MD13G1153300.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000660
Physical Location & Seq
Reverse (-)
12010036 .. 12015987
5952 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1153300.v1.1.491

Sequence Viewer

Length: 2340 bp
ATGGATACTCCGCCGGCGGAGGAGCTTCTGAAGAAGATCCAAGTGCTGGAAGCGGGTCAGGCCGACCTCAAGAAGGAGATGACCAAGCTCAGGCACTCCGAGGATGTCAAATCAGAGCACCAGAGGGCCCACTCCGTCTCGCCGCAGCGCTCAAGGTTCTCGTCTGTGCCCAGAAGGAGAGTCGCTGGGGGCGGCGGTGCTGCTGGTAATGGAGGTCACGATGCGGCGGCTTGGAAGAGAGGGTCTTCTTCATTTCGGCATTCCTCGCCGCTTCAGAGGGAGAGCCGCAGCCATGATCCTCCCACTGGAGGCAGTGGCGGCGGCGGCGGCAGTGGAGGAAGCAGTAGCGCCGGACCTTCAGCTGTGAACTTTACTGACAGGCAGTATCTGAACATATTGCAGTCAATTGGGCAATCTGTGCATATATTTGATATCAATGGGCTTATAATTTACTGGAACAAAACTGCTGAAAATTTATATGGTTATTCTGCGGCTGAAGCACTTGGACATAGTCCCATTGAGCTCTTAGTAGATCCTCAAGACTATGCTGTAGCAAACACTATAATCCATCGTGTCAGCAAGGGGGAGAGCTGGACTGGGCAGTTCCCTGTCAAGAACAAAGCTGGGGAGAGGTTTACGGCCGTTGTAACAGATACTCCATTCTATGATGATGATGGCACTTTTATTGGGATTATTTGCGTGTCAAGTGATATCCGGCCCTTTCGAGAAATTAAGATTCCAATGTCAGGAGTAAAGCCCCCAGAATCAGATTCAAGCTACAGCCGGTCTAGAGCTCGTGTTACTGCTAAACTTGGCCTTGATCCTCAGCAGCCTCTACAAAATGCCATTGCATCCAAAATAACAAATTTGGCATCCAAAGTGAGCAACAAAGTAAAGTCAAAAATTCGGGTGGGAGAAAACAACATAGATCACGAAGGTGAGAGTGGTTGTCATCATTCTGATCATGGTTCAGATTGTGTTCTCTCTGATCATAGGGAGGATGCGAATTCAAGTGGGGCTAGCACACCTAGAGGAGATTTGCCCCTATCTCCTTTTGGAGTATTCTCCAATATTGATGAGAAGTCTCCAGGAAAGCCCTACAAAGATTCTGGTGATGAGAGTGAAGGAAAACCTTCGATCCAGATGATGATATCCTCAAAAGCAGAATCATGGATGGGTAAGAAAGGGATAACATGGCCATGGAAAGGAAATGGGCGAGAAGGGCAAGAGGCTAAGAATACTCATTTTGTTTGGCCTTGGTTGCATAATGAACATGAGAATGATTCAGCTCATCAGAAGAATTATTTTGGTTCAAAACCAGAAAGTCGGGTGAATGAAAATAACCGAACTCCAAATAATGAGGCCACAGGATCCTGGACCTCCTCATTTAATGTTAACAGCACAAGCAGTGCCAGCAGCTGTGGAAGTACTAGCAGCAGTGCTGTGAATAAGGTGGATGTAGACACTGACAGCCTGGACTACGAAATCCTATGGGAAGATTTGATAATTGGAGAACAAGTTGGGCAAGGATCATGGGGAACCGTATACCATGGTCTGTGGTATGGATCAGATGTTGCTGTCAAGGTATTCTCCAGGCAAGAATATTCAGAAGATGTGATATTTTCCTTCAGACAAGAGGTGTCTCTCATGAAAAGGCTTCGACATCCAAATGTTCTGCTTTTTATGGGAGCTGTGACTTCACCTCAGCGTCTCTGCATCGTAACTGAATTCCTCCCTCGTGGAAGTTTGTTTCGCTTATTACAAAGGAACACATCAAAACTAGACTGGAGGCGACGTGTTCTTATGGCGATAGATATAGCACGAGGCATGAATTATCTTCATCATTTCAACCCACCTATCATTCATCGAGATCTGAAGTCTTCAAATCTCCTAGTTGATAGGAACTGGACGGTCAAGGTTGGTGATTTTGGTCTTTCACGTCTTAAGCACGAAACATTTCTCACAACCAAGACCGGGAAGGGCACGCCTCAATGGATGGCACCAGAAGTTCTACGTAATGAACCCTCAGACGAGAAGTCTGATATATACAGCTATGGGGTGATATTGTGGGAGCTTGCTACTGAGAAGATCCCTTGGGATAATCTGAACTCAATGCAGGTGATTGGAGCTGTAGGGTTCATGGACCAAAGGTTAGAGATCCCAAAGGATGTGGACCCACAATGGACTTCTTTGATTGAGAGCTGCTGGCAGAGTGATGCAGCTGCCAGGCCAACATTTCAAGAACTGCTGGAGAGGCTCAGAGACCTGCAGAGACAGTACAATCTTCAGTTCCAAGCAGCGCGTACCGCCACGGGTGATAACGCCCCGAAAGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS PAS_9 Pkinase_Tyr

Protein Analysis

780

Amino Acids

86.37

Weight (kDa)

6.68

Isoelectric Point (pI)

46.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS PF00989 128 - 238 5.2e-14 PAS fold
PAS_4 PF08448 134 - 242 3.7e-10 PAS fold
PAS_9 PF13426 145 - 238 8.3e-11 PAS domain
PAS_3 PF08447 149 - 234 8e-08 PAS fold
PK_Tyr_Ser-Thr PF07714 503 - 753 1e-66 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 504 - 751 2e-55 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 446
AarI CACCTGC 1 cut(s) 2107
Acc36I ACCTGC 2 cut(s) 2107, 2274
AccB1I GGYRCC 1 cut(s) 1999
AccB7I CCANNNNNTGG 1 cut(s) 46
AccI GTMKAC 2 cut(s) 1461, 1545
AccII CGCG 1 cut(s) 2302
AcoI YGGCCR 2 cut(s) 639, 1196
AcsI RAATTY 5 cut(s) 472, 865, 903, 1006, 1727
AcuI CTGAAG 7 cut(s) 50, 257, 342, 516, 1612, 1895, 2270
AfaI GTAC 3 cut(s) 1429, 2279, 2305
AfeI AGCGCT 1 cut(s) 149
AfiI CCNNNNNNNGG 8 cut(s) 46, 73, 90, 305, 308, 746, 1205, 1974
AflII CTTAAG 1 cut(s) 1943
AflIII ACRYGT 1 cut(s) 1795
AgsI TTSAA 6 cut(s) 774, 1011, 1314, 1849, 1884, 2240
AhdI GACNNNNNGTC 1 cut(s) 2035
AjiI CACGTC 2 cut(s) 1796, 1940
AjnI CCWGG 5 cut(s) 1087, 1373, 1473, 1592, 2225
AleI CACNNNNGTG 1 cut(s) 936
Alw21I GWGCWC 3 cut(s) 120, 525, 796
Alw26I GTCTC 6 cut(s) 142, 1089, 1647, 1715, 2256, 2266
AlwNI CAGNNNCTG 2 cut(s) 388, 1419
Aor51HI AGCGCT 1 cut(s) 149
AoxI GGCC 9 cut(s) 60, 126, 639, 716, 814, 1196, 1253, 1362, 2228
ApaI GGGCCC 1 cut(s) 130
ApoI RAATTY 5 cut(s) 472, 865, 903, 1006, 1727
Asp700I GAANNNNTTC 2 cut(s) 1132, 1956
AspLEI GCGC 3 cut(s) 150, 350, 2302
AspS9I GGNCC 7 cut(s) 126, 127, 353, 717, 1377, 2143, 2173
AsuC2I CCSGG 1 cut(s) 1975
AsuHPI GGTGA 8 cut(s) 950, 1124, 1342, 1692, 1934, 2071, 2131, 2327
AsuNHI GCTAGC 1 cut(s) 1019
AvaII GGWCC 4 cut(s) 353, 1377, 2143, 2173
BaeGI GKGCMC 3 cut(s) 130, 171, 1985
BalI TGGCCA 1 cut(s) 1198
BamHI GGATCC 1 cut(s) 1370
BanI GGYRCC 1 cut(s) 1999
BanII GRGCYC 3 cut(s) 130, 525, 796
BauI CACGAG 3 cut(s) 795, 1737, 1821
BbsI GAAGAC 2 cut(s) 237, 1872
Bbv12I GWGCWC 3 cut(s) 120, 525, 796
BbvCI CCTCAGC 2 cut(s) 825, 1704
BccI CCATC 4 cut(s) 576, 668, 1168, 1990
BceAI ACGGC 2 cut(s) 626, 654
BciT130I CCWGG 5 cut(s) 1089, 1375, 1475, 1594, 2227
BclI TGATCA 2 cut(s) 961, 988
BcnI CCSGG 1 cut(s) 1975
BcoDI GTCTC 6 cut(s) 142, 1089, 1647, 1715, 2256, 2266
BfaI CTAG 6 cut(s) 789, 1020, 1029, 1431, 1781, 1892
BfmI CTRYAG 4 cut(s) 549, 778, 2130, 2267
BfoI RGCGCY 2 cut(s) 151, 351
BfrI CTTAAG 1 cut(s) 1943
BfuAI ACCTGC 2 cut(s) 2107, 2274
BglII AGATCT 1 cut(s) 1870
BmcAI AGTACT 1 cut(s) 1429
Bme1390I CCNGG 6 cut(s) 1089, 1375, 1475, 1594, 1975, 2227
Bme18I GGWCC 4 cut(s) 353, 1377, 2143, 2173
BmeRI GACNNNNNGTC 1 cut(s) 2035
BmgBI CACGTC 2 cut(s) 1796, 1940
BmgT120I GGNCC 7 cut(s) 126, 127, 353, 717, 1377, 2143, 2173
BmiI GGNNCC 5 cut(s) 128, 1372, 1540, 2001, 2175
BmrFI CCNGG 6 cut(s) 1089, 1375, 1475, 1594, 1975, 2227
BmrI ACTGGG 1 cut(s) 606
BmsI GCATC 6 cut(s) 211, 860, 881, 991, 1725, 2206
BmtI GCTAGC 1 cut(s) 1023
BmuI ACTGGG 1 cut(s) 606
BpiI GAAGAC 2 cut(s) 237, 1872
BpmI CTGGAG 5 cut(s) 327, 1071, 1576, 1807, 2270
Bpu10I CCTNAGC 3 cut(s) 89, 825, 1704
BpuEI CTTGAG 3 cut(s) 53, 136, 522
BpuMI CCSGG 1 cut(s) 1975
BsaAI YACGTR 1 cut(s) 2015
BsaI GGTCTC 1 cut(s) 2256
BsaJI CCNNGG 6 cut(s) 99, 1199, 1256, 1549, 2093, 2310
BsaXI ACNNNNNCTCC 6 cut(s) 640, 670, 1503, 1533, 1780, 1810
Bsc4I CCNNNNNNNGG 8 cut(s) 46, 73, 90, 305, 308, 746, 1205, 1974
Bse118I RCCGGY 2 cut(s) 13, 783
Bse1I ACTGG 5 cut(s) 310, 458, 601, 1790, 1910
Bse3DI GCAATG 1 cut(s) 846
BseBI CCWGG 5 cut(s) 1089, 1375, 1475, 1594, 2227
BseDI CCNNGG 6 cut(s) 99, 1199, 1256, 1549, 2093, 2310
BseGI GGATG 9 cut(s) 109, 851, 872, 1006, 1179, 1462, 1663, 2001, 2173
BseLI CCNNNNNNNGG 8 cut(s) 46, 73, 90, 305, 308, 746, 1205, 1974
BseMI GCAATG 1 cut(s) 846
BseMII CTCAG 6 cut(s) 103, 839, 1718, 2040, 2073, 2272
BseNI ACTGG 5 cut(s) 310, 458, 601, 1790, 1910
BseRI GAGGAG 3 cut(s) 35, 1047, 1372
BseSI GKGCMC 3 cut(s) 130, 171, 1985
BseX3I CGGCCG 1 cut(s) 639
BseYI CCCAGC 2 cut(s) 185, 623
Bsh1236I CGCG 1 cut(s) 2302
Bsh1285I CGRYCG 1 cut(s) 642
BshFI GGCC 9 cut(s) 62, 128, 641, 718, 816, 1198, 1255, 1364, 2230
BshNI GGYRCC 1 cut(s) 1999
BsiEI CGRYCG 1 cut(s) 642
BsiHKAI GWGCWC 3 cut(s) 120, 525, 796
BsiSI CCGG 5 cut(s) 14, 351, 715, 784, 1974
BslFI GGGAC 1 cut(s) 498
BslI CCNNNNNNNGG 8 cut(s) 46, 73, 90, 305, 308, 746, 1205, 1974
BsmAI GTCTC 6 cut(s) 142, 1089, 1647, 1715, 2256, 2266
BsmBI CGTCTC 2 cut(s) 142, 1715
BsmFI GGGAC 1 cut(s) 498
BsmI GAATGC 1 cut(s) 259
BsnI GGCC 9 cut(s) 62, 128, 641, 718, 816, 1198, 1255, 1364, 2230
Bso31I GGTCTC 1 cut(s) 2256
Bsp120I GGGCCC 1 cut(s) 126
Bsp1286I GDGCHC 6 cut(s) 120, 130, 171, 525, 796, 1985
Bsp19I CCATGG 2 cut(s) 1199, 1549
BspANI GGCC 9 cut(s) 62, 128, 641, 718, 816, 1198, 1255, 1364, 2230
BspCNI CTCAG 6 cut(s) 102, 838, 1717, 2039, 2074, 2271
BspFNI CGCG 1 cut(s) 2302
BspHI TCATGA 1 cut(s) 1647
BspLI GGNNCC 5 cut(s) 128, 1372, 1540, 2001, 2175
BspMAI CTGCAG 1 cut(s) 2271
BspMI ACCTGC 2 cut(s) 2107, 2274
BspOI GCTAGC 1 cut(s) 1023
BspT107I GGYRCC 1 cut(s) 1999
BspTI CTTAAG 1 cut(s) 1943
BspTNI GGTCTC 1 cut(s) 2256
BsrDI GCAATG 1 cut(s) 846
BsrFI RCCGGY 2 cut(s) 13, 783
BsrI ACTGG 5 cut(s) 310, 458, 601, 1790, 1910
BssAI RCCGGY 2 cut(s) 13, 783
BssECI CCNNGG 6 cut(s) 99, 1199, 1256, 1549, 2093, 2310
BssNAI GTATAC 1 cut(s) 1546
BssSI CACGAG 3 cut(s) 795, 1737, 1821
BssT1I CCWWGG 4 cut(s) 1199, 1256, 1549, 2093
Bst1107I GTATAC 1 cut(s) 1546
Bst2BI CACGAG 3 cut(s) 795, 1737, 1821
Bst2UI CCWGG 5 cut(s) 1089, 1375, 1475, 1594, 2227
Bst4CI ACNGT 3 cut(s) 1543, 1912, 2277
Bst6I CTCTTC 1 cut(s) 230
BstAFI CTTAAG 1 cut(s) 1943
BstAPI GCANNNNNTGC 1 cut(s) 418
BstBAI YACGTR 1 cut(s) 2015
BstC8I GCNNGC 6 cut(s) 15, 1021, 1414, 1985, 2076, 2207
BstDEI CTNAG 8 cut(s) 89, 526, 825, 1233, 1704, 2026, 2082, 2258
BstDSI CCRYGG 3 cut(s) 1199, 1549, 2310
BstENI CCTNNNNNAGG 1 cut(s) 71
BstF5I GGATG 9 cut(s) 109, 851, 872, 1006, 1179, 1462, 1663, 2001, 2173
BstFNI CGCG 1 cut(s) 2302
BstH2I RGCGCY 2 cut(s) 151, 351
BstHHI GCGC 3 cut(s) 150, 350, 2302
BstMAI GTCTC 6 cut(s) 142, 1089, 1647, 1715, 2256, 2266
BstMCI CGRYCG 1 cut(s) 642
BstNI CCWGG 5 cut(s) 1089, 1375, 1475, 1594, 2227
BstSCI CCNGG 6 cut(s) 1087, 1373, 1473, 1592, 1973, 2225
BstSFI CTRYAG 4 cut(s) 549, 778, 2130, 2267
BstSLI GKGCMC 3 cut(s) 130, 171, 1985
BstSNI TACGTA 1 cut(s) 2015
BstUI CGCG 1 cut(s) 2302
BstV2I GAAGAC 2 cut(s) 237, 1872
BstX2I RGATCY 6 cut(s) 36, 532, 1370, 1870, 2088, 2157
BstYI RGATCY 6 cut(s) 36, 532, 1370, 1870, 2088, 2157
BstZ17I GTATAC 1 cut(s) 1546
BstZI CGGCCG 1 cut(s) 639
BsuRI GGCC 9 cut(s) 62, 128, 641, 718, 816, 1198, 1255, 1364, 2230
BtgI CCRYGG 3 cut(s) 1199, 1549, 2310
BtrI CACGTC 2 cut(s) 1796, 1940
BtsCI GGATG 9 cut(s) 109, 851, 872, 1006, 1179, 1462, 1663, 2001, 2173
BtsI GCAGTG 4 cut(s) 319, 337, 1414, 1444
BtsIMutI CAGTG 6 cut(s) 303, 319, 337, 1414, 1444, 1464
BveI ACCTGC 2 cut(s) 2107, 2274
Cac8I GCNNGC 6 cut(s) 15, 1021, 1414, 1985, 2076, 2207
CaiI CAGNNNCTG 2 cut(s) 388, 1419
CciI TCATGA 1 cut(s) 1647
CfoI GCGC 3 cut(s) 150, 350, 2302
Cfr10I RCCGGY 2 cut(s) 13, 783
Cfr13I GGNCC 7 cut(s) 126, 127, 353, 717, 1377, 2143, 2173
CseI GACGC 1 cut(s) 1697
Csp6I GTAC 3 cut(s) 1428, 2278, 2304
CspCI CAANNNNNGTGG 2 cut(s) 2151, 2186
CviQI GTAC 3 cut(s) 1428, 2278, 2304
DdeI CTNAG 8 cut(s) 89, 526, 825, 1233, 1704, 2026, 2082, 2258
DriI GACNNNNNGTC 1 cut(s) 2035
EaeI YGGCCR 2 cut(s) 639, 1196
EagI CGGCCG 1 cut(s) 639
Eam1104I CTCTTC 1 cut(s) 230
Eam1105I GACNNNNNGTC 1 cut(s) 2035
EarI CTCTTC 1 cut(s) 230
EciI GGCGGA 1 cut(s) 32
Ecl136II GAGCTC 2 cut(s) 523, 794
EclXI CGGCCG 1 cut(s) 639
Eco105I TACGTA 1 cut(s) 2015
Eco130I CCWWGG 4 cut(s) 1199, 1256, 1549, 2093
Eco24I GRGCYC 3 cut(s) 130, 525, 796
Eco31I GGTCTC 1 cut(s) 2256
Eco32I GATATC 3 cut(s) 433, 712, 1152
Eco47I GGWCC 4 cut(s) 353, 1377, 2143, 2173
Eco47III AGCGCT 1 cut(s) 149
Eco52I CGGCCG 1 cut(s) 639
Eco53kI GAGCTC 2 cut(s) 523, 794
Eco57I CTGAAG 7 cut(s) 50, 257, 342, 516, 1612, 1895, 2270
EcoICRI GAGCTC 2 cut(s) 523, 794
EcoNI CCTNNNNNAGG 1 cut(s) 71
EcoO109I RGGNCCY 1 cut(s) 126
EcoRI GAATTC 2 cut(s) 1006, 1727
EcoRII CCWGG 5 cut(s) 1087, 1373, 1473, 1592, 2225
EcoRV GATATC 3 cut(s) 433, 712, 1152
EcoT14I CCWWGG 4 cut(s) 1199, 1256, 1549, 2093
EcoT38I GRGCYC 3 cut(s) 130, 525, 796
ErhI CCWWGG 4 cut(s) 1199, 1256, 1549, 2093
Esp3I CGTCTC 2 cut(s) 142, 1715
FaqI GGGAC 1 cut(s) 498
FauI CCCGC 1 cut(s) 46
FbaI TGATCA 2 cut(s) 961, 988
FblI GTMKAC 2 cut(s) 1461, 1545
FokI GGATG 9 cut(s) 116, 838, 859, 1013, 1186, 1469, 1650, 2008, 2180
FriOI GRGCYC 3 cut(s) 130, 525, 796
FspBI CTAG 6 cut(s) 789, 1020, 1029, 1431, 1781, 1892
GlaI GCGC 3 cut(s) 149, 349, 2301
GsaI CCCAGC 2 cut(s) 189, 627
GsuI CTGGAG 5 cut(s) 327, 1071, 1576, 1807, 2270
HaeII RGCGCY 2 cut(s) 151, 351
HaeIII GGCC 9 cut(s) 62, 128, 641, 718, 816, 1198, 1255, 1364, 2230
HapII CCGG 5 cut(s) 14, 351, 715, 784, 1974
HgaI GACGC 1 cut(s) 1697
HhaI GCGC 3 cut(s) 150, 350, 2302
Hin6I GCGC 3 cut(s) 148, 348, 2300
HinP1I GCGC 3 cut(s) 148, 348, 2300
HincII GTYRAC 1 cut(s) 1396
HindII GTYRAC 1 cut(s) 1396
HinfI GANTC 7 cut(s) 180, 736, 764, 770, 1106, 1166, 1283
HpaI GTTAAC 1 cut(s) 1396
HpaII CCGG 5 cut(s) 14, 351, 715, 784, 1974
HphI GGTGA 8 cut(s) 950, 1124, 1342, 1692, 1934, 2071, 2131, 2327
Hpy166II GTNNAC 6 cut(s) 367, 636, 1396, 1462, 1546, 2173
Hpy8I GTNNAC 6 cut(s) 367, 636, 1396, 1462, 1546, 2173
Hpy99I CGWCG 1 cut(s) 1797
HpyAV CCTTC 9 cut(s) 67, 168, 366, 929, 1118, 1143, 1214, 1636, 1972
HpyCH4III ACNGT 3 cut(s) 1543, 1912, 2277
HpyCH4IV ACGT 3 cut(s) 1795, 1939, 2014
HpyCH4V TGCA 8 cut(s) 400, 421, 851, 1264, 1716, 2116, 2219, 2269
HpyF3I CTNAG 8 cut(s) 89, 526, 825, 1233, 1704, 2026, 2082, 2258
HpySE526I ACGT 3 cut(s) 1795, 1939, 2014
HspAI GCGC 3 cut(s) 148, 348, 2300
KroI GCCGGC 1 cut(s) 13
KroNI GCCGGC 1 cut(s) 15
Ksp22I TGATCA 2 cut(s) 961, 988
KspAI GTTAAC 1 cut(s) 1396
LmnI GCTCC 4 cut(s) 22, 1688, 2071, 2126
LweI GCATC 6 cut(s) 211, 860, 881, 991, 1725, 2206
MaeI CTAG 6 cut(s) 789, 1020, 1029, 1431, 1781, 1892
MaeII ACGT 3 cut(s) 1795, 1939, 2014
MaeIII GTNAC 5 cut(s) 215, 646, 799, 1693, 1720
MfeI CAATTG 1 cut(s) 405
MflI RGATCY 6 cut(s) 36, 532, 1370, 1870, 2088, 2157
MhlI GDGCHC 6 cut(s) 120, 130, 171, 525, 796, 1985
MlsI TGGCCA 1 cut(s) 1198
MluNI TGGCCA 1 cut(s) 1198
MlyI GAGTC 1 cut(s) 189
Mox20I TGGCCA 1 cut(s) 1198
MreI CGCCGGCG 1 cut(s) 13
MroNI GCCGGC 1 cut(s) 13
MroXI GAANNNNTTC 2 cut(s) 1132, 1956
MscI TGGCCA 1 cut(s) 1198
MseI TTAA 4 cut(s) 732, 1389, 1395, 1944
MslI CAYNNNNRTG 4 cut(s) 936, 1198, 1278, 1668
Msp20I TGGCCA 1 cut(s) 1198
MspA1I CMGCKG 3 cut(s) 362, 1419, 2222
MspCI CTTAAG 1 cut(s) 1943
MspI CCGG 5 cut(s) 14, 351, 715, 784, 1974
MspR9I CCNGG 6 cut(s) 1089, 1375, 1475, 1594, 1975, 2227
MunI CAATTG 1 cut(s) 405
Mva1269I GAATGC 1 cut(s) 259
MvaI CCWGG 5 cut(s) 1089, 1375, 1475, 1594, 2227
MvnI CGCG 1 cut(s) 2302
NaeI GCCGGC 1 cut(s) 15
NciI CCSGG 1 cut(s) 1975
NcoI CCATGG 2 cut(s) 1199, 1549
NgoMIV GCCGGC 1 cut(s) 13
NheI GCTAGC 1 cut(s) 1019
NlaIV GGNNCC 5 cut(s) 128, 1372, 1540, 2001, 2175
NmuCI GTSAC 2 cut(s) 215, 1693
OliI CACNNNNGTG 1 cut(s) 936
PagI TCATGA 1 cut(s) 1647
PaqCI CACCTGC 1 cut(s) 2107
PctI GAATGC 1 cut(s) 259
PdiI GCCGGC 1 cut(s) 15
PdmI GAANNNNTTC 2 cut(s) 1132, 1956
PfeI GAWTC 6 cut(s) 736, 764, 770, 1106, 1166, 1283
PflFI GACNNNGTC 1 cut(s) 510
PflMI CCANNNNNTGG 1 cut(s) 46
PfoI TCCNGGA 2 cut(s) 1087, 1373
PleI GAGTC 1 cut(s) 188
PpsI GAGTC 1 cut(s) 188
Ppu21I YACGTR 1 cut(s) 2015
PsiI TTATAA 1 cut(s) 446
Psp124BI GAGCTC 2 cut(s) 525, 796
Psp6I CCWGG 5 cut(s) 1087, 1373, 1473, 1592, 2225
PspFI CCCAGC 2 cut(s) 185, 623
PspGI CCWGG 5 cut(s) 1087, 1373, 1473, 1592, 2225
PspN4I GGNNCC 5 cut(s) 128, 1372, 1540, 2001, 2175
PspOMI GGGCCC 1 cut(s) 126
PspPI GGNCC 7 cut(s) 126, 127, 353, 717, 1377, 2143, 2173
PstI CTGCAG 1 cut(s) 2271
PstNI CAGNNNCTG 2 cut(s) 388, 1419
PsuI RGATCY 6 cut(s) 36, 532, 1370, 1870, 2088, 2157
PsyI GACNNNGTC 1 cut(s) 510
PvuII CAGCTG 3 cut(s) 362, 1419, 2222
RsaI GTAC 3 cut(s) 1429, 2279, 2305
RsaNI GTAC 3 cut(s) 1428, 2278, 2304
RseI CAYNNNNRTG 4 cut(s) 936, 1198, 1278, 1668
SacI GAGCTC 2 cut(s) 525, 796
SaqAI TTAA 4 cut(s) 732, 1389, 1395, 1944
Sau96I GGNCC 7 cut(s) 126, 127, 353, 717, 1377, 2143, 2173
ScaI AGTACT 1 cut(s) 1429
SchI GAGTC 1 cut(s) 189
ScrFI CCNGG 6 cut(s) 1089, 1375, 1475, 1594, 1975, 2227
SduI GDGCHC 6 cut(s) 120, 130, 171, 525, 796, 1985
SfaNI GCATC 6 cut(s) 211, 860, 881, 991, 1725, 2206
SfcI CTRYAG 4 cut(s) 549, 778, 2130, 2267
SgrAI CRCCGGYG 1 cut(s) 13
SinI GGWCC 4 cut(s) 353, 1377, 2143, 2173
SmiMI CAYNNNNRTG 4 cut(s) 936, 1198, 1278, 1668
SmlI CTYRAG 4 cut(s) 68, 151, 537, 1943
SmoI CTYRAG 4 cut(s) 68, 151, 537, 1943
SnaBI TACGTA 1 cut(s) 2015
SspI AATATT 2 cut(s) 1072, 1604
SspMI CTAG 6 cut(s) 789, 1020, 1029, 1431, 1781, 1892
SstI GAGCTC 2 cut(s) 525, 796
StyD4I CCNGG 6 cut(s) 1087, 1373, 1473, 1592, 1973, 2225
StyI CCWWGG 4 cut(s) 1199, 1256, 1549, 2093
TaaI ACNGT 3 cut(s) 1543, 1912, 2277
TaiI ACGT 3 cut(s) 1798, 1942, 2017
TaqI TCGA 4 cut(s) 724, 1136, 1660, 1867
TatI WGTACW 2 cut(s) 1427, 2277
TfiI GAWTC 6 cut(s) 736, 764, 770, 1106, 1166, 1283
Tru1I TTAA 4 cut(s) 732, 1389, 1395, 1944
Tru9I TTAA 4 cut(s) 732, 1389, 1395, 1944
TscAI CASTG 6 cut(s) 310, 319, 337, 1414, 1444, 1471
TseFI GTSAC 2 cut(s) 215, 1693
Tsp45I GTSAC 2 cut(s) 215, 1693
TspDTI ATGAA 9 cut(s) 240, 1284, 1350, 1664, 1829, 1844, 1853, 2034, 2128
TspGWI ACGGA 1 cut(s) 124
TspRI CASTG 6 cut(s) 310, 319, 337, 1414, 1444, 1471
Tth111I GACNNNGTC 1 cut(s) 510
Van91I CCANNNNNTGG 1 cut(s) 46
Vha464I CTTAAG 1 cut(s) 1943
VpaK11BI GGWCC 4 cut(s) 353, 1377, 2143, 2173
XagI CCTNNNNNAGG 1 cut(s) 71
XapI RAATTY 5 cut(s) 472, 865, 903, 1006, 1727
XbaI TCTAGA 1 cut(s) 788
XmiI GTMKAC 2 cut(s) 1461, 1545
XmnI GAANNNNTTC 2 cut(s) 1132, 1956
XspI CTAG 6 cut(s) 789, 1020, 1029, 1431, 1781, 1892
ZrmI AGTACT 1 cut(s) 1429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.