RchiOBHm_Chr4g0425321

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000660
Physical Location & Seq
Forward (+)
50922141 .. 50927232
5092 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ39448

Sequence Viewer

Length: 2265 bp
ATGGATGATACACCACCAGCAAAGGAGCTTCTGAAGAAGCTTCAAGTGCTGGAAGAGGGTCACGCCTACCTCAAGCAAGAGCTCTCGGAGTATTCCTATGCCAAACTGGAGAACCAAAGGGCTCTCTCAAAAAGGCGCTCAAGATTCTCTTCCAAAAGAAGAGCCGGTGGTGGTGGCGGTGCAAGTTGGCGGCCGCCGAGTCTCAACTTCAGTGACAGGCAGTATCTCAATATATTGCAGTCAATAGGGCAGTCTGTGTACATATTTGATCTCAGTTGTCGTATAATTTACTGGAGCAAGACTGCTGGAAATCTATATGGTTATTCTGTGGCAGAGGCCCTTGGACAGAATCCCCTTGAGCTCTTAGCAGATCCCCAGGCCTTTGCTGTAGGATATAGTATAGTTCAACGTCTCATCAAGGAGGAGAGCTGGACCGGGCAGTTCCCTGTCAAGAACAAAATGGGGGAAATTTTTTCAGCCATGGCCACCAATACTCCATTTTATGATGATGATGGCACTTTCATTGGGGTTATATGTGTTTCATGGGATTTGCGGCATTTTCAAGAAACGAGGGTTCCACTGTTATGTTTAAAACCAGACTCAAGCTTCGGCCGTTGCCCTACCACTAGTGCGGCAACTCAAGTTGGTGTTGATCCTCAGCAACCTCTGCAAAATGAACTTGCATCAATAATAACAAATTTGGCGTCCAAGGTCTGCAACAAATTCAAGTCAAGAGTTAAGGATGGAAAAGAAAACTTGGATGACAAAGCTGGGAGTGGAGATAGTGGCTGGTGTGATCATGGTTCAGTTTCTCATCTCTCTGACCATTTGGAGGATGGGTGTTCAATTGGGGCAAGCAAACCGAGAGGAGGTATACATCCATCTCCTTTTGGTATGTTTGCCAAAATTAAAGACAAGTTTCCAGGTAAACCCTCCGGAGAATATGATGATCAGAGTGAAGGAAAACGCCCAATGCATAAGATATTAACCTCAAAGGCAGAAGCATGGATTGGTAAGAAGGGACTATCATGGCCATGGAAAGAAAATGAGAAAGAAGGGTCGGTGACTAGGAGCACTCATTTTGTTTGGCCCTGGTTGCATAATGATCAAGGAAAAGATTCAATACCTCAGGAGGGTAATTGTGCTTTAAAACCAGAAAATCAGGTGAATGAAAGTAATCAACCTGCAACTAATGAAGCTTCAGGAGCCTGGACCTCTTTGTTTAATGTTAACTGCACAAGACGTGGTAGCAGTTCTGGAAGTACCAACAGTTCTGCCAAAAAGGAGGATGTGGATATTGATTGCTTGGACTATGAAGTCTTATGGGAAGACATGATAATTAGAGAGAGGATTGGGCAAGGTTCATGTGGAACTGTATACCATGGTGTGTTGGATGAATCAGATATTGCTGTTAAGGTATTTTTGAAGCAAGAATATCCAGATGATGTGCTTCTGTCTTTTAGACAAGAGGTATCTCTTATGAAAAGGCTTCGACATCCAAACGTGCTGCTTTTCATGGGAGCGGTGACTTCACCTCAGCGTCTTTGCATTGTAACAGAGTTCCTCCCACGTGGAAGTTTGTTTCAATTACTACAGAAGAACAAGTCAAAATTAGACTGGAGACGACGTGTTCAAATGGCTTTAGATATAGCTCTTGAGGCATTTTATATCAGACTATCTTCTGGATTGCATCAAATTGTGTTCATCTGGTGTTTGAATAGAGTCTTGGCACGAGGCATGAATTATCTTCATCATTTCAGCCCCCCTATTATTCATCGAGATCTGAAGTCTTCAAATCTCCTAGTTGATAAGAACTGGACTGTCAAGGTTGGTGACTTTGGTCTTTCACGTCTTAAGCATGAAGCATTTCTCTATACCAAGACCGGAAAGGGGACGCCTCAATGGATGGCACCAGAAGTTCTGTGTAATGAATCCTCAGATGAGAAGTCTGATGTATTCAGCTACGGGGTGATATTGTGGGAGCTCGCTACTGAGAAGATTCCTTGGGATGATCTGAATGCAATGCAGGTGATTGGAGCTGTAGGGTTCAGGAACCAAAGGCTAGAGATCCCAAAGGATGTGGATCCACAATGGGCTTCTATGATTGAGAGATGTTGGCACAGTGATCCAGCTTGCCGGCCAACATTTCATGAATTGCTGGAAGAACTTAAAGCGTTGCAGAGACGGCGTGCTCTCCAGTTCAAAGCAGCGCGTTCTGCCTCTAGTAGCACCCAAAACGAATTGCAGACCAGCCTGAAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS PAS_9 Pkinase_Tyr

Protein Analysis

754

Amino Acids

84.9

Weight (kDa)

8.02

Isoelectric Point (pI)

44.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS PF00989 74 - 180 1.8e-08 PAS fold
PAS_4 PF08448 80 - 185 6.9e-06 PAS fold
PAS_9 PF13426 87 - 183 2.3e-08 PAS domain
PK_Tyr_Ser-Thr PF07714 447 - 564 3.5e-21 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 448 - 721 6.7e-49 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 575 - 723 2.9e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2017
AasI GACNNNNNNGTC 1 cut(s) 1316
Acc36I ACCTGC 2 cut(s) 1192, 2017
AccB1I GGYRCC 1 cut(s) 1909
AccBSI CCGCTC 1 cut(s) 1523
AccI GTMKAC 2 cut(s) 874, 1377
AccII CGCG 1 cut(s) 2212
AccIII TCCGGA 1 cut(s) 935
AciI CCGC 6 cut(s) 177, 190, 194, 553, 632, 1523
AclWI GGATC 6 cut(s) 365, 647, 2062, 2078, 2091, 2120
AcoI YGGCCR 5 cut(s) 191, 483, 610, 1031, 2138
AcsI RAATTY 3 cut(s) 468, 697, 722
AcuI CTGAAG 4 cut(s) 53, 193, 1185, 1805
AcvI CACGTG 1 cut(s) 1571
AcyI GRCGYC 2 cut(s) 704, 1895
AfaI GTAC 2 cut(s) 260, 1264
AfiI CCNNNNNNNGG 4 cut(s) 832, 869, 1133, 1890
AflII CTTAAG 1 cut(s) 1853
AflIII ACRYGT 1 cut(s) 1627
AhlI ACTAGT 1 cut(s) 626
AjiI CACGTC 3 cut(s) 1244, 1628, 1850
AjnI CCWGG 4 cut(s) 375, 922, 1091, 1208
AjuI GAANNNNNNNTTGG 4 cut(s) 993, 1025, 1709, 1741
Alw21I GWGCWC 5 cut(s) 84, 363, 1076, 1986, 2194
Alw26I GTCTC 4 cut(s) 206, 416, 1615, 2176
AlwI GGATC 6 cut(s) 365, 647, 2062, 2078, 2091, 2120
Aor13HI TCCGGA 1 cut(s) 935
AoxI GGCC 8 cut(s) 191, 336, 378, 483, 610, 1031, 1088, 2138
ApeKI GCWGC 2 cut(s) 1507, 2207
ApoI RAATTY 3 cut(s) 468, 697, 722
ArsI GACNNNNNNTTYG 4 cut(s) 590, 622, 689, 721
Asp700I GAANNNNTTC 2 cut(s) 1117, 1866
AspLEI GCGC 2 cut(s) 138, 2212
AspS9I GGNCC 4 cut(s) 337, 432, 1089, 1212
AsuC2I CCSGG 1 cut(s) 436
AsuHPI GGTGA 7 cut(s) 1075, 1177, 1524, 1537, 1844, 1981, 2041
AvaII GGWCC 2 cut(s) 432, 1212
AxyI CCTNAGG 1 cut(s) 1128
BalI TGGCCA 2 cut(s) 485, 1033
BamHI GGATCC 1 cut(s) 2083
BanI GGYRCC 1 cut(s) 1909
BanII GRGCYC 4 cut(s) 84, 124, 363, 1986
BauI CACGAG 1 cut(s) 1731
BbrPI CACGTG 1 cut(s) 1571
BbsI GAAGAC 2 cut(s) 1335, 1782
Bbv12I GWGCWC 5 cut(s) 84, 363, 1076, 1986, 2194
BbvCI CCTCAGC 2 cut(s) 657, 1536
BbvI GCAGC 2 cut(s) 1494, 2219
BccI CCATC 5 cut(s) 506, 737, 830, 889, 1900
BceAI ACGGC 2 cut(s) 597, 2201
BciT130I CCWGG 4 cut(s) 377, 924, 1093, 1210
BclI TGATCA 3 cut(s) 796, 949, 1105
BcnI CCSGG 1 cut(s) 436
BcoDI GTCTC 4 cut(s) 206, 416, 1615, 2176
BcuI ACTAGT 1 cut(s) 626
BfaI CTAG 5 cut(s) 627, 1068, 1802, 2063, 2223
BfmI CTRYAG 3 cut(s) 387, 1592, 2040
BfoI RGCGCY 1 cut(s) 139
BfrI CTTAAG 1 cut(s) 1853
BfuAI ACCTGC 2 cut(s) 1192, 2017
BglII AGATCT 1 cut(s) 1780
BisI GCNGC 6 cut(s) 191, 194, 554, 633, 1508, 2208
BlsI GCNGC 6 cut(s) 192, 195, 555, 634, 1509, 2209
Bme1390I CCNGG 5 cut(s) 377, 436, 924, 1093, 1210
Bme18I GGWCC 2 cut(s) 432, 1212
BmgBI CACGTC 3 cut(s) 1244, 1628, 1850
BmgT120I GGNCC 4 cut(s) 337, 432, 1089, 1212
BmiI GGNNCC 5 cut(s) 576, 1207, 1911, 2054, 2085
BmrFI CCNGG 5 cut(s) 377, 436, 924, 1093, 1210
BmsI GCATC 2 cut(s) 692, 1699
BoxI GACNNNNGTC 1 cut(s) 1839
BpiI GAAGAC 2 cut(s) 1335, 1782
BpmI CTGGAG 4 cut(s) 128, 313, 1639, 2180
Bpu10I CCTNAGC 2 cut(s) 657, 1536
BpuEI CTTGAG 6 cut(s) 56, 124, 377, 586, 624, 1676
BpuMI CCSGG 1 cut(s) 436
BsaAI YACGTR 1 cut(s) 1571
BsaBI GATNNNNATC 1 cut(s) 2082
BsaHI GRCGYC 2 cut(s) 704, 1895
BsaJI CCNNGG 8 cut(s) 340, 375, 480, 708, 1034, 1091, 1381, 2003
BsaWI WCCGGW 2 cut(s) 935, 1883
BsaXI ACNNNNNCTCC 6 cut(s) 478, 508, 824, 854, 1612, 1642
Bsc4I CCNNNNNNNGG 4 cut(s) 832, 869, 1133, 1890
Bse118I RCCGGY 2 cut(s) 164, 2136
Bse1I ACTGG 5 cut(s) 111, 296, 1622, 1820, 2197
Bse21I CCTNAGG 1 cut(s) 1128
Bse3DI GCAATG 1 cut(s) 2028
Bse8I GATNNNNATC 1 cut(s) 2082
BseAI TCCGGA 1 cut(s) 935
BseBI CCWGG 4 cut(s) 377, 924, 1093, 1210
BseDI CCNNGG 8 cut(s) 340, 375, 480, 708, 1034, 1091, 1381, 2003
BseJI GATNNNNATC 1 cut(s) 2082
BseLI CCNNNNNNNGG 4 cut(s) 832, 869, 1133, 1890
BseMI GCAATG 1 cut(s) 2028
BseMII CTCAG 6 cut(s) 286, 671, 1142, 1550, 1950, 1983
BseNI ACTGG 5 cut(s) 111, 296, 1622, 1820, 2197
BseRI GAGGAG 2 cut(s) 437, 882
BseX3I CGGCCG 2 cut(s) 191, 610
BseXI GCAGC 2 cut(s) 1494, 2219
BseYI CCCAGC 1 cut(s) 770
BsgI GTGCAG 1 cut(s) 1219
Bsh1236I CGCG 1 cut(s) 2212
Bsh1285I CGRYCG 2 cut(s) 194, 613
BshFI GGCC 8 cut(s) 193, 338, 380, 485, 612, 1033, 1090, 2140
BshNI GGYRCC 1 cut(s) 1909
BsiEI CGRYCG 2 cut(s) 194, 613
BsiHKAI GWGCWC 5 cut(s) 84, 363, 1076, 1986, 2194
BsiSI CCGG 5 cut(s) 165, 435, 936, 1884, 2137
BslFI GGGAC 2 cut(s) 1035, 1906
BslI CCNNNNNNNGG 4 cut(s) 832, 869, 1133, 1890
BsmAI GTCTC 4 cut(s) 206, 416, 1615, 2176
BsmBI CGTCTC 3 cut(s) 416, 1615, 2176
BsmFI GGGAC 2 cut(s) 1035, 1906
BsmI GAATGC 1 cut(s) 2023
BsnI GGCC 8 cut(s) 193, 338, 380, 485, 612, 1033, 1090, 2140
Bsp1286I GDGCHC 6 cut(s) 84, 124, 363, 1076, 1986, 2194
Bsp13I TCCGGA 1 cut(s) 935
Bsp1407I TGTACA 1 cut(s) 258
Bsp19I CCATGG 3 cut(s) 480, 1034, 1381
BspACI CCGC 6 cut(s) 177, 190, 194, 553, 632, 1523
BspANI GGCC 8 cut(s) 193, 338, 380, 485, 612, 1033, 1090, 2140
BspCNI CTCAG 6 cut(s) 285, 670, 1141, 1549, 1949, 1984
BspEI TCCGGA 1 cut(s) 935
BspFNI CGCG 1 cut(s) 2212
BspHI TCATGA 1 cut(s) 2149
BspLI GGNNCC 5 cut(s) 576, 1207, 1911, 2054, 2085
BspMI ACCTGC 2 cut(s) 1192, 2017
BspPI GGATC 6 cut(s) 365, 647, 2062, 2078, 2091, 2120
BspQI GCTCTTC 1 cut(s) 154
BspT107I GGYRCC 1 cut(s) 1909
BspTI CTTAAG 1 cut(s) 1853
BsrBI CCGCTC 1 cut(s) 1523
BsrDI GCAATG 1 cut(s) 2028
BsrFI RCCGGY 2 cut(s) 164, 2136
BsrGI TGTACA 1 cut(s) 258
BsrI ACTGG 5 cut(s) 111, 296, 1622, 1820, 2197
BssAI RCCGGY 2 cut(s) 164, 2136
BssECI CCNNGG 8 cut(s) 340, 375, 480, 708, 1034, 1091, 1381, 2003
BssNAI GTATAC 2 cut(s) 875, 1378
BssNI GRCGYC 2 cut(s) 704, 1895
BssSI CACGAG 1 cut(s) 1731
BssT1I CCWWGG 6 cut(s) 340, 480, 708, 1034, 1381, 2003
Bst1107I GTATAC 2 cut(s) 875, 1378
Bst2BI CACGAG 1 cut(s) 1731
Bst2UI CCWGG 4 cut(s) 377, 924, 1093, 1210
Bst4CI ACNGT 5 cut(s) 582, 1271, 1375, 1822, 2123
Bst6I CTCTTC 3 cut(s) 48, 154, 154
BstACI GRCGYC 2 cut(s) 704, 1895
BstAFI CTTAAG 1 cut(s) 1853
BstAPI GCANNNNNTGC 1 cut(s) 667
BstAUI TGTACA 1 cut(s) 258
BstBAI YACGTR 1 cut(s) 1571
BstC8I GCNNGC 5 cut(s) 856, 1986, 2134, 2138, 2190
BstDEI CTNAG 7 cut(s) 272, 364, 657, 1128, 1536, 1936, 1992
BstDSI CCRYGG 3 cut(s) 480, 1034, 1381
BstFNI CGCG 1 cut(s) 2212
BstH2I RGCGCY 1 cut(s) 139
BstHHI GCGC 2 cut(s) 138, 2212
BstMAI GTCTC 4 cut(s) 206, 416, 1615, 2176
BstMCI CGRYCG 2 cut(s) 194, 613
BstMWI GCNNNNNNNGC 7 cut(s) 46, 667, 1096, 1205, 1658, 2185, 2216
BstNI CCWGG 4 cut(s) 377, 924, 1093, 1210
BstPAI GACNNNNGTC 1 cut(s) 1839
BstSCI CCNGG 5 cut(s) 375, 434, 922, 1091, 1208
BstSFI CTRYAG 3 cut(s) 387, 1592, 2040
BstUI CGCG 1 cut(s) 2212
BstV1I GCAGC 2 cut(s) 1494, 2219
BstV2I GAAGAC 2 cut(s) 1335, 1782
BstX2I RGATCY 4 cut(s) 370, 1780, 2067, 2083
BstYI RGATCY 4 cut(s) 370, 1780, 2067, 2083
BstZ17I GTATAC 2 cut(s) 875, 1378
BstZI CGGCCG 2 cut(s) 191, 610
Bsu36I CCTNAGG 1 cut(s) 1128
BsuRI GGCC 8 cut(s) 193, 338, 380, 485, 612, 1033, 1090, 2140
BtgI CCRYGG 3 cut(s) 480, 1034, 1381
BtrI CACGTC 3 cut(s) 1244, 1628, 1850
BtsIMutI CAGTG 3 cut(s) 217, 578, 2128
BveI ACCTGC 2 cut(s) 1192, 2017
Cac8I GCNNGC 5 cut(s) 856, 1986, 2134, 2138, 2190
CciI TCATGA 1 cut(s) 2149
CciNI GCGGCCGC 1 cut(s) 191
CfoI GCGC 2 cut(s) 138, 2212
Cfr10I RCCGGY 2 cut(s) 164, 2136
Cfr13I GGNCC 4 cut(s) 337, 432, 1089, 1212
CseI GACGC 3 cut(s) 693, 1529, 1903
Csp6I GTAC 2 cut(s) 259, 1263
CspCI CAANNNNNGTGG 2 cut(s) 2061, 2096
CviQI GTAC 2 cut(s) 259, 1263
DdeI CTNAG 7 cut(s) 272, 364, 657, 1128, 1536, 1936, 1992
DraI TTTAAA 2 cut(s) 591, 1149
DrdI GACNNNNNNGTC 1 cut(s) 1316
DseDI GACNNNNNNGTC 1 cut(s) 1316
EaeI YGGCCR 5 cut(s) 191, 483, 610, 1031, 2138
EagI CGGCCG 2 cut(s) 191, 610
Eam1104I CTCTTC 3 cut(s) 48, 154, 154
EarI CTCTTC 3 cut(s) 48, 154, 154
Ecl136II GAGCTC 3 cut(s) 82, 361, 1984
EclXI CGGCCG 2 cut(s) 191, 610
Eco130I CCWWGG 6 cut(s) 340, 480, 708, 1034, 1381, 2003
Eco147I AGGCCT 1 cut(s) 380
Eco24I GRGCYC 4 cut(s) 84, 124, 363, 1986
Eco47I GGWCC 2 cut(s) 432, 1212
Eco52I CGGCCG 2 cut(s) 191, 610
Eco53kI GAGCTC 3 cut(s) 82, 361, 1984
Eco57I CTGAAG 4 cut(s) 53, 193, 1185, 1805
Eco72I CACGTG 1 cut(s) 1571
Eco81I CCTNAGG 1 cut(s) 1128
EcoICRI GAGCTC 3 cut(s) 82, 361, 1984
EcoO109I RGGNCCY 1 cut(s) 337
EcoRII CCWGG 4 cut(s) 375, 922, 1091, 1208
EcoT14I CCWWGG 6 cut(s) 340, 480, 708, 1034, 1381, 2003
EcoT22I ATGCAT 1 cut(s) 978
EcoT38I GRGCYC 4 cut(s) 84, 124, 363, 1986
ErhI CCWWGG 6 cut(s) 340, 480, 708, 1034, 1381, 2003
Esp3I CGTCTC 3 cut(s) 416, 1615, 2176
FalI AAGNNNNNCTT 2 cut(s) 133, 165
FaqI GGGAC 2 cut(s) 1035, 1906
FbaI TGATCA 3 cut(s) 796, 949, 1105
FblI GTMKAC 2 cut(s) 874, 1377
Fnu4HI GCNGC 6 cut(s) 191, 194, 554, 633, 1508, 2208
FriOI GRGCYC 4 cut(s) 84, 124, 363, 1986
Fsp4HI GCNGC 6 cut(s) 191, 194, 554, 633, 1508, 2208
FspBI CTAG 5 cut(s) 627, 1068, 1802, 2063, 2223
GlaI GCGC 2 cut(s) 137, 2211
GluI GCNGC 6 cut(s) 191, 194, 554, 633, 1508, 2208
GsaI CCCAGC 1 cut(s) 774
GsuI CTGGAG 4 cut(s) 128, 313, 1639, 2180
HaeII RGCGCY 1 cut(s) 139
HaeIII GGCC 8 cut(s) 193, 338, 380, 485, 612, 1033, 1090, 2140
HapII CCGG 5 cut(s) 165, 435, 936, 1884, 2137
HgaI GACGC 3 cut(s) 693, 1529, 1903
HhaI GCGC 2 cut(s) 138, 2212
Hin1I GRCGYC 2 cut(s) 704, 1895
Hin6I GCGC 2 cut(s) 136, 2210
HinP1I GCGC 2 cut(s) 136, 2210
HincII GTYRAC 1 cut(s) 1231
HindII GTYRAC 1 cut(s) 1231
HindIII AAGCTT 3 cut(s) 38, 604, 1197
HinfI GANTC 9 cut(s) 144, 199, 349, 599, 1118, 1397, 1722, 1931, 1999
HpaI GTTAAC 1 cut(s) 1231
HpaII CCGG 5 cut(s) 165, 435, 936, 1884, 2137
HphI GGTGA 7 cut(s) 1075, 1177, 1524, 1537, 1844, 1981, 2041
Hpy166II GTNNAC 5 cut(s) 259, 875, 929, 1231, 1378
Hpy8I GTNNAC 5 cut(s) 259, 875, 929, 1231, 1378
Hpy99I CGWCG 1 cut(s) 1629
HpyAV CCTTC 3 cut(s) 953, 1012, 1049
HpyCH4III ACNGT 5 cut(s) 582, 1271, 1375, 1822, 2123
HpyCH4IV ACGT 6 cut(s) 409, 1243, 1503, 1570, 1627, 1849
HpyF10VI GCNNNNNNNGC 7 cut(s) 46, 667, 1096, 1205, 1658, 2185, 2216
HpyF3I CTNAG 7 cut(s) 272, 364, 657, 1128, 1536, 1936, 1992
HpySE526I ACGT 6 cut(s) 409, 1243, 1503, 1570, 1627, 1849
Hsp92I GRCGYC 2 cut(s) 704, 1895
HspAI GCGC 2 cut(s) 136, 2210
Kpn2I TCCGGA 1 cut(s) 935
KroI GCCGGC 1 cut(s) 2136
KroNI GCCGGC 1 cut(s) 2138
Ksp22I TGATCA 3 cut(s) 796, 949, 1105
KspAI GTTAAC 1 cut(s) 1231
LguI GCTCTTC 1 cut(s) 154
LmnI GCTCC 7 cut(s) 25, 294, 1071, 1205, 1520, 1981, 2036
Lsp1109I GCAGC 2 cut(s) 1494, 2219
LweI GCATC 2 cut(s) 692, 1699
MaeI CTAG 5 cut(s) 627, 1068, 1802, 2063, 2223
MaeII ACGT 6 cut(s) 409, 1243, 1503, 1570, 1627, 1849
MaeIII GTNAC 6 cut(s) 59, 212, 1063, 1525, 1552, 1832
MbiI CCGCTC 1 cut(s) 1523
MfeI CAATTG 1 cut(s) 846
MflI RGATCY 4 cut(s) 370, 1780, 2067, 2083
MhlI GDGCHC 6 cut(s) 84, 124, 363, 1076, 1986, 2194
MlsI TGGCCA 2 cut(s) 485, 1033
MluNI TGGCCA 2 cut(s) 485, 1033
MlyI GAGTC 3 cut(s) 208, 593, 1731
MmeI TCCRAC 1 cut(s) 1371
Mox20I TGGCCA 2 cut(s) 485, 1033
Mph1103I ATGCAT 1 cut(s) 978
MroI TCCGGA 1 cut(s) 935
MroNI GCCGGC 1 cut(s) 2136
MroXI GAANNNNTTC 2 cut(s) 1117, 1866
MscI TGGCCA 2 cut(s) 485, 1033
MslI CAYNNNNRTG 2 cut(s) 583, 1033
Msp20I TGGCCA 2 cut(s) 485, 1033
MspCI CTTAAG 1 cut(s) 1853
MspI CCGG 5 cut(s) 165, 435, 936, 1884, 2137
MspR9I CCNGG 5 cut(s) 377, 436, 924, 1093, 1210
MunI CAATTG 1 cut(s) 846
Mva1269I GAATGC 1 cut(s) 2023
MvaI CCWGG 4 cut(s) 377, 924, 1093, 1210
MvnI CGCG 1 cut(s) 2212
MwoI GCNNNNNNNGC 7 cut(s) 46, 667, 1096, 1205, 1658, 2185, 2216
NaeI GCCGGC 1 cut(s) 2138
NciI CCSGG 1 cut(s) 436
NcoI CCATGG 3 cut(s) 480, 1034, 1381
NgoMIV GCCGGC 1 cut(s) 2136
NlaIV GGNNCC 5 cut(s) 576, 1207, 1911, 2054, 2085
NmeAIII GCCGAG 1 cut(s) 222
NmuCI GTSAC 5 cut(s) 59, 212, 1063, 1525, 1832
NotI GCGGCCGC 1 cut(s) 191
NsiI ATGCAT 1 cut(s) 978
PagI TCATGA 1 cut(s) 2149
PaqCI CACCTGC 1 cut(s) 2017
PceI AGGCCT 1 cut(s) 380
PciSI GCTCTTC 1 cut(s) 154
PctI GAATGC 1 cut(s) 2023
PdiI GCCGGC 1 cut(s) 2138
PdmI GAANNNNTTC 2 cut(s) 1117, 1866
PfeI GAWTC 6 cut(s) 144, 349, 1118, 1397, 1931, 1999
PkrI GCNGC 6 cut(s) 192, 195, 555, 634, 1509, 2209
PleI GAGTC 3 cut(s) 207, 593, 1730
PmaCI CACGTG 1 cut(s) 1571
PmlI CACGTG 1 cut(s) 1571
PpsI GAGTC 3 cut(s) 207, 593, 1730
Ppu21I YACGTR 1 cut(s) 1571
PshAI GACNNNNGTC 1 cut(s) 1839
Psp124BI GAGCTC 3 cut(s) 84, 363, 1986
Psp6I CCWGG 4 cut(s) 375, 922, 1091, 1208
PspCI CACGTG 1 cut(s) 1571
PspFI CCCAGC 1 cut(s) 770
PspGI CCWGG 4 cut(s) 375, 922, 1091, 1208
PspN4I GGNNCC 5 cut(s) 576, 1207, 1911, 2054, 2085
PspPI GGNCC 4 cut(s) 337, 432, 1089, 1212
PsrI GAACNNNNNNTAC 2 cut(s) 1255, 1287
PsuI RGATCY 4 cut(s) 370, 1780, 2067, 2083
RsaI GTAC 2 cut(s) 260, 1264
RsaNI GTAC 2 cut(s) 259, 1263
RseI CAYNNNNRTG 2 cut(s) 583, 1033
SacI GAGCTC 3 cut(s) 84, 363, 1986
SapI GCTCTTC 1 cut(s) 154
SatI GCNGC 6 cut(s) 191, 194, 554, 633, 1508, 2208
Sau96I GGNCC 4 cut(s) 337, 432, 1089, 1212
SchI GAGTC 3 cut(s) 208, 593, 1731
ScrFI CCNGG 5 cut(s) 377, 436, 924, 1093, 1210
SduI GDGCHC 6 cut(s) 84, 124, 363, 1076, 1986, 2194
SfaNI GCATC 2 cut(s) 692, 1699
SfcI CTRYAG 3 cut(s) 387, 1592, 2040
SinI GGWCC 2 cut(s) 432, 1212
SmiMI CAYNNNNRTG 2 cut(s) 583, 1033
SmlI CTYRAG 7 cut(s) 71, 139, 356, 601, 639, 1655, 1853
SmoI CTYRAG 7 cut(s) 71, 139, 356, 601, 639, 1655, 1853
SpeI ACTAGT 1 cut(s) 626
SseBI AGGCCT 1 cut(s) 380
SsiI CCGC 6 cut(s) 177, 190, 194, 553, 632, 1523
SspMI CTAG 5 cut(s) 627, 1068, 1802, 2063, 2223
SstI GAGCTC 3 cut(s) 84, 363, 1986
StuI AGGCCT 1 cut(s) 380
StyD4I CCNGG 5 cut(s) 375, 434, 922, 1091, 1208
StyI CCWWGG 6 cut(s) 340, 480, 708, 1034, 1381, 2003
TaaI ACNGT 5 cut(s) 582, 1271, 1375, 1822, 2123
TaiI ACGT 6 cut(s) 412, 1246, 1506, 1573, 1630, 1852
TaqI TCGA 2 cut(s) 1492, 1777
TatI WGTACW 1 cut(s) 258
TauI GCSGC 4 cut(s) 193, 196, 556, 635
TfiI GAWTC 6 cut(s) 144, 349, 1118, 1397, 1931, 1999
TscAI CASTG 3 cut(s) 217, 585, 2128
TseFI GTSAC 5 cut(s) 59, 212, 1063, 1525, 1832
TseI GCWGC 2 cut(s) 1507, 2207
Tsp45I GTSAC 5 cut(s) 59, 212, 1063, 1525, 1832
TspRI CASTG 3 cut(s) 217, 585, 2128
Vha464I CTTAAG 1 cut(s) 1853
VpaK11BI GGWCC 2 cut(s) 432, 1212
XapI RAATTY 3 cut(s) 468, 697, 722
XcmI CCANNNNNNNNNTGG 1 cut(s) 833
XmiI GTMKAC 2 cut(s) 874, 1377
XmnI GAANNNNTTC 2 cut(s) 1117, 1866
XspI CTAG 5 cut(s) 627, 1068, 1802, 2063, 2223
Zsp2I ATGCAT 1 cut(s) 978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.