FvH4_4g19753

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000660
Physical Location & Seq
Forward (+)
23313174 .. 23316323
3150 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g19753.t1

Sequence Viewer

Length: 756 bp
ATGGTTTGCTTTGTTGGATTTCATCCTGATATCCTTTTGCCATATGACTTTCCTCTGCAGGTTCATGTGGAACTGTGTACCATGGATGTTGCTGTTAAGGTATTCTTCAAGCAAGAATATCCAGATGATGTACTTCTTTCTTTTAGACAAGAGGCTTCAACATCCAAACATTCTGCTTTTCATGGGAGCTGTGACTTCACCTCAGCATCTTTGCATTGTCACAGAGTTCCTCCCACGAAGAACAAGTCAAAGCTAGACTGGAGACGACGTGTTCAAATGGCTTTGGACATAGCACGAGGCATGAATTATCTTCATCATTTCAGACCCCCTATTATTCATCGAGATTTGAAGTCTTCAAATCTCTTAGTTGATAAGAACTGGACTGTCAAGGTTGGTGATTTTGGTCTTTCACGTCTTAAGCATGAGACGTATCTCTTTACCAAGACTGGGAAGGGGACGCCTCAATGGATGGCACCAGAAGTTCTGTGTAATGAATTCTCAGATGAAAAGTCTGATGTATACAGCTACGGGGTGATTGGAGCTGTAAGTTTCAGGAACCAAAGGCTAGAGATCCCAATAGATGTGGATCCACAATGGGCTTCTATGATTGAGAGCTGTTGGCACAGGTGTGATCCAGCTTGCCGGCCAACATTTCATGAATTGCTGGAAAAACTTAAAGCGTTGCAGAGACAGCGTGCTCTCCAATTCAAAGCAGCACGTTCTGCCTCCAGTTATAGCTCCCAAAACGAAACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS PAS_9 Pkinase_Tyr

Protein Analysis

252

Amino Acids

28.96

Weight (kDa)

8.58

Isoelectric Point (pI)

43.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 80 - 179 1.5e-27 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 83 - 181 2e-24 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 49
AccB1I GGYRCC 1 cut(s) 472
AccI GTMKAC 1 cut(s) 519
AclWI GGATC 4 cut(s) 565, 581, 594, 626
AcoI YGGCCR 1 cut(s) 644
AcsI RAATTY 1 cut(s) 494
AcyI GRCGYC 1 cut(s) 458
AfaI GTAC 2 cut(s) 79, 132
AfiI CCNNNNNNNGG 1 cut(s) 447
AflII CTTAAG 1 cut(s) 416
AflIII ACRYGT 1 cut(s) 268
AgsI TTSAA 6 cut(s) 109, 159, 275, 349, 357, 709
AjiI CACGTC 2 cut(s) 269, 413
AleI CACNNNNGTG 1 cut(s) 627
AluBI AGCT 7 cut(s) 189, 253, 525, 542, 615, 638, 738
AluI AGCT 7 cut(s) 189, 253, 525, 542, 615, 638, 738
Alw21I GWGCWC 1 cut(s) 700
Alw26I GTCTC 3 cut(s) 256, 419, 682
AlwI GGATC 4 cut(s) 565, 581, 594, 626
AoxI GGCC 1 cut(s) 644
ApeKI GCWGC 1 cut(s) 713
ApoI RAATTY 1 cut(s) 494
ArsI GACNNNNNNTTYG 2 cut(s) 230, 262
AsuHPI GGTGA 3 cut(s) 190, 407, 544
BamHI GGATCC 1 cut(s) 586
BanI GGYRCC 1 cut(s) 472
BauI CACGAG 1 cut(s) 294
BbsI GAAGAC 1 cut(s) 345
Bbv12I GWGCWC 1 cut(s) 700
BbvCI CCTCAGC 1 cut(s) 202
BbvI GCAGC 1 cut(s) 725
BccI CCATC 1 cut(s) 463
BcoDI GTCTC 3 cut(s) 256, 419, 682
BfaI CTAG 2 cut(s) 254, 566
BfmI CTRYAG 1 cut(s) 56
BfrI CTTAAG 1 cut(s) 416
BfuAI ACCTGC 1 cut(s) 49
BisI GCNGC 1 cut(s) 714
BlsI GCNGC 1 cut(s) 715
BmgBI CACGTC 2 cut(s) 269, 413
BmiI GGNNCC 3 cut(s) 474, 557, 588
BmrI ACTGGG 1 cut(s) 456
BmsI GCATC 1 cut(s) 215
BmuI ACTGGG 1 cut(s) 456
BpiI GAAGAC 1 cut(s) 345
BpmI CTGGAG 2 cut(s) 280, 712
Bpu10I CCTNAGC 1 cut(s) 202
BsaBI GATNNNNATC 1 cut(s) 585
BsaHI GRCGYC 1 cut(s) 458
BsaJI CCNNGG 1 cut(s) 81
BsaXI ACNNNNNCTCC 2 cut(s) 253, 283
Bsc4I CCNNNNNNNGG 1 cut(s) 447
Bse118I RCCGGY 1 cut(s) 642
Bse1I ACTGG 4 cut(s) 263, 383, 451, 729
Bse8I GATNNNNATC 1 cut(s) 585
BseDI CCNNGG 1 cut(s) 81
BseGI GGATG 4 cut(s) 22, 91, 161, 474
BseJI GATNNNNATC 1 cut(s) 585
BseLI CCNNNNNNNGG 1 cut(s) 447
BseMII CTCAG 2 cut(s) 216, 513
BseNI ACTGG 4 cut(s) 263, 383, 451, 729
BseXI GCAGC 1 cut(s) 725
BshFI GGCC 1 cut(s) 646
BshNI GGYRCC 1 cut(s) 472
BsiHKAI GWGCWC 1 cut(s) 700
BsiSI CCGG 1 cut(s) 643
BslFI GGGAC 1 cut(s) 469
BslI CCNNNNNNNGG 1 cut(s) 447
BsmAI GTCTC 3 cut(s) 256, 419, 682
BsmBI CGTCTC 2 cut(s) 256, 419
BsmFI GGGAC 1 cut(s) 469
BsnI GGCC 1 cut(s) 646
Bsp1286I GDGCHC 1 cut(s) 700
Bsp143I GATC 3 cut(s) 570, 586, 631
Bsp19I CCATGG 1 cut(s) 81
BspANI GGCC 1 cut(s) 646
BspCNI CTCAG 2 cut(s) 215, 512
BspHI TCATGA 1 cut(s) 655
BspLI GGNNCC 3 cut(s) 474, 557, 588
BspMAI CTGCAG 1 cut(s) 60
BspMI ACCTGC 1 cut(s) 49
BspPI GGATC 4 cut(s) 565, 581, 594, 626
BspT107I GGYRCC 1 cut(s) 472
BspTI CTTAAG 1 cut(s) 416
BsrFI RCCGGY 1 cut(s) 642
BsrI ACTGG 4 cut(s) 263, 383, 451, 729
BssAI RCCGGY 1 cut(s) 642
BssECI CCNNGG 1 cut(s) 81
BssMI GATC 3 cut(s) 570, 586, 631
BssNAI GTATAC 1 cut(s) 520
BssNI GRCGYC 1 cut(s) 458
BssSI CACGAG 1 cut(s) 294
BssT1I CCWWGG 1 cut(s) 81
Bst1107I GTATAC 1 cut(s) 520
Bst2BI CACGAG 1 cut(s) 294
Bst4CI ACNGT 2 cut(s) 75, 385
BstACI GRCGYC 1 cut(s) 458
BstAFI CTTAAG 1 cut(s) 416
BstAPI GCANNNNNTGC 1 cut(s) 722
BstC8I GCNNGC 3 cut(s) 640, 644, 696
BstDEI CTNAG 3 cut(s) 202, 364, 499
BstDSI CCRYGG 1 cut(s) 81
BstF5I GGATG 4 cut(s) 22, 91, 161, 474
BstKTI GATC 3 cut(s) 573, 589, 634
BstMAI GTCTC 3 cut(s) 256, 419, 682
BstMBI GATC 3 cut(s) 570, 586, 631
BstMWI GCNNNNNNNGC 2 cut(s) 691, 722
BstSFI CTRYAG 1 cut(s) 56
BstV1I GCAGC 1 cut(s) 725
BstV2I GAAGAC 1 cut(s) 345
BstX2I RGATCY 2 cut(s) 570, 586
BstYI RGATCY 2 cut(s) 570, 586
BstZ17I GTATAC 1 cut(s) 520
BsuRI GGCC 1 cut(s) 646
BtgI CCRYGG 1 cut(s) 81
BtrI CACGTC 2 cut(s) 269, 413
BtsCI GGATG 4 cut(s) 22, 91, 161, 474
BveI ACCTGC 1 cut(s) 49
Cac8I GCNNGC 3 cut(s) 640, 644, 696
CciI TCATGA 1 cut(s) 655
Cfr10I RCCGGY 1 cut(s) 642
CseI GACGC 1 cut(s) 466
Csp6I GTAC 2 cut(s) 78, 131
CspCI CAANNNNNGTGG 2 cut(s) 564, 599
CviAII CATG 6 cut(s) 65, 82, 182, 301, 422, 656
CviQI GTAC 2 cut(s) 78, 131
DdeI CTNAG 3 cut(s) 202, 364, 499
DpnI GATC 3 cut(s) 572, 588, 633
DpnII GATC 3 cut(s) 570, 586, 631
EaeI YGGCCR 1 cut(s) 644
Eco130I CCWWGG 1 cut(s) 81
Eco32I GATATC 1 cut(s) 31
EcoRI GAATTC 1 cut(s) 494
EcoRV GATATC 1 cut(s) 31
EcoT14I CCWWGG 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 81
Esp3I CGTCTC 2 cut(s) 256, 419
FaeI CATG 6 cut(s) 68, 85, 185, 304, 425, 659
FalI AAGNNNNNCTT 2 cut(s) 89, 121
FaqI GGGAC 1 cut(s) 469
FatI CATG 6 cut(s) 64, 81, 181, 300, 421, 655
FauNDI CATATG 1 cut(s) 43
FblI GTMKAC 1 cut(s) 519
Fnu4HI GCNGC 1 cut(s) 714
FokI GGATG 4 cut(s) 9, 98, 148, 481
Fsp4HI GCNGC 1 cut(s) 714
FspBI CTAG 2 cut(s) 254, 566
GluI GCNGC 1 cut(s) 714
GsuI CTGGAG 2 cut(s) 280, 712
HaeIII GGCC 1 cut(s) 646
HapII CCGG 1 cut(s) 643
HgaI GACGC 1 cut(s) 466
Hin1I GRCGYC 1 cut(s) 458
Hin1II CATG 6 cut(s) 68, 85, 185, 304, 425, 659
HpaII CCGG 1 cut(s) 643
HphI GGTGA 3 cut(s) 190, 407, 544
Hpy166II GTNNAC 2 cut(s) 78, 520
Hpy188I TCNGA 3 cut(s) 323, 502, 514
Hpy188III TCNNGA 5 cut(s) 26, 122, 341, 553, 656
Hpy8I GTNNAC 2 cut(s) 78, 520
Hpy99I CGWCG 1 cut(s) 270
HpyAV CCTTC 1 cut(s) 445
HpyCH4III ACNGT 2 cut(s) 75, 385
HpyCH4IV ACGT 5 cut(s) 268, 412, 428, 718, 752
HpyCH4V TGCA 3 cut(s) 58, 214, 685
HpyF10VI GCNNNNNNNGC 2 cut(s) 691, 722
HpyF3I CTNAG 3 cut(s) 202, 364, 499
HpySE526I ACGT 5 cut(s) 268, 412, 428, 718, 752
Hsp92I GRCGYC 1 cut(s) 458
Hsp92II CATG 6 cut(s) 68, 85, 185, 304, 425, 659
KroI GCCGGC 1 cut(s) 642
KroNI GCCGGC 1 cut(s) 644
Kzo9I GATC 3 cut(s) 570, 586, 631
LmnI GCTCC 3 cut(s) 186, 539, 743
Lsp1109I GCAGC 1 cut(s) 725
LweI GCATC 1 cut(s) 215
MaeI CTAG 2 cut(s) 254, 566
MaeII ACGT 5 cut(s) 268, 412, 428, 718, 752
MaeIII GTNAC 2 cut(s) 191, 218
MalI GATC 3 cut(s) 572, 588, 633
MboI GATC 3 cut(s) 570, 586, 631
MboII GAAGA 4 cut(s) 97, 250, 302, 345
MflI RGATCY 2 cut(s) 570, 586
MhlI GDGCHC 1 cut(s) 700
MluCI AATT 4 cut(s) 304, 494, 659, 704
MnlI CCTC 7 cut(s) 63, 145, 211, 240, 290, 471, 736
MroNI GCCGGC 1 cut(s) 642
MseI TTAA 3 cut(s) 96, 417, 675
MslI CAYNNNNRTG 1 cut(s) 627
MspCI CTTAAG 1 cut(s) 416
MspI CCGG 1 cut(s) 643
MwoI GCNNNNNNNGC 2 cut(s) 691, 722
NaeI GCCGGC 1 cut(s) 644
NcoI CCATGG 1 cut(s) 81
NdeI CATATG 1 cut(s) 43
NdeII GATC 3 cut(s) 570, 586, 631
NgoMIV GCCGGC 1 cut(s) 642
NlaIII CATG 6 cut(s) 68, 85, 185, 304, 425, 659
NlaIV GGNNCC 3 cut(s) 474, 557, 588
NmuCI GTSAC 2 cut(s) 191, 218
OliI CACNNNNGTG 1 cut(s) 627
PagI TCATGA 1 cut(s) 655
PdiI GCCGGC 1 cut(s) 644
PkrI GCNGC 1 cut(s) 715
PspN4I GGNNCC 3 cut(s) 474, 557, 588
PstI CTGCAG 1 cut(s) 60
PsuI RGATCY 2 cut(s) 570, 586
RsaI GTAC 2 cut(s) 79, 132
RsaNI GTAC 2 cut(s) 78, 131
RseI CAYNNNNRTG 1 cut(s) 627
SaqAI TTAA 3 cut(s) 96, 417, 675
SatI GCNGC 1 cut(s) 714
Sau3AI GATC 3 cut(s) 570, 586, 631
SduI GDGCHC 1 cut(s) 700
SfaNI GCATC 1 cut(s) 215
SfcI CTRYAG 1 cut(s) 56
SmiMI CAYNNNNRTG 1 cut(s) 627
SmlI CTYRAG 1 cut(s) 416
SmoI CTYRAG 1 cut(s) 416
Sse9I AATT 4 cut(s) 304, 494, 659, 704
SspMI CTAG 2 cut(s) 254, 566
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 2 cut(s) 75, 385
TaiI ACGT 5 cut(s) 271, 415, 431, 721, 755
TaqI TCGA 1 cut(s) 340
TasI AATT 4 cut(s) 304, 494, 659, 704
TatI WGTACW 1 cut(s) 130
Tru1I TTAA 3 cut(s) 96, 417, 675
Tru9I TTAA 3 cut(s) 96, 417, 675
TseFI GTSAC 2 cut(s) 191, 218
TseI GCWGC 1 cut(s) 713
Tsp45I GTSAC 2 cut(s) 191, 218
Vha464I CTTAAG 1 cut(s) 416
XapI RAATTY 1 cut(s) 494
XmiI GTMKAC 1 cut(s) 519
XspI CTAG 2 cut(s) 254, 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.