MD16G1153700.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000660
Physical Location & Seq
Reverse (-)
12185028 .. 12190639
5612 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1153700.v1.1.491

Sequence Viewer

Length: 2337 bp
ATGGATACTCCGCCGCCGGAGGAGCTTCTGAAAAAGATCCAAGAGCTGGAAGCAGGCCAGGCCCACCTCAAGCAGGAGATCACCAAGCTCAGGCACTCTGGAGATGTCAAATCAAAGCACCAGAGGGCCCACTCGGTCTCGCCGCAGCGCTCGAGGTTCTCGTCCGTGCCCAGAAGGAGAGTCACTGGGGTGGGCGGTGCTGCTGGTAGTGGAGGTCACGATGCGGCGGCTTGGAAGAGAGGGTCTACTTCGTTTCGGCATTCCTCACCTCTTCAGAGGGAGAGCCACAGCCATGATCCTCCCACTGGCGGCAGTGGCGGTGGCGGCAGTGGAGGAAGCAGTAGCACGGGGCCTTCAGCTGTGAACTTTACTGATAGACAGTATCTGAACATACTACAGTCGATTGGGCAATCTGTGCATATATTTGATATCAATGGGATTATAATTTACTGGAACAGAACTGCTGAAAATTTATATGGGTATTCTGCGGCTGAGGCCCTTGGACATAGTCCTGTTGAGCTCTTATCAGATCCTCAAGACTACGGTGTCGCAAACAATATAATAAACCGTGTCACCAAGGGGGAGAGCTGGACCGGGCAGTTCCCTGTCAAGACCAAAACTGGGGAGAGATTTACGGCCTTTGTAACAAATACTCCATTCTATGATGATGATGGCACTTTTATTGGGATTATTTGCATGTCAAGTGATATCTGGCCTTTTCAAGAAATGAAGATTGCAATGTCAGGAGGAAAGCCCCCAGAATCAGATTCTAGCTACAGCCGGTCTAGAGCTTGTGTTACAGCTAAACTTGGCCTTGATCCTCAGCAGCCTCTACAAAATGCTATTGCATCAAAAATAACAAATTTGGCGTCCAAAATGAGCAACAAAGTAAAATCGAGAATTCGGGTGGGAGAAAACAACATAGATCACGAAGGTGAGGGCGATTGTCATCGTTCTGATCATGGTTCAGATTGTGTTCTCTCTGATCATAGGGAGGATGTGAATTCAAGTTGTGCTGCCACACCTAGAGGAGATTTGCCCCCATCTCCTTTCGGAGTATTCTCCCATATTGATGAAAAGTCTCCAGGAAAACCCTCCAGAGATTCTGGCGATGAGAGTAAAGGAAAACCTTCGATCCAGAGGATGATATCCTTGAAAGCAGAAGCATGGATGGGTAAGAAAGGGATAACATGGCCATGGAAAGGAAATGAGCCCGAAGAGCCAGAGACTAAGAGCACTCGTTTTGTTTCGCCTTGGTTGCCTAACGAACATGAGAATGATTCAGTTCATCAGAAGAATTATTTTGGTTCAAAACCAGAAAGTCAGGTGAATGAAAATAACCGGACTACAAATAATGAGGCCTCAGGATCCTGGACCTCCTCATTTAATGTTAACAGCACAAGCAGTGCCAGCAGCTGTGGAAGTACCAGCAGCAGTGCTGTGAATAAGGTGGATGTAGACACTGACAGTCTGGACTACGAAATCTTGTGGGAAGACTTGATAATTGGAGAACAAGTTGGGCAAGGATCATGTGGAACCGTATACCATGGTCTGTGGTATGGATCAGATGTTGCTGTGAAGGTATTCTCCTGGCAAGAATATTCAGAAGATGTGATATTTTCCTTCAGACAAGAGGTGTCTCTCATGAAAAGGCTTCGACATCCAAATGTTCTGCTTTTTATGGGAGCTGTGACTTCACCTCAGCGTCTCTGCATCGTAACAGAGTTCCTCCCTCGTGGAAGTTTGTTACACTTACTTCAAGGGAACACATCAAAACTATATTGGAGGCGACGTGTTCATATGGCGATAGATATAGCACGAGGCATGAATTATCTTCATCATTTCAACCCACCTATCATTCATCGAGATCTGAAGTCTTCAAATCTCCTTGTTGATAAAAACTGGACTGTTAAGGTTGGTGATTTTGGTCTTTCACGTCTTAAGCACGATACATTTCTCACAACCAAGACTGGGAAGGGCACGCCTCAATGGATGGCACCGGAAGTTCTACGTAATGAACCCTCAGACGAGAAGTCTGATATATACAGCTATGGGGTGATATTGTGGGAGCTTGCTACTGAGAAGATCCCTTGGGATAATCTGAACTCAATGCAGGTGATTGGAGCTGTAGGGTTCATGGACCAAAGGTTAGAGATCCCAAAGGACGTGGACCCGCAATGGACTTGTTTAATTGAGAGCTGCTGGCAGAGTGATGCAGCTTCCAGGCCAACATTTCAAGAACTGCTGGAGAAGCTCAGAGACCTGCAGAGACAATACACTCTTCAGTTCCAAGCAGCGCGTGCCGCCGCGGGTGATAACACCCCGAAAGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS PAS_9 Pkinase_Tyr

Protein Analysis

779

Amino Acids

86.23

Weight (kDa)

6.43

Isoelectric Point (pI)

49.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS PF00989 127 - 237 7.8e-15 PAS fold
PAS_4 PF08448 133 - 237 5.4e-12 PAS fold
PAS_9 PF13426 143 - 237 2.7e-11 PAS domain
PAS_3 PF08447 148 - 232 7.3e-07 PAS fold
PK_Tyr_Ser-Thr PF07714 502 - 752 3.8e-66 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 503 - 750 1.6e-53 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 443
AarI CACCTGC 1 cut(s) 2104
AasI GACNNNNNNGTC 1 cut(s) 545
Acc36I ACCTGC 2 cut(s) 2104, 2271
AccB1I GGYRCC 1 cut(s) 1996
AccB7I CCANNNNNTGG 1 cut(s) 46
AccI GTMKAC 3 cut(s) 245, 1458, 1542
AccII CGCG 2 cut(s) 2299, 2309
AcoI YGGCCR 1 cut(s) 1193
AcsI RAATTY 4 cut(s) 469, 862, 900, 1003
AcuI CTGAAG 5 cut(s) 257, 339, 1609, 1892, 2267
AcyI GRCGYC 1 cut(s) 869
AfaI GTAC 1 cut(s) 1426
AfeI AGCGCT 1 cut(s) 149
AfiI CCNNNNNNNGG 8 cut(s) 46, 73, 90, 305, 308, 621, 1202, 1971
AflII CTTAAG 1 cut(s) 1940
AflIII ACRYGT 1 cut(s) 1792
AgsI TTSAA 8 cut(s) 722, 1008, 1156, 1311, 1760, 1846, 1881, 2237
AhdI GACNNNNNGTC 1 cut(s) 2032
AjiI CACGTC 3 cut(s) 1793, 1937, 2167
AjnI CCWGG 5 cut(s) 57, 1084, 1370, 1589, 2222
AleI CACNNNNGTG 2 cut(s) 188, 933
Alw21I GWGCWC 2 cut(s) 522, 1238
Alw26I GTCTC 7 cut(s) 142, 1086, 1220, 1644, 1712, 2253, 2263
AlwNI CAGNNNCTG 2 cut(s) 385, 1416
Ama87I CYCGRG 1 cut(s) 151
Aor51HI AGCGCT 1 cut(s) 149
ApaI GGGCCC 1 cut(s) 130
ApeKI GCWGC 9 cut(s) 145, 200, 826, 1016, 1413, 1431, 2199, 2216, 2294
ApoI RAATTY 4 cut(s) 469, 862, 900, 1003
ArsI GACNNNNNNTTYG 2 cut(s) 854, 886
Asp700I GAANNNNTTC 2 cut(s) 1129, 1583
AspLEI GCGC 2 cut(s) 150, 2299
AspS9I GGNCC 9 cut(s) 61, 126, 127, 350, 496, 591, 1374, 2140, 2170
AsuC2I CCSGG 1 cut(s) 595
AvaI CYCGRG 1 cut(s) 151
AvaII GGWCC 4 cut(s) 591, 1374, 2140, 2170
AxyI CCTNAGG 1 cut(s) 1363
BaeGI GKGCMC 3 cut(s) 130, 171, 1982
BalI TGGCCA 1 cut(s) 1195
BamHI GGATCC 1 cut(s) 1367
BanI GGYRCC 1 cut(s) 1996
BanII GRGCYC 3 cut(s) 130, 522, 1215
BauI CACGAG 2 cut(s) 1734, 1818
BbsI GAAGAC 2 cut(s) 1500, 1869
Bbv12I GWGCWC 2 cut(s) 522, 1238
BbvCI CCTCAGC 3 cut(s) 492, 822, 1701
BbvI GCAGC 9 cut(s) 157, 187, 838, 1003, 1425, 1443, 2186, 2228, 2306
BccI CCATC 4 cut(s) 665, 1051, 1165, 1987
BceAI ACGGC 1 cut(s) 651
BciT130I CCWGG 5 cut(s) 59, 1086, 1372, 1591, 2224
BclI TGATCA 2 cut(s) 958, 985
BcnI CCSGG 1 cut(s) 595
BcoDI GTCTC 7 cut(s) 142, 1086, 1220, 1644, 1712, 2253, 2263
BfaI CTAG 3 cut(s) 771, 786, 1026
BfmI CTRYAG 4 cut(s) 395, 775, 2127, 2264
BfoI RGCGCY 1 cut(s) 151
BfrI CTTAAG 1 cut(s) 1940
BfuAI ACCTGC 2 cut(s) 2104, 2271
BglII AGATCT 1 cut(s) 1867
Bme1390I CCNGG 6 cut(s) 59, 595, 1086, 1372, 1591, 2224
Bme18I GGWCC 4 cut(s) 591, 1374, 2140, 2170
BmeRI GACNNNNNGTC 1 cut(s) 2032
BmeT110I CYCGRG 1 cut(s) 151
BmgBI CACGTC 3 cut(s) 1793, 1937, 2167
BmgT120I GGNCC 9 cut(s) 61, 126, 127, 350, 496, 591, 1374, 2140, 2170
BmiI GGNNCC 6 cut(s) 128, 351, 1369, 1537, 1998, 2172
BmrFI CCNGG 6 cut(s) 59, 595, 1086, 1372, 1591, 2224
BmrI ACTGGG 3 cut(s) 195, 630, 1980
BmsI GCATC 4 cut(s) 211, 857, 1722, 2203
BmuI ACTGGG 3 cut(s) 195, 630, 1980
BpiI GAAGAC 2 cut(s) 1500, 1869
BpmI CTGGAG 4 cut(s) 120, 1068, 1081, 2267
Bpu10I CCTNAGC 4 cut(s) 89, 492, 822, 1701
BpuEI CTTGAG 2 cut(s) 53, 519
BpuMI CCSGG 1 cut(s) 595
BsaAI YACGTR 1 cut(s) 2012
BsaBI GATNNNNATC 1 cut(s) 948
BsaHI GRCGYC 1 cut(s) 869
BsaI GGTCTC 2 cut(s) 142, 2253
BsaJI CCNNGG 7 cut(s) 499, 576, 1196, 1253, 1546, 2090, 2307
BsaWI WCCGGW 2 cut(s) 1341, 1999
BsaXI ACNNNNNCTCC 6 cut(s) 637, 667, 1500, 1530, 1777, 1807
Bsc4I CCNNNNNNNGG 8 cut(s) 46, 73, 90, 305, 308, 621, 1202, 1971
Bse118I RCCGGY 1 cut(s) 780
Bse1I ACTGG 6 cut(s) 190, 310, 455, 625, 1907, 1975
Bse21I CCTNAGG 1 cut(s) 1363
Bse3DI GCAATG 2 cut(s) 744, 2183
Bse8I GATNNNNATC 1 cut(s) 948
BseBI CCWGG 5 cut(s) 59, 1086, 1372, 1591, 2224
BseDI CCNNGG 7 cut(s) 499, 576, 1196, 1253, 1546, 2090, 2307
BseGI GGATG 6 cut(s) 1003, 1149, 1176, 1459, 1660, 1998
BseJI GATNNNNATC 1 cut(s) 948
BseLI CCNNNNNNNGG 8 cut(s) 46, 73, 90, 305, 308, 621, 1202, 1971
BseMI GCAATG 2 cut(s) 744, 2183
BseMII CTCAG 8 cut(s) 103, 483, 836, 1377, 1715, 2037, 2070, 2269
BseNI ACTGG 6 cut(s) 190, 310, 455, 625, 1907, 1975
BseRI GAGGAG 3 cut(s) 35, 1044, 1369
BseSI GKGCMC 3 cut(s) 130, 171, 1982
BseXI GCAGC 9 cut(s) 157, 187, 838, 1003, 1425, 1443, 2186, 2228, 2306
Bsh1236I CGCG 2 cut(s) 2299, 2309
BshNI GGYRCC 1 cut(s) 1996
BsiHKAI GWGCWC 2 cut(s) 522, 1238
BsiHKCI CYCGRG 1 cut(s) 151
BsiSI CCGG 5 cut(s) 17, 594, 781, 1342, 2000
BslI CCNNNNNNNGG 8 cut(s) 46, 73, 90, 305, 308, 621, 1202, 1971
BsmAI GTCTC 7 cut(s) 142, 1086, 1220, 1644, 1712, 2253, 2263
BsmBI CGTCTC 1 cut(s) 1712
BsmI GAATGC 1 cut(s) 259
Bso31I GGTCTC 2 cut(s) 142, 2253
BsoBI CYCGRG 1 cut(s) 151
Bsp120I GGGCCC 1 cut(s) 126
Bsp1286I GDGCHC 6 cut(s) 130, 171, 522, 1215, 1238, 1982
Bsp19I CCATGG 2 cut(s) 1196, 1546
BspCNI CTCAG 8 cut(s) 102, 484, 835, 1376, 1714, 2036, 2071, 2268
BspFNI CGCG 2 cut(s) 2299, 2309
BspHI TCATGA 1 cut(s) 1644
BspLI GGNNCC 6 cut(s) 128, 351, 1369, 1537, 1998, 2172
BspMAI CTGCAG 1 cut(s) 2268
BspMI ACCTGC 2 cut(s) 2104, 2271
BspQI GCTCTTC 1 cut(s) 1212
BspT107I GGYRCC 1 cut(s) 1996
BspTI CTTAAG 1 cut(s) 1940
BspTNI GGTCTC 2 cut(s) 142, 2253
BsrDI GCAATG 2 cut(s) 744, 2183
BsrFI RCCGGY 1 cut(s) 780
BsrI ACTGG 6 cut(s) 190, 310, 455, 625, 1907, 1975
BssAI RCCGGY 1 cut(s) 780
BssECI CCNNGG 7 cut(s) 499, 576, 1196, 1253, 1546, 2090, 2307
BssNAI GTATAC 1 cut(s) 1543
BssNI GRCGYC 1 cut(s) 869
BssSI CACGAG 2 cut(s) 1734, 1818
BssT1I CCWWGG 6 cut(s) 499, 576, 1196, 1253, 1546, 2090
Bst1107I GTATAC 1 cut(s) 1543
Bst2BI CACGAG 2 cut(s) 1734, 1818
Bst2UI CCWGG 5 cut(s) 59, 1086, 1372, 1591, 2224
Bst4CI ACNGT 7 cut(s) 381, 399, 545, 569, 1469, 1540, 1909
Bst6I CTCTTC 4 cut(s) 230, 276, 1212, 2286
BstACI GRCGYC 1 cut(s) 869
BstAFI CTTAAG 1 cut(s) 1940
BstAPI GCANNNNNTGC 2 cut(s) 415, 2300
BstBAI YACGTR 1 cut(s) 2012
BstC8I GCNNGC 6 cut(s) 55, 1411, 1982, 2073, 2204, 2301
BstDEI CTNAG 9 cut(s) 89, 492, 822, 1230, 1363, 1701, 2023, 2079, 2255
BstDSI CCRYGG 3 cut(s) 1196, 1546, 2307
BstENI CCTNNNNNAGG 1 cut(s) 71
BstF5I GGATG 6 cut(s) 1003, 1149, 1176, 1459, 1660, 1998
BstFNI CGCG 2 cut(s) 2299, 2309
BstH2I RGCGCY 1 cut(s) 151
BstHHI GCGC 2 cut(s) 150, 2299
BstMAI GTCTC 7 cut(s) 142, 1086, 1220, 1644, 1712, 2253, 2263
BstNI CCWGG 5 cut(s) 59, 1086, 1372, 1591, 2224
BstNSI RCATGY 1 cut(s) 700
BstSCI CCNGG 6 cut(s) 57, 593, 1084, 1370, 1589, 2222
BstSFI CTRYAG 4 cut(s) 395, 775, 2127, 2264
BstSLI GKGCMC 3 cut(s) 130, 171, 1982
BstSNI TACGTA 1 cut(s) 2012
BstUI CGCG 2 cut(s) 2299, 2309
BstV1I GCAGC 9 cut(s) 157, 187, 838, 1003, 1425, 1443, 2186, 2228, 2306
BstV2I GAAGAC 2 cut(s) 1500, 1869
BstX2I RGATCY 6 cut(s) 36, 529, 1367, 1867, 2085, 2154
BstYI RGATCY 6 cut(s) 36, 529, 1367, 1867, 2085, 2154
BstZ17I GTATAC 1 cut(s) 1543
Bsu36I CCTNAGG 1 cut(s) 1363
BtgI CCRYGG 3 cut(s) 1196, 1546, 2307
BtgZI GCGATG 1 cut(s) 1125
BtrI CACGTC 3 cut(s) 1793, 1937, 2167
BtsCI GGATG 6 cut(s) 1003, 1149, 1176, 1459, 1660, 1998
BtsI GCAGTG 4 cut(s) 319, 334, 1411, 1441
BtsIMutI CAGTG 7 cut(s) 183, 303, 319, 334, 1411, 1441, 1461
BveI ACCTGC 2 cut(s) 2104, 2271
Cac8I GCNNGC 6 cut(s) 55, 1411, 1982, 2073, 2204, 2301
CaiI CAGNNNCTG 2 cut(s) 385, 1416
CciI TCATGA 1 cut(s) 1644
CfoI GCGC 2 cut(s) 150, 2299
Cfr10I RCCGGY 1 cut(s) 780
Cfr13I GGNCC 9 cut(s) 61, 126, 127, 350, 496, 591, 1374, 2140, 2170
Cfr42I CCGCGG 1 cut(s) 2310
CseI GACGC 2 cut(s) 858, 1694
Csp6I GTAC 1 cut(s) 1425
CspCI CAANNNNNGTGG 2 cut(s) 2148, 2183
CviQI GTAC 1 cut(s) 1425
DdeI CTNAG 9 cut(s) 89, 492, 822, 1230, 1363, 1701, 2023, 2079, 2255
DrdI GACNNNNNNGTC 1 cut(s) 545
DriI GACNNNNNGTC 1 cut(s) 2032
DseDI GACNNNNNNGTC 1 cut(s) 545
EaeI YGGCCR 1 cut(s) 1193
Eam1104I CTCTTC 4 cut(s) 230, 276, 1212, 2286
Eam1105I GACNNNNNGTC 1 cut(s) 2032
EarI CTCTTC 4 cut(s) 230, 276, 1212, 2286
Ecl136II GAGCTC 1 cut(s) 520
Eco105I TACGTA 1 cut(s) 2012
Eco130I CCWWGG 6 cut(s) 499, 576, 1196, 1253, 1546, 2090
Eco147I AGGCCT 1 cut(s) 1361
Eco24I GRGCYC 3 cut(s) 130, 522, 1215
Eco31I GGTCTC 2 cut(s) 142, 2253
Eco32I GATATC 3 cut(s) 430, 709, 1149
Eco47I GGWCC 4 cut(s) 591, 1374, 2140, 2170
Eco47III AGCGCT 1 cut(s) 149
Eco53kI GAGCTC 1 cut(s) 520
Eco57I CTGAAG 5 cut(s) 257, 339, 1609, 1892, 2267
Eco81I CCTNAGG 1 cut(s) 1363
Eco88I CYCGRG 1 cut(s) 151
EcoICRI GAGCTC 1 cut(s) 520
EcoNI CCTNNNNNAGG 1 cut(s) 71
EcoO109I RGGNCCY 3 cut(s) 126, 350, 496
EcoRI GAATTC 2 cut(s) 900, 1003
EcoRII CCWGG 5 cut(s) 57, 1084, 1370, 1589, 2222
EcoRV GATATC 3 cut(s) 430, 709, 1149
EcoT14I CCWWGG 6 cut(s) 499, 576, 1196, 1253, 1546, 2090
EcoT38I GRGCYC 3 cut(s) 130, 522, 1215
ErhI CCWWGG 6 cut(s) 499, 576, 1196, 1253, 1546, 2090
Esp3I CGTCTC 1 cut(s) 1712
FauI CCCGC 2 cut(s) 2181, 2302
FauNDI CATATG 1 cut(s) 1800
FbaI TGATCA 2 cut(s) 958, 985
FblI GTMKAC 3 cut(s) 245, 1458, 1542
FokI GGATG 6 cut(s) 1010, 1156, 1183, 1466, 1647, 2005
FriOI GRGCYC 3 cut(s) 130, 522, 1215
FspBI CTAG 3 cut(s) 771, 786, 1026
GlaI GCGC 2 cut(s) 149, 2298
GsuI CTGGAG 4 cut(s) 120, 1068, 1081, 2267
HaeII RGCGCY 1 cut(s) 151
HapII CCGG 5 cut(s) 17, 594, 781, 1342, 2000
HgaI GACGC 2 cut(s) 858, 1694
HhaI GCGC 2 cut(s) 150, 2299
Hin1I GRCGYC 1 cut(s) 869
Hin6I GCGC 2 cut(s) 148, 2297
HinP1I GCGC 2 cut(s) 148, 2297
HincII GTYRAC 1 cut(s) 1393
HindII GTYRAC 1 cut(s) 1393
HinfI GANTC 5 cut(s) 180, 761, 767, 1103, 1280
HpaI GTTAAC 1 cut(s) 1393
HpaII CCGG 5 cut(s) 17, 594, 781, 1342, 2000
Hpy166II GTNNAC 6 cut(s) 246, 364, 1393, 1459, 1543, 2170
Hpy8I GTNNAC 6 cut(s) 246, 364, 1393, 1459, 1543, 2170
Hpy99I CGWCG 1 cut(s) 1794
HpyAV CCTTC 7 cut(s) 168, 363, 926, 1140, 1573, 1633, 1969
HpyCH4III ACNGT 7 cut(s) 381, 399, 545, 569, 1469, 1540, 1909
HpyCH4IV ACGT 4 cut(s) 1792, 1936, 2011, 2166
HpyCH4V TGCA 8 cut(s) 418, 696, 737, 848, 1713, 2113, 2216, 2266
HpyF3I CTNAG 9 cut(s) 89, 492, 822, 1230, 1363, 1701, 2023, 2079, 2255
HpySE526I ACGT 4 cut(s) 1792, 1936, 2011, 2166
Hsp92I GRCGYC 1 cut(s) 869
HspAI GCGC 2 cut(s) 148, 2297
Ksp22I TGATCA 2 cut(s) 958, 985
KspAI GTTAAC 1 cut(s) 1393
KspI CCGCGG 1 cut(s) 2310
LguI GCTCTTC 1 cut(s) 1212
LmnI GCTCC 4 cut(s) 22, 1685, 2068, 2123
Lsp1109I GCAGC 9 cut(s) 157, 187, 838, 1003, 1425, 1443, 2186, 2228, 2306
LweI GCATC 4 cut(s) 211, 857, 1722, 2203
MaeI CTAG 3 cut(s) 771, 786, 1026
MaeII ACGT 4 cut(s) 1792, 1936, 2011, 2166
MaeIII GTNAC 8 cut(s) 181, 215, 571, 643, 796, 1690, 1717, 1746
MflI RGATCY 6 cut(s) 36, 529, 1367, 1867, 2085, 2154
MhlI GDGCHC 6 cut(s) 130, 171, 522, 1215, 1238, 1982
MlsI TGGCCA 1 cut(s) 1195
MluNI TGGCCA 1 cut(s) 1195
MlyI GAGTC 1 cut(s) 189
Mox20I TGGCCA 1 cut(s) 1195
MroXI GAANNNNTTC 2 cut(s) 1129, 1583
MscI TGGCCA 1 cut(s) 1195
MseI TTAA 5 cut(s) 1386, 1392, 1911, 1941, 2189
MslI CAYNNNNRTG 7 cut(s) 188, 291, 933, 1071, 1195, 1275, 1665
Msp20I TGGCCA 1 cut(s) 1195
MspA1I CMGCKG 3 cut(s) 359, 1416, 2309
MspCI CTTAAG 1 cut(s) 1940
MspI CCGG 5 cut(s) 17, 594, 781, 1342, 2000
MspR9I CCNGG 6 cut(s) 59, 595, 1086, 1372, 1591, 2224
Mva1269I GAATGC 1 cut(s) 259
MvaI CCWGG 5 cut(s) 59, 1086, 1372, 1591, 2224
MvnI CGCG 2 cut(s) 2299, 2309
NciI CCSGG 1 cut(s) 595
NcoI CCATGG 2 cut(s) 1196, 1546
NdeI CATATG 1 cut(s) 1800
NlaIV GGNNCC 6 cut(s) 128, 351, 1369, 1537, 1998, 2172
NmuCI GTSAC 4 cut(s) 181, 215, 571, 1690
NspI RCATGY 1 cut(s) 700
OliI CACNNNNGTG 2 cut(s) 188, 933
PaeR7I CTCGAG 1 cut(s) 151
PagI TCATGA 1 cut(s) 1644
PaqCI CACCTGC 1 cut(s) 2104
PceI AGGCCT 1 cut(s) 1361
PciSI GCTCTTC 1 cut(s) 1212
PcsI WCGNNNNNNNCGW 1 cut(s) 158
PctI GAATGC 1 cut(s) 259
PdmI GAANNNNTTC 2 cut(s) 1129, 1583
PfeI GAWTC 4 cut(s) 761, 767, 1103, 1280
PflFI GACNNNGTC 1 cut(s) 507
PflMI CCANNNNNTGG 1 cut(s) 46
PfoI TCCNGGA 2 cut(s) 1084, 1370
PleI GAGTC 1 cut(s) 188
PpsI GAGTC 1 cut(s) 188
Ppu21I YACGTR 1 cut(s) 2012
PsiI TTATAA 1 cut(s) 443
Psp124BI GAGCTC 1 cut(s) 522
Psp6I CCWGG 5 cut(s) 57, 1084, 1370, 1589, 2222
PspGI CCWGG 5 cut(s) 57, 1084, 1370, 1589, 2222
PspN4I GGNNCC 6 cut(s) 128, 351, 1369, 1537, 1998, 2172
PspOMI GGGCCC 1 cut(s) 126
PspPI GGNCC 9 cut(s) 61, 126, 127, 350, 496, 591, 1374, 2140, 2170
PspXI VCTCGAGB 1 cut(s) 151
PstI CTGCAG 1 cut(s) 2268
PstNI CAGNNNCTG 2 cut(s) 385, 1416
PsuI RGATCY 6 cut(s) 36, 529, 1367, 1867, 2085, 2154
PsyI GACNNNGTC 1 cut(s) 507
PvuII CAGCTG 2 cut(s) 359, 1416
RsaI GTAC 1 cut(s) 1426
RsaNI GTAC 1 cut(s) 1425
RseI CAYNNNNRTG 7 cut(s) 188, 291, 933, 1071, 1195, 1275, 1665
SacI GAGCTC 1 cut(s) 522
SacII CCGCGG 1 cut(s) 2310
SapI GCTCTTC 1 cut(s) 1212
SaqAI TTAA 5 cut(s) 1386, 1392, 1911, 1941, 2189
Sau96I GGNCC 9 cut(s) 61, 126, 127, 350, 496, 591, 1374, 2140, 2170
SchI GAGTC 1 cut(s) 189
ScrFI CCNGG 6 cut(s) 59, 595, 1086, 1372, 1591, 2224
SduI GDGCHC 6 cut(s) 130, 171, 522, 1215, 1238, 1982
SfaNI GCATC 4 cut(s) 211, 857, 1722, 2203
SfcI CTRYAG 4 cut(s) 395, 775, 2127, 2264
Sfr274I CTCGAG 1 cut(s) 151
Sfr303I CCGCGG 1 cut(s) 2310
SgrBI CCGCGG 1 cut(s) 2310
SinI GGWCC 4 cut(s) 591, 1374, 2140, 2170
SlaI CTCGAG 1 cut(s) 151
SmiMI CAYNNNNRTG 7 cut(s) 188, 291, 933, 1071, 1195, 1275, 1665
SmlI CTYRAG 4 cut(s) 68, 151, 534, 1940
SmoI CTYRAG 4 cut(s) 68, 151, 534, 1940
SnaBI TACGTA 1 cut(s) 2012
SseBI AGGCCT 1 cut(s) 1361
SspI AATATT 1 cut(s) 1601
SspMI CTAG 3 cut(s) 771, 786, 1026
SstI GAGCTC 1 cut(s) 522
StuI AGGCCT 1 cut(s) 1361
StyD4I CCNGG 6 cut(s) 57, 593, 1084, 1370, 1589, 2222
StyI CCWWGG 6 cut(s) 499, 576, 1196, 1253, 1546, 2090
TaaI ACNGT 7 cut(s) 381, 399, 545, 569, 1469, 1540, 1909
TaiI ACGT 4 cut(s) 1795, 1939, 2014, 2169
TaqI TCGA 6 cut(s) 152, 401, 896, 1133, 1657, 1864
TaqII GACCGA 1 cut(s) 124
TauI GCSGC 9 cut(s) 16, 145, 227, 230, 312, 327, 491, 2306, 2309
TfiI GAWTC 4 cut(s) 761, 767, 1103, 1280
Tru1I TTAA 5 cut(s) 1386, 1392, 1911, 1941, 2189
Tru9I TTAA 5 cut(s) 1386, 1392, 1911, 1941, 2189
TscAI CASTG 7 cut(s) 190, 310, 319, 334, 1411, 1441, 1468
TseFI GTSAC 4 cut(s) 181, 215, 571, 1690
TseI GCWGC 9 cut(s) 145, 200, 826, 1016, 1413, 1431, 2199, 2216, 2294
Tsp45I GTSAC 4 cut(s) 181, 215, 571, 1690
TspGWI ACGGA 1 cut(s) 154
TspRI CASTG 7 cut(s) 190, 310, 319, 334, 1411, 1441, 1468
Tth111I GACNNNGTC 1 cut(s) 507
Van91I CCANNNNNTGG 1 cut(s) 46
Vha464I CTTAAG 1 cut(s) 1940
VpaK11BI GGWCC 4 cut(s) 591, 1374, 2140, 2170
XagI CCTNNNNNAGG 1 cut(s) 71
XapI RAATTY 4 cut(s) 469, 862, 900, 1003
XbaI TCTAGA 1 cut(s) 785
XceI RCATGY 1 cut(s) 700
XhoI CTCGAG 1 cut(s) 151
XmiI GTMKAC 3 cut(s) 245, 1458, 1542
XmnI GAANNNNTTC 2 cut(s) 1129, 1583
XspI CTAG 3 cut(s) 771, 786, 1026
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.