RchiOBHm_Chr4g0425311

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000660
Physical Location & Seq
Forward (+)
50915391 .. 50920496
5106 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39447

Sequence Viewer

Length: 2166 bp
ATGGTCGAAAGTGATCTCGCCCACCTCAAGCAAGAAATGTCCCAGCTCACCAGCCAACGCCGAATCGCCAGCTCCGGTACTCCGCAGCCCAAAACGAGCGGCAGTGCCAGTCGGCTGCCGTCGACTTTTAACTTGAGTGATGCTCAGTATCTCAACATATTGCAGTCGATTGGGCAGTCTGTGCATGCAATGGATCGTAGTGGTCGTATAATCTACTGTATGAAAACTCAAAATTGCAGGAACAGAAGTGCTGAAAAACTGTATGGTTATTCTTCGGAAGAGGCCCTTGGAAAGGTTTCCATTAAGCTCATTGTAGATTCCCGGGACTTTGGTGTAGCATATAATATAGTCCACCGTGTCATCCACGGGGAGAGTTGGACTGGGCAGTTCCCTGTAAAGCACAAATCGGGGGCGAGGTTTTCAGTCATTACAACAAATACTCCATTCTATGATGATGCTGGCAGTTTAATTGGGGTTGTTGCTACATCAACTGATTCTCAGCCCTTTTATGAAATGAGGGTTCCAATGTCAGCTGAGAAGCAACCACAACAAGATCTAAGCTTCAGCAGTCGTGAGGCTAGTGTTACAACTAAATTCGGTGCTGATCCTCAGCAGCCTCTGCCAAAAGCAGTTTTGTCAAAACTAACAGACTTGCTGTGTGTCGTGATTGTTATAATCGTGAAACAGGCATCGAAGATGAGTTTCAAAGTCAAGTCGAAAATTCAGGCAAGAGAAAACAACATGGATCCCAAAGGTGACAGCGAAGATAGTCATCATCCGTTTCACGGTTCAGATTCTGTCATATCTGCCAATAGGGAGGATGGGAATGCAAGTGGGACTAGCACAGCTATAGGAACTATACCCCCAGCAACTCCTTTTGGTGTATTTAACCATGTTGAGAGCAAGCCTCTAGGAGAACCCTCCATAGATTCTGCCAGTGAGAGCAAAGAAAAATCTTCAATTCACAAGCTAATTGCCTCAAAGGCAGAAGCATGGATTAGTAAGCAGGAAATATCATGGCCTTGGAAAGGAAATGAGCGAGATGGGTCAGTGGCTAGGGCCAGTGGTTTTGTTTGGCCCTGGTTTCATAATGATCAAGTCAATGTTGCCATTCCTCAGAAGAGTTATTGTGTCTCAGAACCAGAAATTCAGGTGAACGAAAGTAATGGGCCAACAAATGATAATGATGAGACTCAAGGGTCCTGGACCTCTCCATTGGATATTAACAGCATAAGCTGTGCCATCAGCCTTAGAAGCACCAGTAGTGCTTTGGCTACGGTGGATGTGGACACTGATTGCTTGGATTATGAAATCTTATGGGAAGACATGATAATTGGAAGAAAGATTGGGCAAGGTTCATGTGGAACTGTATACCATGGTCAGTGGGATGGATCAGATGTTGCCATTAAGGTATTCTCCAAGTTTGAATATTCGGATGATTTGTTACTTTCCTTCAAACAAGAGGTATCCCTAATGAAAAGGCTTCGACATCCAAATGTTTTGCTTTTCATGGGAGCGGTGACTTCACCTGAGCGTCTCTGCATTGTCACAGAGTTCCTCCCACGCGGAAGTTTGTATCAAATACTACAGAGGAACCCATCAAAACTAGACTGGAGACGACGTATTAATATGGCTATGGATATAGCATGGGGCATGAATTATCTTCACCATTTCAACCCACCAATCATTCATCGAGACTTAAAGTCTTCAAATCTCCTAGTTGATAAGAACTGGACTGTCAAGGTCGGCGATTTTGGTCTTTCACGTTTTAAGCATGAAACTTTTCTTATAAACAAGAGTGGCAGGGGAACGCCTCAATGGATGTCACCAGAATTTCTACGTAGTGAACCCTCAGATGAGAAGTCTGACATATATAGCTACGGGGTGATATTGTGGGAGCTTGCTACCGAGAAGATTCCTTGGGATAATCTAAACCCAATGCAGGTGATTGGAGCTGTTGGATTCATGAACCAAAGACTGGAGATCCCAAAAGATGTGGACCCAAAGTGGGCTTCTATAATTGAGAGTTGCTGGCGCAGTGATCCAGCTAGCCGGCCAACATTTCTAGAACTGCTCGAAAAGCTTAGAGTGTTGCATAGACAATATAATCTTCAGTTCAAAGCAGCACGTTCCGCCTCTAATGAGAGCACCCAAAAAGAATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS PAS_9 Pkinase_Tyr

Protein Analysis

721

Amino Acids

80.67

Weight (kDa)

6.67

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 445 - 695 8.8e-66 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 446 - 693 3.8e-52 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 674, 1790
AarI CACCTGC 1 cut(s) 1933
AasI GACNNNNNNGTC 1 cut(s) 1198
Acc36I ACCTGC 1 cut(s) 1933
AccB7I CCANNNNNTGG 1 cut(s) 1978
AccBSI CCGCTC 2 cut(s) 99, 1517
AccI GTMKAC 2 cut(s) 122, 1371
AccII CGCG 1 cut(s) 1566
AciI CCGC 5 cut(s) 83, 99, 1517, 1566, 2133
AclWI GGATC 7 cut(s) 201, 599, 740, 753, 1399, 1978, 2036
AcoI YGGCCR 1 cut(s) 2054
AcsI RAATTY 4 cut(s) 593, 720, 1146, 1832
AcuI CTGAAG 2 cut(s) 547, 2096
AfaI GTAC 1 cut(s) 79
AfiI CCNNNNNNNGG 6 cut(s) 292, 785, 1028, 1942, 1978, 2008
AgsI TTSAA 7 cut(s) 706, 960, 1427, 1456, 1675, 1710, 2119
AhdI GACNNNNNGTC 1 cut(s) 1702
AjnI CCWGG 2 cut(s) 1079, 1202
AjuI GAANNNNNNNTTGG 4 cut(s) 270, 302, 1996, 2028
Alw21I GWGCWC 1 cut(s) 2150
Alw26I GTCTC 5 cut(s) 1138, 1184, 1541, 1609, 1689
AlwI GGATC 7 cut(s) 201, 599, 740, 753, 1399, 1978, 2036
AlwNI CAGNNNCTG 2 cut(s) 619, 797
Ama87I CYCGRG 1 cut(s) 321
AoxI GGCC 6 cut(s) 282, 1019, 1059, 1076, 1169, 2054
ApeKI GCWGC 4 cut(s) 85, 115, 613, 2123
ApoI RAATTY 4 cut(s) 593, 720, 1146, 1832
AseI ATTAAT 1 cut(s) 1626
Asp700I GAANNNNTTC 2 cut(s) 295, 1782
AspLEI GCGC 1 cut(s) 2037
AspS9I GGNCC 7 cut(s) 283, 1059, 1077, 1169, 1200, 1206, 1999
AsuC2I CCSGG 2 cut(s) 322, 323
AsuHPI GGTGA 9 cut(s) 40, 767, 1165, 1518, 1531, 1658, 1818, 1897, 1957
AsuNHI GCTAGC 1 cut(s) 2048
AvaI CYCGRG 1 cut(s) 321
AvaII GGWCC 3 cut(s) 1200, 1206, 1999
BamHI GGATCC 1 cut(s) 745
BbsI GAAGAC 2 cut(s) 1329, 1698
Bbv12I GWGCWC 1 cut(s) 2150
BbvCI CCTCAGC 1 cut(s) 609
BbvI GCAGC 4 cut(s) 97, 102, 625, 2135
BccI CCATC 5 cut(s) 815, 1037, 1250, 1382, 1606
BceAI ACGGC 1 cut(s) 103
BciT130I CCWGG 2 cut(s) 1081, 1204
BciVI GTATCC 1 cut(s) 1477
BclI TGATCA 1 cut(s) 1093
BcnI CCSGG 2 cut(s) 322, 323
BcoDI GTCTC 5 cut(s) 1138, 1184, 1541, 1609, 1689
BfaI CTAG 8 cut(s) 579, 840, 911, 1056, 1607, 1718, 2049, 2066
BfmI CTRYAG 2 cut(s) 849, 1586
BfuAI ACCTGC 1 cut(s) 1933
BfuI GTATCC 1 cut(s) 1477
BglI GCCNNNNNGGC 1 cut(s) 983
BglII AGATCT 1 cut(s) 553
BisI GCNGC 5 cut(s) 86, 100, 116, 614, 2124
BlsI GCNGC 5 cut(s) 87, 101, 117, 615, 2125
Bme1390I CCNGG 4 cut(s) 322, 323, 1081, 1204
Bme18I GGWCC 3 cut(s) 1200, 1206, 1999
BmeRI GACNNNNNGTC 1 cut(s) 1702
BmeT110I CYCGRG 1 cut(s) 321
BmgT120I GGNCC 7 cut(s) 283, 1059, 1077, 1169, 1200, 1206, 1999
BmiI GGNNCC 5 cut(s) 522, 747, 1201, 1595, 2001
BmrFI CCNGG 4 cut(s) 322, 323, 1081, 1204
BmrI ACTGGG 1 cut(s) 390
BmsI GCATC 3 cut(s) 130, 445, 698
BmtI GCTAGC 1 cut(s) 2052
BmuI ACTGGG 1 cut(s) 390
BpiI GAAGAC 2 cut(s) 1329, 1698
BplI GAGNNNNNCTC 4 cut(s) 127, 159, 892, 924
BpmI CTGGAG 2 cut(s) 1633, 2000
Bpu10I CCTNAGC 2 cut(s) 609, 1530
BpuEI CTTGAG 3 cut(s) 11, 154, 1179
BpuMI CCSGG 2 cut(s) 322, 323
BsaAI YACGTR 1 cut(s) 1841
BsaBI GATNNNNATC 1 cut(s) 771
BsaJI CCNNGG 7 cut(s) 286, 321, 364, 1022, 1079, 1375, 1919
BsaWI WCCGGW 1 cut(s) 74
BsaXI ACNNNNNCTCC 4 cut(s) 424, 454, 1606, 1636
Bsc4I CCNNNNNNNGG 6 cut(s) 292, 785, 1028, 1942, 1978, 2008
Bse118I RCCGGY 1 cut(s) 2052
Bse1I ACTGG 8 cut(s) 108, 385, 936, 1062, 1260, 1616, 1736, 1983
Bse3DI GCAATG 1 cut(s) 195
Bse8I GATNNNNATC 1 cut(s) 771
BseBI CCWGG 2 cut(s) 1081, 1204
BseDI CCNNGG 7 cut(s) 286, 321, 364, 1022, 1079, 1375, 1919
BseGI GGATG 8 cut(s) 360, 775, 826, 1288, 1393, 1441, 1489, 1827
BseJI GATNNNNATC 1 cut(s) 771
BseLI CCNNNNNNNGG 6 cut(s) 292, 785, 1028, 1942, 1978, 2008
BseMI GCAATG 1 cut(s) 195
BseMII CTCAG 8 cut(s) 158, 512, 525, 623, 1130, 1149, 1521, 1866
BseNI ACTGG 8 cut(s) 108, 385, 936, 1062, 1260, 1616, 1736, 1983
BseXI GCAGC 4 cut(s) 97, 102, 625, 2135
BseYI CCCAGC 2 cut(s) 42, 865
Bsh1236I CGCG 1 cut(s) 1566
BshFI GGCC 6 cut(s) 284, 1021, 1061, 1078, 1171, 2056
BsiHKAI GWGCWC 1 cut(s) 2150
BsiHKCI CYCGRG 1 cut(s) 321
BsiSI CCGG 3 cut(s) 75, 322, 2053
BslFI GGGAC 3 cut(s) 25, 338, 850
BslI CCNNNNNNNGG 6 cut(s) 292, 785, 1028, 1942, 1978, 2008
BsmAI GTCTC 5 cut(s) 1138, 1184, 1541, 1609, 1689
BsmBI CGTCTC 2 cut(s) 1541, 1609
BsmFI GGGAC 3 cut(s) 25, 338, 850
BsmI GAATGC 1 cut(s) 832
BsnI GGCC 6 cut(s) 284, 1021, 1061, 1078, 1171, 2056
BsoBI CYCGRG 1 cut(s) 321
Bsp1286I GDGCHC 1 cut(s) 2150
Bsp143I GATC 9 cut(s) 13, 193, 553, 604, 745, 1093, 1391, 1983, 2041
Bsp19I CCATGG 1 cut(s) 1375
BspACI CCGC 5 cut(s) 83, 99, 1517, 1566, 2133
BspANI GGCC 6 cut(s) 284, 1021, 1061, 1078, 1171, 2056
BspCNI CTCAG 8 cut(s) 157, 511, 526, 622, 1129, 1148, 1522, 1865
BspFNI CGCG 1 cut(s) 1566
BspHI TCATGA 1 cut(s) 1965
BspLI GGNNCC 5 cut(s) 522, 747, 1201, 1595, 2001
BspMI ACCTGC 1 cut(s) 1933
BspOI GCTAGC 1 cut(s) 2052
BspPI GGATC 7 cut(s) 201, 599, 740, 753, 1399, 1978, 2036
BsrBI CCGCTC 2 cut(s) 99, 1517
BsrDI GCAATG 1 cut(s) 195
BsrFI RCCGGY 1 cut(s) 2052
BsrI ACTGG 8 cut(s) 108, 385, 936, 1062, 1260, 1616, 1736, 1983
BssAI RCCGGY 1 cut(s) 2052
BssECI CCNNGG 7 cut(s) 286, 321, 364, 1022, 1079, 1375, 1919
BssMI GATC 9 cut(s) 13, 193, 553, 604, 745, 1093, 1391, 1983, 2041
BssNAI GTATAC 1 cut(s) 1372
BssT1I CCWWGG 4 cut(s) 286, 1022, 1375, 1919
Bst1107I GTATAC 1 cut(s) 1372
Bst2UI CCWGG 2 cut(s) 1081, 1204
Bst4CI ACNGT 7 cut(s) 218, 261, 356, 788, 1279, 1369, 1738
Bst6I CTCTTC 2 cut(s) 273, 1115
BstAPI GCANNNNNTGC 2 cut(s) 181, 619
BstBAI YACGTR 1 cut(s) 1841
BstC8I GCNNGC 8 cut(s) 70, 186, 460, 905, 1902, 2033, 2050, 2054
BstDSI CCRYGG 2 cut(s) 364, 1375
BstENI CCTNNNNNAGG 2 cut(s) 290, 1026
BstF5I GGATG 8 cut(s) 360, 775, 826, 1288, 1393, 1441, 1489, 1827
BstFNI CGCG 1 cut(s) 1566
BstHHI GCGC 1 cut(s) 2037
BstKTI GATC 9 cut(s) 16, 196, 556, 607, 748, 1096, 1394, 1986, 2044
BstMAI GTCTC 5 cut(s) 1138, 1184, 1541, 1609, 1689
BstMBI GATC 9 cut(s) 13, 193, 553, 604, 745, 1093, 1391, 1983, 2041
BstMWI GCNNNNNNNGC 6 cut(s) 181, 619, 983, 1254, 2080, 2132
BstNI CCWGG 2 cut(s) 1081, 1204
BstNSI RCATGY 1 cut(s) 188
BstSCI CCNGG 4 cut(s) 320, 321, 1079, 1202
BstSFI CTRYAG 2 cut(s) 849, 1586
BstSNI TACGTA 1 cut(s) 1841
BstUI CGCG 1 cut(s) 1566
BstV1I GCAGC 4 cut(s) 97, 102, 625, 2135
BstV2I GAAGAC 2 cut(s) 1329, 1698
BstX2I RGATCY 3 cut(s) 553, 745, 1983
BstYI RGATCY 3 cut(s) 553, 745, 1983
BstZ17I GTATAC 1 cut(s) 1372
BsuI GTATCC 1 cut(s) 1477
BsuRI GGCC 6 cut(s) 284, 1021, 1061, 1078, 1171, 2056
BtgI CCRYGG 2 cut(s) 364, 1375
BtsCI GGATG 8 cut(s) 360, 775, 826, 1288, 1393, 1441, 1489, 1827
BtsI GCAGTG 2 cut(s) 109, 2044
BtsIMutI CAGTG 7 cut(s) 109, 943, 1056, 1069, 1290, 1388, 2044
BveI ACCTGC 1 cut(s) 1933
Cac8I GCNNGC 8 cut(s) 70, 186, 460, 905, 1902, 2033, 2050, 2054
CaiI CAGNNNCTG 2 cut(s) 619, 797
CciI TCATGA 1 cut(s) 1965
CfoI GCGC 1 cut(s) 2037
Cfr10I RCCGGY 1 cut(s) 2052
Cfr13I GGNCC 7 cut(s) 283, 1059, 1077, 1169, 1200, 1206, 1999
Cfr9I CCCGGG 1 cut(s) 321
CseI GACGC 1 cut(s) 1523
Csp6I GTAC 1 cut(s) 78
CspCI CAANNNNNGTGG 2 cut(s) 1977, 2012
CviQI GTAC 1 cut(s) 78
DpnI GATC 9 cut(s) 15, 195, 555, 606, 747, 1095, 1393, 1985, 2043
DpnII GATC 9 cut(s) 13, 193, 553, 604, 745, 1093, 1391, 1983, 2041
DrdI GACNNNNNNGTC 1 cut(s) 1198
DriI GACNNNNNGTC 1 cut(s) 1702
DseDI GACNNNNNNGTC 1 cut(s) 1198
EaeI YGGCCR 1 cut(s) 2054
Eam1104I CTCTTC 2 cut(s) 273, 1115
Eam1105I GACNNNNNGTC 1 cut(s) 1702
EarI CTCTTC 2 cut(s) 273, 1115
EciI GGCGGA 1 cut(s) 2122
Eco105I TACGTA 1 cut(s) 1841
Eco130I CCWWGG 4 cut(s) 286, 1022, 1375, 1919
Eco47I GGWCC 3 cut(s) 1200, 1206, 1999
Eco57I CTGAAG 2 cut(s) 547, 2096
Eco88I CYCGRG 1 cut(s) 321
EcoNI CCTNNNNNAGG 2 cut(s) 290, 1026
EcoO109I RGGNCCY 2 cut(s) 283, 1200
EcoRII CCWGG 2 cut(s) 1079, 1202
EcoT14I CCWWGG 4 cut(s) 286, 1022, 1375, 1919
ErhI CCWWGG 4 cut(s) 286, 1022, 1375, 1919
Esp3I CGTCTC 2 cut(s) 1541, 1609
FalI AAGNNNNNCTT 2 cut(s) 270, 302
FaqI GGGAC 3 cut(s) 25, 338, 850
FbaI TGATCA 1 cut(s) 1093
FblI GTMKAC 2 cut(s) 122, 1371
Fnu4HI GCNGC 5 cut(s) 86, 100, 116, 614, 2124
FokI GGATG 8 cut(s) 347, 762, 833, 1295, 1400, 1448, 1476, 1834
Fsp4HI GCNGC 5 cut(s) 86, 100, 116, 614, 2124
FspBI CTAG 8 cut(s) 579, 840, 911, 1056, 1607, 1718, 2049, 2066
GlaI GCGC 1 cut(s) 2036
GluI GCNGC 5 cut(s) 86, 100, 116, 614, 2124
GsaI CCCAGC 2 cut(s) 46, 869
GsuI CTGGAG 2 cut(s) 1633, 2000
HaeIII GGCC 6 cut(s) 284, 1021, 1061, 1078, 1171, 2056
HapII CCGG 3 cut(s) 75, 322, 2053
HgaI GACGC 1 cut(s) 1523
HhaI GCGC 1 cut(s) 2037
Hin6I GCGC 1 cut(s) 2035
HinP1I GCGC 1 cut(s) 2035
HincII GTYRAC 1 cut(s) 123
HindII GTYRAC 1 cut(s) 123
HindIII AAGCTT 2 cut(s) 559, 2081
HinfI GANTC 8 cut(s) 63, 317, 494, 794, 929, 1192, 1915, 1962
HpaII CCGG 3 cut(s) 75, 322, 2053
HphI GGTGA 9 cut(s) 40, 767, 1165, 1518, 1531, 1658, 1818, 1897, 1957
Hpy166II GTNNAC 7 cut(s) 123, 352, 1156, 1288, 1372, 1847, 1999
Hpy188I TCNGA 8 cut(s) 277, 793, 1119, 1138, 1396, 1435, 1855, 1867
Hpy188III TCNNGA 6 cut(s) 572, 664, 679, 1694, 1966, 2066
Hpy8I GTNNAC 7 cut(s) 123, 352, 1156, 1288, 1372, 1847, 1999
Hpy99I CGWCG 2 cut(s) 124, 1623
HpyAV CCTTC 1 cut(s) 1462
HpyCH4III ACNGT 7 cut(s) 218, 261, 356, 788, 1279, 1369, 1738
HpyCH4IV ACGT 4 cut(s) 1621, 1765, 1840, 2128
HpyCH4V TGCA 8 cut(s) 163, 184, 188, 237, 830, 1542, 1942, 2095
HpyF10VI GCNNNNNNNGC 6 cut(s) 181, 619, 983, 1254, 2080, 2132
HpySE526I ACGT 4 cut(s) 1621, 1765, 1840, 2128
HspAI GCGC 1 cut(s) 2035
KroI GCCGGC 1 cut(s) 2052
KroNI GCCGGC 1 cut(s) 2054
Ksp22I TGATCA 1 cut(s) 1093
Kzo9I GATC 9 cut(s) 13, 193, 553, 604, 745, 1093, 1391, 1983, 2041
LmnI GCTCC 4 cut(s) 77, 1514, 1897, 1952
Lsp1109I GCAGC 4 cut(s) 97, 102, 625, 2135
LweI GCATC 3 cut(s) 130, 445, 698
MaeI CTAG 8 cut(s) 579, 840, 911, 1056, 1607, 1718, 2049, 2066
MaeII ACGT 4 cut(s) 1621, 1765, 1840, 2128
MaeIII GTNAC 6 cut(s) 583, 755, 1443, 1519, 1546, 1824
MalI GATC 9 cut(s) 15, 195, 555, 606, 747, 1095, 1393, 1985, 2043
MbiI CCGCTC 2 cut(s) 99, 1517
MboI GATC 9 cut(s) 13, 193, 553, 604, 745, 1093, 1391, 1983, 2041
MflI RGATCY 3 cut(s) 553, 745, 1983
MhlI GDGCHC 1 cut(s) 2150
MlyI GAGTC 1 cut(s) 1186
MmeI TCCRAC 2 cut(s) 356, 1939
MroNI GCCGGC 1 cut(s) 2052
MroXI GAANNNNTTC 2 cut(s) 295, 1782
MseI TTAA 9 cut(s) 129, 303, 467, 888, 1224, 1407, 1626, 1700, 1770
MslI CAYNNNNRTG 1 cut(s) 1494
MspA1I CMGCKG 1 cut(s) 533
MspI CCGG 3 cut(s) 75, 322, 2053
MspR9I CCNGG 4 cut(s) 322, 323, 1081, 1204
Mva1269I GAATGC 1 cut(s) 832
MvaI CCWGG 2 cut(s) 1081, 1204
MvnI CGCG 1 cut(s) 1566
MwoI GCNNNNNNNGC 6 cut(s) 181, 619, 983, 1254, 2080, 2132
NaeI GCCGGC 1 cut(s) 2054
NciI CCSGG 2 cut(s) 322, 323
NcoI CCATGG 1 cut(s) 1375
NdeII GATC 9 cut(s) 13, 193, 553, 604, 745, 1093, 1391, 1983, 2041
NgoMIV GCCGGC 1 cut(s) 2052
NheI GCTAGC 1 cut(s) 2048
NlaIV GGNNCC 5 cut(s) 522, 747, 1201, 1595, 2001
NmuCI GTSAC 4 cut(s) 755, 1519, 1546, 1824
NspI RCATGY 1 cut(s) 188
PaeI GCATGC 1 cut(s) 188
PagI TCATGA 1 cut(s) 1965
PaqCI CACCTGC 1 cut(s) 1933
PcsI WCGNNNNNNNCGW 1 cut(s) 202
PctI GAATGC 1 cut(s) 832
PdiI GCCGGC 1 cut(s) 2054
PdmI GAANNNNTTC 2 cut(s) 295, 1782
PfeI GAWTC 7 cut(s) 63, 317, 494, 794, 929, 1915, 1962
PflMI CCANNNNNTGG 1 cut(s) 1978
PfoI TCCNGGA 1 cut(s) 1202
PkrI GCNGC 5 cut(s) 87, 101, 117, 615, 2125
PleI GAGTC 1 cut(s) 1186
PpsI GAGTC 1 cut(s) 1186
Ppu21I YACGTR 1 cut(s) 1841
PpuMI RGGWCCY 1 cut(s) 1200
PshBI ATTAAT 1 cut(s) 1626
PsiI TTATAA 2 cut(s) 674, 1790
Psp5II RGGWCCY 1 cut(s) 1200
Psp6I CCWGG 2 cut(s) 1079, 1202
PspFI CCCAGC 2 cut(s) 42, 865
PspGI CCWGG 2 cut(s) 1079, 1202
PspN4I GGNNCC 5 cut(s) 522, 747, 1201, 1595, 2001
PspPI GGNCC 7 cut(s) 283, 1059, 1077, 1169, 1200, 1206, 1999
PspPPI RGGWCCY 1 cut(s) 1200
PstNI CAGNNNCTG 2 cut(s) 619, 797
PsuI RGATCY 3 cut(s) 553, 745, 1983
PvuII CAGCTG 1 cut(s) 533
RsaI GTAC 1 cut(s) 79
RsaNI GTAC 1 cut(s) 78
RseI CAYNNNNRTG 1 cut(s) 1494
SalI GTCGAC 1 cut(s) 121
SaqAI TTAA 9 cut(s) 129, 303, 467, 888, 1224, 1407, 1626, 1700, 1770
SatI GCNGC 5 cut(s) 86, 100, 116, 614, 2124
Sau3AI GATC 9 cut(s) 13, 193, 553, 604, 745, 1093, 1391, 1983, 2041
Sau96I GGNCC 7 cut(s) 283, 1059, 1077, 1169, 1200, 1206, 1999
SchI GAGTC 1 cut(s) 1186
ScrFI CCNGG 4 cut(s) 322, 323, 1081, 1204
SduI GDGCHC 1 cut(s) 2150
SfaNI GCATC 3 cut(s) 130, 445, 698
SfcI CTRYAG 2 cut(s) 849, 1586
SinI GGWCC 3 cut(s) 1200, 1206, 1999
SmaI CCCGGG 1 cut(s) 323
SmiMI CAYNNNNRTG 1 cut(s) 1494
SmlI CTYRAG 3 cut(s) 26, 133, 1194
SmoI CTYRAG 3 cut(s) 26, 133, 1194
SnaBI TACGTA 1 cut(s) 1841
SphI GCATGC 1 cut(s) 188
SsiI CCGC 5 cut(s) 83, 99, 1517, 1566, 2133
SspI AATATT 1 cut(s) 1430
SspMI CTAG 8 cut(s) 579, 840, 911, 1056, 1607, 1718, 2049, 2066
StyD4I CCNGG 4 cut(s) 320, 321, 1079, 1202
StyI CCWWGG 4 cut(s) 286, 1022, 1375, 1919
TaaI ACNGT 7 cut(s) 218, 261, 356, 788, 1279, 1369, 1738
TaiI ACGT 4 cut(s) 1624, 1768, 1843, 2131
TaqI TCGA 8 cut(s) 6, 122, 167, 692, 716, 1486, 1693, 2076
TauI GCSGC 1 cut(s) 102
TfiI GAWTC 7 cut(s) 63, 317, 494, 794, 929, 1915, 1962
Tru1I TTAA 9 cut(s) 129, 303, 467, 888, 1224, 1407, 1626, 1700, 1770
Tru9I TTAA 9 cut(s) 129, 303, 467, 888, 1224, 1407, 1626, 1700, 1770
TscAI CASTG 7 cut(s) 109, 943, 1056, 1069, 1297, 1388, 2044
TseFI GTSAC 4 cut(s) 755, 1519, 1546, 1824
TseI GCWGC 4 cut(s) 85, 115, 613, 2123
Tsp45I GTSAC 4 cut(s) 755, 1519, 1546, 1824
TspGWI ACGGA 1 cut(s) 768
TspMI CCCGGG 1 cut(s) 321
TspRI CASTG 7 cut(s) 109, 943, 1056, 1069, 1297, 1388, 2044
Van91I CCANNNNNTGG 1 cut(s) 1978
VpaK11BI GGWCC 3 cut(s) 1200, 1206, 1999
VspI ATTAAT 1 cut(s) 1626
XagI CCTNNNNNAGG 2 cut(s) 290, 1026
XapI RAATTY 4 cut(s) 593, 720, 1146, 1832
XbaI TCTAGA 1 cut(s) 2065
XceI RCATGY 1 cut(s) 188
XcmI CCANNNNNNNNNTGG 1 cut(s) 1267
XmaI CCCGGG 1 cut(s) 321
XmiI GTMKAC 2 cut(s) 122, 1371
XmnI GAANNNNTTC 2 cut(s) 295, 1782
XspI CTAG 8 cut(s) 579, 840, 911, 1056, 1607, 1718, 2049, 2066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.