FvH4_4g19800

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000660
Physical Location & Seq
Forward (+)
23335261 .. 23343422
8162 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g19800.t2

Sequence Viewer

Length: 2334 bp
ATGGCCACTCCGCCGCCGGCGGAGGAGCTTCTGAAGAAGATCCAGGTACTGGAGGAGGGTCAGGAGAATCTGAAGCAGGAGATGTCCAAGCTCCGGCATACTTCGGATGCTAAGTCGGAGCATCCGAGGTCCCACTCTGTCTCGCCGCAGCGGTCCAGATTCTCGACTTTGCCCAAGAGGAGAGTCGCCGGCGGCGGCGGCGACGCGGCGGCTTGGAAGAAAGGGTCTACCTCTTTTCGGCATTCTTCGCCGCTGCAGAGGGAGAGCCGCAGCCATGATCCTCCCACTGGCGGCAGCACCGGCAGTGGGAAGCGCGGCGGCGGCGGCGATGATTTTAGAGGTGGAGGTGGTAGCGGGCCATCGGCGGTGAACTTTACTGATAGGCAGTATCTGAATATATTGCAGTCCATTGGGCAGTCTGTGCATATTTTTGATCCTCATGGTCGTATAATTTACTGGAACAAAACTGCTGAAGCTCTATATGGTTATTCGGCGGCAGAGGCCCTTGGACATAATCCCATTGAGTTGTTGGCAGATCCCCAGGACTATGTTGTGGGACATAATATAATTGATAGGGTCACCAAGGGGGAGAGCTGGACAGGGCAGTTCCCTGTCAAGAACAAAAAGGGGGAGAGGTTCTTAGCTACTGTGACGAATACTCCATTCTATGATGATGATGGCACTTTTATTGGGGTTATTGCCGTGTCAAGTGATTCAAGGTCATTTCAAGAAATGAGGGTCCCTTTGTCAGGAGTGACGCACCCTGAACCAGATTCAAGCTTCAGTCGTACTAAAGCTACTGTTACAACGAAACTTGGTCTTGATCCTCAGCAGCCTCTGCAAACTGCAATTGCATCAAAAATTACAAACTTGGCGTCCAAGATGAGCATCAAAGTCAAGTCAAAGATTCGGGCAGGAGAAAACTATATGGATCGGGAAGGTGGGAGCGTTGATAGTCATCATTCTGATCATGGTTTGGACTTTGACCATCGGGAGGATGCAAATTCAAGTGGGGCTAGTACACCTAGAGGAGATATAGCCCCATCTCATTTTGGTGTGTTTTCCCAGATGGATGAAAGGTCTCCAGGAAAACCCTCCAGAGATTCTGGCGATGAAAGTGAAGGAAAACCGCCAATCCATAAGGTAATATCGTCAAAGGCAGAAGCGTGGATAGGTAAGAAAGGGATAACTTGGCCATGGAAAGGAAATGAGCGAGAAGGGTCAGAGCCTAAGACCACTCGTTTTGTTTGGCCTTGGTTGCATAATGATCAAGATAATGATACAGTTCCTCAGAAGAGTAGTGGTGGTTTAAAACCTGACAATCATGGAAATGAAAGTAATCGACCTGCAACTAATGAGGCTTCAGGATCCTGGACTTCCTCGTTTAATGTTAACAGTACAAGCAGTGCCAGCAGCTGTGGAAGCACCACCAGTACTGTGAATAAGGTAGATGTGGACACTGATTGCTTAGATTACGAAATCTTGTGGGAAGACTTGATAATTGGAGAACAGATTGGGCAAGGTTCATGTGGAACTGTATACCATGGTCTGTGGTACGGATCAGACGTTGCTGTTAAGGTATTCTCCAGGCAGGAATATTCAGAAGATGTGATACTTTCCTTCAGACAAGAGGTATCTCTAATGAAAAGGCTTCGACATCCAAATGTTCTACTTTTTATGGGAGCTGTGACTTCTCCCGAGCGTCTTTGTATCGTAACAGAGTTCCTCCCACGTGGAAGTTTGTTCCGGTTACTACAAAGAAGCACAGCAAAACTAGACTGGAGACGACGTGTTCATATGGCTCTGGATATTGCACGAGGCATGAATTATCTTCATCATTTCAACCCTCCAATCATCCATCGAGATCTGAAGTCTTCAAATCTCCTAGTTGATAAGAACTGGACTGTCAAGGTTGGTGATTTTGGTCTTTCACGTCTAAAGCACGAAACATTTCTCACAACAAAGACGGGGAGGGGAACGCCTCAGTGGATGGCACCAGAAGTTCTACGCAATGAACCCTCAGATGAGAAGTCGGATGTATACAGCTACGGGGTGATATTGTGGGAGCTTGTTACTGAGAAGATCCCTTGGGATAATCTAAACTCAATGCAGGTGATTGGAGCTGTAGGGTTCATGAACCAAAGACTAGAGATCCCAAAAGATATTGACCCGCAATGGATTTCTTTAATTGAGAGCTGCTGGCACAGTGATCCTGCTTTGCGGCCAACATTTCAAGAGCTGCTGGAAAAGCTTCGAGATCTGCAGAGACAGTATGCCCTTCAATTCCAAGCAGCGCGCTCCGCCGCAGGTGATAGCACCCAAAAAGAACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS PAS_9 Pkinase_Tyr

Protein Analysis

778

Amino Acids

86.22

Weight (kDa)

6.83

Isoelectric Point (pI)

50.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS PF00989 129 - 235 2e-12 PAS fold
PAS_4 PF08448 135 - 238 2.2e-12 PAS fold
PAS_9 PF13426 143 - 238 2.7e-11 PAS domain
PAS_3 PF08447 150 - 235 4.5e-06 PAS fold
PK_Tyr_Ser-Thr PF07714 501 - 701 2.4e-55 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 502 - 704 1e-50 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 2101, 2297
Acc36I ACCTGC 3 cut(s) 1354, 2101, 2297
AccB1I GGYRCC 1 cut(s) 1993
AccB7I CCANNNNNTGG 1 cut(s) 49
AccI GTMKAC 3 cut(s) 227, 1539, 2040
AccII CGCG 3 cut(s) 206, 315, 2296
AcoI YGGCCR 3 cut(s) 3, 1193, 2222
AcsI RAATTY 1 cut(s) 1003
AcuI CTGAAG 7 cut(s) 53, 92, 492, 766, 1347, 1606, 1889
AcvI CACGTG 1 cut(s) 1733
AcyI GRCGYC 1 cut(s) 875
AfaI GTAC 6 cut(s) 48, 790, 1021, 1399, 1435, 1556
AfiI CCNNNNNNNGG 9 cut(s) 49, 93, 237, 287, 290, 306, 749, 994, 1202
AflIII ACRYGT 1 cut(s) 1789
AgsI TTSAA 8 cut(s) 717, 728, 777, 1008, 1843, 1878, 2234, 2282
AjiI CACGTC 2 cut(s) 1790, 1934
AjnI CCWGG 5 cut(s) 42, 540, 1084, 1370, 1586
Alw26I GTCTC 4 cut(s) 145, 1086, 1777, 2260
AlwNI CAGNNNCTG 4 cut(s) 49, 391, 838, 1416
Ama87I CYCGRG 1 cut(s) 1697
AoxI GGCC 6 cut(s) 3, 356, 501, 1193, 1250, 2222
ApeKI GCWGC 9 cut(s) 148, 253, 270, 294, 832, 1413, 2196, 2239, 2291
ApoI RAATTY 1 cut(s) 1003
ArsI GACNNNNNNTTYG 2 cut(s) 860, 892
Asp700I GAANNNNTTC 2 cut(s) 1950, 2250
AspLEI GCGC 3 cut(s) 315, 2296, 2298
AspS9I GGNCC 5 cut(s) 129, 153, 356, 502, 739
AsuHPI GGTGA 6 cut(s) 379, 571, 1928, 2065, 2125, 2321
AvaI CYCGRG 1 cut(s) 1697
AvaII GGWCC 3 cut(s) 129, 153, 739
BalI TGGCCA 2 cut(s) 5, 1195
BamHI GGATCC 1 cut(s) 1367
BanI GGYRCC 1 cut(s) 1993
BarI GAAGNNNNNNTAC 2 cut(s) 1596, 1628
BauI CACGAG 1 cut(s) 1815
BbrPI CACGTG 1 cut(s) 1733
BbsI GAAGAC 2 cut(s) 1497, 1866
BbvCI CCTCAGC 1 cut(s) 828
BbvI GCAGC 9 cut(s) 160, 240, 282, 306, 844, 1425, 2183, 2226, 2303
BccI CCATC 7 cut(s) 367, 671, 996, 1051, 1063, 1866, 1984
BceAI ACGGC 1 cut(s) 686
BciT130I CCWGG 5 cut(s) 44, 542, 1086, 1372, 1588
BclI TGATCA 2 cut(s) 967, 1267
BcoDI GTCTC 4 cut(s) 145, 1086, 1777, 2260
BfaI CTAG 5 cut(s) 1017, 1026, 1775, 1886, 2147
BfmI CTRYAG 3 cut(s) 254, 2124, 2261
BfuAI ACCTGC 3 cut(s) 1354, 2101, 2297
BglII AGATCT 2 cut(s) 1864, 2257
BmcAI AGTACT 1 cut(s) 1435
Bme1390I CCNGG 5 cut(s) 44, 542, 1086, 1372, 1588
Bme18I GGWCC 3 cut(s) 129, 153, 739
BmeT110I CYCGRG 1 cut(s) 1697
BmgBI CACGTC 2 cut(s) 1790, 1934
BmgT120I GGNCC 5 cut(s) 129, 153, 356, 502, 739
BmiI GGNNCC 5 cut(s) 131, 740, 741, 1369, 1995
BmrFI CCNGG 5 cut(s) 44, 542, 1086, 1372, 1588
BmsI GCATC 5 cut(s) 97, 130, 863, 897, 988
BpiI GAAGAC 2 cut(s) 1497, 1866
BpmI CTGGAG 5 cut(s) 71, 1068, 1081, 1570, 1801
Bpu10I CCTNAGC 1 cut(s) 828
BsaAI YACGTR 1 cut(s) 1733
BsaBI GATNNNNATC 2 cut(s) 887, 957
BsaHI GRCGYC 1 cut(s) 875
BsaI GGTCTC 1 cut(s) 1086
BsaJI CCNNGG 8 cut(s) 125, 505, 540, 582, 1196, 1253, 1543, 2087
BsaWI WCCGGW 1 cut(s) 1746
BsaXI ACNNNNNCTCC 4 cut(s) 643, 673, 1774, 1804
Bsc4I CCNNNNNNNGG 9 cut(s) 49, 93, 237, 287, 290, 306, 749, 994, 1202
Bse118I RCCGGY 3 cut(s) 16, 188, 299
Bse1I ACTGG 6 cut(s) 54, 292, 461, 1431, 1784, 1904
Bse3DI GCAATG 2 cut(s) 2017, 2180
Bse8I GATNNNNATC 2 cut(s) 887, 957
BseBI CCWGG 5 cut(s) 44, 542, 1086, 1372, 1588
BseDI CCNNGG 8 cut(s) 125, 505, 540, 582, 1196, 1253, 1543, 2087
BseGI GGATG 8 cut(s) 112, 121, 1003, 1078, 1657, 1854, 1995, 2041
BseJI GATNNNNATC 2 cut(s) 887, 957
BseLI CCNNNNNNNGG 9 cut(s) 49, 93, 237, 287, 290, 306, 749, 994, 1202
BseMI GCAATG 2 cut(s) 2017, 2180
BseMII CTCAG 5 cut(s) 842, 1304, 1997, 2034, 2067
BseNI ACTGG 6 cut(s) 54, 292, 461, 1431, 1784, 1904
BsePI GCGCGC 1 cut(s) 2294
BseRI GAGGAG 4 cut(s) 38, 68, 193, 1044
BseXI GCAGC 9 cut(s) 160, 240, 282, 306, 844, 1425, 2183, 2226, 2303
Bsh1236I CGCG 3 cut(s) 206, 315, 2296
BshFI GGCC 6 cut(s) 5, 358, 503, 1195, 1252, 2224
BshNI GGYRCC 1 cut(s) 1993
BsiHKCI CYCGRG 1 cut(s) 1697
BsiSI CCGG 5 cut(s) 17, 94, 189, 300, 1747
BslFI GGGAC 3 cut(s) 115, 570, 725
BslI CCNNNNNNNGG 9 cut(s) 49, 93, 237, 287, 290, 306, 749, 994, 1202
BsmAI GTCTC 4 cut(s) 145, 1086, 1777, 2260
BsmBI CGTCTC 1 cut(s) 1777
BsmFI GGGAC 3 cut(s) 115, 570, 725
BsmI GAATGC 1 cut(s) 241
BsnI GGCC 6 cut(s) 5, 358, 503, 1195, 1252, 2224
Bso31I GGTCTC 1 cut(s) 1086
BsoBI CYCGRG 1 cut(s) 1697
Bsp19I CCATGG 2 cut(s) 1196, 1543
BspANI GGCC 6 cut(s) 5, 358, 503, 1195, 1252, 2224
BspCNI CTCAG 5 cut(s) 841, 1303, 1996, 2033, 2068
BspFNI CGCG 3 cut(s) 206, 315, 2296
BspHI TCATGA 1 cut(s) 2133
BspLI GGNNCC 5 cut(s) 131, 740, 741, 1369, 1995
BspMAI CTGCAG 2 cut(s) 258, 2265
BspMI ACCTGC 3 cut(s) 1354, 2101, 2297
BspT107I GGYRCC 1 cut(s) 1993
BspTNI GGTCTC 1 cut(s) 1086
BsrDI GCAATG 2 cut(s) 2017, 2180
BsrFI RCCGGY 3 cut(s) 16, 188, 299
BsrI ACTGG 6 cut(s) 54, 292, 461, 1431, 1784, 1904
BssAI RCCGGY 3 cut(s) 16, 188, 299
BssECI CCNNGG 8 cut(s) 125, 505, 540, 582, 1196, 1253, 1543, 2087
BssHII GCGCGC 1 cut(s) 2294
BssNAI GTATAC 2 cut(s) 1540, 2041
BssNI GRCGYC 1 cut(s) 875
BssSI CACGAG 1 cut(s) 1815
BssT1I CCWWGG 6 cut(s) 505, 582, 1196, 1253, 1543, 2087
Bst1107I GTATAC 2 cut(s) 1540, 2041
Bst2BI CACGAG 1 cut(s) 1815
Bst2UI CCWGG 5 cut(s) 44, 542, 1086, 1372, 1588
Bst6I CTCTTC 1 cut(s) 1289
BstACI GRCGYC 1 cut(s) 875
BstAPI GCANNNNNTGC 2 cut(s) 421, 838
BstBAI YACGTR 1 cut(s) 1733
BstC8I GCNNGC 6 cut(s) 18, 190, 356, 1411, 2201, 2296
BstDEI CTNAG 9 cut(s) 111, 640, 828, 1230, 1290, 1468, 1983, 2020, 2076
BstDSI CCRYGG 2 cut(s) 1196, 1543
BstEII GGTNACC 1 cut(s) 577
BstENI CCTNNNNNAGG 1 cut(s) 747
BstF5I GGATG 8 cut(s) 112, 121, 1003, 1078, 1657, 1854, 1995, 2041
BstFNI CGCG 3 cut(s) 206, 315, 2296
BstHHI GCGC 3 cut(s) 315, 2296, 2298
BstMAI GTCTC 4 cut(s) 145, 1086, 1777, 2260
BstNI CCWGG 5 cut(s) 44, 542, 1086, 1372, 1588
BstPI GGTNACC 1 cut(s) 577
BstSCI CCNGG 5 cut(s) 42, 540, 1084, 1370, 1586
BstSFI CTRYAG 3 cut(s) 254, 2124, 2261
BstUI CGCG 3 cut(s) 206, 315, 2296
BstV1I GCAGC 9 cut(s) 160, 240, 282, 306, 844, 1425, 2183, 2226, 2303
BstV2I GAAGAC 2 cut(s) 1497, 1866
BstX2I RGATCY 7 cut(s) 39, 535, 1367, 1864, 2082, 2151, 2257
BstYI RGATCY 7 cut(s) 39, 535, 1367, 1864, 2082, 2151, 2257
BstZ17I GTATAC 2 cut(s) 1540, 2041
BsuRI GGCC 6 cut(s) 5, 358, 503, 1195, 1252, 2224
BtgI CCRYGG 2 cut(s) 1196, 1543
BtgZI GCGATG 2 cut(s) 342, 1125
BtrI CACGTC 2 cut(s) 1790, 1934
BtsCI GGATG 8 cut(s) 112, 121, 1003, 1078, 1657, 1854, 1995, 2041
BtsI GCAGTG 2 cut(s) 310, 1411
BtsIMutI CAGTG 6 cut(s) 285, 310, 1411, 1458, 1991, 2212
BveI ACCTGC 3 cut(s) 1354, 2101, 2297
Cac8I GCNNGC 6 cut(s) 18, 190, 356, 1411, 2201, 2296
CaiI CAGNNNCTG 4 cut(s) 49, 391, 838, 1416
CciI TCATGA 1 cut(s) 2133
CfoI GCGC 3 cut(s) 315, 2296, 2298
Cfr10I RCCGGY 3 cut(s) 16, 188, 299
Cfr13I GGNCC 5 cut(s) 129, 153, 356, 502, 739
CseI GACGC 4 cut(s) 212, 766, 864, 1691
Csp6I GTAC 6 cut(s) 47, 789, 1020, 1398, 1434, 1555
CspCI CAANNNNNGTGG 2 cut(s) 1225, 1260
CviAII CATG 9 cut(s) 275, 440, 971, 1197, 1325, 1527, 1544, 1822, 2134
CviQI GTAC 6 cut(s) 47, 789, 1020, 1398, 1434, 1555
DdeI CTNAG 9 cut(s) 111, 640, 828, 1230, 1290, 1468, 1983, 2020, 2076
DraI TTTAAA 1 cut(s) 1311
EaeI YGGCCR 3 cut(s) 3, 1193, 2222
Eam1104I CTCTTC 1 cut(s) 1289
EarI CTCTTC 1 cut(s) 1289
EciI GGCGGA 2 cut(s) 35, 2290
Eco130I CCWWGG 6 cut(s) 505, 582, 1196, 1253, 1543, 2087
Eco31I GGTCTC 1 cut(s) 1086
Eco47I GGWCC 3 cut(s) 129, 153, 739
Eco57I CTGAAG 7 cut(s) 53, 92, 492, 766, 1347, 1606, 1889
Eco72I CACGTG 1 cut(s) 1733
Eco88I CYCGRG 1 cut(s) 1697
Eco91I GGTNACC 1 cut(s) 577
EcoNI CCTNNNNNAGG 1 cut(s) 747
EcoO109I RGGNCCY 3 cut(s) 129, 502, 739
EcoO65I GGTNACC 1 cut(s) 577
EcoRII CCWGG 5 cut(s) 42, 540, 1084, 1370, 1586
EcoT14I CCWWGG 6 cut(s) 505, 582, 1196, 1253, 1543, 2087
ErhI CCWWGG 6 cut(s) 505, 582, 1196, 1253, 1543, 2087
Esp3I CGTCTC 1 cut(s) 1777
FaeI CATG 9 cut(s) 278, 443, 974, 1200, 1328, 1530, 1547, 1825, 2137
FaqI GGGAC 3 cut(s) 115, 570, 725
FatI CATG 9 cut(s) 274, 439, 970, 1196, 1324, 1526, 1543, 1821, 2133
FauI CCCGC 2 cut(s) 347, 2178
FauNDI CATATG 1 cut(s) 1797
FbaI TGATCA 2 cut(s) 967, 1267
FblI GTMKAC 3 cut(s) 227, 1539, 2040
FokI GGATG 8 cut(s) 108, 119, 1010, 1085, 1644, 1841, 2002, 2048
FspBI CTAG 5 cut(s) 1017, 1026, 1775, 1886, 2147
GlaI GCGC 3 cut(s) 314, 2295, 2297
GsuI CTGGAG 5 cut(s) 71, 1068, 1081, 1570, 1801
HaeIII GGCC 6 cut(s) 5, 358, 503, 1195, 1252, 2224
HapII CCGG 5 cut(s) 17, 94, 189, 300, 1747
HgaI GACGC 4 cut(s) 212, 766, 864, 1691
HhaI GCGC 3 cut(s) 315, 2296, 2298
Hin1I GRCGYC 1 cut(s) 875
Hin1II CATG 9 cut(s) 278, 443, 974, 1200, 1328, 1530, 1547, 1825, 2137
Hin6I GCGC 3 cut(s) 313, 2294, 2296
HinP1I GCGC 3 cut(s) 313, 2294, 2296
HincII GTYRAC 1 cut(s) 1393
HindII GTYRAC 1 cut(s) 1393
HindIII AAGCTT 2 cut(s) 778, 2249
HinfI GANTC 7 cut(s) 67, 159, 183, 713, 773, 907, 1103
HpaI GTTAAC 1 cut(s) 1393
HpaII CCGG 5 cut(s) 17, 94, 189, 300, 1747
HphI GGTGA 6 cut(s) 379, 571, 1928, 2065, 2125, 2321
Hpy166II GTNNAC 7 cut(s) 228, 370, 1022, 1393, 1456, 1540, 2041
Hpy8I GTNNAC 7 cut(s) 228, 370, 1022, 1393, 1456, 1540, 2041
Hpy99I CGWCG 2 cut(s) 206, 1791
HpyAV CCTTC 5 cut(s) 932, 1115, 1211, 1630, 2288
HpyCH4IV ACGT 4 cut(s) 1566, 1732, 1789, 1933
HpyF3I CTNAG 9 cut(s) 111, 640, 828, 1230, 1290, 1468, 1983, 2020, 2076
HpySE526I ACGT 4 cut(s) 1566, 1732, 1789, 1933
Hsp92I GRCGYC 1 cut(s) 875
Hsp92II CATG 9 cut(s) 278, 443, 974, 1200, 1328, 1530, 1547, 1825, 2137
HspAI GCGC 3 cut(s) 313, 2294, 2296
KflI GGGWCCC 1 cut(s) 739
KroI GCCGGC 2 cut(s) 16, 188
KroNI GCCGGC 2 cut(s) 18, 190
Ksp22I TGATCA 2 cut(s) 967, 1267
KspAI GTTAAC 1 cut(s) 1393
LmnI GCTCC 8 cut(s) 25, 96, 118, 945, 1682, 2065, 2120, 2303
Lsp1109I GCAGC 9 cut(s) 160, 240, 282, 306, 844, 1425, 2183, 2226, 2303
LweI GCATC 5 cut(s) 97, 130, 863, 897, 988
MaeI CTAG 5 cut(s) 1017, 1026, 1775, 1886, 2147
MaeII ACGT 4 cut(s) 1566, 1732, 1789, 1933
MaeIII GTNAC 8 cut(s) 577, 649, 754, 802, 1687, 1714, 1749, 2071
MfeI CAATTG 1 cut(s) 849
MflI RGATCY 7 cut(s) 39, 535, 1367, 1864, 2082, 2151, 2257
MlsI TGGCCA 2 cut(s) 5, 1195
MluCI AATT 9 cut(s) 450, 567, 849, 861, 1003, 1500, 1825, 2187, 2282
MluNI TGGCCA 2 cut(s) 5, 1195
MlyI GAGTC 1 cut(s) 192
MmeI TCCRAC 2 cut(s) 96, 2013
Mox20I TGGCCA 2 cut(s) 5, 1195
MreI CGCCGGCG 2 cut(s) 16, 188
MroNI GCCGGC 2 cut(s) 16, 188
MroXI GAANNNNTTC 2 cut(s) 1950, 2250
MscI TGGCCA 2 cut(s) 5, 1195
MseI TTAA 5 cut(s) 1310, 1386, 1392, 1575, 2186
MslI CAYNNNNRTG 3 cut(s) 1053, 1329, 1662
Msp20I TGGCCA 2 cut(s) 5, 1195
MspA1I CMGCKG 3 cut(s) 151, 253, 1416
MspI CCGG 5 cut(s) 17, 94, 189, 300, 1747
MspR9I CCNGG 5 cut(s) 44, 542, 1086, 1372, 1588
MunI CAATTG 1 cut(s) 849
Mva1269I GAATGC 1 cut(s) 241
MvaI CCWGG 5 cut(s) 44, 542, 1086, 1372, 1588
MvnI CGCG 3 cut(s) 206, 315, 2296
NaeI GCCGGC 2 cut(s) 18, 190
NcoI CCATGG 2 cut(s) 1196, 1543
NdeI CATATG 1 cut(s) 1797
NgoMIV GCCGGC 2 cut(s) 16, 188
NlaIII CATG 9 cut(s) 278, 443, 974, 1200, 1328, 1530, 1547, 1825, 2137
NlaIV GGNNCC 5 cut(s) 131, 740, 741, 1369, 1995
NmuCI GTSAC 4 cut(s) 577, 649, 754, 1687
PagI TCATGA 1 cut(s) 2133
PaqCI CACCTGC 2 cut(s) 2101, 2297
PauI GCGCGC 1 cut(s) 2294
PcsI WCGNNNNNNNCGW 2 cut(s) 122, 1563
PctI GAATGC 1 cut(s) 241
PdiI GCCGGC 2 cut(s) 18, 190
PdmI GAANNNNTTC 2 cut(s) 1950, 2250
PfeI GAWTC 6 cut(s) 67, 159, 713, 773, 907, 1103
PflMI CCANNNNNTGG 1 cut(s) 49
PfoI TCCNGGA 2 cut(s) 1084, 1370
PleI GAGTC 1 cut(s) 191
PmaCI CACGTG 1 cut(s) 1733
PmlI CACGTG 1 cut(s) 1733
PpsI GAGTC 1 cut(s) 191
Ppu21I YACGTR 1 cut(s) 1733
PpuMI RGGWCCY 2 cut(s) 129, 739
Psp5II RGGWCCY 2 cut(s) 129, 739
Psp6I CCWGG 5 cut(s) 42, 540, 1084, 1370, 1586
PspCI CACGTG 1 cut(s) 1733
PspEI GGTNACC 1 cut(s) 577
PspGI CCWGG 5 cut(s) 42, 540, 1084, 1370, 1586
PspN4I GGNNCC 5 cut(s) 131, 740, 741, 1369, 1995
PspPI GGNCC 5 cut(s) 129, 153, 356, 502, 739
PspPPI RGGWCCY 2 cut(s) 129, 739
PstI CTGCAG 2 cut(s) 258, 2265
PstNI CAGNNNCTG 4 cut(s) 49, 391, 838, 1416
PsuI RGATCY 7 cut(s) 39, 535, 1367, 1864, 2082, 2151, 2257
PteI GCGCGC 1 cut(s) 2294
PvuII CAGCTG 1 cut(s) 1416
RsaI GTAC 6 cut(s) 48, 790, 1021, 1399, 1435, 1556
RsaNI GTAC 6 cut(s) 47, 789, 1020, 1398, 1434, 1555
RseI CAYNNNNRTG 3 cut(s) 1053, 1329, 1662
SaqAI TTAA 5 cut(s) 1310, 1386, 1392, 1575, 2186
Sau96I GGNCC 5 cut(s) 129, 153, 356, 502, 739
ScaI AGTACT 1 cut(s) 1435
SchI GAGTC 1 cut(s) 192
ScrFI CCNGG 5 cut(s) 44, 542, 1086, 1372, 1588
SfaNI GCATC 5 cut(s) 97, 130, 863, 897, 988
SfcI CTRYAG 3 cut(s) 254, 2124, 2261
SgrAI CRCCGGYG 2 cut(s) 16, 188
SinI GGWCC 3 cut(s) 129, 153, 739
SmiMI CAYNNNNRTG 3 cut(s) 1053, 1329, 1662
Sse9I AATT 9 cut(s) 450, 567, 849, 861, 1003, 1500, 1825, 2187, 2282
SspI AATATT 1 cut(s) 1598
SspMI CTAG 5 cut(s) 1017, 1026, 1775, 1886, 2147
StyD4I CCNGG 5 cut(s) 42, 540, 1084, 1370, 1586
StyI CCWWGG 6 cut(s) 505, 582, 1196, 1253, 1543, 2087
TaiI ACGT 4 cut(s) 1569, 1735, 1792, 1936
TaqI TCGA 5 cut(s) 164, 1342, 1654, 1861, 2254
TasI AATT 9 cut(s) 450, 567, 849, 861, 1003, 1500, 1825, 2187, 2282
TatI WGTACW 3 cut(s) 1019, 1397, 1433
TfiI GAWTC 6 cut(s) 67, 159, 713, 773, 907, 1103
Tru1I TTAA 5 cut(s) 1310, 1386, 1392, 1575, 2186
Tru9I TTAA 5 cut(s) 1310, 1386, 1392, 1575, 2186
TscAI CASTG 6 cut(s) 292, 310, 1411, 1465, 1991, 2212
TseFI GTSAC 4 cut(s) 577, 649, 754, 1687
TseI GCWGC 9 cut(s) 148, 253, 270, 294, 832, 1413, 2196, 2239, 2291
Tsp45I GTSAC 4 cut(s) 577, 649, 754, 1687
TspGWI ACGGA 1 cut(s) 1572
TspRI CASTG 6 cut(s) 292, 310, 1411, 1465, 1991, 2212
Van91I CCANNNNNTGG 1 cut(s) 49
VpaK11BI GGWCC 3 cut(s) 129, 153, 739
XagI CCTNNNNNAGG 1 cut(s) 747
XapI RAATTY 1 cut(s) 1003
XcmI CCANNNNNNNNNTGG 1 cut(s) 526
XmiI GTMKAC 3 cut(s) 227, 1539, 2040
XmnI GAANNNNTTC 2 cut(s) 1950, 2250
XspI CTAG 5 cut(s) 1017, 1026, 1775, 1886, 2147
ZrmI AGTACT 1 cut(s) 1435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.