCGRG 1 cut(s) 530
Bsp119I TTCGAA 1 cut(s) 1025
Bsp1286I GDGCHC 3 cut(s) 666, 707, 978
Bsp13I TCCGGA 1 cut(s) 874
Bsp143I GATC 2 cut(s) 10, 871
BspACI CCGC 5 cut(s) 45, 54, 117, 762, 968
BspANI GGCC 2 cut(s) 29, 765
BspEI TCCGGA 1 cut(s) 874
BspLI GGNNCC 5 cut(s) 58, 74, 115, 397, 582
BspMAI CTGCAG 1 cut(s) 738
BspMI ACCTGC 2 cut(s) 727, 961
BspPI GGATC 1 cut(s) 866
BspT104I TTCGAA 1 cut(s) 1025
BsrFI RCCGGY 2 cut(s) 413, 821
BsrI ACTGG 1 cut(s) 248
BssAI RCCGGY 2 cut(s) 413, 821
BssECI CCNNGG 3 cut(s) 193, 709, 842
BssMI GATC 2 cut(s) 10, 871
BssT1I CCWWGG 1 cut(s) 193
Bst2UI CCWGG 5 cut(s) 70, 176, 711, 773, 980
Bst4CI ACNGT 1 cut(s) 791
Bst6I CTCTTC 6 cut(s) 228, 570, 607, 836, 1022, 1022
BstAPI GCANNNNNTGC 1 cut(s) 948
BstBI TTCGAA 1 cut(s) 1025
BstC8I GCNNGC 2 cut(s) 126, 485
BstF5I GGATG 3 cut(s) 559, 595, 610
BstKTI GATC 2 cut(s) 13, 874
BstMBI GATC 2 cut(s) 10, 871
BstMCI CGRYCG 1 cut(s) 766
BstMWI GCNNNNNNNGC 4 cut(s) 51, 91, 125, 948
BstNI CCWGG 5 cut(s) 70, 176, 711, 773, 980
BstSCI CCNGG 7 cut(s) 68, 174, 222, 549, 709, 771, 978
BstSFI CTRYAG 1 cut(s) 734
BstV1I GCAGC 2 cut(s) 771, 970
BstV2I GAAGAC 1 cut(s) 476
BstXI CCANNNNNNTGG 3 cut(s) 200, 779, 935
BstZI CGGCCG 1 cut(s) 763
BsuRI GGCC 2 cut(s) 29, 765
BtgZI GCGATG 1 cut(s) 63
BtsCI GGATG 3 cut(s) 559, 595, 610
BveI ACCTGC 2 cut(s) 727, 961
Cac8I GCNNGC 2 cut(s) 126, 485
Cfr10I RCCGGY 2 cut(s) 413, 821
Cfr13I GGNCC 2 cut(s) 57, 72
CsiI ACCWGGT 1 cut(s) 174
Csp6I GTAC 3 cut(s) 40, 160, 301
CviAII CATG 4 cut(s) 91, 444, 559, 700
CviQI GTAC 3 cut(s) 40, 160, 301
DpnI GATC 2 cut(s) 12, 873
DpnII GATC 2 cut(s) 10, 871
EaeI YGGCCR 2 cut(s) 27, 763
EagI CGGCCG 1 cut(s) 763
Eam1104I CTCTTC 6 cut(s) 228, 570, 607, 836, 1022, 1022
EarI CTCTTC 6 cut(s) 228, 570, 607, 836, 1022, 1022
EclXI CGGCCG 1 cut(s) 763
Eco130I CCWWGG 1 cut(s) 193
Eco24I GRGCYC 1 cut(s) 666
Eco47I GGWCC 2 cut(s) 57, 72
Eco52I CGGCCG 1 cut(s) 763
Eco88I CYCGRG 1 cut(s) 530
EcoO109I RGGNCCY 1 cut(s) 57
EcoRII CCWGG 5 cut(s) 68, 174, 709, 771, 978
EcoT14I CCWWGG 1 cut(s) 193
EcoT38I GRGCYC 1 cut(s) 666
ErhI CCWWGG 1 cut(s) 193
FaeI CATG 4 cut(s) 94, 447, 562, 703
FaiI YATR 6 cut(s) 92, 186, 445, 560, 701, 747
FaqI GGGAC 1 cut(s) 58
FatI CATG 4 cut(s) 90, 443, 558, 699
FauI CCCGC 1 cut(s) 47
FblI GTMKAC 1 cut(s) 786
Fnu4HI GCNGC 5 cut(s) 46, 117, 760, 763, 984
FokI GGATG 3 cut(s) 566, 602, 617
FriOI GRGCYC 1 cut(s) 666
Fsp4HI GCNGC 5 cut(s) 46, 117, 760, 763, 984
GluI GCNGC 5 cut(s) 46, 117, 760, 763, 984
GsuI CTGGAG 1 cut(s) 962
HaeIII GGCC 2 cut(s) 29, 765
HapII CCGG 7 cut(s) 224, 414, 492, 516, 550, 822, 875
Hin1II CATG 4 cut(s) 94, 447, 562, 703
HincII GTYRAC 2 cut(s) 595, 787
HindII GTYRAC 2 cut(s) 595, 787
HindIII AAGCTT 1 cut(s) 509
HinfI GANTC 4 cut(s) 167, 472, 725, 998
HpaII CCGG 7 cut(s) 224, 414, 492, 516, 550, 822, 875
HphI GGTGA 6 cut(s) 9, 272, 403, 665, 751, 916
Hpy166II GTNNAC 4 cut(s) 40, 375, 595, 787
Hpy188I TCNGA 6 cut(s) 82, 113, 166, 212, 284, 1003
Hpy188III TCNNGA 3 cut(s) 131, 625, 875
Hpy8I GTNNAC 4 cut(s) 40, 375, 595, 787
Hpy99I CGWCG 2 cut(s) 82, 791
HpyAV CCTTC 8 cut(s) 28, 95, 223, 232, 250, 535, 650, 949
HpyCH4III ACNGT 1 cut(s) 791
HpyCH4IV ACGT 1 cut(s) 563
HpyCH4V TGCA 2 cut(s) 736, 1043
HpyF10VI GCNNNNNNNGC 4 cut(s) 51, 91, 125, 948
HpySE526I ACGT 1 cut(s) 563
Hsp92II CATG 4 cut(s) 94, 447, 562, 703
Kpn2I TCCGGA 1 cut(s) 874
Kzo9I GATC 2 cut(s) 10, 871
LmnI GCTCC 9 cut(s) 4, 82, 99, 113, 401, 518, 586, 710, 981
Lsp1109I GCAGC 2 cut(s) 771, 970
LweI GCATC 2 cut(s) 253, 1030
MabI ACCWGGT 1 cut(s) 174
MaeII ACGT 1 cut(s) 563
MaeIII GTNAC 4 cut(s) 104, 289, 722, 739
MalI GATC 2 cut(s) 12, 873
MboI GATC 2 cut(s) 10, 871
MhlI GDGCHC 3 cut(s) 666, 707, 978
MlsI TGGCCA 1 cut(s) 29
MluCI AATT 1 cut(s) 213
MluNI TGGCCA 1 cut(s) 29
MlyI GAGTC 3 cut(s) 161, 466, 719
MmeI TCCRAC 5 cut(s) 60, 190, 195, 423, 783
Mox20I TGGCCA 1 cut(s) 29
MroI TCCGGA 1 cut(s) 874
MroXI GAANNNNTTC 1 cut(s) 1016
MscI TGGCCA 1 cut(s) 29
MseI TTAA 2 cut(s) 252, 600
MslI CAYNNNNRTG 2 cut(s) 552, 1063
Msp20I TGGCCA 1 cut(s) 29
MspA1I CMGCKG 1 cut(s) 762
MspI CCGG 7 cut(s) 224, 414, 492, 516, 550, 822, 875
MspR9I CCNGG 7 cut(s) 70, 176, 224, 551, 711, 773, 980
MvaI CCWGG 5 cut(s) 70, 176, 711, 773, 980
MwoI GCNNNNNNNGC 4 cut(s) 51, 91, 125, 948
NciI CCSGG 2 cut(s) 224, 551
NdeII GATC 2 cut(s) 10, 871
NlaIII CATG 4 cut(s) 94, 447, 562, 703
NlaIV GGNNCC 5 cut(s) 58, 74, 115, 397, 582
NmeAIII GCCGAG 1 cut(s) 707
NmuCI GTSAC 3 cut(s) 289, 722, 739
NspV TTCGAA 1 cut(s) 1025
OliI CACNNNNGTG 1 cut(s) 1063
PaqCI CACCTGC 2 cut(s) 727, 961
PcsI WCGNNNNNNNCGW 1 cut(s) 914
PdmI GAANNNNTTC 1 cut(s) 1016
PfeI GAWTC 1 cut(s) 998
PfoI TCCNGGA 1 cut(s) 222
PkrI GCNGC 5 cut(s) 47, 118, 761, 764, 985
PleI GAGTC 3 cut(s) 161, 466, 719
PpsI GAGTC 3 cut(s) 161, 466, 719
PpuMI RGGWCCY 1 cut(s) 57
Psp5II RGGWCCY 1 cut(s) 57
Psp6I CCWGG 5 cut(s) 68, 174, 709, 771, 978
PspGI CCWGG 5 cut(s) 68, 174, 709, 771, 978
PspN4I GGNNCC 5 cut(s) 58, 74, 115, 397, 582
PspPI GGNCC 2 cut(s) 57, 72
PspPPI RGGWCCY 1 cut(s) 57
PstI CTGCAG 1 cut(s) 738
RsaI GTAC 3 cut(s) 41, 161, 302
RsaNI GTAC 3 cut(s) 40, 160, 301
RseI CAYNNNNRTG 2 cut(s) 552, 1063
SalI GTCGAC 1 cut(s) 785
SaqAI TTAA 2 cut(s) 252, 600
SatI GCNGC 5 cut(s) 46, 117, 760, 763, 984
Sau3AI GATC 2 cut(s) 10, 871
Sau96I GGNCC 2 cut(s) 57, 72
ScaI AGTACT 1 cut(s) 161
SchI GAGTC 3 cut(s) 161, 466, 719
ScrFI CCNGG 7 cut(s) 70, 176, 224, 551, 711, 773, 980
SduI GDGCHC 3 cut(s) 666, 707, 978
SexAI ACCWGGT 1 cut(s) 174
SfaNI GCATC 2 cut(s) 253, 1030
SfcI CTRYAG 1 cut(s) 734
SfuI TTCGAA 1 cut(s) 1025
SinI GGWCC 2 cut(s) 57, 72
SmiMI CAYNNNNRTG 2 cut(s) 552, 1063
Sse9I AATT 1 cut(s) 213
SsiI CCGC 5 cut(s) 45, 54, 117, 762, 968
StyD4I CCNGG 7 cut(s) 68, 174, 222, 549, 709, 771, 978
StyI CCWWGG 1 cut(s) 193
TaaI ACNGT 1 cut(s) 791
TaiI ACGT 1 cut(s) 566
TaqI TCGA 2 cut(s) 786, 1025
TasI AATT 1 cut(s) 213
TatI WGTACW 1 cut(s) 159
TauI GCSGC 3 cut(s) 48, 119, 765
TfiI GAWTC 1 cut(s) 998
Tru1I TTAA 2 cut(s) 252, 600
Tru9I TTAA 2 cut(s) 252, 600
TseFI GTSAC 3 cut(s) 289, 722, 739
TseI GCWGC 2 cut(s) 759, 983
Tsp45I GTSAC 3 cut(s) 289, 722, 739
TspDTI ATGAA 1 cut(s) 823
TspGWI ACGGA 2 cut(s) 86, 1010
VpaK11BI GGWCC 2 cut(s) 57, 72
XcmI CCANNNNNNNNNTGG 1 cut(s) 127
XmiI GTMKAC 1 cut(s) 786
XmnI GAANNNNTTC 1 cut(s) 1016
ZrmI AGTACT 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.