RchiOBHm_Chr4g0425341

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000660
Physical Location & Seq
Forward (+)
50947116 .. 50949692
2577 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39450

Sequence Viewer

Length: 792 bp
ATGGAAATGGAGAGAAGCAGCTTAAGTATAATGATGATATATAATGTTAATGATGTTGCTGTTAAGGTATTCTTCAAGCAAGAATATCCAGATGATGTATCTCTTATGAAAAGGCTTCGACATCCCAACGTTCTGCTTTTCATGGGAGCTGTGACTTCACCTCAGCGTCTCTGCATCATCACAGAGTTCTTCCCACGTGGAAGTTTGTTTCAATTGCTACATAAGAACAAGTCAAACCCAGACTGGAGACAACGTATTCAAATGGCTTTACATATAGTGAGTATTGAGTTTTCCCCATCCCCGAACAAAAGAAAGAAAAAAACATATGATCACAGTGCACAAGGCATGAATTATCTTCATCATTTCAACCCCCCTATTATTCATCGAGATCCGAAGTCTTCAAATCTCCTAGTTGATAAGAACTGGACTGTCAAGGTTGGTGATTTTGGTCTGGCACGTCTCAAGCATGAAACATTTCTCTTTACCAAGACTGGGAAGGGGACGGTTCAATGGTTGGCACCAGAAGTTCTGTGTAATGAATTCTCAGATGAGAAGTCTGATGTATACAGCTATGGGGTGATATTGTGGGAGCTCGCTACTGAGAAGATTCCTTGGGACGATCTAAATGCAATGCAGGTGATTGGAGCTGTAGGGTTCAGGAAGGAAAGGCTAGAGATCCCAAAAGATGTGGATCCACAATGGGCTTCTATGATTGAGAGCTGTTGGCACAGGTGTGATCCAGCTCGCCGGCCAACATTTCATGAATTACTGGAAAAACTTAAAGCGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS PAS_9 Pkinase_Tyr

Protein Analysis

263

Amino Acids

30.67

Weight (kDa)

8.94

Isoelectric Point (pI)

40.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 19 - 260 2.7e-49 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 25 - 258 7.6e-39 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 625
Acc36I ACCTGC 1 cut(s) 625
AccB1I GGYRCC 1 cut(s) 517
AccI GTMKAC 1 cut(s) 564
AclI AACGTT 1 cut(s) 129
AclWI GGATC 5 cut(s) 383, 670, 686, 699, 731
AcoI YGGCCR 1 cut(s) 749
AcsI RAATTY 1 cut(s) 539
AcvI CACGTG 1 cut(s) 197
AfiI CCNNNNNNNGG 1 cut(s) 492
AflII CTTAAG 1 cut(s) 22
AgsI TTSAA 6 cut(s) 76, 212, 260, 367, 402, 509
AjiI CACGTC 1 cut(s) 458
AleI CACNNNNGTG 1 cut(s) 732
AluBI AGCT 7 cut(s) 21, 149, 570, 592, 647, 720, 743
AluI AGCT 7 cut(s) 21, 149, 570, 592, 647, 720, 743
Alw21I GWGCWC 2 cut(s) 340, 594
Alw26I GTCTC 3 cut(s) 173, 241, 464
Alw44I GTGCAC 1 cut(s) 336
AlwI GGATC 5 cut(s) 383, 670, 686, 699, 731
AoxI GGCC 1 cut(s) 749
ApaLI GTGCAC 1 cut(s) 336
ApeKI GCWGC 1 cut(s) 18
ApoI RAATTY 1 cut(s) 539
Asp700I GAANNNNTTC 1 cut(s) 474
AsuHPI GGTGA 4 cut(s) 150, 452, 589, 649
BaeGI GKGCMC 1 cut(s) 340
BamHI GGATCC 1 cut(s) 691
BanI GGYRCC 1 cut(s) 517
BanII GRGCYC 1 cut(s) 594
BbrPI CACGTG 1 cut(s) 197
BbsI GAAGAC 1 cut(s) 390
Bbv12I GWGCWC 2 cut(s) 340, 594
BbvCI CCTCAGC 1 cut(s) 162
BbvI GCAGC 1 cut(s) 30
BccI CCATC 1 cut(s) 304
BcgI CGANNNNNNTGC 2 cut(s) 608, 642
BclI TGATCA 1 cut(s) 328
BcoDI GTCTC 3 cut(s) 173, 241, 464
BfaI CTAG 2 cut(s) 410, 671
BfmI CTRYAG 1 cut(s) 648
BfrI CTTAAG 1 cut(s) 22
BfuAI ACCTGC 1 cut(s) 625
BisI GCNGC 1 cut(s) 19
BlsI GCNGC 1 cut(s) 20
BmgBI CACGTC 1 cut(s) 458
BmiI GGNNCC 2 cut(s) 519, 693
BmrI ACTGGG 1 cut(s) 501
BmsI GCATC 1 cut(s) 183
BmuI ACTGGG 1 cut(s) 501
BpiI GAAGAC 1 cut(s) 390
BpmI CTGGAG 1 cut(s) 265
Bpu10I CCTNAGC 1 cut(s) 162
BpuEI CTTGAG 1 cut(s) 446
BsaAI YACGTR 1 cut(s) 197
BsaBI GATNNNNATC 1 cut(s) 690
BsaJI CCNNGG 1 cut(s) 611
BsaXI ACNNNNNCTCC 2 cut(s) 238, 268
Bsc4I CCNNNNNNNGG 1 cut(s) 492
Bse118I RCCGGY 1 cut(s) 747
Bse1I ACTGG 4 cut(s) 248, 428, 496, 774
Bse3DI GCAATG 1 cut(s) 636
Bse8I GATNNNNATC 1 cut(s) 690
BseDI CCNNGG 1 cut(s) 611
BseGI GGATG 2 cut(s) 121, 296
BseJI GATNNNNATC 1 cut(s) 690
BseLI CCNNNNNNNGG 1 cut(s) 492
BseMI GCAATG 1 cut(s) 636
BseMII CTCAG 3 cut(s) 176, 558, 591
BseNI ACTGG 4 cut(s) 248, 428, 496, 774
BseSI GKGCMC 1 cut(s) 340
BseXI GCAGC 1 cut(s) 30
BshFI GGCC 1 cut(s) 751
BshNI GGYRCC 1 cut(s) 517
BsiHKAI GWGCWC 2 cut(s) 340, 594
BsiSI CCGG 1 cut(s) 748
BslFI GGGAC 2 cut(s) 514, 629
BslI CCNNNNNNNGG 1 cut(s) 492
BsmAI GTCTC 3 cut(s) 173, 241, 464
BsmBI CGTCTC 2 cut(s) 173, 464
BsmFI GGGAC 2 cut(s) 514, 629
BsnI GGCC 1 cut(s) 751
Bsp1286I GDGCHC 2 cut(s) 340, 594
Bsp143I GATC 6 cut(s) 328, 388, 619, 675, 691, 736
BspANI GGCC 1 cut(s) 751
BspCNI CTCAG 3 cut(s) 175, 557, 592
BspHI TCATGA 1 cut(s) 760
BspLI GGNNCC 2 cut(s) 519, 693
BspMI ACCTGC 1 cut(s) 625
BspPI GGATC 5 cut(s) 383, 670, 686, 699, 731
BspT107I GGYRCC 1 cut(s) 517
BspTI CTTAAG 1 cut(s) 22
BsrDI GCAATG 1 cut(s) 636
BsrFI RCCGGY 1 cut(s) 747
BsrI ACTGG 4 cut(s) 248, 428, 496, 774
BssAI RCCGGY 1 cut(s) 747
BssECI CCNNGG 1 cut(s) 611
BssMI GATC 6 cut(s) 328, 388, 619, 675, 691, 736
BssNAI GTATAC 1 cut(s) 565
BssT1I CCWWGG 1 cut(s) 611
Bst1107I GTATAC 1 cut(s) 565
Bst4CI ACNGT 3 cut(s) 335, 430, 505
BstAFI CTTAAG 1 cut(s) 22
BstBAI YACGTR 1 cut(s) 197
BstC8I GCNNGC 3 cut(s) 594, 745, 749
BstDEI CTNAG 3 cut(s) 162, 544, 600
BstF5I GGATG 2 cut(s) 121, 296
BstKTI GATC 6 cut(s) 331, 391, 622, 678, 694, 739
BstMAI GTCTC 3 cut(s) 173, 241, 464
BstMBI GATC 6 cut(s) 328, 388, 619, 675, 691, 736
BstSFI CTRYAG 1 cut(s) 648
BstSLI GKGCMC 1 cut(s) 340
BstV1I GCAGC 1 cut(s) 30
BstV2I GAAGAC 1 cut(s) 390
BstX2I RGATCY 3 cut(s) 388, 675, 691
BstYI RGATCY 3 cut(s) 388, 675, 691
BstZ17I GTATAC 1 cut(s) 565
BsuRI GGCC 1 cut(s) 751
BtrI CACGTC 1 cut(s) 458
BtsCI GGATG 2 cut(s) 121, 296
BtsIMutI CAGTG 1 cut(s) 340
BveI ACCTGC 1 cut(s) 625
Cac8I GCNNGC 3 cut(s) 594, 745, 749
CciI TCATGA 1 cut(s) 760
Cfr10I RCCGGY 1 cut(s) 747
CseI GACGC 1 cut(s) 155
CspCI CAANNNNNGTGG 2 cut(s) 669, 704
CviAII CATG 4 cut(s) 142, 346, 467, 761
DdeI CTNAG 3 cut(s) 162, 544, 600
DpnI GATC 6 cut(s) 330, 390, 621, 677, 693, 738
DpnII GATC 6 cut(s) 328, 388, 619, 675, 691, 736
EaeI YGGCCR 1 cut(s) 749
Ecl136II GAGCTC 1 cut(s) 592
Eco130I CCWWGG 1 cut(s) 611
Eco24I GRGCYC 1 cut(s) 594
Eco53kI GAGCTC 1 cut(s) 592
Eco72I CACGTG 1 cut(s) 197
EcoICRI GAGCTC 1 cut(s) 592
EcoRI GAATTC 1 cut(s) 539
EcoT14I CCWWGG 1 cut(s) 611
EcoT38I GRGCYC 1 cut(s) 594
ErhI CCWWGG 1 cut(s) 611
Esp3I CGTCTC 2 cut(s) 173, 464
FaeI CATG 4 cut(s) 145, 349, 470, 764
FalI AAGNNNNNCTT 2 cut(s) 56, 88
FaqI GGGAC 2 cut(s) 514, 629
FatI CATG 4 cut(s) 141, 345, 466, 760
FauNDI CATATG 1 cut(s) 325
FbaI TGATCA 1 cut(s) 328
FblI GTMKAC 1 cut(s) 564
Fnu4HI GCNGC 1 cut(s) 19
FokI GGATG 2 cut(s) 108, 283
FriOI GRGCYC 1 cut(s) 594
Fsp4HI GCNGC 1 cut(s) 19
FspBI CTAG 2 cut(s) 410, 671
GluI GCNGC 1 cut(s) 19
GsuI CTGGAG 1 cut(s) 265
HaeIII GGCC 1 cut(s) 751
HapII CCGG 1 cut(s) 748
HgaI GACGC 1 cut(s) 155
Hin1II CATG 4 cut(s) 145, 349, 470, 764
HinfI GANTC 1 cut(s) 607
HpaII CCGG 1 cut(s) 748
HphI GGTGA 4 cut(s) 150, 452, 589, 649
Hpy166II GTNNAC 2 cut(s) 338, 565
Hpy188I TCNGA 3 cut(s) 393, 547, 559
Hpy188III TCNNGA 4 cut(s) 89, 386, 658, 761
Hpy8I GTNNAC 2 cut(s) 338, 565
HpyAV CCTTC 2 cut(s) 490, 655
HpyCH4III ACNGT 3 cut(s) 335, 430, 505
HpyCH4IV ACGT 4 cut(s) 129, 196, 253, 457
HpyCH4V TGCA 4 cut(s) 174, 338, 629, 634
HpyF3I CTNAG 3 cut(s) 162, 544, 600
HpySE526I ACGT 4 cut(s) 129, 196, 253, 457
Hsp92II CATG 4 cut(s) 145, 349, 470, 764
KroI GCCGGC 1 cut(s) 747
KroNI GCCGGC 1 cut(s) 749
Ksp22I TGATCA 1 cut(s) 328
Kzo9I GATC 6 cut(s) 328, 388, 619, 675, 691, 736
LmnI GCTCC 3 cut(s) 146, 589, 644
Lsp1109I GCAGC 1 cut(s) 30
LweI GCATC 1 cut(s) 183
MaeI CTAG 2 cut(s) 410, 671
MaeII ACGT 4 cut(s) 129, 196, 253, 457
MaeIII GTNAC 1 cut(s) 151
MalI GATC 6 cut(s) 330, 390, 621, 677, 693, 738
MboI GATC 6 cut(s) 328, 388, 619, 675, 691, 736
MboII GAAGA 5 cut(s) 64, 181, 347, 390, 616
MfeI CAATTG 1 cut(s) 212
MflI RGATCY 3 cut(s) 388, 675, 691
MhlI GDGCHC 2 cut(s) 340, 594
MluCI AATT 4 cut(s) 212, 349, 539, 764
MnlI CCTC 1 cut(s) 171
MroNI GCCGGC 1 cut(s) 747
MroXI GAANNNNTTC 1 cut(s) 474
MseI TTAA 4 cut(s) 23, 48, 63, 780
MslI CAYNNNNRTG 1 cut(s) 732
MspCI CTTAAG 1 cut(s) 22
MspI CCGG 1 cut(s) 748
MunI CAATTG 1 cut(s) 212
NaeI GCCGGC 1 cut(s) 749
NdeI CATATG 1 cut(s) 325
NdeII GATC 6 cut(s) 328, 388, 619, 675, 691, 736
NgoMIV GCCGGC 1 cut(s) 747
NlaIII CATG 4 cut(s) 145, 349, 470, 764
NlaIV GGNNCC 2 cut(s) 519, 693
NmuCI GTSAC 1 cut(s) 151
OliI CACNNNNGTG 1 cut(s) 732
PagI TCATGA 1 cut(s) 760
PaqCI CACCTGC 1 cut(s) 625
PdiI GCCGGC 1 cut(s) 749
PdmI GAANNNNTTC 1 cut(s) 474
PfeI GAWTC 1 cut(s) 607
PkrI GCNGC 1 cut(s) 20
PmaCI CACGTG 1 cut(s) 197
PmlI CACGTG 1 cut(s) 197
Ppu21I YACGTR 1 cut(s) 197
Psp124BI GAGCTC 1 cut(s) 594
Psp1406I AACGTT 1 cut(s) 129
PspCI CACGTG 1 cut(s) 197
PspN4I GGNNCC 2 cut(s) 519, 693
PsuI RGATCY 3 cut(s) 388, 675, 691
RseI CAYNNNNRTG 1 cut(s) 732
SacI GAGCTC 1 cut(s) 594
SaqAI TTAA 4 cut(s) 23, 48, 63, 780
SatI GCNGC 1 cut(s) 19
Sau3AI GATC 6 cut(s) 328, 388, 619, 675, 691, 736
SduI GDGCHC 2 cut(s) 340, 594
SfaNI GCATC 1 cut(s) 183
SfcI CTRYAG 1 cut(s) 648
SmiMI CAYNNNNRTG 1 cut(s) 732
SmlI CTYRAG 2 cut(s) 22, 461
SmoI CTYRAG 2 cut(s) 22, 461
Sse9I AATT 4 cut(s) 212, 349, 539, 764
SspMI CTAG 2 cut(s) 410, 671
SstI GAGCTC 1 cut(s) 594
StyI CCWWGG 1 cut(s) 611
TaaI ACNGT 3 cut(s) 335, 430, 505
TaiI ACGT 4 cut(s) 132, 199, 256, 460
TaqI TCGA 2 cut(s) 118, 385
TasI AATT 4 cut(s) 212, 349, 539, 764
TfiI GAWTC 1 cut(s) 607
Tru1I TTAA 4 cut(s) 23, 48, 63, 780
Tru9I TTAA 4 cut(s) 23, 48, 63, 780
TscAI CASTG 1 cut(s) 340
TseFI GTSAC 1 cut(s) 151
TseI GCWGC 1 cut(s) 18
Tsp45I GTSAC 1 cut(s) 151
TspDTI ATGAA 9 cut(s) 122, 130, 347, 362, 371, 483, 552, 749, 777
TspRI CASTG 1 cut(s) 340
Vha464I CTTAAG 1 cut(s) 22
VneI GTGCAC 1 cut(s) 336
XapI RAATTY 1 cut(s) 539
XmiI GTMKAC 1 cut(s) 564
XmnI GAANNNNTTC 1 cut(s) 474
XspI CTAG 2 cut(s) 410, 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.