RchiOBHm_Chr4g0425351

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000660
Physical Location & Seq
Forward (+)
50960817 .. 50967376
6560 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39451

Sequence Viewer

Length: 2313 bp
ATGGACACTCCGCCGCCGCCGCCGGAGGAGCTGCTGAAGAAGATCCAGGTGCTGGAGGAGGGTCAGGAGCACCTGAAGCAGGAGATGTCCAAGCTCCGGAACAACTCCGATGCTAAGTCCGAGCACCCGAGGGCCCACTCGGTCTCGCCGCAGCGGTCCAGATTCTCGTCTTTGCCCAAGAGGAGAGTCGCCGGGGGCGGCGCCGTAGCTGGAGCCGACGCGGTGGGTTGGAAGAGAGGGTCTACTTCGTTTCGGCATTCTTCGCCGCTGCAGAGGGAGAGCCGCAGCCATGATCCTCCGACTGGCGGCAGCGGCCGCGCCGGCGATGATTTTAGAGGTGGAGGTGGTAGCGGGCCGTCGGCGGTGAACTTTACTGATAGGCAGTATCTGAATATATTGCAGTCCATTGGGCAGGCAGTGCATATTTTTGATCCCCAGGGTCGTATAATTTACTGGAACAAAACAGCTGAAGCTATGTATGGTTATTCAGCGGCAGAGGCCCTGGGACGGGATCCCATTGAGTTCTTAACAGATCCCCAGGACCATGTTGTAGGACATAATATAATCCAAAGAGTCACCAAGGGGGAGAGCTGGACAGGGCACTTCCCTGTCAAGAACAAAAAGGGGGAGAGATTTTCAGCTACTATAACGAATACTCCATTCTATGATGATGATGGCACTTTTATTGGGGTTATTTGTGTGTCAAGTGATACCAAGCCATTTCAAGAAATGAGGACGCACCCTGAACCAGATTCAAGCTTCAGTCGTACTAAAGCTACTGTTACAACTAAACTTGGTCTTGATCCTCAGCAGCCTCTGCAAACTGCAATTGCATCAAAAATTACAAATTTGGCATCCAAGATGAGCAACAAAGTCAAGTCGAAAATTCGGGCAGGAGAAAACTATATGGATCGGGAAGGTGTGAGTGTAGATAGTCATCATTCTGATCATGGTTCGGACTATGACCATCGGGAGGATGCAAATTCAAGTGGGGCTAGCACACCTAGAGGAGATATAGCCCCATCTCCTTTTGGTGTGTTTTCCCAGATGGATGAAAAGTCTCCAGGAAAACCTTCCAGAGATTCTGGCGATGAGAGTGAAGGAAAACCGCCAATGCACAAGGTAATATCCTCAAAGGCAGAAGCATGGATTGGTAAGAAAGGGATAACTTGGCCATGGAAAGGAAATGAGCGAGAAGGATCGGAGGTTAAGACCCCTCGTTTTCTTTGGCCTTGGTTGCAAAATGATCAAGAGAATGATCCAGTTCCTCAGAAGAATAATTGTGGTTTAAAACCTGACAATTACGCGACTGAAAGTAATCGACATGCAACTAATGAGGCTTCAGGATCCTGGACCTCCTCATTTAATGTTAACAGCACAAGCAGTGCCAGCAGCTGTGGAAGCACCAGCAGTACTGTGAATAAGGTAGACGTGGACACTGATTGCTTGGATTACGAAATCTTGTGGGAAGACTTGATGATTGGAGAACAGATTGGGCAAGGTTCATGTGGAACTGTATACCATGGTCTGTGGTACGGATCAGATGTTGCTGTCAAGGTATTCTCCAGGCAAGAATATTCAGAAGATGTGATACTTTCCTTCAGACAAGAGGTATCTCTTATGAAAAGGCTTCGACATCCAAATGTTCTGCTTTTCATGGGAGCTGTGACTTCTCCCGAGCGTCTTTGCATCGTAACAGAGTTCCTCCCACGTGGAAGTTTGTTTCGGTTACTACAAAGAAGCACAGCAAAACTAGATTGGAGACGACGTGTTCATATGGCTCTGGATATAGCACGAGGCATGAATTATCTTCATCATTTCAACCCTCCAATCATCCATCGAGATCTGAAGTCTTCAAATCTCCTAGTTGATAAGAACTGGACTGTCAAGGTTGGTGATTTTGGTCTTTCACGTCTAAAGCATGAAACATTTCTCACAACCAAGACTGGGAGGGGAACGCCCCAATGGATGGCACCAGAAGTTCTACGCAATGAACCCTCAGATGAGAAGTCGGATGTATATAGCTACGGAGTGATATTGTGGGAGCTTGTTACTGAAAAGATCCCTTGGGATAATCTAAACTCAATGCAGGTGATTGGAGCTGTAGGGTTCATGAACCAAAGACTAGAGATCCCAAAAGATGTTGACCCGCAGTGGATTTCTTTAATTGAGAGCTGCTGGCAAAGTGATCCCGCTAGCCGGCCAACATTTCAAGAGCTGCTGGAAAAGCTTCGAGATCTGCAGAGACAATACGCTCTTCAATTCCAAGCAGCGCGGTCCGCCGTAAGTGATAGCACCCAAAAAGAACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS PAS_9 Pkinase_Tyr

Protein Analysis

770

Amino Acids

85.95

Weight (kDa)

6.66

Isoelectric Point (pI)

50.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS PF00989 128 - 237 1.2e-14 PAS fold
PAS_8 PF13188 128 - 181 2.2e-07 PAS domain
PAS_4 PF08448 134 - 238 2e-13 PAS fold
PAS_9 PF13426 140 - 237 5.9e-13 PAS domain
PAS_3 PF08447 149 - 234 1.7e-06 PAS fold
PK_Tyr_Ser-Thr PF07714 493 - 744 2.4e-68 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 495 - 742 7.6e-58 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2080
Acc36I ACCTGC 1 cut(s) 2080
AccB1I GGYRCC 2 cut(s) 200, 1972
AccB7I CCANNNNNTGG 2 cut(s) 52, 1969
AccI GTMKAC 3 cut(s) 242, 1428, 1518
AccII CGCG 4 cut(s) 221, 318, 1307, 2275
AccIII TCCGGA 1 cut(s) 96
AcoI YGGCCR 3 cut(s) 313, 1172, 2201
AcsI RAATTY 3 cut(s) 847, 885, 982
AcuI CTGAAG 7 cut(s) 56, 95, 489, 745, 1326, 1585, 1868
AcvI CACGTG 1 cut(s) 1712
AcyI GRCGYC 1 cut(s) 201
AfaI GTAC 3 cut(s) 769, 1414, 1535
AflIII ACRYGT 1 cut(s) 1768
AgsI TTSAA 7 cut(s) 725, 756, 987, 1822, 1857, 2213, 2261
AjiI CACGTC 3 cut(s) 1432, 1769, 1913
AjnI CCWGG 7 cut(s) 45, 435, 501, 537, 1063, 1349, 1565
AjuI GAANNNNNNNTTGG 2 cut(s) 1134, 1166
Alw21I GWGCWC 2 cut(s) 72, 126
Alw26I GTCTC 4 cut(s) 148, 1065, 1756, 2239
AlwNI CAGNNNCTG 4 cut(s) 52, 388, 817, 1395
Ama87I CYCGRG 2 cut(s) 127, 1676
Aor13HI TCCGGA 1 cut(s) 96
AoxI GGCC 7 cut(s) 132, 313, 353, 498, 1172, 1229, 2201
ApaI GGGCCC 1 cut(s) 136
ApoI RAATTY 3 cut(s) 847, 885, 982
Asp700I GAANNNNTTC 3 cut(s) 1072, 1929, 2229
AspLEI GCGC 3 cut(s) 203, 320, 2275
AspS9I GGNCC 8 cut(s) 132, 133, 156, 353, 499, 541, 1353, 2277
AsuC2I CCSGG 1 cut(s) 193
AsuHPI GGTGA 4 cut(s) 376, 568, 1907, 2104
AsuNHI GCTAGC 2 cut(s) 995, 2195
AvaI CYCGRG 2 cut(s) 127, 1676
AvaII GGWCC 4 cut(s) 156, 541, 1353, 2277
BaeGI GKGCMC 2 cut(s) 136, 603
BalI TGGCCA 1 cut(s) 1174
BamHI GGATCC 2 cut(s) 511, 1346
BanI GGYRCC 2 cut(s) 200, 1972
BanII GRGCYC 1 cut(s) 136
BarI GAAGNNNNNNTAC 2 cut(s) 1575, 1607
BauI CACGAG 1 cut(s) 1794
BbrPI CACGTG 1 cut(s) 1712
BbsI GAAGAC 2 cut(s) 1476, 1845
Bbv12I GWGCWC 2 cut(s) 72, 126
BbvCI CCTCAGC 1 cut(s) 807
BccI CCATC 6 cut(s) 668, 975, 1030, 1042, 1845, 1963
BceAI ACGGC 3 cut(s) 188, 340, 2267
BciT130I CCWGG 7 cut(s) 47, 437, 503, 539, 1065, 1351, 1567
BclI TGATCA 2 cut(s) 946, 1246
BcnI CCSGG 1 cut(s) 193
BcoDI GTCTC 4 cut(s) 148, 1065, 1756, 2239
BfaI CTAG 6 cut(s) 996, 1005, 1754, 1865, 2126, 2196
BfmI CTRYAG 4 cut(s) 269, 2103, 2240, 2309
BfoI RGCGCY 1 cut(s) 204
BfuAI ACCTGC 1 cut(s) 2080
BglI GCCNNNNNGGC 1 cut(s) 321
BglII AGATCT 2 cut(s) 1843, 2236
BmcAI AGTACT 1 cut(s) 1414
Bme1390I CCNGG 8 cut(s) 47, 193, 437, 503, 539, 1065, 1351, 1567
Bme18I GGWCC 4 cut(s) 156, 541, 1353, 2277
BmeT110I CYCGRG 2 cut(s) 127, 1676
BmgBI CACGTC 3 cut(s) 1432, 1769, 1913
BmgT120I GGNCC 8 cut(s) 132, 133, 156, 353, 499, 541, 1353, 2277
BmiI GGNNCC 6 cut(s) 134, 202, 214, 513, 1348, 1974
BmrFI CCNGG 8 cut(s) 47, 193, 437, 503, 539, 1065, 1351, 1567
BmrI ACTGGG 1 cut(s) 1956
BmsI GCATC 5 cut(s) 100, 842, 863, 967, 1698
BmtI GCTAGC 2 cut(s) 999, 2199
BmuI ACTGGG 1 cut(s) 1956
BpiI GAAGAC 2 cut(s) 1476, 1845
BpmI CTGGAG 4 cut(s) 74, 231, 1047, 1549
Bpu10I CCTNAGC 1 cut(s) 807
BpuMI CCSGG 1 cut(s) 193
BsaAI YACGTR 1 cut(s) 1712
BsaBI GATNNNNATC 1 cut(s) 936
BsaHI GRCGYC 1 cut(s) 201
BsaI GGTCTC 1 cut(s) 148
BsaWI WCCGGW 1 cut(s) 96
BsaXI ACNNNNNCTCC 4 cut(s) 640, 670, 1753, 1783
Bse118I RCCGGY 2 cut(s) 320, 2199
Bse1I ACTGG 5 cut(s) 307, 458, 1262, 1883, 1951
Bse3DI GCAATG 1 cut(s) 1996
Bse8I GATNNNNATC 1 cut(s) 936
BseAI TCCGGA 1 cut(s) 96
BseBI CCWGG 7 cut(s) 47, 437, 503, 539, 1065, 1351, 1567
BseGI GGATG 7 cut(s) 854, 982, 1057, 1636, 1833, 1974, 2020
BseJI GATNNNNATC 1 cut(s) 936
BseMI GCAATG 1 cut(s) 1996
BseMII CTCAG 3 cut(s) 821, 1283, 2013
BseNI ACTGG 5 cut(s) 307, 458, 1262, 1883, 1951
BseRI GAGGAG 5 cut(s) 41, 71, 196, 1023, 1348
BseSI GKGCMC 2 cut(s) 136, 603
BseX3I CGGCCG 1 cut(s) 313
Bsh1236I CGCG 4 cut(s) 221, 318, 1307, 2275
Bsh1285I CGRYCG 1 cut(s) 316
BshFI GGCC 7 cut(s) 134, 315, 355, 500, 1174, 1231, 2203
BshNI GGYRCC 2 cut(s) 200, 1972
BsiEI CGRYCG 1 cut(s) 316
BsiHKAI GWGCWC 2 cut(s) 72, 126
BsiHKCI CYCGRG 2 cut(s) 127, 1676
BsiSI CCGG 5 cut(s) 23, 97, 192, 321, 2200
BslFI GGGAC 1 cut(s) 519
BsmAI GTCTC 4 cut(s) 148, 1065, 1756, 2239
BsmBI CGTCTC 1 cut(s) 1756
BsmFI GGGAC 1 cut(s) 519
BsmI GAATGC 1 cut(s) 256
BsnI GGCC 7 cut(s) 134, 315, 355, 500, 1174, 1231, 2203
Bso31I GGTCTC 1 cut(s) 148
BsoBI CYCGRG 2 cut(s) 127, 1676
Bsp120I GGGCCC 1 cut(s) 132
Bsp1286I GDGCHC 4 cut(s) 72, 126, 136, 603
Bsp13I TCCGGA 1 cut(s) 96
Bsp19I CCATGG 2 cut(s) 1175, 1522
BspANI GGCC 7 cut(s) 134, 315, 355, 500, 1174, 1231, 2203
BspCNI CTCAG 3 cut(s) 820, 1282, 2012
BspEI TCCGGA 1 cut(s) 96
BspFNI CGCG 4 cut(s) 221, 318, 1307, 2275
BspHI TCATGA 1 cut(s) 2112
BspLI GGNNCC 6 cut(s) 134, 202, 214, 513, 1348, 1974
BspMAI CTGCAG 2 cut(s) 273, 2244
BspMI ACCTGC 1 cut(s) 2080
BspOI GCTAGC 2 cut(s) 999, 2199
BspQI GCTCTTC 1 cut(s) 2262
BspT107I GGYRCC 2 cut(s) 200, 1972
BspTNI GGTCTC 1 cut(s) 148
BsrDI GCAATG 1 cut(s) 1996
BsrFI RCCGGY 2 cut(s) 320, 2199
BsrI ACTGG 5 cut(s) 307, 458, 1262, 1883, 1951
BssAI RCCGGY 2 cut(s) 320, 2199
BssNAI GTATAC 1 cut(s) 1519
BssNI GRCGYC 1 cut(s) 201
BssSI CACGAG 1 cut(s) 1794
BssT1I CCWWGG 5 cut(s) 579, 1175, 1232, 1522, 2066
Bst1107I GTATAC 1 cut(s) 1519
Bst2BI CACGAG 1 cut(s) 1794
Bst2UI CCWGG 7 cut(s) 47, 437, 503, 539, 1065, 1351, 1567
Bst4CI ACNGT 5 cut(s) 781, 1417, 1516, 1885, 2310
Bst6I CTCTTC 2 cut(s) 227, 2262
BstACI GRCGYC 1 cut(s) 201
BstAPI GCANNNNNTGC 2 cut(s) 418, 817
BstBAI YACGTR 1 cut(s) 1712
BstC8I GCNNGC 8 cut(s) 322, 353, 414, 997, 1390, 2180, 2197, 2201
BstDEI CTNAG 4 cut(s) 114, 807, 1269, 1999
BstDSI CCRYGG 2 cut(s) 1175, 1522
BstENI CCTNNNNNAGG 1 cut(s) 77
BstF5I GGATG 7 cut(s) 854, 982, 1057, 1636, 1833, 1974, 2020
BstFNI CGCG 4 cut(s) 221, 318, 1307, 2275
BstH2I RGCGCY 1 cut(s) 204
BstHHI GCGC 3 cut(s) 203, 320, 2275
BstMAI GTCTC 4 cut(s) 148, 1065, 1756, 2239
BstMCI CGRYCG 1 cut(s) 316
BstNI CCWGG 7 cut(s) 47, 437, 503, 539, 1065, 1351, 1567
BstNSI RCATGY 1 cut(s) 1328
BstSCI CCNGG 8 cut(s) 45, 191, 435, 501, 537, 1063, 1349, 1565
BstSFI CTRYAG 4 cut(s) 269, 2103, 2240, 2309
BstSLI GKGCMC 2 cut(s) 136, 603
BstUI CGCG 4 cut(s) 221, 318, 1307, 2275
BstV2I GAAGAC 2 cut(s) 1476, 1845
BstX2I RGATCY 8 cut(s) 42, 511, 532, 1346, 1843, 2061, 2130, 2236
BstYI RGATCY 8 cut(s) 42, 511, 532, 1346, 1843, 2061, 2130, 2236
BstZ17I GTATAC 1 cut(s) 1519
BstZI CGGCCG 1 cut(s) 313
BsuRI GGCC 7 cut(s) 134, 315, 355, 500, 1174, 1231, 2203
BtgI CCRYGG 2 cut(s) 1175, 1522
BtgZI GCGATG 2 cut(s) 339, 1104
BtrI CACGTC 3 cut(s) 1432, 1769, 1913
BtsCI GGATG 7 cut(s) 854, 982, 1057, 1636, 1833, 1974, 2020
BtsI GCAGTG 3 cut(s) 423, 1390, 2159
BtsIMutI CAGTG 4 cut(s) 423, 1390, 1437, 2159
BveI ACCTGC 1 cut(s) 2080
Cac8I GCNNGC 8 cut(s) 322, 353, 414, 997, 1390, 2180, 2197, 2201
CaiI CAGNNNCTG 4 cut(s) 52, 388, 817, 1395
CciI TCATGA 1 cut(s) 2112
CciNI GCGGCCGC 1 cut(s) 313
CfoI GCGC 3 cut(s) 203, 320, 2275
Cfr10I RCCGGY 2 cut(s) 320, 2199
Cfr13I GGNCC 8 cut(s) 132, 133, 156, 353, 499, 541, 1353, 2277
CpoI CGGWCCG 1 cut(s) 2277
CseI GACGC 3 cut(s) 227, 745, 1670
Csp6I GTAC 3 cut(s) 768, 1413, 1534
CspI CGGWCCG 1 cut(s) 2277
CviQI GTAC 3 cut(s) 768, 1413, 1534
DdeI CTNAG 4 cut(s) 114, 807, 1269, 1999
DinI GGCGCC 1 cut(s) 202
DraI TTTAAA 1 cut(s) 1290
EaeI YGGCCR 3 cut(s) 313, 1172, 2201
EagI CGGCCG 1 cut(s) 313
Eam1104I CTCTTC 2 cut(s) 227, 2262
EarI CTCTTC 2 cut(s) 227, 2262
EciI GGCGGA 1 cut(s) 2269
EclXI CGGCCG 1 cut(s) 313
Eco130I CCWWGG 5 cut(s) 579, 1175, 1232, 1522, 2066
Eco24I GRGCYC 1 cut(s) 136
Eco31I GGTCTC 1 cut(s) 148
Eco47I GGWCC 4 cut(s) 156, 541, 1353, 2277
Eco52I CGGCCG 1 cut(s) 313
Eco57I CTGAAG 7 cut(s) 56, 95, 489, 745, 1326, 1585, 1868
Eco72I CACGTG 1 cut(s) 1712
Eco88I CYCGRG 2 cut(s) 127, 1676
EcoNI CCTNNNNNAGG 1 cut(s) 77
EcoO109I RGGNCCY 2 cut(s) 132, 499
EcoRII CCWGG 7 cut(s) 45, 435, 501, 537, 1063, 1349, 1565
EcoT14I CCWWGG 5 cut(s) 579, 1175, 1232, 1522, 2066
EcoT38I GRGCYC 1 cut(s) 136
EgeI GGCGCC 1 cut(s) 202
EheI GGCGCC 1 cut(s) 202
ErhI CCWWGG 5 cut(s) 579, 1175, 1232, 1522, 2066
Esp3I CGTCTC 1 cut(s) 1756
FaqI GGGAC 1 cut(s) 519
FauI CCCGC 3 cut(s) 344, 2157, 2200
FauNDI CATATG 1 cut(s) 1776
FbaI TGATCA 2 cut(s) 946, 1246
FblI GTMKAC 3 cut(s) 242, 1428, 1518
FokI GGATG 7 cut(s) 841, 989, 1064, 1623, 1820, 1981, 2027
FriOI GRGCYC 1 cut(s) 136
FspBI CTAG 6 cut(s) 996, 1005, 1754, 1865, 2126, 2196
GlaI GCGC 3 cut(s) 202, 319, 2274
GsuI CTGGAG 4 cut(s) 74, 231, 1047, 1549
HaeII RGCGCY 1 cut(s) 204
HaeIII GGCC 7 cut(s) 134, 315, 355, 500, 1174, 1231, 2203
HapII CCGG 5 cut(s) 23, 97, 192, 321, 2200
HgaI GACGC 3 cut(s) 227, 745, 1670
HhaI GCGC 3 cut(s) 203, 320, 2275
Hin1I GRCGYC 1 cut(s) 201
Hin6I GCGC 3 cut(s) 201, 318, 2273
HinP1I GCGC 3 cut(s) 201, 318, 2273
HincII GTYRAC 2 cut(s) 1372, 2146
HindII GTYRAC 2 cut(s) 1372, 2146
HindIII AAGCTT 2 cut(s) 757, 2228
HinfI GANTC 5 cut(s) 162, 186, 573, 752, 1082
HpaI GTTAAC 1 cut(s) 1372
HpaII CCGG 5 cut(s) 23, 97, 192, 321, 2200
HphI GGTGA 4 cut(s) 376, 568, 1907, 2104
Hpy166II GTNNAC 7 cut(s) 243, 367, 1372, 1429, 1435, 1519, 2146
Hpy8I GTNNAC 7 cut(s) 243, 367, 1372, 1429, 1435, 1519, 2146
Hpy99I CGWCG 3 cut(s) 221, 361, 1770
HpyAV CCTTC 5 cut(s) 911, 1083, 1094, 1190, 1609
HpyCH4III ACNGT 5 cut(s) 781, 1417, 1516, 1885, 2310
HpyCH4IV ACGT 4 cut(s) 1431, 1711, 1768, 1912
HpyF3I CTNAG 4 cut(s) 114, 807, 1269, 1999
HpySE526I ACGT 4 cut(s) 1431, 1711, 1768, 1912
Hsp92I GRCGYC 1 cut(s) 201
HspAI GCGC 3 cut(s) 201, 318, 2273
KasI GGCGCC 1 cut(s) 200
Kpn2I TCCGGA 1 cut(s) 96
KroI GCCGGC 2 cut(s) 320, 2199
KroNI GCCGGC 2 cut(s) 322, 2201
Ksp22I TGATCA 2 cut(s) 946, 1246
KspAI GTTAAC 1 cut(s) 1372
LguI GCTCTTC 1 cut(s) 2262
LmnI GCTCC 7 cut(s) 28, 67, 99, 212, 1661, 2044, 2099
LweI GCATC 5 cut(s) 100, 842, 863, 967, 1698
MaeI CTAG 6 cut(s) 996, 1005, 1754, 1865, 2126, 2196
MaeII ACGT 4 cut(s) 1431, 1711, 1768, 1912
MaeIII GTNAC 6 cut(s) 574, 781, 1666, 1693, 1728, 2050
MfeI CAATTG 1 cut(s) 828
MflI RGATCY 8 cut(s) 42, 511, 532, 1346, 1843, 2061, 2130, 2236
MhlI GDGCHC 4 cut(s) 72, 126, 136, 603
MlsI TGGCCA 1 cut(s) 1174
MluNI TGGCCA 1 cut(s) 1174
Mly113I GGCGCC 1 cut(s) 201
MlyI GAGTC 2 cut(s) 195, 582
MmeI TCCRAC 3 cut(s) 209, 323, 1992
Mox20I TGGCCA 1 cut(s) 1174
MreI CGCCGGCG 1 cut(s) 320
MroI TCCGGA 1 cut(s) 96
MroNI GCCGGC 2 cut(s) 320, 2199
MroXI GAANNNNTTC 3 cut(s) 1072, 1929, 2229
MscI TGGCCA 1 cut(s) 1174
MseI TTAA 6 cut(s) 527, 1209, 1289, 1365, 1371, 2165
MslI CAYNNNNRTG 1 cut(s) 1641
Msp20I TGGCCA 1 cut(s) 1174
MspA1I CMGCKG 6 cut(s) 154, 268, 312, 467, 491, 1395
MspI CCGG 5 cut(s) 23, 97, 192, 321, 2200
MspR9I CCNGG 8 cut(s) 47, 193, 437, 503, 539, 1065, 1351, 1567
MunI CAATTG 1 cut(s) 828
Mva1269I GAATGC 1 cut(s) 256
MvaI CCWGG 7 cut(s) 47, 437, 503, 539, 1065, 1351, 1567
MvnI CGCG 4 cut(s) 221, 318, 1307, 2275
NaeI GCCGGC 2 cut(s) 322, 2201
NarI GGCGCC 1 cut(s) 201
NciI CCSGG 1 cut(s) 193
NcoI CCATGG 2 cut(s) 1175, 1522
NdeI CATATG 1 cut(s) 1776
NgoMIV GCCGGC 2 cut(s) 320, 2199
NheI GCTAGC 2 cut(s) 995, 2195
NlaIV GGNNCC 6 cut(s) 134, 202, 214, 513, 1348, 1974
NmuCI GTSAC 2 cut(s) 574, 1666
NotI GCGGCCGC 1 cut(s) 313
NspI RCATGY 1 cut(s) 1328
PagI TCATGA 1 cut(s) 2112
PaqCI CACCTGC 1 cut(s) 2080
PasI CCCWGGG 2 cut(s) 436, 502
PciSI GCTCTTC 1 cut(s) 2262
PctI GAATGC 1 cut(s) 256
PdiI GCCGGC 2 cut(s) 322, 2201
PdmI GAANNNNTTC 3 cut(s) 1072, 1929, 2229
PfeI GAWTC 3 cut(s) 162, 752, 1082
PflMI CCANNNNNTGG 2 cut(s) 52, 1969
PfoI TCCNGGA 2 cut(s) 1063, 1349
PleI GAGTC 2 cut(s) 194, 581
PluTI GGCGCC 1 cut(s) 204
PmaCI CACGTG 1 cut(s) 1712
PmlI CACGTG 1 cut(s) 1712
PpsI GAGTC 2 cut(s) 194, 581
Ppu21I YACGTR 1 cut(s) 1712
Psp6I CCWGG 7 cut(s) 45, 435, 501, 537, 1063, 1349, 1565
PspCI CACGTG 1 cut(s) 1712
PspGI CCWGG 7 cut(s) 45, 435, 501, 537, 1063, 1349, 1565
PspN4I GGNNCC 6 cut(s) 134, 202, 214, 513, 1348, 1974
PspOMI GGGCCC 1 cut(s) 132
PspPI GGNCC 8 cut(s) 132, 133, 156, 353, 499, 541, 1353, 2277
PstI CTGCAG 2 cut(s) 273, 2244
PstNI CAGNNNCTG 4 cut(s) 52, 388, 817, 1395
PsuI RGATCY 8 cut(s) 42, 511, 532, 1346, 1843, 2061, 2130, 2236
PvuII CAGCTG 2 cut(s) 467, 1395
RsaI GTAC 3 cut(s) 769, 1414, 1535
RsaNI GTAC 3 cut(s) 768, 1413, 1534
RseI CAYNNNNRTG 1 cut(s) 1641
Rsr2I CGGWCCG 1 cut(s) 2277
RsrII CGGWCCG 1 cut(s) 2277
SapI GCTCTTC 1 cut(s) 2262
SaqAI TTAA 6 cut(s) 527, 1209, 1289, 1365, 1371, 2165
Sau96I GGNCC 8 cut(s) 132, 133, 156, 353, 499, 541, 1353, 2277
ScaI AGTACT 1 cut(s) 1414
SchI GAGTC 2 cut(s) 195, 582
ScrFI CCNGG 8 cut(s) 47, 193, 437, 503, 539, 1065, 1351, 1567
SduI GDGCHC 4 cut(s) 72, 126, 136, 603
SfaNI GCATC 5 cut(s) 100, 842, 863, 967, 1698
SfcI CTRYAG 4 cut(s) 269, 2103, 2240, 2309
SfoI GGCGCC 1 cut(s) 202
SgrAI CRCCGGYG 1 cut(s) 320
SinI GGWCC 4 cut(s) 156, 541, 1353, 2277
SmiMI CAYNNNNRTG 1 cut(s) 1641
SspDI GGCGCC 1 cut(s) 200
SspI AATATT 1 cut(s) 1577
SspMI CTAG 6 cut(s) 996, 1005, 1754, 1865, 2126, 2196
StyD4I CCNGG 8 cut(s) 45, 191, 435, 501, 537, 1063, 1349, 1565
StyI CCWWGG 5 cut(s) 579, 1175, 1232, 1522, 2066
TaaI ACNGT 5 cut(s) 781, 1417, 1516, 1885, 2310
TaiI ACGT 4 cut(s) 1434, 1714, 1771, 1915
TaqI TCGA 5 cut(s) 881, 1321, 1633, 1840, 2233
TaqII GACCGA 1 cut(s) 130
TatI WGTACW 1 cut(s) 1412
TfiI GAWTC 3 cut(s) 162, 752, 1082
Tru1I TTAA 6 cut(s) 527, 1209, 1289, 1365, 1371, 2165
Tru9I TTAA 6 cut(s) 527, 1209, 1289, 1365, 1371, 2165
TscAI CASTG 4 cut(s) 423, 1390, 1444, 2159
TseFI GTSAC 2 cut(s) 574, 1666
Tsp45I GTSAC 2 cut(s) 574, 1666
TspGWI ACGGA 2 cut(s) 1551, 2043
TspRI CASTG 4 cut(s) 423, 1390, 1444, 2159
Van91I CCANNNNNTGG 2 cut(s) 52, 1969
VpaK11BI GGWCC 4 cut(s) 156, 541, 1353, 2277
XagI CCTNNNNNAGG 1 cut(s) 77
XapI RAATTY 3 cut(s) 847, 885, 982
XceI RCATGY 1 cut(s) 1328
XmiI GTMKAC 3 cut(s) 242, 1428, 1518
XmnI GAANNNNTTC 3 cut(s) 1072, 1929, 2229
XspI CTAG 6 cut(s) 996, 1005, 1754, 1865, 2126, 2196
ZrmI AGTACT 1 cut(s) 1414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.