Rroxscaffold_5G00367320

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000660
Physical Location & Seq
Forward (+)
49164682 .. 49169830
5149 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00367320.1

Sequence Viewer

Length: 1779 bp
ATGGCCACCAATACTCCATTTTATGATCATGATGGCACTTTCATTGGGGTTATATGTGTTTCATGGGATTTGCGGCATTTTCAAGAAACGAGGGTTCCACTGTTATGTTTGGAACCAGACTCAAGCTTGGGCCGTTGCCCTACCACTAGTGCGGCAACTCAAGTTGTTGTTGATCCTCAGCAAAATGAACTTGCATCAAGAATAACAAATTTGGCATCCAAGGTCTGCAACAAATTCAAGTCAAGAGTTAAGGATGGAAAAGAAAACTTGGATGGCAAAGCTGGGAGTGGAGATAGTCGCTGGTGTGATCATGGTTCAGTTTCTCATCTCTCTGACCATTTGGAGGATGGGTGTTCAATTGGGGCAAGCACACCGAGAGGAGATATACGTCCATCTCCTTTTGGTATGTTTGCAAAAATTAACGACAAGTTTCCAGGTAAACCCTCCGGAGAATATGATAATGAGAGTGAAGGAAAACGCCCAATGCATAAGATAATAACCTCAAAGGCAGAAGCATGGATTGGTAAGAAGGGACTATCGTGGCCATGGAAAGAAAATGAGAAAGAAGGGTCGGTGACTAGGAGCACTCATTTTGTTTGGCCCTGGTTGCATAATGATCAAGGAAAAGATTCAATACCTCAGGAGGGTAATTGTGCTTTAAAACCAGAAAATCAGGTGAATGAAAGTAATCGACCTGCAACTAGTGAAGCTTCAGGAGCCTGGACCTCTTTGTTTAATGTTAACTGCACAAGAAGTGGTAGCAGTTCTGGAAGTACCAACAGTTCTGCCAAAAAGGAGGATGTGGATATTGATTGCTTGGACTATGAAGTCTTATGGGAAGACATGATAATTAGAGAGAGGATTGGGCAAGGTTCATGTGGAACTGTATACCATGGTGTGTGGGATGAATCAGATATTGCTGTTAAGGTATTTTTGAAGCAAGATTATCCAGATGATGTGCTTCTGTCTTTTAGACAAGAGGTATCTCTTATGAAAAGGCTTCGACATCCAAACGTGCTGCTTTTCATGGGAGCAGTGACTTCACCTCAGCGTCTTTGCATTGTAACAGAGTTCCTCCCACGTGGAAGTTTGTTTCAATTACTACAGAAGAACAAGTCAAAACTAGACTGGAGACGACGTGTTCAAATGGCTTTAGATATAGCTCTTGAGGCTTTTTATATCAGACTATCTTCTGGATTGCATCAAATTGTGTTCATCTGGTGTTTGAATAGAGTCTTGGCACGAGGCATGAATTATCTTCATCATTTCAGCCCCCCTATTATTCATCGAGATCTGAAGTCTTCAAATCTCCTAGTTGATAAGAACTGGACTGTCAAGGTTGGTGACTTTGGACTTTCACGTCTTAAGCATGAAAGATTTCTCCATACCAAGACCAGGAAGGGGACGCCTCAATGGATGGCACCAGAAGTTCTGTGTAATGAATCCTCAGATGAGAAGTCTGATGTATACAGCTACGGGGTGATATTGTGGGAGCTCGCTACTGAGAAGATTCCTTGGGATGATCTGAATGCAATGCAGGTGATTGGAGCTGTAGGGTTCAGGAACCAAAGGCTAGAGATCCCAAAGGATGTGGATCCACAATGGGCTTCTATGACTGAGAGATGTTGGCACAGTGATCCAGCTTGCCGGCCAACATTTCATGAATTGCTGGAAGAACTTAAAGCGTTGCAGAGACGGCGTGCTCTCCAGTTCAAAGCAGCGCGTTCTGCCTCTCACTATAGCACCCAAAACGAATTGCAGACCAGCCTGAAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS PAS_9 Pkinase_Tyr

Protein Analysis

592

Amino Acids

67.07

Weight (kDa)

7.27

Isoelectric Point (pI)

42.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 284 - 401 7.5e-22 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 285 - 558 8.6e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 412 - 560 5.6e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1528
AasI GACNNNNNNGTC 2 cut(s) 827, 1359
Acc36I ACCTGC 2 cut(s) 703, 1528
AccB1I GGYRCC 1 cut(s) 1420
AccI GTMKAC 2 cut(s) 888, 1467
AccII CGCG 1 cut(s) 1723
AccIII TCCGGA 1 cut(s) 446
AciI CCGC 2 cut(s) 73, 152
AclWI GGATC 5 cut(s) 167, 1573, 1589, 1602, 1631
AcoI YGGCCR 3 cut(s) 3, 542, 1649
AcsI RAATTY 2 cut(s) 208, 233
AcuI CTGAAG 2 cut(s) 696, 1316
AcvI CACGTG 1 cut(s) 1082
AcyI GRCGYC 1 cut(s) 1406
AfaI GTAC 1 cut(s) 775
AfiI CCNNNNNNNGG 4 cut(s) 343, 644, 1395, 1401
AflII CTTAAG 1 cut(s) 1364
AflIII ACRYGT 1 cut(s) 1138
AhlI ACTAGT 2 cut(s) 146, 701
AjiI CACGTC 2 cut(s) 1139, 1361
AjnI CCWGG 4 cut(s) 433, 602, 719, 1394
AjuI GAANNNNNNNTTGG 4 cut(s) 504, 536, 1220, 1252
AluBI AGCT 8 cut(s) 126, 281, 710, 1163, 1473, 1495, 1550, 1643
AluI AGCT 8 cut(s) 126, 281, 710, 1163, 1473, 1495, 1550, 1643
Alw21I GWGCWC 3 cut(s) 587, 1497, 1705
Alw26I GTCTC 2 cut(s) 1126, 1687
AlwI GGATC 5 cut(s) 167, 1573, 1589, 1602, 1631
Aor13HI TCCGGA 1 cut(s) 446
AoxI GGCC 5 cut(s) 3, 130, 542, 599, 1649
ApeKI GCWGC 2 cut(s) 1018, 1718
ApoI RAATTY 2 cut(s) 208, 233
Asp700I GAANNNNTTC 2 cut(s) 628, 1377
AspLEI GCGC 1 cut(s) 1723
AspS9I GGNCC 3 cut(s) 130, 600, 723
AsuHPI GGTGA 6 cut(s) 586, 688, 1035, 1355, 1492, 1552
AvaII GGWCC 1 cut(s) 723
AxyI CCTNAGG 1 cut(s) 639
BalI TGGCCA 2 cut(s) 5, 544
BamHI GGATCC 1 cut(s) 1594
BanI GGYRCC 1 cut(s) 1420
BanII GRGCYC 1 cut(s) 1497
BauI CACGAG 1 cut(s) 1242
BbrPI CACGTG 1 cut(s) 1082
BbsI GAAGAC 2 cut(s) 846, 1293
Bbv12I GWGCWC 3 cut(s) 587, 1497, 1705
BbvCI CCTCAGC 2 cut(s) 177, 1047
BbvI GCAGC 2 cut(s) 1005, 1730
BccI CCATC 6 cut(s) 26, 248, 266, 341, 400, 1411
BceAI ACGGC 2 cut(s) 117, 1712
BciT130I CCWGG 4 cut(s) 435, 604, 721, 1396
BclI TGATCA 3 cut(s) 25, 307, 616
BcoDI GTCTC 2 cut(s) 1126, 1687
BcuI ACTAGT 2 cut(s) 146, 701
BfaI CTAG 6 cut(s) 147, 579, 702, 1124, 1313, 1574
BfmI CTRYAG 3 cut(s) 1103, 1551, 1738
BfrI CTTAAG 1 cut(s) 1364
BfuAI ACCTGC 2 cut(s) 703, 1528
BglII AGATCT 1 cut(s) 1291
BisI GCNGC 4 cut(s) 74, 153, 1019, 1719
BlsI GCNGC 4 cut(s) 75, 154, 1020, 1720
Bme1390I CCNGG 4 cut(s) 435, 604, 721, 1396
Bme18I GGWCC 1 cut(s) 723
BmgBI CACGTC 2 cut(s) 1139, 1361
BmgT120I GGNCC 3 cut(s) 130, 600, 723
BmiI GGNNCC 6 cut(s) 96, 114, 718, 1422, 1565, 1596
BmrFI CCNGG 4 cut(s) 435, 604, 721, 1396
BmsI GCATC 3 cut(s) 203, 224, 1210
BpiI GAAGAC 2 cut(s) 846, 1293
BpmI CTGGAG 2 cut(s) 1150, 1691
Bpu10I CCTNAGC 2 cut(s) 177, 1047
BpuEI CTTGAG 3 cut(s) 106, 144, 1187
BsaAI YACGTR 1 cut(s) 1082
BsaBI GATNNNNATC 1 cut(s) 1593
BsaHI GRCGYC 1 cut(s) 1406
BsaJI CCNNGG 5 cut(s) 219, 545, 602, 892, 1514
BsaWI WCCGGW 1 cut(s) 446
BsaXI ACNNNNNCTCC 5 cut(s) 28, 335, 365, 1123, 1153
Bsc4I CCNNNNNNNGG 4 cut(s) 343, 644, 1395, 1401
Bse118I RCCGGY 1 cut(s) 1647
Bse1I ACTGG 3 cut(s) 1133, 1331, 1708
Bse21I CCTNAGG 1 cut(s) 639
Bse3DI GCAATG 1 cut(s) 1539
Bse8I GATNNNNATC 1 cut(s) 1593
BseAI TCCGGA 1 cut(s) 446
BseBI CCWGG 4 cut(s) 435, 604, 721, 1396
BseDI CCNNGG 5 cut(s) 219, 545, 602, 892, 1514
BseJI GATNNNNATC 1 cut(s) 1593
BseLI CCNNNNNNNGG 4 cut(s) 343, 644, 1395, 1401
BseMI GCAATG 1 cut(s) 1539
BseMII CTCAG 6 cut(s) 191, 653, 1061, 1461, 1494, 1608
BseNI ACTGG 3 cut(s) 1133, 1331, 1708
BseRI GAGGAG 1 cut(s) 393
BseXI GCAGC 2 cut(s) 1005, 1730
BseYI CCCAGC 1 cut(s) 281
BsgI GTGCAG 1 cut(s) 730
Bsh1236I CGCG 1 cut(s) 1723
BshFI GGCC 5 cut(s) 5, 132, 544, 601, 1651
BshNI GGYRCC 1 cut(s) 1420
BsiHKAI GWGCWC 3 cut(s) 587, 1497, 1705
BsiSI CCGG 2 cut(s) 447, 1648
BslFI GGGAC 2 cut(s) 546, 1417
BslI CCNNNNNNNGG 4 cut(s) 343, 644, 1395, 1401
BsmAI GTCTC 2 cut(s) 1126, 1687
BsmBI CGTCTC 2 cut(s) 1126, 1687
BsmFI GGGAC 2 cut(s) 546, 1417
BsmI GAATGC 1 cut(s) 1534
BsnI GGCC 5 cut(s) 5, 132, 544, 601, 1651
Bsp1286I GDGCHC 3 cut(s) 587, 1497, 1705
Bsp13I TCCGGA 1 cut(s) 446
Bsp143I GATC 9 cut(s) 25, 172, 307, 616, 1291, 1522, 1578, 1594, 1636
Bsp19I CCATGG 2 cut(s) 545, 892
BspACI CCGC 2 cut(s) 73, 152
BspANI GGCC 5 cut(s) 5, 132, 544, 601, 1651
BspCNI CTCAG 6 cut(s) 190, 652, 1060, 1460, 1495, 1609
BspEI TCCGGA 1 cut(s) 446
BspFNI CGCG 1 cut(s) 1723
BspHI TCATGA 2 cut(s) 28, 1660
BspLI GGNNCC 6 cut(s) 96, 114, 718, 1422, 1565, 1596
BspMI ACCTGC 2 cut(s) 703, 1528
BspPI GGATC 5 cut(s) 167, 1573, 1589, 1602, 1631
BspT107I GGYRCC 1 cut(s) 1420
BspTI CTTAAG 1 cut(s) 1364
BsrDI GCAATG 1 cut(s) 1539
BsrFI RCCGGY 1 cut(s) 1647
BsrI ACTGG 3 cut(s) 1133, 1331, 1708
BssAI RCCGGY 1 cut(s) 1647
BssECI CCNNGG 5 cut(s) 219, 545, 602, 892, 1514
BssMI GATC 9 cut(s) 25, 172, 307, 616, 1291, 1522, 1578, 1594, 1636
BssNAI GTATAC 2 cut(s) 889, 1468
BssNI GRCGYC 1 cut(s) 1406
BssSI CACGAG 1 cut(s) 1242
BssT1I CCWWGG 4 cut(s) 219, 545, 892, 1514
Bst1107I GTATAC 2 cut(s) 889, 1468
Bst2BI CACGAG 1 cut(s) 1242
Bst2UI CCWGG 4 cut(s) 435, 604, 721, 1396
Bst4CI ACNGT 5 cut(s) 102, 782, 886, 1333, 1634
BstACI GRCGYC 1 cut(s) 1406
BstAFI CTTAAG 1 cut(s) 1364
BstBAI YACGTR 1 cut(s) 1082
BstC8I GCNNGC 5 cut(s) 367, 1497, 1645, 1649, 1701
BstDEI CTNAG 6 cut(s) 177, 639, 1047, 1447, 1503, 1617
BstDSI CCRYGG 2 cut(s) 545, 892
BstFNI CGCG 1 cut(s) 1723
BstHHI GCGC 1 cut(s) 1723
BstKTI GATC 9 cut(s) 28, 175, 310, 619, 1294, 1525, 1581, 1597, 1639
BstMAI GTCTC 2 cut(s) 1126, 1687
BstMBI GATC 9 cut(s) 25, 172, 307, 616, 1291, 1522, 1578, 1594, 1636
BstMWI GCNNNNNNNGC 5 cut(s) 607, 716, 1169, 1696, 1727
BstNI CCWGG 4 cut(s) 435, 604, 721, 1396
BstSCI CCNGG 4 cut(s) 433, 602, 719, 1394
BstSFI CTRYAG 3 cut(s) 1103, 1551, 1738
BstUI CGCG 1 cut(s) 1723
BstV1I GCAGC 2 cut(s) 1005, 1730
BstV2I GAAGAC 2 cut(s) 846, 1293
BstX2I RGATCY 3 cut(s) 1291, 1578, 1594
BstYI RGATCY 3 cut(s) 1291, 1578, 1594
BstZ17I GTATAC 2 cut(s) 889, 1468
Bsu36I CCTNAGG 1 cut(s) 639
BsuRI GGCC 5 cut(s) 5, 132, 544, 601, 1651
BtgI CCRYGG 2 cut(s) 545, 892
BtrI CACGTC 2 cut(s) 1139, 1361
BtsI GCAGTG 1 cut(s) 1041
BtsIMutI CAGTG 3 cut(s) 98, 1041, 1639
BveI ACCTGC 2 cut(s) 703, 1528
Cac8I GCNNGC 5 cut(s) 367, 1497, 1645, 1649, 1701
CciI TCATGA 2 cut(s) 28, 1660
CfoI GCGC 1 cut(s) 1723
Cfr10I RCCGGY 1 cut(s) 1647
Cfr13I GGNCC 3 cut(s) 130, 600, 723
CseI GACGC 2 cut(s) 1040, 1414
Csp6I GTAC 1 cut(s) 774
CspCI CAANNNNNGTGG 2 cut(s) 1572, 1607
CviQI GTAC 1 cut(s) 774
DdeI CTNAG 6 cut(s) 177, 639, 1047, 1447, 1503, 1617
DpnI GATC 9 cut(s) 27, 174, 309, 618, 1293, 1524, 1580, 1596, 1638
DpnII GATC 9 cut(s) 25, 172, 307, 616, 1291, 1522, 1578, 1594, 1636
DraI TTTAAA 1 cut(s) 660
DrdI GACNNNNNNGTC 2 cut(s) 827, 1359
DseDI GACNNNNNNGTC 2 cut(s) 827, 1359
EaeI YGGCCR 3 cut(s) 3, 542, 1649
Ecl136II GAGCTC 1 cut(s) 1495
Eco130I CCWWGG 4 cut(s) 219, 545, 892, 1514
Eco24I GRGCYC 1 cut(s) 1497
Eco47I GGWCC 1 cut(s) 723
Eco53kI GAGCTC 1 cut(s) 1495
Eco57I CTGAAG 2 cut(s) 696, 1316
Eco72I CACGTG 1 cut(s) 1082
Eco81I CCTNAGG 1 cut(s) 639
EcoICRI GAGCTC 1 cut(s) 1495
EcoRII CCWGG 4 cut(s) 433, 602, 719, 1394
EcoT14I CCWWGG 4 cut(s) 219, 545, 892, 1514
EcoT22I ATGCAT 1 cut(s) 489
EcoT38I GRGCYC 1 cut(s) 1497
ErhI CCWWGG 4 cut(s) 219, 545, 892, 1514
Esp3I CGTCTC 2 cut(s) 1126, 1687
FaqI GGGAC 2 cut(s) 546, 1417
FbaI TGATCA 3 cut(s) 25, 307, 616
FblI GTMKAC 2 cut(s) 888, 1467
Fnu4HI GCNGC 4 cut(s) 74, 153, 1019, 1719
FriOI GRGCYC 1 cut(s) 1497
Fsp4HI GCNGC 4 cut(s) 74, 153, 1019, 1719
FspBI CTAG 6 cut(s) 147, 579, 702, 1124, 1313, 1574
GlaI GCGC 1 cut(s) 1722
GluI GCNGC 4 cut(s) 74, 153, 1019, 1719
GsaI CCCAGC 1 cut(s) 285
GsuI CTGGAG 2 cut(s) 1150, 1691
HaeIII GGCC 5 cut(s) 5, 132, 544, 601, 1651
HapII CCGG 2 cut(s) 447, 1648
HgaI GACGC 2 cut(s) 1040, 1414
HhaI GCGC 1 cut(s) 1723
Hin1I GRCGYC 1 cut(s) 1406
Hin6I GCGC 1 cut(s) 1721
HinP1I GCGC 1 cut(s) 1721
HincII GTYRAC 1 cut(s) 742
HindII GTYRAC 1 cut(s) 742
HindIII AAGCTT 2 cut(s) 124, 708
HinfI GANTC 6 cut(s) 119, 629, 908, 1233, 1442, 1510
HpaI GTTAAC 1 cut(s) 742
HpaII CCGG 2 cut(s) 447, 1648
HphI GGTGA 6 cut(s) 586, 688, 1035, 1355, 1492, 1552
Hpy166II GTNNAC 4 cut(s) 440, 742, 889, 1468
Hpy188I TCNGA 7 cut(s) 334, 913, 1184, 1296, 1450, 1462, 1527
Hpy8I GTNNAC 4 cut(s) 440, 742, 889, 1468
Hpy99I CGWCG 1 cut(s) 1140
HpyAV CCTTC 4 cut(s) 464, 523, 560, 1393
HpyCH4III ACNGT 5 cut(s) 102, 782, 886, 1333, 1634
HpyCH4IV ACGT 5 cut(s) 388, 1014, 1081, 1138, 1360
HpyF10VI GCNNNNNNNGC 5 cut(s) 607, 716, 1169, 1696, 1727
HpyF3I CTNAG 6 cut(s) 177, 639, 1047, 1447, 1503, 1617
HpySE526I ACGT 5 cut(s) 388, 1014, 1081, 1138, 1360
Hsp92I GRCGYC 1 cut(s) 1406
HspAI GCGC 1 cut(s) 1721
Kpn2I TCCGGA 1 cut(s) 446
KroI GCCGGC 1 cut(s) 1647
KroNI GCCGGC 1 cut(s) 1649
Ksp22I TGATCA 3 cut(s) 25, 307, 616
KspAI GTTAAC 1 cut(s) 742
Kzo9I GATC 9 cut(s) 25, 172, 307, 616, 1291, 1522, 1578, 1594, 1636
LmnI GCTCC 5 cut(s) 582, 716, 1031, 1492, 1547
Lsp1109I GCAGC 2 cut(s) 1005, 1730
LweI GCATC 3 cut(s) 203, 224, 1210
MaeI CTAG 6 cut(s) 147, 579, 702, 1124, 1313, 1574
MaeII ACGT 5 cut(s) 388, 1014, 1081, 1138, 1360
MaeIII GTNAC 4 cut(s) 574, 1036, 1063, 1343
MalI GATC 9 cut(s) 27, 174, 309, 618, 1293, 1524, 1580, 1596, 1638
MboI GATC 9 cut(s) 25, 172, 307, 616, 1291, 1522, 1578, 1594, 1636
MboII GAAGA 7 cut(s) 851, 1120, 1182, 1250, 1293, 1519, 1685
MfeI CAATTG 1 cut(s) 357
MflI RGATCY 3 cut(s) 1291, 1578, 1594
MhlI GDGCHC 3 cut(s) 587, 1497, 1705
MlsI TGGCCA 2 cut(s) 5, 544
MluNI TGGCCA 2 cut(s) 5, 544
MlyI GAGTC 2 cut(s) 113, 1242
Mox20I TGGCCA 2 cut(s) 5, 544
Mph1103I ATGCAT 1 cut(s) 489
MroI TCCGGA 1 cut(s) 446
MroNI GCCGGC 1 cut(s) 1647
MroXI GAANNNNTTC 2 cut(s) 628, 1377
MscI TGGCCA 2 cut(s) 5, 544
MseI TTAA 8 cut(s) 249, 420, 659, 735, 741, 924, 1365, 1680
MslI CAYNNNNRTG 1 cut(s) 103
Msp20I TGGCCA 2 cut(s) 5, 544
MspCI CTTAAG 1 cut(s) 1364
MspI CCGG 2 cut(s) 447, 1648
MspR9I CCNGG 4 cut(s) 435, 604, 721, 1396
MunI CAATTG 1 cut(s) 357
Mva1269I GAATGC 1 cut(s) 1534
MvaI CCWGG 4 cut(s) 435, 604, 721, 1396
MvnI CGCG 1 cut(s) 1723
MwoI GCNNNNNNNGC 5 cut(s) 607, 716, 1169, 1696, 1727
NaeI GCCGGC 1 cut(s) 1649
NcoI CCATGG 2 cut(s) 545, 892
NdeII GATC 9 cut(s) 25, 172, 307, 616, 1291, 1522, 1578, 1594, 1636
NgoMIV GCCGGC 1 cut(s) 1647
NlaIV GGNNCC 6 cut(s) 96, 114, 718, 1422, 1565, 1596
NmuCI GTSAC 3 cut(s) 574, 1036, 1343
NsiI ATGCAT 1 cut(s) 489
PagI TCATGA 2 cut(s) 28, 1660
PaqCI CACCTGC 1 cut(s) 1528
PctI GAATGC 1 cut(s) 1534
PdiI GCCGGC 1 cut(s) 1649
PdmI GAANNNNTTC 2 cut(s) 628, 1377
PfeI GAWTC 4 cut(s) 629, 908, 1442, 1510
PkrI GCNGC 4 cut(s) 75, 154, 1020, 1720
PleI GAGTC 2 cut(s) 113, 1241
PmaCI CACGTG 1 cut(s) 1082
PmlI CACGTG 1 cut(s) 1082
PpsI GAGTC 2 cut(s) 113, 1241
Ppu21I YACGTR 1 cut(s) 1082
Psp124BI GAGCTC 1 cut(s) 1497
Psp6I CCWGG 4 cut(s) 433, 602, 719, 1394
PspCI CACGTG 1 cut(s) 1082
PspFI CCCAGC 1 cut(s) 281
PspGI CCWGG 4 cut(s) 433, 602, 719, 1394
PspN4I GGNNCC 6 cut(s) 96, 114, 718, 1422, 1565, 1596
PspPI GGNCC 3 cut(s) 130, 600, 723
PsrI GAACNNNNNNTAC 2 cut(s) 766, 798
PsuI RGATCY 3 cut(s) 1291, 1578, 1594
RsaI GTAC 1 cut(s) 775
RsaNI GTAC 1 cut(s) 774
RseI CAYNNNNRTG 1 cut(s) 103
SacI GAGCTC 1 cut(s) 1497
SaqAI TTAA 8 cut(s) 249, 420, 659, 735, 741, 924, 1365, 1680
SatI GCNGC 4 cut(s) 74, 153, 1019, 1719
Sau3AI GATC 9 cut(s) 25, 172, 307, 616, 1291, 1522, 1578, 1594, 1636
Sau96I GGNCC 3 cut(s) 130, 600, 723
SchI GAGTC 2 cut(s) 113, 1242
ScrFI CCNGG 4 cut(s) 435, 604, 721, 1396
SduI GDGCHC 3 cut(s) 587, 1497, 1705
SfaNI GCATC 3 cut(s) 203, 224, 1210
SfcI CTRYAG 3 cut(s) 1103, 1551, 1738
SinI GGWCC 1 cut(s) 723
SmiMI CAYNNNNRTG 1 cut(s) 103
SmlI CTYRAG 4 cut(s) 121, 159, 1166, 1364
SmoI CTYRAG 4 cut(s) 121, 159, 1166, 1364
SpeI ACTAGT 2 cut(s) 146, 701
SsiI CCGC 2 cut(s) 73, 152
SspMI CTAG 6 cut(s) 147, 579, 702, 1124, 1313, 1574
SstI GAGCTC 1 cut(s) 1497
StyD4I CCNGG 4 cut(s) 433, 602, 719, 1394
StyI CCWWGG 4 cut(s) 219, 545, 892, 1514
TaaI ACNGT 5 cut(s) 102, 782, 886, 1333, 1634
TaiI ACGT 5 cut(s) 391, 1017, 1084, 1141, 1363
TaqI TCGA 3 cut(s) 691, 1003, 1288
TauI GCSGC 2 cut(s) 76, 155
TfiI GAWTC 4 cut(s) 629, 908, 1442, 1510
Tru1I TTAA 8 cut(s) 249, 420, 659, 735, 741, 924, 1365, 1680
Tru9I TTAA 8 cut(s) 249, 420, 659, 735, 741, 924, 1365, 1680
TscAI CASTG 3 cut(s) 105, 1041, 1639
TseFI GTSAC 3 cut(s) 574, 1036, 1343
TseI GCWGC 2 cut(s) 1018, 1718
Tsp45I GTSAC 3 cut(s) 574, 1036, 1343
TspRI CASTG 3 cut(s) 105, 1041, 1639
Vha464I CTTAAG 1 cut(s) 1364
VpaK11BI GGWCC 1 cut(s) 723
XapI RAATTY 2 cut(s) 208, 233
XcmI CCANNNNNNNNNTGG 1 cut(s) 344
XmiI GTMKAC 2 cut(s) 888, 1467
XmnI GAANNNNTTC 2 cut(s) 628, 1377
XspI CTAG 6 cut(s) 147, 579, 702, 1124, 1313, 1574
Zsp2I ATGCAT 1 cut(s) 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.