Rh4AG259400

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000660
Physical Location & Seq
Forward (+)
57517740 .. 57522847
5108 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG259400.1

Sequence Viewer

Length: 2121 bp
ATGGATGATACACCACCAGCAGAGGAGCTTCTGAAGAAGCTTCAAGTGCTGGAAGAGGGTCACGCCTACCTCAAGCAAGAGCTCTCGGAGTATTCCTATGCCGAACTGGAGAACCAAAGGGCTCTCTCAAAAAAGTGCTCAAGATTCTCTTCCAAAAGAAGAGTCGGTGGTGGTGGCGGTGCAAGTTGGCGGCCGCCAAGTCTCAACTTCAGTGACAGGCAGTATCTCAACATATTGCAGTCAATAGGGCAGTCTGTGTACATATTTGATCTCAGTTGTCGTATAATTTACTGGAGCAAGACTGCTGGAAATCTATATGGTTATTCTGTGGCAGAGGCCCTTGGACAGAATCCCCTTGAGCTCTTAGCAGATCCCCAGGCCTTTGCTGTAGGATATAGTATAGTTCAACGTCTCACCAAGGAGGAGAGCTGGACCGGGCAGTTCCCTGTCAAGAACAAAATGGGGGAAATTTTTTCAGCCATGGCCACCAATACTCCATTTTATGATGATGATGGCACTTTCATTGGGGTTATATGTGTTTCATGGGATTTGCGGCATTTTCAAGAAACGAGGGTTCCACTGTTATGTTTGGAACCAGACTCAAGCTTCGGCCGTTGCCCTACCACTAGTGCGGCAACTCAAGTTGGTGTTGATCCTCAGCAACCTCTGCAAAATGAACTTGCATCAAGAATAACAAATTTGGCATCCAAGGTCTGCAACAAATTCAAGTCAAGAGTTAAGGATGGAAAAGAAAACTTGGATGACAAAGCTGGGAGTGGAGATAGTTGCTGGTGTGATCATGGTTCAGTTTCTCATCTCTCTGACCATTTGGAGGATGGGTGTTCAAGTGGGGCAAGCACACCGAGAGGAGATATACGTCCATCTCCTTTTGGTATGTTTGCCAAAATTAAAGACAAGTTTCCAGGTAAACCCTCCGGAGAATATGATGATCAGAGTGAAGGAAAACGCCCAATGCATAAGATAATAACCTCAAAGGCAGAAGCATGGATTGGTAAGAAGGGACTATCATGGCCATGGAAAGAAAATGAGAAAGAAGGGTCGGTGACTAGGAGCACTCATTTTGTTTGGCCCTGGTTGCATAATGATCAAGGAAAAGATTCAATACCTCAGGAGGGTAATTGTGCTTTAAAACCAGAAAATCAGGTGAATGAAAGTAATCGACCTGCAACTAATGAAGCTTCAGGAGCCTGGACCTCTTTGTTTAATGTTAACTGCACAAGAAGTGGTAGCAGTTCTGGAAGTACCAACAGTTCTGCGAAAAAGGAGGATGTGGATATTGATTGCTTGGACTATGAAGTCTTATGGGAAGACATGATAATTGGAGAGAGGATTGGGCAAGGTTCATGTGGAACTGTATACCATGGTGTGTGGGATGGATCAGATGTTGCTGTTAAGGTATTCTTCAAGCAAGAATATCCAGGTGATGTGCTTCTTTCTTTTAGACAAGAGGTATCTCTAATGAAAAGGCTTCGACATCCAAACGTGCTGCTTTTCATGGGAGCGGTGACTTCACCTCAGCGTCTCTGCATTGTAACAGAGTTCCTCCCACGTGGAAGTTTGTTTCAATTACTACAGAAGAACAAGTCAAAACTAGACTGGAGACGACGTGTTCAAATGGCTTTAGATATAGCACGAGGCATGAATTATCTTCATCATTTCAGCCCCCCTATTATTCATCGAGATCTGAAGTCTTCAAATCTCCTAGTTGATAAGAACTGGACTGTCAAGCCTCAATGGATGGCACCAGAAGTTCTGTGTAATGAATCCTCAGATGAGAAGTCTGATGTATACAGCTACGGGGTGATATTGTGGGAGCTCGCCACTGAGAAGATTCCTTGGGATGATCTGAATGCAATGCAGGTGATTGGAGCTGTAGGGTTCAGGAACCAAAGGCTAGAGATCCCAAAGGATGTGGATCCACAATGGGCTTCTATGACTGAGAGATGTTGGCACAGTGATCCAGCTAGCCGGCCAACATTTCATGAATTGCTGGAAGAACTTAAAGCGTTGCAGAGATGGCGTGCTCTCCAGTTCAAAGCAGCGCGTTCTGCCTCTAGTGATAGCACCCAAAACGAATTGCAGACCAGCCTGAAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006082 GO:0006464 GO:0006468 GO:0006629 GO:0006720 GO:0006721 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009723 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009750 GO:0009756 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010104 GO:0010105 GO:0010182 GO:0010646 GO:0010648 GO:0010817 GO:0012505 GO:0016020 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019752 GO:0023051 GO:0023052 GO:0023057 GO:0031984 GO:0034284 GO:0034285 GO:0036211 GO:0036293 GO:0040034 GO:0042175 GO:0042221 GO:0042445 GO:0042446 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0046777 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048580 GO:0048583 GO:0048585 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051302 GO:0051716 GO:0065007 GO:0065008 GO:0070297 GO:0070298 GO:0070482 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071322 GO:0071704 GO:0098827 GO:0140096 GO:1901564 GO:1901576 GO:1901700 GO:1901701 GO:1902531 GO:1902532 GO:2000026 GO:2000035 GO:2000069 GO:2000280
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAS PAS_9 Pkinase_Tyr

Protein Analysis

706

Amino Acids

79.35

Weight (kDa)

6.08

Isoelectric Point (pI)

44.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS PF00989 74 - 180 1.5e-08 PAS fold
PAS_4 PF08448 80 - 185 2.2e-06 PAS fold
PAS_9 PF13426 87 - 183 2.1e-08 PAS domain
PK_Tyr_Ser-Thr PF07714 447 - 583 1.5e-32 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 448 - 583 1.4e-27 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 582 - 674 5.2e-22 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 582 - 672 1.5e-13 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1870
AasI GACNNNNNNGTC 1 cut(s) 1316
Acc36I ACCTGC 2 cut(s) 1192, 1870
AccB1I GGYRCC 1 cut(s) 1762
AccBSI CCGCTC 1 cut(s) 1523
AccI GTMKAC 2 cut(s) 1377, 1809
AccII CGCG 1 cut(s) 2065
AccIII TCCGGA 1 cut(s) 935
AciI CCGC 6 cut(s) 177, 190, 194, 553, 632, 1523
AclWI GGATC 7 cut(s) 365, 647, 1405, 1915, 1931, 1944, 1973
AcoI YGGCCR 5 cut(s) 191, 483, 610, 1031, 1991
AcsI RAATTY 3 cut(s) 468, 697, 722
AcuI CTGAAG 4 cut(s) 53, 193, 1185, 1727
AcvI CACGTG 1 cut(s) 1571
AfaI GTAC 2 cut(s) 260, 1264
AfiI CCNNNNNNNGG 2 cut(s) 832, 1133
AflIII ACRYGT 1 cut(s) 1627
AhlI ACTAGT 1 cut(s) 626
AjiI CACGTC 1 cut(s) 1628
AjnI CCWGG 5 cut(s) 375, 922, 1091, 1208, 1438
AjuI GAANNNNNNNTTGG 2 cut(s) 993, 1025
Alw21I GWGCWC 6 cut(s) 84, 140, 363, 1076, 1839, 2047
Alw26I GTCTC 4 cut(s) 206, 416, 1547, 1615
AlwI GGATC 7 cut(s) 365, 647, 1405, 1915, 1931, 1944, 1973
Aor13HI TCCGGA 1 cut(s) 935
AoxI GGCC 8 cut(s) 191, 336, 378, 483, 610, 1031, 1088, 1991
ApeKI GCWGC 2 cut(s) 1507, 2060
ApoI RAATTY 3 cut(s) 468, 697, 722
ArsI GACNNNNNNTTYG 4 cut(s) 147, 179, 590, 622
Asp700I GAANNNNTTC 1 cut(s) 1117
AspLEI GCGC 1 cut(s) 2065
AspS9I GGNCC 4 cut(s) 337, 432, 1089, 1212
AsuC2I CCSGG 1 cut(s) 436
AsuHPI GGTGA 8 cut(s) 406, 1075, 1177, 1454, 1524, 1537, 1834, 1894
AsuNHI GCTAGC 1 cut(s) 1985
AvaII GGWCC 2 cut(s) 432, 1212
AxyI CCTNAGG 1 cut(s) 1128
BalI TGGCCA 2 cut(s) 485, 1033
BamHI GGATCC 1 cut(s) 1936
BanI GGYRCC 1 cut(s) 1762
BanII GRGCYC 4 cut(s) 84, 124, 363, 1839
BauI CACGAG 1 cut(s) 1653
BbrPI CACGTG 1 cut(s) 1571
BbsI GAAGAC 2 cut(s) 1335, 1704
Bbv12I GWGCWC 6 cut(s) 84, 140, 363, 1076, 1839, 2047
BbvCI CCTCAGC 2 cut(s) 657, 1536
BbvI GCAGC 2 cut(s) 1494, 2072
BccI CCATC 7 cut(s) 506, 737, 830, 889, 1388, 1753, 2031
BceAI ACGGC 1 cut(s) 597
BciT130I CCWGG 5 cut(s) 377, 924, 1093, 1210, 1440
BclI TGATCA 3 cut(s) 796, 949, 1105
BcnI CCSGG 1 cut(s) 436
BcoDI GTCTC 4 cut(s) 206, 416, 1547, 1615
BcuI ACTAGT 1 cut(s) 626
BfaI CTAG 7 cut(s) 627, 1068, 1613, 1724, 1916, 1986, 2076
BfmI CTRYAG 3 cut(s) 387, 1592, 1893
BfuAI ACCTGC 2 cut(s) 1192, 1870
BglII AGATCT 1 cut(s) 1702
BisI GCNGC 6 cut(s) 191, 194, 554, 633, 1508, 2061
BlsI GCNGC 6 cut(s) 192, 195, 555, 634, 1509, 2062
Bme1390I CCNGG 6 cut(s) 377, 436, 924, 1093, 1210, 1440
Bme18I GGWCC 2 cut(s) 432, 1212
BmgBI CACGTC 1 cut(s) 1628
BmgT120I GGNCC 4 cut(s) 337, 432, 1089, 1212
BmiI GGNNCC 6 cut(s) 576, 594, 1207, 1764, 1907, 1938
BmrFI CCNGG 6 cut(s) 377, 436, 924, 1093, 1210, 1440
BmsI GCATC 2 cut(s) 692, 713
BmtI GCTAGC 1 cut(s) 1989
BpiI GAAGAC 2 cut(s) 1335, 1704
BpmI CTGGAG 4 cut(s) 128, 313, 1639, 2033
Bpu10I CCTNAGC 2 cut(s) 657, 1536
BpuEI CTTGAG 5 cut(s) 56, 124, 377, 586, 624
BpuMI CCSGG 1 cut(s) 436
BsaAI YACGTR 1 cut(s) 1571
BsaBI GATNNNNATC 1 cut(s) 1935
BsaJI CCNNGG 9 cut(s) 340, 375, 417, 480, 708, 1034, 1091, 1381, 1856
BsaWI WCCGGW 1 cut(s) 935
BsaXI ACNNNNNCTCC 6 cut(s) 478, 508, 824, 854, 1612, 1642
Bsc4I CCNNNNNNNGG 2 cut(s) 832, 1133
Bse118I RCCGGY 1 cut(s) 1989
Bse1I ACTGG 5 cut(s) 111, 296, 1622, 1742, 2050
Bse21I CCTNAGG 1 cut(s) 1128
Bse3DI GCAATG 1 cut(s) 1881
Bse8I GATNNNNATC 1 cut(s) 1935
BseAI TCCGGA 1 cut(s) 935
BseBI CCWGG 5 cut(s) 377, 924, 1093, 1210, 1440
BseDI CCNNGG 9 cut(s) 340, 375, 417, 480, 708, 1034, 1091, 1381, 1856
BseJI GATNNNNATC 1 cut(s) 1935
BseLI CCNNNNNNNGG 2 cut(s) 832, 1133
BseMI GCAATG 1 cut(s) 1881
BseMII CTCAG 7 cut(s) 286, 671, 1142, 1550, 1803, 1836, 1950
BseNI ACTGG 5 cut(s) 111, 296, 1622, 1742, 2050
BseRI GAGGAG 3 cut(s) 38, 437, 882
BseX3I CGGCCG 2 cut(s) 191, 610
BseXI GCAGC 2 cut(s) 1494, 2072
BseYI CCCAGC 1 cut(s) 770
BsgI GTGCAG 1 cut(s) 1219
Bsh1236I CGCG 1 cut(s) 2065
Bsh1285I CGRYCG 2 cut(s) 194, 613
BshFI GGCC 8 cut(s) 193, 338, 380, 485, 612, 1033, 1090, 1993
BshNI GGYRCC 1 cut(s) 1762
BsiEI CGRYCG 2 cut(s) 194, 613
BsiHKAI GWGCWC 6 cut(s) 84, 140, 363, 1076, 1839, 2047
BsiSI CCGG 3 cut(s) 435, 936, 1990
BslFI GGGAC 1 cut(s) 1035
BslI CCNNNNNNNGG 2 cut(s) 832, 1133
BsmAI GTCTC 4 cut(s) 206, 416, 1547, 1615
BsmBI CGTCTC 3 cut(s) 416, 1547, 1615
BsmFI GGGAC 1 cut(s) 1035
BsmI GAATGC 1 cut(s) 1876
BsnI GGCC 8 cut(s) 193, 338, 380, 485, 612, 1033, 1090, 1993
Bsp1286I GDGCHC 7 cut(s) 84, 124, 140, 363, 1076, 1839, 2047
Bsp13I TCCGGA 1 cut(s) 935
Bsp1407I TGTACA 1 cut(s) 258
Bsp19I CCATGG 3 cut(s) 480, 1034, 1381
BspACI CCGC 6 cut(s) 177, 190, 194, 553, 632, 1523
BspANI GGCC 8 cut(s) 193, 338, 380, 485, 612, 1033, 1090, 1993
BspCNI CTCAG 7 cut(s) 285, 670, 1141, 1549, 1802, 1837, 1951
BspEI TCCGGA 1 cut(s) 935
BspFNI CGCG 1 cut(s) 2065
BspHI TCATGA 1 cut(s) 2002
BspLI GGNNCC 6 cut(s) 576, 594, 1207, 1764, 1907, 1938
BspMI ACCTGC 2 cut(s) 1192, 1870
BspOI GCTAGC 1 cut(s) 1989
BspPI GGATC 7 cut(s) 365, 647, 1405, 1915, 1931, 1944, 1973
BspT107I GGYRCC 1 cut(s) 1762
BsrBI CCGCTC 1 cut(s) 1523
BsrDI GCAATG 1 cut(s) 1881
BsrFI RCCGGY 1 cut(s) 1989
BsrGI TGTACA 1 cut(s) 258
BsrI ACTGG 5 cut(s) 111, 296, 1622, 1742, 2050
BssAI RCCGGY 1 cut(s) 1989
BssECI CCNNGG 9 cut(s) 340, 375, 417, 480, 708, 1034, 1091, 1381, 1856
BssNAI GTATAC 2 cut(s) 1378, 1810
BssSI CACGAG 1 cut(s) 1653
BssT1I CCWWGG 7 cut(s) 340, 417, 480, 708, 1034, 1381, 1856
Bst1107I GTATAC 2 cut(s) 1378, 1810
Bst2BI CACGAG 1 cut(s) 1653
Bst2UI CCWGG 5 cut(s) 377, 924, 1093, 1210, 1440
Bst4CI ACNGT 5 cut(s) 582, 1271, 1375, 1744, 1976
Bst6I CTCTTC 3 cut(s) 48, 154, 154
BstAPI GCANNNNNTGC 1 cut(s) 667
BstAUI TGTACA 1 cut(s) 258
BstBAI YACGTR 1 cut(s) 1571
BstC8I GCNNGC 5 cut(s) 856, 1839, 1987, 1991, 2043
BstDEI CTNAG 8 cut(s) 272, 364, 657, 1128, 1536, 1789, 1845, 1959
BstDSI CCRYGG 3 cut(s) 480, 1034, 1381
BstFNI CGCG 1 cut(s) 2065
BstHHI GCGC 1 cut(s) 2065
BstMAI GTCTC 4 cut(s) 206, 416, 1547, 1615
BstMCI CGRYCG 2 cut(s) 194, 613
BstMWI GCNNNNNNNGC 6 cut(s) 46, 667, 1096, 1205, 2038, 2069
BstNI CCWGG 5 cut(s) 377, 924, 1093, 1210, 1440
BstSCI CCNGG 6 cut(s) 375, 434, 922, 1091, 1208, 1438
BstSFI CTRYAG 3 cut(s) 387, 1592, 1893
BstUI CGCG 1 cut(s) 2065
BstV1I GCAGC 2 cut(s) 1494, 2072
BstV2I GAAGAC 2 cut(s) 1335, 1704
BstX2I RGATCY 4 cut(s) 370, 1702, 1920, 1936
BstYI RGATCY 4 cut(s) 370, 1702, 1920, 1936
BstZ17I GTATAC 2 cut(s) 1378, 1810
BstZI CGGCCG 2 cut(s) 191, 610
Bsu36I CCTNAGG 1 cut(s) 1128
BsuRI GGCC 8 cut(s) 193, 338, 380, 485, 612, 1033, 1090, 1993
BtgI CCRYGG 3 cut(s) 480, 1034, 1381
BtrI CACGTC 1 cut(s) 1628
BtsIMutI CAGTG 4 cut(s) 217, 578, 1842, 1981
BveI ACCTGC 2 cut(s) 1192, 1870
Cac8I GCNNGC 5 cut(s) 856, 1839, 1987, 1991, 2043
CciI TCATGA 1 cut(s) 2002
CciNI GCGGCCGC 1 cut(s) 191
CfoI GCGC 1 cut(s) 2065
Cfr10I RCCGGY 1 cut(s) 1989
Cfr13I GGNCC 4 cut(s) 337, 432, 1089, 1212
CseI GACGC 1 cut(s) 1529
Csp6I GTAC 2 cut(s) 259, 1263
CspCI CAANNNNNGTGG 2 cut(s) 1914, 1949
CviQI GTAC 2 cut(s) 259, 1263
DdeI CTNAG 8 cut(s) 272, 364, 657, 1128, 1536, 1789, 1845, 1959
DraI TTTAAA 1 cut(s) 1149
DrdI GACNNNNNNGTC 1 cut(s) 1316
DseDI GACNNNNNNGTC 1 cut(s) 1316
EaeI YGGCCR 5 cut(s) 191, 483, 610, 1031, 1991
EagI CGGCCG 2 cut(s) 191, 610
Eam1104I CTCTTC 3 cut(s) 48, 154, 154
EarI CTCTTC 3 cut(s) 48, 154, 154
Ecl136II GAGCTC 3 cut(s) 82, 361, 1837
EclXI CGGCCG 2 cut(s) 191, 610
Eco130I CCWWGG 7 cut(s) 340, 417, 480, 708, 1034, 1381, 1856
Eco147I AGGCCT 1 cut(s) 380
Eco24I GRGCYC 4 cut(s) 84, 124, 363, 1839
Eco47I GGWCC 2 cut(s) 432, 1212
Eco52I CGGCCG 2 cut(s) 191, 610
Eco53kI GAGCTC 3 cut(s) 82, 361, 1837
Eco57I CTGAAG 4 cut(s) 53, 193, 1185, 1727
Eco72I CACGTG 1 cut(s) 1571
Eco81I CCTNAGG 1 cut(s) 1128
EcoICRI GAGCTC 3 cut(s) 82, 361, 1837
EcoO109I RGGNCCY 1 cut(s) 337
EcoRII CCWGG 5 cut(s) 375, 922, 1091, 1208, 1438
EcoT14I CCWWGG 7 cut(s) 340, 417, 480, 708, 1034, 1381, 1856
EcoT22I ATGCAT 1 cut(s) 978
EcoT38I GRGCYC 4 cut(s) 84, 124, 363, 1839
ErhI CCWWGG 7 cut(s) 340, 417, 480, 708, 1034, 1381, 1856
Esp3I CGTCTC 3 cut(s) 416, 1547, 1615
FalI AAGNNNNNCTT 4 cut(s) 133, 165, 1406, 1438
FaqI GGGAC 1 cut(s) 1035
FbaI TGATCA 3 cut(s) 796, 949, 1105
FblI GTMKAC 2 cut(s) 1377, 1809
Fnu4HI GCNGC 6 cut(s) 191, 194, 554, 633, 1508, 2061
FriOI GRGCYC 4 cut(s) 84, 124, 363, 1839
Fsp4HI GCNGC 6 cut(s) 191, 194, 554, 633, 1508, 2061
FspBI CTAG 7 cut(s) 627, 1068, 1613, 1724, 1916, 1986, 2076
GlaI GCGC 1 cut(s) 2064
GluI GCNGC 6 cut(s) 191, 194, 554, 633, 1508, 2061
GsaI CCCAGC 1 cut(s) 774
GsuI CTGGAG 4 cut(s) 128, 313, 1639, 2033
HaeIII GGCC 8 cut(s) 193, 338, 380, 485, 612, 1033, 1090, 1993
HapII CCGG 3 cut(s) 435, 936, 1990
HgaI GACGC 1 cut(s) 1529
HhaI GCGC 1 cut(s) 2065
Hin6I GCGC 1 cut(s) 2063
HinP1I GCGC 1 cut(s) 2063
HincII GTYRAC 1 cut(s) 1231
HindII GTYRAC 1 cut(s) 1231
HindIII AAGCTT 3 cut(s) 38, 604, 1197
HinfI GANTC 7 cut(s) 144, 162, 349, 599, 1118, 1784, 1852
HpaI GTTAAC 1 cut(s) 1231
HpaII CCGG 3 cut(s) 435, 936, 1990
HphI GGTGA 8 cut(s) 406, 1075, 1177, 1454, 1524, 1537, 1834, 1894
Hpy166II GTNNAC 5 cut(s) 259, 929, 1231, 1378, 1810
Hpy188I TCNGA 9 cut(s) 33, 88, 823, 954, 1402, 1707, 1792, 1804, 1869
Hpy8I GTNNAC 5 cut(s) 259, 929, 1231, 1378, 1810
Hpy99I CGWCG 1 cut(s) 1629
HpyAV CCTTC 3 cut(s) 953, 1012, 1049
HpyCH4III ACNGT 5 cut(s) 582, 1271, 1375, 1744, 1976
HpyCH4IV ACGT 5 cut(s) 409, 877, 1503, 1570, 1627
HpyF10VI GCNNNNNNNGC 6 cut(s) 46, 667, 1096, 1205, 2038, 2069
HpyF3I CTNAG 8 cut(s) 272, 364, 657, 1128, 1536, 1789, 1845, 1959
HpySE526I ACGT 5 cut(s) 409, 877, 1503, 1570, 1627
HspAI GCGC 1 cut(s) 2063
Kpn2I TCCGGA 1 cut(s) 935
KroI GCCGGC 1 cut(s) 1989
KroNI GCCGGC 1 cut(s) 1991
Ksp22I TGATCA 3 cut(s) 796, 949, 1105
KspAI GTTAAC 1 cut(s) 1231
LmnI GCTCC 7 cut(s) 25, 294, 1071, 1205, 1520, 1834, 1889
Lsp1109I GCAGC 2 cut(s) 1494, 2072
LweI GCATC 2 cut(s) 692, 713
MaeI CTAG 7 cut(s) 627, 1068, 1613, 1724, 1916, 1986, 2076
MaeII ACGT 5 cut(s) 409, 877, 1503, 1570, 1627
MaeIII GTNAC 5 cut(s) 59, 212, 1063, 1525, 1552
MbiI CCGCTC 1 cut(s) 1523
MflI RGATCY 4 cut(s) 370, 1702, 1920, 1936
MhlI GDGCHC 7 cut(s) 84, 124, 140, 363, 1076, 1839, 2047
MlsI TGGCCA 2 cut(s) 485, 1033
MluNI TGGCCA 2 cut(s) 485, 1033
MlyI GAGTC 2 cut(s) 171, 593
Mox20I TGGCCA 2 cut(s) 485, 1033
Mph1103I ATGCAT 1 cut(s) 978
MroI TCCGGA 1 cut(s) 935
MroNI GCCGGC 1 cut(s) 1989
MroXI GAANNNNTTC 1 cut(s) 1117
MscI TGGCCA 2 cut(s) 485, 1033
MseI TTAA 7 cut(s) 738, 909, 1148, 1224, 1230, 1413, 2022
MslI CAYNNNNRTG 2 cut(s) 583, 1033
Msp20I TGGCCA 2 cut(s) 485, 1033
MspI CCGG 3 cut(s) 435, 936, 1990
MspR9I CCNGG 6 cut(s) 377, 436, 924, 1093, 1210, 1440
Mva1269I GAATGC 1 cut(s) 1876
MvaI CCWGG 5 cut(s) 377, 924, 1093, 1210, 1440
MvnI CGCG 1 cut(s) 2065
MwoI GCNNNNNNNGC 6 cut(s) 46, 667, 1096, 1205, 2038, 2069
NaeI GCCGGC 1 cut(s) 1991
NciI CCSGG 1 cut(s) 436
NcoI CCATGG 3 cut(s) 480, 1034, 1381
NgoMIV GCCGGC 1 cut(s) 1989
NheI GCTAGC 1 cut(s) 1985
NlaIV GGNNCC 6 cut(s) 576, 594, 1207, 1764, 1907, 1938
NmuCI GTSAC 4 cut(s) 59, 212, 1063, 1525
NotI GCGGCCGC 1 cut(s) 191
NsiI ATGCAT 1 cut(s) 978
PagI TCATGA 1 cut(s) 2002
PaqCI CACCTGC 1 cut(s) 1870
PceI AGGCCT 1 cut(s) 380
PctI GAATGC 1 cut(s) 1876
PdiI GCCGGC 1 cut(s) 1991
PdmI GAANNNNTTC 1 cut(s) 1117
PfeI GAWTC 5 cut(s) 144, 349, 1118, 1784, 1852
PkrI GCNGC 6 cut(s) 192, 195, 555, 634, 1509, 2062
PleI GAGTC 2 cut(s) 170, 593
PmaCI CACGTG 1 cut(s) 1571
PmlI CACGTG 1 cut(s) 1571
PpsI GAGTC 2 cut(s) 170, 593
Ppu21I YACGTR 1 cut(s) 1571
Psp124BI GAGCTC 3 cut(s) 84, 363, 1839
Psp6I CCWGG 5 cut(s) 375, 922, 1091, 1208, 1438
PspCI CACGTG 1 cut(s) 1571
PspFI CCCAGC 1 cut(s) 770
PspGI CCWGG 5 cut(s) 375, 922, 1091, 1208, 1438
PspN4I GGNNCC 6 cut(s) 576, 594, 1207, 1764, 1907, 1938
PspPI GGNCC 4 cut(s) 337, 432, 1089, 1212
PsrI GAACNNNNNNTAC 2 cut(s) 1255, 1287
PsuI RGATCY 4 cut(s) 370, 1702, 1920, 1936
RsaI GTAC 2 cut(s) 260, 1264
RsaNI GTAC 2 cut(s) 259, 1263
RseI CAYNNNNRTG 2 cut(s) 583, 1033
SacI GAGCTC 3 cut(s) 84, 363, 1839
SaqAI TTAA 7 cut(s) 738, 909, 1148, 1224, 1230, 1413, 2022
SatI GCNGC 6 cut(s) 191, 194, 554, 633, 1508, 2061
Sau96I GGNCC 4 cut(s) 337, 432, 1089, 1212
SchI GAGTC 2 cut(s) 171, 593
ScrFI CCNGG 6 cut(s) 377, 436, 924, 1093, 1210, 1440
SduI GDGCHC 7 cut(s) 84, 124, 140, 363, 1076, 1839, 2047
SfaNI GCATC 2 cut(s) 692, 713
SfcI CTRYAG 3 cut(s) 387, 1592, 1893
SinI GGWCC 2 cut(s) 432, 1212
SmiMI CAYNNNNRTG 2 cut(s) 583, 1033
SmlI CTYRAG 5 cut(s) 71, 139, 356, 601, 639
SmoI CTYRAG 5 cut(s) 71, 139, 356, 601, 639
SpeI ACTAGT 1 cut(s) 626
SseBI AGGCCT 1 cut(s) 380
SsiI CCGC 6 cut(s) 177, 190, 194, 553, 632, 1523
SspMI CTAG 7 cut(s) 627, 1068, 1613, 1724, 1916, 1986, 2076
SstI GAGCTC 3 cut(s) 84, 363, 1839
StuI AGGCCT 1 cut(s) 380
StyD4I CCNGG 6 cut(s) 375, 434, 922, 1091, 1208, 1438
StyI CCWWGG 7 cut(s) 340, 417, 480, 708, 1034, 1381, 1856
TaaI ACNGT 5 cut(s) 582, 1271, 1375, 1744, 1976
TaiI ACGT 5 cut(s) 412, 880, 1506, 1573, 1630
TaqI TCGA 3 cut(s) 1180, 1492, 1699
TatI WGTACW 1 cut(s) 258
TauI GCSGC 4 cut(s) 193, 196, 556, 635
TfiI GAWTC 5 cut(s) 144, 349, 1118, 1784, 1852
Tru1I TTAA 7 cut(s) 738, 909, 1148, 1224, 1230, 1413, 2022
Tru9I TTAA 7 cut(s) 738, 909, 1148, 1224, 1230, 1413, 2022
TscAI CASTG 4 cut(s) 217, 585, 1849, 1981
TseFI GTSAC 4 cut(s) 59, 212, 1063, 1525
TseI GCWGC 2 cut(s) 1507, 2060
Tsp45I GTSAC 4 cut(s) 59, 212, 1063, 1525
TspRI CASTG 4 cut(s) 217, 585, 1849, 1981
VpaK11BI GGWCC 2 cut(s) 432, 1212
XapI RAATTY 3 cut(s) 468, 697, 722
XcmI CCANNNNNNNNNTGG 1 cut(s) 833
XmiI GTMKAC 2 cut(s) 1377, 1809
XmnI GAANNNNTTC 1 cut(s) 1117
XspI CTAG 7 cut(s) 627, 1068, 1613, 1724, 1916, 1986, 2076
Zsp2I ATGCAT 1 cut(s) 978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.