Rroxscaffold_5G00367310

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000660
Physical Location & Seq
Forward (+)
49150595 .. 49156631
6037 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00367310.1

Sequence Viewer

Length: 1959 bp
ATGGTCGAAAGTGATCTCGCCCACCTCAAGCAAGAAATGTCCCAGCTCACCAGCCACCGCCGAATCGCCAGCTCCAGTACTCCGCAGCCCAAAACGAGAGGCAGTGCCAGTCGGCTGTCGTCGACTTTTAACTTGAGTGATGCTCAGTATCTCAACATACTGCAGTCGATTGGGCAGTCTGTGCATGCAATGGATCGTAGTGGTCGTATAATCTACTGGAACAGAAGTGCTGAAAAACTGTATGGTTATTCTTCGGAAGAGGCCCTTGGAAAGGTTTCCATTAAGCTCATAGTAGATTCCCGGGACTTTGGTGTAGCATATAATATAGTCCACCGTGTCATCCACGGGCAGAGTTGGACTGGGCAGTTCCCTGTAAAGCACAAATCGGGGGCGAGGTTTTCAGTCATTACAACAAATACTCCATTCTATGATGATGCTGGCAGTTTAATTGGGGTTGTTGCTACATCAACTGATTCACAGCCCTTTTATGAAATGAAGGTTCCAATGTCAGCTGAGAAGCAACCACAACAAGATCTAAGCTTCAGCAGTGGTGGGGCTAGTGTTACAACTAAATTCGGTGCTGATCCTCAGCAGCCTCTGCCAAAAGCAGTTTTATCAAAACTAACAGACTTGGCATCGAAGATGAGTTTCAAAGTCAAGTCAAAAATTCAGGCAAGAGAAAACAACATGGATCCCGAAGGTGACAGCGAAGATAGTCATCATCCGTTTCACAGTTCAGATTCTGTCATATCTGCAAATAGGGAGGATGGGAATGCAAGTGGGACTAGCACAGTTATAGGAACTATACCCCCAGCAACTCCTTTTGGTGTATTTAACCATGTTGAGAGCAAGCCTCTAGGAGAACCCTCCATAGATTCTGCCAGTGAGAGCAAAGAAAAATCTTCAATTCACAAGCTAATTGCCTCAAAGGCAGAAGCATGGATTAGTAAGCAGAAGATATCATGGCCTTGGAAAGGAAATGAGCGAGATGGGTCAGTGGCTAGGGCCAGTGGTTTTGTTTGGCCCTGGTTTCATAATGATCAAGTCAATGTTGTCATTCCTCAGAAGATTTATTGTGTCTCAGAACCAGAAATTCAGGTGAGCGAAAGTAATGGGCCAACAAATAATAATGATGAGACTCAAGGATCCTGGACCTCTCCATTGGATATTAACAGCATAAGCCGTGCCAGCCTTAATAGGACCAGTAGTGCTTTGGCTACGGTGGACGTGGACACTGATTGCTTGGATTATGAAATCTTATGGGAAGACATGATAATTGGAAGACAGATTGGGCAAGGTAATAATATTTCTAGTGACGGGAGCATGCTGGATGTTGCCGTTAAGGTATTCTCCAAGTTAGAATATTCGGATGATTTGTTACTTTCCTTCAAACAAGAGGTATCTCTAATGAAAAGGCTTCGACATCCAAATGTTTTGCTTTTCATGGGAGCGGTGACTTCACCTGAGCGTCTCTGCATTGTCACAGAGTTCCTCCCACGTGGAAGTTTGTATCAAATACTACAGAGGAACCCATCAAAACTAGACTGGAGACGACGTGTTAATATGGCTATGGATATAGCATGGGGCATGAATTATCTTCACCATTTCAACCCACCAATCATTCATCGAGACTTAAAGTCTTCAAATCTCCTAGTTGATAAGAACTGGACTGTCAAGCCTCAATGGATGTCACCAGAATTTCTACGTAGTGAACCCTCGGATGAGAAGTCTGACATATATAGCTACGGGGTGATATTGTGGGAGCTTGCTACTGAGAAGATTCCTTGGGATCATCTAAACCCAATGCAGGATTTATTTTTCCTTCTCACTAGTGATCCAGCTAGCCGGCCAACATTTCTAGAACTGCTCGACAAGCTTAGAGTGTTGCATAGACAATATAATCTCCAGTTCAAAGCAGCGCGTTCCGCCTCTAGTGAGAGCACCCAAAAGGAATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS PAS_9 Pkinase_Tyr

Protein Analysis

652

Amino Acids

72.95

Weight (kDa)

6.33

Isoelectric Point (pI)

47.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS PF00989 49 - 155 7.6e-11 PAS fold
PAS_4 PF08448 55 - 159 1.1e-08 PAS fold
PAS_9 PF13426 65 - 158 6.5e-08 PAS domain
PAS_3 PF08447 70 - 155 1.7e-08 PAS fold
PK_Tyr_Ser-Thr PF07714 437 - 559 5.2e-28 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 441 - 559 4.1e-20 Protein kinase domain
Pkinase PF00069 557 - 601 1.2e-07 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 559 - 605 4.2e-08 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1451
AccI GTMKAC 1 cut(s) 122
AccII CGCG 1 cut(s) 1921
AciI CCGC 4 cut(s) 58, 83, 1451, 1926
AclWI GGATC 8 cut(s) 201, 578, 686, 699, 1140, 1153, 1797, 1829
AcoI YGGCCR 1 cut(s) 1847
AcsI RAATTY 4 cut(s) 572, 666, 1092, 1697
AcuI CTGAAG 1 cut(s) 526
AcvI CACGTG 1 cut(s) 1499
AfaI GTAC 1 cut(s) 79
AfiI CCNNNNNNNGG 3 cut(s) 271, 974, 1807
AflIII ACRYGT 1 cut(s) 1555
AgsI TTSAA 6 cut(s) 652, 906, 1390, 1609, 1644, 1912
AhdI GACNNNNNGTC 1 cut(s) 1636
AhlI ACTAGT 1 cut(s) 1829
AjiI CACGTC 2 cut(s) 1228, 1556
AjnI CCWGG 2 cut(s) 1025, 1148
AjuI GAANNNNNNNTTGG 2 cut(s) 249, 281
Alw21I GWGCWC 1 cut(s) 1943
Alw26I GTCTC 5 cut(s) 1084, 1130, 1475, 1543, 1623
AlwI GGATC 8 cut(s) 201, 578, 686, 699, 1140, 1153, 1797, 1829
AlwNI CAGNNNCTG 2 cut(s) 598, 743
Ama87I CYCGRG 1 cut(s) 300
AoxI GGCC 6 cut(s) 261, 965, 1005, 1022, 1115, 1847
ApeKI GCWGC 3 cut(s) 85, 592, 1916
ApoI RAATTY 4 cut(s) 572, 666, 1092, 1697
Asp700I GAANNNNTTC 1 cut(s) 274
AspLEI GCGC 1 cut(s) 1921
AspS9I GGNCC 6 cut(s) 262, 1005, 1023, 1115, 1152, 1200
AsuC2I CCSGG 2 cut(s) 301, 302
AsuHPI GGTGA 8 cut(s) 40, 713, 1111, 1452, 1465, 1592, 1683, 1762
AsuNHI GCTAGC 1 cut(s) 1841
AvaI CYCGRG 1 cut(s) 300
AvaII GGWCC 2 cut(s) 1152, 1200
BamHI GGATCC 2 cut(s) 691, 1145
BbrPI CACGTG 1 cut(s) 1499
BbsI GAAGAC 3 cut(s) 1272, 1288, 1632
Bbv12I GWGCWC 1 cut(s) 1943
BbvCI CCTCAGC 1 cut(s) 588
BbvI GCAGC 3 cut(s) 97, 604, 1928
BccI CCATC 3 cut(s) 761, 983, 1540
BceAI ACGGC 2 cut(s) 1167, 1322
BciT130I CCWGG 2 cut(s) 1027, 1150
BclI TGATCA 1 cut(s) 1039
BcnI CCSGG 2 cut(s) 301, 302
BcoDI GTCTC 5 cut(s) 1084, 1130, 1475, 1543, 1623
BcuI ACTAGT 1 cut(s) 1829
BfmI CTRYAG 2 cut(s) 161, 1520
BglI GCCNNNNNGGC 1 cut(s) 929
BglII AGATCT 1 cut(s) 532
BisI GCNGC 3 cut(s) 86, 593, 1917
BlsI GCNGC 3 cut(s) 87, 594, 1918
BmcAI AGTACT 1 cut(s) 79
Bme1390I CCNGG 4 cut(s) 301, 302, 1027, 1150
Bme18I GGWCC 2 cut(s) 1152, 1200
BmeRI GACNNNNNGTC 1 cut(s) 1636
BmeT110I CYCGRG 1 cut(s) 300
BmgBI CACGTC 2 cut(s) 1228, 1556
BmgT120I GGNCC 6 cut(s) 262, 1005, 1023, 1115, 1152, 1200
BmiI GGNNCC 4 cut(s) 501, 693, 1147, 1529
BmrFI CCNGG 4 cut(s) 301, 302, 1027, 1150
BmrI ACTGGG 1 cut(s) 369
BmsI GCATC 3 cut(s) 130, 424, 644
BmtI GCTAGC 1 cut(s) 1845
BmuI ACTGGG 1 cut(s) 369
BpiI GAAGAC 3 cut(s) 1272, 1288, 1632
BplI GAGNNNNNCTC 4 cut(s) 127, 159, 838, 870
BpmI CTGGAG 3 cut(s) 58, 1567, 1889
Bpu10I CCTNAGC 2 cut(s) 588, 1464
BpuEI CTTGAG 3 cut(s) 11, 154, 1125
BpuMI CCSGG 2 cut(s) 301, 302
BsaAI YACGTR 2 cut(s) 1499, 1706
BsaBI GATNNNNATC 1 cut(s) 717
BsaJI CCNNGG 7 cut(s) 265, 300, 343, 968, 1025, 1716, 1784
BsaXI ACNNNNNCTCC 4 cut(s) 403, 433, 1540, 1570
Bsc4I CCNNNNNNNGG 3 cut(s) 271, 974, 1807
Bse118I RCCGGY 1 cut(s) 1845
Bse3DI GCAATG 1 cut(s) 195
Bse8I GATNNNNATC 1 cut(s) 717
BseBI CCWGG 2 cut(s) 1027, 1150
BseDI CCNNGG 7 cut(s) 265, 300, 343, 968, 1025, 1716, 1784
BseGI GGATG 8 cut(s) 339, 721, 772, 1336, 1375, 1423, 1692, 1726
BseJI GATNNNNATC 1 cut(s) 717
BseLI CCNNNNNNNGG 3 cut(s) 271, 974, 1807
BseMI GCAATG 1 cut(s) 195
BseMII CTCAG 7 cut(s) 158, 504, 602, 1076, 1095, 1455, 1764
BseXI GCAGC 3 cut(s) 97, 604, 1928
BseYI CCCAGC 2 cut(s) 42, 811
Bsh1236I CGCG 1 cut(s) 1921
BshFI GGCC 6 cut(s) 263, 967, 1007, 1024, 1117, 1849
BsiHKAI GWGCWC 1 cut(s) 1943
BsiHKCI CYCGRG 1 cut(s) 300
BsiSI CCGG 2 cut(s) 301, 1846
BslFI GGGAC 3 cut(s) 25, 317, 796
BslI CCNNNNNNNGG 3 cut(s) 271, 974, 1807
BsmAI GTCTC 5 cut(s) 1084, 1130, 1475, 1543, 1623
BsmBI CGTCTC 2 cut(s) 1475, 1543
BsmFI GGGAC 3 cut(s) 25, 317, 796
BsmI GAATGC 1 cut(s) 778
BsnI GGCC 6 cut(s) 263, 967, 1007, 1024, 1117, 1849
BsoBI CYCGRG 1 cut(s) 300
Bsp1286I GDGCHC 1 cut(s) 1943
Bsp143I GATC 9 cut(s) 13, 193, 532, 583, 691, 1039, 1145, 1789, 1834
BspACI CCGC 4 cut(s) 58, 83, 1451, 1926
BspANI GGCC 6 cut(s) 263, 967, 1007, 1024, 1117, 1849
BspCNI CTCAG 7 cut(s) 157, 505, 601, 1075, 1094, 1456, 1765
BspFNI CGCG 1 cut(s) 1921
BspLI GGNNCC 4 cut(s) 501, 693, 1147, 1529
BspMAI CTGCAG 1 cut(s) 165
BspOI GCTAGC 1 cut(s) 1845
BspPI GGATC 8 cut(s) 201, 578, 686, 699, 1140, 1153, 1797, 1829
BsrBI CCGCTC 1 cut(s) 1451
BsrDI GCAATG 1 cut(s) 195
BsrFI RCCGGY 1 cut(s) 1845
BssAI RCCGGY 1 cut(s) 1845
BssECI CCNNGG 7 cut(s) 265, 300, 343, 968, 1025, 1716, 1784
BssMI GATC 9 cut(s) 13, 193, 532, 583, 691, 1039, 1145, 1789, 1834
BssT1I CCWWGG 3 cut(s) 265, 968, 1784
Bst2UI CCWGG 2 cut(s) 1027, 1150
Bst4CI ACNGT 6 cut(s) 240, 335, 734, 793, 1222, 1672
Bst6I CTCTTC 1 cut(s) 252
BstAPI GCANNNNNTGC 2 cut(s) 181, 598
BstBAI YACGTR 2 cut(s) 1499, 1706
BstC8I GCNNGC 9 cut(s) 70, 186, 439, 851, 1189, 1325, 1767, 1843, 1847
BstDEI CTNAG 9 cut(s) 144, 513, 536, 588, 1062, 1081, 1464, 1773, 1877
BstDSI CCRYGG 1 cut(s) 343
BstENI CCTNNNNNAGG 2 cut(s) 269, 972
BstF5I GGATG 8 cut(s) 339, 721, 772, 1336, 1375, 1423, 1692, 1726
BstFNI CGCG 1 cut(s) 1921
BstHHI GCGC 1 cut(s) 1921
BstKTI GATC 9 cut(s) 16, 196, 535, 586, 694, 1042, 1148, 1792, 1837
BstMAI GTCTC 5 cut(s) 1084, 1130, 1475, 1543, 1623
BstMBI GATC 9 cut(s) 13, 193, 532, 583, 691, 1039, 1145, 1789, 1834
BstMWI GCNNNNNNNGC 6 cut(s) 181, 598, 929, 1188, 1873, 1925
BstNI CCWGG 2 cut(s) 1027, 1150
BstNSI RCATGY 2 cut(s) 188, 1327
BstSCI CCNGG 4 cut(s) 299, 300, 1025, 1148
BstSFI CTRYAG 2 cut(s) 161, 1520
BstSNI TACGTA 1 cut(s) 1706
BstUI CGCG 1 cut(s) 1921
BstV1I GCAGC 3 cut(s) 97, 604, 1928
BstV2I GAAGAC 3 cut(s) 1272, 1288, 1632
BstX2I RGATCY 3 cut(s) 532, 691, 1145
BstYI RGATCY 3 cut(s) 532, 691, 1145
BsuRI GGCC 6 cut(s) 263, 967, 1007, 1024, 1117, 1849
BtgI CCRYGG 1 cut(s) 343
BtrI CACGTC 2 cut(s) 1228, 1556
BtsCI GGATG 8 cut(s) 339, 721, 772, 1336, 1375, 1423, 1692, 1726
BtsI GCAGTG 2 cut(s) 109, 553
BtsIMutI CAGTG 6 cut(s) 109, 553, 889, 1002, 1015, 1233
Cac8I GCNNGC 9 cut(s) 70, 186, 439, 851, 1189, 1325, 1767, 1843, 1847
CaiI CAGNNNCTG 2 cut(s) 598, 743
CfoI GCGC 1 cut(s) 1921
Cfr10I RCCGGY 1 cut(s) 1845
Cfr13I GGNCC 6 cut(s) 262, 1005, 1023, 1115, 1152, 1200
Cfr9I CCCGGG 1 cut(s) 300
CseI GACGC 1 cut(s) 1457
Csp6I GTAC 1 cut(s) 78
CviQI GTAC 1 cut(s) 78
DdeI CTNAG 9 cut(s) 144, 513, 536, 588, 1062, 1081, 1464, 1773, 1877
DpnI GATC 9 cut(s) 15, 195, 534, 585, 693, 1041, 1147, 1791, 1836
DpnII GATC 9 cut(s) 13, 193, 532, 583, 691, 1039, 1145, 1789, 1834
DriI GACNNNNNGTC 1 cut(s) 1636
EaeI YGGCCR 1 cut(s) 1847
Eam1104I CTCTTC 1 cut(s) 252
Eam1105I GACNNNNNGTC 1 cut(s) 1636
EarI CTCTTC 1 cut(s) 252
EciI GGCGGA 1 cut(s) 1915
Eco105I TACGTA 1 cut(s) 1706
Eco130I CCWWGG 3 cut(s) 265, 968, 1784
Eco32I GATATC 1 cut(s) 960
Eco47I GGWCC 2 cut(s) 1152, 1200
Eco57I CTGAAG 1 cut(s) 526
Eco72I CACGTG 1 cut(s) 1499
Eco88I CYCGRG 1 cut(s) 300
EcoNI CCTNNNNNAGG 2 cut(s) 269, 972
EcoO109I RGGNCCY 1 cut(s) 262
EcoRII CCWGG 2 cut(s) 1025, 1148
EcoRV GATATC 1 cut(s) 960
EcoT14I CCWWGG 3 cut(s) 265, 968, 1784
ErhI CCWWGG 3 cut(s) 265, 968, 1784
Esp3I CGTCTC 2 cut(s) 1475, 1543
FalI AAGNNNNNCTT 2 cut(s) 249, 281
FaqI GGGAC 3 cut(s) 25, 317, 796
FbaI TGATCA 1 cut(s) 1039
FblI GTMKAC 1 cut(s) 122
Fnu4HI GCNGC 3 cut(s) 86, 593, 1917
FokI GGATG 8 cut(s) 326, 708, 779, 1343, 1382, 1410, 1699, 1733
Fsp4HI GCNGC 3 cut(s) 86, 593, 1917
GlaI GCGC 1 cut(s) 1920
GluI GCNGC 3 cut(s) 86, 593, 1917
GsaI CCCAGC 2 cut(s) 46, 815
GsuI CTGGAG 3 cut(s) 58, 1567, 1889
HaeIII GGCC 6 cut(s) 263, 967, 1007, 1024, 1117, 1849
HapII CCGG 2 cut(s) 301, 1846
HgaI GACGC 1 cut(s) 1457
HhaI GCGC 1 cut(s) 1921
Hin6I GCGC 1 cut(s) 1919
HinP1I GCGC 1 cut(s) 1919
HincII GTYRAC 1 cut(s) 123
HindII GTYRAC 1 cut(s) 123
HindIII AAGCTT 2 cut(s) 538, 1874
HinfI GANTC 7 cut(s) 63, 296, 473, 740, 875, 1138, 1780
HpaII CCGG 2 cut(s) 301, 1846
HphI GGTGA 8 cut(s) 40, 713, 1111, 1452, 1465, 1592, 1683, 1762
Hpy166II GTNNAC 5 cut(s) 123, 331, 1225, 1231, 1712
Hpy188I TCNGA 7 cut(s) 256, 739, 1065, 1084, 1369, 1720, 1732
Hpy188III TCNNGA 3 cut(s) 695, 1628, 1859
Hpy8I GTNNAC 5 cut(s) 123, 331, 1225, 1231, 1712
Hpy99I CGWCG 2 cut(s) 124, 1557
HpyAV CCTTC 4 cut(s) 490, 692, 1396, 1832
HpyCH4III ACNGT 6 cut(s) 240, 335, 734, 793, 1222, 1672
HpyCH4IV ACGT 4 cut(s) 1227, 1498, 1555, 1705
HpyCH4V TGCA 8 cut(s) 163, 184, 188, 755, 776, 1476, 1807, 1888
HpyF10VI GCNNNNNNNGC 6 cut(s) 181, 598, 929, 1188, 1873, 1925
HpyF3I CTNAG 9 cut(s) 144, 513, 536, 588, 1062, 1081, 1464, 1773, 1877
HpySE526I ACGT 4 cut(s) 1227, 1498, 1555, 1705
HspAI GCGC 1 cut(s) 1919
KroI GCCGGC 1 cut(s) 1845
KroNI GCCGGC 1 cut(s) 1847
Ksp22I TGATCA 1 cut(s) 1039
Kzo9I GATC 9 cut(s) 13, 193, 532, 583, 691, 1039, 1145, 1789, 1834
LmnI GCTCC 4 cut(s) 77, 1320, 1448, 1762
Lsp1109I GCAGC 3 cut(s) 97, 604, 1928
LweI GCATC 3 cut(s) 130, 424, 644
MaeII ACGT 4 cut(s) 1227, 1498, 1555, 1705
MaeIII GTNAC 7 cut(s) 562, 701, 1313, 1377, 1453, 1480, 1689
MalI GATC 9 cut(s) 15, 195, 534, 585, 693, 1041, 1147, 1791, 1836
MbiI CCGCTC 1 cut(s) 1451
MboI GATC 9 cut(s) 13, 193, 532, 583, 691, 1039, 1145, 1789, 1834
MflI RGATCY 3 cut(s) 532, 691, 1145
MhlI GDGCHC 1 cut(s) 1943
MlyI GAGTC 1 cut(s) 1132
MmeI TCCRAC 1 cut(s) 335
MroNI GCCGGC 1 cut(s) 1845
MroXI GAANNNNTTC 1 cut(s) 274
MseI TTAA 9 cut(s) 129, 282, 446, 834, 1170, 1194, 1341, 1560, 1634
MslI CAYNNNNRTG 1 cut(s) 1428
MspA1I CMGCKG 1 cut(s) 512
MspI CCGG 2 cut(s) 301, 1846
MspR9I CCNGG 4 cut(s) 301, 302, 1027, 1150
Mva1269I GAATGC 1 cut(s) 778
MvaI CCWGG 2 cut(s) 1027, 1150
MvnI CGCG 1 cut(s) 1921
MwoI GCNNNNNNNGC 6 cut(s) 181, 598, 929, 1188, 1873, 1925
NaeI GCCGGC 1 cut(s) 1847
NciI CCSGG 2 cut(s) 301, 302
NdeII GATC 9 cut(s) 13, 193, 532, 583, 691, 1039, 1145, 1789, 1834
NgoMIV GCCGGC 1 cut(s) 1845
NheI GCTAGC 1 cut(s) 1841
NlaIV GGNNCC 4 cut(s) 501, 693, 1147, 1529
NmuCI GTSAC 5 cut(s) 701, 1313, 1453, 1480, 1689
NspI RCATGY 2 cut(s) 188, 1327
PaeI GCATGC 2 cut(s) 188, 1327
PcsI WCGNNNNNNNCGW 1 cut(s) 202
PctI GAATGC 1 cut(s) 778
PdiI GCCGGC 1 cut(s) 1847
PdmI GAANNNNTTC 1 cut(s) 274
PfeI GAWTC 6 cut(s) 63, 296, 473, 740, 875, 1780
PfoI TCCNGGA 1 cut(s) 1148
PkrI GCNGC 3 cut(s) 87, 594, 1918
PleI GAGTC 1 cut(s) 1132
PmaCI CACGTG 1 cut(s) 1499
PmlI CACGTG 1 cut(s) 1499
PpsI GAGTC 1 cut(s) 1132
Ppu21I YACGTR 2 cut(s) 1499, 1706
Psp6I CCWGG 2 cut(s) 1025, 1148
PspCI CACGTG 1 cut(s) 1499
PspFI CCCAGC 2 cut(s) 42, 811
PspGI CCWGG 2 cut(s) 1025, 1148
PspN4I GGNNCC 4 cut(s) 501, 693, 1147, 1529
PspPI GGNCC 6 cut(s) 262, 1005, 1023, 1115, 1152, 1200
PstI CTGCAG 1 cut(s) 165
PstNI CAGNNNCTG 2 cut(s) 598, 743
PsuI RGATCY 3 cut(s) 532, 691, 1145
PvuII CAGCTG 1 cut(s) 512
RsaI GTAC 1 cut(s) 79
RsaNI GTAC 1 cut(s) 78
RseI CAYNNNNRTG 1 cut(s) 1428
SalI GTCGAC 1 cut(s) 121
SaqAI TTAA 9 cut(s) 129, 282, 446, 834, 1170, 1194, 1341, 1560, 1634
SatI GCNGC 3 cut(s) 86, 593, 1917
Sau3AI GATC 9 cut(s) 13, 193, 532, 583, 691, 1039, 1145, 1789, 1834
Sau96I GGNCC 6 cut(s) 262, 1005, 1023, 1115, 1152, 1200
ScaI AGTACT 1 cut(s) 79
SchI GAGTC 1 cut(s) 1132
ScrFI CCNGG 4 cut(s) 301, 302, 1027, 1150
SduI GDGCHC 1 cut(s) 1943
SfaNI GCATC 3 cut(s) 130, 424, 644
SfcI CTRYAG 2 cut(s) 161, 1520
SinI GGWCC 2 cut(s) 1152, 1200
SmaI CCCGGG 1 cut(s) 302
SmiMI CAYNNNNRTG 1 cut(s) 1428
SmlI CTYRAG 3 cut(s) 26, 133, 1140
SmoI CTYRAG 3 cut(s) 26, 133, 1140
SnaBI TACGTA 1 cut(s) 1706
SpeI ACTAGT 1 cut(s) 1829
SphI GCATGC 2 cut(s) 188, 1327
SsiI CCGC 4 cut(s) 58, 83, 1451, 1926
SspI AATATT 2 cut(s) 1306, 1364
StyD4I CCNGG 4 cut(s) 299, 300, 1025, 1148
StyI CCWWGG 3 cut(s) 265, 968, 1784
TaaI ACNGT 6 cut(s) 240, 335, 734, 793, 1222, 1672
TaiI ACGT 4 cut(s) 1230, 1501, 1558, 1708
TaqI TCGA 7 cut(s) 6, 122, 167, 638, 1420, 1627, 1869
TatI WGTACW 1 cut(s) 77
TfiI GAWTC 6 cut(s) 63, 296, 473, 740, 875, 1780
Tru1I TTAA 9 cut(s) 129, 282, 446, 834, 1170, 1194, 1341, 1560, 1634
Tru9I TTAA 9 cut(s) 129, 282, 446, 834, 1170, 1194, 1341, 1560, 1634
TscAI CASTG 6 cut(s) 109, 553, 889, 1002, 1015, 1240
TseFI GTSAC 5 cut(s) 701, 1313, 1453, 1480, 1689
TseI GCWGC 3 cut(s) 85, 592, 1916
Tsp45I GTSAC 5 cut(s) 701, 1313, 1453, 1480, 1689
TspDTI ATGAA 8 cut(s) 504, 509, 1022, 1266, 1424, 1432, 1604, 1613
TspGWI ACGGA 1 cut(s) 714
TspMI CCCGGG 1 cut(s) 300
TspRI CASTG 6 cut(s) 109, 553, 889, 1002, 1015, 1240
VpaK11BI GGWCC 2 cut(s) 1152, 1200
XagI CCTNNNNNAGG 2 cut(s) 269, 972
XapI RAATTY 4 cut(s) 572, 666, 1092, 1697
XbaI TCTAGA 1 cut(s) 1858
XceI RCATGY 2 cut(s) 188, 1327
XcmI CCANNNNNNNNNTGG 1 cut(s) 1210
XmaI CCCGGG 1 cut(s) 300
XmiI GTMKAC 1 cut(s) 122
XmnI GAANNNNTTC 1 cut(s) 274
ZrmI AGTACT 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.