Rh4DG261500

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000660
Physical Location & Seq
Forward (+)
47926215 .. 47933378
7164 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG261500.1

Sequence Viewer

Length: 2292 bp
ATGGACACTCCGCCGCCGCCGGAGGAGCTGCTGAAGAAGATCCAGGTGCTGGAGGAGGGTCAGGAGCACCTGAAGCAGGAGATGTCCAAGCTCCGGAACAACTCCGATGCTAAGTCCGAGCACCCGAGGGCCCACTCGGTCTCGCCGCAGCGGTCCAGATTCTCGTCTTTGCCGAAGAGGAGAGTCGCCGGAGCCGACGCGGTGGGTTGGAAGAAAGGGTCTACTTCGTTTCGGCATTTTTCGCCGCTGCAGAGGGAGAGCCGCAGCCATGATCCTCCGACTGGCGGCAGCGGCCGCGCCGTCGATGATTTTAGAGGTGGAGGTGGTAGCGGGCCGTCGGCGGTGAACTTTACTGATAGGCAGTATCTGAATATATTGCAGTCCATTGGGCAGTCTGTGCATATTTTTGATCCCCATGGTCGTATAATTTACTGGAACAAAACAGCTGAAGCTATGTATGGTTATTCAGCGGCAGAGGCCCTGGGACGGGATCCCATTGAGTTCTTAACAGATCCCCAGGACCATGTTGTAGGACATAATATAATCCAAAGAGTCACCAAGGGGGAGAGCTGGACAGGGCACTTCCCTGTCAAGAACAAAAAGGGGGAGAGATTTACAGCTACTATAACGAATACTCCATTCTATGATGATGATGGCACTTTTATTGGGGTTATTTGTGTGTCAAGTGATACGAGGCCATTTCAAGAAATGAGGACGCACCCTGAACCAGATTCAAGCTTCAGTCGTACTAAAGCTACTGTTACAACTAAACTTGGTCTTGATCCTCAGCAGCCTCTGCAAACTGCAATTGCATCAAAAATTACAAATTTGGCATCCAAGATGAGCAACAAAGTCAAGTCGAAAATTCGGGCAGGAGAAAACTATATGGATCGGGAAGGTGGGAGTGTAGATAGTCATCATTCTGATCATGGTTCGGACTATGACCATCGGGAGGACGCAAATTCAAGTGGGGCTAGCACACCTAGAGGAGATATAGCCCCATCTCCTTTTGGTGTGTTTTCCCAGATGGATGAAAAGTCTCCAGGAAAACCTTCCAGAGATTCTGGCGATGAGAGTGAAGGAAAACCGCCAATGCACAAGGTAATATCCTCAAAGGCAGAAGCATGGATTGGTAAGAAGGGGATAACTTGGCCATGGAAAGGAAATGAGCGAGAAGGGTCAGAGGTTAAGACCCCTCGTTTTCTTTGGCCTTGGTTGCAAAATGATCAAGAGAATGATCCAGTTCCTCAGAAGAATAATTGTGGTTTAAAACCTGACAATTACACGACTGAAAGTAATCGACATGCAACTAATGAGGCTTCAGGATCCTGGACCTCCTCATTTAATGTTAACAGCACAAGCAGTGCCAGCAGCTGTGGAAGCACCAGCAGTACTGTGAATAAGGTAGACGTGGACACTGATTGCTTGGATTACGAAATCTTGTGGGAAGACTTGATGATTGGAGAACAGATTGGGCAAGGTTCATGTGGAACTGTATACCATGGTCTGTGGTACGGATCAGATGTTGCTGTCAAGGTATTCTCCAGGCAAGAATATTCAGAAGATGTGATACTTTCCTTCAGACAAGAGGTATCTCTTATGAAAAGGCTTCGACATCCAAATGTTCTGCTTTTCATGGGAGCTGTGACTTCTCCCGAGCGTCTTTGCATCGTAACAGAGTTCCTCCCACGTGGAAGTTTGTTTCGGTTACTACAAAGAAGCACAGCAAAACTAGACTGGAGACGACGTGTTCTTATGGCTCTGGATATAGCACGAGGCATGAATTATCTTCATCATTTCAACCCTCCAATCATCCATCGAGATCTGAAGTCTTCAAATCTCCTAGTTGATAAGAACTGGACTGTCAAGGTTGGTGATTTTGGTCTTTCACGTCTAAAGCATGAAACATTTCTTACAACCAAGACTGGGAGGGGAACGCCCCAATGGATGGCACCAGAAGTTCTACGCAATGAACCCTCAGATGAGAAGTCGGATGTATATAGCTATGGAGTGATATTGTGGGAGCTTGTTACTGAAAAGATCCCTTGGGACAATCTAAACTCAATGCAGGTGATTGGAGCTGTAGGGTTCATGAACCAAAGACTAGAGATCCCAAAAGATATTGACCCGCAATGGATTTCTTTAATTGAGAGCTGCTGGCAAAGTGATCCTGCTAGCCGGCCAACATTTCAAGAGCTGCTGGAAAAGCTTCGAGATCTGCAGAGACAATATGCTCTTCAATTCCAAGCAGCGCGGTCCGCCGCAAGTGATAGCACCCAAAAAGAACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS PAS_9 Pkinase_Tyr

Protein Analysis

763

Amino Acids

85.53

Weight (kDa)

6.66

Isoelectric Point (pI)

50.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS PF00989 121 - 230 1.3e-13 PAS fold
PAS_8 PF13188 121 - 174 5.9e-06 PAS domain
PAS_4 PF08448 127 - 231 7.1e-13 PAS fold
PAS_9 PF13426 135 - 230 1.1e-12 PAS domain
PAS_3 PF08447 142 - 227 9.2e-07 PAS fold
PK_Tyr_Ser-Thr PF07714 486 - 737 8.2e-68 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 488 - 735 1.4e-57 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2059
Acc36I ACCTGC 1 cut(s) 2059
AccB1I GGYRCC 1 cut(s) 1951
AccB7I CCANNNNNTGG 2 cut(s) 49, 1948
AccI GTMKAC 3 cut(s) 221, 1407, 1497
AccII CGCG 3 cut(s) 200, 297, 2254
AccIII TCCGGA 1 cut(s) 93
AcoI YGGCCR 3 cut(s) 292, 1151, 2180
AcsI RAATTY 3 cut(s) 826, 864, 961
AcuI CTGAAG 7 cut(s) 53, 92, 468, 724, 1305, 1564, 1847
AcvI CACGTG 1 cut(s) 1691
AfaI GTAC 3 cut(s) 748, 1393, 1514
AflIII ACRYGT 1 cut(s) 1747
AgsI TTSAA 7 cut(s) 704, 735, 966, 1801, 1836, 2192, 2240
AjiI CACGTC 3 cut(s) 1411, 1748, 1892
AjnI CCWGG 6 cut(s) 42, 480, 516, 1042, 1328, 1544
AjuI GAANNNNNNNTTGG 2 cut(s) 1113, 1145
Alw21I GWGCWC 2 cut(s) 69, 123
Alw26I GTCTC 4 cut(s) 145, 1044, 1735, 2218
AlwNI CAGNNNCTG 4 cut(s) 49, 367, 796, 1374
Ama87I CYCGRG 2 cut(s) 124, 1655
Aor13HI TCCGGA 1 cut(s) 93
AoxI GGCC 8 cut(s) 129, 292, 332, 477, 695, 1151, 1208, 2180
ApaI GGGCCC 1 cut(s) 133
ApoI RAATTY 3 cut(s) 826, 864, 961
Asp700I GAANNNNTTC 3 cut(s) 1051, 1908, 2208
AspLEI GCGC 2 cut(s) 299, 2254
AspS9I GGNCC 8 cut(s) 129, 130, 153, 332, 478, 520, 1332, 2256
AsuHPI GGTGA 4 cut(s) 355, 547, 1886, 2083
AsuNHI GCTAGC 2 cut(s) 974, 2174
AvaI CYCGRG 2 cut(s) 124, 1655
AvaII GGWCC 4 cut(s) 153, 520, 1332, 2256
BaeGI GKGCMC 2 cut(s) 133, 582
BalI TGGCCA 1 cut(s) 1153
BamHI GGATCC 2 cut(s) 490, 1325
BanI GGYRCC 1 cut(s) 1951
BanII GRGCYC 1 cut(s) 133
BarI GAAGNNNNNNTAC 2 cut(s) 1554, 1586
BauI CACGAG 1 cut(s) 1773
BbrPI CACGTG 1 cut(s) 1691
BbsI GAAGAC 2 cut(s) 1455, 1824
Bbv12I GWGCWC 2 cut(s) 69, 123
BbvCI CCTCAGC 1 cut(s) 786
BccI CCATC 6 cut(s) 647, 954, 1009, 1021, 1824, 1942
BceAI ACGGC 2 cut(s) 284, 319
BciT130I CCWGG 6 cut(s) 44, 482, 518, 1044, 1330, 1546
BclI TGATCA 2 cut(s) 925, 1225
BcoDI GTCTC 4 cut(s) 145, 1044, 1735, 2218
BfaI CTAG 6 cut(s) 975, 984, 1733, 1844, 2105, 2175
BfmI CTRYAG 4 cut(s) 248, 2082, 2219, 2288
BfuAI ACCTGC 1 cut(s) 2059
BglII AGATCT 2 cut(s) 1822, 2215
BmcAI AGTACT 1 cut(s) 1393
Bme1390I CCNGG 6 cut(s) 44, 482, 518, 1044, 1330, 1546
Bme18I GGWCC 4 cut(s) 153, 520, 1332, 2256
BmeT110I CYCGRG 2 cut(s) 124, 1655
BmgBI CACGTC 3 cut(s) 1411, 1748, 1892
BmgT120I GGNCC 8 cut(s) 129, 130, 153, 332, 478, 520, 1332, 2256
BmiI GGNNCC 5 cut(s) 131, 193, 492, 1327, 1953
BmrFI CCNGG 6 cut(s) 44, 482, 518, 1044, 1330, 1546
BmrI ACTGGG 1 cut(s) 1935
BmsI GCATC 4 cut(s) 97, 821, 842, 1677
BmtI GCTAGC 2 cut(s) 978, 2178
BmuI ACTGGG 1 cut(s) 1935
BpiI GAAGAC 2 cut(s) 1455, 1824
BpmI CTGGAG 4 cut(s) 71, 1026, 1528, 1759
Bpu10I CCTNAGC 1 cut(s) 786
BsaAI YACGTR 1 cut(s) 1691
BsaBI GATNNNNATC 1 cut(s) 915
BsaI GGTCTC 1 cut(s) 145
BsaWI WCCGGW 1 cut(s) 93
BsaXI ACNNNNNCTCC 4 cut(s) 619, 649, 1732, 1762
Bse118I RCCGGY 1 cut(s) 2178
Bse1I ACTGG 6 cut(s) 286, 437, 1241, 1742, 1862, 1930
Bse3DI GCAATG 2 cut(s) 1975, 2138
Bse8I GATNNNNATC 1 cut(s) 915
BseAI TCCGGA 1 cut(s) 93
BseBI CCWGG 6 cut(s) 44, 482, 518, 1044, 1330, 1546
BseGI GGATG 6 cut(s) 833, 1036, 1615, 1812, 1953, 1999
BseJI GATNNNNATC 1 cut(s) 915
BseMI GCAATG 2 cut(s) 1975, 2138
BseMII CTCAG 3 cut(s) 800, 1262, 1992
BseNI ACTGG 6 cut(s) 286, 437, 1241, 1742, 1862, 1930
BseRI GAGGAG 5 cut(s) 38, 68, 193, 1002, 1327
BseSI GKGCMC 2 cut(s) 133, 582
BseX3I CGGCCG 1 cut(s) 292
Bsh1236I CGCG 3 cut(s) 200, 297, 2254
Bsh1285I CGRYCG 1 cut(s) 295
BshFI GGCC 8 cut(s) 131, 294, 334, 479, 697, 1153, 1210, 2182
BshNI GGYRCC 1 cut(s) 1951
BsiEI CGRYCG 1 cut(s) 295
BsiHKAI GWGCWC 2 cut(s) 69, 123
BsiHKCI CYCGRG 2 cut(s) 124, 1655
BsiSI CCGG 4 cut(s) 20, 94, 189, 2179
BslFI GGGAC 2 cut(s) 498, 2063
BsmAI GTCTC 4 cut(s) 145, 1044, 1735, 2218
BsmBI CGTCTC 1 cut(s) 1735
BsmFI GGGAC 2 cut(s) 498, 2063
BsnI GGCC 8 cut(s) 131, 294, 334, 479, 697, 1153, 1210, 2182
Bso31I GGTCTC 1 cut(s) 145
BsoBI CYCGRG 2 cut(s) 124, 1655
Bsp120I GGGCCC 1 cut(s) 129
Bsp1286I GDGCHC 4 cut(s) 69, 123, 133, 582
Bsp13I TCCGGA 1 cut(s) 93
Bsp19I CCATGG 3 cut(s) 415, 1154, 1501
BspANI GGCC 8 cut(s) 131, 294, 334, 479, 697, 1153, 1210, 2182
BspCNI CTCAG 3 cut(s) 799, 1261, 1991
BspEI TCCGGA 1 cut(s) 93
BspFNI CGCG 3 cut(s) 200, 297, 2254
BspHI TCATGA 1 cut(s) 2091
BspLI GGNNCC 5 cut(s) 131, 193, 492, 1327, 1953
BspMAI CTGCAG 2 cut(s) 252, 2223
BspMI ACCTGC 1 cut(s) 2059
BspOI GCTAGC 2 cut(s) 978, 2178
BspQI GCTCTTC 1 cut(s) 2241
BspT107I GGYRCC 1 cut(s) 1951
BspTNI GGTCTC 1 cut(s) 145
BsrDI GCAATG 2 cut(s) 1975, 2138
BsrFI RCCGGY 1 cut(s) 2178
BsrI ACTGG 6 cut(s) 286, 437, 1241, 1742, 1862, 1930
BssAI RCCGGY 1 cut(s) 2178
BssNAI GTATAC 1 cut(s) 1498
BssSI CACGAG 1 cut(s) 1773
BssT1I CCWWGG 6 cut(s) 415, 558, 1154, 1211, 1501, 2045
Bst1107I GTATAC 1 cut(s) 1498
Bst2BI CACGAG 1 cut(s) 1773
Bst2UI CCWGG 6 cut(s) 44, 482, 518, 1044, 1330, 1546
Bst4CI ACNGT 5 cut(s) 760, 1396, 1495, 1864, 2289
Bst6I CTCTTC 2 cut(s) 170, 2241
BstAPI GCANNNNNTGC 2 cut(s) 397, 796
BstBAI YACGTR 1 cut(s) 1691
BstC8I GCNNGC 6 cut(s) 332, 976, 1369, 2159, 2176, 2180
BstDEI CTNAG 4 cut(s) 111, 786, 1248, 1978
BstDSI CCRYGG 3 cut(s) 415, 1154, 1501
BstENI CCTNNNNNAGG 1 cut(s) 74
BstF5I GGATG 6 cut(s) 833, 1036, 1615, 1812, 1953, 1999
BstFNI CGCG 3 cut(s) 200, 297, 2254
BstHHI GCGC 2 cut(s) 299, 2254
BstMAI GTCTC 4 cut(s) 145, 1044, 1735, 2218
BstMCI CGRYCG 1 cut(s) 295
BstNI CCWGG 6 cut(s) 44, 482, 518, 1044, 1330, 1546
BstNSI RCATGY 1 cut(s) 1307
BstSCI CCNGG 6 cut(s) 42, 480, 516, 1042, 1328, 1544
BstSFI CTRYAG 4 cut(s) 248, 2082, 2219, 2288
BstSLI GKGCMC 2 cut(s) 133, 582
BstUI CGCG 3 cut(s) 200, 297, 2254
BstV2I GAAGAC 2 cut(s) 1455, 1824
BstX2I RGATCY 8 cut(s) 39, 490, 511, 1325, 1822, 2040, 2109, 2215
BstYI RGATCY 8 cut(s) 39, 490, 511, 1325, 1822, 2040, 2109, 2215
BstZ17I GTATAC 1 cut(s) 1498
BstZI CGGCCG 1 cut(s) 292
BsuRI GGCC 8 cut(s) 131, 294, 334, 479, 697, 1153, 1210, 2182
BtgI CCRYGG 3 cut(s) 415, 1154, 1501
BtgZI GCGATG 1 cut(s) 1083
BtrI CACGTC 3 cut(s) 1411, 1748, 1892
BtsCI GGATG 6 cut(s) 833, 1036, 1615, 1812, 1953, 1999
BtsI GCAGTG 1 cut(s) 1369
BtsIMutI CAGTG 2 cut(s) 1369, 1416
BveI ACCTGC 1 cut(s) 2059
Cac8I GCNNGC 6 cut(s) 332, 976, 1369, 2159, 2176, 2180
CaiI CAGNNNCTG 4 cut(s) 49, 367, 796, 1374
CciI TCATGA 1 cut(s) 2091
CciNI GCGGCCGC 1 cut(s) 292
CfoI GCGC 2 cut(s) 299, 2254
Cfr10I RCCGGY 1 cut(s) 2178
Cfr13I GGNCC 8 cut(s) 129, 130, 153, 332, 478, 520, 1332, 2256
CpoI CGGWCCG 1 cut(s) 2256
CseI GACGC 4 cut(s) 206, 724, 965, 1649
Csp6I GTAC 3 cut(s) 747, 1392, 1513
CspI CGGWCCG 1 cut(s) 2256
CviQI GTAC 3 cut(s) 747, 1392, 1513
DdeI CTNAG 4 cut(s) 111, 786, 1248, 1978
DraI TTTAAA 1 cut(s) 1269
EaeI YGGCCR 3 cut(s) 292, 1151, 2180
EagI CGGCCG 1 cut(s) 292
Eam1104I CTCTTC 2 cut(s) 170, 2241
EarI CTCTTC 2 cut(s) 170, 2241
EciI GGCGGA 1 cut(s) 2248
EclXI CGGCCG 1 cut(s) 292
Eco130I CCWWGG 6 cut(s) 415, 558, 1154, 1211, 1501, 2045
Eco24I GRGCYC 1 cut(s) 133
Eco31I GGTCTC 1 cut(s) 145
Eco47I GGWCC 4 cut(s) 153, 520, 1332, 2256
Eco52I CGGCCG 1 cut(s) 292
Eco57I CTGAAG 7 cut(s) 53, 92, 468, 724, 1305, 1564, 1847
Eco72I CACGTG 1 cut(s) 1691
Eco88I CYCGRG 2 cut(s) 124, 1655
EcoNI CCTNNNNNAGG 1 cut(s) 74
EcoO109I RGGNCCY 2 cut(s) 129, 478
EcoRII CCWGG 6 cut(s) 42, 480, 516, 1042, 1328, 1544
EcoT14I CCWWGG 6 cut(s) 415, 558, 1154, 1211, 1501, 2045
EcoT38I GRGCYC 1 cut(s) 133
ErhI CCWWGG 6 cut(s) 415, 558, 1154, 1211, 1501, 2045
Esp3I CGTCTC 1 cut(s) 1735
FaqI GGGAC 2 cut(s) 498, 2063
FauI CCCGC 2 cut(s) 323, 2136
FbaI TGATCA 2 cut(s) 925, 1225
FblI GTMKAC 3 cut(s) 221, 1407, 1497
FokI GGATG 6 cut(s) 820, 1043, 1602, 1799, 1960, 2006
FriOI GRGCYC 1 cut(s) 133
FspBI CTAG 6 cut(s) 975, 984, 1733, 1844, 2105, 2175
GlaI GCGC 2 cut(s) 298, 2253
GsuI CTGGAG 4 cut(s) 71, 1026, 1528, 1759
HaeIII GGCC 8 cut(s) 131, 294, 334, 479, 697, 1153, 1210, 2182
HapII CCGG 4 cut(s) 20, 94, 189, 2179
HgaI GACGC 4 cut(s) 206, 724, 965, 1649
HhaI GCGC 2 cut(s) 299, 2254
Hin6I GCGC 2 cut(s) 297, 2252
HinP1I GCGC 2 cut(s) 297, 2252
HincII GTYRAC 1 cut(s) 1351
HindII GTYRAC 1 cut(s) 1351
HindIII AAGCTT 2 cut(s) 736, 2207
HinfI GANTC 5 cut(s) 159, 183, 552, 731, 1061
HpaI GTTAAC 1 cut(s) 1351
HpaII CCGG 4 cut(s) 20, 94, 189, 2179
HphI GGTGA 4 cut(s) 355, 547, 1886, 2083
Hpy166II GTNNAC 6 cut(s) 222, 346, 1351, 1408, 1414, 1498
Hpy8I GTNNAC 6 cut(s) 222, 346, 1351, 1408, 1414, 1498
Hpy99I CGWCG 4 cut(s) 200, 305, 340, 1749
HpyAV CCTTC 6 cut(s) 890, 1062, 1073, 1132, 1169, 1588
HpyCH4III ACNGT 5 cut(s) 760, 1396, 1495, 1864, 2289
HpyCH4IV ACGT 4 cut(s) 1410, 1690, 1747, 1891
HpyF3I CTNAG 4 cut(s) 111, 786, 1248, 1978
HpySE526I ACGT 4 cut(s) 1410, 1690, 1747, 1891
HspAI GCGC 2 cut(s) 297, 2252
Kpn2I TCCGGA 1 cut(s) 93
KroI GCCGGC 1 cut(s) 2178
KroNI GCCGGC 1 cut(s) 2180
Ksp22I TGATCA 2 cut(s) 925, 1225
KspAI GTTAAC 1 cut(s) 1351
LguI GCTCTTC 1 cut(s) 2241
LmnI GCTCC 7 cut(s) 25, 64, 96, 191, 1640, 2023, 2078
LweI GCATC 4 cut(s) 97, 821, 842, 1677
MaeI CTAG 6 cut(s) 975, 984, 1733, 1844, 2105, 2175
MaeII ACGT 4 cut(s) 1410, 1690, 1747, 1891
MaeIII GTNAC 6 cut(s) 553, 760, 1645, 1672, 1707, 2029
MfeI CAATTG 1 cut(s) 807
MflI RGATCY 8 cut(s) 39, 490, 511, 1325, 1822, 2040, 2109, 2215
MhlI GDGCHC 4 cut(s) 69, 123, 133, 582
MlsI TGGCCA 1 cut(s) 1153
MluNI TGGCCA 1 cut(s) 1153
MlyI GAGTC 2 cut(s) 192, 561
MmeI TCCRAC 3 cut(s) 188, 302, 1971
Mox20I TGGCCA 1 cut(s) 1153
MroI TCCGGA 1 cut(s) 93
MroNI GCCGGC 1 cut(s) 2178
MroXI GAANNNNTTC 3 cut(s) 1051, 1908, 2208
MscI TGGCCA 1 cut(s) 1153
MseI TTAA 6 cut(s) 506, 1188, 1268, 1344, 1350, 2144
MslI CAYNNNNRTG 1 cut(s) 1620
Msp20I TGGCCA 1 cut(s) 1153
MspA1I CMGCKG 6 cut(s) 151, 247, 291, 446, 470, 1374
MspI CCGG 4 cut(s) 20, 94, 189, 2179
MspR9I CCNGG 6 cut(s) 44, 482, 518, 1044, 1330, 1546
MunI CAATTG 1 cut(s) 807
MvaI CCWGG 6 cut(s) 44, 482, 518, 1044, 1330, 1546
MvnI CGCG 3 cut(s) 200, 297, 2254
NaeI GCCGGC 1 cut(s) 2180
NcoI CCATGG 3 cut(s) 415, 1154, 1501
NgoMIV GCCGGC 1 cut(s) 2178
NheI GCTAGC 2 cut(s) 974, 2174
NlaIV GGNNCC 5 cut(s) 131, 193, 492, 1327, 1953
NmuCI GTSAC 2 cut(s) 553, 1645
NotI GCGGCCGC 1 cut(s) 292
NspI RCATGY 1 cut(s) 1307
PagI TCATGA 1 cut(s) 2091
PaqCI CACCTGC 1 cut(s) 2059
PasI CCCWGGG 1 cut(s) 481
PciSI GCTCTTC 1 cut(s) 2241
PcsI WCGNNNNNNNCGW 2 cut(s) 170, 192
PdiI GCCGGC 1 cut(s) 2180
PdmI GAANNNNTTC 3 cut(s) 1051, 1908, 2208
PfeI GAWTC 3 cut(s) 159, 731, 1061
PflMI CCANNNNNTGG 2 cut(s) 49, 1948
PfoI TCCNGGA 2 cut(s) 1042, 1328
PleI GAGTC 2 cut(s) 191, 560
PmaCI CACGTG 1 cut(s) 1691
PmlI CACGTG 1 cut(s) 1691
PpsI GAGTC 2 cut(s) 191, 560
Ppu21I YACGTR 1 cut(s) 1691
Psp6I CCWGG 6 cut(s) 42, 480, 516, 1042, 1328, 1544
PspCI CACGTG 1 cut(s) 1691
PspGI CCWGG 6 cut(s) 42, 480, 516, 1042, 1328, 1544
PspN4I GGNNCC 5 cut(s) 131, 193, 492, 1327, 1953
PspOMI GGGCCC 1 cut(s) 129
PspPI GGNCC 8 cut(s) 129, 130, 153, 332, 478, 520, 1332, 2256
PstI CTGCAG 2 cut(s) 252, 2223
PstNI CAGNNNCTG 4 cut(s) 49, 367, 796, 1374
PsuI RGATCY 8 cut(s) 39, 490, 511, 1325, 1822, 2040, 2109, 2215
PvuII CAGCTG 2 cut(s) 446, 1374
RsaI GTAC 3 cut(s) 748, 1393, 1514
RsaNI GTAC 3 cut(s) 747, 1392, 1513
RseI CAYNNNNRTG 1 cut(s) 1620
Rsr2I CGGWCCG 1 cut(s) 2256
RsrII CGGWCCG 1 cut(s) 2256
SapI GCTCTTC 1 cut(s) 2241
SaqAI TTAA 6 cut(s) 506, 1188, 1268, 1344, 1350, 2144
Sau96I GGNCC 8 cut(s) 129, 130, 153, 332, 478, 520, 1332, 2256
ScaI AGTACT 1 cut(s) 1393
SchI GAGTC 2 cut(s) 192, 561
ScrFI CCNGG 6 cut(s) 44, 482, 518, 1044, 1330, 1546
SduI GDGCHC 4 cut(s) 69, 123, 133, 582
SfaNI GCATC 4 cut(s) 97, 821, 842, 1677
SfcI CTRYAG 4 cut(s) 248, 2082, 2219, 2288
SinI GGWCC 4 cut(s) 153, 520, 1332, 2256
SmiMI CAYNNNNRTG 1 cut(s) 1620
SspI AATATT 1 cut(s) 1556
SspMI CTAG 6 cut(s) 975, 984, 1733, 1844, 2105, 2175
StyD4I CCNGG 6 cut(s) 42, 480, 516, 1042, 1328, 1544
StyI CCWWGG 6 cut(s) 415, 558, 1154, 1211, 1501, 2045
TaaI ACNGT 5 cut(s) 760, 1396, 1495, 1864, 2289
TaiI ACGT 4 cut(s) 1413, 1693, 1750, 1894
TaqI TCGA 6 cut(s) 303, 860, 1300, 1612, 1819, 2212
TaqII GACCGA 1 cut(s) 127
TatI WGTACW 1 cut(s) 1391
TfiI GAWTC 3 cut(s) 159, 731, 1061
Tru1I TTAA 6 cut(s) 506, 1188, 1268, 1344, 1350, 2144
Tru9I TTAA 6 cut(s) 506, 1188, 1268, 1344, 1350, 2144
TscAI CASTG 2 cut(s) 1369, 1423
TseFI GTSAC 2 cut(s) 553, 1645
Tsp45I GTSAC 2 cut(s) 553, 1645
TspGWI ACGGA 1 cut(s) 1530
TspRI CASTG 2 cut(s) 1369, 1423
Van91I CCANNNNNTGG 2 cut(s) 49, 1948
VpaK11BI GGWCC 4 cut(s) 153, 520, 1332, 2256
XagI CCTNNNNNAGG 1 cut(s) 74
XapI RAATTY 3 cut(s) 826, 864, 961
XceI RCATGY 1 cut(s) 1307
XmiI GTMKAC 3 cut(s) 221, 1407, 1497
XmnI GAANNNNTTC 3 cut(s) 1051, 1908, 2208
XspI CTAG 6 cut(s) 975, 984, 1733, 1844, 2105, 2175
ZrmI AGTACT 1 cut(s) 1393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.