Rh4AG259200

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000660
Physical Location & Seq
Forward (+)
57509725 .. 57516094
6370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG259200.1

Sequence Viewer

Length: 2112 bp
ATGGTCGAAAGTGATCTCGACCACCTCAAGCAAGAAATGTCCCAGCTCACCAGCCACCGCCGAATCGCCAGCTCCGGTACTCCGCAGCCCAAAACGAGCGGCAGTGCCAGTCGGCTGCCGTCGACTTTTAACTTGAGTGATGCTCAGTATCTCAACATATTGCAGTCGATTGGGCAGTCTGTGCATGCAATGGATCGTAGTGGTCGTATAATCTACTGGAACAGAAGTGCTGAAAAACTGTATGGTTATTCTCCGGAAGAGGCCCTTGGACAGGTTTCCATTAAGCTCATAGTAGATTCCCGGGACTTTGGTGTAGCATATAATATAGTCCACCGTGTCATCCACGGGGAGAGTTGGACTGGGCAGTTCCCTGTAAAGCACAAATCGGGGGCGAGGTTTTCAGTCATTACAACAAATACTCCATTCTATGATGATGCTGGCAGTTTAATTGGGGTTGTTGCTACATCAACTGATTCTCAGCCCTTTTATGAAATGAGGGTTCCAATGTCAGCTGAGAAGCAACCACAACAAGATCTAAGCTTCAGCAGTCGTGGGGCTAGTGTTACAACTAAATTCGGTGCTGATCCTCAGCAGCCTCTGCGAAAAGCGGTTTCGTCAAAACTAACAGACTTGGCATCGAAGATGAGTTTCAAAGTCAAGTCAAAAATTCAGGCAAGAGAAAACAACATGGATCCCAAAGGTGACAGCGAAGATAGTCATCATCCGTTTCATGGTTCAGATTCTGTCACATCTGCCAATAGGGAGGATGGGAATGCAAGTGGGACTAGCACAGCTACAGGAACTATACCCCCAGCAACTCCTTTTGGTGTATTTAACCATGTTGAGAGCAAGCCTCTAGGAGAACCCTCCATAGACTCTGCCAGTGAGAGCAAAGAAAAATCTTCAATTCACAAGCTAATTGCCTCAAAGGCAGAAGCATGGATTAGTAAGCAGGAGATATCATGGCCTTGGAAAGGAAATGAGCGAGATGGGTCAGTGGCTAGGGCCAGTGGTTTTGTTTGGCCCTGGTTTCATAATGATCAAGTCAATTTTGCCATTCCTCAGAAGAGTTATTGTGTCTCAGAACCAGAAATTCAGGTGAGCGAAAGTAATCGGCCAACAAATAATAATGATGAGACTCAAGGATCCTGGACCTCTGCATTGGATATTAACAGCATAAGCCGTGCCATCAGCCTTAGAAGGACCAGTAGTGCTTTGCCTACGGTGGATGTGGACACTGATTGCTTGGATTATGAAATCTTATGGGAAGACATGATAATTGGAAGACAGATTGGGCAAGGTTCATGTGGAACTGTATACCATGGTCAGTGGGATGGATCAGATGTTGCCGTTAAGGTATTCTCCAAGTTAGAATATCCGGATGATTTGTTACTTTCCTTCAAACAAGAGGTATCTCTAATGAAAAGGCTTCGACATCCAAATGTTTTGCTTTTCATGGGAGCGGTGACTTCACCTGAGCGTCTCTGCATTGTCACAGAGTTCCTCCCACGTGGAAGTTTGTATCAAATACTACAGAGGAACCCATCAAAACTAGACTGGAGACGACGTATTAATATGGCTATGGATGTAGCATGGGGCATGAATTATCTTCACCATTTCAACCCACCAATCATTCATCGAGACTTAAAGTCTTCAAATCTCCTAGTTGATAAGAACTGGACTGTCAAGGTTGGCGATTTTGGTCTTTCACGTTTTAAGCATGAAACTTTTCTTATAAACAAGAGTGGCAGGGGAACGCCTCAATGGATGTCACCAGAATTTCTACGTAGTGAACCCTCGGATGAGAAGTCTGACATATATAGCTACGGGGTGATATTGTGGGAGCTTGCTACTGAGAAGATTCCTTGGGATAATCTAAACCCAATGCAGGTGATTGGAGCTGTTGGATTCATGAACCAAAGACTGGAGATCCCAAAAGATGTGGACCCAAAGTGGGCTTCTATAATTGAGAGTTGCTGGCACAGTGATCCAGCTAGCCGGCCAACATTTCTAGAACTGCTCGAAAAGCTTAGAGTGTTGCATAGACAATATAATCTCCAGTTCAAAGCAGCGCGTTCCGCCTCTAGTGAGAGCACCCAAAAGGAATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS PAS_9 Pkinase_Tyr

Protein Analysis

703

Amino Acids

78.82

Weight (kDa)

6.59

Isoelectric Point (pI)

47.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS PF00989 49 - 155 3.2e-11 PAS fold
PAS_4 PF08448 55 - 159 3.6e-09 PAS fold
PAS_9 PF13426 65 - 158 4.6e-08 PAS domain
PAS_3 PF08447 70 - 155 1.6e-08 PAS fold
PK_Tyr_Ser-Thr PF07714 427 - 677 1.2e-65 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 428 - 675 7.2e-52 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1736
AarI CACCTGC 1 cut(s) 1879
Acc36I ACCTGC 1 cut(s) 1879
AccB7I CCANNNNNTGG 1 cut(s) 1924
AccBSI CCGCTC 2 cut(s) 99, 1463
AccI GTMKAC 2 cut(s) 122, 1317
AccII CGCG 1 cut(s) 2074
AccIII TCCGGA 2 cut(s) 253, 1378
AciI CCGC 6 cut(s) 58, 83, 99, 608, 1463, 2079
AclWI GGATC 9 cut(s) 201, 578, 686, 699, 1140, 1153, 1345, 1924, 1982
AcoI YGGCCR 2 cut(s) 1115, 2000
AcsI RAATTY 4 cut(s) 572, 666, 1092, 1778
AcuI CTGAAG 1 cut(s) 526
AcvI CACGTG 1 cut(s) 1511
AfaI GTAC 1 cut(s) 79
AfiI CCNNNNNNNGG 6 cut(s) 271, 731, 974, 1888, 1924, 1954
AgsI TTSAA 6 cut(s) 652, 906, 1402, 1621, 1656, 2065
AhdI GACNNNNNGTC 1 cut(s) 1648
AjnI CCWGG 2 cut(s) 1025, 1148
AjuI GAANNNNNNNTTGG 4 cut(s) 249, 281, 1942, 1974
Alw21I GWGCWC 1 cut(s) 2096
Alw26I GTCTC 5 cut(s) 1084, 1130, 1487, 1555, 1635
AlwI GGATC 9 cut(s) 201, 578, 686, 699, 1140, 1153, 1345, 1924, 1982
AlwNI CAGNNNCTG 2 cut(s) 598, 743
Ama87I CYCGRG 1 cut(s) 300
Aor13HI TCCGGA 2 cut(s) 253, 1378
AoxI GGCC 6 cut(s) 261, 965, 1005, 1022, 1115, 2000
ApeKI GCWGC 4 cut(s) 85, 115, 592, 2069
ApoI RAATTY 4 cut(s) 572, 666, 1092, 1778
AseI ATTAAT 1 cut(s) 1572
Asp700I GAANNNNTTC 1 cut(s) 1728
AspLEI GCGC 1 cut(s) 2074
AspS9I GGNCC 6 cut(s) 262, 1005, 1023, 1152, 1203, 1945
AsuC2I CCSGG 2 cut(s) 301, 302
AsuHPI GGTGA 9 cut(s) 40, 713, 1111, 1464, 1477, 1604, 1764, 1843, 1903
AsuNHI GCTAGC 1 cut(s) 1994
AvaI CYCGRG 1 cut(s) 300
AvaII GGWCC 3 cut(s) 1152, 1203, 1945
BamHI GGATCC 2 cut(s) 691, 1145
BbrPI CACGTG 1 cut(s) 1511
BbsI GAAGAC 3 cut(s) 1275, 1291, 1644
Bbv12I GWGCWC 1 cut(s) 2096
BbvCI CCTCAGC 1 cut(s) 588
BbvI GCAGC 4 cut(s) 97, 102, 604, 2081
BccI CCATC 5 cut(s) 761, 983, 1196, 1328, 1552
BceAI ACGGC 3 cut(s) 103, 1167, 1334
BciT130I CCWGG 2 cut(s) 1027, 1150
BclI TGATCA 1 cut(s) 1039
BcnI CCSGG 2 cut(s) 301, 302
BcoDI GTCTC 5 cut(s) 1084, 1130, 1487, 1555, 1635
BfaI CTAG 9 cut(s) 558, 786, 857, 1002, 1553, 1664, 1995, 2012, 2085
BfmI CTRYAG 2 cut(s) 795, 1532
BfuAI ACCTGC 1 cut(s) 1879
BglI GCCNNNNNGGC 1 cut(s) 929
BglII AGATCT 1 cut(s) 532
BisI GCNGC 5 cut(s) 86, 100, 116, 593, 2070
BlsI GCNGC 5 cut(s) 87, 101, 117, 594, 2071
Bme1390I CCNGG 4 cut(s) 301, 302, 1027, 1150
Bme18I GGWCC 3 cut(s) 1152, 1203, 1945
BmeRI GACNNNNNGTC 1 cut(s) 1648
BmeT110I CYCGRG 1 cut(s) 300
BmgT120I GGNCC 6 cut(s) 262, 1005, 1023, 1152, 1203, 1945
BmiI GGNNCC 5 cut(s) 501, 693, 1147, 1541, 1947
BmrFI CCNGG 4 cut(s) 301, 302, 1027, 1150
BmrI ACTGGG 1 cut(s) 369
BmsI GCATC 3 cut(s) 130, 424, 644
BmtI GCTAGC 1 cut(s) 1998
BmuI ACTGGG 1 cut(s) 369
BpiI GAAGAC 3 cut(s) 1275, 1291, 1644
BplI GAGNNNNNCTC 4 cut(s) 127, 159, 838, 870
BpmI CTGGAG 3 cut(s) 1579, 1946, 2042
Bpu10I CCTNAGC 2 cut(s) 588, 1476
BpuEI CTTGAG 3 cut(s) 11, 154, 1125
BpuMI CCSGG 2 cut(s) 301, 302
BsaAI YACGTR 2 cut(s) 1511, 1787
BsaBI GATNNNNATC 1 cut(s) 717
BsaJI CCNNGG 8 cut(s) 265, 300, 343, 968, 1025, 1321, 1797, 1865
BsaWI WCCGGW 3 cut(s) 74, 253, 1378
BsaXI ACNNNNNCTCC 4 cut(s) 403, 433, 1552, 1582
Bsc4I CCNNNNNNNGG 6 cut(s) 271, 731, 974, 1888, 1924, 1954
Bse118I RCCGGY 1 cut(s) 1998
Bse3DI GCAATG 1 cut(s) 195
Bse8I GATNNNNATC 1 cut(s) 717
BseAI TCCGGA 2 cut(s) 253, 1378
BseBI CCWGG 2 cut(s) 1027, 1150
BseDI CCNNGG 8 cut(s) 265, 300, 343, 968, 1025, 1321, 1797, 1865
BseJI GATNNNNATC 1 cut(s) 717
BseLI CCNNNNNNNGG 6 cut(s) 271, 731, 974, 1888, 1924, 1954
BseMI GCAATG 1 cut(s) 195
BseMII CTCAG 8 cut(s) 158, 491, 504, 602, 1076, 1095, 1467, 1845
BseXI GCAGC 4 cut(s) 97, 102, 604, 2081
BseYI CCCAGC 2 cut(s) 42, 811
Bsh1236I CGCG 1 cut(s) 2074
BshFI GGCC 6 cut(s) 263, 967, 1007, 1024, 1117, 2002
BsiHKAI GWGCWC 1 cut(s) 2096
BsiHKCI CYCGRG 1 cut(s) 300
BsiSI CCGG 5 cut(s) 75, 254, 301, 1379, 1999
BslFI GGGAC 3 cut(s) 25, 317, 796
BslI CCNNNNNNNGG 6 cut(s) 271, 731, 974, 1888, 1924, 1954
BsmAI GTCTC 5 cut(s) 1084, 1130, 1487, 1555, 1635
BsmBI CGTCTC 2 cut(s) 1487, 1555
BsmFI GGGAC 3 cut(s) 25, 317, 796
BsmI GAATGC 1 cut(s) 778
BsnI GGCC 6 cut(s) 263, 967, 1007, 1024, 1117, 2002
BsoBI CYCGRG 1 cut(s) 300
Bsp1286I GDGCHC 1 cut(s) 2096
Bsp13I TCCGGA 2 cut(s) 253, 1378
Bsp19I CCATGG 1 cut(s) 1321
BspACI CCGC 6 cut(s) 58, 83, 99, 608, 1463, 2079
BspANI GGCC 6 cut(s) 263, 967, 1007, 1024, 1117, 2002
BspCNI CTCAG 8 cut(s) 157, 490, 505, 601, 1075, 1094, 1468, 1846
BspEI TCCGGA 2 cut(s) 253, 1378
BspFNI CGCG 1 cut(s) 2074
BspHI TCATGA 1 cut(s) 1911
BspLI GGNNCC 5 cut(s) 501, 693, 1147, 1541, 1947
BspMI ACCTGC 1 cut(s) 1879
BspOI GCTAGC 1 cut(s) 1998
BspPI GGATC 9 cut(s) 201, 578, 686, 699, 1140, 1153, 1345, 1924, 1982
BsrBI CCGCTC 2 cut(s) 99, 1463
BsrDI GCAATG 1 cut(s) 195
BsrFI RCCGGY 1 cut(s) 1998
BssAI RCCGGY 1 cut(s) 1998
BssECI CCNNGG 8 cut(s) 265, 300, 343, 968, 1025, 1321, 1797, 1865
BssNAI GTATAC 1 cut(s) 1318
BssT1I CCWWGG 4 cut(s) 265, 968, 1321, 1865
Bst1107I GTATAC 1 cut(s) 1318
Bst2UI CCWGG 2 cut(s) 1027, 1150
Bst4CI ACNGT 6 cut(s) 240, 335, 1225, 1315, 1684, 1985
Bst6I CTCTTC 2 cut(s) 252, 1061
BstAPI GCANNNNNTGC 2 cut(s) 181, 598
BstBAI YACGTR 2 cut(s) 1511, 1787
BstC8I GCNNGC 8 cut(s) 70, 186, 439, 851, 1848, 1979, 1996, 2000
BstDSI CCRYGG 2 cut(s) 343, 1321
BstENI CCTNNNNNAGG 2 cut(s) 269, 972
BstFNI CGCG 1 cut(s) 2074
BstHHI GCGC 1 cut(s) 2074
BstMAI GTCTC 5 cut(s) 1084, 1130, 1487, 1555, 1635
BstMWI GCNNNNNNNGC 5 cut(s) 181, 598, 929, 2026, 2078
BstNI CCWGG 2 cut(s) 1027, 1150
BstNSI RCATGY 1 cut(s) 188
BstSCI CCNGG 4 cut(s) 299, 300, 1025, 1148
BstSFI CTRYAG 2 cut(s) 795, 1532
BstSNI TACGTA 1 cut(s) 1787
BstUI CGCG 1 cut(s) 2074
BstV1I GCAGC 4 cut(s) 97, 102, 604, 2081
BstV2I GAAGAC 3 cut(s) 1275, 1291, 1644
BstX2I RGATCY 4 cut(s) 532, 691, 1145, 1929
BstYI RGATCY 4 cut(s) 532, 691, 1145, 1929
BstZ17I GTATAC 1 cut(s) 1318
BsuRI GGCC 6 cut(s) 263, 967, 1007, 1024, 1117, 2002
BtgI CCRYGG 2 cut(s) 343, 1321
BtsI GCAGTG 1 cut(s) 109
BtsIMutI CAGTG 7 cut(s) 109, 889, 1002, 1015, 1236, 1334, 1990
BveI ACCTGC 1 cut(s) 1879
Cac8I GCNNGC 8 cut(s) 70, 186, 439, 851, 1848, 1979, 1996, 2000
CaiI CAGNNNCTG 2 cut(s) 598, 743
CciI TCATGA 1 cut(s) 1911
CfoI GCGC 1 cut(s) 2074
Cfr10I RCCGGY 1 cut(s) 1998
Cfr13I GGNCC 6 cut(s) 262, 1005, 1023, 1152, 1203, 1945
Cfr9I CCCGGG 1 cut(s) 300
CseI GACGC 1 cut(s) 1469
Csp6I GTAC 1 cut(s) 78
CspCI CAANNNNNGTGG 2 cut(s) 1923, 1958
CviQI GTAC 1 cut(s) 78
DriI GACNNNNNGTC 1 cut(s) 1648
EaeI YGGCCR 2 cut(s) 1115, 2000
Eam1104I CTCTTC 2 cut(s) 252, 1061
Eam1105I GACNNNNNGTC 1 cut(s) 1648
EarI CTCTTC 2 cut(s) 252, 1061
EciI GGCGGA 1 cut(s) 2068
Eco105I TACGTA 1 cut(s) 1787
Eco130I CCWWGG 4 cut(s) 265, 968, 1321, 1865
Eco32I GATATC 1 cut(s) 960
Eco47I GGWCC 3 cut(s) 1152, 1203, 1945
Eco57I CTGAAG 1 cut(s) 526
Eco72I CACGTG 1 cut(s) 1511
Eco88I CYCGRG 1 cut(s) 300
EcoNI CCTNNNNNAGG 2 cut(s) 269, 972
EcoO109I RGGNCCY 1 cut(s) 262
EcoRII CCWGG 2 cut(s) 1025, 1148
EcoRV GATATC 1 cut(s) 960
EcoT14I CCWWGG 4 cut(s) 265, 968, 1321, 1865
ErhI CCWWGG 4 cut(s) 265, 968, 1321, 1865
Esp3I CGTCTC 2 cut(s) 1487, 1555
FalI AAGNNNNNCTT 2 cut(s) 249, 281
FaqI GGGAC 3 cut(s) 25, 317, 796
FbaI TGATCA 1 cut(s) 1039
FblI GTMKAC 2 cut(s) 122, 1317
Fnu4HI GCNGC 5 cut(s) 86, 100, 116, 593, 2070
Fsp4HI GCNGC 5 cut(s) 86, 100, 116, 593, 2070
FspBI CTAG 9 cut(s) 558, 786, 857, 1002, 1553, 1664, 1995, 2012, 2085
GlaI GCGC 1 cut(s) 2073
GluI GCNGC 5 cut(s) 86, 100, 116, 593, 2070
GsaI CCCAGC 2 cut(s) 46, 815
GsuI CTGGAG 3 cut(s) 1579, 1946, 2042
HaeIII GGCC 6 cut(s) 263, 967, 1007, 1024, 1117, 2002
HapII CCGG 5 cut(s) 75, 254, 301, 1379, 1999
HgaI GACGC 1 cut(s) 1469
HhaI GCGC 1 cut(s) 2074
Hin6I GCGC 1 cut(s) 2072
HinP1I GCGC 1 cut(s) 2072
HincII GTYRAC 1 cut(s) 123
HindII GTYRAC 1 cut(s) 123
HindIII AAGCTT 2 cut(s) 538, 2027
HinfI GANTC 8 cut(s) 63, 296, 473, 740, 875, 1138, 1861, 1908
HpaII CCGG 5 cut(s) 75, 254, 301, 1379, 1999
HphI GGTGA 9 cut(s) 40, 713, 1111, 1464, 1477, 1604, 1764, 1843, 1903
Hpy166II GTNNAC 6 cut(s) 123, 331, 1234, 1318, 1793, 1945
Hpy188I TCNGA 6 cut(s) 739, 1065, 1084, 1342, 1801, 1813
Hpy188III TCNNGA 6 cut(s) 17, 254, 1379, 1640, 1912, 2012
Hpy8I GTNNAC 6 cut(s) 123, 331, 1234, 1318, 1793, 1945
Hpy99I CGWCG 2 cut(s) 124, 1569
HpyAV CCTTC 2 cut(s) 1194, 1408
HpyCH4III ACNGT 6 cut(s) 240, 335, 1225, 1315, 1684, 1985
HpyCH4IV ACGT 4 cut(s) 1510, 1567, 1711, 1786
HpyCH4V TGCA 8 cut(s) 163, 184, 188, 776, 1160, 1488, 1888, 2041
HpyF10VI GCNNNNNNNGC 5 cut(s) 181, 598, 929, 2026, 2078
HpySE526I ACGT 4 cut(s) 1510, 1567, 1711, 1786
HspAI GCGC 1 cut(s) 2072
Kpn2I TCCGGA 2 cut(s) 253, 1378
KroI GCCGGC 1 cut(s) 1998
KroNI GCCGGC 1 cut(s) 2000
Ksp22I TGATCA 1 cut(s) 1039
LmnI GCTCC 4 cut(s) 77, 1460, 1843, 1898
Lsp1109I GCAGC 4 cut(s) 97, 102, 604, 2081
LweI GCATC 3 cut(s) 130, 424, 644
MaeI CTAG 9 cut(s) 558, 786, 857, 1002, 1553, 1664, 1995, 2012, 2085
MaeII ACGT 4 cut(s) 1510, 1567, 1711, 1786
MaeIII GTNAC 7 cut(s) 562, 701, 745, 1389, 1465, 1492, 1770
MbiI CCGCTC 2 cut(s) 99, 1463
MflI RGATCY 4 cut(s) 532, 691, 1145, 1929
MhlI GDGCHC 1 cut(s) 2096
MlyI GAGTC 2 cut(s) 869, 1132
MmeI TCCRAC 2 cut(s) 335, 1885
MroI TCCGGA 2 cut(s) 253, 1378
MroNI GCCGGC 1 cut(s) 1998
MroXI GAANNNNTTC 1 cut(s) 1728
MseI TTAA 9 cut(s) 129, 282, 446, 834, 1170, 1353, 1572, 1646, 1716
MslI CAYNNNNRTG 1 cut(s) 1440
MspA1I CMGCKG 1 cut(s) 512
MspI CCGG 5 cut(s) 75, 254, 301, 1379, 1999
MspR9I CCNGG 4 cut(s) 301, 302, 1027, 1150
Mva1269I GAATGC 1 cut(s) 778
MvaI CCWGG 2 cut(s) 1027, 1150
MvnI CGCG 1 cut(s) 2074
MwoI GCNNNNNNNGC 5 cut(s) 181, 598, 929, 2026, 2078
NaeI GCCGGC 1 cut(s) 2000
NciI CCSGG 2 cut(s) 301, 302
NcoI CCATGG 1 cut(s) 1321
NgoMIV GCCGGC 1 cut(s) 1998
NheI GCTAGC 1 cut(s) 1994
NlaIV GGNNCC 5 cut(s) 501, 693, 1147, 1541, 1947
NmuCI GTSAC 5 cut(s) 701, 745, 1465, 1492, 1770
NspI RCATGY 1 cut(s) 188
PaeI GCATGC 1 cut(s) 188
PagI TCATGA 1 cut(s) 1911
PaqCI CACCTGC 1 cut(s) 1879
PcsI WCGNNNNNNNCGW 1 cut(s) 202
PctI GAATGC 1 cut(s) 778
PdiI GCCGGC 1 cut(s) 2000
PdmI GAANNNNTTC 1 cut(s) 1728
PfeI GAWTC 6 cut(s) 63, 296, 473, 740, 1861, 1908
PflMI CCANNNNNTGG 1 cut(s) 1924
PfoI TCCNGGA 1 cut(s) 1148
PkrI GCNGC 5 cut(s) 87, 101, 117, 594, 2071
PleI GAGTC 2 cut(s) 869, 1132
PmaCI CACGTG 1 cut(s) 1511
PmlI CACGTG 1 cut(s) 1511
PpsI GAGTC 2 cut(s) 869, 1132
Ppu21I YACGTR 2 cut(s) 1511, 1787
PshBI ATTAAT 1 cut(s) 1572
PsiI TTATAA 1 cut(s) 1736
Psp6I CCWGG 2 cut(s) 1025, 1148
PspCI CACGTG 1 cut(s) 1511
PspFI CCCAGC 2 cut(s) 42, 811
PspGI CCWGG 2 cut(s) 1025, 1148
PspN4I GGNNCC 5 cut(s) 501, 693, 1147, 1541, 1947
PspPI GGNCC 6 cut(s) 262, 1005, 1023, 1152, 1203, 1945
PstNI CAGNNNCTG 2 cut(s) 598, 743
PsuI RGATCY 4 cut(s) 532, 691, 1145, 1929
PvuII CAGCTG 1 cut(s) 512
RsaI GTAC 1 cut(s) 79
RsaNI GTAC 1 cut(s) 78
RseI CAYNNNNRTG 1 cut(s) 1440
SalI GTCGAC 1 cut(s) 121
SaqAI TTAA 9 cut(s) 129, 282, 446, 834, 1170, 1353, 1572, 1646, 1716
SatI GCNGC 5 cut(s) 86, 100, 116, 593, 2070
Sau96I GGNCC 6 cut(s) 262, 1005, 1023, 1152, 1203, 1945
SchI GAGTC 2 cut(s) 869, 1132
ScrFI CCNGG 4 cut(s) 301, 302, 1027, 1150
SduI GDGCHC 1 cut(s) 2096
SfaNI GCATC 3 cut(s) 130, 424, 644
SfcI CTRYAG 2 cut(s) 795, 1532
SinI GGWCC 3 cut(s) 1152, 1203, 1945
SmaI CCCGGG 1 cut(s) 302
SmiMI CAYNNNNRTG 1 cut(s) 1440
SmlI CTYRAG 3 cut(s) 26, 133, 1140
SmoI CTYRAG 3 cut(s) 26, 133, 1140
SnaBI TACGTA 1 cut(s) 1787
SphI GCATGC 1 cut(s) 188
SsiI CCGC 6 cut(s) 58, 83, 99, 608, 1463, 2079
SspMI CTAG 9 cut(s) 558, 786, 857, 1002, 1553, 1664, 1995, 2012, 2085
StyD4I CCNGG 4 cut(s) 299, 300, 1025, 1148
StyI CCWWGG 4 cut(s) 265, 968, 1321, 1865
TaaI ACNGT 6 cut(s) 240, 335, 1225, 1315, 1684, 1985
TaiI ACGT 4 cut(s) 1513, 1570, 1714, 1789
TaqI TCGA 8 cut(s) 6, 18, 122, 167, 638, 1432, 1639, 2022
TauI GCSGC 1 cut(s) 102
TfiI GAWTC 6 cut(s) 63, 296, 473, 740, 1861, 1908
Tru1I TTAA 9 cut(s) 129, 282, 446, 834, 1170, 1353, 1572, 1646, 1716
Tru9I TTAA 9 cut(s) 129, 282, 446, 834, 1170, 1353, 1572, 1646, 1716
TscAI CASTG 7 cut(s) 109, 889, 1002, 1015, 1243, 1334, 1990
TseFI GTSAC 5 cut(s) 701, 745, 1465, 1492, 1770
TseI GCWGC 4 cut(s) 85, 115, 592, 2069
Tsp45I GTSAC 5 cut(s) 701, 745, 1465, 1492, 1770
TspGWI ACGGA 1 cut(s) 714
TspMI CCCGGG 1 cut(s) 300
TspRI CASTG 7 cut(s) 109, 889, 1002, 1015, 1243, 1334, 1990
Van91I CCANNNNNTGG 1 cut(s) 1924
VpaK11BI GGWCC 3 cut(s) 1152, 1203, 1945
VspI ATTAAT 1 cut(s) 1572
XagI CCTNNNNNAGG 2 cut(s) 269, 972
XapI RAATTY 4 cut(s) 572, 666, 1092, 1778
XbaI TCTAGA 1 cut(s) 2011
XceI RCATGY 1 cut(s) 188
XmaI CCCGGG 1 cut(s) 300
XmiI GTMKAC 2 cut(s) 122, 1317
XmnI GAANNNNTTC 1 cut(s) 1728
XspI CTAG 9 cut(s) 558, 786, 857, 1002, 1553, 1664, 1995, 2012, 2085
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.