Rh4DG261100

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000660
Physical Location & Seq
Forward (+)
47868058 .. 47873156
5099 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG261100.1

Sequence Viewer

Length: 1155 bp
ATGGTCGAAAGTGATCTCGCCCACCTCAAGCAAGAAATGTCCCAGCTCACCAGCCACCGCCGAATCGCCAGCTCCGGTACTCCGCAGCCCAAAACGAGCGGCAGTGCCAGTCGGCTGCCGTCGACTTTTAACTTGAGTGATGCTCAGTATCTCAACATATTGCAGTCGATTGGGCAGTCTGTGCATGCAATGGATCGTAGTGGTCGTATAATCTACTGGAACAGAAGTGCTGAAAAACTGTATGGTTATTCTTCGGAAGAGGCCCTTGGAAAGGTTTCCATTAAGCTCATAGTAGATTCCCGGGACTTTGGTGTAGCATATAATATAGTCCACCGTGTCATCCACGGGGAGAGTTGGACTGGGCAGTTCCCTGTAAAGCACAAATCGGGGGCGAGGTTTTCAGTCATTACAACAAATACTCCATTCTATGATGATGCTGGCAGTTTAATTGGGGTTGTTGCTACATCAACTGATTCTCAGCCCTTTTATGAAATGAGGGTTCCAATGTCAGCTGAGAAGCAACCACAACAAGATCTAAGCTTCAGCAGTCGTGAGGCTAGTGTTATAACTAAATTCGGTGCTGATCCTCAGCAGCCTCTGCCAAAAGCAGTTTTGTCAAAACTAACAGACTTGGCATGGGGCATGAATTATCTTCACCATTTCAACCCACCAATCATTCATCGAGACTTAAAGTCTTCAAATCTCCTAGTTGATAAGAACTGGACTGTCAAGGTTGGCGATTTTGGTCTTTCACGTTTTAAGCATGAAACTTTTCTTATAAACAAGAGTGGCAGGGGAACGCCTCAATGGATGTCACCAGAATTTCTACGTGGTGAACCCTCGGATGAGAAGTCTGACATATATAGCTACGGGGTGATATTGTGGGAGCTTGCTACCGAGAAGATTCCTTGGGATAATCTAAACCCAATGCAGGTGATTGGAGCTGTTGGATTCATGAACCAAAGACTGGAGATCCCAAAAGATGTGGACCCAAAGTGGGCTTCTATAATTGAGAGTTGCTGGAGCAGTGATCCAGCTAGCCGGCCAACATTTCTAGAACTGCTCGAAAAGCTTAGAGTGTTGCATAGACAATATAATCTTCAGTTCAAAGCAGCACGTTCCACCTCTAATGAGAGCACCCAAAAAGAATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS PAS_9 Pkinase_Tyr

Protein Analysis

384

Amino Acids

43.27

Weight (kDa)

8.42

Isoelectric Point (pI)

47.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS PF00989 49 - 155 2.9e-11 PAS fold
PAS_4 PF08448 55 - 159 2.3e-09 PAS fold
PAS_9 PF13426 65 - 158 2.6e-08 PAS domain
PAS_3 PF08447 70 - 155 1e-08 PAS fold
PK_Tyr_Ser-Thr PF07714 201 - 358 1.9e-38 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 209 - 356 4.3e-32 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 566, 779
AarI CACCTGC 1 cut(s) 922
Acc36I ACCTGC 1 cut(s) 922
AccB7I CCANNNNNTGG 1 cut(s) 967
AccBSI CCGCTC 1 cut(s) 99
AccI GTMKAC 1 cut(s) 122
AciI CCGC 3 cut(s) 58, 83, 99
AclWI GGATC 4 cut(s) 201, 578, 967, 1025
AcoI YGGCCR 1 cut(s) 1043
AcsI RAATTY 2 cut(s) 572, 821
AcuI CTGAAG 2 cut(s) 526, 1085
AfaI GTAC 1 cut(s) 79
AfiI CCNNNNNNNGG 4 cut(s) 271, 931, 967, 997
AgsI TTSAA 3 cut(s) 664, 699, 1108
AhdI GACNNNNNGTC 1 cut(s) 691
AjuI GAANNNNNNNTTGG 4 cut(s) 249, 281, 985, 1017
Alw21I GWGCWC 1 cut(s) 1139
Alw26I GTCTC 1 cut(s) 678
AlwI GGATC 4 cut(s) 201, 578, 967, 1025
AlwNI CAGNNNCTG 1 cut(s) 598
Ama87I CYCGRG 1 cut(s) 300
AoxI GGCC 2 cut(s) 261, 1043
ApeKI GCWGC 4 cut(s) 85, 115, 592, 1112
ApoI RAATTY 2 cut(s) 572, 821
Asp700I GAANNNNTTC 2 cut(s) 274, 771
AspS9I GGNCC 2 cut(s) 262, 988
AsuC2I CCSGG 2 cut(s) 301, 302
AsuHPI GGTGA 6 cut(s) 40, 647, 807, 845, 886, 946
AsuNHI GCTAGC 1 cut(s) 1037
AvaI CYCGRG 1 cut(s) 300
AvaII GGWCC 1 cut(s) 988
BbsI GAAGAC 1 cut(s) 687
Bbv12I GWGCWC 1 cut(s) 1139
BbvCI CCTCAGC 1 cut(s) 588
BbvI GCAGC 4 cut(s) 97, 102, 604, 1124
BceAI ACGGC 1 cut(s) 103
BcnI CCSGG 2 cut(s) 301, 302
BcoDI GTCTC 1 cut(s) 678
BfaI CTAG 4 cut(s) 558, 707, 1038, 1055
BfuAI ACCTGC 1 cut(s) 922
BglII AGATCT 1 cut(s) 532
BisI GCNGC 5 cut(s) 86, 100, 116, 593, 1113
BlsI GCNGC 5 cut(s) 87, 101, 117, 594, 1114
Bme1390I CCNGG 2 cut(s) 301, 302
Bme18I GGWCC 1 cut(s) 988
BmeRI GACNNNNNGTC 1 cut(s) 691
BmeT110I CYCGRG 1 cut(s) 300
BmgT120I GGNCC 2 cut(s) 262, 988
BmiI GGNNCC 2 cut(s) 501, 990
BmrFI CCNGG 2 cut(s) 301, 302
BmrI ACTGGG 1 cut(s) 369
BmsI GCATC 2 cut(s) 130, 424
BmtI GCTAGC 1 cut(s) 1041
BmuI ACTGGG 1 cut(s) 369
BpiI GAAGAC 1 cut(s) 687
BplI GAGNNNNNCTC 2 cut(s) 127, 159
BpmI CTGGAG 2 cut(s) 989, 1042
Bpu10I CCTNAGC 1 cut(s) 588
BpuEI CTTGAG 2 cut(s) 11, 154
BpuMI CCSGG 2 cut(s) 301, 302
BsaAI YACGTR 1 cut(s) 830
BsaJI CCNNGG 5 cut(s) 265, 300, 343, 840, 908
BsaWI WCCGGW 1 cut(s) 74
BsaXI ACNNNNNCTCC 2 cut(s) 403, 433
Bsc4I CCNNNNNNNGG 4 cut(s) 271, 931, 967, 997
Bse118I RCCGGY 1 cut(s) 1041
Bse1I ACTGG 5 cut(s) 108, 221, 364, 725, 972
Bse3DI GCAATG 1 cut(s) 195
BseDI CCNNGG 5 cut(s) 265, 300, 343, 840, 908
BseGI GGATG 3 cut(s) 339, 816, 850
BseLI CCNNNNNNNGG 4 cut(s) 271, 931, 967, 997
BseMI GCAATG 1 cut(s) 195
BseMII CTCAG 4 cut(s) 158, 491, 504, 602
BseNI ACTGG 5 cut(s) 108, 221, 364, 725, 972
BseXI GCAGC 4 cut(s) 97, 102, 604, 1124
BseYI CCCAGC 1 cut(s) 42
BshFI GGCC 2 cut(s) 263, 1045
BsiHKAI GWGCWC 1 cut(s) 1139
BsiHKCI CYCGRG 1 cut(s) 300
BsiSI CCGG 3 cut(s) 75, 301, 1042
BslFI GGGAC 2 cut(s) 25, 317
BslI CCNNNNNNNGG 4 cut(s) 271, 931, 967, 997
BsmAI GTCTC 1 cut(s) 678
BsmFI GGGAC 2 cut(s) 25, 317
BsnI GGCC 2 cut(s) 263, 1045
BsoBI CYCGRG 1 cut(s) 300
Bsp1286I GDGCHC 1 cut(s) 1139
Bsp143I GATC 6 cut(s) 13, 193, 532, 583, 972, 1030
BspACI CCGC 3 cut(s) 58, 83, 99
BspANI GGCC 2 cut(s) 263, 1045
BspCNI CTCAG 4 cut(s) 157, 490, 505, 601
BspHI TCATGA 1 cut(s) 954
BspLI GGNNCC 2 cut(s) 501, 990
BspMI ACCTGC 1 cut(s) 922
BspOI GCTAGC 1 cut(s) 1041
BspPI GGATC 4 cut(s) 201, 578, 967, 1025
BsrBI CCGCTC 1 cut(s) 99
BsrDI GCAATG 1 cut(s) 195
BsrFI RCCGGY 1 cut(s) 1041
BsrI ACTGG 5 cut(s) 108, 221, 364, 725, 972
BssAI RCCGGY 1 cut(s) 1041
BssECI CCNNGG 5 cut(s) 265, 300, 343, 840, 908
BssMI GATC 6 cut(s) 13, 193, 532, 583, 972, 1030
BssT1I CCWWGG 2 cut(s) 265, 908
Bst4CI ACNGT 3 cut(s) 240, 335, 727
Bst6I CTCTTC 1 cut(s) 252
BstAPI GCANNNNNTGC 2 cut(s) 181, 598
BstBAI YACGTR 1 cut(s) 830
BstC8I GCNNGC 6 cut(s) 70, 186, 439, 891, 1039, 1043
BstDEI CTNAG 6 cut(s) 144, 477, 513, 536, 588, 1073
BstDSI CCRYGG 1 cut(s) 343
BstENI CCTNNNNNAGG 1 cut(s) 269
BstF5I GGATG 3 cut(s) 339, 816, 850
BstKTI GATC 6 cut(s) 16, 196, 535, 586, 975, 1033
BstMAI GTCTC 1 cut(s) 678
BstMBI GATC 6 cut(s) 13, 193, 532, 583, 972, 1030
BstMWI GCNNNNNNNGC 3 cut(s) 181, 598, 1069
BstNSI RCATGY 1 cut(s) 188
BstSCI CCNGG 2 cut(s) 299, 300
BstV1I GCAGC 4 cut(s) 97, 102, 604, 1124
BstV2I GAAGAC 1 cut(s) 687
BstX2I RGATCY 2 cut(s) 532, 972
BstYI RGATCY 2 cut(s) 532, 972
BsuRI GGCC 2 cut(s) 263, 1045
BtgI CCRYGG 1 cut(s) 343
BtsCI GGATG 3 cut(s) 339, 816, 850
BtsI GCAGTG 2 cut(s) 109, 1033
BtsIMutI CAGTG 2 cut(s) 109, 1033
BveI ACCTGC 1 cut(s) 922
Cac8I GCNNGC 6 cut(s) 70, 186, 439, 891, 1039, 1043
CaiI CAGNNNCTG 1 cut(s) 598
CciI TCATGA 1 cut(s) 954
Cfr10I RCCGGY 1 cut(s) 1041
Cfr13I GGNCC 2 cut(s) 262, 988
Cfr9I CCCGGG 1 cut(s) 300
Csp6I GTAC 1 cut(s) 78
CspCI CAANNNNNGTGG 2 cut(s) 966, 1001
CviAII CATG 5 cut(s) 185, 636, 643, 764, 955
CviQI GTAC 1 cut(s) 78
DdeI CTNAG 6 cut(s) 144, 477, 513, 536, 588, 1073
DpnI GATC 6 cut(s) 15, 195, 534, 585, 974, 1032
DpnII GATC 6 cut(s) 13, 193, 532, 583, 972, 1030
DriI GACNNNNNGTC 1 cut(s) 691
EaeI YGGCCR 1 cut(s) 1043
Eam1104I CTCTTC 1 cut(s) 252
Eam1105I GACNNNNNGTC 1 cut(s) 691
EarI CTCTTC 1 cut(s) 252
Eco130I CCWWGG 2 cut(s) 265, 908
Eco47I GGWCC 1 cut(s) 988
Eco57I CTGAAG 2 cut(s) 526, 1085
Eco88I CYCGRG 1 cut(s) 300
EcoNI CCTNNNNNAGG 1 cut(s) 269
EcoO109I RGGNCCY 1 cut(s) 262
EcoT14I CCWWGG 2 cut(s) 265, 908
ErhI CCWWGG 2 cut(s) 265, 908
FaeI CATG 5 cut(s) 188, 639, 646, 767, 958
FalI AAGNNNNNCTT 2 cut(s) 249, 281
FaqI GGGAC 2 cut(s) 25, 317
FatI CATG 5 cut(s) 184, 635, 642, 763, 954
FblI GTMKAC 1 cut(s) 122
Fnu4HI GCNGC 5 cut(s) 86, 100, 116, 593, 1113
FokI GGATG 3 cut(s) 326, 823, 857
Fsp4HI GCNGC 5 cut(s) 86, 100, 116, 593, 1113
FspBI CTAG 4 cut(s) 558, 707, 1038, 1055
GluI GCNGC 5 cut(s) 86, 100, 116, 593, 1113
GsaI CCCAGC 1 cut(s) 46
GsuI CTGGAG 2 cut(s) 989, 1042
HaeIII GGCC 2 cut(s) 263, 1045
HapII CCGG 3 cut(s) 75, 301, 1042
Hin1II CATG 5 cut(s) 188, 639, 646, 767, 958
HincII GTYRAC 1 cut(s) 123
HindII GTYRAC 1 cut(s) 123
HindIII AAGCTT 2 cut(s) 538, 1070
HinfI GANTC 5 cut(s) 63, 296, 473, 904, 951
HpaII CCGG 3 cut(s) 75, 301, 1042
HphI GGTGA 6 cut(s) 40, 647, 807, 845, 886, 946
Hpy166II GTNNAC 4 cut(s) 123, 331, 836, 988
Hpy188I TCNGA 3 cut(s) 256, 844, 856
Hpy188III TCNNGA 4 cut(s) 551, 683, 955, 1055
Hpy8I GTNNAC 4 cut(s) 123, 331, 836, 988
Hpy99I CGWCG 1 cut(s) 124
HpyCH4III ACNGT 3 cut(s) 240, 335, 727
HpyCH4IV ACGT 3 cut(s) 754, 829, 1117
HpyCH4V TGCA 5 cut(s) 163, 184, 188, 931, 1084
HpyF10VI GCNNNNNNNGC 3 cut(s) 181, 598, 1069
HpyF3I CTNAG 6 cut(s) 144, 477, 513, 536, 588, 1073
HpySE526I ACGT 3 cut(s) 754, 829, 1117
Hsp92II CATG 5 cut(s) 188, 639, 646, 767, 958
KroI GCCGGC 1 cut(s) 1041
KroNI GCCGGC 1 cut(s) 1043
Kzo9I GATC 6 cut(s) 13, 193, 532, 583, 972, 1030
LmnI GCTCC 4 cut(s) 77, 886, 941, 1023
Lsp1109I GCAGC 4 cut(s) 97, 102, 604, 1124
LweI GCATC 2 cut(s) 130, 424
MaeI CTAG 4 cut(s) 558, 707, 1038, 1055
MaeII ACGT 3 cut(s) 754, 829, 1117
MaeIII GTNAC 1 cut(s) 813
MalI GATC 6 cut(s) 15, 195, 534, 585, 974, 1032
MbiI CCGCTC 1 cut(s) 99
MboI GATC 6 cut(s) 13, 193, 532, 583, 972, 1030
MboII GAAGA 6 cut(s) 243, 269, 644, 687, 913, 1091
MflI RGATCY 2 cut(s) 532, 972
MhlI GDGCHC 1 cut(s) 1139
MluCI AATT 6 cut(s) 447, 572, 646, 821, 1008, 1148
MmeI TCCRAC 2 cut(s) 335, 928
MroNI GCCGGC 1 cut(s) 1041
MroXI GAANNNNTTC 2 cut(s) 274, 771
MseI TTAA 5 cut(s) 129, 282, 446, 689, 759
MspA1I CMGCKG 1 cut(s) 512
MspI CCGG 3 cut(s) 75, 301, 1042
MspR9I CCNGG 2 cut(s) 301, 302
MwoI GCNNNNNNNGC 3 cut(s) 181, 598, 1069
NaeI GCCGGC 1 cut(s) 1043
NciI CCSGG 2 cut(s) 301, 302
NdeII GATC 6 cut(s) 13, 193, 532, 583, 972, 1030
NgoMIV GCCGGC 1 cut(s) 1041
NheI GCTAGC 1 cut(s) 1037
NlaIII CATG 5 cut(s) 188, 639, 646, 767, 958
NlaIV GGNNCC 2 cut(s) 501, 990
NmuCI GTSAC 1 cut(s) 813
NspI RCATGY 1 cut(s) 188
PaeI GCATGC 1 cut(s) 188
PagI TCATGA 1 cut(s) 954
PaqCI CACCTGC 1 cut(s) 922
PcsI WCGNNNNNNNCGW 1 cut(s) 202
PdiI GCCGGC 1 cut(s) 1043
PdmI GAANNNNTTC 2 cut(s) 274, 771
PfeI GAWTC 5 cut(s) 63, 296, 473, 904, 951
PflMI CCANNNNNTGG 1 cut(s) 967
PkrI GCNGC 5 cut(s) 87, 101, 117, 594, 1114
Ppu21I YACGTR 1 cut(s) 830
PsiI TTATAA 2 cut(s) 566, 779
PspFI CCCAGC 1 cut(s) 42
PspN4I GGNNCC 2 cut(s) 501, 990
PspPI GGNCC 2 cut(s) 262, 988
PstNI CAGNNNCTG 1 cut(s) 598
PsuI RGATCY 2 cut(s) 532, 972
PvuII CAGCTG 1 cut(s) 512
RsaI GTAC 1 cut(s) 79
RsaNI GTAC 1 cut(s) 78
SalI GTCGAC 1 cut(s) 121
SaqAI TTAA 5 cut(s) 129, 282, 446, 689, 759
SatI GCNGC 5 cut(s) 86, 100, 116, 593, 1113
Sau3AI GATC 6 cut(s) 13, 193, 532, 583, 972, 1030
Sau96I GGNCC 2 cut(s) 262, 988
ScrFI CCNGG 2 cut(s) 301, 302
SduI GDGCHC 1 cut(s) 1139
SfaNI GCATC 2 cut(s) 130, 424
SinI GGWCC 1 cut(s) 988
SmaI CCCGGG 1 cut(s) 302
SmlI CTYRAG 2 cut(s) 26, 133
SmoI CTYRAG 2 cut(s) 26, 133
SphI GCATGC 1 cut(s) 188
Sse9I AATT 6 cut(s) 447, 572, 646, 821, 1008, 1148
SsiI CCGC 3 cut(s) 58, 83, 99
SspMI CTAG 4 cut(s) 558, 707, 1038, 1055
StyD4I CCNGG 2 cut(s) 299, 300
StyI CCWWGG 2 cut(s) 265, 908
TaaI ACNGT 3 cut(s) 240, 335, 727
TaiI ACGT 3 cut(s) 757, 832, 1120
TaqI TCGA 5 cut(s) 6, 122, 167, 682, 1065
TasI AATT 6 cut(s) 447, 572, 646, 821, 1008, 1148
TauI GCSGC 1 cut(s) 102
TfiI GAWTC 5 cut(s) 63, 296, 473, 904, 951
Tru1I TTAA 5 cut(s) 129, 282, 446, 689, 759
Tru9I TTAA 5 cut(s) 129, 282, 446, 689, 759
TscAI CASTG 2 cut(s) 109, 1033
TseFI GTSAC 1 cut(s) 813
TseI GCWGC 4 cut(s) 85, 115, 592, 1112
Tsp45I GTSAC 1 cut(s) 813
TspDTI ATGAA 6 cut(s) 504, 659, 668, 780, 943, 971
TspMI CCCGGG 1 cut(s) 300
TspRI CASTG 2 cut(s) 109, 1033
Van91I CCANNNNNTGG 1 cut(s) 967
VpaK11BI GGWCC 1 cut(s) 988
XagI CCTNNNNNAGG 1 cut(s) 269
XapI RAATTY 2 cut(s) 572, 821
XbaI TCTAGA 1 cut(s) 1054
XceI RCATGY 1 cut(s) 188
XmaI CCCGGG 1 cut(s) 300
XmiI GTMKAC 1 cut(s) 122
XmnI GAANNNNTTC 2 cut(s) 274, 771
XspI CTAG 4 cut(s) 558, 707, 1038, 1055
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.