AT3G06620

kinase) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000660
Physical Location & Seq
Reverse (-)
2062131 .. 2067417
5287 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G06620.1

Sequence Viewer

Length: 2322 bp
ATGGAGAATCCACCTGCGGAGGAGCTGCTGAAGAAGATACTTGAGCTAGAGGAGAGTCAGGAGCATCTCAAGCAGGAGATGTCTAGGCTTAAGGTATCTACGGAGCTTAGACAGCGATCGCATTCGGTGTCTCCTCATCGTCCGGCGAGGAGAAACATCGGAGAGGGAGCTCCGTCTTGGAGGAAAAGCGGTGCCGCCTCGTTTCGTAACGCGTCGCCGTTGCGGAAGGAGAGCCGTATCCAGAATTCGATGAGATTGAGGTCTGAAGTTGGTGGCGGGGGACCATCTGCTGGGAAATTCACTGATAAACAGTATTTGAATATTTTGCAGTCGATGGCACAAGCTGTTCATGCCTTCGATCTTAATATGCGGATTATCTTTTGGAATGCTATGGCGGAAAAGGTTTATGGGTACTCTGCAGCAGAAGCACTTGGGGAGAACCCTATTAATGTTATTGCAGATGATCGGGACGCTGCCTTTGCAATGAATATTGCTCGACGTTGTGTCCGTGGAGAGAGCTGGACTGGTGAGTTTCCTGTTAAGAGCAAATCAGGGGATAGATTTTCAGCTGTCACTACCTGTTCCCCTTTCTATGATGATGATGGTGCTCTTATGGGGATCATTTGTATCACCAGTAACACGGCACCGTATCTGAACCCAAGAATCTCTTTGGCTAAATTGAAGGCGCAAGAAGAAGGTGAAACGAGCTCTATCCCTGCAAGGAATAGTTTTGCCTCTAAACTTGGCTTGGATTCCAGAGGAGCTGTTATATCGAAACTTGGCCTTGACTCTGATCAGCCTATACAAGTTGCTATAGCATCAAAGATCTCAGATTTGGCATCCAAGGTCAGCAACAAGGTCAGGTCGAAAATGCGAGCAGGTGATAATAGTGCCACACTCTCGGAGGGTGGCAGTGGGGATAGTCATCAAAAAGATCATAATGTCTTCGGTGCTACTCTTGTTGACCACAGGGACGATGCAGCATCAAGTGGTGCCAGCACACCAAGAGGGGATTTTATCCAGTCTCCTTTTGGTGTATTCACATGTAATGATGAGAAGTTTGTTTCGAAACCCTTCAAAGATTCCAGTGATGAGAGTGATGGAAAGCCTGCAATCCATAAGGTTCTCACCTCAAAAGCTGAAGAGTGGATGGTAAAGAAAGGTTTGTCATGGCCATGGAAAGGGAATGAGCAGGAAGGTTCAAAAGGAAGACCTACAAATTCTGTTTGGCCTTGGGTACAGAATGAACAAAAGAAAGAGAGGTGTCACCAGATTAATCCCTCTGCTGGTGTTCAGTATGAAAGCCATGCCTTTGAAAGTAATAAGCCTATCAATAATGAGGCTTCTAGTTTGTGGTCTTCCCCTATAAATGCAAACAGTACAAGCAGCGCTAGTAGCTGCGGAAGTACCAGCAGCAGTGTTATGAACAAGGTTGATACTGATAGCGAAGGCTTGGAATATGAAATTTTATGGGATGATTTGACAATTGGAGAACAAGTCGGACAAGGTTCATGTGGAACTGTTTATCACGGTCTCTGGTTTGGATCTGATGTAGCTGTAAAAGTGTTCTCCAAACAAGAATATTCAGCAGAGGTCATAGAATCTTTTAAACAAGAGGTATTGTTGATGAAAAGACTTAGACACCCTAACGTCCTGCTGTTTATGGGAGCTGTGACTTCACCCCAGCGTCTCTGTATAGTGTCAGAGTTCCTTCCACGTGGAAGTCTCTTCCGTCTACTACAGAAGAGCACGTCAAAACTGGATTGGAGGCGGCGTATCCATATGGCCTTGGACATTGCTCGCGGTATGAATTATCTTCACCATTGTAGTCCACCCATTATCCATCGTGATCTGAAGTCATCAAATCTGCTGGTAGACAAGAACTGGACCGTTAAGGTAGCTGACTTTGGTCTCTCGCGTATCAAGCATGAAACATACCTAACCAGCAAGTCTGGGAAGGGAACGCCTCAATGGATGGCACCAGAAGTTCTTCGAAATGAGTCTGCTGATGAGAAATCTGACATTTACAGCTTTGGAGTAGTATTATGGGAGCTTGCCACCGAGAAGATCCCATGGGAAACTCTCAACTCTATGCAGGTGATTGGAGCTGTGGGGTTCATGGACCAGAGGCTGGAAATCCCAAAAGACATTGATCCTCGTTGGATCTCATTAATGGAGAGCTGTTGGCACAGTGATACAAAGCTGAGACCCACATTCCAAGAACTGATGGATAAATTAAGAGACCTGCAAAGAAAGTATATGATACAGTTCCAAGCGACTCGTGCTGCGTTATCTGACAATTCTCTTCTCAAGGACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS PAS_9 Pkinase_Tyr

Protein Analysis

773

Amino Acids

86.05

Weight (kDa)

8.16

Isoelectric Point (pI)

45.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS PF00989 104 - 210 2.2e-12 PAS fold
PAS_4 PF08448 110 - 211 2e-07 PAS fold
PAS_9 PF13426 121 - 210 7.7e-09 PAS domain
PAS_3 PF08447 125 - 210 2.2e-06 PAS fold
PK_Tyr_Ser-Thr PF07714 494 - 746 1.3e-68 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 496 - 744 9.9e-56 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 22, 869, 2086
Acc36I ACCTGC 4 cut(s) 22, 869, 2086, 2253
AccB1I GGYRCC 4 cut(s) 191, 643, 992, 1978
AccB7I CCANNNNNTGG 2 cut(s) 290, 2131
AccI GTMKAC 2 cut(s) 1735, 1875
AccII CGCG 3 cut(s) 212, 1803, 1918
AclWI GGATC 5 cut(s) 626, 1552, 2062, 2147, 2171
AcoI YGGCCR 1 cut(s) 1172
AcsI RAATTY 4 cut(s) 244, 296, 1219, 1464
AcuI CTGAAG 4 cut(s) 50, 285, 1161, 1874
AcvI CACGTG 1 cut(s) 1718
AfaI GTAC 4 cut(s) 413, 1239, 1381, 1408
AfeI AGCGCT 1 cut(s) 1390
AfiI CCNNNNNNNGG 4 cut(s) 290, 1181, 1286, 2131
AflII CTTAAG 1 cut(s) 89
AflIII ACRYGT 2 cut(s) 210, 1043
AgsI TTSAA 5 cut(s) 319, 682, 1078, 1203, 1316
AhdI GACNNNNNGTC 1 cut(s) 503
AjiI CACGTC 1 cut(s) 1752
Alw21I GWGCWC 4 cut(s) 172, 610, 710, 1751
Alw26I GTCTC 8 cut(s) 135, 1029, 1538, 1694, 1730, 1916, 2200, 2235
AlwI GGATC 5 cut(s) 626, 1552, 2062, 2147, 2171
AlwNI CAGNNNCTG 1 cut(s) 2131
Aor51HI AGCGCT 1 cut(s) 1390
AoxI GGCC 4 cut(s) 781, 1172, 1229, 1785
ApeKI GCWGC 8 cut(s) 25, 419, 473, 980, 1386, 1398, 1413, 2285
ApoI RAATTY 4 cut(s) 244, 296, 1219, 1464
ArsI GACNNNNNNTTYG 2 cut(s) 461, 493
AseI ATTAAT 3 cut(s) 447, 1275, 2171
AsiSI GCGATCGC 1 cut(s) 119
Asp700I GAANNNNTTC 2 cut(s) 1073, 1989
AspLEI GCGC 2 cut(s) 688, 1391
AspS9I GGNCC 3 cut(s) 281, 1887, 2122
AsuHPI GGTGA 9 cut(s) 539, 622, 710, 893, 1120, 1259, 1671, 1811, 2110
AsuII TTCGAA 2 cut(s) 1067, 1993
AvaII GGWCC 3 cut(s) 281, 1887, 2122
BalI TGGCCA 1 cut(s) 1174
BanI GGYRCC 4 cut(s) 191, 643, 992, 1978
BanII GRGCYC 2 cut(s) 172, 710
BauI CACGAG 1 cut(s) 2280
BbrPI CACGTG 1 cut(s) 1718
BbsI GAAGAC 3 cut(s) 937, 1216, 1350
Bbv12I GWGCWC 4 cut(s) 172, 610, 710, 1751
BbvI GCAGC 8 cut(s) 12, 431, 460, 992, 1385, 1398, 1425, 2272
BccI CCATC 8 cut(s) 292, 328, 596, 1094, 1144, 1851, 1969, 2221
BceAI ACGGC 3 cut(s) 202, 219, 657
BciVI GTATCC 2 cut(s) 248, 1787
BclI TGATCA 1 cut(s) 793
BcoDI GTCTC 8 cut(s) 135, 1029, 1538, 1694, 1730, 1916, 2200, 2235
BfaI CTAG 4 cut(s) 47, 84, 1347, 1392
BfmI CTRYAG 3 cut(s) 417, 813, 1739
BfoI RGCGCY 1 cut(s) 1392
BfrI CTTAAG 1 cut(s) 89
BfuAI ACCTGC 4 cut(s) 22, 869, 2086, 2253
BfuI GTATCC 2 cut(s) 248, 1787
BglII AGATCT 1 cut(s) 825
Bme18I GGWCC 3 cut(s) 281, 1887, 2122
BmeRI GACNNNNNGTC 1 cut(s) 503
BmgBI CACGTC 1 cut(s) 1752
BmgT120I GGNCC 3 cut(s) 281, 1887, 2122
BmiI GGNNCC 5 cut(s) 193, 282, 645, 994, 1980
BmsI GCATC 5 cut(s) 73, 827, 848, 967, 992
BoxI GACNNNNGTC 1 cut(s) 1908
BpiI GAAGAC 3 cut(s) 937, 1216, 1350
Bpu14I TTCGAA 2 cut(s) 1067, 1993
BpuEI CTTGAG 3 cut(s) 53, 62, 2294
BsaAI YACGTR 1 cut(s) 1718
BsaBI GATNNNNATC 1 cut(s) 924
BsaI GGTCTC 4 cut(s) 1538, 1916, 2200, 2235
BsaJI CCNNGG 6 cut(s) 508, 843, 1175, 1232, 1788, 2072
BsaXI ACNNNNNCTCC 4 cut(s) 1482, 1512, 1759, 1789
Bsc4I CCNNNNNNNGG 4 cut(s) 290, 1181, 1286, 2131
Bse1I ACTGG 6 cut(s) 529, 633, 1021, 1086, 1764, 1889
Bse3DI GCAATG 2 cut(s) 489, 1794
Bse8I GATNNNNATC 1 cut(s) 924
BseDI CCNNGG 6 cut(s) 508, 843, 1175, 1232, 1788, 2072
BseGI GGATG 4 cut(s) 839, 1155, 1480, 1980
BseJI GATNNNNATC 1 cut(s) 924
BseLI CCNNNNNNNGG 4 cut(s) 290, 1181, 1286, 2131
BseMI GCAATG 2 cut(s) 489, 1794
BseMII CTCAG 2 cut(s) 843, 2195
BseNI ACTGG 6 cut(s) 529, 633, 1021, 1086, 1764, 1889
BseRI GAGGAG 5 cut(s) 35, 65, 123, 163, 774
BseXI GCAGC 8 cut(s) 12, 431, 460, 992, 1385, 1398, 1425, 2272
BseYI CCCAGC 2 cut(s) 290, 1683
Bsh1236I CGCG 3 cut(s) 212, 1803, 1918
Bsh1285I CGRYCG 1 cut(s) 119
BshFI GGCC 4 cut(s) 783, 1174, 1231, 1787
BshNI GGYRCC 4 cut(s) 191, 643, 992, 1978
BsiEI CGRYCG 1 cut(s) 119
BsiHKAI GWGCWC 4 cut(s) 172, 610, 710, 1751
BsiSI CCGG 1 cut(s) 143
BslFI GGGAC 3 cut(s) 294, 482, 986
BslI CCNNNNNNNGG 4 cut(s) 290, 1181, 1286, 2131
BsmAI GTCTC 8 cut(s) 135, 1029, 1538, 1694, 1730, 1916, 2200, 2235
BsmBI CGTCTC 1 cut(s) 1694
BsmFI GGGAC 3 cut(s) 294, 482, 986
BsmI GAATGC 2 cut(s) 121, 391
BsnI GGCC 4 cut(s) 783, 1174, 1231, 1787
Bso31I GGTCTC 4 cut(s) 1538, 1916, 2200, 2235
Bsp119I TTCGAA 2 cut(s) 1067, 1993
Bsp1286I GDGCHC 4 cut(s) 172, 610, 710, 1751
Bsp19I CCATGG 2 cut(s) 1175, 2072
BspANI GGCC 4 cut(s) 783, 1174, 1231, 1787
BspCNI CTCAG 2 cut(s) 842, 2196
BspFNI CGCG 3 cut(s) 212, 1803, 1918
BspLI GGNNCC 5 cut(s) 193, 282, 645, 994, 1980
BspMAI CTGCAG 1 cut(s) 421
BspMI ACCTGC 4 cut(s) 22, 869, 2086, 2253
BspPI GGATC 5 cut(s) 626, 1552, 2062, 2147, 2171
BspQI GCTCTTC 1 cut(s) 1739
BspT104I TTCGAA 2 cut(s) 1067, 1993
BspT107I GGYRCC 4 cut(s) 191, 643, 992, 1978
BspTI CTTAAG 1 cut(s) 89
BspTNI GGTCTC 4 cut(s) 1538, 1916, 2200, 2235
BsrDI GCAATG 2 cut(s) 489, 1794
BsrI ACTGG 6 cut(s) 529, 633, 1021, 1086, 1764, 1889
BssECI CCNNGG 6 cut(s) 508, 843, 1175, 1232, 1788, 2072
BssSI CACGAG 1 cut(s) 2280
BssT1I CCWWGG 5 cut(s) 843, 1175, 1232, 1788, 2072
Bst2BI CACGAG 1 cut(s) 2280
Bst4CI ACNGT 8 cut(s) 312, 648, 1379, 1522, 1532, 1891, 2192, 2268
Bst6I CTCTTC 4 cut(s) 1137, 1733, 1739, 2310
BstAFI CTTAAG 1 cut(s) 89
BstBAI YACGTR 1 cut(s) 1718
BstBI TTCGAA 2 cut(s) 1067, 1993
BstC8I GCNNGC 5 cut(s) 876, 997, 1110, 1801, 2055
BstDEI CTNAG 4 cut(s) 107, 829, 1637, 2204
BstDSI CCRYGG 3 cut(s) 508, 1175, 2072
BstF5I GGATG 4 cut(s) 839, 1155, 1480, 1980
BstFNI CGCG 3 cut(s) 212, 1803, 1918
BstH2I RGCGCY 1 cut(s) 1392
BstHHI GCGC 2 cut(s) 688, 1391
BstMAI GTCTC 8 cut(s) 135, 1029, 1538, 1694, 1730, 1916, 2200, 2235
BstMCI CGRYCG 1 cut(s) 119
BstMWI GCNNNNNNNGC 8 cut(s) 70, 112, 350, 425, 479, 1395, 1924, 2282
BstNSI RCATGY 1 cut(s) 1047
BstPAI GACNNNNGTC 1 cut(s) 1908
BstSFI CTRYAG 3 cut(s) 417, 813, 1739
BstUI CGCG 3 cut(s) 212, 1803, 1918
BstV1I GCAGC 8 cut(s) 12, 431, 460, 992, 1385, 1398, 1425, 2272
BstV2I GAAGAC 3 cut(s) 937, 1216, 1350
BstX2I RGATCY 4 cut(s) 825, 1544, 2067, 2163
BstYI RGATCY 4 cut(s) 825, 1544, 2067, 2163
BsuI GTATCC 2 cut(s) 248, 1787
BsuRI GGCC 4 cut(s) 783, 1174, 1231, 1787
BtgI CCRYGG 3 cut(s) 508, 1175, 2072
BtrI CACGTC 1 cut(s) 1752
BtsCI GGATG 4 cut(s) 839, 1155, 1480, 1980
BtsI GCAGTG 2 cut(s) 919, 1423
BtsIMutI CAGTG 5 cut(s) 300, 919, 1093, 1423, 2197
BveI ACCTGC 4 cut(s) 22, 869, 2086, 2253
Cac8I GCNNGC 5 cut(s) 876, 997, 1110, 1801, 2055
CaiI CAGNNNCTG 1 cut(s) 2131
CfoI GCGC 2 cut(s) 688, 1391
Cfr13I GGNCC 3 cut(s) 281, 1887, 2122
CseI GACGC 3 cut(s) 201, 479, 1676
Csp6I GTAC 4 cut(s) 412, 1238, 1380, 1407
CviAII CATG 9 cut(s) 350, 1044, 1170, 1176, 1307, 1512, 1928, 2073, 2119
CviQI GTAC 4 cut(s) 412, 1238, 1380, 1407
DdeI CTNAG 4 cut(s) 107, 829, 1637, 2204
DraI TTTAAA 1 cut(s) 1609
DriI GACNNNNNGTC 1 cut(s) 503
EaeI YGGCCR 1 cut(s) 1172
Eam1104I CTCTTC 4 cut(s) 1137, 1733, 1739, 2310
Eam1105I GACNNNNNGTC 1 cut(s) 503
EarI CTCTTC 4 cut(s) 1137, 1733, 1739, 2310
EciI GGCGGA 1 cut(s) 410
Ecl136II GAGCTC 2 cut(s) 170, 708
Eco130I CCWWGG 5 cut(s) 843, 1175, 1232, 1788, 2072
Eco24I GRGCYC 2 cut(s) 172, 710
Eco31I GGTCTC 4 cut(s) 1538, 1916, 2200, 2235
Eco47I GGWCC 3 cut(s) 281, 1887, 2122
Eco47III AGCGCT 1 cut(s) 1390
Eco53kI GAGCTC 2 cut(s) 170, 708
Eco57I CTGAAG 4 cut(s) 50, 285, 1161, 1874
Eco72I CACGTG 1 cut(s) 1718
EcoICRI GAGCTC 2 cut(s) 170, 708
EcoRI GAATTC 1 cut(s) 244
EcoT14I CCWWGG 5 cut(s) 843, 1175, 1232, 1788, 2072
EcoT38I GRGCYC 2 cut(s) 172, 710
ErhI CCWWGG 5 cut(s) 843, 1175, 1232, 1788, 2072
Esp3I CGTCTC 1 cut(s) 1694
FaeI CATG 9 cut(s) 353, 1047, 1173, 1179, 1310, 1515, 1931, 2076, 2122
FalI AAGNNNNNCTT 2 cut(s) 652, 684
FaqI GGGAC 3 cut(s) 294, 482, 986
FatI CATG 9 cut(s) 349, 1043, 1169, 1175, 1306, 1511, 1927, 2072, 2118
FauI CCCGC 1 cut(s) 269
FauNDI CATATG 1 cut(s) 1782
FbaI TGATCA 1 cut(s) 793
FblI GTMKAC 2 cut(s) 1735, 1875
FokI GGATG 4 cut(s) 826, 1162, 1487, 1987
FriOI GRGCYC 2 cut(s) 172, 710
FspBI CTAG 4 cut(s) 47, 84, 1347, 1392
GlaI GCGC 2 cut(s) 687, 1390
GsaI CCCAGC 2 cut(s) 294, 1687
HaeII RGCGCY 1 cut(s) 1392
HaeIII GGCC 4 cut(s) 783, 1174, 1231, 1787
HapII CCGG 1 cut(s) 143
HgaI GACGC 3 cut(s) 201, 479, 1676
HhaI GCGC 2 cut(s) 688, 1391
Hin1II CATG 9 cut(s) 353, 1047, 1173, 1179, 1310, 1515, 1931, 2076, 2122
Hin6I GCGC 2 cut(s) 686, 1389
HinP1I GCGC 2 cut(s) 686, 1389
HincII GTYRAC 1 cut(s) 964
HindII GTYRAC 1 cut(s) 964
HinfI GANTC 9 cut(s) 7, 55, 663, 752, 788, 1082, 1601, 2000, 2278
HpaII CCGG 1 cut(s) 143
HphI GGTGA 9 cut(s) 539, 622, 710, 893, 1120, 1259, 1671, 1811, 2110
Hpy166II GTNNAC 4 cut(s) 964, 1736, 1832, 1876
Hpy188III TCNNGA 5 cut(s) 59, 241, 467, 756, 1847
Hpy8I GTNNAC 4 cut(s) 964, 1736, 1832, 1876
Hpy99I CGWCG 2 cut(s) 217, 501
HpyAV CCTTC 9 cut(s) 220, 364, 676, 689, 1084, 1190, 1442, 1721, 1951
HpyCH4III ACNGT 8 cut(s) 312, 648, 1379, 1522, 1532, 1891, 2192, 2268
HpyCH4IV ACGT 4 cut(s) 499, 1650, 1717, 1751
HpyF10VI GCNNNNNNNGC 8 cut(s) 70, 112, 350, 425, 479, 1395, 1924, 2282
HpyF3I CTNAG 4 cut(s) 107, 829, 1637, 2204
HpySE526I ACGT 4 cut(s) 499, 1650, 1717, 1751
Hsp92II CATG 9 cut(s) 353, 1047, 1173, 1179, 1310, 1515, 1931, 2076, 2122
HspAI GCGC 2 cut(s) 686, 1389
Ksp22I TGATCA 1 cut(s) 793
LguI GCTCTTC 1 cut(s) 1739
LmnI GCTCC 9 cut(s) 22, 61, 103, 167, 175, 761, 1667, 2050, 2105
Lsp1109I GCAGC 8 cut(s) 12, 431, 460, 992, 1385, 1398, 1425, 2272
LweI GCATC 5 cut(s) 73, 827, 848, 967, 992
MaeI CTAG 4 cut(s) 47, 84, 1347, 1392
MaeII ACGT 4 cut(s) 499, 1650, 1717, 1751
MaeIII GTNAC 5 cut(s) 206, 571, 635, 1265, 1672
MfeI CAATTG 1 cut(s) 1485
MflI RGATCY 4 cut(s) 825, 1544, 2067, 2163
MhlI GDGCHC 4 cut(s) 172, 610, 710, 1751
MlsI TGGCCA 1 cut(s) 1174
MluCI AATT 9 cut(s) 244, 296, 677, 1219, 1464, 1485, 1810, 2234, 2299
MluI ACGCGT 1 cut(s) 210
MluNI TGGCCA 1 cut(s) 1174
MlyI GAGTC 4 cut(s) 64, 782, 2009, 2272
MmeI TCCRAC 2 cut(s) 1480, 2141
Mox20I TGGCCA 1 cut(s) 1174
MroXI GAANNNNTTC 2 cut(s) 1073, 1989
MscI TGGCCA 1 cut(s) 1174
MseI TTAA 9 cut(s) 90, 363, 447, 540, 1275, 1608, 1893, 2171, 2237
MslI CAYNNNNRTG 1 cut(s) 1174
Msp20I TGGCCA 1 cut(s) 1174
MspA1I CMGCKG 1 cut(s) 569
MspCI CTTAAG 1 cut(s) 89
MspI CCGG 1 cut(s) 143
MunI CAATTG 1 cut(s) 1485
Mva1269I GAATGC 2 cut(s) 121, 391
MvnI CGCG 3 cut(s) 212, 1803, 1918
MwoI GCNNNNNNNGC 8 cut(s) 70, 112, 350, 425, 479, 1395, 1924, 2282
NcoI CCATGG 2 cut(s) 1175, 2072
NdeI CATATG 1 cut(s) 1782
NlaIII CATG 9 cut(s) 353, 1047, 1173, 1179, 1310, 1515, 1931, 2076, 2122
NlaIV GGNNCC 5 cut(s) 193, 282, 645, 994, 1980
NmuCI GTSAC 3 cut(s) 571, 1265, 1672
NspI RCATGY 1 cut(s) 1047
NspV TTCGAA 2 cut(s) 1067, 1993
PaqCI CACCTGC 3 cut(s) 22, 869, 2086
PciI ACATGT 1 cut(s) 1043
PciSI GCTCTTC 1 cut(s) 1739
PcsI WCGNNNNNNNCGW 1 cut(s) 505
PctI GAATGC 2 cut(s) 121, 391
PdmI GAANNNNTTC 2 cut(s) 1073, 1989
PfeI GAWTC 5 cut(s) 7, 663, 752, 1082, 1601
PflMI CCANNNNNTGG 2 cut(s) 290, 2131
Ple19I CGATCG 1 cut(s) 119
PleI GAGTC 4 cut(s) 63, 782, 2008, 2272
PmaCI CACGTG 1 cut(s) 1718
PmlI CACGTG 1 cut(s) 1718
PpsI GAGTC 4 cut(s) 63, 782, 2008, 2272
Ppu21I YACGTR 1 cut(s) 1718
PscI ACATGT 1 cut(s) 1043
PshAI GACNNNNGTC 1 cut(s) 1908
PshBI ATTAAT 3 cut(s) 447, 1275, 2171
Psp124BI GAGCTC 2 cut(s) 172, 710
PspCI CACGTG 1 cut(s) 1718
PspFI CCCAGC 2 cut(s) 290, 1683
PspN4I GGNNCC 5 cut(s) 193, 282, 645, 994, 1980
PspPI GGNCC 3 cut(s) 281, 1887, 2122
PstI CTGCAG 1 cut(s) 421
PstNI CAGNNNCTG 1 cut(s) 2131
PsuI RGATCY 4 cut(s) 825, 1544, 2067, 2163
PvuI CGATCG 1 cut(s) 119
PvuII CAGCTG 1 cut(s) 569
RgaI GCGATCGC 1 cut(s) 119
RsaI GTAC 4 cut(s) 413, 1239, 1381, 1408
RsaNI GTAC 4 cut(s) 412, 1238, 1380, 1407
RseI CAYNNNNRTG 1 cut(s) 1174
SacI GAGCTC 2 cut(s) 172, 710
SapI GCTCTTC 1 cut(s) 1739
SaqAI TTAA 9 cut(s) 90, 363, 447, 540, 1275, 1608, 1893, 2171, 2237
Sau96I GGNCC 3 cut(s) 281, 1887, 2122
SchI GAGTC 4 cut(s) 64, 782, 2009, 2272
SduI GDGCHC 4 cut(s) 172, 610, 710, 1751
SfaAI GCGATCGC 1 cut(s) 119
SfaNI GCATC 5 cut(s) 73, 827, 848, 967, 992
SfcI CTRYAG 3 cut(s) 417, 813, 1739
SfuI TTCGAA 2 cut(s) 1067, 1993
SgfI GCGATCGC 1 cut(s) 119
SinI GGWCC 3 cut(s) 281, 1887, 2122
SmiMI CAYNNNNRTG 1 cut(s) 1174
SmlI CTYRAG 4 cut(s) 41, 68, 89, 2309
SmoI CTYRAG 4 cut(s) 41, 68, 89, 2309
Sse9I AATT 9 cut(s) 244, 296, 677, 1219, 1464, 1485, 1810, 2234, 2299
SspI AATATT 3 cut(s) 322, 490, 1583
SspMI CTAG 4 cut(s) 47, 84, 1347, 1392
SstI GAGCTC 2 cut(s) 172, 710
StyI CCWWGG 5 cut(s) 843, 1175, 1232, 1788, 2072
TaaI ACNGT 8 cut(s) 312, 648, 1379, 1522, 1532, 1891, 2192, 2268
TaiI ACGT 4 cut(s) 502, 1653, 1720, 1754
TaqI TCGA 8 cut(s) 248, 332, 357, 496, 773, 866, 1067, 1993
TasI AATT 9 cut(s) 244, 296, 677, 1219, 1464, 1485, 1810, 2234, 2299
TatI WGTACW 1 cut(s) 1379
TauI GCSGC 2 cut(s) 197, 1774
TfiI GAWTC 5 cut(s) 7, 663, 752, 1082, 1601
Tru1I TTAA 9 cut(s) 90, 363, 447, 540, 1275, 1608, 1893, 2171, 2237
Tru9I TTAA 9 cut(s) 90, 363, 447, 540, 1275, 1608, 1893, 2171, 2237
TscAI CASTG 5 cut(s) 307, 919, 1093, 1423, 2197
TseFI GTSAC 3 cut(s) 571, 1265, 1672
TseI GCWGC 8 cut(s) 25, 419, 473, 980, 1386, 1398, 1413, 2285
Tsp45I GTSAC 3 cut(s) 571, 1265, 1672
TspGWI ACGGA 4 cut(s) 116, 162, 497, 1721
TspRI CASTG 5 cut(s) 307, 919, 1093, 1423, 2197
Van91I CCANNNNNTGG 2 cut(s) 290, 2131
Vha464I CTTAAG 1 cut(s) 89
VpaK11BI GGWCC 3 cut(s) 281, 1887, 2122
VspI ATTAAT 3 cut(s) 447, 1275, 2171
XapI RAATTY 4 cut(s) 244, 296, 1219, 1464
XceI RCATGY 1 cut(s) 1047
XcmI CCANNNNNNNNNTGG 1 cut(s) 1028
XmiI GTMKAC 2 cut(s) 1735, 1875
XmnI GAANNNNTTC 2 cut(s) 1073, 1989
XspI CTAG 4 cut(s) 47, 84, 1347, 1392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.