Rh4AG259600

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000660
Physical Location & Seq
Forward (+)
57560836 .. 57567530
6695 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG259600.1

Sequence Viewer

Length: 2334 bp
ATGGAAACTCCGCCGCCGCCGGAGGAGCTGCTGAAGAAGATCCAGGTACTGGAGGAGGGTCAGGAGCACCTGAAGGAGGAGATGTCCAAGCTCCGGAACAACTCCTCCGATGCTAAGTCCGAGCAGCACCCGAGGGCCCACTCGGTCTCGCCGCAGCGGTCCAGATTCTCGTCTTTGCCCAAGAGGAGAGTCGCCGGGGGCGGCGCCGGCGCCGGAGCCGACGCGGTGGGTTGGAAGAGAGGGTCCACTTCGTTTCGGCATTCTTCGCCGCTGCAGAGGGAGAGCCGCAGCCATGATCCTCCGACTGGCGGCAGCGGCCGCGCCGGCGATGATTTTAGAGGTGGAGGTGGTAGCGGGCCGTCGGCGGTGAACTTTACTGATAGGCAGTATCTGAATATATTGCAGTCCATTGGGCAGTCTGTGCATATTTTTGATCCCCATGGTCGTATAATTTACTGGAACAAAACAGCTGAAGCTATGTATGGTTATTCAGCGGCAGAGGCCCTGGGACGGGATCCCATTGAGTTCTTAACAGATCCCCAGGACCATGTTGTAGGACATAATATAATCCAAAGAGTCACCAAGGGGGAGAGCTGGACAGGGCACTTCCCTGTCAAGAACAAAAAGGGGGAGAGATTTTCAGCTACTATAACGAATACTCCATTCTATGATGATGATGGCACTTTTATTGGGGTTATTTGTGTGTCAAGTGATACGAGGGCATTTCAAGAAATGAGGGTCCCTTTTTCAGGGCTGACCCACCCTGAACCAGATTCAAGCTTCAGTCGTACTAAAGCTACTGTTACAACTAAACTTGGTCTTGATCCTCAGCAGCCTCTGCAAACTGCAATTGCATCAAAAATTACAAATTTGGCATCCAAGATGAGCAACAAAGTCAAGTCGAAAATTCGGGCAGGAGAAAACTATATGGATCGGGAAGGTGGGAGTGTAGATGGTCATCATTCTGATCATGGTTTGGACTATGACCATCGGGAGGATGCAAATTCAAGTGGGGCTAGCACACCTAGAGGAGATATAGCCCCATCTCCTTTTGGTGTGTTTTCCCAGATGGATGAAAAGTCTCCAGGAAAACCTTCCAGAGATTCTGGCGATGAGAGTGAAGGAAAACCGCCAATGCACAAGGTAATATCCTCAAAGGCAGAAGCATGGATCGGTAAGAAAGGGATAACTTGGCCATGGAAAGGAAATGAGCGAGAAGGGTCAGAGGTTAAGACCCCTCGTTTTCTTTGGCCTTGGTTGCAAAATGATCAAGAGAATGATCCAGTTCCTCAGAAGAATAATTGTGGTTTAAAACCTGACAATTACGCAACTGAAAGTAATCGACATGCAACTAATGAGGCTTCAGGATCCTGGACCTCCTCATTTAATGTTAACAGCACAAGCAGTGCCAGCAGCTGTGGAAGCACCAGCAGTACTGTGAATAAGGTAGACGTGGACACTGATTGCTTGGATTACGAAATCTTGTGGGAAGACTTGATAATTGGAGAACAGATTGGGCAAGGTTCATGTGGAACTGTATACCATGGTCTGTGGTACGGATCAGATGTTGCCGTCAAGGTATTCTCCAGGCAAGAATATTCAGAAGATGTGATACTTTCCTTCAGACAAGAGGTATCTCTTATGAAAAGGCTTCGACATCCAAATGTTCTGCTTTTCATGGGAGCTGTGACTTCTCCCGAGCGTCTTTGCATTGTAACAGAGTTCCTCCCACGTGGCAGTTTGTTTCGGTTACTACAAAGAAGCACAGCAAAACTAGACTGGAGAAGACGTATTCATATGGCTCTGGATATAGCACGAGGCATGAATTATCTTCATCATTTCAACCCTCCAATCATCCATCGAGATCTGAAGTCTTCAAATCTCCTAGTTGATAAGAACTGGACTGTCAAGGTTGGTGATTTTGGTCTTTCACGTCTAAAGCATGAAACATTTCTCACAACCAAGACTGGGAGGGGAACGCCTCAATGGATGGCACCAGAAGTTCTACGCAATGAACCCTCAGATGAGAAGTCGGATGTATATAGCTACGGAGTGATATTGTGGGAGCTTGTTACTGAAAAGATCCCTTGGGATAATCTAAACTCAATGCAGGTGATTGGAGCTGTAGGGTTCATGAACCAAAGACTAGAGATCCCAAAAGATATTGACCCGCAATGGATTTCTTTAATTGAGAGCTGCTGGCAAAGTGATCCCGCTAGCCGGCCAACATTTCAAGAGCTGCTGGAAAAGCTTCGAGATCTGCAGAGACAATACGCTCTTCAATTCCAAGCAGCGCGGTCCGCCGCAAGTGATAGCACCCAAAAAGAACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS PAS_9 Pkinase_Tyr

Protein Analysis

777

Amino Acids

86.6

Weight (kDa)

6.58

Isoelectric Point (pI)

51.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS PF00989 129 - 238 1.2e-13 PAS fold
PAS_8 PF13188 129 - 182 6e-06 PAS domain
PAS_4 PF08448 135 - 242 5e-13 PAS fold
PAS_9 PF13426 143 - 239 5e-13 PAS domain
PAS_3 PF08447 150 - 235 1.7e-06 PAS fold
PK_Tyr_Ser-Thr PF07714 501 - 751 4e-68 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 502 - 749 6.9e-58 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2101
Acc36I ACCTGC 1 cut(s) 2101
AccB1I GGYRCC 3 cut(s) 203, 209, 1993
AccB7I CCANNNNNTGG 1 cut(s) 49
AccI GTMKAC 2 cut(s) 1449, 1539
AccII CGCG 3 cut(s) 224, 321, 2296
AccIII TCCGGA 1 cut(s) 93
AcoI YGGCCR 3 cut(s) 316, 1193, 2222
AcsI RAATTY 3 cut(s) 868, 906, 1003
AcuI CTGAAG 7 cut(s) 53, 92, 492, 766, 1347, 1606, 1889
AcvI CACGTG 1 cut(s) 1733
AcyI GRCGYC 2 cut(s) 204, 210
AfaI GTAC 4 cut(s) 48, 790, 1435, 1556
AgsI TTSAA 7 cut(s) 728, 777, 1008, 1843, 1878, 2234, 2282
AjiI CACGTC 2 cut(s) 1453, 1934
AjnI CCWGG 6 cut(s) 42, 504, 540, 1084, 1370, 1586
AloI GAACNNNNNNTCC 2 cut(s) 89, 121
Alw21I GWGCWC 1 cut(s) 69
Alw26I GTCTC 3 cut(s) 151, 1086, 2260
AlwNI CAGNNNCTG 4 cut(s) 49, 391, 838, 1416
Ama87I CYCGRG 2 cut(s) 130, 1697
Aor13HI TCCGGA 1 cut(s) 93
AoxI GGCC 7 cut(s) 135, 316, 356, 501, 1193, 1250, 2222
ApaI GGGCCC 1 cut(s) 139
ApoI RAATTY 3 cut(s) 868, 906, 1003
Asp700I GAANNNNTTC 3 cut(s) 1093, 1950, 2250
AspLEI GCGC 4 cut(s) 206, 212, 323, 2296
AsuC2I CCSGG 1 cut(s) 196
AsuHPI GGTGA 4 cut(s) 379, 571, 1928, 2125
AsuNHI GCTAGC 2 cut(s) 1016, 2216
AvaI CYCGRG 2 cut(s) 130, 1697
AvaII GGWCC 6 cut(s) 159, 243, 544, 739, 1374, 2298
BaeGI GKGCMC 2 cut(s) 139, 606
BalI TGGCCA 1 cut(s) 1195
BamHI GGATCC 2 cut(s) 514, 1367
BanI GGYRCC 3 cut(s) 203, 209, 1993
BanII GRGCYC 1 cut(s) 139
BarI GAAGNNNNNNTAC 2 cut(s) 1596, 1628
BauI CACGAG 1 cut(s) 1815
BbrPI CACGTG 1 cut(s) 1733
BbsI GAAGAC 3 cut(s) 1497, 1792, 1866
Bbv12I GWGCWC 1 cut(s) 69
BbvCI CCTCAGC 1 cut(s) 828
BccI CCATC 7 cut(s) 671, 947, 996, 1051, 1063, 1866, 1984
BceAI ACGGC 2 cut(s) 343, 1556
BciT130I CCWGG 6 cut(s) 44, 506, 542, 1086, 1372, 1588
BclI TGATCA 2 cut(s) 967, 1267
BcnI CCSGG 1 cut(s) 196
BcoDI GTCTC 3 cut(s) 151, 1086, 2260
BfaI CTAG 6 cut(s) 1017, 1026, 1775, 1886, 2147, 2217
BfmI CTRYAG 4 cut(s) 272, 2124, 2261, 2330
BfoI RGCGCY 2 cut(s) 207, 213
BfuAI ACCTGC 1 cut(s) 2101
BglI GCCNNNNNGGC 1 cut(s) 324
BglII AGATCT 2 cut(s) 1864, 2257
BmcAI AGTACT 1 cut(s) 1435
Bme1390I CCNGG 7 cut(s) 44, 196, 506, 542, 1086, 1372, 1588
Bme18I GGWCC 6 cut(s) 159, 243, 544, 739, 1374, 2298
BmeT110I CYCGRG 2 cut(s) 130, 1697
BmgBI CACGTC 2 cut(s) 1453, 1934
BmrFI CCNGG 7 cut(s) 44, 196, 506, 542, 1086, 1372, 1588
BmrI ACTGGG 1 cut(s) 1977
BmsI GCATC 4 cut(s) 100, 863, 884, 988
BmtI GCTAGC 2 cut(s) 1020, 2220
BmuI ACTGGG 1 cut(s) 1977
BpiI GAAGAC 3 cut(s) 1497, 1792, 1866
BpmI CTGGAG 4 cut(s) 71, 1068, 1570, 1801
Bpu10I CCTNAGC 1 cut(s) 828
BpuMI CCSGG 1 cut(s) 196
BsaAI YACGTR 1 cut(s) 1733
BsaBI GATNNNNATC 1 cut(s) 957
BsaHI GRCGYC 2 cut(s) 204, 210
BsaI GGTCTC 1 cut(s) 151
BsaWI WCCGGW 1 cut(s) 93
BsaXI ACNNNNNCTCC 8 cut(s) 68, 89, 98, 119, 643, 673, 1774, 1804
Bse118I RCCGGY 3 cut(s) 206, 323, 2220
Bse1I ACTGG 7 cut(s) 54, 310, 461, 1283, 1784, 1904, 1972
Bse3DI GCAATG 2 cut(s) 2017, 2180
Bse8I GATNNNNATC 1 cut(s) 957
BseAI TCCGGA 1 cut(s) 93
BseBI CCWGG 6 cut(s) 44, 506, 542, 1086, 1372, 1588
BseGI GGATG 7 cut(s) 875, 1003, 1078, 1657, 1854, 1995, 2041
BseJI GATNNNNATC 1 cut(s) 957
BseMI GCAATG 2 cut(s) 2017, 2180
BseMII CTCAG 3 cut(s) 842, 1304, 2034
BseNI ACTGG 7 cut(s) 54, 310, 461, 1283, 1784, 1904, 1972
BseRI GAGGAG 7 cut(s) 38, 68, 92, 94, 199, 1044, 1369
BseSI GKGCMC 2 cut(s) 139, 606
BseX3I CGGCCG 1 cut(s) 316
Bsh1236I CGCG 3 cut(s) 224, 321, 2296
Bsh1285I CGRYCG 1 cut(s) 319
BshFI GGCC 7 cut(s) 137, 318, 358, 503, 1195, 1252, 2224
BshNI GGYRCC 3 cut(s) 203, 209, 1993
BsiEI CGRYCG 1 cut(s) 319
BsiHKAI GWGCWC 1 cut(s) 69
BsiHKCI CYCGRG 2 cut(s) 130, 1697
BsiSI CCGG 7 cut(s) 20, 94, 195, 207, 213, 324, 2221
BslFI GGGAC 2 cut(s) 522, 725
BsmAI GTCTC 3 cut(s) 151, 1086, 2260
BsmFI GGGAC 2 cut(s) 522, 725
BsmI GAATGC 1 cut(s) 259
BsnI GGCC 7 cut(s) 137, 318, 358, 503, 1195, 1252, 2224
Bso31I GGTCTC 1 cut(s) 151
BsoBI CYCGRG 2 cut(s) 130, 1697
Bsp120I GGGCCC 1 cut(s) 135
Bsp1286I GDGCHC 3 cut(s) 69, 139, 606
Bsp13I TCCGGA 1 cut(s) 93
Bsp19I CCATGG 3 cut(s) 439, 1196, 1543
BspANI GGCC 7 cut(s) 137, 318, 358, 503, 1195, 1252, 2224
BspCNI CTCAG 3 cut(s) 841, 1303, 2033
BspEI TCCGGA 1 cut(s) 93
BspFNI CGCG 3 cut(s) 224, 321, 2296
BspHI TCATGA 1 cut(s) 2133
BspMAI CTGCAG 2 cut(s) 276, 2265
BspMI ACCTGC 1 cut(s) 2101
BspOI GCTAGC 2 cut(s) 1020, 2220
BspQI GCTCTTC 1 cut(s) 2283
BspT107I GGYRCC 3 cut(s) 203, 209, 1993
BspTNI GGTCTC 1 cut(s) 151
BsrDI GCAATG 2 cut(s) 2017, 2180
BsrFI RCCGGY 3 cut(s) 206, 323, 2220
BsrI ACTGG 7 cut(s) 54, 310, 461, 1283, 1784, 1904, 1972
BssAI RCCGGY 3 cut(s) 206, 323, 2220
BssNAI GTATAC 1 cut(s) 1540
BssNI GRCGYC 2 cut(s) 204, 210
BssSI CACGAG 1 cut(s) 1815
BssT1I CCWWGG 6 cut(s) 439, 582, 1196, 1253, 1543, 2087
Bst1107I GTATAC 1 cut(s) 1540
Bst2BI CACGAG 1 cut(s) 1815
Bst2UI CCWGG 6 cut(s) 44, 506, 542, 1086, 1372, 1588
Bst4CI ACNGT 5 cut(s) 802, 1438, 1537, 1906, 2331
Bst6I CTCTTC 2 cut(s) 230, 2283
BstACI GRCGYC 2 cut(s) 204, 210
BstAPI GCANNNNNTGC 2 cut(s) 421, 838
BstBAI YACGTR 1 cut(s) 1733
BstC8I GCNNGC 8 cut(s) 208, 325, 356, 1018, 1411, 2201, 2218, 2222
BstDEI CTNAG 4 cut(s) 114, 828, 1290, 2020
BstDSI CCRYGG 3 cut(s) 439, 1196, 1543
BstENI CCTNNNNNAGG 2 cut(s) 74, 747
BstF5I GGATG 7 cut(s) 875, 1003, 1078, 1657, 1854, 1995, 2041
BstFNI CGCG 3 cut(s) 224, 321, 2296
BstH2I RGCGCY 2 cut(s) 207, 213
BstHHI GCGC 4 cut(s) 206, 212, 323, 2296
BstMAI GTCTC 3 cut(s) 151, 1086, 2260
BstMCI CGRYCG 1 cut(s) 319
BstNI CCWGG 6 cut(s) 44, 506, 542, 1086, 1372, 1588
BstNSI RCATGY 1 cut(s) 1349
BstSCI CCNGG 7 cut(s) 42, 194, 504, 540, 1084, 1370, 1586
BstSFI CTRYAG 4 cut(s) 272, 2124, 2261, 2330
BstSLI GKGCMC 2 cut(s) 139, 606
BstUI CGCG 3 cut(s) 224, 321, 2296
BstV2I GAAGAC 3 cut(s) 1497, 1792, 1866
BstX2I RGATCY 8 cut(s) 39, 514, 535, 1367, 1864, 2082, 2151, 2257
BstYI RGATCY 8 cut(s) 39, 514, 535, 1367, 1864, 2082, 2151, 2257
BstZ17I GTATAC 1 cut(s) 1540
BstZI CGGCCG 1 cut(s) 316
BsuRI GGCC 7 cut(s) 137, 318, 358, 503, 1195, 1252, 2224
BtgI CCRYGG 3 cut(s) 439, 1196, 1543
BtgZI GCGATG 2 cut(s) 342, 1125
BtrI CACGTC 2 cut(s) 1453, 1934
BtsCI GGATG 7 cut(s) 875, 1003, 1078, 1657, 1854, 1995, 2041
BtsI GCAGTG 1 cut(s) 1411
BtsIMutI CAGTG 2 cut(s) 1411, 1458
BveI ACCTGC 1 cut(s) 2101
Cac8I GCNNGC 8 cut(s) 208, 325, 356, 1018, 1411, 2201, 2218, 2222
CaiI CAGNNNCTG 4 cut(s) 49, 391, 838, 1416
CciI TCATGA 1 cut(s) 2133
CciNI GCGGCCGC 1 cut(s) 316
CfoI GCGC 4 cut(s) 206, 212, 323, 2296
Cfr10I RCCGGY 3 cut(s) 206, 323, 2220
CpoI CGGWCCG 1 cut(s) 2298
CseI GACGC 2 cut(s) 230, 1691
Csp6I GTAC 4 cut(s) 47, 789, 1434, 1555
CspI CGGWCCG 1 cut(s) 2298
CviQI GTAC 4 cut(s) 47, 789, 1434, 1555
DdeI CTNAG 4 cut(s) 114, 828, 1290, 2020
DinI GGCGCC 2 cut(s) 205, 211
DraI TTTAAA 1 cut(s) 1311
EaeI YGGCCR 3 cut(s) 316, 1193, 2222
EagI CGGCCG 1 cut(s) 316
Eam1104I CTCTTC 2 cut(s) 230, 2283
EarI CTCTTC 2 cut(s) 230, 2283
EciI GGCGGA 1 cut(s) 2290
EclXI CGGCCG 1 cut(s) 316
Eco130I CCWWGG 6 cut(s) 439, 582, 1196, 1253, 1543, 2087
Eco24I GRGCYC 1 cut(s) 139
Eco31I GGTCTC 1 cut(s) 151
Eco47I GGWCC 6 cut(s) 159, 243, 544, 739, 1374, 2298
Eco52I CGGCCG 1 cut(s) 316
Eco57I CTGAAG 7 cut(s) 53, 92, 492, 766, 1347, 1606, 1889
Eco72I CACGTG 1 cut(s) 1733
Eco88I CYCGRG 2 cut(s) 130, 1697
EcoNI CCTNNNNNAGG 2 cut(s) 74, 747
EcoO109I RGGNCCY 3 cut(s) 135, 502, 739
EcoRII CCWGG 6 cut(s) 42, 504, 540, 1084, 1370, 1586
EcoT14I CCWWGG 6 cut(s) 439, 582, 1196, 1253, 1543, 2087
EcoT38I GRGCYC 1 cut(s) 139
EgeI GGCGCC 2 cut(s) 205, 211
EheI GGCGCC 2 cut(s) 205, 211
ErhI CCWWGG 6 cut(s) 439, 582, 1196, 1253, 1543, 2087
FaqI GGGAC 2 cut(s) 522, 725
FauI CCCGC 3 cut(s) 347, 2178, 2221
FauNDI CATATG 1 cut(s) 1797
FbaI TGATCA 2 cut(s) 967, 1267
FblI GTMKAC 2 cut(s) 1449, 1539
FokI GGATG 7 cut(s) 862, 1010, 1085, 1644, 1841, 2002, 2048
FriOI GRGCYC 1 cut(s) 139
FspBI CTAG 6 cut(s) 1017, 1026, 1775, 1886, 2147, 2217
GlaI GCGC 4 cut(s) 205, 211, 322, 2295
GsuI CTGGAG 4 cut(s) 71, 1068, 1570, 1801
HaeII RGCGCY 2 cut(s) 207, 213
HaeIII GGCC 7 cut(s) 137, 318, 358, 503, 1195, 1252, 2224
HapII CCGG 7 cut(s) 20, 94, 195, 207, 213, 324, 2221
HgaI GACGC 2 cut(s) 230, 1691
HhaI GCGC 4 cut(s) 206, 212, 323, 2296
Hin1I GRCGYC 2 cut(s) 204, 210
Hin6I GCGC 4 cut(s) 204, 210, 321, 2294
HinP1I GCGC 4 cut(s) 204, 210, 321, 2294
HincII GTYRAC 1 cut(s) 1393
HindII GTYRAC 1 cut(s) 1393
HindIII AAGCTT 2 cut(s) 778, 2249
HinfI GANTC 5 cut(s) 165, 189, 576, 773, 1103
HpaI GTTAAC 1 cut(s) 1393
HpaII CCGG 7 cut(s) 20, 94, 195, 207, 213, 324, 2221
HphI GGTGA 4 cut(s) 379, 571, 1928, 2125
Hpy166II GTNNAC 6 cut(s) 246, 370, 1393, 1450, 1456, 1540
Hpy8I GTNNAC 6 cut(s) 246, 370, 1393, 1450, 1456, 1540
Hpy99I CGWCG 2 cut(s) 224, 364
HpyAV CCTTC 6 cut(s) 67, 932, 1104, 1115, 1211, 1630
HpyCH4III ACNGT 5 cut(s) 802, 1438, 1537, 1906, 2331
HpyCH4IV ACGT 4 cut(s) 1452, 1732, 1789, 1933
HpyF3I CTNAG 4 cut(s) 114, 828, 1290, 2020
HpySE526I ACGT 4 cut(s) 1452, 1732, 1789, 1933
Hsp92I GRCGYC 2 cut(s) 204, 210
HspAI GCGC 4 cut(s) 204, 210, 321, 2294
KasI GGCGCC 2 cut(s) 203, 209
KflI GGGWCCC 1 cut(s) 739
Kpn2I TCCGGA 1 cut(s) 93
KroI GCCGGC 3 cut(s) 206, 323, 2220
KroNI GCCGGC 3 cut(s) 208, 325, 2222
Ksp22I TGATCA 2 cut(s) 967, 1267
KspAI GTTAAC 1 cut(s) 1393
LguI GCTCTTC 1 cut(s) 2283
LmnI GCTCC 7 cut(s) 25, 64, 96, 215, 1682, 2065, 2120
LweI GCATC 4 cut(s) 100, 863, 884, 988
MaeI CTAG 6 cut(s) 1017, 1026, 1775, 1886, 2147, 2217
MaeII ACGT 4 cut(s) 1452, 1732, 1789, 1933
MaeIII GTNAC 6 cut(s) 577, 802, 1687, 1714, 1749, 2071
MfeI CAATTG 1 cut(s) 849
MflI RGATCY 8 cut(s) 39, 514, 535, 1367, 1864, 2082, 2151, 2257
MhlI GDGCHC 3 cut(s) 69, 139, 606
MlsI TGGCCA 1 cut(s) 1195
MluNI TGGCCA 1 cut(s) 1195
Mly113I GGCGCC 2 cut(s) 204, 210
MlyI GAGTC 2 cut(s) 198, 585
MmeI TCCRAC 3 cut(s) 212, 326, 2013
Mox20I TGGCCA 1 cut(s) 1195
MreI CGCCGGCG 2 cut(s) 206, 323
MroI TCCGGA 1 cut(s) 93
MroNI GCCGGC 3 cut(s) 206, 323, 2220
MroXI GAANNNNTTC 3 cut(s) 1093, 1950, 2250
MscI TGGCCA 1 cut(s) 1195
MseI TTAA 6 cut(s) 530, 1230, 1310, 1386, 1392, 2186
MslI CAYNNNNRTG 1 cut(s) 1662
Msp20I TGGCCA 1 cut(s) 1195
MspA1I CMGCKG 6 cut(s) 157, 271, 315, 470, 494, 1416
MspI CCGG 7 cut(s) 20, 94, 195, 207, 213, 324, 2221
MspR9I CCNGG 7 cut(s) 44, 196, 506, 542, 1086, 1372, 1588
MunI CAATTG 1 cut(s) 849
Mva1269I GAATGC 1 cut(s) 259
MvaI CCWGG 6 cut(s) 44, 506, 542, 1086, 1372, 1588
MvnI CGCG 3 cut(s) 224, 321, 2296
NaeI GCCGGC 3 cut(s) 208, 325, 2222
NarI GGCGCC 2 cut(s) 204, 210
NciI CCSGG 1 cut(s) 196
NcoI CCATGG 3 cut(s) 439, 1196, 1543
NdeI CATATG 1 cut(s) 1797
NgoMIV GCCGGC 3 cut(s) 206, 323, 2220
NheI GCTAGC 2 cut(s) 1016, 2216
NmuCI GTSAC 2 cut(s) 577, 1687
NotI GCGGCCGC 1 cut(s) 316
NspI RCATGY 1 cut(s) 1349
PagI TCATGA 1 cut(s) 2133
PaqCI CACCTGC 1 cut(s) 2101
PasI CCCWGGG 1 cut(s) 505
PciSI GCTCTTC 1 cut(s) 2283
PctI GAATGC 1 cut(s) 259
PdiI GCCGGC 3 cut(s) 208, 325, 2222
PdmI GAANNNNTTC 3 cut(s) 1093, 1950, 2250
PfeI GAWTC 3 cut(s) 165, 773, 1103
PflMI CCANNNNNTGG 1 cut(s) 49
PfoI TCCNGGA 2 cut(s) 1084, 1370
PleI GAGTC 2 cut(s) 197, 584
PluTI GGCGCC 2 cut(s) 207, 213
PmaCI CACGTG 1 cut(s) 1733
PmlI CACGTG 1 cut(s) 1733
PpsI GAGTC 2 cut(s) 197, 584
Ppu21I YACGTR 1 cut(s) 1733
PpuMI RGGWCCY 1 cut(s) 739
Psp5II RGGWCCY 1 cut(s) 739
Psp6I CCWGG 6 cut(s) 42, 504, 540, 1084, 1370, 1586
PspCI CACGTG 1 cut(s) 1733
PspGI CCWGG 6 cut(s) 42, 504, 540, 1084, 1370, 1586
PspOMI GGGCCC 1 cut(s) 135
PspPPI RGGWCCY 1 cut(s) 739
PstI CTGCAG 2 cut(s) 276, 2265
PstNI CAGNNNCTG 4 cut(s) 49, 391, 838, 1416
PsuI RGATCY 8 cut(s) 39, 514, 535, 1367, 1864, 2082, 2151, 2257
PvuII CAGCTG 2 cut(s) 470, 1416
RsaI GTAC 4 cut(s) 48, 790, 1435, 1556
RsaNI GTAC 4 cut(s) 47, 789, 1434, 1555
RseI CAYNNNNRTG 1 cut(s) 1662
Rsr2I CGGWCCG 1 cut(s) 2298
RsrII CGGWCCG 1 cut(s) 2298
SapI GCTCTTC 1 cut(s) 2283
SaqAI TTAA 6 cut(s) 530, 1230, 1310, 1386, 1392, 2186
ScaI AGTACT 1 cut(s) 1435
SchI GAGTC 2 cut(s) 198, 585
ScrFI CCNGG 7 cut(s) 44, 196, 506, 542, 1086, 1372, 1588
SduI GDGCHC 3 cut(s) 69, 139, 606
SfaNI GCATC 4 cut(s) 100, 863, 884, 988
SfcI CTRYAG 4 cut(s) 272, 2124, 2261, 2330
SfoI GGCGCC 2 cut(s) 205, 211
SgrAI CRCCGGYG 2 cut(s) 206, 323
SinI GGWCC 6 cut(s) 159, 243, 544, 739, 1374, 2298
SmiMI CAYNNNNRTG 1 cut(s) 1662
SspDI GGCGCC 2 cut(s) 203, 209
SspI AATATT 1 cut(s) 1598
SspMI CTAG 6 cut(s) 1017, 1026, 1775, 1886, 2147, 2217
StyD4I CCNGG 7 cut(s) 42, 194, 504, 540, 1084, 1370, 1586
StyI CCWWGG 6 cut(s) 439, 582, 1196, 1253, 1543, 2087
TaaI ACNGT 5 cut(s) 802, 1438, 1537, 1906, 2331
TaiI ACGT 4 cut(s) 1455, 1735, 1792, 1936
TaqI TCGA 5 cut(s) 902, 1342, 1654, 1861, 2254
TaqII GACCGA 1 cut(s) 133
TatI WGTACW 1 cut(s) 1433
TfiI GAWTC 3 cut(s) 165, 773, 1103
Tru1I TTAA 6 cut(s) 530, 1230, 1310, 1386, 1392, 2186
Tru9I TTAA 6 cut(s) 530, 1230, 1310, 1386, 1392, 2186
TscAI CASTG 2 cut(s) 1411, 1465
TseFI GTSAC 2 cut(s) 577, 1687
Tsp45I GTSAC 2 cut(s) 577, 1687
TspGWI ACGGA 2 cut(s) 1572, 2064
TspRI CASTG 2 cut(s) 1411, 1465
Van91I CCANNNNNTGG 1 cut(s) 49
VpaK11BI GGWCC 6 cut(s) 159, 243, 544, 739, 1374, 2298
XagI CCTNNNNNAGG 2 cut(s) 74, 747
XapI RAATTY 3 cut(s) 868, 906, 1003
XceI RCATGY 1 cut(s) 1349
XmiI GTMKAC 2 cut(s) 1449, 1539
XmnI GAANNNNTTC 3 cut(s) 1093, 1950, 2250
XspI CTAG 6 cut(s) 1017, 1026, 1775, 1886, 2147, 2217
ZrmI AGTACT 1 cut(s) 1435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.