RLG00000007374

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000660
Physical Location & Seq
Reverse (-)
16222603 .. 16228051
5449 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007374

Sequence Viewer

Length: 1815 bp
ATGGACACTCCGCCGCCGCCGGAGGAGCTGCTGAAGAAGATCCAGGTACTGGAGGAGGGTCAGGAGCACCTGAAGCAGGAGATGTCCAAGCTCCGGAACAACTCCGATGCTAAGTCCGAGCACCCGAGGGCCCACTCGGTCTCGCCGCAGCGGTCCAGATTCTCGTCTTTGCCCAAGAGGAGAGTCGCCGGGGGCGGCGCCGGAGCCGGAGCCGACGCGGTGGGTTGGAAGAGAGGGTCTACTTCGTTTCGGCATTCTTCGCCGCTGCAGAGGGAGAGCCGCAGCCATGATCCTCCGACTGGCGGCAGCGGCCGCGCCGGCGATGATTTTAGAGGTGGAGGTGGTAGCGGGCCGTCGGCGGTGAACTTTACTGATAGGCAGTATCTGAATATATTGCAGTCCATTGGGCAGTCTGTGCATATTTTTGATCCTCATGGTCGTATAATTTACTGGAACAAAACAGCTGAAGCTATGTATGGTTATTCAGCGGCAGAGGCCCTTGGACGGGATCCCATTGAGTTCTTAACAGATCCCCAGGACCATGTTGTAGGACATAATATAATCCGAAGAGTCACCAAGGGGGAGAGCTGGACAGGGCACTTCCCTGTCAAGAACAAAAAGGGGGAGAGATTTTCAGCTACTATAACGAATACTCCATTCTATGATGATGATGGCACTTTTATTGGGGTTATTTGTGTGTCAAGTGATACGAGGTCATTTCAAGAAATGAGGGTCCCTTTTTCAGGGCCGACGCACCCTGAACCAGATTCAAGTTTCAGTCGTACTAAAGCTACTGTTACAACTAAACTTGGTCTTGATCCTCAGCAGCCTCTGCAAACTGCAATTGCATCAAAAATTACAAATTTGGCATCCAAGATGAGCAACAAAGTCAAGTCGAAAATTCGGGCAGGAGAAAACTATATGGATCGGGAAGGTGGGAGTGTAGATAGTCATCATTCTGATCATGGTTCGGACTATGATCATCGGGAGGATGCAAATTCAAGTGGGGCTAGCACACCTAGAGGAGATATAGCCCCATCTCCTTTTGGTCTGTTTTCCCAGATGGATGAAAAGTCTCCAGGAAAACCTTGCAGAGAATCTGGCGATGAGAGTGAAGGAAAACCGCCAATGCACAAGGTAATATCCTCAAAGGCAGAAGCATGGATCGGTAAGAAAGGGATAACTTGGCCATGGAAAGGAAATGAGCGAGAAGGGTCAGAGGATGTTGCCGTCAAGGTATTCTCCAGGCAAGAATATTCCGAAGATGTGATACTTTCCTTCAGACAAGAGGTATCTCTTATGAAAAGGCTTCGACATCCAAATGTTCTGCTTTTCATGGGAGCTGTGACTTCTCCCGAGCGTCTTTGCATCGTAACAGAGTTCCTCCCACGTGGAAGTTTGTTTCGGTTACTACAAAGAAGCACAGCAAAACTAGACTGGAGACGTCGTGTTCATATGGCTCTGGATATAGCACGAGGCATGAATTATCTTCATCATTTCAACCCTCCCATCATCCATCGAGATCTGAAGTCTTCAAATCTCCTAGTTGATAAGAACTGGACTGTTAAGGTTGGTGATTTTGGTCTTTCACGTCTAAAGCATGAAACATTTCTCACAACCAAGACTGGGAGGGGAACGCCTCAATGGATGGCACCAGAAGTTCTACGCAATGAACCCTCAGATGAGAATGATCCTGCTAGCCGGCCAACATTTCAAGAGTTGCTGGAAAAGCTTCGAGATCTGCAGAGACAATACGCTCTTCAATTCCAAGCAGCGCGGTCCGCCGCAAGTGATAGCACCCAAAAAGAACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS PAS_9 Pkinase_Tyr

Protein Analysis

605

Amino Acids

67.27

Weight (kDa)

9.18

Isoelectric Point (pI)

54.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS PF00989 127 - 236 1.1e-13 PAS fold
PAS_8 PF13188 127 - 180 4.2e-06 PAS domain
PAS_4 PF08448 133 - 239 2.7e-13 PAS fold
PAS_9 PF13426 141 - 237 2.5e-13 PAS domain
PAS_3 PF08447 148 - 233 2.2e-06 PAS fold
PK_Tyr_Ser-Thr PF07714 398 - 558 6.6e-40 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 399 - 560 1.1e-35 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1447
AccB1I GGYRCC 2 cut(s) 197, 1651
AccB7I CCANNNNNTGG 1 cut(s) 49
AccI GTMKAC 1 cut(s) 239
AccII CGCG 3 cut(s) 218, 315, 1777
AccIII TCCGGA 1 cut(s) 93
AcoI YGGCCR 3 cut(s) 310, 1187, 1703
AcsI RAATTY 3 cut(s) 862, 900, 997
AcuI CTGAAG 5 cut(s) 53, 92, 486, 1264, 1547
AcvI CACGTG 1 cut(s) 1391
AcyI GRCGYC 2 cut(s) 198, 1444
AfaI GTAC 2 cut(s) 48, 784
AgsI TTSAA 7 cut(s) 722, 771, 1002, 1501, 1536, 1715, 1763
AjiI CACGTC 1 cut(s) 1592
AjnI CCWGG 4 cut(s) 42, 534, 1078, 1244
AluBI AGCT 9 cut(s) 28, 91, 464, 470, 588, 638, 791, 1343, 1732
AluI AGCT 9 cut(s) 28, 91, 464, 470, 588, 638, 791, 1343, 1732
Alw21I GWGCWC 2 cut(s) 69, 123
Alw26I GTCTC 4 cut(s) 145, 1080, 1435, 1741
AlwNI CAGNNNCTG 3 cut(s) 49, 385, 832
Ama87I CYCGRG 2 cut(s) 124, 1355
Aor13HI TCCGGA 1 cut(s) 93
AoxI GGCC 7 cut(s) 129, 310, 350, 495, 746, 1187, 1703
ApaI GGGCCC 1 cut(s) 133
ApeKI GCWGC 7 cut(s) 28, 148, 265, 282, 306, 826, 1772
ApoI RAATTY 3 cut(s) 862, 900, 997
Asp700I GAANNNNTTC 2 cut(s) 1608, 1731
AspLEI GCGC 3 cut(s) 200, 317, 1777
AspS9I GGNCC 9 cut(s) 129, 130, 153, 350, 496, 538, 733, 746, 1779
AsuC2I CCSGG 1 cut(s) 190
AsuHPI GGTGA 3 cut(s) 373, 565, 1586
AsuNHI GCTAGC 2 cut(s) 1010, 1697
AvaI CYCGRG 2 cut(s) 124, 1355
AvaII GGWCC 4 cut(s) 153, 538, 733, 1779
BaeGI GKGCMC 2 cut(s) 133, 600
BalI TGGCCA 1 cut(s) 1189
BamHI GGATCC 1 cut(s) 508
BanI GGYRCC 2 cut(s) 197, 1651
BanII GRGCYC 1 cut(s) 133
BarI GAAGNNNNNNTAC 2 cut(s) 1254, 1286
BauI CACGAG 1 cut(s) 1473
BbrPI CACGTG 1 cut(s) 1391
BbsI GAAGAC 1 cut(s) 1524
Bbv12I GWGCWC 2 cut(s) 69, 123
BbvCI CCTCAGC 1 cut(s) 822
BbvI GCAGC 7 cut(s) 15, 160, 252, 294, 318, 838, 1784
BccI CCATC 6 cut(s) 665, 1045, 1057, 1517, 1524, 1642
BceAI ACGGC 2 cut(s) 337, 1214
BciT130I CCWGG 4 cut(s) 44, 536, 1080, 1246
BclI TGATCA 2 cut(s) 961, 979
BcnI CCSGG 1 cut(s) 190
BcoDI GTCTC 4 cut(s) 145, 1080, 1435, 1741
BfaI CTAG 5 cut(s) 1011, 1020, 1433, 1544, 1698
BfmI CTRYAG 3 cut(s) 266, 1742, 1811
BfoI RGCGCY 1 cut(s) 201
BglI GCCNNNNNGGC 1 cut(s) 318
BglII AGATCT 2 cut(s) 1522, 1738
Bme1390I CCNGG 5 cut(s) 44, 190, 536, 1080, 1246
Bme18I GGWCC 4 cut(s) 153, 538, 733, 1779
BmeT110I CYCGRG 2 cut(s) 124, 1355
BmgBI CACGTC 1 cut(s) 1592
BmgT120I GGNCC 9 cut(s) 129, 130, 153, 350, 496, 538, 733, 746, 1779
BmiI GGNNCC 8 cut(s) 131, 199, 205, 211, 510, 734, 735, 1653
BmrFI CCNGG 5 cut(s) 44, 190, 536, 1080, 1246
BmrI ACTGGG 1 cut(s) 1635
BmsI GCATC 5 cut(s) 97, 857, 878, 982, 1377
BmtI GCTAGC 2 cut(s) 1014, 1701
BmuI ACTGGG 1 cut(s) 1635
BpiI GAAGAC 1 cut(s) 1524
BpmI CTGGAG 4 cut(s) 71, 1062, 1228, 1459
Bpu10I CCTNAGC 1 cut(s) 822
BpuMI CCSGG 1 cut(s) 190
BsaAI YACGTR 1 cut(s) 1391
BsaBI GATNNNNATC 1 cut(s) 951
BsaHI GRCGYC 2 cut(s) 198, 1444
BsaI GGTCTC 1 cut(s) 145
BsaJI CCNNGG 6 cut(s) 125, 189, 499, 534, 576, 1190
BsaWI WCCGGW 1 cut(s) 93
BsaXI ACNNNNNCTCC 4 cut(s) 637, 667, 1432, 1462
Bse118I RCCGGY 2 cut(s) 317, 1701
Bse1I ACTGG 6 cut(s) 54, 304, 455, 1442, 1562, 1630
Bse3DI GCAATG 1 cut(s) 1675
Bse8I GATNNNNATC 1 cut(s) 951
BseAI TCCGGA 1 cut(s) 93
BseBI CCWGG 4 cut(s) 44, 536, 1080, 1246
BseDI CCNNGG 6 cut(s) 125, 189, 499, 534, 576, 1190
BseGI GGATG 7 cut(s) 869, 997, 1072, 1228, 1315, 1512, 1653
BseJI GATNNNNATC 1 cut(s) 951
BseMI GCAATG 1 cut(s) 1675
BseMII CTCAG 2 cut(s) 836, 1692
BseNI ACTGG 6 cut(s) 54, 304, 455, 1442, 1562, 1630
BseRI GAGGAG 4 cut(s) 38, 68, 193, 1038
BseSI GKGCMC 2 cut(s) 133, 600
BseX3I CGGCCG 1 cut(s) 310
BseXI GCAGC 7 cut(s) 15, 160, 252, 294, 318, 838, 1784
Bsh1236I CGCG 3 cut(s) 218, 315, 1777
Bsh1285I CGRYCG 1 cut(s) 313
BshFI GGCC 7 cut(s) 131, 312, 352, 497, 748, 1189, 1705
BshNI GGYRCC 2 cut(s) 197, 1651
BsiEI CGRYCG 1 cut(s) 313
BsiHKAI GWGCWC 2 cut(s) 69, 123
BsiHKCI CYCGRG 2 cut(s) 124, 1355
BsiSI CCGG 7 cut(s) 20, 94, 189, 201, 207, 318, 1702
BslFI GGGAC 1 cut(s) 719
BsmAI GTCTC 4 cut(s) 145, 1080, 1435, 1741
BsmBI CGTCTC 1 cut(s) 1435
BsmFI GGGAC 1 cut(s) 719
BsmI GAATGC 1 cut(s) 253
BsnI GGCC 7 cut(s) 131, 312, 352, 497, 748, 1189, 1705
Bso31I GGTCTC 1 cut(s) 145
BsoBI CYCGRG 2 cut(s) 124, 1355
Bsp120I GGGCCC 1 cut(s) 129
Bsp1286I GDGCHC 4 cut(s) 69, 123, 133, 600
Bsp13I TCCGGA 1 cut(s) 93
Bsp19I CCATGG 1 cut(s) 1190
BspANI GGCC 7 cut(s) 131, 312, 352, 497, 748, 1189, 1705
BspCNI CTCAG 2 cut(s) 835, 1691
BspEI TCCGGA 1 cut(s) 93
BspFNI CGCG 3 cut(s) 218, 315, 1777
BspLI GGNNCC 8 cut(s) 131, 199, 205, 211, 510, 734, 735, 1653
BspMAI CTGCAG 2 cut(s) 270, 1746
BspOI GCTAGC 2 cut(s) 1014, 1701
BspQI GCTCTTC 1 cut(s) 1764
BspT107I GGYRCC 2 cut(s) 197, 1651
BspTNI GGTCTC 1 cut(s) 145
BsrDI GCAATG 1 cut(s) 1675
BsrFI RCCGGY 2 cut(s) 317, 1701
BsrI ACTGG 6 cut(s) 54, 304, 455, 1442, 1562, 1630
BssAI RCCGGY 2 cut(s) 317, 1701
BssECI CCNNGG 6 cut(s) 125, 189, 499, 534, 576, 1190
BssNI GRCGYC 2 cut(s) 198, 1444
BssSI CACGAG 1 cut(s) 1473
BssT1I CCWWGG 3 cut(s) 499, 576, 1190
Bst2BI CACGAG 1 cut(s) 1473
Bst2UI CCWGG 4 cut(s) 44, 536, 1080, 1246
Bst4CI ACNGT 3 cut(s) 796, 1564, 1812
Bst6I CTCTTC 3 cut(s) 224, 562, 1764
BstACI GRCGYC 2 cut(s) 198, 1444
BstAPI GCANNNNNTGC 2 cut(s) 415, 832
BstBAI YACGTR 1 cut(s) 1391
BstC8I GCNNGC 5 cut(s) 319, 350, 1012, 1699, 1703
BstDEI CTNAG 3 cut(s) 111, 822, 1678
BstDSI CCRYGG 1 cut(s) 1190
BstENI CCTNNNNNAGG 2 cut(s) 74, 741
BstF5I GGATG 7 cut(s) 869, 997, 1072, 1228, 1315, 1512, 1653
BstFNI CGCG 3 cut(s) 218, 315, 1777
BstH2I RGCGCY 1 cut(s) 201
BstHHI GCGC 3 cut(s) 200, 317, 1777
BstMAI GTCTC 4 cut(s) 145, 1080, 1435, 1741
BstMCI CGRYCG 1 cut(s) 313
BstNI CCWGG 4 cut(s) 44, 536, 1080, 1246
BstSCI CCNGG 5 cut(s) 42, 188, 534, 1078, 1244
BstSFI CTRYAG 3 cut(s) 266, 1742, 1811
BstSLI GKGCMC 2 cut(s) 133, 600
BstUI CGCG 3 cut(s) 218, 315, 1777
BstV1I GCAGC 7 cut(s) 15, 160, 252, 294, 318, 838, 1784
BstV2I GAAGAC 1 cut(s) 1524
BstX2I RGATCY 5 cut(s) 39, 508, 529, 1522, 1738
BstYI RGATCY 5 cut(s) 39, 508, 529, 1522, 1738
BstZI CGGCCG 1 cut(s) 310
BsuRI GGCC 7 cut(s) 131, 312, 352, 497, 748, 1189, 1705
BtgI CCRYGG 1 cut(s) 1190
BtgZI GCGATG 2 cut(s) 336, 1119
BtrI CACGTC 1 cut(s) 1592
BtsCI GGATG 7 cut(s) 869, 997, 1072, 1228, 1315, 1512, 1653
Cac8I GCNNGC 5 cut(s) 319, 350, 1012, 1699, 1703
CaiI CAGNNNCTG 3 cut(s) 49, 385, 832
CciNI GCGGCCGC 1 cut(s) 310
CfoI GCGC 3 cut(s) 200, 317, 1777
Cfr10I RCCGGY 2 cut(s) 317, 1701
Cfr13I GGNCC 9 cut(s) 129, 130, 153, 350, 496, 538, 733, 746, 1779
CpoI CGGWCCG 1 cut(s) 1779
CseI GACGC 3 cut(s) 224, 760, 1349
Csp6I GTAC 2 cut(s) 47, 783
CspI CGGWCCG 1 cut(s) 1779
CviAII CATG 9 cut(s) 287, 434, 542, 965, 1161, 1191, 1336, 1480, 1601
CviQI GTAC 2 cut(s) 47, 783
DdeI CTNAG 3 cut(s) 111, 822, 1678
DinI GGCGCC 1 cut(s) 199
EaeI YGGCCR 3 cut(s) 310, 1187, 1703
EagI CGGCCG 1 cut(s) 310
Eam1104I CTCTTC 3 cut(s) 224, 562, 1764
EarI CTCTTC 3 cut(s) 224, 562, 1764
EciI GGCGGA 1 cut(s) 1771
EclXI CGGCCG 1 cut(s) 310
Eco130I CCWWGG 3 cut(s) 499, 576, 1190
Eco24I GRGCYC 1 cut(s) 133
Eco31I GGTCTC 1 cut(s) 145
Eco47I GGWCC 4 cut(s) 153, 538, 733, 1779
Eco52I CGGCCG 1 cut(s) 310
Eco57I CTGAAG 5 cut(s) 53, 92, 486, 1264, 1547
Eco72I CACGTG 1 cut(s) 1391
Eco88I CYCGRG 2 cut(s) 124, 1355
EcoNI CCTNNNNNAGG 2 cut(s) 74, 741
EcoO109I RGGNCCY 3 cut(s) 129, 496, 733
EcoRII CCWGG 4 cut(s) 42, 534, 1078, 1244
EcoT14I CCWWGG 3 cut(s) 499, 576, 1190
EcoT38I GRGCYC 1 cut(s) 133
EgeI GGCGCC 1 cut(s) 199
EheI GGCGCC 1 cut(s) 199
ErhI CCWWGG 3 cut(s) 499, 576, 1190
Esp3I CGTCTC 1 cut(s) 1435
FaeI CATG 9 cut(s) 290, 437, 545, 968, 1164, 1194, 1339, 1483, 1604
FaqI GGGAC 1 cut(s) 719
FatI CATG 9 cut(s) 286, 433, 541, 964, 1160, 1190, 1335, 1479, 1600
FauI CCCGC 1 cut(s) 341
FauNDI CATATG 1 cut(s) 1455
FbaI TGATCA 2 cut(s) 961, 979
FblI GTMKAC 1 cut(s) 239
FokI GGATG 7 cut(s) 856, 1004, 1079, 1235, 1302, 1499, 1660
FriOI GRGCYC 1 cut(s) 133
FspBI CTAG 5 cut(s) 1011, 1020, 1433, 1544, 1698
GlaI GCGC 3 cut(s) 199, 316, 1776
GsuI CTGGAG 4 cut(s) 71, 1062, 1228, 1459
HaeII RGCGCY 1 cut(s) 201
HaeIII GGCC 7 cut(s) 131, 312, 352, 497, 748, 1189, 1705
HapII CCGG 7 cut(s) 20, 94, 189, 201, 207, 318, 1702
HgaI GACGC 3 cut(s) 224, 760, 1349
HhaI GCGC 3 cut(s) 200, 317, 1777
Hin1I GRCGYC 2 cut(s) 198, 1444
Hin1II CATG 9 cut(s) 290, 437, 545, 968, 1164, 1194, 1339, 1483, 1604
Hin6I GCGC 3 cut(s) 198, 315, 1775
HinP1I GCGC 3 cut(s) 198, 315, 1775
HindIII AAGCTT 1 cut(s) 1730
HinfI GANTC 5 cut(s) 159, 183, 570, 767, 1097
HpaII CCGG 7 cut(s) 20, 94, 189, 201, 207, 318, 1702
HphI GGTGA 3 cut(s) 373, 565, 1586
Hpy166II GTNNAC 2 cut(s) 240, 364
Hpy8I GTNNAC 2 cut(s) 240, 364
Hpy99I CGWCG 4 cut(s) 218, 358, 754, 1449
HpyAV CCTTC 4 cut(s) 926, 1109, 1205, 1288
HpyCH4III ACNGT 3 cut(s) 796, 1564, 1812
HpyCH4IV ACGT 3 cut(s) 1390, 1444, 1591
HpyF3I CTNAG 3 cut(s) 111, 822, 1678
HpySE526I ACGT 3 cut(s) 1390, 1444, 1591
Hsp92I GRCGYC 2 cut(s) 198, 1444
Hsp92II CATG 9 cut(s) 290, 437, 545, 968, 1164, 1194, 1339, 1483, 1604
HspAI GCGC 3 cut(s) 198, 315, 1775
KasI GGCGCC 1 cut(s) 197
KflI GGGWCCC 1 cut(s) 733
Kpn2I TCCGGA 1 cut(s) 93
KroI GCCGGC 2 cut(s) 317, 1701
KroNI GCCGGC 2 cut(s) 319, 1703
Ksp22I TGATCA 2 cut(s) 961, 979
LguI GCTCTTC 1 cut(s) 1764
LmnI GCTCC 6 cut(s) 25, 64, 96, 203, 209, 1340
Lsp1109I GCAGC 7 cut(s) 15, 160, 252, 294, 318, 838, 1784
LweI GCATC 5 cut(s) 97, 857, 878, 982, 1377
MaeI CTAG 5 cut(s) 1011, 1020, 1433, 1544, 1698
MaeII ACGT 3 cut(s) 1390, 1444, 1591
MaeIII GTNAC 5 cut(s) 571, 796, 1345, 1372, 1407
MboII GAAGA 9 cut(s) 46, 49, 241, 249, 579, 1274, 1481, 1524, 1751
MfeI CAATTG 1 cut(s) 843
MflI RGATCY 5 cut(s) 39, 508, 529, 1522, 1738
MhlI GDGCHC 4 cut(s) 69, 123, 133, 600
MlsI TGGCCA 1 cut(s) 1189
MluCI AATT 8 cut(s) 444, 843, 855, 862, 900, 997, 1483, 1763
MluNI TGGCCA 1 cut(s) 1189
Mly113I GGCGCC 1 cut(s) 198
MlyI GAGTC 2 cut(s) 192, 579
MmeI TCCRAC 2 cut(s) 206, 320
Mox20I TGGCCA 1 cut(s) 1189
MreI CGCCGGCG 1 cut(s) 317
MroI TCCGGA 1 cut(s) 93
MroNI GCCGGC 2 cut(s) 317, 1701
MroXI GAANNNNTTC 2 cut(s) 1608, 1731
MscI TGGCCA 1 cut(s) 1189
MseI TTAA 2 cut(s) 524, 1566
MslI CAYNNNNRTG 1 cut(s) 1320
Msp20I TGGCCA 1 cut(s) 1189
MspA1I CMGCKG 5 cut(s) 151, 265, 309, 464, 488
MspI CCGG 7 cut(s) 20, 94, 189, 201, 207, 318, 1702
MspR9I CCNGG 5 cut(s) 44, 190, 536, 1080, 1246
MunI CAATTG 1 cut(s) 843
Mva1269I GAATGC 1 cut(s) 253
MvaI CCWGG 4 cut(s) 44, 536, 1080, 1246
MvnI CGCG 3 cut(s) 218, 315, 1777
NaeI GCCGGC 2 cut(s) 319, 1703
NarI GGCGCC 1 cut(s) 198
NciI CCSGG 1 cut(s) 190
NcoI CCATGG 1 cut(s) 1190
NdeI CATATG 1 cut(s) 1455
NgoMIV GCCGGC 2 cut(s) 317, 1701
NheI GCTAGC 2 cut(s) 1010, 1697
NlaIII CATG 9 cut(s) 290, 437, 545, 968, 1164, 1194, 1339, 1483, 1604
NlaIV GGNNCC 8 cut(s) 131, 199, 205, 211, 510, 734, 735, 1653
NmuCI GTSAC 2 cut(s) 571, 1345
NotI GCGGCCGC 1 cut(s) 310
PciSI GCTCTTC 1 cut(s) 1764
PctI GAATGC 1 cut(s) 253
PdiI GCCGGC 2 cut(s) 319, 1703
PdmI GAANNNNTTC 2 cut(s) 1608, 1731
PfeI GAWTC 3 cut(s) 159, 767, 1097
PflMI CCANNNNNTGG 1 cut(s) 49
PfoI TCCNGGA 1 cut(s) 1078
PleI GAGTC 2 cut(s) 191, 578
PluTI GGCGCC 1 cut(s) 201
PmaCI CACGTG 1 cut(s) 1391
PmlI CACGTG 1 cut(s) 1391
PpsI GAGTC 2 cut(s) 191, 578
Ppu21I YACGTR 1 cut(s) 1391
PpuMI RGGWCCY 1 cut(s) 733
Psp5II RGGWCCY 1 cut(s) 733
Psp6I CCWGG 4 cut(s) 42, 534, 1078, 1244
PspCI CACGTG 1 cut(s) 1391
PspGI CCWGG 4 cut(s) 42, 534, 1078, 1244
PspN4I GGNNCC 8 cut(s) 131, 199, 205, 211, 510, 734, 735, 1653
PspOMI GGGCCC 1 cut(s) 129
PspPI GGNCC 9 cut(s) 129, 130, 153, 350, 496, 538, 733, 746, 1779
PspPPI RGGWCCY 1 cut(s) 733
PstI CTGCAG 2 cut(s) 270, 1746
PstNI CAGNNNCTG 3 cut(s) 49, 385, 832
PsuI RGATCY 5 cut(s) 39, 508, 529, 1522, 1738
PvuII CAGCTG 1 cut(s) 464
RsaI GTAC 2 cut(s) 48, 784
RsaNI GTAC 2 cut(s) 47, 783
RseI CAYNNNNRTG 1 cut(s) 1320
Rsr2I CGGWCCG 1 cut(s) 1779
RsrII CGGWCCG 1 cut(s) 1779
SapI GCTCTTC 1 cut(s) 1764
SaqAI TTAA 2 cut(s) 524, 1566
Sau96I GGNCC 9 cut(s) 129, 130, 153, 350, 496, 538, 733, 746, 1779
SchI GAGTC 2 cut(s) 192, 579
ScrFI CCNGG 5 cut(s) 44, 190, 536, 1080, 1246
SduI GDGCHC 4 cut(s) 69, 123, 133, 600
SfaNI GCATC 5 cut(s) 97, 857, 878, 982, 1377
SfcI CTRYAG 3 cut(s) 266, 1742, 1811
SfoI GGCGCC 1 cut(s) 199
SgrAI CRCCGGYG 1 cut(s) 317
SinI GGWCC 4 cut(s) 153, 538, 733, 1779
SmiMI CAYNNNNRTG 1 cut(s) 1320
Sse9I AATT 8 cut(s) 444, 843, 855, 862, 900, 997, 1483, 1763
SspDI GGCGCC 1 cut(s) 197
SspI AATATT 1 cut(s) 1256
SspMI CTAG 5 cut(s) 1011, 1020, 1433, 1544, 1698
StyD4I CCNGG 5 cut(s) 42, 188, 534, 1078, 1244
StyI CCWWGG 3 cut(s) 499, 576, 1190
TaaI ACNGT 3 cut(s) 796, 1564, 1812
TaiI ACGT 3 cut(s) 1393, 1447, 1594
TaqI TCGA 4 cut(s) 896, 1312, 1519, 1735
TaqII GACCGA 1 cut(s) 127
TasI AATT 8 cut(s) 444, 843, 855, 862, 900, 997, 1483, 1763
TfiI GAWTC 3 cut(s) 159, 767, 1097
Tru1I TTAA 2 cut(s) 524, 1566
Tru9I TTAA 2 cut(s) 524, 1566
TseFI GTSAC 2 cut(s) 571, 1345
TseI GCWGC 7 cut(s) 28, 148, 265, 282, 306, 826, 1772
Tsp45I GTSAC 2 cut(s) 571, 1345
TspDTI ATGAA 8 cut(s) 1083, 1316, 1324, 1442, 1481, 1496, 1617, 1686
Van91I CCANNNNNTGG 1 cut(s) 49
VpaK11BI GGWCC 4 cut(s) 153, 538, 733, 1779
XagI CCTNNNNNAGG 2 cut(s) 74, 741
XapI RAATTY 3 cut(s) 862, 900, 997
XmiI GTMKAC 1 cut(s) 239
XmnI GAANNNNTTC 2 cut(s) 1608, 1731
XspI CTAG 5 cut(s) 1011, 1020, 1433, 1544, 1698
ZraI GACGTC 1 cut(s) 1445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.